Rh2DG204900

Belongs to the formin-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0005682
Physical Location & Seq
Forward (+)
19074665 .. 19075735
1071 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG204900.1

Sequence Viewer

Length: 969 bp
ATGGCACCAACAAAGGAAGAAGAAATTAAACTTCGAGAGTACCAGGGTGACATCTCAAAATTAGGGTCTGCTGAACGGTTTCTAAAGGCAGTGCTTGATATCCCATTTGCTTTTAAGAGAGTTGAGGCAATGCTATACAGAGCTAACTTTGATACAGAAGTGAAATACCTAAGGAAGTCTTTTCAAACGCTCGAGGAAGCAAGTGAAGAATTGAAAAACAGTCGATTATTCCTAAAACTCCTTGAAGCTGTTCTCAGGACTGGAAACCGAATGAATGTTGGGACCAACCGTGGTGATGCTAAAGCTTTCAAACTTGATACGCTCTTAAAACTGGTCGACATAAAGGGAACAGATGGAAAGACCACATTGCTCCACTTTGTGGTTCAAGAGATTATAAGATCTGAAAGTCCAGGTTCTGAAACAAAGGAGAACATCAAGACAAAGGAAGGTGATTTCAAAAAGCAAGGATTGAAAATGGTGGCAGGGCTGAGCAAAGACCTCGGCAACGTCGCAAAAGCAGCAGGAATGGACTCAGACGTCCTAAGTAGCTATGTGTCAAAGCTTGCAATGGGGCTTGAGAAGGTCAGACTGGTCTTGCAGTATGACAAGGCAGATGTAGATGGAAAATTCTTCAACTCGATGAAGCTCTTTCTAAAAGAGGCAGAGGAGGAAATTCTTAGGATCAAGGCTGATGAAAGAAAGGCTTTATTTCTGGTGAAAGAGGTTACTCAATATTTCCATGGGGATACAGCAAAGGAGGAAGCCCATCCTTTCAGAATCTTCATGATCGTAAGAGATTTTCTAAAAGTATTGGACCATGTGTGCAAGGAAGTCGGGAATATGCAGGACAGAACACTGGTGGGTTCTGCTAGGTCATTCCGGATAGCTGCAACTGCTCCTCTACCAGTTCTCTCCAAGTATCACTTAAGACAGGAAGACACCTCGGACGACGACAGCTTTTCTCTTTGA

Protein Analysis

322

Amino Acids

36.49

Weight (kDa)

8.63

Isoelectric Point (pI)

