Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0005682

Physical Location & Seq

Genomic Coordinates Forward (+)

45372854 .. 45379770

Gene Structure 6917 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00424180.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2670 bp

ATGGGTTATCATAGTTCTGTGATTTTCCACATGATGGAAATCTTCATCATGTTTCTTCTCTTTTCATTACTCAATACCACCCGCATTTTCACTGTTGGTGCTTTATCAGATGCAGAGGGCTTTAATGTTCATGGCCTACAGGAGCTGTATCATTCAGTGGAGGACAATGTTGAGGGCACTCAGAGCCTGCAGATTGAGAAGGTTTCTGGAGAAGATGAGAATGAAGAAAATAAGATTTCAGTGGTGGAAAAGTTCAGGGCCTTGCTTGGATTGAGGAGCTTACACAAGAGGACACCATCAAATGGTGACGGTGAAAATGTGTCTCCATCACCTTCTCCTTCTTCCCCTTCGGTGAAGCTGAGGCTCCAAGTCCGGCTCCTGCTCCTGCTCCTGCTCCGAGAGGATGATAAAGGCAGAACCAGAAAGATACTTATAGCTGTTCTTGTTTCAGCTGGAGCTGCTACTTTGATTAGTGTGCTTGGCCTCATATGGGGTTGCAGGAAGTACAAAGAGCACAAGAAGAAACCCACAAGAGTGATCTCACTTTACGGTGAAAAGGGAGGAGGAACAAGCGGTGGATCAAAGTATGTTGGTTCTCAAATACCAGCAAGCACGGTGAGCTTAAACCCTGGCACTCATGATCTTATTTATGTTGATTCATTAGGCATTGATGATGTAGAGCAACAACAACAACAGCCCTCTTGTCTTAAGCAAACTTGTGGAACTTTAAATGCCTCACCAAATCATTACACACAAAGATGCAAATTGAATGAGATGGAAGAGGAAGAATCTAATCAAGAAATGGTGAAACCAGATTTTGACAATGTTAGTAGTTCTTCCACTAGGGAAATTACCTCTGTTCATGAGGATAATGCAGACTCAGTCAAGTATGAGTCTGACTGTGCTAATTCTTCAGCTAGGGTTAAAGTTGTTCCCATTGATGCTTATTCTTCTGATGAGGAATCTTTCCATTCTTTTGGAGATTCCAATTCATCAAGCATCCGCCTTTCGAATGCTTCAGCAGGTAGTCTAGGCGACAACACAGCAGCAAATATGTCTACAAATGTGTCAAACAGAGAGGTCCACCTCCTCCACCCACTCCCTCCACTCACACCATTGGGAAAAGATGGAACACCGTTGCCAAAACTGAAGCCGCTACACTGGGACAAAGTTAGAGCTGCACCTGACCATTCTATGGTGTGGGACAAACTGAGATCAAGCTCATTTGAGTTTGATGAAGATATGATTGAATCTCTTTTCGGATACAATCTACAAAACACAATGAAGAATGATGAAGGAAAAAGCAAGACACCTTCTCCGAGCAAGCATGTGCTTGAGCCAAAGAGACTACAGAACATAACCATACTTTCAAAGGCCCTGAATGCTACTACAGAGCAAGTATGTGCAGCACTGGTACAAGGAAGTGGATTGTGTTTACAACAACTTGAAGCACTTGCGAAGATGGAGCCAACCAGTGAAGAAGTGGCTAAGCTTTCCGGCTATAAAGGAGATATTAAGGAACTGGGGTCAGTTGAGAAGTTTGTTAAGGCAGTGCTTAACGTTCCTTTCGCCTTTCAGCGAGTTGAAGCCATGCTTTACAGAGAAACTTTTGAAGATGAGGTGGTTCATCTTAGGAACTCGTTTTCAGTCCTAGAGGAGGCCTGCAAGGAACTCAGATCAAGCAGGCTGTTCTTGAAGTTACTTGAAGCCGTGTTGAAAACAGGCAACCGGATGAATGTGGGAACTATCAGAGGAGGTGCTAGAGCATTCAAGCTAGACGCGCTTCTCAAACTTGCTGATGTGAAAGGAACTGATGGAAAGACTACCTTGTTGCACTTTGTGGTGCAAGAAATAATAAGGGCAGAAGGGATCAGAGTTTCGGATAGCATAATGGGTAGGATCAGCCAGAAAAACAAAATCAAGACAGTTGAAGAGAAGGAAGAAGATTACAGAAGAATGGGGCTAGACCTCGTCTCCGGTCTCAGCACTGAACTCTACAATGCAAAGAAGACAGCTACATTGGATTTGGATGTTCTTGCAAGTGGTGTGTCAAACCTATCTGATGGAATGACCAAACTGAAGCATTTGATAAATGAAGAATTGGCCATGGATGAGAAAAGTGGGAACTTGGTGAGTTCCATGAAGTCTTTCATAAGCTATGCAGAGAAGAGTTTAAAAGAGTTGCAGGGTGATGAAAATAGAGTGCTGTCACATGTGAAGGAGATTACCGAGTACTTTCATGGAAATGTGAGCAAAGAAGAAGCAAACCCACTTAGAATTTTTGTGATTGTTAGAGACTTTTTGGGAATGTTTAATGATCCAGAGGTTCAGAGAGACATTAAGTTTTTGCCTTATAAGGTTGTGAATAGGGACGGGAAGCCTTATATTCAGGTCAAGGTTAAAGGTGAGGACAAGGTGTTTAGCCCTGAGGAAATCAGTGCTATGATACTGAGTAAGATGAAGGAAACAGCCGAGGCTTATTTGGGGAAGAAGATCACAAGGGCTGTCGTAACTGTTCCAGGTGAAAGAAGCTTAACAAAGGATTGTCATGAGCTCGGAAAATTTGATCTAACTGGCATTCCACCTGCTCCAAGGTACGTCTATGAGAAGAGTGGTGGAAGTTCTGCTGATTCGCAGGAGGACGAAGATGAACCAAATGATGAGTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0008092

