MD04G1201400.v1.1

Belongs to the formin-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0005682
Physical Location & Seq
Forward (+)
28773880 .. 28777386
3507 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1201400.v1.1.491

Sequence Viewer

Length: 2790 bp
ATGGCATATATTTTCTCTCAGATTCTCCTCCTTATGATCTTTTTCATCATATCTTTTCTCCTGTCATTACACAATGCACCAGAGGAATGCTTCAATGTTCATGGCCTGCAACACCACCTGCATTTCATGGAGGACAATGCCGAGGCTCCCCAAAACTTACAGATTCACAGAGTTTCCGGGGAAGATGAAGAGAATGCAGTTCCGGTTGTCGAAAAGTTCAGAGCTTTACTCGGACTGAACAGCTTCCACAAGCGAACGCCGTCAGCTGCCGACAGTGGAAATGTTTCTCCTTCTCCTTCTCCGTCGCCCTACAGTGAAGCTGAAGCTCCGAGTCCAGCTCCCGCTCCCGCTCCTGCTCCTTCTCCGGCGCCCCACATCCATGCGCATTCGCATCATCCGCATCGCCTGCGCTCAACCTCAAAGCCTCAAGACATTCACAGAGAAGACAGAGGCGGAATGGCGAGGATACTTGTACCGGTTCTTGTTTCTGCGGGAGCTGCGGTTGTGATTTGTGTGCTCGGCATCGTTTGGGGTTGCAGGAAGCAGAGGAAACATAAGAAGAAACCCACGAGCGTGATTTCGGTTTTCGGCCAAAACGGAGGAGCTAAATCACCAAGCAAGGTGAAACTAAATGCTGCCACCACCACCACTAGTTCTGATCTCATATATCCTGATTCGTTAGGGTTGGATTTAGAGCAGCAACAGCACCATATTTCTTGTTCCAAAGAAACTTGCGAAACTGTAATTACGTCACGAAATCATACATTGCTTGAGAGGGAAGAAACAAATCAACAAGTGATGAAATCGGAATTCGACGATGCTTGTAGTAGTTCTTCCACTGGGGAAATTATGTCTGTTCACGAGGATATAATTGCAGAGGCAGTGAAATATGCCTCTGATTGTGCTAATTCCGCAACCGGTGACAAAATTATTCCTGTTGATTCGTATTCTTCCGATGATGAATCTTTCCATTCTTTCGACAATTCGAATTCATCCAACATCCGCCTTTCAAATGCCTCAGCTGGTAGCCTAGGTGACTCATCAGATGTTCTCTCCACAAATGTGTCGAACATAGAACCTTGTTTAGAAGCACCTTCTATAAACTTACCAGATCAACCAGAAACCATAATCCGAAATAAAGAATCCAAACTTCTGATTGCACCGTGCTCTTCCAATTGCGAAAAGAATGTCAACGCTCCTCCAGCACCACCTCCTCCACCCCCACCACCAATCAAACATTTTCCTCATTTTTATTCTTCTCCCAGATCAACTAGAATTGCATCGAAAGCTTCGTGCTCGTCTTCTACATTGCAAAATCTATCACCGCCAAGAAAATCAGGTTCTTCTTGTGGATCAAATCAAACCCCCGAAGGCGGATTTTCTGTTTCGCCTCAGAACTCTTCCAGACCTTCAGAAACTCCACCTCCAATTCCTCCACCACCCTGCCCACCTCCCTTTTCGAAAGCGAATAATAGCTTTTTGAAAGGTCCTCCTCCTCCACCCCTCCCTCAGGCTACACCACTGGGGAAAGATGGAACTCCCCTGCCTAAACTGAAGCCGTTGCATTGGGACAAAGTCAGAGCTGCACCGGATCATTCTATGGTGTGGGACAAACTGAGATCAAGCTCGTTCGAGCTGGATGAGGAAATGATTGAATCGCTTTTCGGATACAATCTACAAGGCTCAATGAAGAATGATGAAGCAAAGAGCAAGAGCCCTTCTCCAAGCAAGCATGTTCTTGAGCCGAAAAGACTACAGAACATAACCATACTCTCGAAAGCCCTGAATTCAACCGCTGATCAAGTATGTGAAGCACTAGTGCAAGGAAATGGCTTGCGCCTACAACAACTGGAAGCGCTCGTGAGGATGGAACTCACCAAGGAGGAAGAGGGTAAGCTCACCGGCTACAAAGGAGACATCAATGAACTCGGGTCAGCGGAGAAGTTTGTGAAGGCGGTGCTTAGAGTACCTTTCGCTTTCCAACGAGTTGAAGCAATGCTTTACAGAGAAACGTTTGAAGATGAGGTGGTTCACCTTAGGAACTCCTTTTCAGTGCTAGAGGAGGCCTGTAAGGAACTGAGGTCAAGCAGGCTCTTCTTGAAGCTACTCGAAGCGGTGTTGAAAACAGGAAACCGGATGAATGTTGGAACGATCCGAGGAGGTGCCAAAGCATTCAAGCTAGACACTCTCCTCAAGCTCTCCGATGTAAAAGGAACCGATGGAAAAACCACATTGCTACATTTTGTTGTGCAAGAAATAATACGGACAGAAGGGATTCGAGTTTCGGACAGCATCATGGGGAAGATCAGCCAGAAGAACAAAACCAAAACCCTAGAAGAGAAAGAAGAAGACTACAGAAGAATGGGGCTTGACCTAGTTTCCGGCCTCAGCACCGAGCTCTACAACGCGAAGAAAACAGCCACATTGGACTTGGATGTTCTTGCAAGCAGTGTATCAAATCTCTCCGATGGAATGGAGAACATCAAACATCTCATACACAAGGAGTTGTGCATGGATGAGAACAGTGGTAATTTGGTCAATTCGATGAAATCGTTCATATGTTATGCAGAGGAACATTTGAGAGAGTTGCAGGGTGATGAGAGTAGGGTTCTGTCACAGGTGAAGGAGATAACAGAATATTTTCACGGGAATGTGAGCAAGGAAGAAGCGAATCCGCTTCGGATTTTCGTGATTGTTAGGGACTTTTTGGGAATGTTGGATCATGTTTGTAAAGAGCTTAGAAGCTCAAAAGCTCCACGCACTCCAAATCCTCTGGCTCCATTCAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0008092
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FH2

Protein Analysis

930

Amino Acids

101.84

Weight (kDa)

6.43

Isoelectric Point (pI)

57.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FH2 PF02181 511 - 906 7.3e-106 Formin Homology 2 Domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 126
Acc16I TGCGCA 1 cut(s) 384
Acc36I ACCTGC 1 cut(s) 126
AccB1I GGYRCC 2 cut(s) 367, 2162
AccBSI CCGCTC 2 cut(s) 344, 350
AccII CGCG 1 cut(s) 2408
AclI AACGTT 1 cut(s) 2012
AclWI GGATC 4 cut(s) 1360, 1599, 2146, 2727
AcoI YGGCCR 1 cut(s) 589
AcsI RAATTY 3 cut(s) 809, 988, 1786
AcuI CTGAAG 3 cut(s) 342, 1395, 1574
AcyI GRCGYC 1 cut(s) 368
AfaI GTAC 2 cut(s) 474, 1968
AfeI AGCGCT 1 cut(s) 1857
AfiI CCNNNNNNNGG 2 cut(s) 1373, 1510
AgeI ACCGGT 2 cut(s) 475, 917
AhlI ACTAGT 2 cut(s) 650, 1816
AleI CACNNNNGTG 2 cut(s) 572, 1061
Alw21I GWGCWC 4 cut(s) 519, 1169, 1298, 2400
Alw26I GTCTC 1 cut(s) 1908
AlwI GGATC 4 cut(s) 1360, 1599, 2146, 2727
Ama87I CYCGRG 1 cut(s) 1928
Aor51HI AGCGCT 1 cut(s) 1857
AoxI GGCC 4 cut(s) 103, 589, 2064, 2383
ApeKI GCWGC 5 cut(s) 266, 497, 635, 697, 1583
ApoI RAATTY 3 cut(s) 809, 988, 1786
ArsI GACNNNNNNTTYG 2 cut(s) 1173, 1205
AsiGI ACCGGT 2 cut(s) 475, 917
Asp700I GAANNNNTTC 5 cut(s) 242, 283, 2274, 2552, 2640
AspA2I CCTAGG 1 cut(s) 1030
AspLEI GCGC 5 cut(s) 370, 385, 411, 1839, 1858
AspS9I GGNCC 1 cut(s) 1487
AsuC2I CCSGG 1 cut(s) 178
AsuII TTCGAA 2 cut(s) 986, 1460
