MD12G1215100.v1.1

Belongs to the formin-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0005682
Physical Location & Seq
Forward (+)
29365566 .. 29368685
3120 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1215100.v1.1.491

Sequence Viewer

Length: 2775 bp
ATGGGATACATTTTCTCTCAGATTGTCCTCCTCATGATTTTTGTCATCATATCTTTTCTCATATCATTACACAATGCAGAGGAGGACTACTTGAATGTTCATGGCCTACAACACCACCTGCATTTCATGGAGGACAATGCCGACGCTCCCCAAAACCTACAAATGCACAAAGGTTCCGGGGAAGATGAAGAGAATGCAGTTTCAATTGTCGAAAATTTCGGAGCTTTACTTGGTTTGAACAGCTTCCACAAGCGAACGCCATCGGTTGGCGACAGTAGAAACGTTTCTCCTTCCCCTTCTCCAATTCCCTACTGCGAGGAGGAAGCTCCTAGTCCAGCTCCTGCTCCTGCTCCGGCGCCTCACATCCACGCGGGTTCCCATCATCCGCATCGCTTGCATTCGATCACAAAGCCTCAAAACATTCACAGAGAAGATAGAGGGGGAATCACGAGGATACTTGTGGCGGTTCTTGCTTCCACAGGAGCTGCAGTTGTTATCTGTGTGTTCGGAATTATTTGGGGTTGCAGGAAGCACAGAAAGCATAAGAAGAAACCCACGAGAGTGATTTCAGTTTTCGGAAAAAAAGGAGGAGCTAAATCAGCAAGCAAGGTGAGGTTAAATCCTGCCACTAATACTTCTGATCTCATTTATCTTGAATCGTTAGGGTTGGATTTAGAGCAGCAACATCAGCAGATTTCTTGTTCCAAAGAAACTTGTGAAACTGTAACTTCTTCACGAAATCATACATTGCTTGAGAGGGAAGAAACAAATCAAGAAGTGATAAAATCGGAATTCAATGATGCTAGTAGTAGTTCTTCCACTAGGGAAATTACGTCTATTCATGAGGATATGATTGCAGAGTCAGTGAAATATGACTCTGATTGTGCTAATTCCGCAACCGGTGACAAAATTACTCTCGTTAATTCTTATTCTTCCGATGATGAATCTTTCCATTCTTTCGGAGATTCGAATTCATCAAATGTCCGCATTTCGAATGCTTCCGCTGGTAGCCTAAATGAGTCATCAGACATTCTCTCCAAAAATATGTCAAATATAGAACCAGATCAACCAACAACCCTAAACCAAAAATCAATTCCGGCTCTTGAATTGCAGTCTCAGAGAAATGGAGAACGCGAAATTCTGAATGCACCGCGATCTTCCAATTGCGAAAAGAATGTCACATTCCCTCCAGCACCACCTCCTCCACCCCCACCACCAATCAAACACTTTCCTCATTTCCATTCTTCTCCTAGTTCAGCTATAATTGCGTCTAAAGCTTCGTGCCCTTCTTCTACATTGCGAAATCTATCACCGCCAAGAAAATCAGATTCTTCTTCTAGATCAAACCAAACCCAAGAAGGCGGATTTTCTGCTTCGCCTCAAAACTCCTCTAGAGCTTCAGAAACTCCACCTCATGTTCCTCCACCACCCAGCCCACCTCCCTTTTTGAAAGCGAATAATGGCTTTTTAAAATGTCCACCTCCTCCACCCCTCCCTCAAGCTGCGCCATTGGGAAGAGATGGAACTCCGTTGCCTAAACTGAAGCCGTTACATTGGGACAAAGTCAGAGCTGCACCGGATAATTCCATGGTGTGGGACAAGCTGAGATCAAGCTCATTTGAGTTGGACGAGGAAATGATTGAATCACTTTTCGGATACAATCTACAAAGATCAATGAAGAATGATGAAGCAAAATGCAAGAGCCCTTCTCCAAGCAAGCATGTTCTTGAGCCAAAGCGACTACAGAACATAACCATACTCTCAAAAGCCCTGAATTCTACTGCTGAGCAAGTATGCGAAGCACTAGTACAAGGCAATGGATTGTGTTTGCAACAATTGGAAGCACTTGTAAGGATGGAACCCACCAAGGAAGAAGAGGCTAAACTCACCGGCTTCAAAGGAGACATCAATGAACTAGGGTCCGCGGAGAGGTTTGTGAAGGCCGTGCTTAATGTGCCTTTCGCCTTCCAACGAGTTGAAGCAATGCTTTACAGAGAAACATTTGAAGATGAGGTGGTTCATCTCAGGAACTCCTTTTCAGTGCTAGAGGAGGCCTGCAAGGAACTGCGATCAAGCAGGCTCTTCTTGAAGCTACTCGAAGCCGTGTTGAAAACAGGAAATCGAATGAATGTGGGAACCATCCGAGGAGGAGCTAAAGCATTCAAGTTAGACACACTGCTCAAGCTCTCCGACATAAAAGGAACCGACGGAAAAACCACCTTGCTACATTTTGTTGTGCAAGAAATTATTAGGACAGAAGGGATTCGAGTTTCGGATAGCATCGTGGGGAGGATCAGCCAGAAGAACAAAACCAAAACCGTAGAAGAAAAAGAAGAAGACTACAGAAGAATGGGGCTTGACCTAGTTTCCGGCCTCAGCACCGAGCTCTACAACGCGAAGAAAACAGCCACATTGGACTTGGATGTTCTTGCAAGCAGTGTATCAAATCTCTCAGATGGAATGGATAAAATCAAACATCTGGTGCACGAGGATTTGTGCATGGATGAGAAGAGTGGTAATTTGGTGGATTCGATGAAATCGTTCATATGTTATGCAGAGAAGGGTTTGAGAGAGTTGCAGGGTGATGAGAGTAGGGTTTTGTCACAGGTGAAGGAGATTACAGAGTATTTTCATGGAAATGTGAGCAAGGAAGAAGCTAATCCGCTTCGGATTTTCGTGATTGTTAGGGACTTTTTGGGAATGTTGGATCATGTTTGTAAAGAGCTTAGAAGCTCAAAAGCTCGCACTCCAAATCCTCTTGCTCCATTCAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0008092
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FH2

Protein Analysis

925

Amino Acids

101.84

Weight (kDa)

6.56

Isoelectric Point (pI)

54.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FH2 PF02181 509 - 902 2.4e-106 Formin Homology 2 Domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 126
Acc36I ACCTGC 1 cut(s) 126
AccB1I GGYRCC 1 cut(s) 355
AccB7I CCANNNNNTGG 2 cut(s) 266, 1593
AccII CGCG 5 cut(s) 371, 1134, 1153, 1925, 2396
AclI AACGTT 1 cut(s) 282
AclWI GGATC 2 cut(s) 2300, 2715
AcsI RAATTY 5 cut(s) 214, 791, 970, 1137, 1774
AcuI CTGAAG 2 cut(s) 1383, 1562
AcyI GRCGYC 1 cut(s) 356
AfaI GTAC 1 cut(s) 1809
AfiI CCNNNNNNNGG 3 cut(s) 266, 1593, 1929
AgeI ACCGGT 1 cut(s) 899
AhlI ACTAGT 1 cut(s) 1804
AjuI GAANNNNNNNTTGG 2 cut(s) 1077, 1109
AleI CACNNNNGTG 1 cut(s) 560
Alw21I GWGCWC 2 cut(s) 2388, 2487
Alw26I GTCTC 2 cut(s) 1119, 1896
Alw44I GTGCAC 1 cut(s) 2483
AlwI GGATC 2 cut(s) 2300, 2715
AlwNI CAGNNNCTG 2 cut(s) 341, 485
AoxI GGCC 4 cut(s) 103, 1941, 2052, 2371
ApaLI GTGCAC 1 cut(s) 2483
ApeKI GCWGC 4 cut(s) 485, 679, 1502, 1571
ApoI RAATTY 5 cut(s) 214, 791, 970, 1137, 1774
ArsI GACNNNNNNTTYG 2 cut(s) 1161, 1193
