MD12G1190400.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0005471
Physical Location & Seq
Forward (+)
27318629 .. 27322875
4247 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1190400.v1.1.491

Sequence Viewer

Length: 1443 bp
ATGGTTTCCTCTCAGCTACCTGGTTTGACCAAGGCCCCCATGTGCAAACCAATTCAGACCCAGTTGCTTTTCCCCATGGAAACAGTGGATGCTGCAGCTTCAGATCACCTCGAGCTTGATTTTGATGATGTGTTTGGCCCTCTACCGGTTCCGATCTCAGTAGAAGTGAATTCGGCTAATTTTGCCTCGACTGAAGATGCTACTGAGCTTATTTATGACGATCCAGTGGTTATTCACAACCGATCACATTCGTTGGTTGGTCCCTCATCGTTTGTAAACCAATCATTGAAGCTCAGCAAACTGACTATAAGTGACACAGAAGATTCAGTGGAACTGGTAGAATGTGTCAAAGGAGATACCATCAAAGAATTTCAGGAACCTTTTATTGTTGATGGTGTCAACGAAAAGCCTCTTGAAATTATTGACGAGAACCCCATGAAGATCGAGAGTGTAGGCATTGAAGATTTTGAGGTTTTGAAGGTTGTTGGACAGGGTGCATTTGCAAAAGTATATCAGGTTAGGAAGAAGGGGACATCAGAAATATATGCAATGAAGGTCATGCGGAAGGACAAGATCATGGAGAAAAATCATGCCGAATACATGAAAGCTGAGAGGGATATTTTAACAAAAATCGATCATCCATTCATTATCCAACTTAGATACTCATTTCAGACCAAATATAGACTATATCTTGTGCTGGATTTTGTTAATGGTGGTCACCTTTTCTTTCAGCTCTATCACCATGGCCTTTTTAGAGAGGAGCTGGGACGAATATATGCAGCTGAAATTGTTTCTGCAGTTGCTCACCTCCATGCAAATGGAATAATGCATAGGGATCTTAAACCTGAAAATATCCTATTGGACTCAGATGGCCATGCCATGTTGACTGATTTTGGTCTTGCAAAGCAACTTGAGGAGAATACAAGATCTAATTCCATGTGTGGAACTGTGGAATACATGCCACCCGAAATAGTTCTTGGAAAGGGCCACGATAAGGCTGCAGACTGGTGGAGTGTGGGAGTCCTAATGTTTGAGATGCTTACCGGAAAGCCTCCTTTTACTGGGAACAGGGAGAAAATTCAGCAGAAGATAGTTAAGGAAAAGATCAAGTTGCCATCCTTTTTGTCAAGTGAAGCACATTCTTTGTTGAAAGCGCTGCTACATAAGGATCCAAGCAAGCGCCTTGGCAGTGGGCCTTTGGGGAGTGAGGACATCAAGCGCCACAAATGGTTCAAGCCAATAAATTGGAAAAAACTGGACTCTCGGCAAATCCAACCAAGCTTTCGTCCTGAAGTTGCTGGGAAGCATTGCATTGCCAATTTTGACAAGTGCTGGACTGACATGTCAGTTGTGGATTCCCCAGCTGCTAGCCCGAATGCTGCTGGAAACCCCTTCACCGGCTTCAGTTATGTTAGGCCGGCCTCCTCTTTTCTCCAGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006417 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008285 GO:0009266 GO:0009408 GO:0009409 GO:0009628 GO:0009651 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010608 GO:0010628 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032268 GO:0032270 GO:0034248 GO:0034250 GO:0035556 GO:0036211 GO:0042127 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045727 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051716 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0140096 GO:1901564 GO:2000112
