Rroxscaffold_6G00418240

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0012275
Physical Location & Seq
Forward (+)
39796769 .. 39798206
1438 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00418240.1

Sequence Viewer

Length: 627 bp
ATGACGACGAATCAAGACGACGCATCGCAGAATCATGACCAGCAACAACAAAAACAATCCCCGCCGGTTGCTGCACCGGCTGCTGCTGACGTGGAGAGCCAGACGGAGCCGGCAGGGGCCCAGGCCCGGGCGGCTGGGAGCGGGTTTGGGGTGGCAGGGATCATACGGCGGTGGAAGAGAGATGACTTGTTGAGGAAAGGCTCTCTGGCGTTGAGAGGACTGTCCTTGGTCTTCTCTTTGCTGGCTTTCATTATCATGGCCAGTAACAAACACGGCGATTGGAAAGACTTCGATAAATATGAAGAATACAGGTATTTGTTGGGGATAGCGATATTGGCGACGTTGTACACTGGCGCACAAGCTTTCAAAAATTCCACCGGGAAAGAGATGTTTCAGCGCCGGACGTCGGCGTTGGCCGACTTCATCGGAGATCAGATTATGGCATACCTTCTGATATCATCATCATCTTCGGCGATTCCGTTAACAAATAGGATGAGGGAATCACAAGACAACATCTTTACAGACGCCTCTGCATCTGCCATTAGTATGGCTTTTCTCGCTTTCGTTTCCCTTGCACTCTTGGCCCTCATTTCCGGACACAAGTTATCTTCTCAGTCTTACATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0048037 GO:0051536 GO:0051540 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

208

Amino Acids

22.59

Weight (kDa)

7.95

Isoelectric Point (pI)

