Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0005642

Physical Location & Seq

Genomic Coordinates Forward (+)

28784618 .. 28790296

Gene Structure 5679 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1201600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2646 bp

ATGGGATTTGAGAATTCCGCTGTCGTAGATGCTGTAAAAGAAGTAGGCCCTTCATTTGATGATGCATTGGATTATGTATTGAATGGTTGTAGTAGAAACAATCAGGGAGCATTGAGTAGTTCAAGATGCACCACAAGGAATGTGAAGGCTTTGGGGAAAAGGGCTTTTACTGCTCCGGGTCAAATTCGACAGTCTAGCATACTGGATCATTTTCATTCCACCGGTAGACCAAAACGGATTAAGACTGAGGTTGTACCTGACGTTTCTGTTTCTGGATCAAAACTATGTGCTTCTGTAGAACAATGTGAGAGACCTCCTTCTGGTGTGGATCATAGTGTTGAAGTCTTGACAGAATCGTCTGCATCGGATTGCCTGGACATTCAATCAGATTGGGAGAAGACAGCTAACAGTCTCTTGCAAAAGCATTTTGGTTATTCATCATTAAAAAGTTTCCAGAAGGAAGTCTTGGCTGCTTGGATGGCTCACCGAGATTCTCTTGTTCTTGCCGCAACAGGGTCTGGTAAATCTCTGTGCTTCCAGATTCCAGCACTGTTGACAGGGAAGGTGGTGGTGGTGATTTCACCGTTGATAAGCTTGATGCACGATCAATGCTTAAAGCTGGCAAAACATGGGGTTTCTGCTTGCTTTCTTGGATCTGGGCAACCAGATAGTACTGTGGAAAACAAAGCAATGACAGGGATGTACGACATAGTATATGTTTGTCCTGAAACTATCTTGAGACTGATAAAGCCACTTCAAAAATTAGCAGAAAATCGTGGAATCGCACTTTTCGCTATTGATGAAGTCCATTGTGTTTCGAAGTGGGGGCATGATTTTCGGCCTGACTACAGGAAATTGTCTGAGTTGCGAAAGAACTTCAGTGCTTGCAAGGTAAAATTCTTAAAATATGATATACCACTGATGGCATTGACTGCTACTGCCACAATTCAGGTCCGGGAAGACATTCTTAAGTCATTGTCCATGTCAAAGGAAACAAAGGTTGTGCTAACTTCCTTTTTCCGGCCAAATCTAAGATTTAAGGTACAACATAGTAGAACATCTGCATCAACTTATGAGGATGATTTCCGTGAATTGGTAGACATGTATACTGGAAAGAGAAAAACAGGTGAAAAGAAAAAAATGATCATGTCACAAGAAATAAACGACCTCCTGAATTGCTCTGCTAATAATACCATATCAGAGGCAGATAGCATTTCTCAGGATGATTTGGCCAATATTGAGGATGACTTCTCTGACAGAGATGATGAAGCAAGTTCATCCCAGGAAAATGGTTCAAGTGCTTCAAAGGGAAGGGAGCTGTCAGTTGACTACTTGGAAAATGACCTTGACATCTTCCAGAGCGTGAATGATTGGGATGTTAGCTGCGGTGAATTCTTGAGACAAACTGTTCGTGATGACTGGAATACTAGAAAAGAAACTAGATCTAATATAACTGATCTGCCAAATAAACCGGAAGAAAGACTAAGACTTGTGCAAGAGCCTTTACAGAAAGGGTCAACGATCATATATGTTCCTACTAGAAAGGGAACGTTGAGCATTGCAAATTATCTTTGTCGGTGCGGGGTGAAAGCTGCTGCCTATAATGCAGCGTTGCCAAAGTCGCACCTAAGGCAGGTCCACAAGATGTTTCATGAAAATACTTTAGAGGTTGTTGTTGCAACAATTGCATTTGGAATGGGAATTGACAAACTAAATGTCCGAAGAATCATCCACTATGGCTGGCCACAGAGTTTGGAAGCATACTATCAAGAAGCTGGTCGAGCGGGTAGAGATGGAAAATTAGCCGACTGCATTTTATTTGCAAACTTAACAAGAGCTCCGTCACTTTTGCCGAGTCGAAGAAGTGAAGAGCAGACAAAGCAAGCATATAAGATGTTATCAGATTGTTTCAGGTATGGAATGAATTCTTCATGCTGTCGGGCTAAAAAACTTGTGGAGTACTTCGGGGAGGATTTCAGTAATGAAAAGTGTCTCTTCTGTGATGTGTGTGTAAATGGACCCCCCGAACTGCAAAATTTGAGGAAGGAGACGGATCTCCTAATGCAGGTTATTGCTGCTCATCATTCAAGTAAATACAGGACCGGTTTATATGATGATGACATGGGTACTGATATTAGACATGAGAGGTATATGGGAAAGCTAAATCTCAGGATGATTGTCAGTAAAATAAGGGAGCAGTCTCAAGAATTTATGGCAACCGAACTTCTTTGGTGGCAAGGCCTTGTTCGAATCGTGGAAGGTCAAGGCTACGTCAAAGAGGGTGATACAAAGACACACGTTCAGCTAAAGTTCCCCGAGCTAACAGAACTTGGGTTGGAGTTTCTCAAATCCAAAACGGAGCAAACTTTCTACGTTCACCCTGAAGCGGATATGGTACTCTCTGCAAGCAGACCTAAATCTTTCTCAACTTTTTCGGACTGGGGCAAAGGCTGGGCTGATCCTGAGATTCGGCGTCAGCGGTTAGAGAGCATTCGAGGCAACAGAAAACCATTTAGCTCCGGAGGTAAAAGAGGAAGACGAAAGTCACGAAGGCAACGAAATAGTCCAAGTTTGAAAACTGCTCGAGGTAGAATAGAGGCCAAACTCTCCAATAAAAGTGTCCGGCCAAGGTCCTTGAATCATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000724 GO:0000725 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003824 GO:0004003 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0009378 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0032392 GO:0032508 GO:0033554 GO:0034641 GO:0042623 GO:0043138 GO:0043140 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0046483 GO:0050896 GO:0051276 GO:0051716 GO:0070035 GO:0071103 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:0140097 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD Helicase_C RecQ_Zn_bind

Protein Analysis

882

Amino Acids

99.26

Weight (kDa)

8.89

Isoelectric Point (pI)

45.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	150 - 319	5.8e-24	DEAD/DEAH box helicase
Helicase_C	PF00271	500 - 599	2.1e-15	Helicase conserved C-terminal domain
RecQ_Zn_bind	PF16124	620 - 670	1.5e-08	RecQ zinc-binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005642)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G27680 AT5G27680 AT5G27680 AT5G27680 AT5G27680 AT5G27680
fragaria_vesca	FvH4_6g06440 FvH4_6g06440 FvH4_6g06440 FvH4_6g06440
malus_domestica	MD04G1201600.v1.1
prunus_persica	Prupe.6G321800_v2.0.a1 Prupe.6G321800_v2.0.a1 Prupe.6G321800_v2.0.a1 Prupe.6G321800_v2.0.a1
pyrus_communis	pycom04g17780
rosa_chinensis	RchiOBHm_Chr3g0455521
rosa_laevigata	RLG00000025352
rosa_multiflora	Rmu_sc0005164.1_g000005 Rmu_sc0010428.1_g000001
rosa_roxburghii	Rroxscaffold_6G00423700 Rroxscaffold_6G00424190
rosa_rugosa	Rorug03G0008300
rosa_samantha	Rh3AG067700 Rh3BG070300 Rh3CG068600 Rh3DG070200
rosa_wichuraiana	Rw3G005380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	355