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FH2

Protein Analysis

889

Amino Acids

98.47

Weight (kDa)

5.74

Isoelectric Point (pI)

40.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FH2	PF02181	376 - 772	4.7e-108	Formin Homology 2 Domain
HSP70	PF00012	751 - 840	1.3e-22	Hsp70 protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0005682)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05470
fragaria_vesca	FvH4_6g06480
malus_domestica	MD04G1201400.v1.1 MD12G1215100.v1.1
prunus_persica	Prupe.6G321600_v2.0.a1
pyrus_communis	pycom04g17740 pycom04g17750 pycom15g25860
rosa_chinensis	RchiOBHm_Chr3g0455601
rosa_laevigata	RLG00000025349
rosa_multiflora	Rmu_co8344379.1_g000001 Rmu_co8353267.1_g000001 Rmu_sc0000220.1_g000003 Rmu_sc0000223.1_g000001 Rmu_sc0005164.1_g000004 Rmu_sc0021585.1_g000001
rosa_roxburghii	Rroxscaffold_6G00423690 Rroxscaffold_6G00424180
rosa_rugosa	Rorug03G0008600 Rorug03G0008600
rosa_samantha	Rh2AG197000 Rh2DG204900 Rh3AG067900 Rh3BG070500 Rh3CG068800 Rh3DG070400 Rh7DG489800
rosa_wichuraiana	Rw3G005450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2355
AarI	CACCTGC	1 cut(s)	2593
Acc36I	ACCTGC	2 cut(s)	1013, 2593
AccB7I	CCANNNNNTGG	3 cut(s)	34, 302, 1195
AccI	GTMKAC	1 cut(s)	1058
AccII	CGCG	1 cut(s)	1781
AciI	CCGC	4 cut(s)	82, 573, 1003, 1154
AclI	AACGTT	1 cut(s)	1560
AclWI	GGATC	4 cut(s)	586, 1877, 1907, 2312
AcoI	YGGCCR	1 cut(s)	2103
AcsI	RAATTY	2 cut(s)	2277, 2561
AcuI	CTGAAG	4 cut(s)	897, 1002, 1169, 2099
AdeI	CACNNNGTG	1 cut(s)	1840
AfaI	GTAC	4 cut(s)	506, 1416, 2234, 2597
AfiI	CCNNNNNNNGG	5 cut(s)	34, 302, 490, 1195, 1657
AflII	CTTAAG	1 cut(s)	707
AflIII	ACRYGT	1 cut(s)	2212
AjnI	CCWGG	2 cut(s)	628, 2518
AleI	CACNNNNGTG	1 cut(s)	533
Alw21I	GWGCWC	2 cut(s)	516, 2556
Alw26I	GTCTC	6 cut(s)	327, 1339, 1978, 1985, 2289, 2328
AlwI	GGATC	4 cut(s)	586, 1877, 1907, 2312
AlwNI	CAGNNNCTG	2 cut(s)	145, 187
AoxI	GGCC	6 cut(s)	133, 258, 481, 1374, 1659, 2103
ApeKI	GCWGC	4 cut(s)	458, 1046, 1178, 1406
ApoI	RAATTY	2 cut(s)	2277, 2561
Asp700I	GAANNNNTTC	1 cut(s)	2147
AspLEI	GCGC	1 cut(s)	1783
