Rh6DG323500

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0005471
Physical Location & Seq
Reverse (-)
52847332 .. 52855023
7692 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG323500.1

Sequence Viewer

Length: 1392 bp
ATGGTTTTCAGACCCGAGTGCAAACCAATTCAGACTCGGTTGCTTTTTCCAATTTGCATGATGGATGCTGTAGTTCCACATCAAGTTGAGCATGACTTTTCAGATGTATTTGGTCCTGTACCAGTCCAGACCCCAGTGGATTCAGCTAATTCTGCCTCTCTCCAAGATAGTACGGATCCCGTCGTCATTCACAACCTGTCACACTCTTTGATTGGTCCTTCATTCTATGTAAGCCATTCACTGAACCTCAACAAACTGACTATTTATAACACAGAAGATTCAGTGGACCTAGCAGAGTACTGTCTCCATGAAGAAACCATCAAAGAATCTCAGGAACCTTTTGTCGGTGACCGTGTCATTGGGCATCCTCTAGAAAATGTTGAGGAGAGTTTCTTGAAGAGTGAGAGTGTAGGCATCGAAGATTTTGAGGTTTTGAAGATTGTTGGGCAGGGTGGATTTGCAAAAGTATATCAGGTGAGGAAGAAGGGAACCTCAGAAATATACGCGATGAAGGTAATGCGAAAGGACAAGGTCATGAAGAATAATCTCGCTGAATACATGAAATTTGAGAGGGATATATTAACAAAAGTTGATCATCCATTCATTGTCAAGCTTAGATACTCATTCCAAACCAAATACAGACTATATCTTGTGCTGGATTTTGTCAACGGAGGTCAGCTTTTCTTTCAACTCTACCACCATGGCCTTTTTTTGGAGGAGCTGGCACGAATATATGCAGCCGAGATTGTTTCTGCGGTGTCTCACCTTCATTCAAATGGCATAATGCATAGAGATCTTAAGCCTGAAAATATCCTATTGGATGCAGATGGTCATGCCATTTTAACTGATTTTGGGCTTGCGAAGAGCTTTGACGAAAACACGAGGTCTAATTCTCTATGTGGAACTGTAGAATACATGGCACCGGAAATAGTTCAGGGAAATGGCCATGATAAGGCAGCAGATTGGTGGAGTGTGGGAGTTGTATTGTTTCAGATGCTTACCGGCAAGCTTCCTTTTACTGGGAACCGTCAGAAATGTCAAGAGAAGATAGTAAAGGAGAAAATCAAGTTGCCAAGTTTTTTGTCAAGTGAAGCACACTCTCTGTTGAAAGGCTTGTTAGCGAAGGACACAAGCAAGCGTCTAGGTTGTGGACCTCTGGGAGGTGATGAAGTCAAGCAGCACAAATGGTTCAAGCAAATTAATTGGAAGAAATTGGATGCTCGTGAGATGAAACCAAGCTTCCAACCCAACGTTGCCGGGCAGCACTGCATCGCCAACATTGAGAATTACAGGACTGACATGCTACTTGTGGACTCCCCGGCTGCTAGTCCGAAGGCTTCGTCTGGAAACCCCTTCTCCGGCTTTAGTTATGTTAGGCCTGCAGCCTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006417 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008285 GO:0009266 GO:0009408 GO:0009409 GO:0009628 GO:0009651 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010608 GO:0010628 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032268 GO:0032270 GO:0034248 GO:0034250 GO:0035556 GO:0036211 GO:0042127 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045727 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051716 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0140096 GO:1901564 GO:2000112
KEGG Pathways
Metabolic & Signaling
ko01521 ko01522 ko04012 ko04066 ko04140 ko04150 ko04151 ko04152 ko04211 ko04212 ko04213 ko04350 ko04371 ko04666 ko04714 ko04910 ko04931 ko05165 ko05200 ko05205 ko05210 ko05212 ko05221 ko05224 ko05225 ko05226 ko05231 map01521 map01522 map04012 map04066 map04140 map04150 map04151 map04152 map04211 map04212 map04213 map04350 map04371 map04666 map04714 map04910 map04931 map05165 map05200 map05205 map05210 map05212 map05221 map05224 map05225 map05226 map05231
Pfam Domains
Protein Families
Pkinase Pkinase_C