48.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 63 - 189 1.7e-26 Casparian strip membrane protein domain
MARVEL PF01284 65 - 194 2.1e-08 Membrane-associating domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012275)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G38480
fragaria_vesca FvH4_6g10520
malus_domestica MD04G1167600.v1.1 MD12G1180900.v1.1
prunus_persica Prupe.6G285800_v2.0.a1 Prupe.6G285900_v2.0.a1
pyrus_communis pycom04g14880 pycom12g17050
rosa_chinensis RchiOBHm_Chr3g0461491
rosa_laevigata RLG00000024930
rosa_multiflora Rmu_sc0002677.1_g000004
rosa_roxburghii Rroxscaffold_6G00418240 Rroxscaffold_6G00418570
rosa_rugosa Rorug03G0051200.1
rosa_samantha Rh3AG108900 Rh3BG112600 Rh3CG114300 Rh3DG114400
rosa_wichuraiana Rw3G009260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 407
AccBSI CCGCTC 1 cut(s) 141
AccIII TCCGGA 1 cut(s) 593
AciI CCGC 4 cut(s) 62, 131, 141, 169
AclWI GGATC 1 cut(s) 167
AcoI YGGCCR 2 cut(s) 258, 414
AcsI RAATTY 1 cut(s) 370
AcyI GRCGYC 2 cut(s) 404, 525
AfaI GTAC 1 cut(s) 347
AfiI CCNNNNNNNGG 3 cut(s) 126, 127, 406
AgsI TTSAA 1 cut(s) 367
AjiI CACGTC 1 cut(s) 91
AjnI CCWGG 1 cut(s) 120
AluBI AGCT 1 cut(s) 362
AluI AGCT 1 cut(s) 362
AlwI GGATC 1 cut(s) 167
Ama87I CYCGRG 1 cut(s) 126
Aor13HI TCCGGA 1 cut(s) 593
AoxI GGCC 5 cut(s) 117, 123, 258, 414, 582
ApaI GGGCCC 1 cut(s) 121
ApeKI GCWGC 3 cut(s) 71, 80, 83
ApoI RAATTY 1 cut(s) 370
Asp700I GAANNNNTTC 1 cut(s) 287
AspLEI GCGC 2 cut(s) 356, 399
AspS9I GGNCC 4 cut(s) 117, 118, 124, 583
AsuC2I CCSGG 3 cut(s) 127, 128, 379
AvaI CYCGRG 1 cut(s) 126
BaeGI GKGCMC 1 cut(s) 121
BalI TGGCCA 1 cut(s) 260
BanII GRGCYC 1 cut(s) 121
BbsI GAAGAC 1 cut(s) 223
BbvI GCAGC 3 cut(s) 58, 67, 70
BceAI ACGGC 2 cut(s) 182, 289
BciT130I CCWGG 1 cut(s) 122
BcnI CCSGG 3 cut(s) 127, 128, 379
BfoI RGCGCY 1 cut(s) 400
BglI GCCNNNNNGGC 1 cut(s) 131
BisI GCNGC 4 cut(s) 72, 81, 84, 132
BlsI GCNGC 4 cut(s) 73, 82, 85, 133
Bme1390I CCNGG 4 cut(s) 122, 127, 128, 379
BmeT110I CYCGRG 1 cut(s) 126
BmgBI CACGTC 1 cut(s) 91
BmgT120I GGNCC 4 cut(s) 117, 118, 124, 583
BmiI GGNNCC 3 cut(s) 108, 118, 119
BmrFI CCNGG 4 cut(s) 122, 127, 128, 379
BmsI GCATC 2 cut(s) 32, 542
BpiI GAAGAC 1 cut(s) 223
BpuMI CCSGG 3 cut(s) 127, 128, 379
BsaHI GRCGYC 2 cut(s) 404, 525
BsaJI CCNNGG 3 cut(s) 120, 126, 225
BsaWI WCCGGW 1 cut(s) 593
Bsc4I CCNNNNNNNGG 3 cut(s) 126, 127, 406
Bse118I RCCGGY 3 cut(s) 64, 76, 109
Bse1I ACTGG 2 cut(s) 261, 355
BseAI TCCGGA 1 cut(s) 593
BseBI CCWGG 1 cut(s) 122
BseDI CCNNGG 3 cut(s) 120, 126, 225
BseGI GGATG 1 cut(s) 498
BseLI CCNNNNNNNGG 3 cut(s) 126, 127, 406
BseMII CTCAG 1 cut(s) 626
BseNI ACTGG 2 cut(s) 261, 355
BseSI GKGCMC 1 cut(s) 121
BseXI GCAGC 3 cut(s) 58, 67, 70
BseYI CCCAGC 1 cut(s) 134
BsgI GTGCAG 1 cut(s) 57
BshFI GGCC 5 cut(s) 119, 125, 260, 416, 584
BsiHKCI CYCGRG 1 cut(s) 126
BsiSI CCGG 7 cut(s) 65, 77, 110, 127, 378, 400, 594
BslI CCNNNNNNNGG 3 cut(s) 126, 127, 406
BsnI GGCC 5 cut(s) 119, 125, 260, 416, 584
BsoBI CYCGRG 1 cut(s) 126
Bsp120I GGGCCC 1 cut(s) 117
Bsp1286I GDGCHC 1 cut(s) 121
