Rorug03G0041300

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0005471
Physical Location & Seq
Forward (+)
3210859 .. 3219999
9141 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0041300.1

Sequence Viewer

Length: 3621 bp
ATGGAGGCGAGCAACAATAGCAGCAGCAACCTAGCTGGCAGCGTTGCCCAAGCCATTGCCGTCTCTCTCGACTGGAGCTCCACTCCCGACGCGCGCAAAGCCGCCGTGGCTTTTCTTGAATCGATTAAAACTGGAGATGTGCGTGTTTTGGCAAATACAGCGTTCCTTCTAGTGAAAAAGGATTGGTCTTCTGAAATTCGGTTGCATGCATTTAAAATGTTGCAGCATTTGGTCCGGTTGCGGTGGGAGGAACTAAACCCTACAGAACATAGAAACTTTGCTAATGTCACTGTTGATCTAATGTCTGAAATTGCAAATCCTAATGAAGAGTGGGCTTTGAAAAGTCAGACAGCAGCACTTGTTGCTGAGATAGTTAGAAGTGAAGGAGTAGATCTCTGGCAGGAACTGCTTCCAACTCTGGTTTCCTTATCTGCAAAGGGTCCTATACAAGCTGAGTTGGTCTCCATGATGCTGAGGTGGCTTCCTGAAGACATTACTGTTCACAATGAAGATTTGGAAGCTGATCGGAGAAGGCTATTGTTGCGTGGGCTGACCCTATCCTTACCTGAAATTTTGCCTCTATTATACAACTTACTAGAGAGGCATTTTGGAGCTGCCATGAGTGAAGCCGGGAAACAACAAGTGGACCTTGCAAAACAACATGCATCTGCAGTAACAGCTACTTTAAATGCTGTTAATGCATATTCTGAATGGGCTCCATTGCCTGATCTTGCTAAATATGGTATAATTCATGGGTGTGGTTTCTTACTTTCTTCACCTGACTTCCGTCTTCATGCTTGTGAGTTTTTCAAACTTGTTTCCCCAAGAAAGAGACATGTTGATACATCGACTCCTGAATTTGATTCTGCAATGAGTACTGTCTTTCATATTTTAATGAATGCCTCCAAAGAATTTTTGTACAGCTCTGGCGTCAATGCTGGGGTGATTGATGAAAGCAATATTGAATTTGCGGAATATTTATGTGAAAGTATGGTGTCTTTGGGTTCAACAAACTTGCAATGTATCGTGGGTGATAGCACCACATTACCTCTTTATTTACAACAGATGCTCGGGTTTTTTCAACATCATAAGCTAGTTCTTCATTTTCAATCTCTCCATTTTTGGCTGGCACTTATGAGAGACTTAATGTCTAAGCCACCTAAGTCAAAGGTGGCTGCACACTCATCAGCTGATGGTTCTGACCAGGCTGATAAGGAAAAGCAGAAGATTTTGAGTTTTGTGAACGATGACATATGTAGTGCACTTCTGGATGTATCTTTCCAACGATTGCTCAAGAGAGAAAAGATTCTTCATGGAACATCAATCTCTTTGGGGTCATTGGAGTTATGGAGTGATGATGTTGACAGCAAAGGAACTTTCGGCCAGTATCGCTCAAAGCTGTTAGAACTGATCAAGTTTGTTACCTCATACAAGCCTGTTATAGCTGGTGCTAAGGTTTCTGAAAGAATTGATACAATCATTAAAAGCCTCTTGCTTTCTCCAATGCCTGCTCAGGACTTAGCTGTGATGGAAAGCATGCAGTCGGCTCTTGAAAATGTTGTAAGCACTATTTTTGATAGATCAAATGAAATTGGTGGGGGCCATTCTGAAGTTCAACTAGGACTATGCAGAATATTCGAAGGTTTGCTTCAGCAACTTCTCTCTTTGAAGTGGACTGAGCCAGTCCTTGTGGAAGTACTTGGTCACTACTTGGATGCAATGGGTCCATTTTTGAACTATTTCCCAGATGCAGCAGGCAGCGTCATCAATAAACTATTTGAGCTCCTAAACTCACTCCCTTTTGCTGTTAAGGATCCATCTACAAGTAGTGCACGATATGCAAGGTTGCAAATTTGTACATCGTTTATTCGTGTAGCAAAAACTGCTGACAAAAGTATTCTTCCTCACATGAAGGGTATAGCTGATACCATGGCATATTTGCAAAAGGAAGGTCGTTTACTTCGTGGTGAACATAATCTTCTAGGCGAAGCATTTCTTGTCATGGCTTCTGCTGCTGGATTTCAACAGCAGCAAGAAGTTTTGGCCTGGTTACTAGAACCCTTGAGCCAGCAATGGGTACAGATAGAGTGGCAAAATAATTATTTATCTGAACCACTGGGTCTTGTTCGTTTGTGCATGGAGACCCCAACTATGTGGTCAGTTTTCCACTGTATCACATTCTTTGAGAAGGCACTTAAGAGAAGTGGAACCCGGAAAACACATTTGAGTTCACAAAACAACTCAGCCGCAAGTTCTACTCCCTTGCATCCTATGGCTTCTCATCTATCATGGATGCTGCCACCTCTTCCCAAACTTCTTCGTGCCATACATTCCCTTTGGTCTCCATCTGTATCTCAAACTCTACCTGGAGAAATGAAAGCTGCAATGACCATGAGCGATGTTGAGCAATACAGTCTTCTTGGTGAAGGAAACCCTAAATTGTCAAAGGGTACATTAACCTCTGGTCCTGGATCTCTTGTCACCATGAGTAAGGAAGGATATACAGAGCCAAATGAATCGGATATACGAAATTGGATGAAAGGGATCAGAGACAGTGGGTACAATGTATTGGGCCTGGCAACCACTGTCGGGGACACATTCTACAAATGTTTGGACAGTCATTCTGTTGCTCTAGCTTTGGTAGAGAATATACAGTCAATGGAATTCAGGCATATAAGATTGCTTGTTCATTCAGTTTTGATTCCTTTGGTTAAAAATTGCCCTGTGGATTTGTGGGAGGTATGGCTGGAAAAGCTCTTGCACCCATTATTTCTACACTCCCATCAGGCTCTTAGTTGCTCCTGGTCCAGTCTTCTACAAGAAGGTAGAGCAAAGGTTCCGGATGTCCATGCCATACTTGCTGGGACAGACTTAAAAGTGGAAGTAATGGAAGAAAAGCTACTAAGAGATCTTACTCGAGAGATTTGTTCACTCCTCTCTGCCATTGCTTTGCCACAGCTAAATACTGGCCTTCCATCCTTGGAGCACTCTGGGCAAATCAGCCGAGTCGATCTATCTTCACTCAAGTCTTTAGATTCTTTTGCATCGAGCTCTATGGTTAGTTTCATTTTGAAGCACCAAGGTCTTGCCCTTCCAGCACTGCAGATATGTTTAGAAGCTTTTACATGGACAGATGGTGATGCCATGACAAAAGTTTCTTCCTTTTGTGGTGCTTTGGTTGTTCTAGCTATATTCACAAATAGTGTGGAACTCCAACAGTTTGTTTGTAAGGATCTCTTTTCTGCAATTATCCAAGGTTTAGCCCTTGAATCAAATGCTTTCATCAGTGCTGATTTGGTTGGTCACTGCCGTGACATTTACATATATCTTTGTGATAGACATCCCGCTCCTAGGCAGATTTTGCTCTCACTTCCCTGCATCAAACAGCATGATTTGCTTGCTTTTGAAGAAGCTTTGACGAAGACAGCTAGTCCTAAAGAGCAAAAACAATATATGAAAAGCCTGCTATTATTAGCTACCGGAAACAAGTTAAAAGCACTTGTTGCTCAGAAAACTGTAAATGTTATAACAAATGTATCAACGAAGCCTCGCAACACGGTCAACGTTGCAGAAACCAGAGTTGATGAGGGGGAAGTTATTGGACTGGCAGCCCTATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006417 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008285 GO:0009266 GO:0009408 GO:0009409 GO:0009628 GO:0009651 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010608 GO:0010628 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032268 GO:0032270 GO:0034248 GO:0034250 GO:0035556 GO:0036211 GO:0042127 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045727 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051716 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0140096 GO:1901564 GO:2000112
KEGG Pathways
Metabolic & Signaling
ko01521 ko01522 ko04012 ko04066 ko04140 ko04150 ko04151 ko04152 ko04211 ko04212 ko04213 ko04350 ko04371 ko04666 ko04714 ko04910 ko04931 ko05165 ko05200 ko05205 ko05210 ko05212 ko05221 ko05224 ko05225 ko05226 ko05231 map01521 map01522 map04012 map04066 map04140 map04150 map04151 map04152 map04211 map04212 map04213 map04350 map04371 map04666 map04714 map04910 map04931 map05165 map05200 map05205 map05210 map05212 map05221 map05224 map05225 map05226 map05231
