RchiOBHm_Chr3g0460281

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0005471
Physical Location & Seq
Reverse (-)
8440621 .. 8444396
3776 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42681

Sequence Viewer

Length: 1437 bp
ATGGTTTTATCGAAGCTACCTGATTCGTCCAAGACCCGCAAGTGCAATCCAGTTCAGACCCAGTTGCTTTTTCCAATGGGCCAGATGGATGCTGTTGTGTCCGATCACGTTGAGCTTGACTTTTCAGATGTATTTGGTCCTGTACCAGTCAATGCCCCTGTAGACTCGGCTAATTCTGCCTCTGTTGAAGATGGCGCTGACCATATATATGATGATCCTGTCGTCATTCACAACCGATCACATTCTTTGGTCGGTCCTTCATCGTATGTAAGCCATTCATTGAAGCTCAGCAAACTCACTATTCATGACACAGAAGATTCAGTGGATCTGGTAGAGTGTCTCCATGAAGATACCGTGAAAGAATCTCAGGAACCTTTTATTGGTGATGTTGTCCTTGGAAAACCTCTACAAGTTGTTGAGGAGAATTCCATGAAGACTGAGAGTGTAGGCATTGAAGATTTTGAGGTTTTGAAGGTTGTTGGGCAGGGTGCATTTGCAAAAGTATATCAGGTGAGGAAGAAGGGGACATCAGAAATATATGCTATGAAGGTCATGCGAAAGGATAAGATTATGGAGAAGAACCATGCTGAATACATGAAAGCTGAGAGGGATATATTAACAAAAGTTGATCATCCGTTCATTATCCAGCTTAGATACTCATTCCAAACCAAATACAGACTTTATCTCGTGCTGGATTTTGTCAATGGAGGTCACCTTTTCTTTCAGCTCTACCACCATGGCCTTTTCAGGGAAGAGCTGGCACGAATATATGCTGCGGAGATTGTTTCTGCTGTATCTCACCTCCATGCAAATGGCATAATGCATAGGGATCTTAAACCTGAAAATATCTTATTGGATGCAGATGGCCATGCCATGTTGACTGATTTTGGGCTTGCAAAGAAATTTGACGAAAATACAAGATCTAATTCTCTATGTGGAACTGTAGAATACATGGCACCTGAAATAATTCAGGGAAAGGGCCATGATAAGGCAGCAGATTGGTGGAGTGTGGGAGTTCTATTGTTTGAGATGCTTACTGGAAAGCCTCCTTTTACGGGGAACCGTCAGAAAATTCAACAGAAGATAGTTAAGGACAAAGTCAAGTTGCCAACCTTTTTAACAAGTGAAGCACATTCTCTGGTGAAAGCGCTATTAGAGAAGGACACCAGCAAGCGCCTGGGTTGTGGACCTCTGGGAAGTGATGAAATCAAGCGCCACAAATGGTTCAAGCCAATTAATTGGAAGAAATTGGATGCTCGCGAAATCCAACCAAGCTTCCGACCTGATGTTGCTGGGCAGCACTGCATCGCCAACTTTGAGAAATGCTGGACTGACATGCCACTTGTGGACTCCCCAGCTGCTAGTCCGAATGCTTCTGGAAACCCGTTCTCAGGCTTTAGTTATGTTAGGCCTGCGGTCTCTTTTCTTCAGAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006417 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008285 GO:0009266 GO:0009408 GO:0009409 GO:0009628 GO:0009651 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010608 GO:0010628 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032268 GO:0032270 GO:0034248 GO:0034250 GO:0035556 GO:0036211 GO:0042127 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045727 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051716 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0140096 GO:1901564 GO:2000112
KEGG Pathways
Metabolic & Signaling
ko01521 ko01522 ko04012 ko04066 ko04140 ko04150 ko04151 ko04152 ko04211 ko04212 ko04213 ko04350 ko04371 ko04666 ko04714 ko04910 ko04931 ko05165 ko05200 ko05205 ko05210 ko05212 ko05221 ko05224 ko05225 ko05226 ko05231 map01521 map01522 map04012 map04066 map04140 map04150 map04151 map04152 map04211 map04212 map04213 map04350 map04371 map04666 map04714 map04910 map04931 map05165 map05200 map05205 map05210 map05212 map05221 map05224 map05225 map05226 map05231
Pfam Domains
Protein Families
Pkinase Pkinase_C