Acc36I	ACCTGC	2 cut(s)	1624, 2056
AccBSI	CCGCTC	1 cut(s)	1784
AccI	GTMKAC	3 cut(s)	226, 1098, 1106
AccIII	TCCGGA	1 cut(s)	2519
AciI	CCGC	7 cut(s)	18, 507, 1386, 1581, 1784, 2387, 2479
AclI	AACGTT	1 cut(s)	1550
AclWI	GGATC	6 cut(s)	213, 283, 336, 661, 2061, 2453
AcoI	YGGCCR	4 cut(s)	1022, 1230, 1742, 2624
AcsI	RAATTY	7 cut(s)	13, 183, 896, 1391, 1924, 2035, 2207
AcuI	CTGAAG	2 cut(s)	862, 2403
AcyI	GRCGYC	1 cut(s)	2473
AfaI	GTAC	7 cut(s)	255, 673, 704, 1044, 1961, 2128, 2397
AfiI	CCNNNNNNNGG	7 cut(s)	320, 513, 1020, 1093, 1633, 2065, 2386
AflII	CTTAAG	1 cut(s)	968
AflIII	ACRYGT	2 cut(s)	1101, 2296
AgeI	ACCGGT	2 cut(s)	221, 2102
AjnI	CCWGG	2 cut(s)	372, 1281
AjuI	GAANNNNNNNTTGG	2 cut(s)	449, 481
Alw21I	GWGCWC	1 cut(s)	1840
Alw26I	GTCTC	7 cut(s)	304, 416, 733, 1393, 1997, 2042, 2205
AlwI	GGATC	6 cut(s)	213, 283, 336, 661, 2061, 2453
AlwNI	CAGNNNCTG	1 cut(s)	518
Ama87I	CYCGRG	2 cut(s)	2315, 2583
Aor13HI	TCCGGA	1 cut(s)	2519
AoxI	GGCC	8 cut(s)	46, 839, 1022, 1230, 1742, 2239, 2598, 2624
ApeKI	GCWGC	6 cut(s)	470, 1383, 1592, 1595, 1607, 2075
ApoI	RAATTY	7 cut(s)	13, 183, 896, 1391, 1924, 2035, 2207
ArsI	GACNNNNNNTTYG	4 cut(s)	1392, 1424, 1701, 1733
AsiGI	ACCGGT	2 cut(s)	221, 2102
Asp700I	GAANNNNTTC	2 cut(s)	963, 1924
AspS9I	GGNCC	6 cut(s)	47, 952, 1636, 2018, 2100, 2631
AsuC2I	CCSGG	2 cut(s)	177, 956
AsuHPI	GGTGA	8 cut(s)	476, 573, 586, 1139, 1400, 1597, 2294, 2369
AsuII	TTCGAA	2 cut(s)	818, 2248
AvaI	CYCGRG	2 cut(s)	2315, 2583
AvaII	GGWCC	5 cut(s)	952, 1636, 2018, 2100, 2631
AxyI	CCTNAGG	1 cut(s)	1628
BalI	TGGCCA	2 cut(s)	1232, 1744
BanII	GRGCYC	1 cut(s)	1840
BbsI	GAAGAC	3 cut(s)	404, 966, 2542
Bbv12I	GWGCWC	1 cut(s)	1840
BbvI	GCAGC	6 cut(s)	457, 1370, 1579, 1582, 1619, 2062
BccI	CCATC	3 cut(s)	472, 916, 1787
BcgI	CGANNNNNNTGC	2 cut(s)	818, 852
BciT130I	CCWGG	2 cut(s)	374, 1283
BclI	TGATCA	1 cut(s)	1143
BcnI	CCSGG	2 cut(s)	177, 956
BcoDI	GTCTC	7 cut(s)	304, 416, 733, 1393, 1997, 2042, 2205
BfaI	CTAG	4 cut(s)	195, 1428, 1440, 1539
BfmI	CTRYAG	2 cut(s)	294, 847
BfrI	CTTAAG	1 cut(s)	968
BfuAI	ACCTGC	2 cut(s)	1624, 2056
BglII	AGATCT	1 cut(s)	1442
BisI	GCNGC	7 cut(s)	471, 507, 1384, 1593, 1596, 1608, 2076
BlsI	GCNGC	7 cut(s)	472, 508, 1385, 1594, 1597, 1609, 2077
BmcAI	AGTACT	2 cut(s)	673, 1961
Bme1390I	CCNGG	4 cut(s)	177, 374, 956, 1283