Pfam Domains
Protein Families
Pkinase Pkinase_C

Protein Analysis

1206

Amino Acids

133.43

Weight (kDa)

6.06

Isoelectric Point (pI)

41.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Xpo1 PF08389 111 - 269 4.3e-25 Exportin 1-like protein
Exportin-5 PF19273 318 - 1171 0.0 Exportin-5 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3527
AccBSI CCGCTC 1 cut(s) 3347
AccII CGCG 2 cut(s) 92, 94
AccIII TCCGGA 1 cut(s) 2839
AciI CCGC 5 cut(s) 102, 241, 971, 2247, 3345
AclI AACGTT 1 cut(s) 3564
AclWI GGATC 5 cut(s) 1808, 1821, 2479, 2552, 3240
AcoI YGGCCR 1 cut(s) 1381
AcsI RAATTY 7 cut(s) 195, 570, 857, 911, 965, 1851, 2663
AcuI CTGAAG 3 cut(s) 507, 1629, 1634
AcyI GRCGYC 1 cut(s) 930
AfaI GTAC 7 cut(s) 877, 920, 1698, 1858, 2079, 2452, 2561
AfiI CCNNNNNNNGG 3 cut(s) 2074, 2589, 3351
AflII CTTAAG 1 cut(s) 2195
AflIII ACRYGT 1 cut(s) 835
AhdI GACNNNNNGTC 2 cut(s) 1147, 3429
AjnI CCWGG 6 cut(s) 1203, 2045, 2365, 2467, 2574, 2801
AjuI GAANNNNNNNTTGG 2 cut(s) 406, 438
AleI CACNNNNGTG 1 cut(s) 3309
Alw21I GWGCWC 6 cut(s) 80, 1264, 1785, 1834, 2988, 3053
Alw26I GTCTC 7 cut(s) 67, 466, 826, 1134, 2135, 2346, 2544
Alw44I GTGCAC 2 cut(s) 1260, 1830
AlwI GGATC 5 cut(s) 1808, 1821, 2479, 2552, 3240
AlwNI CAGNNNCTG 1 cut(s) 406
Ama87I CYCGRG 2 cut(s) 1070, 2916
Aor13HI TCCGGA 1 cut(s) 2839
AoxI GGCC 5 cut(s) 1381, 1600, 2043, 2572, 2968
ApaLI GTGCAC 2 cut(s) 1260, 1830
ApoI RAATTY 7 cut(s) 195, 570, 857, 911, 965, 1851, 2663
ArsI GACNNNNNNTTYG 2 cut(s) 1936, 1968
Asp700I GAANNNNTTC 4 cut(s) 408, 1305, 1739, 1992
AspA2I CCTAGG 1 cut(s) 3350
AspLEI GCGC 2 cut(s) 94, 96
AspS9I GGNCC 8 cut(s) 232, 440, 646, 1600, 1724, 2465, 2572, 2805
AsuC2I CCSGG 2 cut(s) 631, 2212
AsuHPI GGTGA 7 cut(s) 768, 955, 1043, 1979, 2435, 2473, 3149
AsuII TTCGAA 1 cut(s) 1638
AvaI CYCGRG 2 cut(s) 1070, 2916
AvaII GGWCC 6 cut(s) 232, 440, 646, 1724, 2465, 2805
AvrII CCTAGG 1 cut(s) 3350
BaeGI GKGCMC 2 cut(s) 1264, 1834
BaeI ACNNNNGTAYC 2 cut(s) 3519, 3552
BamHI GGATCC 1 cut(s) 1813
BanII GRGCYC 4 cut(s) 80, 718, 1785, 3053
BarI GAAGNNNNNNTAC 4 cut(s) 1941, 1973, 2883, 2915
BbsI GAAGAC 6 cut(s) 180, 495, 782, 2408, 2804, 3428