AspS9I	GGNCC	3 cut(s)	258, 1081, 1375
AsuII	TTCGAA	1 cut(s)	1010
AvaII	GGWCC	1 cut(s)	1081
AxyI	CCTNAGG	1 cut(s)	2427
BaeGI	GKGCMC	1 cut(s)	179
BalI	TGGCCA	1 cut(s)	2105
BanII	GRGCYC	1 cut(s)	2556
BbsI	GAAGAC	1 cut(s)	2015
Bbv12I	GWGCWC	2 cut(s)	516, 2556
BbvCI	CCTCAGC	1 cut(s)	359
BbvI	GCAGC	4 cut(s)	445, 1058, 1165, 1418
BccI	CCATC	8 cut(s)	28, 304, 334, 769, 1121, 1456, 1808, 2057
BceAI	ACGGC	1 cut(s)	1694
BciT130I	CCWGG	2 cut(s)	630, 2520
BciVI	GTATCC	1 cut(s)	1256
BcoDI	GTCTC	6 cut(s)	327, 1339, 1978, 1985, 2289, 2328
BfaI	CTAG	7 cut(s)	843, 918, 1031, 1652, 1761, 1775, 1964
BfmI	CTRYAG	4 cut(s)	137, 188, 1349, 1389
BfrI	CTTAAG	1 cut(s)	707
BfuAI	ACCTGC	2 cut(s)	1013, 2593
BfuI	GTATCC	1 cut(s)	1256
BisI	GCNGC	5 cut(s)	459, 1047, 1154, 1179, 1407
BlpI	GCTNAGC	1 cut(s)	1488
BlsI	GCNGC	5 cut(s)	460, 1048, 1155, 1180, 1408
BmcAI	AGTACT	1 cut(s)	2234
Bme1390I	CCNGG	2 cut(s)	630, 2520
Bme18I	GGWCC	1 cut(s)	1081
BmgT120I	GGNCC	3 cut(s)	258, 1081, 1375
BmiI	GGNNCC	3 cut(s)	365, 377, 1467
BmrFI	CCNGG	2 cut(s)	630, 2520
BmrI	ACTGGG	2 cut(s)	1171, 1532
BmsI	GCATC	4 cut(s)	100, 749, 931, 1008
BmuI	ACTGGG	2 cut(s)	1171, 1532
BpiI	GAAGAC	1 cut(s)	2015
BpmI	CTGGAG	2 cut(s)	228, 474
Bpu10I	CCTNAGC	1 cut(s)	359
Bpu1102I	GCTNAGC	1 cut(s)	1488
Bpu14I	TTCGAA	1 cut(s)	1010
BpuEI	CTTGAG	1 cut(s)	1355
BsaBI	GATNNNNATC	1 cut(s)	38
BsaI	GGTCTC	1 cut(s)	1985
BsaJI	CCNNGG	4 cut(s)	628, 2106, 2472, 2591
BsaWI	WCCGGW	2 cut(s)	1728, 1976
BsaXI	ACNNNNNCTCC	4 cut(s)	1300, 1330, 1958, 1988
Bsc4I	CCNNNNNNNGG	5 cut(s)	34, 302, 490, 1195, 1657
Bse1I	ACTGG	5 cut(s)	1166, 1416, 1473, 1527, 2578
Bse21I	CCTNAGG	1 cut(s)	2427
Bse8I	GATNNNNATC	1 cut(s)	38
BseBI	CCWGG	2 cut(s)	630, 2520
BseDI	CCNNGG	4 cut(s)	628, 2106, 2472, 2591
BseGI	GGATG	5 cut(s)	409, 999, 1737, 2035, 2116
BseJI	GATNNNNATC	1 cut(s)	38
BseLI	CCNNNNNNNGG	5 cut(s)	34, 302, 490, 1195, 1657
BseMII	CTCAG	8 cut(s)	194, 350, 894, 1202, 1687, 1996, 2418, 2441
BseNI	ACTGG	5 cut(s)	1166, 1416, 1473, 1527, 2578
BseRI	GAGGAG	5 cut(s)	289, 576, 1079, 1670, 1767
BseSI	GKGCMC	1 cut(s)	179
BseXI	GCAGC	4 cut(s)	445, 1058, 1165, 1418
