MD12G1180900.v1.1

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0012275
Physical Location & Seq
Forward (+)
26082349 .. 26083958
1610 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1180900.v1.1.491

Sequence Viewer

Length: 633 bp
ATGACCAACCAAGACGACACGCCCCCGATACACGAAGAACGGGCAGCCGGATCACCGCCGCCAGTTGTTCCCACGGCTCCACCGGAAAATGATGTGGAGAACCAGACCCAGTCCCACGCCAGGACTACTGGGACGGGGATTGGCGTCGGGGGAATCCTGCGGCGGTGGAAGAGGGAGGACTTGTTGAGGAGAGGCTCTCTGGCTCTGAGGGGACTTGCTCTGGTTTTTTCATTGCTGGCCTTCATCATCATGGCCAGCAACAAACACGGTCACGGGAACAATTTCGACGAATATGAAGAATACAGGTATGTGTTGGCTATAGCGATTCTGGCGACGTTGTACACAGCGCTGCAATCATTCAGACATGCACGCGAGCTTTCCACCGGAAGAGAAATGTCTCAGCGACGGACGTCGGCGTTGGCTGACTTCATCGGCGATCAGATTATGGCCTATCTACTGATATCATCGTCATCATCGGCGATTCCGTTGACGAATAGAATGAGAGAATATCAAAACAACCTATTTACAGATGCTGCAGCATCTGCCATTAGCATGGCCTTTCTTGCTTTTGTTGCTCATGCACTATCGGCTATAATTTCTGGTTACAAATTATCGACTCAATCTTACATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0048037 GO:0051536 GO:0051540 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

211

Amino Acids

23.07

Weight (kDa)

7.03

Isoelectric Point (pI)