AvaI CYCGRG 1 cut(s) 1928
AvaII GGWCC 1 cut(s) 1487
AvrII CCTAGG 1 cut(s) 1030
AxyI CCTNAGG 2 cut(s) 1509, 2036
BanI GGYRCC 2 cut(s) 367, 2162
BanII GRGCYC 2 cut(s) 1718, 2400
BarI GAAGNNNNNNTAC 2 cut(s) 817, 849
BauI CACGAG 3 cut(s) 568, 860, 1859
BbsI GAAGAC 3 cut(s) 450, 1293, 2355
Bbv12I GWGCWC 4 cut(s) 519, 1169, 1298, 2400
BbvCI CCTCAGC 2 cut(s) 1018, 2387
BbvI GCAGC 5 cut(s) 253, 484, 622, 709, 1570
BccI CCATC 4 cut(s) 1526, 1861, 2213, 2462
BceAI ACGGC 2 cut(s) 244, 1543
BciVI GTATCC 2 cut(s) 459, 1661
BclI TGATCA 1 cut(s) 1798
BcnI CCSGG 1 cut(s) 178
BcoDI GTCTC 1 cut(s) 1908
BcuI ACTAGT 2 cut(s) 650, 1816
BfaI CTAG 8 cut(s) 651, 1031, 1272, 1817, 2057, 2180, 2333, 2375
BfmI CTRYAG 3 cut(s) 310, 1754, 2353
BfoI RGCGCY 2 cut(s) 371, 1859
BfuAI ACCTGC 1 cut(s) 126
BfuI GTATCC 2 cut(s) 459, 1661
BisI GCNGC 5 cut(s) 267, 498, 636, 698, 1584
BlnI CCTAGG 1 cut(s) 1030
BlsI GCNGC 5 cut(s) 268, 499, 637, 699, 1585
Bme1390I CCNGG 1 cut(s) 178
Bme18I GGWCC 1 cut(s) 1487
BmeT110I CYCGRG 1 cut(s) 1928
BmgT120I GGNCC 1 cut(s) 1487
BmiI GGNNCC 5 cut(s) 147, 369, 2164, 2215, 2778
BmrFI CCNGG 1 cut(s) 178
BmrI ACTGGG 2 cut(s) 849, 1532
BmsI GCATC 6 cut(s) 400, 409, 531, 808, 1289, 2301
BmuI ACTGGG 2 cut(s) 849, 1532
BpiI GAAGAC 3 cut(s) 450, 1293, 2355
BplI GAGNNNNNCTC 6 cut(s) 213, 245, 322, 354, 1705, 1737
BpmI CTGGAG 1 cut(s) 1185
Bpu10I CCTNAGC 2 cut(s) 1018, 2387
Bpu14I TTCGAA 2 cut(s) 986, 1460
BpuEI CTTGAG 4 cut(s) 411, 791, 1760, 2177
BpuMI CCSGG 1 cut(s) 178
BsaHI GRCGYC 1 cut(s) 368
BsaJI CCNNGG 5 cut(s) 141, 177, 1030, 1878, 2155
BsaWI WCCGGW 5 cut(s) 202, 475, 917, 1588, 2133
BsaXI ACNNNNNCTCC 4 cut(s) 486, 516, 1408, 1438
Bsc4I CCNNNNNNNGG 2 cut(s) 1373, 1510
Bse118I RCCGGY 3 cut(s) 475, 917, 1901
Bse1I ACTGG 3 cut(s) 844, 1527, 1854
Bse21I CCTNAGG 2 cut(s) 1509, 2036
Bse3DI GCAATG 4 cut(s) 764, 1307, 2001, 2231
BseDI CCNNGG 5 cut(s) 141, 177, 1030, 1878, 2155
BseGI GGATG 9 cut(s) 375, 394, 992, 999, 1645, 1872, 2142, 2440, 2521
BseLI CCNNNNNNNGG 2 cut(s) 1373, 1510
BseMI GCAATG 4 cut(s) 764, 1307, 2001, 2231
BseMII CTCAG 7 cut(s) 32, 1032, 1406, 1523, 1607, 2069, 2401
BseNI ACTGG 3 cut(s) 844, 1527, 1854
BseRI GAGGAG 9 cut(s) 17, 615, 1188, 1203, 1482, 1485, 2075, 2172, 2180
BseXI GCAGC 5 cut(s) 253, 484, 622, 709, 1570