Bme18I	GGWCC	5 cut(s)	952, 1636, 2018, 2100, 2631
BmeT110I	CYCGRG	2 cut(s)	2315, 2583
BmgT120I	GGNCC	6 cut(s)	47, 952, 1636, 2018, 2100, 2631
BmiI	GGNNCC	1 cut(s)	2020
BmrFI	CCNGG	4 cut(s)	177, 374, 956, 1283
BmrI	ACTGGG	1 cut(s)	2449
BmsI	GCATC	6 cut(s)	19, 52, 116, 371, 588, 1073
BmuI	ACTGGG	1 cut(s)	2449
BoxI	GACNNNNGTC	1 cut(s)	2542
BpiI	GAAGAC	3 cut(s)	404, 966, 2542
Bpu14I	TTCGAA	2 cut(s)	818, 2248
BpuEI	CTTGAG	3 cut(s)	757, 1417, 2187
BpuMI	CCSGG	2 cut(s)	177, 956
BsaHI	GRCGYC	1 cut(s)	2473
BsaI	GGTCTC	1 cut(s)	304
BsaJI	CCNNGG	2 cut(s)	1281, 2627
BsaWI	WCCGGW	4 cut(s)	221, 1471, 2102, 2519
BsaXI	ACNNNNNCTCC	2 cut(s)	1822, 1852
Bsc4I	CCNNNNNNNGG	7 cut(s)	320, 513, 1020, 1093, 1633, 2065, 2386
Bse118I	RCCGGY	2 cut(s)	221, 2102
Bse1I	ACTGG	4 cut(s)	207, 1114, 1424, 2444
Bse21I	CCTNAGG	1 cut(s)	1628
Bse3DI	GCAATG	2 cut(s)	696, 1557
BseAI	TCCGGA	1 cut(s)	2519
BseBI	CCWGG	2 cut(s)	374, 1283
BseDI	CCNNGG	2 cut(s)	1281, 2627
BseGI	GGATG	9 cut(s)	483, 705, 1084, 1228, 1249, 1277, 1381, 1728, 2178
BseLI	CCNNNNNNNGG	7 cut(s)	320, 513, 1020, 1093, 1633, 2065, 2386
BseMI	GCAATG	2 cut(s)	696, 1557
BseMII	CTCAG	5 cut(s)	237, 852, 1232, 2182, 2454
BseNI	ACTGG	4 cut(s)	207, 1114, 1424, 2444
BseXI	GCAGC	6 cut(s)	457, 1370, 1579, 1582, 1619, 2062
BseYI	CCCAGC	1 cut(s)	2451
BshFI	GGCC	8 cut(s)	48, 841, 1024, 1232, 1744, 2241, 2600, 2626
BshTI	ACCGGT	2 cut(s)	221, 2102
BsiHKAI	GWGCWC	1 cut(s)	1840
BsiHKCI	CYCGRG	2 cut(s)	2315, 2583
BsiSI	CCGG	8 cut(s)	176, 222, 955, 1021, 1472, 2103, 2520, 2623
BslI	CCNNNNNNNGG	7 cut(s)	320, 513, 1020, 1093, 1633, 2065, 2386
BsmAI	GTCTC	7 cut(s)	304, 416, 733, 1393, 1997, 2042, 2205
BsmBI	CGTCTC	1 cut(s)	2042
BsmI	GAATGC	1 cut(s)	2490
BsnI	GGCC	8 cut(s)	48, 841, 1024, 1232, 1744, 2241, 2600, 2626
Bso31I	GGTCTC	1 cut(s)	304
BsoBI	CYCGRG	2 cut(s)	2315, 2583
Bsp119I	TTCGAA	2 cut(s)	818, 2248
Bsp1286I	GDGCHC	1 cut(s)	1840
Bsp13I	TCCGGA	1 cut(s)	2519
BspACI	CCGC	7 cut(s)	18, 507, 1386, 1581, 1784, 2387, 2479
BspANI	GGCC	8 cut(s)	48, 841, 1024, 1232, 1744, 2241, 2600, 2626
BspCNI	CTCAG	5 cut(s)	238, 853, 1231, 2181, 2455
BspEI	TCCGGA	1 cut(s)	2519
BspHI	TCATGA	1 cut(s)	1651
BspLI	GGNNCC	1 cut(s)	2020
BspMI	ACCTGC	2 cut(s)	1624, 2056
BspPI	GGATC	6 cut(s)	213, 283, 336, 661, 2061, 2453
BspQI	GCTCTTC	1 cut(s)	1863