KEGG Pathways
Metabolic & Signaling
ko01521 ko01522 ko04012 ko04066 ko04140 ko04150 ko04151 ko04152 ko04211 ko04212 ko04213 ko04350 ko04371 ko04666 ko04714 ko04910 ko04931 ko05165 ko05200 ko05205 ko05210 ko05212 ko05221 ko05224 ko05225 ko05226 ko05231 map01521 map01522 map04012 map04066 map04140 map04150 map04151 map04152 map04211 map04212 map04213 map04350 map04371 map04666 map04714 map04910 map04931 map05165 map05200 map05205 map05210 map05212 map05221 map05224 map05225 map05226 map05231
Pfam Domains
Protein Families
Pkinase Pkinase_C

Protein Analysis

481

Amino Acids

53.92

Weight (kDa)

6.12

Isoelectric Point (pI)

38.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 156 - 411 4.7e-77 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 157 - 394 5.7e-36 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 230 - 311 1e-06 ABC1 atypical kinase-like domain
Kinase-like PF14531 261 - 390 1.8e-09 Kinase-like
Pkinase_fungal PF17667 263 - 335 9.2e-07 Fungal protein kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1342
AciI CCGC 1 cut(s) 562
AclWI GGATC 4 cut(s) 215, 843, 1163, 1176
AcoI YGGCCR 1 cut(s) 871
AcsI RAATTY 3 cut(s) 169, 368, 1077
AcuI CTGAAG 4 cut(s) 84, 213, 1311, 1387
AfeI AGCGCT 1 cut(s) 1155
AfiI CCNNNNNNNGG 4 cut(s) 145, 994, 1061, 1397
AflIII ACRYGT 1 cut(s) 1341
AgeI ACCGGT 1 cut(s) 145
AgsI TTSAA 6 cut(s) 289, 416, 461, 478, 1150, 1234
AjnI CCWGG 1 cut(s) 19
AjuI GAANNNNNNNTTGG 4 cut(s) 960, 992, 1266, 1298
AlwI GGATC 4 cut(s) 215, 843, 1163, 1176
Ama87I CYCGRG 1 cut(s) 110
Aor51HI AGCGCT 1 cut(s) 1155
AoxI GGCC 8 cut(s) 33, 136, 745, 871, 985, 1193, 1415, 1419
ApeKI GCWGC 7 cut(s) 92, 95, 779, 998, 1156, 1364, 1379
ApoI RAATTY 3 cut(s) 169, 368, 1077
AsiGI ACCGGT 1 cut(s) 145
Asp700I GAANNNNTTC 1 cut(s) 972
AspLEI GCGC 3 cut(s) 1156, 1182, 1221
AspS9I GGNCC 5 cut(s) 34, 137, 260, 985, 1193
AsuHPI GGTGA 5 cut(s) 98, 710, 731, 797, 1387
AsuNHI GCTAGC 1 cut(s) 1367
AvaI CYCGRG 1 cut(s) 110
AvaII GGWCC 1 cut(s) 260
BalI TGGCCA 1 cut(s) 873
BamHI GGATCC 1 cut(s) 1168
BbvI GCAGC 7 cut(s) 79, 107, 791, 985, 1143, 1351, 1366