47.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 61 - 192 8.9e-30 Casparian strip membrane protein domain
MARVEL PF01284 63 - 199 4.5e-09 Membrane-associating domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012275)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G38480
fragaria_vesca FvH4_6g10520
malus_domestica MD04G1167600.v1.1 MD12G1180900.v1.1
prunus_persica Prupe.6G285800_v2.0.a1 Prupe.6G285900_v2.0.a1
pyrus_communis pycom04g14880 pycom12g17050
rosa_chinensis RchiOBHm_Chr3g0461491
rosa_laevigata RLG00000024930
rosa_multiflora Rmu_sc0002677.1_g000004
rosa_roxburghii Rroxscaffold_6G00418240 Rroxscaffold_6G00418570
rosa_rugosa Rorug03G0051200.1
rosa_samantha Rh3AG108900 Rh3BG112600 Rh3CG114300 Rh3DG114400
rosa_wichuraiana Rw3G009260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 413
AccII CGCG 1 cut(s) 372
AciI CCGC 4 cut(s) 56, 59, 160, 163
AclWI GGATC 1 cut(s) 58
AcoI YGGCCR 1 cut(s) 252
AcyI GRCGYC 2 cut(s) 144, 410
AfaI GTAC 1 cut(s) 341
AfeI AGCGCT 1 cut(s) 348
AfiI CCNNNNNNNGG 1 cut(s) 120
AjnI CCWGG 1 cut(s) 119
AluBI AGCT 1 cut(s) 376
AluI AGCT 1 cut(s) 376
Alw26I GTCTC 1 cut(s) 402
AlwI GGATC 1 cut(s) 58
AlwNI CAGNNNCTG 2 cut(s) 533, 542
Aor51HI AGCGCT 1 cut(s) 348
AoxI GGCC 4 cut(s) 237, 252, 447, 555
ApeKI GCWGC 4 cut(s) 44, 349, 533, 536
Asp700I GAANNNNTTC 1 cut(s) 281
AspLEI GCGC 1 cut(s) 349
AsuHPI GGTGA 1 cut(s) 45
BalI TGGCCA 1 cut(s) 254
BbvI GCAGC 4 cut(s) 56, 336, 520, 548
BceAI ACGGC 1 cut(s) 90
BciT130I CCWGG 1 cut(s) 121
BcoDI GTCTC 1 cut(s) 402
BfmI CTRYAG 2 cut(s) 318, 534
BfoI RGCGCY 1 cut(s) 350
BisI GCNGC 6 cut(s) 45, 59, 161, 350, 534, 537
BlsI GCNGC 6 cut(s) 46, 60, 162, 351, 535, 538
Bme1390I CCNGG 1 cut(s) 121
BmiI GGNNCC 1 cut(s) 78
BmrFI CCNGG 1 cut(s) 121
BmrI ACTGGG 2 cut(s) 103, 138
BmsI GCATC 2 cut(s) 520, 548
BmuI ACTGGG 2 cut(s) 103, 138
BoxI GACNNNNGTC 1 cut(s) 409
BplI GAGNNNNNCTC 2 cut(s) 181, 213
BsaHI GRCGYC 2 cut(s) 144, 410
BsaJI CCNNGG 1 cut(s) 72
BsaWI WCCGGW 2 cut(s) 82, 383
BsaXI ACNNNNNCTCC 2 cut(s) 167, 197
Bsc4I CCNNNNNNNGG 1 cut(s) 120
Bse1I ACTGG 3 cut(s) 62, 109, 133
Bse3DI GCAATG 1 cut(s) 230
BseBI CCWGG 1 cut(s) 121
BseDI CCNNGG 1 cut(s) 72
BseLI CCNNNNNNNGG 1 cut(s) 120
BseMI GCAATG 1 cut(s) 230
BseMII CTCAG 2 cut(s) 197, 413
BseNI ACTGG 3 cut(s) 62, 109, 133
BseRI GAGGAG 1 cut(s) 202
BseXI GCAGC 4 cut(s) 56, 336, 520, 548
Bsh1236I CGCG 1 cut(s) 372
BshFI GGCC 4 cut(s) 239, 254, 449, 557
BsiSI CCGG 3 cut(s) 48, 83, 384
BslFI GGGAC 3 cut(s) 97, 145, 225
BslI CCNNNNNNNGG 1 cut(s) 120
BsmAI GTCTC 1 cut(s) 402
BsmFI GGGAC 3 cut(s) 97, 145, 225
BsnI GGCC 4 cut(s) 239, 254, 449, 557