BsgI GTGCAG 1 cut(s) 1569
Bsh1236I CGCG 1 cut(s) 2408
BshFI GGCC 4 cut(s) 105, 591, 2066, 2385
BshNI GGYRCC 2 cut(s) 367, 2162
BshTI ACCGGT 2 cut(s) 475, 917
BsiHKAI GWGCWC 4 cut(s) 519, 1169, 1298, 2400
BsiHKCI CYCGRG 1 cut(s) 1928
BsiSI CCGG 9 cut(s) 177, 203, 365, 476, 918, 1589, 1902, 2134, 2382
BslFI GGGAC 3 cut(s) 1583, 1622, 2714
BslI CCNNNNNNNGG 2 cut(s) 1373, 1510
BsmAI GTCTC 1 cut(s) 1908
BsmFI GGGAC 3 cut(s) 1583, 1622, 2714
BsmI GAATGC 4 cut(s) 92, 199, 385, 2171
BsnI GGCC 4 cut(s) 105, 591, 2066, 2385
BsoBI CYCGRG 1 cut(s) 1928
Bsp119I TTCGAA 2 cut(s) 986, 1460
Bsp1286I GDGCHC 5 cut(s) 519, 1169, 1298, 1718, 2400
BspANI GGCC 4 cut(s) 105, 591, 2066, 2385
BspCNI CTCAG 7 cut(s) 31, 1031, 1405, 1522, 1608, 2070, 2400
BspFNI CGCG 1 cut(s) 2408
BspLI GGNNCC 5 cut(s) 147, 369, 2164, 2215, 2778
BspMI ACCTGC 1 cut(s) 126
BspPI GGATC 4 cut(s) 1360, 1599, 2146, 2727
BspQI GCTCTTC 2 cut(s) 1174, 2099
BspT104I TTCGAA 2 cut(s) 986, 1460
BspT107I GGYRCC 2 cut(s) 367, 2162
BsrBI CCGCTC 2 cut(s) 344, 350
BsrDI GCAATG 4 cut(s) 764, 1307, 2001, 2231
BsrFI RCCGGY 3 cut(s) 475, 917, 1901
BsrI ACTGG 3 cut(s) 844, 1527, 1854
BssAI RCCGGY 3 cut(s) 475, 917, 1901
BssECI CCNNGG 5 cut(s) 141, 177, 1030, 1878, 2155
BssNI GRCGYC 1 cut(s) 368
BssSI CACGAG 3 cut(s) 568, 860, 1859
BssT1I CCWWGG 2 cut(s) 1030, 1878
Bst2BI CACGAG 3 cut(s) 568, 860, 1859
Bst4CI ACNGT 5 cut(s) 275, 314, 742, 1164, 2525
Bst6I CTCTTC 6 cut(s) 183, 1174, 1405, 1881, 2099, 2331
BstACI GRCGYC 1 cut(s) 368
BstAPI GCANNNNNTGC 1 cut(s) 406
BstBI TTCGAA 2 cut(s) 986, 1460
BstC8I GCNNGC 6 cut(s) 107, 407, 1730, 1835, 2090, 2446
BstENI CCTNNNNNAGG 1 cut(s) 1508
BstF5I GGATG 9 cut(s) 375, 394, 992, 999, 1645, 1872, 2142, 2440, 2521
BstFNI CGCG 1 cut(s) 2408
BstH2I RGCGCY 2 cut(s) 371, 1859
BstHHI GCGC 5 cut(s) 370, 385, 411, 1839, 1858
BstMAI GTCTC 1 cut(s) 1908
BstMWI GCNNNNNNNGC 7 cut(s) 397, 406, 497, 703, 911, 1202, 1286
BstNSI RCATGY 1 cut(s) 1736
BstSCI CCNGG 1 cut(s) 176
BstSFI CTRYAG 3 cut(s) 310, 1754, 2353
BstUI CGCG 1 cut(s) 2408
BstV1I GCAGC 5 cut(s) 253, 484, 622, 709, 1570
BstV2I GAAGAC 3 cut(s) 450, 1293, 2355
Bsu36I CCTNAGG 2 cut(s) 1509, 2036
BsuI GTATCC 2 cut(s) 459, 1661
BsuRI GGCC 4 cut(s) 105, 591, 2066, 2385
BtgZI GCGATG 1 cut(s) 386