AsiGI ACCGGT 1 cut(s) 899
Asp700I GAANNNNTTC 4 cut(s) 242, 283, 2262, 2540
AspLEI GCGC 2 cut(s) 358, 1507
AspS9I GGNCC 1 cut(s) 1920
AsuC2I CCSGG 1 cut(s) 178
AsuHPI GGTGA 6 cut(s) 622, 914, 1302, 1879, 2594, 2620
AsuII TTCGAA 2 cut(s) 968, 992
AvaII GGWCC 1 cut(s) 1920
BaeGI GKGCMC 2 cut(s) 1286, 2487
BanI GGYRCC 1 cut(s) 355
BanII GRGCYC 2 cut(s) 1706, 2388
BarI GAAGNNNNNNTAC 4 cut(s) 799, 831, 1791, 1823
BauI CACGAG 3 cut(s) 448, 556, 2486
BbsI GAAGAC 1 cut(s) 2343
Bbv12I GWGCWC 2 cut(s) 2388, 2487
BbvCI CCTCAGC 1 cut(s) 2375
BbvI GCAGC 4 cut(s) 472, 691, 1489, 1558
BccI CCATC 6 cut(s) 268, 387, 1514, 1849, 2147, 2450
BceAI ACGGC 3 cut(s) 1531, 1928, 2087
BciVI GTATCC 2 cut(s) 447, 1649
BcnI CCSGG 1 cut(s) 178
BcoDI GTCTC 2 cut(s) 1119, 1896
BcuI ACTAGT 1 cut(s) 1804
BfmI CTRYAG 3 cut(s) 486, 1742, 2341
BfoI RGCGCY 1 cut(s) 359
BfuAI ACCTGC 1 cut(s) 126
BfuI GTATCC 2 cut(s) 447, 1649
BisI GCNGC 4 cut(s) 486, 680, 1503, 1572
BlpI GCTNAGC 1 cut(s) 1785
BlsI GCNGC 4 cut(s) 487, 681, 1504, 1573
Bme1390I CCNGG 1 cut(s) 178
Bme18I GGWCC 1 cut(s) 1920
BmgT120I GGNCC 1 cut(s) 1920
BmiI GGNNCC 7 cut(s) 175, 357, 376, 1860, 1921, 2137, 2203
BmrFI CCNGG 1 cut(s) 178
BmsI GCATC 3 cut(s) 397, 790, 2289
BpiI GAAGAC 1 cut(s) 2343
BplI GAGNNNNNCTC 2 cut(s) 1693, 1725
BpmI CTGGAG 1 cut(s) 1173
Bpu10I CCTNAGC 1 cut(s) 2375
Bpu1102I GCTNAGC 1 cut(s) 1785
Bpu14I TTCGAA 2 cut(s) 968, 992
BpuEI CTTGAG 4 cut(s) 773, 1482, 1748, 2165
BpuMI CCSGG 1 cut(s) 178
BsaHI GRCGYC 1 cut(s) 356
BsaJI CCNNGG 5 cut(s) 177, 1587, 1866, 1923, 2143
BsaWI WCCGGW 2 cut(s) 899, 1576
BsaXI ACNNNNNCTCC 6 cut(s) 474, 504, 1019, 1049, 1171, 1201
Bsc4I CCNNNNNNNGG 3 cut(s) 266, 1593, 1929
Bse118I RCCGGY 2 cut(s) 899, 1889
Bse3DI GCAATG 4 cut(s) 746, 1295, 1822, 1989
BseDI CCNNGG 5 cut(s) 177, 1587, 1866, 1923, 2143
BseGI GGATG 6 cut(s) 363, 382, 1860, 2139, 2428, 2509
BseLI CCNNNNNNNGG 3 cut(s) 266, 1593, 1929
BseMI GCAATG 4 cut(s) 746, 1295, 1822, 1989
BseMII CTCAG 7 cut(s) 32, 1130, 1595, 1776, 2038, 2389, 2466
BseSI GKGCMC 2 cut(s) 1286, 2487
BseXI GCAGC 4 cut(s) 472, 691, 1489, 1558
BseYI CCCAGC 1 cut(s) 1430