Protein Analysis

478

Amino Acids

53.61

Weight (kDa)

6.29

Isoelectric Point (pI)

36.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 154 - 409 9.9e-77 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 155 - 392 4.1e-36 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 228 - 309 9.3e-07 ABC1 atypical kinase-like domain
Kinase-like PF14531 252 - 392 1.1e-07 Kinase-like
Pkinase_fungal PF17667 261 - 333 2.9e-08 Fungal protein kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 955
AccI GTMKAC 1 cut(s) 162
AccII CGCG 1 cut(s) 1260
AciI CCGC 3 cut(s) 37, 776, 1415
AclWI GGATC 3 cut(s) 209, 333, 837
AcoI YGGCCR 1 cut(s) 865
AcsI RAATTY 3 cut(s) 424, 902, 1071
AcuI CTGAAG 1 cut(s) 1412
AfaI GTAC 1 cut(s) 144
AfeI AGCGCT 1 cut(s) 1149
AfiI CCNNNNNNNGG 5 cut(s) 380, 748, 988, 1055, 1391
AgsI TTSAA 6 cut(s) 188, 283, 455, 472, 1076, 1228
AjnI CCWGG 1 cut(s) 1176
AjuI GAANNNNNNNTTGG 2 cut(s) 363, 395
AluBI AGCT 9 cut(s) 16, 115, 286, 602, 649, 727, 757, 1275, 1358
AluI AGCT 9 cut(s) 16, 115, 286, 602, 649, 727, 757, 1275, 1358
Alw26I GTCTC 2 cut(s) 344, 1423
AlwI GGATC 3 cut(s) 209, 333, 837
Aor51HI AGCGCT 1 cut(s) 1149
AoxI GGCC 5 cut(s) 79, 739, 865, 979, 1409
ApeKI GCWGC 4 cut(s) 773, 992, 1297, 1358
ApoI RAATTY 3 cut(s) 424, 902, 1071
AseI ATTAAT 1 cut(s) 1236
Asp700I GAANNNNTTC 1 cut(s) 966
AspLEI GCGC 4 cut(s) 197, 1150, 1176, 1215
AspS9I GGNCC 5 cut(s) 79, 137, 254, 979, 1187
AsuHPI GGTGA 5 cut(s) 395, 523, 704, 791, 1153
AvaII GGWCC 3 cut(s) 137, 254, 1187
BalI TGGCCA 1 cut(s) 867
BanI GGYRCC 1 cut(s) 955
BauI CACGAG 1 cut(s) 686
BbsI GAAGAC 1 cut(s) 440
BbvI GCAGC 4 cut(s) 760, 1004, 1309, 1345
BccI CCATC 3 cut(s) 79, 185, 857
BciT130I CCWGG 1 cut(s) 1178
BclI TGATCA 1 cut(s) 628
BcoDI GTCTC 2 cut(s) 344, 1423
BfaI CTAG 2 cut(s) 1362, 1435
BfmI CTRYAG 2 cut(s) 159, 942
BfoI RGCGCY 4 cut(s) 198, 1151, 1177, 1216
BglII AGATCT 1 cut(s) 920
BisI GCNGC 4 cut(s) 774, 993, 1298, 1359
BlpI GCTNAGC 1 cut(s) 287
BlsI GCNGC 4 cut(s) 775, 994, 1299, 1360
Bme1390I CCNGG 1 cut(s) 1178
Bme18I GGWCC 3 cut(s) 137, 254, 1187
BmgT120I GGNCC 5 cut(s) 79, 137, 254, 979, 1187