BccI CCATC 4 cut(s) 368, 386, 863, 1123
BcgI CGANNNNNNTGC 2 cut(s) 980, 1014
BciT130I CCWGG 1 cut(s) 21
BfaI CTAG 1 cut(s) 1368
BfmI CTRYAG 3 cut(s) 93, 795, 999
BfoI RGCGCY 3 cut(s) 1157, 1183, 1222
BglII AGATCT 1 cut(s) 926
BisI GCNGC 7 cut(s) 93, 96, 780, 999, 1157, 1365, 1380
BlpI GCTNAGC 1 cut(s) 293
BlsI GCNGC 7 cut(s) 94, 97, 781, 1000, 1158, 1366, 1381
Bme1390I CCNGG 1 cut(s) 21
Bme18I GGWCC 1 cut(s) 260
BmeT110I CYCGRG 1 cut(s) 110
BmgT120I GGNCC 5 cut(s) 34, 137, 260, 985, 1193
BmiI GGNNCC 5 cut(s) 36, 150, 262, 378, 1170
BmrFI CCNGG 1 cut(s) 21
BmrI ACTGGG 2 cut(s) 55, 1071
BmsI GCATC 3 cut(s) 79, 187, 1026
BmtI GCTAGC 1 cut(s) 1371
BmuI ACTGGG 2 cut(s) 55, 1071
BpmI CTGGAG 1 cut(s) 1418
Bpu1102I GCTNAGC 1 cut(s) 293
BpuEI CTTGAG 1 cut(s) 932
Bsa29I ATCGAT 1 cut(s) 633
BsaJI CCNNGG 4 cut(s) 30, 75, 742, 1183
BsaWI WCCGGW 2 cut(s) 145, 1043
Bsc4I CCNNNNNNNGG 4 cut(s) 145, 994, 1061, 1397
Bse118I RCCGGY 3 cut(s) 145, 1397, 1417
Bse1I ACTGG 6 cut(s) 61, 224, 339, 1010, 1066, 1260
Bse3DI GCAATG 3 cut(s) 555, 1306, 1311
BseBI CCWGG 1 cut(s) 21
BseCI ATCGAT 1 cut(s) 633
BseDI CCNNGG 4 cut(s) 30, 75, 742, 1183
BseGI GGATG 3 cut(s) 94, 637, 1115
BseLI CCNNNNNNNGG 4 cut(s) 145, 994, 1061, 1397
BseMI GCAATG 3 cut(s) 555, 1306, 1311
BseMII CTCAG 6 cut(s) 26, 171, 195, 307, 600, 879
BseNI ACTGG 6 cut(s) 61, 224, 339, 1010, 1066, 1260
BseRI GAGGAG 3 cut(s) 773, 929, 1414
BseXI GCAGC 7 cut(s) 79, 107, 791, 985, 1143, 1351, 1366
BseYI CCCAGC 3 cut(s) 763, 1298, 1360
BshFI GGCC 8 cut(s) 35, 138, 747, 873, 987, 1195, 1417, 1421
BshTI ACCGGT 1 cut(s) 145
BshVI ATCGAT 1 cut(s) 633
BsiHKCI CYCGRG 1 cut(s) 110
BsiSI CCGG 4 cut(s) 146, 1044, 1398, 1418
BslFI GGGAC 3 cut(s) 246, 544, 780
BslI CCNNNNNNNGG 4 cut(s) 145, 994, 1061, 1397
BsmFI GGGAC 3 cut(s) 246, 544, 780
BsmI GAATGC 1 cut(s) 1381
BsnI GGCC 8 cut(s) 35, 138, 747, 873, 987, 1195, 1417, 1421
BsoBI CYCGRG 1 cut(s) 110
Bsp1720I GCTNAGC 1 cut(s) 293
Bsp19I CCATGG 2 cut(s) 75, 742
BspACI CCGC 1 cut(s) 562
BspANI GGCC 8 cut(s) 35, 138, 747, 873, 987, 1195, 1417, 1421
BspCNI CTCAG 6 cut(s) 25, 170, 196, 306, 601, 878
BspDI ATCGAT 1 cut(s) 633
BspLI GGNNCC 5 cut(s) 36, 150, 262, 378, 1170