BsgI GTGCAG 1 cut(s) 1557
Bsh1236I CGCG 5 cut(s) 371, 1134, 1153, 1925, 2396
BshFI GGCC 4 cut(s) 105, 1943, 2054, 2373
BshNI GGYRCC 1 cut(s) 355
BshTI ACCGGT 1 cut(s) 899
BsiHKAI GWGCWC 2 cut(s) 2388, 2487
BsiSI CCGG 7 cut(s) 177, 353, 900, 1097, 1577, 1890, 2370
BslFI GGGAC 3 cut(s) 1571, 1610, 2702
BslI CCNNNNNNNGG 3 cut(s) 266, 1593, 1929
BsmAI GTCTC 2 cut(s) 1119, 1896
BsmFI GGGAC 3 cut(s) 1571, 1610, 2702
BsmI GAATGC 5 cut(s) 199, 397, 1000, 1150, 2159
BsnI GGCC 4 cut(s) 105, 1943, 2054, 2373
Bsp119I TTCGAA 2 cut(s) 968, 992
Bsp1286I GDGCHC 4 cut(s) 1286, 1706, 2388, 2487
Bsp1720I GCTNAGC 1 cut(s) 1785
Bsp19I CCATGG 1 cut(s) 1587
BspANI GGCC 4 cut(s) 105, 1943, 2054, 2373
BspCNI CTCAG 7 cut(s) 31, 1129, 1596, 1777, 2037, 2388, 2465
BspFNI CGCG 5 cut(s) 371, 1134, 1153, 1925, 2396
BspHI TCATGA 2 cut(s) 33, 841
BspLI GGNNCC 7 cut(s) 175, 357, 376, 1860, 1921, 2137, 2203
BspMAI CTGCAG 1 cut(s) 490
BspMI ACCTGC 1 cut(s) 126
BspPI GGATC 2 cut(s) 2300, 2715
BspQI GCTCTTC 1 cut(s) 2087
BspT104I TTCGAA 2 cut(s) 968, 992
BspT107I GGYRCC 1 cut(s) 355
BsrDI GCAATG 4 cut(s) 746, 1295, 1822, 1989
BsrFI RCCGGY 2 cut(s) 899, 1889
BssAI RCCGGY 2 cut(s) 899, 1889
BssECI CCNNGG 5 cut(s) 177, 1587, 1866, 1923, 2143
BssNI GRCGYC 1 cut(s) 356
BssSI CACGAG 3 cut(s) 448, 556, 2486
BssT1I CCWWGG 2 cut(s) 1587, 1866
Bst2BI CACGAG 3 cut(s) 448, 556, 2486
Bst4CI ACNGT 3 cut(s) 275, 724, 2320
Bst6I CTCTTC 5 cut(s) 183, 1510, 1869, 2087, 2504
BstACI GRCGYC 1 cut(s) 356
BstAPI GCANNNNNTGC 1 cut(s) 394
BstBI TTCGAA 2 cut(s) 968, 992
BstC8I GCNNGC 7 cut(s) 395, 604, 1718, 2056, 2078, 2434, 2743
BstDEI CTNAG 8 cut(s) 18, 1116, 1604, 1785, 2024, 2375, 2452, 2726
BstDSI CCRYGG 2 cut(s) 1587, 1923
BstF5I GGATG 6 cut(s) 363, 382, 1860, 2139, 2428, 2509
BstFNI CGCG 5 cut(s) 371, 1134, 1153, 1925, 2396
BstH2I RGCGCY 1 cut(s) 359
BstHHI GCGC 2 cut(s) 358, 1507
BstMAI GTCTC 2 cut(s) 1119, 1896
BstMWI GCNNNNNNNGC 9 cut(s) 394, 470, 538, 599, 688, 893, 1265, 1274, 1954
BstNSI RCATGY 1 cut(s) 1724
BstSCI CCNGG 1 cut(s) 176
BstSFI CTRYAG 3 cut(s) 486, 1742, 2341
BstSLI GKGCMC 2 cut(s) 1286, 2487
BstUI CGCG 5 cut(s) 371, 1134, 1153, 1925, 2396