BmiI GGNNCC 3 cut(s) 372, 957, 1061
BmrFI CCNGG 1 cut(s) 1178
BmrI ACTGGG 1 cut(s) 55
BmsI GCATC 5 cut(s) 79, 847, 1020, 1243, 1314
BmuI ACTGGG 1 cut(s) 55
BpiI GAAGAC 1 cut(s) 440
Bpu1102I GCTNAGC 1 cut(s) 287
BsaI GGTCTC 1 cut(s) 1423
BsaJI CCNNGG 3 cut(s) 394, 736, 1177
Bsc4I CCNNNNNNNGG 5 cut(s) 380, 748, 988, 1055, 1391
Bse1I ACTGG 4 cut(s) 50, 61, 146, 1042
BseBI CCWGG 1 cut(s) 1178
BseDI CCNNGG 3 cut(s) 394, 736, 1177
BseGI GGATG 4 cut(s) 94, 631, 862, 1258
BseLI CCNNNNNNNGG 5 cut(s) 380, 748, 988, 1055, 1391
BseMII CTCAG 5 cut(s) 301, 380, 429, 594, 1404
BseNI ACTGG 4 cut(s) 50, 61, 146, 1042
BseRI GAGGAG 1 cut(s) 434
BseXI GCAGC 4 cut(s) 760, 1004, 1309, 1345
BseYI CCCAGC 2 cut(s) 1292, 1354
Bsh1236I CGCG 1 cut(s) 1260
BshFI GGCC 5 cut(s) 81, 741, 867, 981, 1411
BshNI GGYRCC 1 cut(s) 955
BslFI GGGAC 1 cut(s) 538
BslI CCNNNNNNNGG 5 cut(s) 380, 748, 988, 1055, 1391
BsmAI GTCTC 2 cut(s) 344, 1423
BsmFI GGGAC 1 cut(s) 538
BsmI GAATGC 1 cut(s) 1375
BsnI GGCC 5 cut(s) 81, 741, 867, 981, 1411
Bso31I GGTCTC 1 cut(s) 1423
Bsp143I GATC 7 cut(s) 103, 214, 236, 325, 628, 829, 920
Bsp1720I GCTNAGC 1 cut(s) 287
Bsp19I CCATGG 1 cut(s) 736
Bsp68I TCGCGA 1 cut(s) 1260
BspACI CCGC 3 cut(s) 37, 776, 1415
BspANI GGCC 5 cut(s) 81, 741, 867, 981, 1411
BspCNI CTCAG 5 cut(s) 300, 379, 430, 595, 1403
BspFNI CGCG 1 cut(s) 1260
BspHI TCATGA 1 cut(s) 304
BspLI GGNNCC 3 cut(s) 372, 957, 1061
BspPI GGATC 3 cut(s) 209, 333, 837
BspQI GCTCTTC 1 cut(s) 747
BspT107I GGYRCC 1 cut(s) 955
BspTNI GGTCTC 1 cut(s) 1423
BsrI ACTGG 4 cut(s) 50, 61, 146, 1042
BssECI CCNNGG 3 cut(s) 394, 736, 1177
BssMI GATC 7 cut(s) 103, 214, 236, 325, 628, 829, 920
BssSI CACGAG 1 cut(s) 686
BssT1I CCWWGG 2 cut(s) 394, 736
Bst2BI CACGAG 1 cut(s) 686
Bst2UI CCWGG 1 cut(s) 1178
Bst4CI ACNGT 3 cut(s) 355, 943, 1064
Bst6I CTCTTC 1 cut(s) 747
BstC8I GCNNGC 5 cut(s) 759, 894, 1172, 1258, 1413
BstDEI CTNAG 6 cut(s) 287, 366, 438, 603, 650, 1390
BstDSI CCRYGG 1 cut(s) 736
BstEII GGTNACC 1 cut(s) 710
BstF5I GGATG 4 cut(s) 94, 631, 862, 1258
BstFNI CGCG 1 cut(s) 1260
BstH2I RGCGCY 4 cut(s) 198, 1151, 1177, 1216
BstHHI GCGC 4 cut(s) 197, 1150, 1176, 1215