Bsp13I TCCGGA 1 cut(s) 593
Bsp1407I TGTACA 1 cut(s) 345
Bsp143I GATC 2 cut(s) 159, 430
BspACI CCGC 4 cut(s) 62, 131, 141, 169
BspANI GGCC 5 cut(s) 119, 125, 260, 416, 584
BspCNI CTCAG 1 cut(s) 625
BspEI TCCGGA 1 cut(s) 593
BspHI TCATGA 1 cut(s) 34
BspLI GGNNCC 3 cut(s) 108, 118, 119
BspPI GGATC 1 cut(s) 167
BsrBI CCGCTC 1 cut(s) 141
BsrFI RCCGGY 3 cut(s) 64, 76, 109
BsrGI TGTACA 1 cut(s) 345
BsrI ACTGG 2 cut(s) 261, 355
BssAI RCCGGY 3 cut(s) 64, 76, 109
BssECI CCNNGG 3 cut(s) 120, 126, 225
BssMI GATC 2 cut(s) 159, 430
BssNI GRCGYC 2 cut(s) 404, 525
BssT1I CCWWGG 1 cut(s) 225
Bst2UI CCWGG 1 cut(s) 122
Bst4CI ACNGT 1 cut(s) 222
Bst6I CTCTTC 1 cut(s) 170
BstACI GRCGYC 2 cut(s) 404, 525
BstAPI GCANNNNNTGC 1 cut(s) 80
BstAUI TGTACA 1 cut(s) 345
BstC8I GCNNGC 2 cut(s) 111, 243
BstDEI CTNAG 1 cut(s) 612
BstF5I GGATG 1 cut(s) 498
BstH2I RGCGCY 1 cut(s) 400
BstHHI GCGC 2 cut(s) 356, 399
BstKTI GATC 2 cut(s) 162, 433
BstMBI GATC 2 cut(s) 159, 430
BstMWI GCNNNNNNNGC 6 cut(s) 77, 80, 131, 335, 557, 581
BstNI CCWGG 1 cut(s) 122
BstSCI CCNGG 4 cut(s) 120, 125, 126, 377
BstSLI GKGCMC 1 cut(s) 121
BstV1I GCAGC 3 cut(s) 58, 67, 70
BstV2I GAAGAC 1 cut(s) 223
BstXI CCANNNNNNTGG 1 cut(s) 547
BsuRI GGCC 5 cut(s) 119, 125, 260, 416, 584
BtgZI GCGATG 1 cut(s) 9
BtrI CACGTC 1 cut(s) 91
BtsCI GGATG 1 cut(s) 498
BtsIMutI CAGTG 1 cut(s) 348
Cac8I GCNNGC 2 cut(s) 111, 243
CciI TCATGA 1 cut(s) 34
CfoI GCGC 2 cut(s) 356, 399
Cfr10I RCCGGY 3 cut(s) 64, 76, 109
Cfr13I GGNCC 4 cut(s) 117, 118, 124, 583
Cfr9I CCCGGG 1 cut(s) 126
CseI GACGC 2 cut(s) 29, 533
Csp6I GTAC 1 cut(s) 346
CviAII CATG 2 cut(s) 35, 256
CviQI GTAC 1 cut(s) 346
DdeI CTNAG 1 cut(s) 612
DpnI GATC 2 cut(s) 161, 432
DpnII GATC 2 cut(s) 159, 430
EaeI YGGCCR 2 cut(s) 258, 414
Eam1104I CTCTTC 1 cut(s) 170
EarI CTCTTC 1 cut(s) 170
Eco130I CCWWGG 1 cut(s) 225
Eco24I GRGCYC 1 cut(s) 121
Eco32I GATATC 1 cut(s) 456
Eco88I CYCGRG 1 cut(s) 126
EcoO109I RGGNCCY 1 cut(s) 117
EcoRII CCWGG 1 cut(s) 120
EcoRV GATATC 1 cut(s) 456
EcoT14I CCWWGG 1 cut(s) 225
EcoT38I GRGCYC 1 cut(s) 121
ErhI CCWWGG 1 cut(s) 225
FaeI CATG 2 cut(s) 38, 259
FaiI YATR 7 cut(s) 36, 164, 257, 300, 440, 445, 548
FatI CATG 2 cut(s) 34, 255
FauI CCCGC 2 cut(s) 69, 134
Fnu4HI GCNGC 4 cut(s) 72, 81, 84, 132
FokI GGATG 1 cut(s) 505
FriOI GRGCYC 1 cut(s) 121
Fsp4HI GCNGC 4 cut(s) 72, 81, 84, 132
GlaI GCGC 2 cut(s) 355, 398
GluI GCNGC 4 cut(s) 72, 81, 84, 132
GsaI CCCAGC 1 cut(s) 138
HaeII RGCGCY 1 cut(s) 400
HaeIII GGCC 5 cut(s) 119, 125, 260, 416, 584
HapII CCGG 7 cut(s) 65, 77, 110, 127, 378, 400, 594
HgaI GACGC 2 cut(s) 29, 533
HhaI GCGC 2 cut(s) 356, 399
Hin1I GRCGYC 2 cut(s) 404, 525
Hin1II CATG 2 cut(s) 38, 259
Hin6I GCGC 2 cut(s) 354, 397
HinP1I GCGC 2 cut(s) 354, 397
HincII GTYRAC 1 cut(s) 483
HindII GTYRAC 1 cut(s) 483
HindIII AAGCTT 1 cut(s) 360
HinfI GANTC 4 cut(s) 10, 31, 475, 500
HpaI GTTAAC 1 cut(s) 483
HpaII CCGG 7 cut(s) 65, 77, 110, 127, 378, 400, 594
Hpy166II GTNNAC 2 cut(s) 348, 483
Hpy188I TCNGA 4 cut(s) 428, 435, 453, 626
Hpy188III TCNNGA 3 cut(s) 14, 35, 594