BtsCI GGATG 9 cut(s) 375, 394, 992, 999, 1645, 1872, 2142, 2440, 2521
BtsI GCAGTG 2 cut(s) 888, 2455
BtsIMutI CAGTG 8 cut(s) 280, 319, 837, 888, 1520, 2058, 2455, 2530
BveI ACCTGC 1 cut(s) 126
Cac8I GCNNGC 6 cut(s) 107, 407, 1730, 1835, 2090, 2446
CfoI GCGC 5 cut(s) 370, 385, 411, 1839, 1858
Cfr10I RCCGGY 3 cut(s) 475, 917, 1901
Cfr13I GGNCC 1 cut(s) 1487
Csp6I GTAC 2 cut(s) 473, 1967
CspAI ACCGGT 2 cut(s) 475, 917
CviAII CATG 7 cut(s) 101, 127, 380, 1733, 2296, 2512, 2723
CviQI GTAC 2 cut(s) 473, 1967
DinI GGCGCC 1 cut(s) 369
EaeI YGGCCR 1 cut(s) 589
Eam1104I CTCTTC 6 cut(s) 183, 1174, 1405, 1881, 2099, 2331
EarI CTCTTC 6 cut(s) 183, 1174, 1405, 1881, 2099, 2331
EciI GGCGGA 3 cut(s) 468, 992, 1389
Ecl136II GAGCTC 1 cut(s) 2398
Eco130I CCWWGG 2 cut(s) 1030, 1878
Eco147I AGGCCT 1 cut(s) 2066
Eco24I GRGCYC 2 cut(s) 1718, 2400
Eco47I GGWCC 1 cut(s) 1487
Eco47III AGCGCT 1 cut(s) 1857
Eco53kI GAGCTC 1 cut(s) 2398
Eco57I CTGAAG 3 cut(s) 342, 1395, 1574
Eco81I CCTNAGG 2 cut(s) 1509, 2036
Eco88I CYCGRG 1 cut(s) 1928
EcoICRI GAGCTC 1 cut(s) 2398
EcoNI CCTNNNNNAGG 1 cut(s) 1508
EcoO109I RGGNCCY 1 cut(s) 1487
EcoRI GAATTC 3 cut(s) 809, 988, 1786
EcoT14I CCWWGG 2 cut(s) 1030, 1878
EcoT38I GRGCYC 2 cut(s) 1718, 2400
EgeI GGCGCC 1 cut(s) 369
EheI GGCGCC 1 cut(s) 369
ErhI CCWWGG 2 cut(s) 1030, 1878
FaeI CATG 7 cut(s) 104, 130, 383, 1736, 2299, 2515, 2726
FalI AAGNNNNNCTT 2 cut(s) 1983, 2015
FaqI GGGAC 3 cut(s) 1583, 1622, 2714
FatI CATG 7 cut(s) 100, 126, 379, 1732, 2295, 2511, 2722
FauI CCCGC 3 cut(s) 349, 355, 484
FauNDI CATATG 1 cut(s) 2558
FbaI TGATCA 1 cut(s) 1798
Fnu4HI GCNGC 5 cut(s) 267, 498, 636, 698, 1584
FokI GGATG 9 cut(s) 362, 381, 979, 986, 1652, 1879, 2149, 2447, 2528
FriOI GRGCYC 2 cut(s) 1718, 2400
Fsp4HI GCNGC 5 cut(s) 267, 498, 636, 698, 1584
FspAI RTGCGCAY 1 cut(s) 384
FspBI CTAG 8 cut(s) 651, 1031, 1272, 1817, 2057, 2180, 2333, 2375
FspI TGCGCA 1 cut(s) 384
GlaI GCGC 5 cut(s) 369, 384, 410, 1838, 1857
GluI GCNGC 5 cut(s) 267, 498, 636, 698, 1584
GsuI CTGGAG 1 cut(s) 1185
HaeII RGCGCY 2 cut(s) 371, 1859
HaeIII GGCC 4 cut(s) 105, 591, 2066, 2385
HapII CCGG 9 cut(s) 177, 203, 365, 476, 918, 1589, 1902, 2134, 2382
HhaI GCGC 5 cut(s) 370, 385, 411, 1839, 1858
Hin1I GRCGYC 1 cut(s) 368