BstV1I GCAGC 4 cut(s) 472, 691, 1489, 1558
BstV2I GAAGAC 1 cut(s) 2343
BsuI GTATCC 2 cut(s) 447, 1649
BsuRI GGCC 4 cut(s) 105, 1943, 2054, 2373
BtgI CCRYGG 2 cut(s) 1587, 1923
BtgZI GCGATG 1 cut(s) 374
BtsCI GGATG 6 cut(s) 363, 382, 1860, 2139, 2428, 2509
BtsI GCAGTG 2 cut(s) 2174, 2443
BtsIMutI CAGTG 4 cut(s) 870, 2046, 2174, 2443
BveI ACCTGC 1 cut(s) 126
Cac8I GCNNGC 7 cut(s) 395, 604, 1718, 2056, 2078, 2434, 2743
CaiI CAGNNNCTG 2 cut(s) 341, 485
CciI TCATGA 2 cut(s) 33, 841
CfoI GCGC 2 cut(s) 358, 1507
Cfr10I RCCGGY 2 cut(s) 899, 1889
Cfr13I GGNCC 1 cut(s) 1920
Cfr42I CCGCGG 1 cut(s) 1926
CseI GACGC 2 cut(s) 152, 1257
Csp6I GTAC 1 cut(s) 1808
CspAI ACCGGT 1 cut(s) 899
CviQI GTAC 1 cut(s) 1808
DdeI CTNAG 8 cut(s) 18, 1116, 1604, 1785, 2024, 2375, 2452, 2726
DinI GGCGCC 1 cut(s) 357
DraI TTTAAA 1 cut(s) 1470
Eam1104I CTCTTC 5 cut(s) 183, 1510, 1869, 2087, 2504
EarI CTCTTC 5 cut(s) 183, 1510, 1869, 2087, 2504
EciI GGCGGA 1 cut(s) 1377
Ecl136II GAGCTC 1 cut(s) 2386
Eco130I CCWWGG 2 cut(s) 1587, 1866
Eco147I AGGCCT 1 cut(s) 2054
Eco24I GRGCYC 2 cut(s) 1706, 2388
Eco47I GGWCC 1 cut(s) 1920
Eco53kI GAGCTC 1 cut(s) 2386
Eco57I CTGAAG 2 cut(s) 1383, 1562
EcoICRI GAGCTC 1 cut(s) 2386
EcoRI GAATTC 3 cut(s) 791, 970, 1774
EcoT14I CCWWGG 2 cut(s) 1587, 1866
EcoT38I GRGCYC 2 cut(s) 1706, 2388
EgeI GGCGCC 1 cut(s) 357
EheI GGCGCC 1 cut(s) 357
ErhI CCWWGG 2 cut(s) 1587, 1866
FalI AAGNNNNNCTT 2 cut(s) 1971, 2003
FaqI GGGAC 3 cut(s) 1571, 1610, 2702
FauI CCCGC 1 cut(s) 364
FauNDI CATATG 1 cut(s) 2546
Fnu4HI GCNGC 4 cut(s) 486, 680, 1503, 1572
FokI GGATG 6 cut(s) 350, 369, 1867, 2126, 2435, 2516
FriOI GRGCYC 2 cut(s) 1706, 2388
Fsp4HI GCNGC 4 cut(s) 486, 680, 1503, 1572
GlaI GCGC 2 cut(s) 357, 1506
GluI GCNGC 4 cut(s) 486, 680, 1503, 1572
GsaI CCCAGC 1 cut(s) 1434
GsuI CTGGAG 1 cut(s) 1173
HaeII RGCGCY 1 cut(s) 359
HaeIII GGCC 4 cut(s) 105, 1943, 2054, 2373
HapII CCGG 7 cut(s) 177, 353, 900, 1097, 1577, 1890, 2370
HgaI GACGC 2 cut(s) 152, 1257
HhaI GCGC 2 cut(s) 358, 1507
Hin1I GRCGYC 1 cut(s) 356
Hin6I GCGC 2 cut(s) 356, 1505
HinP1I GCGC 2 cut(s) 356, 1505
HindIII AAGCTT 1 cut(s) 1275