BsgI	GTGCAG	2 cut(s)	1164, 1425
Bsh1236I	CGCG	1 cut(s)	1781
BshFI	GGCC	6 cut(s)	135, 260, 483, 1376, 1661, 2105
BsiHKAI	GWGCWC	2 cut(s)	516, 2556
BsiSI	CCGG	4 cut(s)	373, 1497, 1729, 1977
BslFI	GGGAC	3 cut(s)	1178, 1217, 2384
BslI	CCNNNNNNNGG	5 cut(s)	34, 302, 490, 1195, 1657
BsmAI	GTCTC	6 cut(s)	327, 1339, 1978, 1985, 2289, 2328
BsmBI	CGTCTC	1 cut(s)	1978
BsmFI	GGGAC	3 cut(s)	1178, 1217, 2384
BsmI	GAATGC	4 cut(s)	1018, 1387, 1766, 2577
BsnI	GGCC	6 cut(s)	135, 260, 483, 1376, 1661, 2105
Bso31I	GGTCTC	1 cut(s)	1985
Bsp119I	TTCGAA	1 cut(s)	1010
Bsp1286I	GDGCHC	3 cut(s)	179, 516, 2556
Bsp1720I	GCTNAGC	1 cut(s)	1488
Bsp19I	CCATGG	1 cut(s)	2106
BspACI	CCGC	4 cut(s)	82, 573, 1003, 1154
BspANI	GGCC	6 cut(s)	135, 260, 483, 1376, 1661, 2105
BspCNI	CTCAG	8 cut(s)	193, 351, 893, 1203, 1686, 1995, 2419, 2442
BspFNI	CGCG	1 cut(s)	1781
BspHI	TCATGA	3 cut(s)	637, 862, 2548
BspLI	GGNNCC	3 cut(s)	365, 377, 1467
BspMAI	CTGCAG	1 cut(s)	192
BspMI	ACCTGC	2 cut(s)	1013, 2593
BspPI	GGATC	4 cut(s)	586, 1877, 1907, 2312
BspT104I	TTCGAA	1 cut(s)	1010
BspTI	CTTAAG	1 cut(s)	707
BspTNI	GGTCTC	1 cut(s)	1985
BsrI	ACTGG	5 cut(s)	1166, 1416, 1473, 1527, 2578
BssECI	CCNNGG	4 cut(s)	628, 2106, 2472, 2591
BssT1I	CCWWGG	2 cut(s)	2106, 2591
Bst2UI	CCWGG	2 cut(s)	630, 2520
Bst4CI	ACNGT	8 cut(s)	94, 311, 551, 616, 902, 1137, 1927, 2515
Bst6I	CTCTTC	4 cut(s)	774, 1926, 2162, 2603
BstAFI	CTTAAG	1 cut(s)	707
BstBI	TTCGAA	1 cut(s)	1010
BstC8I	GCNNGC	5 cut(s)	188, 610, 1325, 1663, 1685
BstDSI	CCRYGG	1 cut(s)	2106
BstENI	CCTNNNNNAGG	1 cut(s)	1655
BstF5I	GGATG	5 cut(s)	409, 999, 1737, 2035, 2116
BstFNI	CGCG	1 cut(s)	1781
BstHHI	GCGC	1 cut(s)	1783
BstMAI	GTCTC	6 cut(s)	327, 1339, 1978, 1985, 2289, 2328
BstMWI	GCNNNNNNNGC	5 cut(s)	183, 458, 618, 1382, 1780
BstNI	CCWGG	2 cut(s)	630, 2520
BstNSI	RCATGY	2 cut(s)	1331, 2216
BstSCI	CCNGG	2 cut(s)	628, 2518
BstSFI	CTRYAG	4 cut(s)	137, 188, 1349, 1389
BstSLI	GKGCMC	1 cut(s)	179
BstUI	CGCG	1 cut(s)	1781
BstV1I	GCAGC	4 cut(s)	445, 1058, 1165, 1418
BstV2I	GAAGAC	1 cut(s)	2015
BstXI	CCANNNNNNTGG	1 cut(s)	977
Bsu36I	CCTNAGG	1 cut(s)	2427
BsuI	GTATCC	1 cut(s)	1256
