Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0005642

Physical Location & Seq

Genomic Coordinates Reverse (-)

5197215 .. 5216228

Gene Structure 19014 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ42244

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2739 bp

ATGAGCTCAAGGGGCATTCTGCATACCATGAATGGCAATGGTGTCTCATCTGATGAAGTCATTGCCAAGCTGCTTGAAATGGGTTTCGAGAATTCCAGTGCCGTGGAGGCTGTAAAAGAAGTAGGGCCATCATTTGATGATGCATTGGAGTTTATATTGAATGGTTGTTGTAGAAGCAATCAGAGAGCATTGAGTAGTTCTAGATGTTCTACAAGCCAGGCAAAGGCTCCAGAGAAAAGAGCTTTGCCCTCCTCAAAACCGTTGGGTCAAATTCGGCAGTCTAGCATACTGGACCATTTCCAATCCTCGAGTGGACCTAAGCGGAGGAGGACTCATGTGTTACCTGATGTACCTGTATCAGAAATGGTGCGTGGTCCTGTAGAACAAGGTGTGGAACCTCTTTCTGGTGTGGATAGCATTGAAGTTTTATCAGAATCATCTCTGGTTGATTGCCTTGACATTCGATCAGATTGGGAGAAGAAAGTCAGCAGTCTCTTGCAAAAGCATTTTGGTTTTCTATCATTAAAACCTTTCCAGAAGGAGGTCTTAGCTGCTTGGTTGGCTCACCAAGATACACTTGTTCTTGCTGCTACAGGATCTGGAAAATCTTTGTGCTTCCAGATTCCAGCATTGTTGACAGGGAAAGTGGTTGTTGTGATTTCACCATTGATAAGCTTGATGCACGATCAGTGCTTAAAGCTGGCAAAACATGGGGTTTCTGCTTGCTTTCTTGGATCTGGACAACCTGATAGTACTGTGGAAAATAAAGCAATGAGAGGCATGTACAACATAATATACGTTTGCCCTGAGACAATATTAAGACTGATAAAACCACTCCAAAAGTTAGCAGAAATACGTGGGATTGCTCTTTTTGCAATTGATGAAGTGCATTGTGTTTCAAAGTGGGGCCATGATTTTCGGCCTGACTACAGGAGATTGTCTGAGTTGCGAAAGAACTTCAGTGCTAACAATTTAAAATTCTTAAAATTCGATATACCACTGATGGCATTAACTGCTACCGCCACAATTCAGGTTCGTGAAGACATTCTTAAGTCATTGTCCATGTCAAAGGAAACAAAAGTTGTGCTTACTTCATTTTTCCGGCCGAATCTAAGGTTCAGGGTACAGCATAGTAGAACATCAACATCATCATATGAGAATGATTTTTATGACTTGATAGACATGTATACGGGCAAGAGAGGAAAAGGTGAAAAGAAACAAATGATCATCTCAGAAGAAGTAAACGAAGCGCTGGACAGCTCATCTAACAGTAGTATGTCAGAGGCTGATTGCATTTCAGAGCATGATTTGGACAATATCGAGGATGGTAATTCCGACAGATATGATGAAGCAAGTTCACTCCAGGAAAATGGTTCAAGTGCTTCAAAGGGAAGGGAGCTGTCAGTTGACTACTTGGAAAATGATGTTGACATCTTTCAGAGTGTTAATGATTGGGATGTTAGCTGTGGTGAATTCTGTGGACTACCTCTTTGTGAGGACTTGAATACTAGAAAAGAAACTACATCGGAGGTACTTGATCCGCTGAGTCAACCAGAAGAAAGACTGAGACTTCTGCAACAGCCCCTAGAGAAAGGATCAACGATCATATATGTTCCTACGAGAAAACAAACGTTGAAAATTGCAACTTATCTTTCCAAGTGTGGAGTGAAGGCTGCTGCCTATAATGCAGGGTTGCCAAAATCACGTCTGAGGCAGGTCCACAAGATGTTTCATGAAAATACATTAGAGGTTGTTGTGGCAACAATTGCTTTTGGAATGGGAATTGACAAGTTAAATGTCCGAAGAATCATCCACTATGGTTGGCCACAGAGTTTGGAAGCATACTATCAAGAAGCTGGTCGAGCGGGAAGGGATGGAAAATTAGCTGACTGCATTTTATTTGCAAACCTAACAAGACCACCATCACTTTTACCGAGTCGAAGAAGTGAAGAGCAGACAAAGCAAGCATATAAAATGTTGTCTGATTGTTTCAGGTATGGAATGACTTCCTCATGCTGTCGGGCTAAAAAACTTGTGGAGTACTTTGGGGAGGATTTCAGTTATGAGAAGTGTCTCTCGTGTGATGTCTGTGTTAACGGACCTCCAGAAATGCAGAATTTCAGAAAGGAGGCAGATGTCTTGATGCAGGTTATTGCTGCTCAAAATAGACAAAGCAGATACAGGAATGATTCATATGATGATGCCATGAGTAGTGATATTAGACGTGAGAGCTATATGGGGAGACTCAATCTCAGAATGATTGTCAGTAAAATAAGAGAGCAGTCCCAACAGTTCATGGCAACTGAGCTGCTTTGGTGGCAAGGTCTTCTCCGAATTATGGAAGGTAAAGGGTTTGTCAGAGAGGGAGATGATAAGAGACATGTTCAATTGAAGTTCCCCAAGCTAACAGAACTCGGCCTGGAGTTTCTGCGATCTGAAAAGGAGAAAACTTTCTATGTTCACCCTGAAGCTGATATGCTGCTCTCAAAAAGCATGCCCAAATCTTTCTCAACTTTCTCAGAGTGGGGAAAAGGCTGGGCTGATCCTGAGATTCGACGCCAGCGGTTAGAGAGTTTTCGACACAACAGAAGCCCACAAAGCTCTTTTGGTAAAAGAGGAAAACGAAAATCACGAAAGAGAAAATCAAGTGCAAGTAGTCAAAGTGTTAGAACTGTTCGAGGTAGAATAGAGGCTAAACTCTCCAAACAAAGTATCAGACCAAGGTCATCGAATCAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000724 GO:0000725 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003824 GO:0004003 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0009378 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0032392 GO:0032508 GO:0033554 GO:0034641 GO:0042623 GO:0043138 GO:0043140 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0046483 GO:0050896 GO:0051276 GO:0051716 GO:0070035 GO:0071103 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:0140097 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD Helicase_C RecQ_Zn_bind