BspMAI CTGCAG 3 cut(s) 97, 799, 1003
BspOI GCTAGC 1 cut(s) 1371
BspPI GGATC 4 cut(s) 215, 843, 1163, 1176
BsrDI GCAATG 3 cut(s) 555, 1306, 1311
BsrFI RCCGGY 3 cut(s) 145, 1397, 1417
BsrI ACTGG 6 cut(s) 61, 224, 339, 1010, 1066, 1260
BssAI RCCGGY 3 cut(s) 145, 1397, 1417
BssECI CCNNGG 4 cut(s) 30, 75, 742, 1183
BssT1I CCWWGG 4 cut(s) 30, 75, 742, 1183
Bst2UI CCWGG 1 cut(s) 21
Bst4CI ACNGT 2 cut(s) 85, 949
BstC8I GCNNGC 3 cut(s) 1178, 1369, 1419
BstDEI CTNAG 7 cut(s) 12, 157, 204, 293, 609, 656, 865
BstDSI CCRYGG 2 cut(s) 75, 742
BstEII GGTNACC 1 cut(s) 716
BstF5I GGATG 3 cut(s) 94, 637, 1115
BstH2I RGCGCY 3 cut(s) 1157, 1183, 1222
BstHHI GCGC 3 cut(s) 1156, 1182, 1221
BstMWI GCNNNNNNNGC 1 cut(s) 182
BstNI CCWGG 1 cut(s) 21
BstNSI RCATGY 2 cut(s) 961, 1345
BstPI GGTNACC 1 cut(s) 716
BstSCI CCNGG 1 cut(s) 19
BstSFI CTRYAG 3 cut(s) 93, 795, 999
BstV1I GCAGC 7 cut(s) 79, 107, 791, 985, 1143, 1351, 1366
BstX2I RGATCY 3 cut(s) 835, 926, 1168
BstXI CCANNNNNNTGG 2 cut(s) 818, 1245
BstYI RGATCY 3 cut(s) 835, 926, 1168
Bsu15I ATCGAT 1 cut(s) 633
BsuRI GGCC 8 cut(s) 35, 138, 747, 873, 987, 1195, 1417, 1421
BsuTUI ATCGAT 1 cut(s) 633
BtgI CCRYGG 2 cut(s) 75, 742
BtsCI GGATG 3 cut(s) 94, 637, 1115
BtsI GCAGTG 1 cut(s) 1195
BtsIMutI CAGTG 4 cut(s) 90, 231, 333, 1195
Cac8I GCNNGC 3 cut(s) 1178, 1369, 1419
CfoI GCGC 3 cut(s) 1156, 1182, 1221
Cfr10I RCCGGY 3 cut(s) 145, 1397, 1417
Cfr13I GGNCC 5 cut(s) 34, 137, 260, 985, 1193
ClaI ATCGAT 1 cut(s) 633
CsiI ACCWGGT 1 cut(s) 19
CspAI ACCGGT 1 cut(s) 145
DdeI CTNAG 7 cut(s) 12, 157, 204, 293, 609, 656, 865
DrdI GACNNNNNNGTC 1 cut(s) 1342
DseDI GACNNNNNNGTC 1 cut(s) 1342
EaeI YGGCCR 1 cut(s) 871
Eco130I CCWWGG 4 cut(s) 30, 75, 742, 1183
Eco47I GGWCC 1 cut(s) 260
Eco47III AGCGCT 1 cut(s) 1155
Eco57I CTGAAG 4 cut(s) 84, 213, 1311, 1387
Eco88I CYCGRG 1 cut(s) 110
Eco91I GGTNACC 1 cut(s) 716
EcoO109I RGGNCCY 1 cut(s) 34
EcoO65I GGTNACC 1 cut(s) 716
EcoRI GAATTC 1 cut(s) 169
EcoRII CCWGG 1 cut(s) 19
EcoT14I CCWWGG 4 cut(s) 30, 75, 742, 1183
EcoT22I ATGCAT 1 cut(s) 831
ErhI CCWWGG 4 cut(s) 30, 75, 742, 1183
FaqI GGGAC 3 cut(s) 246, 544, 780
Fnu4HI GCNGC 7 cut(s) 93, 96, 780, 999, 1157, 1365, 1380