BsuRI	GGCC	6 cut(s)	135, 260, 483, 1376, 1661, 2105
BtgI	CCRYGG	1 cut(s)	2106
BtsCI	GGATG	5 cut(s)	409, 999, 1737, 2035, 2116
BtsI	GCAGTG	1 cut(s)	1557
BtsIMutI	CAGTG	9 cut(s)	90, 162, 246, 1159, 1409, 1480, 1557, 1986, 2443
BveI	ACCTGC	2 cut(s)	1013, 2593
Cac8I	GCNNGC	5 cut(s)	188, 610, 1325, 1663, 1685
CaiI	CAGNNNCTG	2 cut(s)	145, 187
CciI	TCATGA	3 cut(s)	637, 862, 2548
CfoI	GCGC	1 cut(s)	1783
Cfr13I	GGNCC	3 cut(s)	258, 1081, 1375
CseI	GACGC	1 cut(s)	1787
Csp6I	GTAC	4 cut(s)	505, 1415, 2233, 2596
CviQI	GTAC	4 cut(s)	505, 1415, 2233, 2596
DraI	TTTAAA	2 cut(s)	729, 2175
DraIII	CACNNNGTG	1 cut(s)	1840
EaeI	YGGCCR	1 cut(s)	2103
Eam1104I	CTCTTC	4 cut(s)	774, 1926, 2162, 2603
EarI	CTCTTC	4 cut(s)	774, 1926, 2162, 2603
EciI	GGCGGA	1 cut(s)	992
Ecl136II	GAGCTC	1 cut(s)	2554
Eco130I	CCWWGG	2 cut(s)	2106, 2591
Eco147I	AGGCCT	1 cut(s)	1661
Eco24I	GRGCYC	1 cut(s)	2556
Eco31I	GGTCTC	1 cut(s)	1985
Eco47I	GGWCC	1 cut(s)	1081
Eco53kI	GAGCTC	1 cut(s)	2554
Eco57I	CTGAAG	4 cut(s)	897, 1002, 1169, 2099
Eco81I	CCTNAGG	1 cut(s)	2427
EcoICRI	GAGCTC	1 cut(s)	2554
EcoNI	CCTNNNNNAGG	1 cut(s)	1655
EcoO109I	RGGNCCY	2 cut(s)	258, 1375
EcoRII	CCWGG	2 cut(s)	628, 2518
EcoT14I	CCWWGG	2 cut(s)	2106, 2591
EcoT38I	GRGCYC	1 cut(s)	2556
ErhI	CCWWGG	2 cut(s)	2106, 2591
Esp3I	CGTCTC	1 cut(s)	1978
FalI	AAGNNNNNCTT	4 cut(s)	1578, 1610, 1811, 1843
FaqI	GGGAC	3 cut(s)	1178, 1217, 2384
FauI	CCCGC	1 cut(s)	89
FauNDI	CATATG	1 cut(s)	488
FblI	GTMKAC	1 cut(s)	1058
Fnu4HI	GCNGC	5 cut(s)	459, 1047, 1154, 1179, 1407
FokI	GGATG	5 cut(s)	416, 986, 1744, 2042, 2123
FriOI	GRGCYC	1 cut(s)	2556
Fsp4HI	GCNGC	5 cut(s)	459, 1047, 1154, 1179, 1407
FspBI	CTAG	7 cut(s)	843, 918, 1031, 1652, 1761, 1775, 1964
GlaI	GCGC	1 cut(s)	1782
GluI	GCNGC	5 cut(s)	459, 1047, 1154, 1179, 1407
GsuI	CTGGAG	2 cut(s)	228, 474
HaeIII	GGCC	6 cut(s)	135, 260, 483, 1376, 1661, 2105
HapII	CCGG	4 cut(s)	373, 1497, 1729, 1977
HgaI	GACGC	1 cut(s)	1787
HhaI	GCGC	1 cut(s)	1783
Hin6I	GCGC	1 cut(s)	1781
HinP1I	GCGC	1 cut(s)	1781
HindIII	AAGCTT	2 cut(s)	1490, 2530
HinfI	GANTC	8 cut(s)	656, 788, 878, 893, 962, 983, 1250, 2630