Bbv12I GWGCWC 6 cut(s) 80, 1264, 1785, 1834, 2988, 3053
BbvCI CCTCAGC 1 cut(s) 473
BccI CCATC 7 cut(s) 1187, 1522, 1825, 2353, 2790, 2983, 3128
BceAI ACGGC 3 cut(s) 44, 89, 3294
BcgI CGANNNNNNTGC 2 cut(s) 1618, 1652
BciT130I CCWGG 6 cut(s) 1205, 2047, 2367, 2469, 2576, 2803
BclI TGATCA 1 cut(s) 1410
BcnI CCSGG 2 cut(s) 631, 2212
BcoDI GTCTC 7 cut(s) 67, 466, 826, 1134, 2135, 2346, 2544
BfmI CTRYAG 3 cut(s) 261, 669, 3101
BfrI CTTAAG 1 cut(s) 2195
BglI GCCNNNNNGGC 1 cut(s) 107
BglII AGATCT 2 cut(s) 391, 2908
BlnI CCTAGG 1 cut(s) 3350
BmcAI AGTACT 2 cut(s) 877, 1698
Bme1390I CCNGG 8 cut(s) 631, 1205, 2047, 2212, 2367, 2469, 2576, 2803
Bme18I GGWCC 6 cut(s) 232, 440, 646, 1724, 2465, 2805
BmeRI GACNNNNNGTC 2 cut(s) 1147, 3429
BmeT110I CYCGRG 2 cut(s) 1070, 2916
BmgT120I GGNCC 8 cut(s) 232, 440, 646, 1600, 1724, 2465, 2572, 2805
BmiI GGNNCC 7 cut(s) 441, 717, 1601, 1725, 1815, 2209, 2838
BmrFI CCNGG 8 cut(s) 631, 1205, 2047, 2212, 2367, 2469, 2576, 2803
BmrI ACTGGG 1 cut(s) 2126
BmuI ACTGGG 1 cut(s) 2126
BoxI GACNNNNGTC 1 cut(s) 786
BpiI GAAGAC 6 cut(s) 180, 495, 782, 2408, 2804, 3428
BplI GAGNNNNNCTC 6 cut(s) 67, 99, 378, 410, 446, 478
BpmI CTGGAG 3 cut(s) 94, 153, 2388
Bpu10I CCTNAGC 3 cut(s) 473, 1452, 1512
Bpu14I TTCGAA 1 cut(s) 1638
BpuEI CTTGAG 3 cut(s) 1277, 2083, 3008
BpuMI CCSGG 2 cut(s) 631, 2212
Bsa29I ATCGAT 1 cut(s) 122
BsaBI GATNNNNATC 1 cut(s) 3339
BsaHI GRCGYC 1 cut(s) 930
BsaI GGTCTC 3 cut(s) 466, 2135, 2346
BsaJI CCNNGG 6 cut(s) 105, 1929, 2979, 3079, 3253, 3350
BsaWI WCCGGW 3 cut(s) 234, 2839, 3479
BsaXI ACNNNNNCTCC 4 cut(s) 62, 92, 835, 865
Bsc4I CCNNNNNNNGG 3 cut(s) 2074, 2589, 3351
Bse1I ACTGG 8 cut(s) 77, 136, 1384, 1682, 2121, 2808, 2971, 3609
Bse3DI GCAATG 8 cut(s) 54, 719, 876, 1025, 1725, 2078, 2391, 2943
Bse8I GATNNNNATC 1 cut(s) 3339
BseAI TCCGGA 1 cut(s) 2839
BseBI CCWGG 6 cut(s) 1205, 2047, 2367, 2469, 2576, 2803
BseCI ATCGAT 1 cut(s) 122
BseDI CCNNGG 6 cut(s) 105, 1929, 2979, 3079, 3253, 3350
BseGI GGATG 8 cut(s) 1276, 1720, 2266, 2298, 2541, 2848, 2975, 3340
BseJI GATNNNNATC 1 cut(s) 3339
BseLI CCNNNNNNNGG 3 cut(s) 2074, 2589, 3351
BseMI GCAATG 8 cut(s) 54, 719, 876, 1025, 1725, 2078, 2391, 2943
BseMII CTCAG 7 cut(s) 357, 444, 464, 1526, 1668, 2256, 3521
BseNI ACTGG 8 cut(s) 77, 136, 1384, 1682, 2121, 2808, 2971, 3609
BsePI GCGCGC 1 cut(s) 92
BseRI GAGGAG 1 cut(s) 2924
BseSI GKGCMC 2 cut(s) 1264, 1834