Protein Analysis

463

Amino Acids

52.04

Weight (kDa)

6.42

Isoelectric Point (pI)

38.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 142 - 397 1.8e-74 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 144 - 380 1.6e-33 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 216 - 297 2.3e-06 ABC1 atypical kinase-like domain
Kinase-like PF14531 238 - 380 2.3e-09 Kinase-like
Pkinase_fungal PF17667 249 - 329 7.2e-08 Fungal protein kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 267
AccB1I GGYRCC 1 cut(s) 919
AccII CGCG 1 cut(s) 506
AciI CCGC 1 cut(s) 755
AclI AACGTT 1 cut(s) 1251
AclWI GGATC 2 cut(s) 170, 183
AcoI YGGCCR 1 cut(s) 943
AcsI RAATTY 1 cut(s) 563
AfaI GTAC 3 cut(s) 120, 172, 299
AfiI CCNNNNNNNGG 6 cut(s) 344, 712, 952, 1019, 1160, 1358
AflII CTTAAG 1 cut(s) 797
AgsI TTSAA 6 cut(s) 397, 436, 689, 774, 1108, 1192
AluBI AGCT 7 cut(s) 146, 613, 679, 721, 867, 1009, 1239
AluI AGCT 7 cut(s) 146, 613, 679, 721, 867, 1009, 1239
Alw26I GTCTC 2 cut(s) 308, 765
AlwI GGATC 2 cut(s) 170, 183
Ama87I CYCGRG 1 cut(s) 14
AoxI GGCC 3 cut(s) 703, 943, 1376
ApeKI GCWGC 6 cut(s) 737, 956, 1177, 1261, 1322, 1382
ApoI RAATTY 1 cut(s) 563
ArsI GACNNNNNNTTYG 2 cut(s) 1325, 1357
AseI ATTAAT 1 cut(s) 1200
Asp700I GAANNNNTTC 1 cut(s) 930
AspS9I GGNCC 4 cut(s) 113, 215, 286, 1151
AsuC2I CCSGG 2 cut(s) 1258, 1319
AsuHPI GGTGA 4 cut(s) 359, 487, 755, 1175
AvaI CYCGRG 1 cut(s) 14
AvaII GGWCC 4 cut(s) 113, 215, 286, 1151
BalI TGGCCA 1 cut(s) 945
BamHI GGATCC 1 cut(s) 175
BanI GGYRCC 1 cut(s) 919
BauI CACGAG 2 cut(s) 880, 1221
BbvI GCAGC 5 cut(s) 749, 968, 1189, 1273, 1309
BccI CCATC 3 cut(s) 55, 326, 821
BclI TGATCA 1 cut(s) 592
BcnI CCSGG 2 cut(s) 1258, 1319
BcoDI GTCTC 2 cut(s) 308, 765
BfaI CTAG 4 cut(s) 290, 371, 1142, 1326
BfmI CTRYAG 3 cut(s) 69, 906, 1380
BfrI CTTAAG 1 cut(s) 797
BglII AGATCT 1 cut(s) 793
BisI GCNGC 6 cut(s) 738, 957, 1178, 1262, 1323, 1383
BlsI GCNGC 6 cut(s) 739, 958, 1179, 1263, 1324, 1384
BmcAI AGTACT 1 cut(s) 299
Bme1390I CCNGG 2 cut(s) 1258, 1319
Bme18I GGWCC 4 cut(s) 113, 215, 286, 1151
