MD04G1176100.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0005471
Physical Location & Seq
Forward (+)
26701571 .. 26705896
4326 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1176100.v1.1.491

Sequence Viewer

Length: 1437 bp
ATGGTTTCCTCTCAGCTACCTGGTTTAACCAAGGCCCACATGTGCAAACCAATTCAGACCCAGTTGCTTTTTTCCATGGGAACAGTGGATGCTGCAGTTTCAGATCACCTCGAGCTTGATTTTGATGATGTATTTGGCCCTCTACCAGTTCAGGCTTCAGTAGAAGTGAATGTGGCTAATTCTGCCTCGATTGAAGATGCAACTGATCTTATTTATGACGATCCAGTGGTTATTCACAACCGATCACATTCTTTGGTTGGCCCCTCATCGTTTGTAAACCAATCATTGAAGCTCAGCAAACTGACCATAAATGACACAGAAGATTCAGTGGAACTGGTAGAGTGTGTCAATGGAGACACCATCAAAGAATTTCAGGAACCTTTTATTGGTGTCGATGAAAAGCCTCTTGAAGTTATTGATGAAAACCCCATGAAGATCGAGAGTGTAGGCATTGAAGATTTTGAGGTTTTGAAAGTTGTTGGGCAGGGTGCATTTGCAAAAGTATATCAGGTGAGGAAGAAGGGGACATCAGAAATATATGCAATGAAGGTCATGCGAAAGGACAAGATCATGGAGAAGAATCATGCCGAATACATGAAAGCTGAGAGGGATATTTTAACAAAAATTGATCATCCATTCATTATCCAACTTAGATACTCATTTCAGACCAAATATAGACTATATCTTGTGCTGGATTTCGTTAATGGTGGTCACCTCTTCTTTCAGCTCTATCACCATGGCCTTTTTAGAGAGGAGCTGGGACGAATATATGCTGCTGAGATTGTTTCTGCGGTTGCTCACCTCCATGCAAATGGAATAATGCATAGGGATCTTAAACCTGAAAATATCCTTTTGGATGCAGATGGCCACGCCATGTTGACTGATTTTGGTCTTGCGAAGCAACTTGAAGAGAACACAAGATCTAATTCTATGTGTGGAACCGTAGAATACATGCCACCCGAAATAGTTCTTGGAAAGGGACATGATAAGGCTGCAGACTGGTGGAGTGTGGGAGTCCTATTGTTTGAGATGCTTACTGGAAAGCCTCCTTTTACTGGGAACAGGGAGAAAATTCAGCAGAAGATAGTTAAGGAAAAGATCAAGTTGCCTTCCTTTTTGTCAAGTGAAGCACATTCACTGTTGAAAGCGCTGCTGCAGAAGGATCCAACCAAGCGCCTCGGTCGTGGGCCTTTGGGGAGTGAGGACATCAAGCGCCACAAATGGTTCAAGCCAATAAACTGGAAAAAACTGGAGGCTCGGCAAATCCAACCAAGCTTTCGCCCAGATGTTGCCGGGAGGCACTGCATCGCCAATTTTGATAAGTGCTGGACCGACATGTCAGTTGTGGATTCCCCAGCCGCTAGCCCGAATGCTGCTGGAAACCCCTTCACAGGCTTCAGTTATGTTAGGCCGGTCTCCTCTTTTCTCCAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006417 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008285 GO:0009266 GO:0009408 GO:0009409 GO:0009628 GO:0009651 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010608 GO:0010628 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032268 GO:0032270 GO:0034248 GO:0034250 GO:0035556 GO:0036211 GO:0042127 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045727 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051716 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0140096 GO:1901564 GO:2000112