Hin1II CATG 7 cut(s) 104, 130, 383, 1736, 2299, 2515, 2726
Hin6I GCGC 5 cut(s) 368, 383, 409, 1837, 1856
HinP1I GCGC 5 cut(s) 368, 383, 409, 1837, 1856
HincII GTYRAC 1 cut(s) 1192
HindII GTYRAC 1 cut(s) 1192
HindIII AAGCTT 1 cut(s) 1287
HpaII CCGG 9 cut(s) 177, 203, 365, 476, 918, 1589, 1902, 2134, 2382
Hpy166II GTNNAC 3 cut(s) 859, 1192, 2032
Hpy8I GTNNAC 3 cut(s) 859, 1192, 2032
Hpy99I CGWCG 2 cut(s) 307, 818
HpyCH4III ACNGT 5 cut(s) 275, 314, 742, 1164, 2525
HpyCH4IV ACGT 2 cut(s) 749, 2012
HpyF10VI GCNNNNNNNGC 7 cut(s) 397, 406, 497, 703, 911, 1202, 1286
HpySE526I ACGT 2 cut(s) 749, 2012
Hsp92I GRCGYC 1 cut(s) 368
Hsp92II CATG 7 cut(s) 104, 130, 383, 1736, 2299, 2515, 2726
HspAI GCGC 5 cut(s) 368, 383, 409, 1837, 1856
KasI GGCGCC 1 cut(s) 367
Ksp22I TGATCA 1 cut(s) 1798
LguI GCTCTTC 2 cut(s) 1174, 2099
Lsp1109I GCAGC 5 cut(s) 253, 484, 622, 709, 1570
LweI GCATC 6 cut(s) 400, 409, 531, 808, 1289, 2301
MaeI CTAG 8 cut(s) 651, 1031, 1272, 1817, 2057, 2180, 2333, 2375
MaeII ACGT 2 cut(s) 749, 2012
MaeIII GTNAC 4 cut(s) 750, 920, 1034, 2613
MbiI CCGCTC 2 cut(s) 344, 350
MfeI CAATTG 1 cut(s) 1174
MhlI GDGCHC 5 cut(s) 519, 1169, 1298, 1718, 2400
Mly113I GGCGCC 1 cut(s) 368
MlyI GAGTC 2 cut(s) 340, 1031
MmeI TCCRAC 5 cut(s) 666, 1020, 2005, 2125, 2697
MroXI GAANNNNTTC 5 cut(s) 242, 283, 2274, 2552, 2640
MslI CAYNNNNRTG 4 cut(s) 378, 572, 1061, 2649
MspA1I CMGCKG 4 cut(s) 266, 1022, 1796, 1937
MspI CCGG 9 cut(s) 177, 203, 365, 476, 918, 1589, 1902, 2134, 2382
MspR9I CCNGG 1 cut(s) 178
MunI CAATTG 1 cut(s) 1174
Mva1269I GAATGC 4 cut(s) 92, 199, 385, 2171
MvnI CGCG 1 cut(s) 2408
MwoI GCNNNNNNNGC 7 cut(s) 397, 406, 497, 703, 911, 1202, 1286
NarI GGCGCC 1 cut(s) 368
NciI CCSGG 1 cut(s) 178
NdeI CATATG 1 cut(s) 2558
NlaIII CATG 7 cut(s) 104, 130, 383, 1736, 2299, 2515, 2726
NlaIV GGNNCC 5 cut(s) 147, 369, 2164, 2215, 2778
NmeAIII GCCGAG 2 cut(s) 166, 498
NmuCI GTSAC 4 cut(s) 750, 920, 1034, 2613
NsbI TGCGCA 1 cut(s) 384
NspI RCATGY 1 cut(s) 1736
NspV TTCGAA 2 cut(s) 986, 1460
OliI CACNNNNGTG 2 cut(s) 572, 1061
PaqCI CACCTGC 1 cut(s) 126
PceI AGGCCT 1 cut(s) 2066
PciSI GCTCTTC 2 cut(s) 1174, 2099
PcsI WCGNNNNNNNCGW 2 cut(s) 1289, 2549
PctI GAATGC 4 cut(s) 92, 199, 385, 2171
PdmI GAANNNNTTC 5 cut(s) 242, 283, 2274, 2552, 2640