BspT104I	TTCGAA	2 cut(s)	818, 2248
BspTI	CTTAAG	1 cut(s)	968
BspTNI	GGTCTC	1 cut(s)	304
BsrBI	CCGCTC	1 cut(s)	1784
BsrDI	GCAATG	2 cut(s)	696, 1557
BsrFI	RCCGGY	2 cut(s)	221, 2102
BsrI	ACTGG	4 cut(s)	207, 1114, 1424, 2444
BssAI	RCCGGY	2 cut(s)	221, 2102
BssECI	CCNNGG	2 cut(s)	1281, 2627
BssNAI	GTATAC	1 cut(s)	1107
BssNI	GRCGYC	1 cut(s)	2473
BssT1I	CCWWGG	1 cut(s)	2627
Bst1107I	GTATAC	1 cut(s)	1107
Bst2UI	CCWGG	2 cut(s)	374, 1283
Bst4CI	ACNGT	6 cut(s)	192, 410, 552, 585, 676, 1408
Bst6I	CTCTTC	2 cut(s)	1863, 2000
BstACI	GRCGYC	1 cut(s)	2473
BstAFI	CTTAAG	1 cut(s)	968
BstAPI	GCANNNNNTGC	2 cut(s)	932, 1685
BstBI	TTCGAA	2 cut(s)	818, 2248
BstC8I	GCNNGC	6 cut(s)	621, 643, 886, 1742, 1884, 2407
BstDEI	CTNAG	8 cut(s)	246, 861, 1031, 1218, 1484, 1628, 2168, 2463
BstENI	CCTNNNNNAGG	2 cut(s)	1631, 2063
BstF5I	GGATG	9 cut(s)	483, 705, 1084, 1228, 1249, 1277, 1381, 1728, 2178
BstMAI	GTCTC	7 cut(s)	304, 416, 733, 1393, 1997, 2042, 2205
BstNI	CCWGG	2 cut(s)	374, 1283
BstNSI	RCATGY	1 cut(s)	1105
BstPAI	GACNNNNGTC	1 cut(s)	2542
BstSCI	CCNGG	4 cut(s)	175, 372, 954, 1281
BstSFI	CTRYAG	2 cut(s)	294, 847
BstV1I	GCAGC	6 cut(s)	457, 1370, 1579, 1582, 1619, 2062
BstV2I	GAAGAC	3 cut(s)	404, 966, 2542
BstX2I	RGATCY	3 cut(s)	653, 1442, 2053
BstXI	CCANNNNNNTGG	1 cut(s)	1289
BstYI	RGATCY	3 cut(s)	653, 1442, 2053
BstZ17I	GTATAC	1 cut(s)	1107
Bsu36I	CCTNAGG	1 cut(s)	1628
BsuRI	GGCC	8 cut(s)	48, 841, 1024, 1232, 1744, 2241, 2600, 2626
BtsCI	GGATG	9 cut(s)	483, 705, 1084, 1228, 1249, 1277, 1381, 1728, 2178
BtsIMutI	CAGTG	3 cut(s)	548, 886, 917
BveI	ACCTGC	2 cut(s)	1624, 2056
Cac8I	GCNNGC	6 cut(s)	621, 643, 886, 1742, 1884, 2407
CaiI	CAGNNNCTG	1 cut(s)	518
CciI	TCATGA	1 cut(s)	1651
Cfr10I	RCCGGY	2 cut(s)	221, 2102
Cfr13I	GGNCC	6 cut(s)	47, 952, 1636, 2018, 2100, 2631
CseI	GACGC	1 cut(s)	2462
Csp6I	GTAC	7 cut(s)	254, 672, 703, 1043, 1960, 2127, 2396
CspAI	ACCGGT	2 cut(s)	221, 2102
CspCI	CAANNNNNGTGG	2 cut(s)	1734, 1769
CviAII	CATG	9 cut(s)	629, 830, 982, 1102, 1147, 1652, 1932, 2122, 2141
CviQI	GTAC	7 cut(s)	254, 672, 703, 1043, 1960, 2127, 2396
DdeI	CTNAG	8 cut(s)	246, 861, 1031, 1218, 1484, 1628, 2168, 2463
DrdI	GACNNNNNNGTC	1 cut(s)	355
DseDI	GACNNNNNNGTC	1 cut(s)	355
EaeI	YGGCCR	4 cut(s)	1022, 1230, 1742, 2624
Eam1104I	CTCTTC	2 cut(s)	1863, 2000
EarI	CTCTTC	2 cut(s)	1863, 2000