KEGG Pathways
Metabolic & Signaling
ko01521 ko01522 ko04012 ko04066 ko04140 ko04150 ko04151 ko04152 ko04211 ko04212 ko04213 ko04350 ko04371 ko04666 ko04714 ko04910 ko04931 ko05165 ko05200 ko05205 ko05210 ko05212 ko05221 ko05224 ko05225 ko05226 ko05231 map01521 map01522 map04012 map04066 map04140 map04150 map04151 map04152 map04211 map04212 map04213 map04350 map04371 map04666 map04714 map04910 map04931 map05165 map05200 map05205 map05210 map05212 map05221 map05224 map05225 map05226 map05231
Pfam Domains
Protein Families
Pkinase Pkinase_C

Protein Analysis

479

Amino Acids

53.71

Weight (kDa)

6.2

Isoelectric Point (pI)

36.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 154 - 409 9.9e-77 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 155 - 392 1.7e-35 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 228 - 309 1.3e-06 ABC1 atypical kinase-like domain
Kinase-like PF14531 259 - 392 1.1e-09 Kinase-like
Pkinase_fungal PF17667 261 - 333 8.7e-07 Fungal protein kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1336
AciI CCGC 2 cut(s) 791, 1359
AclWI GGATC 4 cut(s) 215, 837, 1157, 1170
AcoI YGGCCR 1 cut(s) 865
AcsI RAATTY 2 cut(s) 368, 1071
AcuI CTGAAG 2 cut(s) 141, 1381
AfeI AGCGCT 1 cut(s) 1149
AfiI CCNNNNNNNGG 3 cut(s) 386, 1055, 1391
AflIII ACRYGT 2 cut(s) 39, 1335
AgsI TTSAA 8 cut(s) 194, 289, 410, 455, 472, 908, 1144, 1228
AjnI CCWGG 1 cut(s) 19
AjuI GAANNNNNNNTTGG 6 cut(s) 369, 401, 954, 986, 1260, 1292
AluBI AGCT 7 cut(s) 16, 115, 292, 602, 727, 757, 1275
AluI AGCT 7 cut(s) 16, 115, 292, 602, 727, 757, 1275
Alw26I GTCTC 2 cut(s) 348, 1420
AlwI GGATC 4 cut(s) 215, 837, 1157, 1170
Ama87I CYCGRG 1 cut(s) 110
Aor51HI AGCGCT 1 cut(s) 1149
AoxI GGCC 7 cut(s) 33, 136, 259, 739, 865, 1187, 1409
ApeKI GCWGC 6 cut(s) 92, 773, 992, 1150, 1153, 1373
ApoI RAATTY 2 cut(s) 368, 1071
Asp700I GAANNNNTTC 1 cut(s) 966
AspLEI GCGC 3 cut(s) 1150, 1176, 1215
AspS9I GGNCC 5 cut(s) 34, 137, 260, 1187, 1329
AsuC2I CCSGG 1 cut(s) 1294
AsuHPI GGTGA 5 cut(s) 98, 523, 704, 725, 791
AsuNHI GCTAGC 1 cut(s) 1361
AvaI CYCGRG 1 cut(s) 110
AvaII GGWCC 1 cut(s) 1329