BseYI CCCAGC 2 cut(s) 938, 2861
BsgI GTGCAG 1 cut(s) 1161
Bsh1236I CGCG 2 cut(s) 92, 94
BshFI GGCC 5 cut(s) 1383, 1602, 2045, 2574, 2970
BshVI ATCGAT 1 cut(s) 122
BsiHKAI GWGCWC 6 cut(s) 80, 1264, 1785, 1834, 2988, 3053
BsiHKCI CYCGRG 2 cut(s) 1070, 2916
BsiSI CCGG 5 cut(s) 235, 630, 2212, 2840, 3480
BslFI GGGAC 2 cut(s) 2606, 2878
BslI CCNNNNNNNGG 3 cut(s) 2074, 2589, 3351
BsmAI GTCTC 7 cut(s) 67, 466, 826, 1134, 2135, 2346, 2544
BsmBI CGTCTC 1 cut(s) 67
BsmFI GGGAC 2 cut(s) 2606, 2878
BsmI GAATGC 1 cut(s) 904
BsnI GGCC 5 cut(s) 1383, 1602, 2045, 2574, 2970
Bso31I GGTCTC 3 cut(s) 466, 2135, 2346
BsoBI CYCGRG 2 cut(s) 1070, 2916
Bsp119I TTCGAA 1 cut(s) 1638
Bsp1286I GDGCHC 7 cut(s) 80, 718, 1264, 1785, 1834, 2988, 3053
Bsp13I TCCGGA 1 cut(s) 2839
Bsp1407I TGTACA 2 cut(s) 918, 1856
Bsp19I CCATGG 1 cut(s) 1929
BspACI CCGC 5 cut(s) 102, 241, 971, 2247, 3345
BspANI GGCC 5 cut(s) 1383, 1602, 2045, 2574, 2970
BspCNI CTCAG 7 cut(s) 358, 445, 465, 1525, 1669, 2255, 3520
BspDI ATCGAT 1 cut(s) 122
BspEI TCCGGA 1 cut(s) 2839
BspFNI CGCG 2 cut(s) 92, 94
BspHI TCATGA 1 cut(s) 3617
BspLI GGNNCC 7 cut(s) 441, 717, 1601, 1725, 1815, 2209, 2838
BspMAI CTGCAG 2 cut(s) 673, 3105
BspPI GGATC 5 cut(s) 1808, 1821, 2479, 2552, 3240
BspT104I TTCGAA 1 cut(s) 1638
BspTI CTTAAG 1 cut(s) 2195
BspTNI GGTCTC 3 cut(s) 466, 2135, 2346
BsrBI CCGCTC 1 cut(s) 3347
BsrDI GCAATG 8 cut(s) 54, 719, 876, 1025, 1725, 2078, 2391, 2943
BsrGI TGTACA 2 cut(s) 918, 1856
BsrI ACTGG 8 cut(s) 77, 136, 1384, 1682, 2121, 2808, 2971, 3609
BssECI CCNNGG 6 cut(s) 105, 1929, 2979, 3079, 3253, 3350
BssHII GCGCGC 1 cut(s) 92
BssNI GRCGYC 1 cut(s) 930
BssT1I CCWWGG 5 cut(s) 1929, 2979, 3079, 3253, 3350
Bst2UI CCWGG 6 cut(s) 1205, 2047, 2367, 2469, 2576, 2803
Bst6I CTCTTC 2 cut(s) 321, 2310
BstACI GRCGYC 1 cut(s) 930
BstAFI CTTAAG 1 cut(s) 2195
BstAPI GCANNNNNTGC 7 cut(s) 362, 406, 1838, 1883, 3361, 3394, 3503
BstAUI TGTACA 2 cut(s) 918, 1856
BstBI TTCGAA 1 cut(s) 1638
BstDSI CCRYGG 2 cut(s) 105, 1929
BstF5I GGATG 8 cut(s) 1276, 1720, 2266, 2298, 2541, 2848, 2975, 3340
BstFNI CGCG 2 cut(s) 92, 94
BstHHI GCGC 2 cut(s) 94, 96
BstMAI GTCTC 7 cut(s) 67, 466, 826, 1134, 2135, 2346, 2544
BstNI CCWGG 6 cut(s) 1205, 2047, 2367, 2469, 2576, 2803
BstNSI RCATGY 4 cut(s) 209, 665, 839, 1540
BstPAI GACNNNNGTC 1 cut(s) 786
BstSCI CCNGG 8 cut(s) 629, 1203, 2045, 2210, 2365, 2467, 2574, 2801