Ecl136II	GAGCTC	1 cut(s)	1838
Eco130I	CCWWGG	1 cut(s)	2627
Eco147I	AGGCCT	1 cut(s)	2241
Eco24I	GRGCYC	1 cut(s)	1840
Eco31I	GGTCTC	1 cut(s)	304
Eco47I	GGWCC	5 cut(s)	952, 1636, 2018, 2100, 2631
Eco53kI	GAGCTC	1 cut(s)	1838
Eco57I	CTGAAG	2 cut(s)	862, 2403
Eco81I	CCTNAGG	1 cut(s)	1628
Eco88I	CYCGRG	2 cut(s)	2315, 2583
EcoICRI	GAGCTC	1 cut(s)	1838
EcoNI	CCTNNNNNAGG	2 cut(s)	1631, 2063
EcoO109I	RGGNCCY	2 cut(s)	47, 2631
EcoRI	GAATTC	3 cut(s)	13, 1391, 1924
EcoRII	CCWGG	2 cut(s)	372, 1281
EcoT14I	CCWWGG	1 cut(s)	2627
EcoT22I	ATGCAT	1 cut(s)	67
EcoT38I	GRGCYC	1 cut(s)	1840
ErhI	CCWWGG	1 cut(s)	2627
Esp3I	CGTCTC	1 cut(s)	2042
FaeI	CATG	9 cut(s)	632, 833, 985, 1105, 1150, 1655, 1935, 2125, 2144
FalI	AAGNNNNNCTT	6 cut(s)	449, 481, 951, 983, 1979, 2011
FatI	CATG	9 cut(s)	628, 829, 981, 1101, 1146, 1651, 1931, 2121, 2140
FauI	CCCGC	2 cut(s)	1574, 1777
FbaI	TGATCA	1 cut(s)	1143
FblI	GTMKAC	3 cut(s)	226, 1098, 1106
Fnu4HI	GCNGC	7 cut(s)	471, 507, 1384, 1593, 1596, 1608, 2076
FokI	GGATG	9 cut(s)	490, 712, 1091, 1235, 1256, 1264, 1388, 1715, 2185
FriOI	GRGCYC	1 cut(s)	1840
Fsp4HI	GCNGC	7 cut(s)	471, 507, 1384, 1593, 1596, 1608, 2076
FspBI	CTAG	4 cut(s)	195, 1428, 1440, 1539
GluI	GCNGC	7 cut(s)	471, 507, 1384, 1593, 1596, 1608, 2076
GsaI	CCCAGC	1 cut(s)	2455
HaeIII	GGCC	8 cut(s)	48, 841, 1024, 1232, 1744, 2241, 2600, 2626
HapII	CCGG	8 cut(s)	176, 222, 955, 1021, 1472, 2103, 2520, 2623
HgaI	GACGC	1 cut(s)	2462
Hin1I	GRCGYC	1 cut(s)	2473
Hin1II	CATG	9 cut(s)	632, 833, 985, 1105, 1150, 1655, 1935, 2125, 2144
HincII	GTYRAC	3 cut(s)	555, 1327, 1518
HindII	GTYRAC	3 cut(s)	555, 1327, 1518
HindIII	AAGCTT	1 cut(s)	592
HinfI	GANTC	9 cut(s)	353, 491, 541, 780, 1725, 1855, 2250, 2467, 2638
HpaII	CCGG	8 cut(s)	176, 222, 955, 1021, 1472, 2103, 2520, 2623
HphI	GGTGA	8 cut(s)	476, 573, 586, 1139, 1400, 1597, 2294, 2369
Hpy166II	GTNNAC	8 cut(s)	227, 555, 1099, 1107, 1327, 1518, 1639, 2377
Hpy188I	TCNGA	8 cut(s)	367, 388, 862, 1201, 1255, 1721, 1903, 2437
Hpy8I	GTNNAC	8 cut(s)	227, 555, 1099, 1107, 1327, 1518, 1639, 2377
HpyAV	CCTTC	9 cut(s)	60, 139, 327, 451, 556, 1305, 2038, 2252, 2544
HpyCH4III	ACNGT	6 cut(s)	192, 410, 552, 585, 676, 1408
HpyCH4IV	ACGT	5 cut(s)	261, 1550, 2271, 2298, 2373
HpyF3I	CTNAG	8 cut(s)	246, 861, 1031, 1218, 1484, 1628, 2168, 2463