Protein Analysis

912

Amino Acids

102.93

Weight (kDa)

8.92

Isoelectric Point (pI)

56.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	177 - 346	9.6e-25	DEAD/DEAH box helicase
Helicase_C	PF00271	527 - 626	9.5e-16	Helicase conserved C-terminal domain
RecQ_Zn_bind	PF16124	647 - 697	1.1e-08	RecQ zinc-binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005642)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G27680 AT5G27680 AT5G27680 AT5G27680 AT5G27680 AT5G27680
fragaria_vesca	FvH4_6g06440 FvH4_6g06440 FvH4_6g06440 FvH4_6g06440
malus_domestica	MD04G1201600.v1.1
prunus_persica	Prupe.6G321800_v2.0.a1 Prupe.6G321800_v2.0.a1 Prupe.6G321800_v2.0.a1 Prupe.6G321800_v2.0.a1
pyrus_communis	pycom04g17780
rosa_chinensis	RchiOBHm_Chr3g0455521
rosa_laevigata	RLG00000025352
rosa_multiflora	Rmu_sc0005164.1_g000005 Rmu_sc0010428.1_g000001
rosa_roxburghii	Rroxscaffold_6G00423700 Rroxscaffold_6G00424190
rosa_rugosa	Rorug03G0008300
rosa_samantha	Rh3AG067700 Rh3BG070300 Rh3CG068600 Rh3DG070200
rosa_wichuraiana	Rw3G005380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	1705, 2137
AccBSI	CCGCTC	1 cut(s)	1865
AccI	GTMKAC	1 cut(s)	1187
AciI	CCGC	5 cut(s)	322, 1020, 1541, 1865, 2563
AclI	AACGTT	1 cut(s)	1631
AclWI	GGATC	5 cut(s)	604, 742, 1532, 1603, 2537
AcoI	YGGCCR	2 cut(s)	1103, 1823
AcsI	RAATTY	6 cut(s)	91, 270, 977, 986, 1472, 2116
AcuI	CTGAAG	2 cut(s)	943, 2487
AcyI	GRCGYC	1 cut(s)	2557
AfaI	GTAC	6 cut(s)	351, 754, 785, 1125, 1533, 2042
AfeI	AGCGCT	1 cut(s)	1251
AfiI	CCNNNNNNNGG	4 cut(s)	223, 404, 541, 2338
AflII	CTTAAG	1 cut(s)	1049
AflIII	ACRYGT	2 cut(s)	1182, 2380
AjiI	CACGTC	2 cut(s)	1706, 2225
AjnI	CCWGG	3 cut(s)	216, 1362, 2418
Alw21I	GWGCWC	1 cut(s)	8
Alw26I	GTCTC	7 cut(s)	49, 497, 803, 1561, 2078, 2236, 2371
AlwI	GGATC	5 cut(s)	604, 742, 1532, 1603, 2537
AlwNI	CAGNNNCTG	2 cut(s)	599, 1286
Ama87I	CYCGRG	1 cut(s)	307
Aor51HI	AGCGCT	1 cut(s)	1251
AoxI	GGCC	6 cut(s)	125, 907, 920, 1103, 1823, 2416
ApeKI	GCWGC	8 cut(s)	70, 551, 587, 1673, 1676, 2156, 2308, 2479
ApoI	RAATTY	6 cut(s)	91, 270, 977, 986, 1472, 2116
ArsI	GACNNNNNNTTYG	2 cut(s)	1473, 1505
Asp700I	GAANNNNTTC	3 cut(s)	1044, 2005, 2450