BalI TGGCCA 1 cut(s) 867
BamHI GGATCC 1 cut(s) 1162
BbvI GCAGC 6 cut(s) 79, 760, 979, 1137, 1140, 1360
BccI CCATC 2 cut(s) 368, 857
BciT130I CCWGG 1 cut(s) 21
BclI TGATCA 1 cut(s) 628
BcnI CCSGG 1 cut(s) 1294
BcoDI GTCTC 2 cut(s) 348, 1420
BfaI CTAG 1 cut(s) 1362
BfmI CTRYAG 3 cut(s) 93, 993, 1154
BfoI RGCGCY 3 cut(s) 1151, 1177, 1216
BglII AGATCT 1 cut(s) 920
BisI GCNGC 7 cut(s) 93, 774, 993, 1151, 1154, 1359, 1374
BlpI GCTNAGC 1 cut(s) 293
BlsI GCNGC 7 cut(s) 94, 775, 994, 1152, 1155, 1360, 1375
Bme1390I CCNGG 2 cut(s) 21, 1294
Bme18I GGWCC 1 cut(s) 1329
BmeT110I CYCGRG 1 cut(s) 110
BmgT120I GGNCC 5 cut(s) 34, 137, 260, 1187, 1329
BmiI GGNNCC 4 cut(s) 262, 378, 940, 1164
BmrFI CCNGG 2 cut(s) 21, 1294
BmrI ACTGGG 2 cut(s) 55, 1065
BmsI GCATC 5 cut(s) 79, 187, 847, 1020, 1314
BmtI GCTAGC 1 cut(s) 1365
BmuI ACTGGG 2 cut(s) 55, 1065
BpmI CTGGAG 2 cut(s) 1271, 1412
Bpu1102I GCTNAGC 1 cut(s) 293
BpuMI CCSGG 1 cut(s) 1294
BsaI GGTCTC 1 cut(s) 1420
BsaJI CCNNGG 4 cut(s) 30, 75, 736, 1177
Bsc4I CCNNNNNNNGG 3 cut(s) 386, 1055, 1391
Bse118I RCCGGY 1 cut(s) 1411
Bse1I ACTGG 9 cut(s) 61, 146, 224, 339, 1004, 1042, 1060, 1244, 1254
Bse3DI GCAATG 1 cut(s) 549
BseBI CCWGG 1 cut(s) 21
BseDI CCNNGG 4 cut(s) 30, 75, 736, 1177
BseGI GGATG 3 cut(s) 94, 631, 862
BseLI CCNNNNNNNGG 3 cut(s) 386, 1055, 1391
BseMI GCAATG 1 cut(s) 549
BseMII CTCAG 4 cut(s) 26, 307, 594, 768
BseNI ACTGG 9 cut(s) 61, 146, 224, 339, 1004, 1042, 1060, 1244, 1254
BseRI GAGGAG 2 cut(s) 767, 1408
BseXI GCAGC 6 cut(s) 79, 760, 979, 1137, 1140, 1360
BseYI CCCAGC 2 cut(s) 757, 1354
Bsh1285I CGRYCG 1 cut(s) 1183
BshFI GGCC 7 cut(s) 35, 138, 261, 741, 867, 1189, 1411
BsiEI CGRYCG 1 cut(s) 1183
BsiHKCI CYCGRG 1 cut(s) 110
BsiSI CCGG 2 cut(s) 1293, 1412
BslFI GGGAC 3 cut(s) 538, 774, 993
BslI CCNNNNNNNGG 3 cut(s) 386, 1055, 1391
BsmAI GTCTC 2 cut(s) 348, 1420
BsmFI GGGAC 3 cut(s) 538, 774, 993
BsmI GAATGC 1 cut(s) 1375
BsnI GGCC 7 cut(s) 35, 138, 261, 741, 867, 1189, 1411
Bso31I GGTCTC 1 cut(s) 1420
BsoBI CYCGRG 1 cut(s) 110
Bsp1720I GCTNAGC 1 cut(s) 293
Bsp19I CCATGG 2 cut(s) 75, 736
BspACI CCGC 2 cut(s) 791, 1359
BspANI GGCC 7 cut(s) 35, 138, 261, 741, 867, 1189, 1411