HpySE526I	ACGT	5 cut(s)	261, 1550, 2271, 2298, 2373
Hsp92I	GRCGYC	1 cut(s)	2473
Hsp92II	CATG	9 cut(s)	632, 833, 985, 1105, 1150, 1655, 1935, 2125, 2144
Kpn2I	TCCGGA	1 cut(s)	2519
Ksp22I	TGATCA	1 cut(s)	1143
LguI	GCTCTTC	1 cut(s)	1863
LmnI	GCTCC	7 cut(s)	107, 178, 1315, 1843, 2194, 2359, 2522
Lsp1109I	GCAGC	6 cut(s)	457, 1370, 1579, 1582, 1619, 2062
LweI	GCATC	6 cut(s)	19, 52, 116, 371, 588, 1073
MaeI	CTAG	4 cut(s)	195, 1428, 1440, 1539
MaeII	ACGT	5 cut(s)	261, 1550, 2271, 2298, 2373
MaeIII	GTNAC	3 cut(s)	1149, 1842, 2544
MbiI	CCGCTC	1 cut(s)	1784
MfeI	CAATTG	1 cut(s)	1683
MflI	RGATCY	3 cut(s)	653, 1442, 2053
MhlI	GDGCHC	1 cut(s)	1840
MlsI	TGGCCA	2 cut(s)	1232, 1744
MluNI	TGGCCA	2 cut(s)	1232, 1744
MlyI	GAGTC	1 cut(s)	1864
MmeI	TCCRAC	1 cut(s)	2316
Mox20I	TGGCCA	2 cut(s)	1232, 1744
Mph1103I	ATGCAT	1 cut(s)	67
MroI	TCCGGA	1 cut(s)	2519
MroXI	GAANNNNTTC	2 cut(s)	963, 1924
MscI	TGGCCA	2 cut(s)	1232, 1744
MseI	TTAA	7 cut(s)	240, 443, 614, 902, 969, 1038, 1829
Msp20I	TGGCCA	2 cut(s)	1232, 1744
MspA1I	CMGCKG	2 cut(s)	20, 2479
MspCI	CTTAAG	1 cut(s)	968
MspI	CCGG	8 cut(s)	176, 222, 955, 1021, 1472, 2103, 2520, 2623
MspR9I	CCNGG	4 cut(s)	177, 374, 956, 1283
MunI	CAATTG	1 cut(s)	1683
Mva1269I	GAATGC	1 cut(s)	2490
MvaI	CCWGG	2 cut(s)	374, 1283
NciI	CCSGG	2 cut(s)	177, 956
NlaIII	CATG	9 cut(s)	632, 833, 985, 1105, 1150, 1655, 1935, 2125, 2144
NlaIV	GGNNCC	1 cut(s)	2020
NmeAIII	GCCGAG	1 cut(s)	1878
NmuCI	GTSAC	3 cut(s)	1149, 1842, 2544
NsiI	ATGCAT	1 cut(s)	67
NspI	RCATGY	1 cut(s)	1105
NspV	TTCGAA	2 cut(s)	818, 2248
PaeR7I	CTCGAG	1 cut(s)	2583
PagI	TCATGA	1 cut(s)	1651
PceI	AGGCCT	1 cut(s)	2241
PciI	ACATGT	1 cut(s)	1101
PciSI	GCTCTTC	1 cut(s)	1863
PcsI	WCGNNNNNNNCGW	2 cut(s)	2545, 2554
PctI	GAATGC	1 cut(s)	2490
PdmI	GAANNNNTTC	2 cut(s)	963, 1924
PfeI	GAWTC	8 cut(s)	353, 491, 541, 780, 1725, 2250, 2467, 2638
PfoI	TCCNGGA	1 cut(s)	954
PinAI	ACCGGT	2 cut(s)	221, 2102
PkrI	GCNGC	7 cut(s)	472, 508, 1385, 1594, 1597, 1609, 2077
PleI	GAGTC	1 cut(s)	1863
PpsI	GAGTC	1 cut(s)	1863
PpuMI	RGGWCCY	1 cut(s)	2631
PscI	ACATGT	1 cut(s)	1101
PshAI	GACNNNNGTC	1 cut(s)	2542
Psp124BI	GAGCTC	1 cut(s)	1840
Psp1406I	AACGTT	1 cut(s)	1550
Psp5II	RGGWCCY	1 cut(s)	2631
Psp6I	CCWGG	2 cut(s)	372, 1281
PspFI	CCCAGC	1 cut(s)	2451
PspGI	CCWGG	2 cut(s)	372, 1281
PspN4I	GGNNCC	1 cut(s)	2020