BstKTI GATC 7 cut(s) 106, 217, 239, 328, 631, 832, 923
BstMAI GTCTC 2 cut(s) 344, 1423
BstMBI GATC 7 cut(s) 103, 214, 236, 325, 628, 829, 920
BstMWI GCNNNNNNNGC 1 cut(s) 176
BstNI CCWGG 1 cut(s) 1178
BstNSI RCATGY 1 cut(s) 1339
BstPI GGTNACC 1 cut(s) 710
BstSCI CCNGG 1 cut(s) 1176
BstSFI CTRYAG 2 cut(s) 159, 942
BstUI CGCG 1 cut(s) 1260
BstV1I GCAGC 4 cut(s) 760, 1004, 1309, 1345
BstV2I GAAGAC 1 cut(s) 440
BstX2I RGATCY 3 cut(s) 325, 829, 920
BstXI CCANNNNNNTGG 2 cut(s) 812, 1239
BstYI RGATCY 3 cut(s) 325, 829, 920
BsuRI GGCC 5 cut(s) 81, 741, 867, 981, 1411
BtgI CCRYGG 1 cut(s) 736
BtgZI GCGATG 1 cut(s) 1291
BtsCI GGATG 4 cut(s) 94, 631, 862, 1258
BtsI GCAGTG 1 cut(s) 1300
BtsIMutI CAGTG 2 cut(s) 327, 1300
BtuMI TCGCGA 1 cut(s) 1260
Cac8I GCNNGC 5 cut(s) 759, 894, 1172, 1258, 1413
CciI TCATGA 1 cut(s) 304
CfoI GCGC 4 cut(s) 197, 1150, 1176, 1215
Cfr13I GGNCC 5 cut(s) 79, 137, 254, 979, 1187
Csp6I GTAC 1 cut(s) 143
CviQI GTAC 1 cut(s) 143
DdeI CTNAG 6 cut(s) 287, 366, 438, 603, 650, 1390
DpnI GATC 7 cut(s) 105, 216, 238, 327, 630, 831, 922
DpnII GATC 7 cut(s) 103, 214, 236, 325, 628, 829, 920
EaeI YGGCCR 1 cut(s) 865
Eam1104I CTCTTC 1 cut(s) 747
EarI CTCTTC 1 cut(s) 747
Eco130I CCWWGG 2 cut(s) 394, 736
Eco147I AGGCCT 1 cut(s) 1411
Eco31I GGTCTC 1 cut(s) 1423
Eco47I GGWCC 3 cut(s) 137, 254, 1187
Eco47III AGCGCT 1 cut(s) 1149
Eco57I CTGAAG 1 cut(s) 1412
Eco91I GGTNACC 1 cut(s) 710
EcoO65I GGTNACC 1 cut(s) 710
EcoRI GAATTC 1 cut(s) 424
EcoRII CCWGG 1 cut(s) 1176
EcoT14I CCWWGG 2 cut(s) 394, 736
EcoT22I ATGCAT 1 cut(s) 825
ErhI CCWWGG 2 cut(s) 394, 736
FaqI GGGAC 1 cut(s) 538
FauI CCCGC 1 cut(s) 44
FbaI TGATCA 1 cut(s) 628
FblI GTMKAC 1 cut(s) 162
Fnu4HI GCNGC 4 cut(s) 774, 993, 1298, 1359
FokI GGATG 4 cut(s) 101, 618, 869, 1265
Fsp4HI GCNGC 4 cut(s) 774, 993, 1298, 1359
FspBI CTAG 2 cut(s) 1362, 1435
GlaI GCGC 4 cut(s) 196, 1149, 1175, 1214
GluI GCNGC 4 cut(s) 774, 993, 1298, 1359
GsaI CCCAGC 2 cut(s) 1296, 1358
HaeII RGCGCY 4 cut(s) 198, 1151, 1177, 1216
HaeIII GGCC 5 cut(s) 81, 741, 867, 981, 1411
HhaI GCGC 4 cut(s) 197, 1150, 1176, 1215
Hin6I GCGC 4 cut(s) 195, 1148, 1174, 1213