BmeT110I CYCGRG 1 cut(s) 14
BmgT120I GGNCC 4 cut(s) 113, 215, 286, 1151
BmiI GGNNCC 5 cut(s) 177, 336, 490, 921, 1025
BmrFI CCNGG 2 cut(s) 1258, 1319
BmrI ACTGGG 2 cut(s) 128, 1029
BmsI GCATC 7 cut(s) 55, 373, 423, 811, 984, 1207, 1278
BmuI ACTGGG 2 cut(s) 128, 1029
BpuMI CCSGG 2 cut(s) 1258, 1319
BsaJI CCNNGG 2 cut(s) 700, 1317
BsaWI WCCGGW 1 cut(s) 922
BsaXI ACNNNNNCTCC 2 cut(s) 1340, 1370
Bsc4I CCNNNNNNNGG 6 cut(s) 344, 712, 952, 1019, 1160, 1358
Bse118I RCCGGY 1 cut(s) 1001
Bse1I ACTGG 3 cut(s) 122, 134, 1024
BseDI CCNNGG 2 cut(s) 700, 1317
BseGI GGATG 5 cut(s) 70, 364, 595, 826, 1222
BseLI CCNNNNNNNGG 6 cut(s) 344, 712, 952, 1019, 1160, 1358
BseMII CTCAG 2 cut(s) 344, 507
BseNI ACTGG 3 cut(s) 122, 134, 1024
BseRI GAGGAG 2 cut(s) 398, 731
BseXI GCAGC 5 cut(s) 749, 968, 1189, 1273, 1309
Bsh1236I CGCG 1 cut(s) 506
BshFI GGCC 3 cut(s) 705, 945, 1378
BshNI GGYRCC 1 cut(s) 919
BsiHKCI CYCGRG 1 cut(s) 14
BsiSI CCGG 5 cut(s) 923, 1002, 1257, 1319, 1359
BslI CCNNNNNNNGG 6 cut(s) 344, 712, 952, 1019, 1160, 1358
BsmAI GTCTC 2 cut(s) 308, 765
BsnI GGCC 3 cut(s) 705, 945, 1378
BsoBI CYCGRG 1 cut(s) 14
Bsp143I GATC 3 cut(s) 175, 592, 793
Bsp19I CCATGG 1 cut(s) 700
BspACI CCGC 1 cut(s) 755
BspANI GGCC 3 cut(s) 705, 945, 1378
BspCNI CTCAG 2 cut(s) 343, 506
BspFNI CGCG 1 cut(s) 506
BspHI TCATGA 1 cut(s) 534
BspLI GGNNCC 5 cut(s) 177, 336, 490, 921, 1025
BspMAI CTGCAG 1 cut(s) 1384
BspPI GGATC 2 cut(s) 170, 183
BspQI GCTCTTC 1 cut(s) 857
BspT107I GGYRCC 1 cut(s) 919
BspTI CTTAAG 1 cut(s) 797
BsrFI RCCGGY 1 cut(s) 1001
BsrI ACTGG 3 cut(s) 122, 134, 1024
BssAI RCCGGY 1 cut(s) 1001
BssECI CCNNGG 2 cut(s) 700, 1317
BssMI GATC 3 cut(s) 175, 592, 793
BssSI CACGAG 2 cut(s) 880, 1221
BssT1I CCWWGG 1 cut(s) 700
Bst2BI CACGAG 2 cut(s) 880, 1221
Bst4CI ACNGT 4 cut(s) 302, 353, 907, 1028
Bst6I CTCTTC 2 cut(s) 392, 857
BstAFI CTTAAG 1 cut(s) 797
BstC8I GCNNGC 5 cut(s) 723, 858, 1007, 1136, 1380
BstDEI CTNAG 3 cut(s) 330, 493, 614
BstDSI CCRYGG 1 cut(s) 700
BstEII GGTNACC 1 cut(s) 347
BstENI CCTNNNNNAGG 1 cut(s) 1158