HpaII CCGG 7 cut(s) 177, 353, 900, 1097, 1577, 1890, 2370
HphI GGTGA 6 cut(s) 622, 914, 1302, 1879, 2594, 2620
Hpy166II GTNNAC 2 cut(s) 1478, 2485
Hpy8I GTNNAC 2 cut(s) 1478, 2485
Hpy99I CGWCG 2 cut(s) 146, 2210
HpyCH4III ACNGT 3 cut(s) 275, 724, 2320
HpyCH4IV ACGT 2 cut(s) 282, 833
HpyF10VI GCNNNNNNNGC 9 cut(s) 394, 470, 538, 599, 688, 893, 1265, 1274, 1954
HpyF3I CTNAG 8 cut(s) 18, 1116, 1604, 1785, 2024, 2375, 2452, 2726
HpySE526I ACGT 2 cut(s) 282, 833
Hsp92I GRCGYC 1 cut(s) 356
HspAI GCGC 2 cut(s) 356, 1505
KasI GGCGCC 1 cut(s) 355
KspI CCGCGG 1 cut(s) 1926
LguI GCTCTTC 1 cut(s) 2087
Lsp1109I GCAGC 4 cut(s) 472, 691, 1489, 1558
LweI GCATC 3 cut(s) 397, 790, 2289
MaeII ACGT 2 cut(s) 282, 833
MaeIII GTNAC 5 cut(s) 724, 902, 1177, 1548, 2601
MfeI CAATTG 3 cut(s) 204, 1162, 1835
MhlI GDGCHC 4 cut(s) 1286, 1706, 2388, 2487
Mly113I GGCGCC 1 cut(s) 356
MlyI GAGTC 3 cut(s) 869, 869, 1028
MmeI TCCRAC 5 cut(s) 648, 1605, 1993, 2214, 2685
MroXI GAANNNNTTC 4 cut(s) 242, 283, 2262, 2540
MseI TTAA 4 cut(s) 617, 921, 1469, 1950
MslI CAYNNNNRTG 2 cut(s) 560, 2637
MspA1I CMGCKG 2 cut(s) 1004, 1925
MspI CCGG 7 cut(s) 177, 353, 900, 1097, 1577, 1890, 2370
MspR9I CCNGG 1 cut(s) 178
MunI CAATTG 3 cut(s) 204, 1162, 1835
Mva1269I GAATGC 5 cut(s) 199, 397, 1000, 1150, 2159
MvnI CGCG 5 cut(s) 371, 1134, 1153, 1925, 2396
MwoI GCNNNNNNNGC 9 cut(s) 394, 470, 538, 599, 688, 893, 1265, 1274, 1954
NarI GGCGCC 1 cut(s) 356
NciI CCSGG 1 cut(s) 178
NcoI CCATGG 1 cut(s) 1587
NdeI CATATG 1 cut(s) 2546
NlaIV GGNNCC 7 cut(s) 175, 357, 376, 1860, 1921, 2137, 2203
NmuCI GTSAC 3 cut(s) 902, 1177, 2601
NspI RCATGY 1 cut(s) 1724
NspV TTCGAA 2 cut(s) 968, 992
OliI CACNNNNGTG 1 cut(s) 560
PagI TCATGA 2 cut(s) 33, 841
PaqCI CACCTGC 1 cut(s) 126
PceI AGGCCT 1 cut(s) 2054
PciSI GCTCTTC 1 cut(s) 2087
PcsI WCGNNNNNNNCGW 1 cut(s) 2537
PctI GAATGC 5 cut(s) 199, 397, 1000, 1150, 2159
PdmI GAANNNNTTC 4 cut(s) 242, 283, 2262, 2540
PfeI GAWTC 8 cut(s) 444, 656, 944, 965, 1328, 1643, 2263, 2528
PflFI GACNNNGTC 1 cut(s) 1562
PflMI CCANNNNNTGG 2 cut(s) 266, 1593
PinAI ACCGGT 1 cut(s) 899
PkrI GCNGC 4 cut(s) 487, 681, 1504, 1573
PleI GAGTC 3 cut(s) 868, 869, 1027
