Rroxscaffold_7G00177040

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0005471
Physical Location & Seq
Forward (+)
16598224 .. 16599316
1093 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00177040.1

Sequence Viewer

Length: 1077 bp
ATGAAGAAATTAACCATCAAAGAATCTCAGGAACCTTTTGTTGGTGACCGTGTCATTGGGCATCCTCTAGAAAATGTTGAGGAGAGTATCTTGAAGAGTGAGAGTGTAGGCATCGAAGATTTTGAGGTTTTGAAGATTGTTGGGCAGGGTGGATTTGCAAAAGTATATCAGGTGAGGAAGAAGGGAACCTCGGAAATATACGCGATGAAAGTAATGCGAAAGGACAAGATCATGAAGAATAATCTTGCTGAATACATGAAATTTGAGAGGGATATATTAACAAAAGTTGATCATCCATTCATTGTCAAGCTTAGATACTCATTCCAAACCAAATACAGACTATATCTTGTGCTGGATTTTGTCAACGGAGGTCAGCTTTTCTTTCAACTCTACCACCATGGCCTTTTTTTGGAGGAGCTGGCACGAATATATGCAGCCGAGATTGTTTCTGCGGTGTCTCACCTTCATTCAAATGGCATAATGCATAGAGATCTTAACTCTGAAAATATCCTATTGGATGCAGATGGCCATGCCATTTTAACTGATTTTGGGCTTGCGAAGAGCTTTGACGAAAACACGAGGTCTAATTCTCTATGTGGAACTGTAGAATACATGGCACCGGAAATAGTTCAGGGAAATGGCCATGATAAGGCAGCAGATTGGTGGAGTGTGGGAGTTGTATTGTTTCGGATGCTTACCGGCAAGCTTCCTTTTACTGGGAACCGTCAGAAATGTCAAGATAAGATAGTAAAGGAGAAAATCAAGTTGCCAAGTTTTTTGTCAAGTGAAGCACATTCTCTGTTGAAAGGCTTGTTAGCGAAGGACACAAGCAAGCGCCTAGGTTGTGGACCTCTGGGAGGTGATGAAGTCAAGCAGCACAAATGGTTCAAGAAAATTAATTGGAAGAAATTGGATGCTCGTGAAATCAAACCGAGCTTCCCACCTAACCACTGCATTGCCAACATTGAGAATTACTGGACTGACACGCCACTTGTGGACTCCCCGGCTGCTAGTCCGAAGGCTTCGTCTGGAAATCCCTTCTCCGGCTTTAGTTATGTTAGGTCTGCAGCCTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006417 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008285 GO:0009266 GO:0009408 GO:0009409 GO:0009628 GO:0009651 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010608 GO:0010628 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032268 GO:0032270 GO:0034248 GO:0034250 GO:0035556 GO:0036211 GO:0042127 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045727 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051716 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0140096 GO:1901564 GO:2000112
KEGG Pathways
Metabolic & Signaling
ko01521 ko01522 ko04012 ko04066 ko04140 ko04150 ko04151 ko04152 ko04211 ko04212 ko04213 ko04350 ko04371 ko04666 ko04714 ko04910 ko04931 ko05165 ko05200 ko05205 ko05210 ko05212 ko05221 ko05224 ko05225 ko05226 ko05231 map01521 map01522 map04012 map04066 map04140 map04150 map04151 map04152 map04211 map04212 map04213 map04350 map04371 map04666 map04714 map04910 map04931 map05165 map05200 map05205 map05210 map05212 map05221 map05224 map05225 map05226 map05231
Pfam Domains
Protein Families
Pkinase Pkinase_C

Protein Analysis

358

Amino Acids

40.36

Weight (kDa)

8.98

Isoelectric Point (pI)