Bsp1407I TGTACA 1 cut(s) 339
Bsp143I GATC 2 cut(s) 50, 436
BspACI CCGC 4 cut(s) 56, 59, 160, 163
BspANI GGCC 4 cut(s) 239, 254, 449, 557
BspCNI CTCAG 2 cut(s) 198, 412
BspFNI CGCG 1 cut(s) 372
BspLI GGNNCC 1 cut(s) 78
BspMAI CTGCAG 1 cut(s) 538
BspPI GGATC 1 cut(s) 58
BsrDI GCAATG 1 cut(s) 230
BsrGI TGTACA 1 cut(s) 339
BsrI ACTGG 3 cut(s) 62, 109, 133
BssECI CCNNGG 1 cut(s) 72
BssMI GATC 2 cut(s) 50, 436
BssNI GRCGYC 2 cut(s) 144, 410
Bst2UI CCWGG 1 cut(s) 121
Bst4CI ACNGT 1 cut(s) 269
Bst6I CTCTTC 2 cut(s) 164, 382
BstACI GRCGYC 2 cut(s) 144, 410
BstAPI GCANNNNNTGC 1 cut(s) 542
BstAUI TGTACA 1 cut(s) 339
BstC8I GCNNGC 4 cut(s) 237, 256, 370, 374
BstDEI CTNAG 2 cut(s) 206, 399
BstDSI CCRYGG 1 cut(s) 72
BstFNI CGCG 1 cut(s) 372
BstH2I RGCGCY 1 cut(s) 350
BstHHI GCGC 1 cut(s) 349
BstKTI GATC 2 cut(s) 53, 439
BstMAI GTCTC 1 cut(s) 402
BstMBI GATC 2 cut(s) 50, 436
BstMWI GCNNNNNNNGC 5 cut(s) 329, 542, 563, 572, 587
BstNI CCWGG 1 cut(s) 121
BstNSI RCATGY 1 cut(s) 368
BstPAI GACNNNNGTC 1 cut(s) 409
BstSCI CCNGG 1 cut(s) 119
BstSFI CTRYAG 2 cut(s) 318, 534
BstUI CGCG 1 cut(s) 372
BstV1I GCAGC 4 cut(s) 56, 336, 520, 548
BstXI CCANNNNNNTGG 1 cut(s) 553
BsuRI GGCC 4 cut(s) 239, 254, 449, 557
BtgI CCRYGG 1 cut(s) 72
Cac8I GCNNGC 4 cut(s) 237, 256, 370, 374
CaiI CAGNNNCTG 2 cut(s) 533, 542
CfoI GCGC 1 cut(s) 349
CseI GACGC 1 cut(s) 133
Csp6I GTAC 1 cut(s) 340
CviAII CATG 4 cut(s) 250, 365, 553, 578
CviQI GTAC 1 cut(s) 340
DdeI CTNAG 2 cut(s) 206, 399
DpnI GATC 2 cut(s) 52, 438
DpnII GATC 2 cut(s) 50, 436
EaeI YGGCCR 1 cut(s) 252
Eam1104I CTCTTC 2 cut(s) 164, 382
EarI CTCTTC 2 cut(s) 164, 382
Eco32I GATATC 1 cut(s) 462
Eco47III AGCGCT 1 cut(s) 348
EcoRII CCWGG 1 cut(s) 119
EcoRV GATATC 1 cut(s) 462
FaeI CATG 4 cut(s) 253, 368, 556, 581
FaiI YATR 9 cut(s) 251, 294, 309, 320, 366, 446, 554, 579, 593
FaqI GGGAC 3 cut(s) 97, 145, 225
FatI CATG 4 cut(s) 249, 364, 552, 577
Fnu4HI GCNGC 6 cut(s) 45, 59, 161, 350, 534, 537
Fsp4HI GCNGC 6 cut(s) 45, 59, 161, 350, 534, 537
GlaI GCGC 1 cut(s) 348
GluI GCNGC 6 cut(s) 45, 59, 161, 350, 534, 537
HaeII RGCGCY 1 cut(s) 350
HaeIII GGCC 4 cut(s) 239, 254, 449, 557
HapII CCGG 3 cut(s) 48, 83, 384
HgaI GACGC 1 cut(s) 133
HhaI GCGC 1 cut(s) 349
Hin1I GRCGYC 2 cut(s) 144, 410
Hin1II CATG 4 cut(s) 253, 368, 556, 581
Hin6I GCGC 1 cut(s) 347
HinP1I GCGC 1 cut(s) 347
HincII GTYRAC 1 cut(s) 489
HindII GTYRAC 1 cut(s) 489
HinfI GANTC 4 cut(s) 153, 325, 481, 616
HpaII CCGG 3 cut(s) 48, 83, 384
HphI GGTGA 1 cut(s) 45
Hpy166II GTNNAC 2 cut(s) 342, 489
Hpy188I TCNGA 4 cut(s) 207, 362, 441, 632
Hpy8I GTNNAC 2 cut(s) 342, 489
Hpy99I CGWCG 5 cut(s) 149, 290, 337, 408, 415