BstF5I GGATG 5 cut(s) 70, 364, 595, 826, 1222
BstFNI CGCG 1 cut(s) 506
BstKTI GATC 3 cut(s) 178, 595, 796
BstMAI GTCTC 2 cut(s) 308, 765
BstMBI GATC 3 cut(s) 175, 592, 793
BstMWI GCNNNNNNNGC 1 cut(s) 152
BstNSI RCATGY 1 cut(s) 1303
BstPI GGTNACC 1 cut(s) 347
BstSCI CCNGG 2 cut(s) 1256, 1317
BstSFI CTRYAG 3 cut(s) 69, 906, 1380
BstUI CGCG 1 cut(s) 506
BstV1I GCAGC 5 cut(s) 749, 968, 1189, 1273, 1309
BstX2I RGATCY 2 cut(s) 175, 793
BstYI RGATCY 2 cut(s) 175, 793
BsuRI GGCC 3 cut(s) 705, 945, 1378
BtgI CCRYGG 1 cut(s) 700
BtgZI GCGATG 2 cut(s) 521, 1255
BtsCI GGATG 5 cut(s) 70, 364, 595, 826, 1222
BtsI GCAGTG 1 cut(s) 1264
BtsIMutI CAGTG 4 cut(s) 141, 239, 288, 1264
Cac8I GCNNGC 5 cut(s) 723, 858, 1007, 1136, 1380
CciI TCATGA 1 cut(s) 534
Cfr10I RCCGGY 1 cut(s) 1001
Cfr13I GGNCC 4 cut(s) 113, 215, 286, 1151
CseI GACGC 1 cut(s) 1127
Csp6I GTAC 3 cut(s) 119, 171, 298
CviQI GTAC 3 cut(s) 119, 171, 298
DdeI CTNAG 3 cut(s) 330, 493, 614
DpnI GATC 3 cut(s) 177, 594, 795
DpnII GATC 3 cut(s) 175, 592, 793
EaeI YGGCCR 1 cut(s) 943
Eam1104I CTCTTC 2 cut(s) 392, 857
EarI CTCTTC 2 cut(s) 392, 857
Eco130I CCWWGG 1 cut(s) 700
Eco147I AGGCCT 1 cut(s) 1378
Eco47I GGWCC 4 cut(s) 113, 215, 286, 1151
Eco88I CYCGRG 1 cut(s) 14
Eco91I GGTNACC 1 cut(s) 347
EcoNI CCTNNNNNAGG 1 cut(s) 1158
EcoO65I GGTNACC 1 cut(s) 347
EcoT14I CCWWGG 1 cut(s) 700
EcoT22I ATGCAT 1 cut(s) 789
ErhI CCWWGG 1 cut(s) 700
FbaI TGATCA 1 cut(s) 592
Fnu4HI GCNGC 6 cut(s) 738, 957, 1178, 1262, 1323, 1383
FokI GGATG 5 cut(s) 77, 351, 582, 833, 1229
Fsp4HI GCNGC 6 cut(s) 738, 957, 1178, 1262, 1323, 1383
FspBI CTAG 4 cut(s) 290, 371, 1142, 1326
GluI GCNGC 6 cut(s) 738, 957, 1178, 1262, 1323, 1383
HaeIII GGCC 3 cut(s) 705, 945, 1378
HapII CCGG 5 cut(s) 923, 1002, 1257, 1319, 1359
HgaI GACGC 1 cut(s) 1127
HincII GTYRAC 1 cut(s) 667
HindII GTYRAC 1 cut(s) 667
HindIII AAGCTT 3 cut(s) 611, 1007, 1237
HinfI GANTC 5 cut(s) 34, 140, 278, 326, 1313
HpaII CCGG 5 cut(s) 923, 1002, 1257, 1319, 1359
HphI GGTGA 4 cut(s) 359, 487, 755, 1175
Hpy166II GTNNAC 4 cut(s) 286, 667, 1151, 1312