32.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 41 - 296 2.3e-72 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 43 - 279 6.9e-34 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 115 - 196 1.9e-06 ABC1 atypical kinase-like domain
Kinase-like PF14531 137 - 279 7.8e-09 Kinase-like
Pkinase_fungal PF17667 148 - 229 2.2e-08 Fungal protein kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 616
AccII CGCG 1 cut(s) 203
AciI CCGC 1 cut(s) 452
AcoI YGGCCR 2 cut(s) 526, 640
AcsI RAATTY 1 cut(s) 260
AfiI CCNNNNNNNGG 6 cut(s) 41, 409, 649, 716, 857, 1043
AgsI TTSAA 6 cut(s) 94, 133, 386, 471, 805, 889
AjuI GAANNNNNNNTTGG 2 cut(s) 24, 56
AluBI AGCT 6 cut(s) 310, 376, 418, 564, 706, 936
AluI AGCT 6 cut(s) 310, 376, 418, 564, 706, 936
Alw26I GTCTC 1 cut(s) 462
AoxI GGCC 3 cut(s) 400, 526, 640
ApeKI GCWGC 5 cut(s) 434, 653, 874, 1007, 1067
ApoI RAATTY 1 cut(s) 260
ArsI GACNNNNNNTTYG 2 cut(s) 1010, 1042
AseI ATTAAT 1 cut(s) 897
Asp700I GAANNNNTTC 1 cut(s) 627
AspA2I CCTAGG 1 cut(s) 838
AspLEI GCGC 1 cut(s) 837
AspS9I GGNCC 1 cut(s) 848
AsuC2I CCSGG 1 cut(s) 1004
AsuHPI GGTGA 4 cut(s) 56, 184, 452, 872
AvaII GGWCC 1 cut(s) 848
AvrII CCTAGG 1 cut(s) 838
BalI TGGCCA 2 cut(s) 528, 642
BanI GGYRCC 1 cut(s) 616
BauI CACGAG 2 cut(s) 577, 918
BbvI GCAGC 4 cut(s) 446, 665, 886, 994
BccI CCATC 2 cut(s) 23, 518
BclI TGATCA 1 cut(s) 289
BcnI CCSGG 1 cut(s) 1004
BcoDI GTCTC 1 cut(s) 462
BfaI CTAG 3 cut(s) 68, 839, 1011
BfmI CTRYAG 2 cut(s) 603, 1065
BfoI RGCGCY 1 cut(s) 838
BglII AGATCT 1 cut(s) 490
BisI GCNGC 5 cut(s) 435, 654, 875, 1008, 1068
BlnI CCTAGG 1 cut(s) 838
BlsI GCNGC 5 cut(s) 436, 655, 876, 1009, 1069
Bme1390I CCNGG 1 cut(s) 1004
Bme18I GGWCC 1 cut(s) 848
BmgT120I GGNCC 1 cut(s) 848
BmiI GGNNCC 4 cut(s) 33, 187, 618, 722
BmrFI CCNGG 1 cut(s) 1004
BmrI ACTGGG 1 cut(s) 726
BmsI GCATC 5 cut(s) 70, 120, 508, 681, 904
BmuI ACTGGG 1 cut(s) 726
BpuMI CCSGG 1 cut(s) 1004
BsaJI CCNNGG 4 cut(s) 189, 397, 838, 1002
BsaWI WCCGGW 1 cut(s) 619
Bsc4I CCNNNNNNNGG 6 cut(s) 41, 409, 649, 716, 857, 1043
Bse118I RCCGGY 1 cut(s) 698
Bse1I ACTGG 2 cut(s) 721, 980
Bse3DI GCAATG 1 cut(s) 954
BseDI CCNNGG 4 cut(s) 189, 397, 838, 1002
BseGI GGATG 5 cut(s) 61, 292, 523, 696, 919