26.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FH2 PF02181 1 - 271 7.6e-80 Formin Homology 2 Domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 395
AasI GACNNNNNNGTC 1 cut(s) 536
AatII GACGTC 1 cut(s) 540
AccB1I GGYRCC 1 cut(s) 4
AccB7I CCANNNNNTGG 1 cut(s) 379
AccI GTMKAC 1 cut(s) 336
AccIII TCCGGA 1 cut(s) 879
AclWI GGATC 1 cut(s) 689
AcsI RAATTY 2 cut(s) 626, 672
AcyI GRCGYC 1 cut(s) 537
AdeI CACNNNGTG 1 cut(s) 379
AfaI GTAC 1 cut(s) 41
AfiI CCNNNNNNNGG 1 cut(s) 379
AflII CTTAAG 1 cut(s) 925
AgsI TTSAA 8 cut(s) 185, 214, 245, 310, 386, 457, 472, 634
AjnI CCWGG 2 cut(s) 42, 409
AluBI AGCT 8 cut(s) 143, 248, 305, 549, 562, 646, 887, 957
AluI AGCT 8 cut(s) 143, 248, 305, 549, 562, 646, 887, 957
AlwI GGATC 1 cut(s) 689
AlwNI CAGNNNCTG 1 cut(s) 416
Ama87I CYCGRG 1 cut(s) 191
Aor13HI TCCGGA 1 cut(s) 879
ApeKI GCWGC 2 cut(s) 518, 887
ApoI RAATTY 2 cut(s) 626, 672
ArsI GACNNNNNNTTYG 2 cut(s) 50, 82
Asp700I GAANNNNTTC 2 cut(s) 78, 249
AspS9I GGNCC 2 cut(s) 282, 814
AsuHPI GGTGA 4 cut(s) 59, 305, 461, 727
AvaI CYCGRG 1 cut(s) 191
AvaII GGWCC 2 cut(s) 282, 814
AxyI CCTNAGG 1 cut(s) 170
BaeI ACNNNNGTAYC 2 cut(s) 144, 177
BanI GGYRCC 1 cut(s) 4
BbsI GAAGAC 1 cut(s) 942
BbvI GCAGC 2 cut(s) 530, 874
BccI CCATC 3 cut(s) 347, 614, 774
BcgI CGANNNNNNTGC 4 cut(s) 481, 515, 814, 848
BciT130I CCWGG 2 cut(s) 44, 411
BciVI GTATCC 1 cut(s) 739
BfaI CTAG 1 cut(s) 870
BfrI CTTAAG 1 cut(s) 925
BfuI GTATCC 1 cut(s) 739
BglII AGATCT 1 cut(s) 398
BisI GCNGC 2 cut(s) 519, 888
BlpI GCTNAGC 1 cut(s) 488
BlsI GCNGC 2 cut(s) 520, 889
Bme1390I CCNGG 2 cut(s) 44, 411
Bme18I GGWCC 2 cut(s) 282, 814
BmeT110I CYCGRG 1 cut(s) 191
BmgT120I GGNCC 2 cut(s) 282, 814
BmiI GGNNCC 2 cut(s) 6, 283
BmrFI CCNGG 2 cut(s) 44, 411
BmsI GCATC 1 cut(s) 286
BpiI GAAGAC 1 cut(s) 942
Bpu1102I GCTNAGC 1 cut(s) 488
BpuEI CTTGAG 1 cut(s) 596
BsaHI GRCGYC 1 cut(s) 537
BsaJI CCNNGG 5 cut(s) 43, 289, 499, 739, 942
BsaWI WCCGGW 1 cut(s) 879
Bsc4I CCNNNNNNNGG 1 cut(s) 379
Bse1I ACTGG 5 cut(s) 265, 336, 594, 861, 905
Bse21I CCTNAGG 1 cut(s) 170
Bse3DI GCAATG 3 cut(s) 135, 365, 573
BseAI TCCGGA 1 cut(s) 879
BseBI CCWGG 2 cut(s) 44, 411
BseDI CCNNGG 5 cut(s) 43, 289, 499, 739, 942
BseGI GGATG 1 cut(s) 766
BseLI CCNNNNNNNGG 1 cut(s) 379
BseMI GCAATG 3 cut(s) 135, 365, 573
BseMII CTCAG 3 cut(s) 268, 479, 546
BseNI ACTGG 5 cut(s) 265, 336, 594, 861, 905
BseRI GAGGAG 2 cut(s) 680, 888
BseXI GCAGC 2 cut(s) 530, 874
BshNI GGYRCC 1 cut(s) 4
BsiHKCI CYCGRG 1 cut(s) 191
BsiSI CCGG 1 cut(s) 880
BslFI GGGAC 1 cut(s) 295
BslI CCNNNNNNNGG 1 cut(s) 379
BsmFI GGGAC 1 cut(s) 295
BsoBI CYCGRG 1 cut(s) 191
Bsp13I TCCGGA 1 cut(s) 879
Bsp143I GATC 3 cut(s) 398, 681, 786
Bsp1720I GCTNAGC 1 cut(s) 488
Bsp19I CCATGG 1 cut(s) 739
BspCNI CTCAG 3 cut(s) 267, 480, 545