BstSFI CTRYAG 3 cut(s) 261, 669, 3101
BstSLI GKGCMC 2 cut(s) 1264, 1834
BstUI CGCG 2 cut(s) 92, 94
BstV2I GAAGAC 6 cut(s) 180, 495, 782, 2408, 2804, 3428
BstX2I RGATCY 5 cut(s) 391, 1813, 2471, 2908, 3232
BstXI CCANNNNNNTGG 1 cut(s) 2154
BstYI RGATCY 5 cut(s) 391, 1813, 2471, 2908, 3232
Bsu15I ATCGAT 1 cut(s) 122
BsuRI GGCC 5 cut(s) 1383, 1602, 2045, 2574, 2970
BsuTUI ATCGAT 1 cut(s) 122
BtgI CCRYGG 2 cut(s) 105, 1929
BtgZI GCGATG 1 cut(s) 2412
BtsCI GGATG 8 cut(s) 1276, 1720, 2266, 2298, 2541, 2848, 2975, 3340
BtsI GCAGTG 2 cut(s) 3098, 3304
BtsIMutI CAGTG 8 cut(s) 288, 2114, 2167, 2560, 2583, 3098, 3292, 3304
CaiI CAGNNNCTG 1 cut(s) 406
CciI TCATGA 1 cut(s) 3617
CfoI GCGC 2 cut(s) 94, 96
Cfr13I GGNCC 8 cut(s) 232, 440, 646, 1600, 1724, 2465, 2572, 2805
ClaI ATCGAT 1 cut(s) 122
CseI GACGC 3 cut(s) 98, 919, 1750
Csp6I GTAC 7 cut(s) 876, 919, 1697, 1857, 2078, 2451, 2560
CspCI CAANNNNNGTGG 2 cut(s) 3186, 3221
CviQI GTAC 7 cut(s) 876, 919, 1697, 1857, 2078, 2451, 2560
DraI TTTAAA 2 cut(s) 214, 687
DriI GACNNNNNGTC 2 cut(s) 1147, 3429
EaeI YGGCCR 1 cut(s) 1381
Eam1104I CTCTTC 2 cut(s) 321, 2310
Eam1105I GACNNNNNGTC 2 cut(s) 1147, 3429
EarI CTCTTC 2 cut(s) 321, 2310
Ecl136II GAGCTC 3 cut(s) 78, 1783, 3051
Eco130I CCWWGG 5 cut(s) 1929, 2979, 3079, 3253, 3350
Eco24I GRGCYC 4 cut(s) 80, 718, 1785, 3053
Eco31I GGTCTC 3 cut(s) 466, 2135, 2346
Eco47I GGWCC 6 cut(s) 232, 440, 646, 1724, 2465, 2805
Eco53kI GAGCTC 3 cut(s) 78, 1783, 3051
Eco57I CTGAAG 3 cut(s) 507, 1629, 1634
Eco88I CYCGRG 2 cut(s) 1070, 2916
EcoICRI GAGCTC 3 cut(s) 78, 1783, 3051
EcoO109I RGGNCCY 1 cut(s) 440
EcoRI GAATTC 1 cut(s) 2663
EcoRII CCWGG 6 cut(s) 1203, 2045, 2365, 2467, 2574, 2801
EcoT14I CCWWGG 5 cut(s) 1929, 2979, 3079, 3253, 3350
EcoT22I ATGCAT 3 cut(s) 211, 667, 703
EcoT38I GRGCYC 4 cut(s) 80, 718, 1785, 3053
ErhI CCWWGG 5 cut(s) 1929, 2979, 3079, 3253, 3350
Esp3I CGTCTC 1 cut(s) 67
FalI AAGNNNNNCTT 8 cut(s) 633, 665, 1632, 1664, 1980, 2012, 3221, 3253
FaqI GGGAC 2 cut(s) 2606, 2878
FauI CCCGC 1 cut(s) 3352
FauNDI CATATG 1 cut(s) 1253
FbaI TGATCA 1 cut(s) 1410
FokI GGATG 8 cut(s) 1283, 1727, 2253, 2305, 2548, 2855, 2962, 3327
FriOI GRGCYC 4 cut(s) 80, 718, 1785, 3053
GlaI GCGC 2 cut(s) 93, 95
GsaI CCCAGC 2 cut(s) 942, 2865
GsuI CTGGAG 3 cut(s) 94, 153, 2388
HaeIII GGCC 5 cut(s) 1383, 1602, 2045, 2574, 2970
HapII CCGG 5 cut(s) 235, 630, 2212, 2840, 3480