Hpy188I TCNGA 7 cut(s) 11, 33, 103, 496, 993, 1032, 1332
Hpy188III TCNNGA 8 cut(s) 127, 332, 371, 394, 535, 1040, 1223, 1344
Hpy8I GTNNAC 4 cut(s) 286, 667, 1151, 1312
Hpy99I CGWCG 1 cut(s) 185
HpyAV CCTTC 7 cut(s) 228, 478, 505, 776, 1117, 1327, 1363
HpyCH4III ACNGT 4 cut(s) 302, 353, 907, 1028
HpyCH4IV ACGT 1 cut(s) 1251
HpyCH4V TGCA 8 cut(s) 21, 57, 461, 737, 787, 824, 1269, 1382
HpyF10VI GCNNNNNNNGC 1 cut(s) 152
HpyF3I CTNAG 3 cut(s) 330, 493, 614
HpySE526I ACGT 1 cut(s) 1251
Ksp22I TGATCA 1 cut(s) 592
Kzo9I GATC 3 cut(s) 175, 592, 793
LguI GCTCTTC 1 cut(s) 857
LmnI GCTCC 1 cut(s) 718
Lsp1109I GCAGC 5 cut(s) 749, 968, 1189, 1273, 1309
LweI GCATC 7 cut(s) 55, 373, 423, 811, 984, 1207, 1278
MaeI CTAG 4 cut(s) 290, 371, 1142, 1326
MaeII ACGT 1 cut(s) 1251
MaeIII GTNAC 2 cut(s) 198, 347
MalI GATC 3 cut(s) 177, 594, 795
MboI GATC 3 cut(s) 175, 592, 793
MflI RGATCY 2 cut(s) 175, 793
MlsI TGGCCA 1 cut(s) 945
MluCI AATT 9 cut(s) 27, 51, 148, 563, 889, 1197, 1201, 1211, 1285
MluNI TGGCCA 1 cut(s) 945
MlyI GAGTC 2 cut(s) 28, 1307
MmeI TCCRAC 1 cut(s) 1267
Mox20I TGGCCA 1 cut(s) 945
Mph1103I ATGCAT 1 cut(s) 789
MroXI GAANNNNTTC 1 cut(s) 930
MscI TGGCCA 1 cut(s) 945
MseI TTAA 5 cut(s) 581, 798, 842, 1200, 1390
MslI CAYNNNNRTG 1 cut(s) 774
Msp20I TGGCCA 1 cut(s) 945
MspCI CTTAAG 1 cut(s) 797
MspI CCGG 5 cut(s) 923, 1002, 1257, 1319, 1359
MspR9I CCNGG 2 cut(s) 1258, 1319
MvnI CGCG 1 cut(s) 506
MwoI GCNNNNNNNGC 1 cut(s) 152
NciI CCSGG 2 cut(s) 1258, 1319
NcoI CCATGG 1 cut(s) 700
NdeII GATC 3 cut(s) 175, 592, 793
NlaIV GGNNCC 5 cut(s) 177, 336, 490, 921, 1025
NmeAIII GCCGAG 1 cut(s) 766
NmuCI GTSAC 2 cut(s) 198, 347
NsiI ATGCAT 1 cut(s) 789
NspI RCATGY 1 cut(s) 1303
PagI TCATGA 1 cut(s) 534
PceI AGGCCT 1 cut(s) 1378
PciSI GCTCTTC 1 cut(s) 857
PdmI GAANNNNTTC 1 cut(s) 930
PfeI GAWTC 3 cut(s) 140, 278, 326
PflFI GACNNNGTC 2 cut(s) 353, 530
PkrI GCNGC 6 cut(s) 739, 958, 1179, 1263, 1324, 1384
PleI GAGTC 2 cut(s) 28, 1307
PpsI GAGTC 2 cut(s) 28, 1307
PshBI ATTAAT 1 cut(s) 1200
PsiI TTATAA 1 cut(s) 267
Psp1406I AACGTT 1 cut(s) 1251