BseLI CCNNNNNNNGG 6 cut(s) 41, 409, 649, 716, 857, 1043
BseMI GCAATG 1 cut(s) 954
BseMII CTCAG 1 cut(s) 41
BseNI ACTGG 2 cut(s) 721, 980
BseRI GAGGAG 2 cut(s) 95, 428
BseXI GCAGC 4 cut(s) 446, 665, 886, 994
Bsh1236I CGCG 1 cut(s) 203
BshFI GGCC 3 cut(s) 402, 528, 642
BshNI GGYRCC 1 cut(s) 616
BsiSI CCGG 4 cut(s) 620, 699, 1004, 1044
BslI CCNNNNNNNGG 6 cut(s) 41, 409, 649, 716, 857, 1043
BsmAI GTCTC 1 cut(s) 462
BsnI GGCC 3 cut(s) 402, 528, 642
Bsp143I GATC 3 cut(s) 228, 289, 490
Bsp19I CCATGG 1 cut(s) 397
BspACI CCGC 1 cut(s) 452
BspANI GGCC 3 cut(s) 402, 528, 642
BspCNI CTCAG 1 cut(s) 40
BspFNI CGCG 1 cut(s) 203
BspHI TCATGA 1 cut(s) 231
BspLI GGNNCC 4 cut(s) 33, 187, 618, 722
BspMAI CTGCAG 1 cut(s) 1069
BspQI GCTCTTC 1 cut(s) 554
BspT107I GGYRCC 1 cut(s) 616
BsrDI GCAATG 1 cut(s) 954
BsrFI RCCGGY 1 cut(s) 698
BsrI ACTGG 2 cut(s) 721, 980
BssAI RCCGGY 1 cut(s) 698
BssECI CCNNGG 4 cut(s) 189, 397, 838, 1002
BssMI GATC 3 cut(s) 228, 289, 490
BssSI CACGAG 2 cut(s) 577, 918
BssT1I CCWWGG 2 cut(s) 397, 838
Bst2BI CACGAG 2 cut(s) 577, 918
Bst4CI ACNGT 3 cut(s) 50, 604, 725
Bst6I CTCTTC 2 cut(s) 89, 554
BstC8I GCNNGC 4 cut(s) 420, 555, 704, 833
BstDEI CTNAG 2 cut(s) 27, 311
BstDSI CCRYGG 1 cut(s) 397
BstEII GGTNACC 1 cut(s) 44
BstENI CCTNNNNNAGG 1 cut(s) 855
BstF5I GGATG 5 cut(s) 61, 292, 523, 696, 919
BstFNI CGCG 1 cut(s) 203
BstH2I RGCGCY 1 cut(s) 838
BstHHI GCGC 1 cut(s) 837
BstKTI GATC 3 cut(s) 231, 292, 493
BstMAI GTCTC 1 cut(s) 462
BstMBI GATC 3 cut(s) 228, 289, 490
BstPI GGTNACC 1 cut(s) 44
BstSCI CCNGG 1 cut(s) 1002
BstSFI CTRYAG 2 cut(s) 603, 1065
BstUI CGCG 1 cut(s) 203
BstV1I GCAGC 4 cut(s) 446, 665, 886, 994
BstX2I RGATCY 1 cut(s) 490
BstYI RGATCY 1 cut(s) 490
BsuRI GGCC 3 cut(s) 402, 528, 642
BtgI CCRYGG 1 cut(s) 397
BtgZI GCGATG 1 cut(s) 218
BtsCI GGATG 5 cut(s) 61, 292, 523, 696, 919
BtsI GCAGTG 1 cut(s) 949
BtsIMutI CAGTG 1 cut(s) 949
Cac8I GCNNGC 4 cut(s) 420, 555, 704, 833
CciI TCATGA 1 cut(s) 231
CfoI GCGC 1 cut(s) 837
Cfr10I RCCGGY 1 cut(s) 698
Cfr13I GGNCC 1 cut(s) 848
CviAII CATG 6 cut(s) 232, 256, 398, 530, 613, 644
DdeI CTNAG 2 cut(s) 27, 311
DpnI GATC 3 cut(s) 230, 291, 492
DpnII GATC 3 cut(s) 228, 289, 490
EaeI YGGCCR 2 cut(s) 526, 640