HgaI GACGC 3 cut(s) 98, 919, 1750
HhaI GCGC 2 cut(s) 94, 96
Hin1I GRCGYC 1 cut(s) 930
Hin6I GCGC 2 cut(s) 92, 94
HinP1I GCGC 2 cut(s) 92, 94
HincII GTYRAC 2 cut(s) 1363, 3562
HindII GTYRAC 2 cut(s) 1363, 3562
HindIII AAGCTT 2 cut(s) 3117, 3411
HinfI GANTC 9 cut(s) 119, 850, 863, 1306, 2516, 2701, 3006, 3035, 3269
HpaII CCGG 5 cut(s) 235, 630, 2212, 2840, 3480
HphI GGTGA 7 cut(s) 768, 955, 1043, 1979, 2435, 2473, 3149
Hpy99I CGWCG 1 cut(s) 92
HpyCH4IV ACGT 1 cut(s) 3564
HpySE526I ACGT 1 cut(s) 3564
Hsp92I GRCGYC 1 cut(s) 930
HspAI GCGC 2 cut(s) 92, 94
Kpn2I TCCGGA 1 cut(s) 2839
Ksp22I TGATCA 1 cut(s) 1410
LmnI GCTCC 8 cut(s) 75, 83, 611, 721, 1788, 2804, 2983, 3352
MaeII ACGT 1 cut(s) 3564
MaeIII GTNAC 8 cut(s) 286, 673, 1420, 1703, 2049, 2479, 3302, 3311
MbiI CCGCTC 1 cut(s) 3347
MflI RGATCY 5 cut(s) 391, 1813, 2471, 2908, 3232
MhlI GDGCHC 7 cut(s) 80, 718, 1264, 1785, 1834, 2988, 3053
MlyI GAGTC 2 cut(s) 844, 3015
MmeI TCCRAC 3 cut(s) 437, 1306, 3238
Mph1103I ATGCAT 3 cut(s) 211, 667, 703
MroI TCCGGA 1 cut(s) 2839
MroXI GAANNNNTTC 4 cut(s) 408, 1305, 1739, 1992
MslI CAYNNNNRTG 3 cut(s) 756, 798, 3309
MspA1I CMGCKG 1 cut(s) 1190
MspCI CTTAAG 1 cut(s) 2195
MspI CCGG 5 cut(s) 235, 630, 2212, 2840, 3480
MspR9I CCNGG 8 cut(s) 631, 1205, 2047, 2212, 2367, 2469, 2576, 2803
Mva1269I GAATGC 1 cut(s) 904
MvaI CCWGG 6 cut(s) 1205, 2047, 2367, 2469, 2576, 2803
MvnI CGCG 2 cut(s) 92, 94
NciI CCSGG 2 cut(s) 631, 2212
NcoI CCATGG 1 cut(s) 1929
NdeI CATATG 1 cut(s) 1253
NlaIV GGNNCC 7 cut(s) 441, 717, 1601, 1725, 1815, 2209, 2838
NmeAIII GCCGAG 1 cut(s) 3029
NmuCI GTSAC 5 cut(s) 286, 1703, 2479, 3302, 3311
NsiI ATGCAT 3 cut(s) 211, 667, 703
NspI RCATGY 4 cut(s) 209, 665, 839, 1540
NspV TTCGAA 1 cut(s) 1638
OliI CACNNNNGTG 1 cut(s) 3309
PaeI GCATGC 2 cut(s) 209, 1540
PaeR7I CTCGAG 1 cut(s) 2916
PagI TCATGA 1 cut(s) 3617
PauI GCGCGC 1 cut(s) 92
PciI ACATGT 1 cut(s) 835
PcsI WCGNNNNNNNCGW 1 cut(s) 1960
PctI GAATGC 1 cut(s) 904
PdmI GAANNNNTTC 4 cut(s) 408, 1305, 1739, 1992
PfeI GAWTC 7 cut(s) 119, 863, 1306, 2516, 2701, 3035, 3269
PfoI TCCNGGA 1 cut(s) 2467
PleI GAGTC 2 cut(s) 844, 3014
PpsI GAGTC 2 cut(s) 844, 3014
PpuMI RGGWCCY 1 cut(s) 440
PscI ACATGT 1 cut(s) 835
PshAI GACNNNNGTC 1 cut(s) 786
PsiI TTATAA 1 cut(s) 3527
Psp124BI GAGCTC 3 cut(s) 80, 1785, 3053
Psp1406I AACGTT 1 cut(s) 3564
Psp5II RGGWCCY 1 cut(s) 440