PspEI GGTNACC 1 cut(s) 347
PspN4I GGNNCC 5 cut(s) 177, 336, 490, 921, 1025
PspPI GGNCC 4 cut(s) 113, 215, 286, 1151
PstI CTGCAG 1 cut(s) 1384
PsuI RGATCY 2 cut(s) 175, 793
PsyI GACNNNGTC 2 cut(s) 353, 530
RsaI GTAC 3 cut(s) 120, 172, 299
RsaNI GTAC 3 cut(s) 119, 171, 298
RseI CAYNNNNRTG 1 cut(s) 774
SapI GCTCTTC 1 cut(s) 857
SaqAI TTAA 5 cut(s) 581, 798, 842, 1200, 1390
SatI GCNGC 6 cut(s) 738, 957, 1178, 1262, 1323, 1383
Sau3AI GATC 3 cut(s) 175, 592, 793
Sau96I GGNCC 4 cut(s) 113, 215, 286, 1151
ScaI AGTACT 1 cut(s) 299
SchI GAGTC 2 cut(s) 28, 1307
ScrFI CCNGG 2 cut(s) 1258, 1319
SfaNI GCATC 7 cut(s) 55, 373, 423, 811, 984, 1207, 1278
SfcI CTRYAG 3 cut(s) 69, 906, 1380
SinI GGWCC 4 cut(s) 113, 215, 286, 1151
SmiMI CAYNNNNRTG 1 cut(s) 774
SmlI CTYRAG 1 cut(s) 797
SmoI CTYRAG 1 cut(s) 797
Sse9I AATT 9 cut(s) 27, 51, 148, 563, 889, 1197, 1201, 1211, 1285
SseBI AGGCCT 1 cut(s) 1378
SsiI CCGC 1 cut(s) 755
SspMI CTAG 4 cut(s) 290, 371, 1142, 1326
StuI AGGCCT 1 cut(s) 1378
StyD4I CCNGG 2 cut(s) 1256, 1317
StyI CCWWGG 1 cut(s) 700
TaaI ACNGT 4 cut(s) 302, 353, 907, 1028
TaiI ACGT 1 cut(s) 1254
TaqI TCGA 1 cut(s) 417
TasI AATT 9 cut(s) 27, 51, 148, 563, 889, 1197, 1201, 1211, 1285
TatI WGTACW 1 cut(s) 297
TfiI GAWTC 3 cut(s) 140, 278, 326
Tru1I TTAA 5 cut(s) 581, 798, 842, 1200, 1390
Tru9I TTAA 5 cut(s) 581, 798, 842, 1200, 1390
TscAI CASTG 4 cut(s) 141, 246, 288, 1271
TseFI GTSAC 2 cut(s) 198, 347
TseI GCWGC 6 cut(s) 737, 956, 1177, 1261, 1322, 1382
Tsp45I GTSAC 2 cut(s) 198, 347
TspDTI ATGAA 9 cut(s) 210, 324, 524, 551, 575, 592, 758, 1182, 1244
TspGWI ACGGA 2 cut(s) 188, 684
TspRI CASTG 4 cut(s) 141, 246, 288, 1271
Tth111I GACNNNGTC 2 cut(s) 353, 530
Vha464I CTTAAG 1 cut(s) 797
VpaK11BI GGWCC 4 cut(s) 113, 215, 286, 1151
VspI ATTAAT 1 cut(s) 1200
XagI CCTNNNNNAGG 1 cut(s) 1158
XapI RAATTY 1 cut(s) 563
XbaI TCTAGA 1 cut(s) 370
XceI RCATGY 1 cut(s) 1303
XmnI GAANNNNTTC 1 cut(s) 930
XspI CTAG 4 cut(s) 290, 371, 1142, 1326
ZrmI AGTACT 1 cut(s) 299
Zsp2I ATGCAT 1 cut(s) 789
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.