AspLEI	GCGC	1 cut(s)	1252
AspS9I	GGNCC	7 cut(s)	125, 292, 314, 374, 907, 1717, 2099
AsuHPI	GGTGA	5 cut(s)	557, 654, 1220, 1481, 2453
AvaI	CYCGRG	1 cut(s)	307
AvaII	GGWCC	5 cut(s)	292, 314, 374, 1717, 2099
BaeI	ACNNNNGTAYC	2 cut(s)	564, 597
BalI	TGGCCA	1 cut(s)	1825
BanII	GRGCYC	1 cut(s)	8
BauI	CACGAG	1 cut(s)	2077
BbsI	GAAGAC	2 cut(s)	1047, 2318
Bbv12I	GWGCWC	1 cut(s)	8
BbvI	GCAGC	8 cut(s)	57, 538, 574, 1660, 1663, 2143, 2295, 2466
BccI	CCATC	5 cut(s)	136, 997, 1319, 1868, 1930
BceAI	ACGGC	1 cut(s)	86
BciT130I	CCWGG	3 cut(s)	218, 1364, 2420
BclI	TGATCA	1 cut(s)	1224
BcoDI	GTCTC	7 cut(s)	49, 497, 803, 1561, 2078, 2236, 2371
BfaI	CTAG	4 cut(s)	201, 282, 1509, 1586
BfmI	CTRYAG	3 cut(s)	378, 591, 928
BfoI	RGCGCY	1 cut(s)	1253
BfrI	CTTAAG	1 cut(s)	1049
BfuAI	ACCTGC	2 cut(s)	1705, 2137
BglI	GCCNNNNNGGC	1 cut(s)	107
BisI	GCNGC	8 cut(s)	71, 552, 588, 1674, 1677, 2157, 2309, 2480
BlsI	GCNGC	8 cut(s)	72, 553, 589, 1675, 1678, 2158, 2310, 2481
BmcAI	AGTACT	2 cut(s)	754, 2042
Bme1390I	CCNGG	3 cut(s)	218, 1364, 2420
Bme18I	GGWCC	5 cut(s)	292, 314, 374, 1717, 2099
BmeT110I	CYCGRG	1 cut(s)	307
BmgBI	CACGTC	2 cut(s)	1706, 2225
BmgT120I	GGNCC	7 cut(s)	125, 292, 314, 374, 907, 1717, 2099
BmiI	GGNNCC	3 cut(s)	228, 396, 908
BmrFI	CCNGG	3 cut(s)	218, 1364, 2420
BmsI	GCATC	4 cut(s)	130, 669, 2133, 2191
BoxI	GACNNNNGTC	1 cut(s)	2722
BpiI	GAAGAC	2 cut(s)	1047, 2318
BplI	GAGNNNNNCTC	2 cut(s)	316, 348
BpmI	CTGGAG	4 cut(s)	213, 1346, 2088, 2441
Bpu10I	CCTNAGC	1 cut(s)	318
BsaAI	YACGTR	1 cut(s)	857
BsaHI	GRCGYC	1 cut(s)	2557
BsaJI	CCNNGG	2 cut(s)	102, 2720
Bsc4I	CCNNNNNNNGG	4 cut(s)	223, 404, 541, 2338
Bse1I	ACTGG	2 cut(s)	96, 294
Bse3DI	GCAATG	3 cut(s)	43, 60, 777
BseBI	CCWGG	3 cut(s)	218, 1364, 2420
BseDI	CCNNGG	2 cut(s)	102, 2720
BseGI	GGATG	4 cut(s)	1330, 1462, 1809, 1879
BseLI	CCNNNNNNNGG	4 cut(s)	223, 404, 541, 2338
BseMI	GCAATG	3 cut(s)	43, 60, 777
BseNI	ACTGG	2 cut(s)	96, 294
BseRI	GAGGAG	2 cut(s)	241, 340
BseX3I	CGGCCG	1 cut(s)	1103
BseXI	GCAGC	8 cut(s)	57, 538, 574, 1660, 1663, 2143, 2295, 2466
BseYI	CCCAGC	1 cut(s)	2535
Bsh1285I	CGRYCG	1 cut(s)	1106
BshFI	GGCC	6 cut(s)	127, 909, 922, 1105, 1825, 2418