PfeI GAWTC 9 cut(s) 22, 163, 674, 941, 962, 1142, 1655, 2275, 2671
PflFI GACNNNGTC 1 cut(s) 1574
PinAI ACCGGT 2 cut(s) 475, 917
PkrI GCNGC 5 cut(s) 268, 499, 637, 699, 1585
PleI GAGTC 2 cut(s) 339, 1031
PluTI GGCGCC 1 cut(s) 371
PpsI GAGTC 2 cut(s) 339, 1031
PpuMI RGGWCCY 1 cut(s) 1487
Psp124BI GAGCTC 1 cut(s) 2400
Psp1406I AACGTT 1 cut(s) 2012
Psp5II RGGWCCY 1 cut(s) 1487
PspN4I GGNNCC 5 cut(s) 147, 369, 2164, 2215, 2778
PspPI GGNCC 1 cut(s) 1487
PspPPI RGGWCCY 1 cut(s) 1487
PsyI GACNNNGTC 1 cut(s) 1574
PvuII CAGCTG 2 cut(s) 266, 1022
RsaI GTAC 2 cut(s) 474, 1968
RsaNI GTAC 2 cut(s) 473, 1967
RseI CAYNNNNRTG 4 cut(s) 378, 572, 1061, 2649
SacI GAGCTC 1 cut(s) 2400
SapI GCTCTTC 2 cut(s) 1174, 2099
SatI GCNGC 5 cut(s) 267, 498, 636, 698, 1584
Sau96I GGNCC 1 cut(s) 1487
SchI GAGTC 2 cut(s) 340, 1031
ScrFI CCNGG 1 cut(s) 178
SduI GDGCHC 5 cut(s) 519, 1169, 1298, 1718, 2400
SfaNI GCATC 6 cut(s) 400, 409, 531, 808, 1289, 2301
SfcI CTRYAG 3 cut(s) 310, 1754, 2353
SfoI GGCGCC 1 cut(s) 369
SfuI TTCGAA 2 cut(s) 986, 1460
SinI GGWCC 1 cut(s) 1487
SmiMI CAYNNNNRTG 4 cut(s) 378, 572, 1061, 2649
SmlI CTYRAG 4 cut(s) 426, 770, 1739, 2192
SmoI CTYRAG 4 cut(s) 426, 770, 1739, 2192
SpeI ACTAGT 2 cut(s) 650, 1816
SseBI AGGCCT 1 cut(s) 2066
SspDI GGCGCC 1 cut(s) 367
SspI AATATT 1 cut(s) 2639
SspMI CTAG 8 cut(s) 651, 1031, 1272, 1817, 2057, 2180, 2333, 2375
SstI GAGCTC 1 cut(s) 2400
StuI AGGCCT 1 cut(s) 2066
StyD4I CCNGG 1 cut(s) 176
StyI CCWWGG 2 cut(s) 1030, 1878
TaaI ACNGT 5 cut(s) 275, 314, 742, 1164, 2525
TaiI ACGT 2 cut(s) 752, 2015
TfiI GAWTC 9 cut(s) 22, 163, 674, 941, 962, 1142, 1655, 2275, 2671
TscAI CASTG 8 cut(s) 280, 319, 844, 888, 1527, 2058, 2455, 2530
TseFI GTSAC 4 cut(s) 750, 920, 1034, 2613
TseI GCWGC 5 cut(s) 266, 497, 635, 697, 1583
Tsp45I GTSAC 4 cut(s) 750, 920, 1034, 2613
TspGWI ACGGA 3 cut(s) 291, 612, 2278
TspRI CASTG 8 cut(s) 280, 319, 844, 888, 1527, 2058, 2455, 2530
Tth111I GACNNNGTC 1 cut(s) 1574
VpaK11BI GGWCC 1 cut(s) 1487
XagI CCTNNNNNAGG 1 cut(s) 1508
XapI RAATTY 3 cut(s) 809, 988, 1786
XceI RCATGY 1 cut(s) 1736
XcmI CCANNNNNNNNNTGG 1 cut(s) 2428
XmaJI CCTAGG 1 cut(s) 1030
XmnI GAANNNNTTC 5 cut(s) 242, 283, 2274, 2552, 2640
XspI CTAG 8 cut(s) 651, 1031, 1272, 1817, 2057, 2180, 2333, 2375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.