PluTI GGCGCC 1 cut(s) 359
PpsI GAGTC 3 cut(s) 868, 869, 1027
Psp124BI GAGCTC 1 cut(s) 2388
Psp1406I AACGTT 1 cut(s) 282
PspFI CCCAGC 1 cut(s) 1430
PspN4I GGNNCC 7 cut(s) 175, 357, 376, 1860, 1921, 2137, 2203
PspPI GGNCC 1 cut(s) 1920
PstI CTGCAG 1 cut(s) 490
PstNI CAGNNNCTG 2 cut(s) 341, 485
PsyI GACNNNGTC 1 cut(s) 1562
RsaI GTAC 1 cut(s) 1809
RsaNI GTAC 1 cut(s) 1808
RseI CAYNNNNRTG 2 cut(s) 560, 2637
SacI GAGCTC 1 cut(s) 2388
SacII CCGCGG 1 cut(s) 1926
SapI GCTCTTC 1 cut(s) 2087
SaqAI TTAA 4 cut(s) 617, 921, 1469, 1950
SatI GCNGC 4 cut(s) 486, 680, 1503, 1572
Sau96I GGNCC 1 cut(s) 1920
SchI GAGTC 3 cut(s) 869, 869, 1028
ScrFI CCNGG 1 cut(s) 178
SduI GDGCHC 4 cut(s) 1286, 1706, 2388, 2487
SfaNI GCATC 3 cut(s) 397, 790, 2289
SfcI CTRYAG 3 cut(s) 486, 1742, 2341
SfoI GGCGCC 1 cut(s) 357
Sfr303I CCGCGG 1 cut(s) 1926
SfuI TTCGAA 2 cut(s) 968, 992
SgrBI CCGCGG 1 cut(s) 1926
SinI GGWCC 1 cut(s) 1920
SmiMI CAYNNNNRTG 2 cut(s) 560, 2637
SmlI CTYRAG 4 cut(s) 752, 1497, 1727, 2180
SmoI CTYRAG 4 cut(s) 752, 1497, 1727, 2180
SpeI ACTAGT 1 cut(s) 1804
SseBI AGGCCT 1 cut(s) 2054
SspDI GGCGCC 1 cut(s) 355
SstI GAGCTC 1 cut(s) 2388
StuI AGGCCT 1 cut(s) 2054
StyD4I CCNGG 1 cut(s) 176
StyI CCWWGG 2 cut(s) 1587, 1866
TaaI ACNGT 3 cut(s) 275, 724, 2320
TaiI ACGT 2 cut(s) 285, 836
TaqI TCGA 8 cut(s) 210, 401, 968, 992, 2097, 2122, 2266, 2531
TatI WGTACW 1 cut(s) 1807
TfiI GAWTC 8 cut(s) 444, 656, 944, 965, 1328, 1643, 2263, 2528
Tru1I TTAA 4 cut(s) 617, 921, 1469, 1950
Tru9I TTAA 4 cut(s) 617, 921, 1469, 1950
TscAI CASTG 4 cut(s) 870, 2046, 2181, 2443
TseFI GTSAC 3 cut(s) 902, 1177, 2601
TseI GCWGC 4 cut(s) 485, 679, 1502, 1571
Tsp45I GTSAC 3 cut(s) 902, 1177, 2601
TspGWI ACGGA 2 cut(s) 1518, 2223
TspRI CASTG 4 cut(s) 870, 2046, 2181, 2443
Tth111I GACNNNGTC 1 cut(s) 1562
Van91I CCANNNNNTGG 2 cut(s) 266, 1593
VneI GTGCAC 1 cut(s) 2483
VpaK11BI GGWCC 1 cut(s) 1920
XapI RAATTY 5 cut(s) 214, 791, 970, 1137, 1774
XbaI TCTAGA 2 cut(s) 1337, 1391
XceI RCATGY 1 cut(s) 1724
XcmI CCANNNNNNNNNTGG 1 cut(s) 2416
XmnI GAANNNNTTC 4 cut(s) 242, 283, 2262, 2540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.