BspCNI CTCAG 4 cut(s) 25, 306, 595, 769
BspLI GGNNCC 4 cut(s) 262, 378, 940, 1164
BspMAI CTGCAG 3 cut(s) 97, 997, 1158
BspOI GCTAGC 1 cut(s) 1365
BspPI GGATC 4 cut(s) 215, 837, 1157, 1170
BspTNI GGTCTC 1 cut(s) 1420
BsrDI GCAATG 1 cut(s) 549
BsrFI RCCGGY 1 cut(s) 1411
BsrI ACTGG 9 cut(s) 61, 146, 224, 339, 1004, 1042, 1060, 1244, 1254
BssAI RCCGGY 1 cut(s) 1411
BssECI CCNNGG 4 cut(s) 30, 75, 736, 1177
BssT1I CCWWGG 3 cut(s) 30, 75, 736
Bst2UI CCWGG 1 cut(s) 21
Bst4CI ACNGT 3 cut(s) 85, 943, 1140
Bst6I CTCTTC 2 cut(s) 722, 903
BstC8I GCNNGC 1 cut(s) 1363
BstDEI CTNAG 5 cut(s) 12, 293, 603, 650, 777
BstDSI CCRYGG 2 cut(s) 75, 736
BstEII GGTNACC 1 cut(s) 710
BstF5I GGATG 3 cut(s) 94, 631, 862
BstH2I RGCGCY 3 cut(s) 1151, 1177, 1216
BstHHI GCGC 3 cut(s) 1150, 1176, 1215
BstMAI GTCTC 2 cut(s) 348, 1420
BstMCI CGRYCG 1 cut(s) 1183
BstMWI GCNNNNNNNGC 1 cut(s) 182
BstNI CCWGG 1 cut(s) 21
BstNSI RCATGY 3 cut(s) 43, 955, 1339
BstPI GGTNACC 1 cut(s) 710
BstSCI CCNGG 2 cut(s) 19, 1292
BstSFI CTRYAG 3 cut(s) 93, 993, 1154
BstV1I GCAGC 6 cut(s) 79, 760, 979, 1137, 1140, 1360
BstX2I RGATCY 3 cut(s) 829, 920, 1162
BstXI CCANNNNNNTGG 2 cut(s) 812, 1239
BstYI RGATCY 3 cut(s) 829, 920, 1162
BsuRI GGCC 7 cut(s) 35, 138, 261, 741, 867, 1189, 1411
BtgI CCRYGG 2 cut(s) 75, 736
BtgZI GCGATG 1 cut(s) 1291
BtsCI GGATG 3 cut(s) 94, 631, 862
BtsI GCAGTG 1 cut(s) 1300
BtsIMutI CAGTG 5 cut(s) 90, 231, 333, 1136, 1300
Cac8I GCNNGC 1 cut(s) 1363
CfoI GCGC 3 cut(s) 1150, 1176, 1215
Cfr10I RCCGGY 1 cut(s) 1411
Cfr13I GGNCC 5 cut(s) 34, 137, 260, 1187, 1329
CsiI ACCWGGT 1 cut(s) 19
DdeI CTNAG 5 cut(s) 12, 293, 603, 650, 777
DrdI GACNNNNNNGTC 1 cut(s) 1336
DseDI GACNNNNNNGTC 1 cut(s) 1336
EaeI YGGCCR 1 cut(s) 865
Eam1104I CTCTTC 2 cut(s) 722, 903
EarI CTCTTC 2 cut(s) 722, 903
Eco130I CCWWGG 3 cut(s) 30, 75, 736
Eco31I GGTCTC 1 cut(s) 1420
Eco47I GGWCC 1 cut(s) 1329
Eco47III AGCGCT 1 cut(s) 1149
Eco57I CTGAAG 2 cut(s) 141, 1381
Eco88I CYCGRG 1 cut(s) 110
Eco91I GGTNACC 1 cut(s) 710
EcoO65I GGTNACC 1 cut(s) 710
EcoRII CCWGG 1 cut(s) 19
EcoT14I CCWWGG 3 cut(s) 30, 75, 736
EcoT22I ATGCAT 1 cut(s) 825
ErhI CCWWGG 3 cut(s) 30, 75, 736
FaqI GGGAC 3 cut(s) 538, 774, 993