HinP1I GCGC 4 cut(s) 195, 1148, 1174, 1213
HincII GTYRAC 1 cut(s) 879
HindII GTYRAC 1 cut(s) 879
HindIII AAGCTT 1 cut(s) 1273
HinfI GANTC 5 cut(s) 23, 164, 317, 362, 1349
HphI GGTGA 5 cut(s) 395, 523, 704, 791, 1153
Hpy166II GTNNAC 4 cut(s) 163, 879, 1187, 1348
Hpy188I TCNGA 8 cut(s) 57, 103, 127, 532, 1068, 1280, 1368, 1431
Hpy188III TCNNGA 4 cut(s) 305, 368, 1259, 1377
Hpy8I GTNNAC 4 cut(s) 163, 879, 1187, 1348
HpyAV CCTTC 5 cut(s) 267, 466, 514, 541, 1153
HpyCH4III ACNGT 3 cut(s) 355, 943, 1064
HpyCH4IV ACGT 1 cut(s) 108
HpyCH4V TGCA 8 cut(s) 45, 491, 497, 809, 823, 860, 896, 1305
HpyF10VI GCNNNNNNNGC 1 cut(s) 176
HpyF3I CTNAG 6 cut(s) 287, 366, 438, 603, 650, 1390
HpySE526I ACGT 1 cut(s) 108
HspAI GCGC 4 cut(s) 195, 1148, 1174, 1213
Ksp22I TGATCA 1 cut(s) 628
Kzo9I GATC 7 cut(s) 103, 214, 236, 325, 628, 829, 920
LguI GCTCTTC 1 cut(s) 747
Lsp1109I GCAGC 4 cut(s) 760, 1004, 1309, 1345
LweI GCATC 5 cut(s) 79, 847, 1020, 1243, 1314
MaeI CTAG 2 cut(s) 1362, 1435
MaeII ACGT 1 cut(s) 108
MaeIII GTNAC 1 cut(s) 710
MalI GATC 7 cut(s) 105, 216, 238, 327, 630, 831, 922
MboI GATC 7 cut(s) 103, 214, 236, 325, 628, 829, 920
MflI RGATCY 3 cut(s) 325, 829, 920
MlsI TGGCCA 1 cut(s) 867
MluCI AATT 9 cut(s) 172, 424, 902, 925, 966, 1071, 1233, 1237, 1247
MluNI TGGCCA 1 cut(s) 867
MlyI GAGTC 2 cut(s) 158, 1343
MmeI TCCRAC 2 cut(s) 1291, 1303
Mox20I TGGCCA 1 cut(s) 867
Mph1103I ATGCAT 1 cut(s) 825
MroXI GAANNNNTTC 1 cut(s) 966
MscI TGGCCA 1 cut(s) 867
MseI TTAA 5 cut(s) 617, 834, 1089, 1118, 1236
MslI CAYNNNNRTG 3 cut(s) 207, 804, 810
Msp20I TGGCCA 1 cut(s) 867
MspA1I CMGCKG 1 cut(s) 1358
MspR9I CCNGG 1 cut(s) 1178
Mva1269I GAATGC 1 cut(s) 1375
MvaI CCWGG 1 cut(s) 1178
MvnI CGCG 1 cut(s) 1260
MwoI GCNNNNNNNGC 1 cut(s) 176
NcoI CCATGG 1 cut(s) 736
NdeII GATC 7 cut(s) 103, 214, 236, 325, 628, 829, 920
NlaIV GGNNCC 3 cut(s) 372, 957, 1061
NmeAIII GCCGAG 1 cut(s) 146
NmuCI GTSAC 1 cut(s) 710
NruI TCGCGA 1 cut(s) 1260
NsiI ATGCAT 1 cut(s) 825
NspI RCATGY 1 cut(s) 1339
PagI TCATGA 1 cut(s) 304
PceI AGGCCT 1 cut(s) 1411
PciSI GCTCTTC 1 cut(s) 747
PctI GAATGC 1 cut(s) 1375
PdmI GAANNNNTTC 1 cut(s) 966
PfeI GAWTC 3 cut(s) 23, 317, 362