FokI GGATG 3 cut(s) 101, 624, 1102
FseI GGCCGGCC 1 cut(s) 1421
Fsp4HI GCNGC 7 cut(s) 93, 96, 780, 999, 1157, 1365, 1380
FspBI CTAG 1 cut(s) 1368
GlaI GCGC 3 cut(s) 1155, 1181, 1220
GluI GCNGC 7 cut(s) 93, 96, 780, 999, 1157, 1365, 1380
GsaI CCCAGC 3 cut(s) 767, 1302, 1364
GsuI CTGGAG 1 cut(s) 1418
HaeII RGCGCY 3 cut(s) 1157, 1183, 1222
HaeIII GGCC 8 cut(s) 35, 138, 747, 873, 987, 1195, 1417, 1421
HapII CCGG 4 cut(s) 146, 1044, 1398, 1418
HhaI GCGC 3 cut(s) 1156, 1182, 1221
Hin6I GCGC 3 cut(s) 1154, 1180, 1219
HinP1I GCGC 3 cut(s) 1154, 1180, 1219
HincII GTYRAC 2 cut(s) 400, 885
HindII GTYRAC 2 cut(s) 400, 885
HindIII AAGCTT 1 cut(s) 1279
HinfI GANTC 5 cut(s) 323, 863, 1020, 1259, 1355
HpaII CCGG 4 cut(s) 146, 1044, 1398, 1418
HphI GGTGA 5 cut(s) 98, 710, 731, 797, 1387
Hpy166II GTNNAC 3 cut(s) 277, 400, 885
Hpy188I TCNGA 6 cut(s) 57, 103, 153, 538, 672, 868
Hpy188III TCNNGA 5 cut(s) 374, 413, 445, 1289, 1435
Hpy8I GTNNAC 3 cut(s) 277, 400, 885
HpyAV CCTTC 5 cut(s) 472, 520, 547, 559, 1402
HpyCH4III ACNGT 2 cut(s) 85, 949
HpyF10VI GCNNNNNNNGC 1 cut(s) 182
HpyF3I CTNAG 7 cut(s) 12, 157, 204, 293, 609, 656, 865
HspAI GCGC 3 cut(s) 1154, 1180, 1219
KroI GCCGGC 1 cut(s) 1417
KroNI GCCGGC 1 cut(s) 1419
LmnI GCTCC 1 cut(s) 760
Lsp1109I GCAGC 7 cut(s) 79, 107, 791, 985, 1143, 1351, 1366
LweI GCATC 3 cut(s) 79, 187, 1026
MabI ACCWGGT 1 cut(s) 19
MaeI CTAG 1 cut(s) 1368
MaeIII GTNAC 2 cut(s) 311, 716
MboII GAAGA 6 cut(s) 206, 332, 451, 473, 535, 1099
MflI RGATCY 3 cut(s) 835, 926, 1168
MlsI TGGCCA 1 cut(s) 873
MluNI TGGCCA 1 cut(s) 873
MlyI GAGTC 3 cut(s) 857, 1029, 1253
MmeI TCCRAC 3 cut(s) 466, 676, 1297
Mox20I TGGCCA 1 cut(s) 873
Mph1103I ATGCAT 1 cut(s) 831
MroNI GCCGGC 1 cut(s) 1417
MroXI GAANNNNTTC 1 cut(s) 972
MscI TGGCCA 1 cut(s) 873
MseI TTAA 4 cut(s) 623, 708, 840, 1095
MslI CAYNNNNRTG 2 cut(s) 810, 816
Msp20I TGGCCA 1 cut(s) 873
MspA1I CMGCKG 2 cut(s) 782, 1364
MspI CCGG 4 cut(s) 146, 1044, 1398, 1418
MspR9I CCNGG 1 cut(s) 21
Mva1269I GAATGC 1 cut(s) 1381
MvaI CCWGG 1 cut(s) 21
MwoI GCNNNNNNNGC 1 cut(s) 182
NaeI GCCGGC 1 cut(s) 1419
NcoI CCATGG 2 cut(s) 75, 742
NgoMIV GCCGGC 1 cut(s) 1417
NheI GCTAGC 1 cut(s) 1367