HpaII	CCGG	4 cut(s)	373, 1497, 1729, 1977
Hpy166II	GTNNAC	3 cut(s)	1059, 1084, 1436
Hpy188III	TCNNGA	8 cut(s)	207, 638, 797, 863, 1693, 1921, 2321, 2549
Hpy8I	GTNNAC	3 cut(s)	1059, 1084, 1436
HpyCH4III	ACNGT	8 cut(s)	94, 311, 551, 616, 902, 1137, 1927, 2515
HpyCH4IV	ACGT	2 cut(s)	1560, 2598
HpyF10VI	GCNNNNNNNGC	5 cut(s)	183, 458, 618, 1382, 1780
HpySE526I	ACGT	2 cut(s)	1560, 2598
HspAI	GCGC	1 cut(s)	1781
Lsp1109I	GCAGC	4 cut(s)	445, 1058, 1165, 1418
LweI	GCATC	4 cut(s)	100, 749, 931, 1008
MaeI	CTAG	7 cut(s)	843, 918, 1031, 1652, 1761, 1775, 1964
MaeII	ACGT	2 cut(s)	1560, 2598
MaeIII	GTNAC	4 cut(s)	305, 1698, 2208, 2509
MhlI	GDGCHC	3 cut(s)	179, 516, 2556
MlsI	TGGCCA	1 cut(s)	2105
MluCI	AATT	7 cut(s)	764, 849, 907, 988, 2099, 2277, 2561
MluNI	TGGCCA	1 cut(s)	2105
MlyI	GAGTC	2 cut(s)	872, 902
Mox20I	TGGCCA	1 cut(s)	2105
MroXI	GAANNNNTTC	1 cut(s)	2147
MscI	TGGCCA	1 cut(s)	2105
MslI	CAYNNNNRTG	3 cut(s)	533, 757, 2244
Msp20I	TGGCCA	1 cut(s)	2105
MspA1I	CMGCKG	1 cut(s)	452
MspCI	CTTAAG	1 cut(s)	707
MspI	CCGG	4 cut(s)	373, 1497, 1729, 1977
MspR9I	CCNGG	2 cut(s)	630, 2520
Mva1269I	GAATGC	4 cut(s)	1018, 1387, 1766, 2577
MvaI	CCWGG	2 cut(s)	630, 2520
MvnI	CGCG	1 cut(s)	1781
MwoI	GCNNNNNNNGC	5 cut(s)	183, 458, 618, 1382, 1780
NcoI	CCATGG	1 cut(s)	2106
NdeI	CATATG	1 cut(s)	488
NlaIV	GGNNCC	3 cut(s)	365, 377, 1467
NmeAIII	GCCGAG	1 cut(s)	2497
NmuCI	GTSAC	2 cut(s)	305, 2208
NspI	RCATGY	2 cut(s)	1331, 2216
NspV	TTCGAA	1 cut(s)	1010
OliI	CACNNNNGTG	1 cut(s)	533
PagI	TCATGA	3 cut(s)	637, 862, 2548
PaqCI	CACCTGC	1 cut(s)	2593
PceI	AGGCCT	1 cut(s)	1661
PciI	ACATGT	1 cut(s)	2212
PctI	GAATGC	4 cut(s)	1018, 1387, 1766, 2577
PdmI	GAANNNNTTC	1 cut(s)	2147
PfeI	GAWTC	6 cut(s)	656, 788, 962, 983, 1250, 2630
PflFI	GACNNNGTC	2 cut(s)	881, 1970
PflMI	CCANNNNNTGG	3 cut(s)	34, 302, 1195
PkrI	GCNGC	5 cut(s)	460, 1048, 1155, 1180, 1408
PleI	GAGTC	2 cut(s)	872, 901
PpsI	GAGTC	2 cut(s)	872, 901
PscI	ACATGT	1 cut(s)	2212
PsiI	TTATAA	1 cut(s)	2355
Psp124BI	GAGCTC	1 cut(s)	2556
Psp1406I	AACGTT	1 cut(s)	1560
Psp6I	CCWGG	2 cut(s)	628, 2518
PspGI	CCWGG	2 cut(s)	628, 2518