Eam1104I CTCTTC 2 cut(s) 89, 554
EarI CTCTTC 2 cut(s) 89, 554
Eco130I CCWWGG 2 cut(s) 397, 838
Eco47I GGWCC 1 cut(s) 848
Eco91I GGTNACC 1 cut(s) 44
EcoNI CCTNNNNNAGG 1 cut(s) 855
EcoO65I GGTNACC 1 cut(s) 44
EcoT14I CCWWGG 2 cut(s) 397, 838
EcoT22I ATGCAT 1 cut(s) 486
ErhI CCWWGG 2 cut(s) 397, 838
FaeI CATG 6 cut(s) 235, 259, 401, 533, 616, 647
FatI CATG 6 cut(s) 231, 255, 397, 529, 612, 643
FbaI TGATCA 1 cut(s) 289
Fnu4HI GCNGC 5 cut(s) 435, 654, 875, 1008, 1068
FokI GGATG 5 cut(s) 48, 279, 530, 703, 926
Fsp4HI GCNGC 5 cut(s) 435, 654, 875, 1008, 1068
FspBI CTAG 3 cut(s) 68, 839, 1011
GlaI GCGC 1 cut(s) 836
GluI GCNGC 5 cut(s) 435, 654, 875, 1008, 1068
HaeII RGCGCY 1 cut(s) 838
HaeIII GGCC 3 cut(s) 402, 528, 642
HapII CCGG 4 cut(s) 620, 699, 1004, 1044
HhaI GCGC 1 cut(s) 837
Hin1II CATG 6 cut(s) 235, 259, 401, 533, 616, 647
Hin6I GCGC 1 cut(s) 835
HinP1I GCGC 1 cut(s) 835
HincII GTYRAC 1 cut(s) 364
HindII GTYRAC 1 cut(s) 364
HindIII AAGCTT 2 cut(s) 308, 704
HinfI GANTC 2 cut(s) 23, 998
HpaII CCGG 4 cut(s) 620, 699, 1004, 1044
HphI GGTGA 4 cut(s) 56, 184, 452, 872
Hpy166II GTNNAC 3 cut(s) 364, 848, 997
Hpy188I TCNGA 5 cut(s) 193, 502, 690, 729, 1017
Hpy188III TCNNGA 8 cut(s) 29, 68, 91, 232, 737, 889, 920, 1029
Hpy8I GTNNAC 3 cut(s) 364, 848, 997
HpyAV CCTTC 5 cut(s) 175, 473, 814, 1012, 1048
HpyCH4III ACNGT 3 cut(s) 50, 604, 725
HpyCH4V TGCA 6 cut(s) 158, 434, 484, 521, 954, 1067
HpyF3I CTNAG 2 cut(s) 27, 311
Hsp92II CATG 6 cut(s) 235, 259, 401, 533, 616, 647
HspAI GCGC 1 cut(s) 835
Ksp22I TGATCA 1 cut(s) 289
Kzo9I GATC 3 cut(s) 228, 289, 490
LguI GCTCTTC 1 cut(s) 554
LmnI GCTCC 1 cut(s) 415
Lsp1109I GCAGC 4 cut(s) 446, 665, 886, 994
LweI GCATC 5 cut(s) 70, 120, 508, 681, 904
MaeI CTAG 3 cut(s) 68, 839, 1011
MaeIII GTNAC 1 cut(s) 44
MalI GATC 3 cut(s) 230, 291, 492
MboI GATC 3 cut(s) 228, 289, 490
MboII GAAGA 8 cut(s) 16, 106, 128, 145, 190, 247, 571, 916
MflI RGATCY 1 cut(s) 490
MlsI TGGCCA 2 cut(s) 528, 642
MluCI AATT 7 cut(s) 8, 260, 586, 894, 898, 908, 970
MluNI TGGCCA 2 cut(s) 528, 642
MlyI GAGTC 1 cut(s) 992
Mox20I TGGCCA 2 cut(s) 528, 642
Mph1103I ATGCAT 1 cut(s) 486
MroXI GAANNNNTTC 1 cut(s) 627
MscI TGGCCA 2 cut(s) 528, 642