BsiEI	CGRYCG	1 cut(s)	1106
BsiHKAI	GWGCWC	1 cut(s)	8
BsiHKCI	CYCGRG	1 cut(s)	307
BsiSI	CCGG	1 cut(s)	1102
BslFI	GGGAC	1 cut(s)	2269
BslI	CCNNNNNNNGG	4 cut(s)	223, 404, 541, 2338
BsmAI	GTCTC	7 cut(s)	49, 497, 803, 1561, 2078, 2236, 2371
BsmFI	GGGAC	1 cut(s)	2269
BsmI	GAATGC	1 cut(s)	15
BsnI	GGCC	6 cut(s)	127, 909, 922, 1105, 1825, 2418
BsoBI	CYCGRG	1 cut(s)	307
Bsp1286I	GDGCHC	1 cut(s)	8
Bsp1407I	TGTACA	1 cut(s)	783
BspACI	CCGC	5 cut(s)	322, 1020, 1541, 1865, 2563
BspANI	GGCC	6 cut(s)	127, 909, 922, 1105, 1825, 2418
BspHI	TCATGA	1 cut(s)	1732
BspLI	GGNNCC	3 cut(s)	228, 396, 908
BspMI	ACCTGC	2 cut(s)	1705, 2137
BspPI	GGATC	5 cut(s)	604, 742, 1532, 1603, 2537
BspQI	GCTCTTC	1 cut(s)	1944
BspTI	CTTAAG	1 cut(s)	1049
BsrBI	CCGCTC	1 cut(s)	1865
BsrDI	GCAATG	3 cut(s)	43, 60, 777
BsrGI	TGTACA	1 cut(s)	783
BsrI	ACTGG	2 cut(s)	96, 294
BssECI	CCNNGG	2 cut(s)	102, 2720
BssNAI	GTATAC	1 cut(s)	1188
BssNI	GRCGYC	1 cut(s)	2557
BssSI	CACGAG	1 cut(s)	2077
BssT1I	CCWWGG	1 cut(s)	2720
Bst1107I	GTATAC	1 cut(s)	1188
Bst2BI	CACGAG	1 cut(s)	2077
Bst2UI	CCWGG	3 cut(s)	218, 1364, 2420
Bst4CI	ACNGT	5 cut(s)	261, 757, 1271, 2292, 2674
Bst6I	CTCTTC	1 cut(s)	1944
BstACI	GRCGYC	1 cut(s)	2557
BstAFI	CTTAAG	1 cut(s)	1049
BstAPI	GCANNNNNTGC	2 cut(s)	1013, 1766
BstAUI	TGTACA	1 cut(s)	783
BstBAI	YACGTR	1 cut(s)	857
BstC8I	GCNNGC	5 cut(s)	702, 724, 1965, 2495, 2561
BstDSI	CCRYGG	1 cut(s)	102
BstF5I	GGATG	4 cut(s)	1330, 1462, 1809, 1879
BstH2I	RGCGCY	1 cut(s)	1253
BstHHI	GCGC	1 cut(s)	1252
BstMAI	GTCTC	7 cut(s)	49, 497, 803, 1561, 2078, 2236, 2371
BstMCI	CGRYCG	1 cut(s)	1106
BstNI	CCWGG	3 cut(s)	218, 1364, 2420
BstNSI	RCATGY	4 cut(s)	784, 1186, 2384, 2497
BstPAI	GACNNNNGTC	1 cut(s)	2722
BstSCI	CCNGG	3 cut(s)	216, 1362, 2418
BstSFI	CTRYAG	3 cut(s)	378, 591, 928
BstV1I	GCAGC	8 cut(s)	57, 538, 574, 1660, 1663, 2143, 2295, 2466
BstV2I	GAAGAC	2 cut(s)	1047, 2318
BstX2I	RGATCY	2 cut(s)	596, 734
BstXI	CCANNNNNNTGG	2 cut(s)	103, 1370
BstYI	RGATCY	2 cut(s)	596, 734
BstZ17I	GTATAC	1 cut(s)	1188
BstZI	CGGCCG	1 cut(s)	1103
BsuRI	GGCC	6 cut(s)	127, 909, 922, 1105, 1825, 2418
BtgI	CCRYGG	1 cut(s)	102
BtrI	CACGTC	2 cut(s)	1706, 2225