BspEI TCCGGA 1 cut(s) 879
BspHI TCATGA 1 cut(s) 783
BspLI GGNNCC 2 cut(s) 6, 283
BspPI GGATC 1 cut(s) 689
BspT107I GGYRCC 1 cut(s) 4
BspTI CTTAAG 1 cut(s) 925
BsrDI GCAATG 3 cut(s) 135, 365, 573
BsrI ACTGG 5 cut(s) 265, 336, 594, 861, 905
BssECI CCNNGG 5 cut(s) 43, 289, 499, 739, 942
BssMI GATC 3 cut(s) 398, 681, 786
BssNI GRCGYC 1 cut(s) 537
BssT1I CCWWGG 1 cut(s) 739
Bst2UI CCWGG 2 cut(s) 44, 411
Bst4CI ACNGT 3 cut(s) 78, 221, 290
BstACI GRCGYC 1 cut(s) 537
BstAFI CTTAAG 1 cut(s) 925
BstC8I GCNNGC 1 cut(s) 564
BstDEI CTNAG 6 cut(s) 170, 254, 488, 532, 542, 677
BstDSI CCRYGG 2 cut(s) 289, 739
BstF5I GGATG 1 cut(s) 766
BstKTI GATC 3 cut(s) 401, 684, 789
BstMBI GATC 3 cut(s) 398, 681, 786
BstMWI GCNNNNNNNGC 2 cut(s) 518, 893
BstNI CCWGG 2 cut(s) 44, 411
BstSCI CCNGG 2 cut(s) 42, 409
BstV1I GCAGC 2 cut(s) 530, 874
BstV2I GAAGAC 1 cut(s) 942
BstX2I RGATCY 1 cut(s) 398
BstYI RGATCY 1 cut(s) 398
Bsu36I CCTNAGG 1 cut(s) 170
BsuI GTATCC 1 cut(s) 739
BtgI CCRYGG 2 cut(s) 289, 739
BtsCI GGATG 1 cut(s) 766
BtsI GCAGTG 1 cut(s) 96
BtsIMutI CAGTG 2 cut(s) 96, 854
Cac8I GCNNGC 1 cut(s) 564
CaiI CAGNNNCTG 1 cut(s) 416
CciI TCATGA 1 cut(s) 783
Cfr13I GGNCC 2 cut(s) 282, 814
Csp6I GTAC 1 cut(s) 40
CviAII CATG 3 cut(s) 740, 784, 818
CviQI GTAC 1 cut(s) 40
DdeI CTNAG 6 cut(s) 170, 254, 488, 532, 542, 677
DpnI GATC 3 cut(s) 400, 683, 788
DpnII GATC 3 cut(s) 398, 681, 786
DraIII CACNNNGTG 1 cut(s) 379
DrdI GACNNNNNNGTC 1 cut(s) 536
DseDI GACNNNNNNGTC 1 cut(s) 536
Eco130I CCWWGG 1 cut(s) 739
Eco32I GATATC 1 cut(s) 100
Eco47I GGWCC 2 cut(s) 282, 814
Eco81I CCTNAGG 1 cut(s) 170
Eco88I CYCGRG 1 cut(s) 191
EcoRII CCWGG 2 cut(s) 42, 409
EcoRV GATATC 1 cut(s) 100
EcoT14I CCWWGG 1 cut(s) 739
ErhI CCWWGG 1 cut(s) 739
FaeI CATG 3 cut(s) 743, 787, 821
FaiI YATR 9 cut(s) 136, 341, 395, 552, 603, 741, 785, 819, 842
FalI AAGNNNNNCTT 6 cut(s) 163, 195, 688, 720, 908, 940
FaqI GGGAC 1 cut(s) 295
FatI CATG 3 cut(s) 739, 783, 817
FblI GTMKAC 1 cut(s) 336
Fnu4HI GCNGC 2 cut(s) 519, 888
FokI GGATG 1 cut(s) 753
Fsp4HI GCNGC 2 cut(s) 519, 888
FspBI CTAG 1 cut(s) 870
GluI GCNGC 2 cut(s) 519, 888
HapII CCGG 1 cut(s) 880
Hin1I GRCGYC 1 cut(s) 537
Hin1II CATG 3 cut(s) 743, 787, 821
HincII GTYRAC 1 cut(s) 337
HindII GTYRAC 1 cut(s) 337
HindIII AAGCTT 2 cut(s) 303, 560
HinfI GANTC 2 cut(s) 530, 777
HpaII CCGG 1 cut(s) 880
HphI GGTGA 4 cut(s) 59, 305, 461, 727
Hpy166II GTNNAC 1 cut(s) 337
Hpy188I TCNGA 6 cut(s) 403, 418, 535, 587, 776, 946
Hpy188III TCNNGA 7 cut(s) 35, 256, 386, 436, 784, 835, 880
Hpy8I GTNNAC 1 cut(s) 337
Hpy99I CGWCG 2 cut(s) 512, 953
HpyAV CCTTC 2 cut(s) 440, 574
HpyCH4III ACNGT 3 cut(s) 78, 221, 290
HpyCH4IV ACGT 2 cut(s) 507, 537
HpyCH4V TGCA 5 cut(s) 566, 598, 825, 844, 890
HpyF10VI GCNNNNNNNGC 2 cut(s) 518, 893