MseI TTAA 6 cut(s) 11, 278, 495, 539, 897, 1075
MslI CAYNNNNRTG 1 cut(s) 471
Msp20I TGGCCA 2 cut(s) 528, 642
MspI CCGG 4 cut(s) 620, 699, 1004, 1044
MspR9I CCNGG 1 cut(s) 1004
MvnI CGCG 1 cut(s) 203
NciI CCSGG 1 cut(s) 1004
NcoI CCATGG 1 cut(s) 397
NdeII GATC 3 cut(s) 228, 289, 490
NlaIII CATG 6 cut(s) 235, 259, 401, 533, 616, 647
NlaIV GGNNCC 4 cut(s) 33, 187, 618, 722
NmeAIII GCCGAG 1 cut(s) 463
NmuCI GTSAC 1 cut(s) 44
NsiI ATGCAT 1 cut(s) 486
PagI TCATGA 1 cut(s) 231
PciSI GCTCTTC 1 cut(s) 554
PdmI GAANNNNTTC 1 cut(s) 627
PfeI GAWTC 1 cut(s) 23
PflFI GACNNNGTC 1 cut(s) 50
PkrI GCNGC 5 cut(s) 436, 655, 876, 1009, 1069
PleI GAGTC 1 cut(s) 992
PpsI GAGTC 1 cut(s) 992
PshBI ATTAAT 1 cut(s) 897
PspEI GGTNACC 1 cut(s) 44
PspN4I GGNNCC 4 cut(s) 33, 187, 618, 722
PspPI GGNCC 1 cut(s) 848
PstI CTGCAG 1 cut(s) 1069
PsuI RGATCY 1 cut(s) 490
PsyI GACNNNGTC 1 cut(s) 50
RseI CAYNNNNRTG 1 cut(s) 471
SapI GCTCTTC 1 cut(s) 554
SaqAI TTAA 6 cut(s) 11, 278, 495, 539, 897, 1075
SatI GCNGC 5 cut(s) 435, 654, 875, 1008, 1068
Sau3AI GATC 3 cut(s) 228, 289, 490
Sau96I GGNCC 1 cut(s) 848
SchI GAGTC 1 cut(s) 992
ScrFI CCNGG 1 cut(s) 1004
SfaNI GCATC 5 cut(s) 70, 120, 508, 681, 904
SfcI CTRYAG 2 cut(s) 603, 1065
SinI GGWCC 1 cut(s) 848
SmiMI CAYNNNNRTG 1 cut(s) 471
Sse9I AATT 7 cut(s) 8, 260, 586, 894, 898, 908, 970
SsiI CCGC 1 cut(s) 452
SspMI CTAG 3 cut(s) 68, 839, 1011
StyD4I CCNGG 1 cut(s) 1002
StyI CCWWGG 2 cut(s) 397, 838
TaaI ACNGT 3 cut(s) 50, 604, 725
TaqI TCGA 1 cut(s) 114
TasI AATT 7 cut(s) 8, 260, 586, 894, 898, 908, 970
TfiI GAWTC 1 cut(s) 23
Tru1I TTAA 6 cut(s) 11, 278, 495, 539, 897, 1075
Tru9I TTAA 6 cut(s) 11, 278, 495, 539, 897, 1075
TscAI CASTG 1 cut(s) 956
TseFI GTSAC 1 cut(s) 44
TseI GCWGC 5 cut(s) 434, 653, 874, 1007, 1067
Tsp45I GTSAC 1 cut(s) 44
TspDTI ATGAA 7 cut(s) 17, 221, 248, 272, 289, 455, 879
TspGWI ACGGA 1 cut(s) 381
TspRI CASTG 1 cut(s) 956
Tth111I GACNNNGTC 1 cut(s) 50
VpaK11BI GGWCC 1 cut(s) 848
VspI ATTAAT 1 cut(s) 897
XagI CCTNNNNNAGG 1 cut(s) 855
XapI RAATTY 1 cut(s) 260
XbaI TCTAGA 1 cut(s) 67
XmaJI CCTAGG 1 cut(s) 838
XmnI GAANNNNTTC 1 cut(s) 627
XspI CTAG 3 cut(s) 68, 839, 1011
Zsp2I ATGCAT 1 cut(s) 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.