FbaI TGATCA 1 cut(s) 628
Fnu4HI GCNGC 7 cut(s) 93, 774, 993, 1151, 1154, 1359, 1374
FokI GGATG 3 cut(s) 101, 618, 869
Fsp4HI GCNGC 7 cut(s) 93, 774, 993, 1151, 1154, 1359, 1374
FspBI CTAG 1 cut(s) 1362
GlaI GCGC 3 cut(s) 1149, 1175, 1214
GluI GCNGC 7 cut(s) 93, 774, 993, 1151, 1154, 1359, 1374
GsaI CCCAGC 2 cut(s) 761, 1358
GsuI CTGGAG 2 cut(s) 1271, 1412
HaeII RGCGCY 3 cut(s) 1151, 1177, 1216
HaeIII GGCC 7 cut(s) 35, 138, 261, 741, 867, 1189, 1411
HapII CCGG 2 cut(s) 1293, 1412
HhaI GCGC 3 cut(s) 1150, 1176, 1215
Hin6I GCGC 3 cut(s) 1148, 1174, 1213
HinP1I GCGC 3 cut(s) 1148, 1174, 1213
HincII GTYRAC 1 cut(s) 879
HindII GTYRAC 1 cut(s) 879
HindIII AAGCTT 1 cut(s) 1273
HinfI GANTC 4 cut(s) 323, 580, 1014, 1349
HpaII CCGG 2 cut(s) 1293, 1412
HphI GGTGA 5 cut(s) 98, 523, 704, 725, 791
Hpy166II GTNNAC 2 cut(s) 277, 879
Hpy188I TCNGA 4 cut(s) 57, 103, 532, 666
Hpy188III TCNNGA 4 cut(s) 374, 407, 439, 1429
Hpy8I GTNNAC 2 cut(s) 277, 879
HpyAV CCTTC 5 cut(s) 514, 541, 1119, 1153, 1396
HpyCH4III ACNGT 3 cut(s) 85, 943, 1140
HpyF10VI GCNNNNNNNGC 1 cut(s) 182
HpyF3I CTNAG 5 cut(s) 12, 293, 603, 650, 777
HspAI GCGC 3 cut(s) 1148, 1174, 1213
Ksp22I TGATCA 1 cut(s) 628
LmnI GCTCC 1 cut(s) 754
Lsp1109I GCAGC 6 cut(s) 79, 760, 979, 1137, 1140, 1360
LweI GCATC 5 cut(s) 79, 187, 847, 1020, 1314
MabI ACCWGGT 1 cut(s) 19
MaeI CTAG 1 cut(s) 1362
MaeIII GTNAC 1 cut(s) 710
MboII GAAGA 9 cut(s) 206, 332, 445, 467, 529, 589, 709, 920, 1093
MflI RGATCY 3 cut(s) 829, 920, 1162
MlsI TGGCCA 1 cut(s) 867
MluCI AATT 7 cut(s) 51, 178, 368, 624, 925, 1071, 1312
MluNI TGGCCA 1 cut(s) 867
MlyI GAGTC 1 cut(s) 1023
MmeI TCCRAC 3 cut(s) 670, 1190, 1291
Mox20I TGGCCA 1 cut(s) 867
Mph1103I ATGCAT 1 cut(s) 825
MroXI GAANNNNTTC 1 cut(s) 966
MscI TGGCCA 1 cut(s) 867
MseI TTAA 5 cut(s) 26, 617, 702, 834, 1089
MslI CAYNNNNRTG 2 cut(s) 804, 810
Msp20I TGGCCA 1 cut(s) 867
MspI CCGG 2 cut(s) 1293, 1412
MspR9I CCNGG 2 cut(s) 21, 1294
Mva1269I GAATGC 1 cut(s) 1375
MvaI CCWGG 1 cut(s) 21
MwoI GCNNNNNNNGC 1 cut(s) 182
NciI CCSGG 1 cut(s) 1294
NcoI CCATGG 2 cut(s) 75, 736
NheI GCTAGC 1 cut(s) 1361
NlaIV GGNNCC 4 cut(s) 262, 378, 940, 1164
NmeAIII GCCGAG 1 cut(s) 1237
NmuCI GTSAC 1 cut(s) 710