PflFI GACNNNGTC 1 cut(s) 1097
PkrI GCNGC 4 cut(s) 775, 994, 1299, 1360
PleI GAGTC 2 cut(s) 158, 1343
PpsI GAGTC 2 cut(s) 158, 1343
PshBI ATTAAT 1 cut(s) 1236
Psp6I CCWGG 1 cut(s) 1176
PspEI GGTNACC 1 cut(s) 710
PspFI CCCAGC 2 cut(s) 1292, 1354
PspGI CCWGG 1 cut(s) 1176
PspN4I GGNNCC 3 cut(s) 372, 957, 1061
PspPI GGNCC 5 cut(s) 79, 137, 254, 979, 1187
PsuI RGATCY 3 cut(s) 325, 829, 920
PsyI GACNNNGTC 1 cut(s) 1097
PvuII CAGCTG 1 cut(s) 1358
RruI TCGCGA 1 cut(s) 1260
RsaI GTAC 1 cut(s) 144
RsaNI GTAC 1 cut(s) 143
RseI CAYNNNNRTG 3 cut(s) 207, 804, 810
SapI GCTCTTC 1 cut(s) 747
SaqAI TTAA 5 cut(s) 617, 834, 1089, 1118, 1236
SatI GCNGC 4 cut(s) 774, 993, 1298, 1359
Sau3AI GATC 7 cut(s) 103, 214, 236, 325, 628, 829, 920
Sau96I GGNCC 5 cut(s) 79, 137, 254, 979, 1187
SchI GAGTC 2 cut(s) 158, 1343
ScrFI CCNGG 1 cut(s) 1178
SfaNI GCATC 5 cut(s) 79, 847, 1020, 1243, 1314
SfcI CTRYAG 2 cut(s) 159, 942
SinI GGWCC 3 cut(s) 137, 254, 1187
SmiMI CAYNNNNRTG 3 cut(s) 207, 804, 810
Sse9I AATT 9 cut(s) 172, 424, 902, 925, 966, 1071, 1233, 1237, 1247
SseBI AGGCCT 1 cut(s) 1411
SsiI CCGC 3 cut(s) 37, 776, 1415
SspMI CTAG 2 cut(s) 1362, 1435
StuI AGGCCT 1 cut(s) 1411
StyD4I CCNGG 1 cut(s) 1176
StyI CCWWGG 2 cut(s) 394, 736
TaaI ACNGT 3 cut(s) 355, 943, 1064
TaiI ACGT 1 cut(s) 111
TaqI TCGA 1 cut(s) 11
TaqII GACCGA 1 cut(s) 242
TasI AATT 9 cut(s) 172, 424, 902, 925, 966, 1071, 1233, 1237, 1247
TfiI GAWTC 3 cut(s) 23, 317, 362
Tru1I TTAA 5 cut(s) 617, 834, 1089, 1118, 1236
Tru9I TTAA 5 cut(s) 617, 834, 1089, 1118, 1236
TscAI CASTG 2 cut(s) 327, 1307
TseFI GTSAC 1 cut(s) 710
TseI GCWGC 4 cut(s) 773, 992, 1297, 1358
Tsp45I GTSAC 1 cut(s) 710
TspDTI ATGAA 9 cut(s) 249, 267, 293, 360, 446, 560, 611, 628, 1218
TspGWI ACGGA 1 cut(s) 624
TspRI CASTG 2 cut(s) 327, 1307
Tth111I GACNNNGTC 1 cut(s) 1097
VpaK11BI GGWCC 3 cut(s) 137, 254, 1187
VspI ATTAAT 1 cut(s) 1236
XapI RAATTY 3 cut(s) 424, 902, 1071
XceI RCATGY 1 cut(s) 1339
XcmI CCANNNNNNNNNTGG 1 cut(s) 1174
XmiI GTMKAC 1 cut(s) 162
XmnI GAANNNNTTC 1 cut(s) 966
XspI CTAG 2 cut(s) 1362, 1435
Zsp2I ATGCAT 1 cut(s) 825
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.