PspN4I	GGNNCC	3 cut(s)	365, 377, 1467
PspPI	GGNCC	3 cut(s)	258, 1081, 1375
PstI	CTGCAG	1 cut(s)	192
PstNI	CAGNNNCTG	2 cut(s)	145, 187
PsyI	GACNNNGTC	2 cut(s)	881, 1970
PvuII	CAGCTG	1 cut(s)	452
RsaI	GTAC	4 cut(s)	506, 1416, 2234, 2597
RsaNI	GTAC	4 cut(s)	505, 1415, 2233, 2596
RseI	CAYNNNNRTG	3 cut(s)	533, 757, 2244
SacI	GAGCTC	1 cut(s)	2556
SatI	GCNGC	5 cut(s)	459, 1047, 1154, 1179, 1407
Sau96I	GGNCC	3 cut(s)	258, 1081, 1375
ScaI	AGTACT	1 cut(s)	2234
SchI	GAGTC	2 cut(s)	872, 902
ScrFI	CCNGG	2 cut(s)	630, 2520
SduI	GDGCHC	3 cut(s)	179, 516, 2556
SfaNI	GCATC	4 cut(s)	100, 749, 931, 1008
SfcI	CTRYAG	4 cut(s)	137, 188, 1349, 1389
SfuI	TTCGAA	1 cut(s)	1010
SinI	GGWCC	1 cut(s)	1081
SmiMI	CAYNNNNRTG	3 cut(s)	533, 757, 2244
SmlI	CTYRAG	2 cut(s)	707, 1334
SmoI	CTYRAG	2 cut(s)	707, 1334
Sse9I	AATT	7 cut(s)	764, 849, 907, 988, 2099, 2277, 2561
SseBI	AGGCCT	1 cut(s)	1661
SsiI	CCGC	4 cut(s)	82, 573, 1003, 1154
SspMI	CTAG	7 cut(s)	843, 918, 1031, 1652, 1761, 1775, 1964
SstI	GAGCTC	1 cut(s)	2556
StuI	AGGCCT	1 cut(s)	1661
StyD4I	CCNGG	2 cut(s)	628, 2518
StyI	CCWWGG	2 cut(s)	2106, 2591
TaaI	ACNGT	8 cut(s)	94, 311, 551, 616, 902, 1137, 1927, 2515
TaiI	ACGT	2 cut(s)	1563, 2601
TaqI	TCGA	1 cut(s)	1010
TasI	AATT	7 cut(s)	764, 849, 907, 988, 2099, 2277, 2561
TatI	WGTACW	2 cut(s)	504, 2232
TauI	GCSGC	1 cut(s)	1156
TfiI	GAWTC	6 cut(s)	656, 788, 962, 983, 1250, 2630
TscAI	CASTG	9 cut(s)	97, 162, 246, 1166, 1416, 1480, 1557, 1993, 2443
TseFI	GTSAC	2 cut(s)	305, 2208
TseI	GCWGC	4 cut(s)	458, 1046, 1178, 1406
Tsp45I	GTSAC	2 cut(s)	305, 2208
TspRI	CASTG	9 cut(s)	97, 162, 246, 1166, 1416, 1480, 1557, 1993, 2443
Tth111I	GACNNNGTC	2 cut(s)	881, 1970
Van91I	CCANNNNNTGG	3 cut(s)	34, 302, 1195
Vha464I	CTTAAG	1 cut(s)	707
VpaK11BI	GGWCC	1 cut(s)	1081
XagI	CCTNNNNNAGG	1 cut(s)	1655
XapI	RAATTY	2 cut(s)	2277, 2561
XceI	RCATGY	2 cut(s)	1331, 2216
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1480
XmiI	GTMKAC	1 cut(s)	1058
XmnI	GAANNNNTTC	1 cut(s)	2147
XspI	CTAG	7 cut(s)	843, 918, 1031, 1652, 1761, 1775, 1964
ZrmI	AGTACT	1 cut(s)	2234

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.