NsiI ATGCAT 1 cut(s) 825
NspI RCATGY 3 cut(s) 43, 955, 1339
PaeR7I CTCGAG 1 cut(s) 110
PciI ACATGT 2 cut(s) 39, 1335
PctI GAATGC 1 cut(s) 1375
PdmI GAANNNNTTC 1 cut(s) 966
PfeI GAWTC 3 cut(s) 323, 580, 1349
PkrI GCNGC 7 cut(s) 94, 775, 994, 1152, 1155, 1360, 1375
PleI GAGTC 1 cut(s) 1022
PpsI GAGTC 1 cut(s) 1022
PscI ACATGT 2 cut(s) 39, 1335
Psp6I CCWGG 1 cut(s) 19
PspEI GGTNACC 1 cut(s) 710
PspFI CCCAGC 2 cut(s) 757, 1354
PspGI CCWGG 1 cut(s) 19
PspN4I GGNNCC 4 cut(s) 262, 378, 940, 1164
PspPI GGNCC 5 cut(s) 34, 137, 260, 1187, 1329
PspXI VCTCGAGB 1 cut(s) 110
PstI CTGCAG 3 cut(s) 97, 997, 1158
PsuI RGATCY 3 cut(s) 829, 920, 1162
RseI CAYNNNNRTG 2 cut(s) 804, 810
SaqAI TTAA 5 cut(s) 26, 617, 702, 834, 1089
SatI GCNGC 7 cut(s) 93, 774, 993, 1151, 1154, 1359, 1374
Sau96I GGNCC 5 cut(s) 34, 137, 260, 1187, 1329
SchI GAGTC 1 cut(s) 1023
ScrFI CCNGG 2 cut(s) 21, 1294
SexAI ACCWGGT 1 cut(s) 19
SfaNI GCATC 5 cut(s) 79, 187, 847, 1020, 1314
SfcI CTRYAG 3 cut(s) 93, 993, 1154
Sfr274I CTCGAG 1 cut(s) 110
SinI GGWCC 1 cut(s) 1329
SlaI CTCGAG 1 cut(s) 110
SmiMI CAYNNNNRTG 2 cut(s) 804, 810
SmlI CTYRAG 1 cut(s) 110
SmoI CTYRAG 1 cut(s) 110
Sse9I AATT 7 cut(s) 51, 178, 368, 624, 925, 1071, 1312
SsiI CCGC 2 cut(s) 791, 1359
SspMI CTAG 1 cut(s) 1362
StyD4I CCNGG 2 cut(s) 19, 1292
StyI CCWWGG 3 cut(s) 30, 75, 736
TaaI ACNGT 3 cut(s) 85, 943, 1140
TaqI TCGA 4 cut(s) 111, 188, 393, 438
TaqII GACCGA 2 cut(s) 1169, 1346
TasI AATT 7 cut(s) 51, 178, 368, 624, 925, 1071, 1312
TauI GCSGC 1 cut(s) 1361
TfiI GAWTC 3 cut(s) 323, 580, 1349
Tru1I TTAA 5 cut(s) 26, 617, 702, 834, 1089
Tru9I TTAA 5 cut(s) 26, 617, 702, 834, 1089
TscAI CASTG 5 cut(s) 90, 231, 333, 1143, 1307
TseFI GTSAC 1 cut(s) 710
TseI GCWGC 6 cut(s) 92, 773, 992, 1150, 1153, 1373
Tsp45I GTSAC 1 cut(s) 710
TspDTI ATGAA 6 cut(s) 411, 435, 446, 560, 611, 628
TspRI CASTG 5 cut(s) 90, 231, 333, 1143, 1307
VpaK11BI GGWCC 1 cut(s) 1329
XapI RAATTY 2 cut(s) 368, 1071
XceI RCATGY 3 cut(s) 43, 955, 1339
XcmI CCANNNNNNNNNTGG 1 cut(s) 82
XhoI CTCGAG 1 cut(s) 110
XmnI GAANNNNTTC 1 cut(s) 966
XspI CTAG 1 cut(s) 1362
Zsp2I ATGCAT 1 cut(s) 825
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.