BtsCI	GGATG	4 cut(s)	1330, 1462, 1809, 1879
BtsIMutI	CAGTG	4 cut(s)	103, 695, 967, 998
BveI	ACCTGC	2 cut(s)	1705, 2137
Cac8I	GCNNGC	5 cut(s)	702, 724, 1965, 2495, 2561
CaiI	CAGNNNCTG	2 cut(s)	599, 1286
CciI	TCATGA	1 cut(s)	1732
CfoI	GCGC	1 cut(s)	1252
Cfr13I	GGNCC	7 cut(s)	125, 292, 314, 374, 907, 1717, 2099
CseI	GACGC	1 cut(s)	2565
Csp6I	GTAC	6 cut(s)	350, 753, 784, 1124, 1532, 2041
CspCI	CAANNNNNGTGG	4 cut(s)	1802, 1815, 1837, 1850
CviQI	GTAC	6 cut(s)	350, 753, 784, 1124, 1532, 2041
DraI	TTTAAA	1 cut(s)	975
EaeI	YGGCCR	2 cut(s)	1103, 1823
EagI	CGGCCG	1 cut(s)	1103
Eam1104I	CTCTTC	1 cut(s)	1944
EarI	CTCTTC	1 cut(s)	1944
Ecl136II	GAGCTC	1 cut(s)	6
EclXI	CGGCCG	1 cut(s)	1103
Eco130I	CCWWGG	1 cut(s)	2720
Eco24I	GRGCYC	1 cut(s)	8
Eco47I	GGWCC	5 cut(s)	292, 314, 374, 1717, 2099
Eco47III	AGCGCT	1 cut(s)	1251
Eco52I	CGGCCG	1 cut(s)	1103
Eco53kI	GAGCTC	1 cut(s)	6
Eco57I	CTGAAG	2 cut(s)	943, 2487
Eco88I	CYCGRG	1 cut(s)	307
EcoICRI	GAGCTC	1 cut(s)	6
EcoRI	GAATTC	2 cut(s)	91, 1472
EcoRII	CCWGG	3 cut(s)	216, 1362, 2418
EcoT14I	CCWWGG	1 cut(s)	2720
EcoT22I	ATGCAT	1 cut(s)	145
EcoT38I	GRGCYC	1 cut(s)	8
ErhI	CCWWGG	1 cut(s)	2720
FalI	AAGNNNNNCTT	8 cut(s)	530, 562, 561, 593, 1032, 1064, 1071, 1103
FaqI	GGGAC	1 cut(s)	2269
FauI	CCCGC	1 cut(s)	1858
FauNDI	CATATG	2 cut(s)	1153, 2194
FbaI	TGATCA	1 cut(s)	1224
FblI	GTMKAC	1 cut(s)	1187
Fnu4HI	GCNGC	8 cut(s)	71, 552, 588, 1674, 1677, 2157, 2309, 2480
FokI	GGATG	4 cut(s)	1337, 1469, 1796, 1886
FriOI	GRGCYC	1 cut(s)	8
Fsp4HI	GCNGC	8 cut(s)	71, 552, 588, 1674, 1677, 2157, 2309, 2480
FspBI	CTAG	4 cut(s)	201, 282, 1509, 1586
GlaI	GCGC	1 cut(s)	1251
GluI	GCNGC	8 cut(s)	71, 552, 588, 1674, 1677, 2157, 2309, 2480
GsaI	CCCAGC	1 cut(s)	2539
GsuI	CTGGAG	4 cut(s)	213, 1346, 2088, 2441
HaeII	RGCGCY	1 cut(s)	1253
HaeIII	GGCC	6 cut(s)	127, 909, 922, 1105, 1825, 2418
HapII	CCGG	1 cut(s)	1102
HgaI	GACGC	1 cut(s)	2565
HhaI	GCGC	1 cut(s)	1252
Hin1I	GRCGYC	1 cut(s)	2557
Hin6I	GCGC	1 cut(s)	1250
HinP1I	GCGC	1 cut(s)	1250
HincII	GTYRAC	5 cut(s)	636, 1408, 1429, 1550, 2095
HindII	GTYRAC	5 cut(s)	636, 1408, 1429, 1550, 2095
HindIII	AAGCTT	1 cut(s)	673