Hpy8I GTNNAC 2 cut(s) 348, 483
Hpy99I CGWCG 4 cut(s) 10, 23, 343, 409
HpyAV CCTTC 1 cut(s) 458
HpyCH4III ACNGT 1 cut(s) 222
HpyCH4IV ACGT 3 cut(s) 90, 341, 404
HpyCH4V TGCA 3 cut(s) 74, 533, 575
HpyF10VI GCNNNNNNNGC 6 cut(s) 77, 80, 131, 335, 557, 581
HpyF3I CTNAG 1 cut(s) 612
HpySE526I ACGT 3 cut(s) 90, 341, 404
Hsp92I GRCGYC 2 cut(s) 404, 525
Hsp92II CATG 2 cut(s) 38, 259
HspAI GCGC 2 cut(s) 354, 397
Kpn2I TCCGGA 1 cut(s) 593
KroI GCCGGC 1 cut(s) 109
KroNI GCCGGC 1 cut(s) 111
KspAI GTTAAC 1 cut(s) 483
Kzo9I GATC 2 cut(s) 159, 430
LmnI GCTCC 2 cut(s) 106, 138
Lsp1109I GCAGC 3 cut(s) 58, 67, 70
LweI GCATC 2 cut(s) 32, 542
MaeII ACGT 3 cut(s) 90, 341, 404
MaeIII GTNAC 1 cut(s) 263
MalI GATC 2 cut(s) 161, 432
MbiI CCGCTC 1 cut(s) 141
MboI GATC 2 cut(s) 159, 430
MboII GAAGA 5 cut(s) 187, 223, 314, 459, 600
MhlI GDGCHC 1 cut(s) 121
MlsI TGGCCA 1 cut(s) 260
MluCI AATT 1 cut(s) 370
MluNI TGGCCA 1 cut(s) 260
MnlI CCTC 5 cut(s) 186, 209, 489, 538, 596
Mox20I TGGCCA 1 cut(s) 260
MroI TCCGGA 1 cut(s) 593
MroNI GCCGGC 1 cut(s) 109
MroXI GAANNNNTTC 1 cut(s) 287
MscI TGGCCA 1 cut(s) 260
MseI TTAA 1 cut(s) 482
MslI CAYNNNNRTG 2 cut(s) 254, 545
Msp20I TGGCCA 1 cut(s) 260
MspI CCGG 7 cut(s) 65, 77, 110, 127, 378, 400, 594
MspR9I CCNGG 4 cut(s) 122, 127, 128, 379
MvaI CCWGG 1 cut(s) 122
MwoI GCNNNNNNNGC 6 cut(s) 77, 80, 131, 335, 557, 581
NaeI GCCGGC 1 cut(s) 111
NciI CCSGG 3 cut(s) 127, 128, 379
NdeII GATC 2 cut(s) 159, 430
NgoMIV GCCGGC 1 cut(s) 109
NlaIII CATG 2 cut(s) 38, 259
NlaIV GGNNCC 3 cut(s) 108, 118, 119
PagI TCATGA 1 cut(s) 34
PcsI WCGNNNNNNNCGW 1 cut(s) 476
PdiI GCCGGC 1 cut(s) 111
PdmI GAANNNNTTC 1 cut(s) 287
PfeI GAWTC 4 cut(s) 10, 31, 475, 500
PkrI GCNGC 4 cut(s) 73, 82, 85, 133
Psp6I CCWGG 1 cut(s) 120
PspFI CCCAGC 1 cut(s) 134
PspGI CCWGG 1 cut(s) 120
PspN4I GGNNCC 3 cut(s) 108, 118, 119
PspOMI GGGCCC 1 cut(s) 117
PspPI GGNCC 4 cut(s) 117, 118, 124, 583
RsaI GTAC 1 cut(s) 347
RsaNI GTAC 1 cut(s) 346
RseI CAYNNNNRTG 2 cut(s) 254, 545
SaqAI TTAA 1 cut(s) 482
SatI GCNGC 4 cut(s) 72, 81, 84, 132
Sau3AI GATC 2 cut(s) 159, 430
Sau96I GGNCC 4 cut(s) 117, 118, 124, 583
ScrFI CCNGG 4 cut(s) 122, 127, 128, 379
SduI GDGCHC 1 cut(s) 121
SetI ASST 6 cut(s) 93, 314, 344, 364, 407, 450
SfaNI GCATC 2 cut(s) 32, 542
SmaI CCCGGG 1 cut(s) 128
SmiMI CAYNNNNRTG 2 cut(s) 254, 545
SrfI GCCCGGGC 1 cut(s) 128
Sse9I AATT 1 cut(s) 370
SsiI CCGC 4 cut(s) 62, 131, 141, 169
StyD4I CCNGG 4 cut(s) 120, 125, 126, 377
StyI CCWWGG 1 cut(s) 225
TaaI ACNGT 1 cut(s) 222
TaiI ACGT 3 cut(s) 93, 344, 407
TaqI TCGA 1 cut(s) 291
TasI AATT 1 cut(s) 370
TatI WGTACW 1 cut(s) 345
TauI GCSGC 1 cut(s) 134
TfiI GAWTC 4 cut(s) 10, 31, 475, 500
Tru1I TTAA 1 cut(s) 482
Tru9I TTAA 1 cut(s) 482
TscAI CASTG 1 cut(s) 355
TseI GCWGC 3 cut(s) 71, 80, 83
TspDTI ATGAA 3 cut(s) 238, 315, 412
TspGWI ACGGA 2 cut(s) 119, 468
TspMI CCCGGG 1 cut(s) 126
TspRI CASTG 1 cut(s) 355
XapI RAATTY 1 cut(s) 370
XmaI CCCGGG 1 cut(s) 126
XmnI GAANNNNTTC 1 cut(s) 287
ZraI GACGTC 1 cut(s) 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.