HpyF3I CTNAG 6 cut(s) 170, 254, 488, 532, 542, 677
HpySE526I ACGT 2 cut(s) 507, 537
Hsp92I GRCGYC 1 cut(s) 537
Hsp92II CATG 3 cut(s) 743, 787, 821
Kpn2I TCCGGA 1 cut(s) 879
Kzo9I GATC 3 cut(s) 398, 681, 786
LmnI GCTCC 2 cut(s) 375, 901
Lsp1109I GCAGC 2 cut(s) 530, 874
LweI GCATC 1 cut(s) 286
MaeI CTAG 1 cut(s) 870
MaeII ACGT 2 cut(s) 507, 537
MaeIII GTNAC 2 cut(s) 47, 724
MalI GATC 3 cut(s) 400, 683, 788
MboI GATC 3 cut(s) 398, 681, 786
MboII GAAGA 6 cut(s) 29, 32, 218, 622, 772, 947
MflI RGATCY 1 cut(s) 398
MluCI AATT 5 cut(s) 24, 59, 209, 626, 672
MlyI GAGTC 1 cut(s) 524
MroI TCCGGA 1 cut(s) 879
MroXI GAANNNNTTC 2 cut(s) 78, 249
MseI TTAA 4 cut(s) 27, 114, 326, 926
MspCI CTTAAG 1 cut(s) 925
MspI CCGG 1 cut(s) 880
MspR9I CCNGG 2 cut(s) 44, 411
MvaI CCWGG 2 cut(s) 44, 411
MwoI GCNNNNNNNGC 2 cut(s) 518, 893
NcoI CCATGG 1 cut(s) 739
NdeII GATC 3 cut(s) 398, 681, 786
NlaIII CATG 3 cut(s) 743, 787, 821
NlaIV GGNNCC 2 cut(s) 6, 283
NmeAIII GCCGAG 1 cut(s) 480
NmuCI GTSAC 1 cut(s) 47
PaeR7I CTCGAG 1 cut(s) 191
PagI TCATGA 1 cut(s) 783
PdmI GAANNNNTTC 2 cut(s) 78, 249
PfeI GAWTC 1 cut(s) 777
PflMI CCANNNNNTGG 1 cut(s) 379
PkrI GCNGC 2 cut(s) 520, 889
PleI GAGTC 1 cut(s) 524
PpsI GAGTC 1 cut(s) 524
PsiI TTATAA 1 cut(s) 395
Psp6I CCWGG 2 cut(s) 42, 409
PspGI CCWGG 2 cut(s) 42, 409
PspN4I GGNNCC 2 cut(s) 6, 283
PspPI GGNCC 2 cut(s) 282, 814
PspXI VCTCGAGB 1 cut(s) 191
PstNI CAGNNNCTG 1 cut(s) 416
PsuI RGATCY 1 cut(s) 398
RsaI GTAC 1 cut(s) 41
RsaNI GTAC 1 cut(s) 40
SalI GTCGAC 1 cut(s) 335
SaqAI TTAA 4 cut(s) 27, 114, 326, 926
SatI GCNGC 2 cut(s) 519, 888
Sau3AI GATC 3 cut(s) 398, 681, 786
Sau96I GGNCC 2 cut(s) 282, 814
SchI GAGTC 1 cut(s) 524
ScrFI CCNGG 2 cut(s) 44, 411
SfaNI GCATC 1 cut(s) 286
Sfr274I CTCGAG 1 cut(s) 191
SinI GGWCC 2 cut(s) 282, 814
SlaI CTCGAG 1 cut(s) 191
SmlI CTYRAG 3 cut(s) 191, 575, 925
SmoI CTYRAG 3 cut(s) 191, 575, 925
Sse9I AATT 5 cut(s) 24, 59, 209, 626, 672
SspI AATATT 1 cut(s) 734
SspMI CTAG 1 cut(s) 870
StyD4I CCNGG 2 cut(s) 42, 409
StyI CCWWGG 1 cut(s) 739
TaaI ACNGT 3 cut(s) 78, 221, 290
TaiI ACGT 2 cut(s) 510, 540
TaqI TCGA 5 cut(s) 34, 192, 223, 336, 638
TasI AATT 5 cut(s) 24, 59, 209, 626, 672
TfiI GAWTC 1 cut(s) 777
Tru1I TTAA 4 cut(s) 27, 114, 326, 926
Tru9I TTAA 4 cut(s) 27, 114, 326, 926
TscAI CASTG 2 cut(s) 96, 861
TseFI GTSAC 1 cut(s) 47
TseI GCWGC 2 cut(s) 518, 887
Tsp45I GTSAC 1 cut(s) 47
TspDTI ATGAA 4 cut(s) 287, 656, 708, 772
TspRI CASTG 2 cut(s) 96, 861
Van91I CCANNNNNTGG 1 cut(s) 379
Vha464I CTTAAG 1 cut(s) 925
VpaK11BI GGWCC 2 cut(s) 282, 814
XapI RAATTY 2 cut(s) 626, 672
XhoI CTCGAG 1 cut(s) 191
XmiI GTMKAC 1 cut(s) 336
XmnI GAANNNNTTC 2 cut(s) 78, 249
XspI CTAG 1 cut(s) 870
ZraI GACGTC 1 cut(s) 538
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.