PspPI	GGNCC	6 cut(s)	47, 952, 1636, 2018, 2100, 2631
PspPPI	RGGWCCY	1 cut(s)	2631
PspXI	VCTCGAGB	1 cut(s)	2583
PstNI	CAGNNNCTG	1 cut(s)	518
PsuI	RGATCY	3 cut(s)	653, 1442, 2053
RsaI	GTAC	7 cut(s)	255, 673, 704, 1044, 1961, 2128, 2397
RsaNI	GTAC	7 cut(s)	254, 672, 703, 1043, 1960, 2127, 2396
SacI	GAGCTC	1 cut(s)	1840
SapI	GCTCTTC	1 cut(s)	1863
SaqAI	TTAA	7 cut(s)	240, 443, 614, 902, 969, 1038, 1829
SatI	GCNGC	7 cut(s)	471, 507, 1384, 1593, 1596, 1608, 2076
Sau96I	GGNCC	6 cut(s)	47, 952, 1636, 2018, 2100, 2631
ScaI	AGTACT	2 cut(s)	673, 1961
SchI	GAGTC	1 cut(s)	1864
ScrFI	CCNGG	4 cut(s)	177, 374, 956, 1283
SduI	GDGCHC	1 cut(s)	1840
SfaNI	GCATC	6 cut(s)	19, 52, 116, 371, 588, 1073
SfcI	CTRYAG	2 cut(s)	294, 847
Sfr274I	CTCGAG	1 cut(s)	2583
SfuI	TTCGAA	2 cut(s)	818, 2248
SinI	GGWCC	5 cut(s)	952, 1636, 2018, 2100, 2631
SlaI	CTCGAG	1 cut(s)	2583
SmlI	CTYRAG	5 cut(s)	736, 968, 1396, 2202, 2583
SmoI	CTYRAG	5 cut(s)	736, 968, 1396, 2202, 2583
SseBI	AGGCCT	1 cut(s)	2241
SsiI	CCGC	7 cut(s)	18, 507, 1386, 1581, 1784, 2387, 2479
SspI	AATATT	1 cut(s)	1237
SspMI	CTAG	4 cut(s)	195, 1428, 1440, 1539
SstI	GAGCTC	1 cut(s)	1840
StuI	AGGCCT	1 cut(s)	2241
StyD4I	CCNGG	4 cut(s)	175, 372, 954, 1281
StyI	CCWWGG	1 cut(s)	2627
TaaI	ACNGT	6 cut(s)	192, 410, 552, 585, 676, 1408
TaiI	ACGT	5 cut(s)	264, 1553, 2274, 2301, 2376
TaqI	TCGA	7 cut(s)	187, 818, 1780, 1858, 2248, 2494, 2584
TatI	WGTACW	2 cut(s)	671, 1959
TauI	GCSGC	1 cut(s)	509
TfiI	GAWTC	8 cut(s)	353, 491, 541, 780, 1725, 2250, 2467, 2638
Tru1I	TTAA	7 cut(s)	240, 443, 614, 902, 969, 1038, 1829
Tru9I	TTAA	7 cut(s)	240, 443, 614, 902, 969, 1038, 1829
TscAI	CASTG	3 cut(s)	555, 886, 924
TseFI	GTSAC	3 cut(s)	1149, 1842, 2544
TseI	GCWGC	6 cut(s)	470, 1383, 1592, 1595, 1607, 2075
Tsp45I	GTSAC	3 cut(s)	1149, 1842, 2544
TspGWI	ACGGA	5 cut(s)	250, 1076, 1830, 2066, 2372
TspRI	CASTG	3 cut(s)	555, 886, 924
Vha464I	CTTAAG	1 cut(s)	968
VpaK11BI	GGWCC	5 cut(s)	952, 1636, 2018, 2100, 2631
XagI	CCTNNNNNAGG	2 cut(s)	1631, 2063
XapI	RAATTY	7 cut(s)	13, 183, 896, 1391, 1924, 2035, 2207
XceI	RCATGY	1 cut(s)	1105
XhoI	CTCGAG	1 cut(s)	2583
XmiI	GTMKAC	3 cut(s)	226, 1098, 1106
XmnI	GAANNNNTTC	2 cut(s)	963, 1924
XspI	CTAG	4 cut(s)	195, 1428, 1440, 1539
ZrmI	AGTACT	2 cut(s)	673, 1961
Zsp2I	ATGCAT	1 cut(s)	67

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.