Psp6I CCWGG 6 cut(s) 1203, 2045, 2365, 2467, 2574, 2801
PspFI CCCAGC 2 cut(s) 938, 2861
PspGI CCWGG 6 cut(s) 1203, 2045, 2365, 2467, 2574, 2801
PspN4I GGNNCC 7 cut(s) 441, 717, 1601, 1725, 1815, 2209, 2838
PspPI GGNCC 8 cut(s) 232, 440, 646, 1600, 1724, 2465, 2572, 2805
PspPPI RGGWCCY 1 cut(s) 440
PstI CTGCAG 2 cut(s) 673, 3105
PstNI CAGNNNCTG 1 cut(s) 406
PsuI RGATCY 5 cut(s) 391, 1813, 2471, 2908, 3232
PteI GCGCGC 1 cut(s) 92
PvuII CAGCTG 1 cut(s) 1190
RsaI GTAC 7 cut(s) 877, 920, 1698, 1858, 2079, 2452, 2561
RsaNI GTAC 7 cut(s) 876, 919, 1697, 1857, 2078, 2451, 2560
RseI CAYNNNNRTG 3 cut(s) 756, 798, 3309
SacI GAGCTC 3 cut(s) 80, 1785, 3053
Sau96I GGNCC 8 cut(s) 232, 440, 646, 1600, 1724, 2465, 2572, 2805
ScaI AGTACT 2 cut(s) 877, 1698
SchI GAGTC 2 cut(s) 844, 3015
ScrFI CCNGG 8 cut(s) 631, 1205, 2047, 2212, 2367, 2469, 2576, 2803
SduI GDGCHC 7 cut(s) 80, 718, 1264, 1785, 1834, 2988, 3053
SfcI CTRYAG 3 cut(s) 261, 669, 3101
Sfr274I CTCGAG 1 cut(s) 2916
SfuI TTCGAA 1 cut(s) 1638
SinI GGWCC 6 cut(s) 232, 440, 646, 1724, 2465, 2805
SlaI CTCGAG 1 cut(s) 2916
SmiMI CAYNNNNRTG 3 cut(s) 756, 798, 3309
SmlI CTYRAG 5 cut(s) 1292, 2062, 2195, 2916, 3023
SmoI CTYRAG 5 cut(s) 1292, 2062, 2195, 2916, 3023
SphI GCATGC 2 cut(s) 209, 1540
SsiI CCGC 5 cut(s) 102, 241, 971, 2247, 3345
SspI AATATT 3 cut(s) 961, 977, 1635
SstI GAGCTC 3 cut(s) 80, 1785, 3053
StyD4I CCNGG 8 cut(s) 629, 1203, 2045, 2210, 2365, 2467, 2574, 2801
StyI CCWWGG 5 cut(s) 1929, 2979, 3079, 3253, 3350
TaiI ACGT 1 cut(s) 3567
TaqI TCGA 7 cut(s) 69, 122, 848, 1638, 2917, 3009, 3047
TatI WGTACW 4 cut(s) 875, 918, 1696, 1856
TauI GCSGC 2 cut(s) 104, 2249
TfiI GAWTC 7 cut(s) 119, 863, 1306, 2516, 2701, 3035, 3269
TscAI CASTG 8 cut(s) 295, 2121, 2174, 2560, 2590, 3105, 3292, 3311
TseFI GTSAC 5 cut(s) 286, 1703, 2479, 3302, 3311
Tsp45I GTSAC 5 cut(s) 286, 1703, 2479, 3302, 3311
TspGWI ACGGA 1 cut(s) 776
TspRI CASTG 8 cut(s) 295, 2121, 2174, 2560, 2590, 3105, 3292, 3311
Vha464I CTTAAG 1 cut(s) 2195
VneI GTGCAC 2 cut(s) 1260, 1830
VpaK11BI GGWCC 6 cut(s) 232, 440, 646, 1724, 2465, 2805
XapI RAATTY 7 cut(s) 195, 570, 857, 911, 965, 1851, 2663
XceI RCATGY 4 cut(s) 209, 665, 839, 1540
XhoI CTCGAG 1 cut(s) 2916
XmaJI CCTAGG 1 cut(s) 3350
XmnI GAANNNNTTC 4 cut(s) 408, 1305, 1739, 1992
ZrmI AGTACT 2 cut(s) 877, 1698
Zsp2I ATGCAT 3 cut(s) 211, 667, 703
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.