HpaI	GTTAAC	1 cut(s)	2095
HpaII	CCGG	1 cut(s)	1102
HphI	GGTGA	5 cut(s)	557, 654, 1220, 1481, 2453
Hpy99I	CGWCG	1 cut(s)	2559
HpyAV	CCTTC	5 cut(s)	532, 1386, 1663, 1863, 2336
HpyCH4III	ACNGT	5 cut(s)	261, 757, 1271, 2292, 2674
HpyCH4IV	ACGT	5 cut(s)	798, 856, 1631, 1705, 2224
HpySE526I	ACGT	5 cut(s)	798, 856, 1631, 1705, 2224
Hsp92I	GRCGYC	1 cut(s)	2557
HspAI	GCGC	1 cut(s)	1250
Ksp22I	TGATCA	1 cut(s)	1224
KspAI	GTTAAC	1 cut(s)	2095
LguI	GCTCTTC	1 cut(s)	1944
LmnI	GCTCC	2 cut(s)	232, 1396
Lsp1109I	GCAGC	8 cut(s)	57, 538, 574, 1660, 1663, 2143, 2295, 2466
LweI	GCATC	4 cut(s)	130, 669, 2133, 2191
MaeI	CTAG	4 cut(s)	201, 282, 1509, 1586
MaeII	ACGT	5 cut(s)	798, 856, 1631, 1705, 2224
MaeIII	GTNAC	1 cut(s)	339
MbiI	CCGCTC	1 cut(s)	1865
MboII	GAAGA	8 cut(s)	490, 1052, 1247, 1568, 1815, 1953, 1961, 2318
MfeI	CAATTG	3 cut(s)	876, 1764, 2387
MflI	RGATCY	2 cut(s)	596, 734
MhlI	GDGCHC	1 cut(s)	8
MlsI	TGGCCA	1 cut(s)	1825
MluNI	TGGCCA	1 cut(s)	1825
MlyI	GAGTC	4 cut(s)	325, 1555, 1945, 2238
MmeI	TCCRAC	1 cut(s)	1359
Mox20I	TGGCCA	1 cut(s)	1825
Mph1103I	ATGCAT	1 cut(s)	145
MroXI	GAANNNNTTC	3 cut(s)	1044, 2005, 2450
MscI	TGGCCA	1 cut(s)	1825
Msp20I	TGGCCA	1 cut(s)	1825
MspA1I	CMGCKG	2 cut(s)	1543, 2563
MspCI	CTTAAG	1 cut(s)	1049
MspI	CCGG	1 cut(s)	1102
MspR9I	CCNGG	3 cut(s)	218, 1364, 2420
MunI	CAATTG	3 cut(s)	876, 1764, 2387
Mva1269I	GAATGC	1 cut(s)	15
MvaI	CCWGG	3 cut(s)	218, 1364, 2420
NdeI	CATATG	2 cut(s)	1153, 2194
NlaIV	GGNNCC	3 cut(s)	228, 396, 908
NmeAIII	GCCGAG	1 cut(s)	2394
NsiI	ATGCAT	1 cut(s)	145
NspI	RCATGY	4 cut(s)	784, 1186, 2384, 2497
PaeI	GCATGC	1 cut(s)	2497
PaeR7I	CTCGAG	1 cut(s)	307
PagI	TCATGA	1 cut(s)	1732
PciI	ACATGT	2 cut(s)	1182, 2380
PciSI	GCTCTTC	1 cut(s)	1944
PcsI	WCGNNNNNNNCGW	1 cut(s)	2629
PctI	GAATGC	1 cut(s)	15
PdmI	GAANNNNTTC	3 cut(s)	1044, 2005, 2450
PfeI	GAWTC	7 cut(s)	434, 622, 1108, 1806, 2189, 2551, 2731
PfoI	TCCNGGA	1 cut(s)	1362
PkrI	GCNGC	8 cut(s)	72, 553, 589, 1675, 1678, 2158, 2310, 2481
PleI	GAGTC	4 cut(s)	325, 1554, 1944, 2238
PpsI	GAGTC	4 cut(s)	325, 1554, 1944, 2238
Ppu21I	YACGTR	1 cut(s)	857
PscI	ACATGT	2 cut(s)	1182, 2380
PshAI	GACNNNNGTC	1 cut(s)	2722