HpyAV CCTTC 1 cut(s) 250
HpyCH4III ACNGT 1 cut(s) 269
HpyCH4IV ACGT 2 cut(s) 335, 410
HpyCH4V TGCA 4 cut(s) 352, 368, 536, 581
HpyF10VI GCNNNNNNNGC 5 cut(s) 329, 542, 563, 572, 587
HpyF3I CTNAG 2 cut(s) 206, 399
HpySE526I ACGT 2 cut(s) 335, 410
Hsp92I GRCGYC 2 cut(s) 144, 410
Hsp92II CATG 4 cut(s) 253, 368, 556, 581
HspAI GCGC 1 cut(s) 347
Kzo9I GATC 2 cut(s) 50, 436
LmnI GCTCC 1 cut(s) 82
Lsp1109I GCAGC 4 cut(s) 56, 336, 520, 548
LweI GCATC 2 cut(s) 520, 548
MaeII ACGT 2 cut(s) 335, 410
MaeIII GTNAC 2 cut(s) 269, 602
MalI GATC 2 cut(s) 52, 438
MboI GATC 2 cut(s) 50, 436
MboII GAAGA 4 cut(s) 47, 181, 308, 399
MlsI TGGCCA 1 cut(s) 254
MluCI AATT 3 cut(s) 280, 594, 608
MluNI TGGCCA 1 cut(s) 254
MlyI GAGTC 1 cut(s) 610
MnlI CCTC 5 cut(s) 165, 169, 180, 185, 201
Mox20I TGGCCA 1 cut(s) 254
MroXI GAANNNNTTC 1 cut(s) 281
MscI TGGCCA 1 cut(s) 254
MslI CAYNNNNRTG 2 cut(s) 248, 551
Msp20I TGGCCA 1 cut(s) 254
MspI CCGG 3 cut(s) 48, 83, 384
MspR9I CCNGG 1 cut(s) 121
MvaI CCWGG 1 cut(s) 121
MvnI CGCG 1 cut(s) 372
MwoI GCNNNNNNNGC 5 cut(s) 329, 542, 563, 572, 587
NdeII GATC 2 cut(s) 50, 436
NlaIII CATG 4 cut(s) 253, 368, 556, 581
NlaIV GGNNCC 1 cut(s) 78
NmuCI GTSAC 1 cut(s) 269
NspI RCATGY 1 cut(s) 368
PcsI WCGNNNNNNNCGW 1 cut(s) 482
PdmI GAANNNNTTC 1 cut(s) 281
PfeI GAWTC 3 cut(s) 153, 325, 481
PflFI GACNNNGTC 1 cut(s) 109
PkrI GCNGC 6 cut(s) 46, 60, 162, 351, 535, 538
PleI GAGTC 1 cut(s) 610
PpsI GAGTC 1 cut(s) 610
PshAI GACNNNNGTC 1 cut(s) 409
Psp6I CCWGG 1 cut(s) 119
PspGI CCWGG 1 cut(s) 119
PspN4I GGNNCC 1 cut(s) 78
PstI CTGCAG 1 cut(s) 538
PstNI CAGNNNCTG 2 cut(s) 533, 542
PsyI GACNNNGTC 1 cut(s) 109
RsaI GTAC 1 cut(s) 341
RsaNI GTAC 1 cut(s) 340
RseI CAYNNNNRTG 2 cut(s) 248, 551
SatI GCNGC 6 cut(s) 45, 59, 161, 350, 534, 537
Sau3AI GATC 2 cut(s) 50, 436
SchI GAGTC 1 cut(s) 610
ScrFI CCNGG 1 cut(s) 121
SetI ASST 5 cut(s) 308, 338, 378, 413, 522
SfaNI GCATC 2 cut(s) 520, 548
SfcI CTRYAG 2 cut(s) 318, 534
SmiMI CAYNNNNRTG 2 cut(s) 248, 551
Sse9I AATT 3 cut(s) 280, 594, 608
SsiI CCGC 4 cut(s) 56, 59, 160, 163
StyD4I CCNGG 1 cut(s) 119
TaaI ACNGT 1 cut(s) 269
TaiI ACGT 2 cut(s) 338, 413
TaqI TCGA 2 cut(s) 285, 614
TasI AATT 3 cut(s) 280, 594, 608
TatI WGTACW 1 cut(s) 339
TauI GCSGC 2 cut(s) 61, 163
TfiI GAWTC 3 cut(s) 153, 325, 481
TseFI GTSAC 1 cut(s) 269
TseI GCWGC 4 cut(s) 44, 349, 533, 536
Tsp45I GTSAC 1 cut(s) 269
TspDTI ATGAA 4 cut(s) 219, 232, 309, 418
TspGWI ACGGA 2 cut(s) 421, 474
Tth111I GACNNNGTC 1 cut(s) 109
XceI RCATGY 1 cut(s) 368
XmnI GAANNNNTTC 1 cut(s) 281
ZraI GACGTC 1 cut(s) 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.