NlaIV GGNNCC 5 cut(s) 36, 150, 262, 378, 1170
NmeAIII GCCGAG 1 cut(s) 1243
NmuCI GTSAC 2 cut(s) 311, 716
NsiI ATGCAT 1 cut(s) 831
NspI RCATGY 2 cut(s) 961, 1345
PaeR7I CTCGAG 1 cut(s) 110
PciI ACATGT 1 cut(s) 1341
PctI GAATGC 1 cut(s) 1381
PdiI GCCGGC 1 cut(s) 1419
PdmI GAANNNNTTC 1 cut(s) 972
PfeI GAWTC 2 cut(s) 323, 1355
PinAI ACCGGT 1 cut(s) 145
PkrI GCNGC 7 cut(s) 94, 97, 781, 1000, 1158, 1366, 1381
PleI GAGTC 3 cut(s) 857, 1028, 1253
PpsI GAGTC 3 cut(s) 857, 1028, 1253
PscI ACATGT 1 cut(s) 1341
Psp6I CCWGG 1 cut(s) 19
PspEI GGTNACC 1 cut(s) 716
PspFI CCCAGC 3 cut(s) 763, 1298, 1360
PspGI CCWGG 1 cut(s) 19
PspN4I GGNNCC 5 cut(s) 36, 150, 262, 378, 1170
PspPI GGNCC 5 cut(s) 34, 137, 260, 985, 1193
PspXI VCTCGAGB 1 cut(s) 110
PstI CTGCAG 3 cut(s) 97, 799, 1003
PsuI RGATCY 3 cut(s) 835, 926, 1168
PvuII CAGCTG 2 cut(s) 782, 1364
RigI GGCCGGCC 1 cut(s) 1421
RseI CAYNNNNRTG 2 cut(s) 810, 816
SaqAI TTAA 4 cut(s) 623, 708, 840, 1095
SatI GCNGC 7 cut(s) 93, 96, 780, 999, 1157, 1365, 1380
Sau96I GGNCC 5 cut(s) 34, 137, 260, 985, 1193
SchI GAGTC 3 cut(s) 857, 1029, 1253
ScrFI CCNGG 1 cut(s) 21
SexAI ACCWGGT 1 cut(s) 19
SfaNI GCATC 3 cut(s) 79, 187, 1026
SfcI CTRYAG 3 cut(s) 93, 795, 999
Sfr274I CTCGAG 1 cut(s) 110
SinI GGWCC 1 cut(s) 260
SlaI CTCGAG 1 cut(s) 110
SmiMI CAYNNNNRTG 2 cut(s) 810, 816
SmlI CTYRAG 2 cut(s) 110, 911
SmoI CTYRAG 2 cut(s) 110, 911
SsiI CCGC 1 cut(s) 562
SspMI CTAG 1 cut(s) 1368
StyD4I CCNGG 1 cut(s) 19
StyI CCWWGG 4 cut(s) 30, 75, 742, 1183
TaaI ACNGT 2 cut(s) 85, 949
TaqI TCGA 4 cut(s) 111, 188, 444, 633
TfiI GAWTC 2 cut(s) 323, 1355
Tru1I TTAA 4 cut(s) 623, 708, 840, 1095
Tru9I TTAA 4 cut(s) 623, 708, 840, 1095
TscAI CASTG 4 cut(s) 90, 231, 333, 1195
TseFI GTSAC 2 cut(s) 311, 716
TseI GCWGC 7 cut(s) 92, 95, 779, 998, 1156, 1364, 1379
Tsp45I GTSAC 2 cut(s) 311, 716
TspDTI ATGAA 4 cut(s) 452, 566, 617, 634
TspRI CASTG 4 cut(s) 90, 231, 333, 1195
VpaK11BI GGWCC 1 cut(s) 260
XapI RAATTY 3 cut(s) 169, 368, 1077
XceI RCATGY 2 cut(s) 961, 1345
XcmI CCANNNNNNNNNTGG 1 cut(s) 82
XhoI CTCGAG 1 cut(s) 110
XmnI GAANNNNTTC 1 cut(s) 972
XspI CTAG 1 cut(s) 1368
Zsp2I ATGCAT 1 cut(s) 831
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.