Psp124BI	GAGCTC	1 cut(s)	8
Psp1406I	AACGTT	1 cut(s)	1631
Psp6I	CCWGG	3 cut(s)	216, 1362, 2418
PspFI	CCCAGC	1 cut(s)	2535
PspGI	CCWGG	3 cut(s)	216, 1362, 2418
PspN4I	GGNNCC	3 cut(s)	228, 396, 908
PspPI	GGNCC	7 cut(s)	125, 292, 314, 374, 907, 1717, 2099
PspXI	VCTCGAGB	1 cut(s)	307
PstNI	CAGNNNCTG	2 cut(s)	599, 1286
PsuI	RGATCY	2 cut(s)	596, 734
RsaI	GTAC	6 cut(s)	351, 754, 785, 1125, 1533, 2042
RsaNI	GTAC	6 cut(s)	350, 753, 784, 1124, 1532, 2041
SacI	GAGCTC	1 cut(s)	8
SapI	GCTCTTC	1 cut(s)	1944
SatI	GCNGC	8 cut(s)	71, 552, 588, 1674, 1677, 2157, 2309, 2480
Sau96I	GGNCC	7 cut(s)	125, 292, 314, 374, 907, 1717, 2099
ScaI	AGTACT	2 cut(s)	754, 2042
SchI	GAGTC	4 cut(s)	325, 1555, 1945, 2238
ScrFI	CCNGG	3 cut(s)	218, 1364, 2420
SduI	GDGCHC	1 cut(s)	8
SfaNI	GCATC	4 cut(s)	130, 669, 2133, 2191
SfcI	CTRYAG	3 cut(s)	378, 591, 928
Sfr274I	CTCGAG	1 cut(s)	307
SinI	GGWCC	5 cut(s)	292, 314, 374, 1717, 2099
SlaI	CTCGAG	1 cut(s)	307
SmlI	CTYRAG	3 cut(s)	7, 307, 1049
SmoI	CTYRAG	3 cut(s)	7, 307, 1049
SphI	GCATGC	1 cut(s)	2497
SsiI	CCGC	5 cut(s)	322, 1020, 1541, 1865, 2563
SspI	AATATT	1 cut(s)	816
SspMI	CTAG	4 cut(s)	201, 282, 1509, 1586
SstI	GAGCTC	1 cut(s)	8
StyD4I	CCNGG	3 cut(s)	216, 1362, 2418
StyI	CCWWGG	1 cut(s)	2720
TaaI	ACNGT	5 cut(s)	261, 757, 1271, 2292, 2674
TaiI	ACGT	5 cut(s)	801, 859, 1634, 1708, 2227
TatI	WGTACW	3 cut(s)	752, 783, 2040
TfiI	GAWTC	7 cut(s)	434, 622, 1108, 1806, 2189, 2551, 2731
TscAI	CASTG	4 cut(s)	103, 695, 967, 1005
TseI	GCWGC	8 cut(s)	70, 551, 587, 1673, 1676, 2156, 2308, 2479
TspDTI	ATGAA	9 cut(s)	44, 69, 897, 1083, 1362, 1721, 1749, 2181, 2284
TspGWI	ACGGA	1 cut(s)	2112
TspRI	CASTG	4 cut(s)	103, 695, 967, 1005
Vha464I	CTTAAG	1 cut(s)	1049
VpaK11BI	GGWCC	5 cut(s)	292, 314, 374, 1717, 2099
XapI	RAATTY	6 cut(s)	91, 270, 977, 986, 1472, 2116
XbaI	TCTAGA	1 cut(s)	200
XceI	RCATGY	4 cut(s)	784, 1186, 2384, 2497
XcmI	CCANNNNNNNNNTGG	1 cut(s)	308
XhoI	CTCGAG	1 cut(s)	307
XmiI	GTMKAC	1 cut(s)	1187
XmnI	GAANNNNTTC	3 cut(s)	1044, 2005, 2450
XspI	CTAG	4 cut(s)	201, 282, 1509, 1586
ZrmI	AGTACT	2 cut(s)	754, 2042
Zsp2I	ATGCAT	1 cut(s)	145

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.