Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0005642

Physical Location & Seq

Genomic Coordinates Forward (+)

45383040 .. 45392249

Gene Structure 9210 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00424190.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3636 bp

ATGAGCACAAGGGGCATTCTGCATGCCATGAATGGCAATGGTGTCTCATCTGATGAAGTCATTGCGAAGCTGCTTGAAATGGGTTTCGAGAATTCCAGTGCCGTAGAGGCTGTAAAAGAAGTAGGGCCATCATTTGATGATGCATTGGAGTTTATATTGAATGGTTGTTGTAGAAGCAATCAGAGAGCATTGAGTAGTTCTAGATGTTCTACAAGCCAGGCAAAGGCTCCAGGGAAAAGAGCTTTGCCCTCCTCAAAACCATTGGGTCAAATTCGGCAGTCTAGCATACTGGACCATTTCCAATCCTCGAGTGGACCTAAGCAGAGGAGGACTCATGTGTTACCTGATGTGCCTGTATCAGAAATGGTGCATGTAGCACAATGTGTAAAACCTCTTTCTGGTGTGGATAGCATTGAAGTTTTATTAGAATCATCTCTGGTTGATTGCCTTGACATTCGATCAGATTGGGAGAAGAAAGTCAGCAGTCTCTTGCGAAAGCATTTTGGTTTTCTATCATTAAAACCTTTCCAGAAGGAGGTCTTAGCTGCTTGGTTGGCTCACCAAGATACACTTGTTCTTGCTGCAACAGGATCTGGAAAATCTTTGTGCTTCCAGATTCCAGCATTGTTGACAGGGAAAGTGGTTGTTGTGATTTCACCATTGATAAGCTTGATGCACGATCAGTGCTTAAAGCTGGCAAAACATGGGGTTTCTGCTTGCTTTCTTGGATCTGGACAACCTGATAGTACTGTGGAAAATAAAGCAATGAGAGGCATGTACAACATAATATACGTTTGCCCTGAGACAATATTAAGACTGATAAAACCACTCCAAAAGTTGACAGAAATACGTGGGATTGCTCTTTTTGCAATTGATGAAGTGCATTGTGTTTCAAAGTGGGGCCATGATTTTCGGCCTGACTACAGGAGATTGTCTGAGTTGCGAAAGAACTTCAGTGCTAGCAATTTAAAATTCTTAAAATTCGATATACCACTGATGGCATTAACTGCTACTGCCACAATTCAGGTTCGTGAAGACATTCTTAAGTCATTGTCCATGTCAAAGGAAACAAAAGTTGTGCTTACTTCATTTTTCCGGCCGAATCTAAGGTTCAGGGTACAGCATAGTAGAACATCAACATCATCATATGAGAATGATTTTTATGACTTGATAGACATGTATACGGGCAAGAGAGGAAAAGGTGACAAGAAACAAATGATCATCTCAGAAGAAGTAAACGAAGCGCTGGACAGCTCATCTAACAGTAGTATGTCAGAGGCTGATTGCATTTCAGAGCATGATTTGGACAATATCGAGGATGGTAATTCCGACAGATATGATGAAGCAAGTTCACTCCAGGAAAATGGTTCAAGTGCTTCAAAGGGAAGGGAGCTGTCAGTTGACTACTTGGAAAATGATGTTGACATCTTTCAGAGTGTTAATGATTGGGATGTTAGCTGTGGTGAATTCTGTGGACTACCTCTTTGTGAGGACTTGAATACTAGAAAAGAAACTACATCGGAGGTACTTGATCTGCCGAGTCAACCAGAAGAAAGACTGAGACTTCTGGAACAGCCCCTAGAGAAAGGATCAACTATCATATATGTTCCTACGAGAAAACAAACGTTGAAAATTGCAACTTATCTTTCCAAGTGTGGAGTGAAGGCTGCTGCCTATAATGCAGGGTTGCCAAAATCACGTCTGAGGCAGGTCCACAAGATGTTTCATGAAAATACATTAGAGGTTGTTGTGGCAACAATTGCTTTTGGAATGGGAATTGACAAGTTAAATGTCCGAAGAATCATCCACTATGGTTGGCCACAGAGTTTGGAAGCATACTATCAAGAAGCTGGTCGAGCAGGAAGGGATGGAAAATTAGCTGACTGCATTTTATTTGCAAACCTAACAAGACCACCATCACTTTTACCGAGTCGAAGAAGTGAAGAGCAGACAAAGCAAGCATATAAAATGTTGTCTGATTGTTTCAGGTATGGAATGACTTCCTCATGCTGTCGGGCTAAAAAACTTGTGGAGTACTTTGGGGAGCATTTCAGTTATGAGAAGTGTCTCTCGTGTGATGTCTGTGTTAACGGACCTCTAGAAATGCAGAATTTCAGAAAGGAGGCAGATGTCTTGATGCAGGTTATTGCTGCTCATGATAGACAAAGCAGATACAGGAATGATTCATATGATGATGCCATGAGTAGTGATATTAGACGTGAGAGCTCTATGGGGAGACTCAATCTCAGAATGATTGTCAGTAAAATAAGAGAGCAGTCCCAACAGTTCATGGCAACTGAGCTGCTTTGGTGGCAAGGTCTTCTCCGAATTATGGAAGGTAAAGGGTTCGTCAGAGAGGGAGATGATAAGAGACATGTTCAATTGAAGTTTCCCAAGCTAACAGAACTCGGCCTGGAGTTTCTGCGATCTGAAAAGGAGAAAACTTTCTATGTTCACCCTGAAGCTGATATGCTGCTCTCAAAAAACATGCCCAAATCTTTCTCGACTTTCTCAGAGTGGGGAAAAGGCTGGGCTGATCCTGAGATTCGACGCCAGCGGTTAGAGAGTTTTCGACACAATAGAAGCCCACAAAGCTCTTTTGGTAAAAGAGGAAAACGAAAATCACGAAAGAGAAAATCAAGTGCAAGTAGTCAAAGTGTTAGAACTGTTCGAGTGATCAAGTTAACGTGTGCTGGCATGGGACTGAAAATGGAGTTGGATAGACTTAGCAGTTTACCAGATGATGTTACAGAAAAGATTTTGGAACGTTTGCCGCTTAAGGAGGCTGTAAGGACAAGTATTTTATCCAGAAAGTGGAGGTGTTTGCTAAACCCTCCTTCCACATTCAAAGGCTTTGGCAGCTTGAAGAGCCTTGATCTTTGGTCTGTTACTTTGGCCCAGGATGTGTTTGAGAACTTGATTGGTTGCAGTCCTCTGCTTGAGATACTGAAATTGCAAAGATGTGATGGTTTCACCAATCTCAAGATTGATGCACCGAAGCTCTGGTTTATTTACATAATAGGGGCCTTTGAAGATTTTAACCTCGTGGGTTCCTCAAATCTTGTGGATGCTTCCTTTGATTTGCAGGTCATTGTTGATCAAAGAGGGAATTGTAGTAGTTTTGGGAATTTGCTTAAGTTTTTTGATCACCAGCTGCCTCATATTCGAAGGCTCACAATAAAGAGGAACTTTCTAAAATACTTGTCTATTGGTGCCTTGCCAGAGAAGCTCCCTAAACCATGCCAATATCTCAATTTTCTTTCTTTAGACATGAACTTTGACAATCCGGATGAAATTTCAACTGTCTTATGCCTTCTGAGAAGCTCTCCTGCTCTAAAAGAACTGAAAATTTTGGTGGACCCTGAAAAGGATCACGCTGCTGTGGGAGAAGTGGAATCTTGTTTATATGACAACTGCAACTGTGCATACACTCAACTGCGGCTTGTGGAAATAACCAAGATCTCTGGTGTAAAAGCTCAACTAGATTTCATCGAATCTCTGCTTCTAAGTTCACCTGTGCTTGAGAGAATGACTGTTCAACCTGCTTCTGTCGATGGTTTTTTGAAGCTAGTAAAAGATTTACTTCTGTTTAAGCGTGATTCAAAGCGTGCAGAGATAATGGTTTTGGACCCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000724 GO:0000725 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003824 GO:0004003 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0009378 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0032392 GO:0032508 GO:0033554 GO:0034641 GO:0042623 GO:0043138 GO:0043140 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0046483 GO:0050896 GO:0051276 GO:0051716 GO:0070035 GO:0071103 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:0140097 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD Helicase_C RecQ_Zn_bind

Protein Analysis

1211

Amino Acids

136.6

Weight (kDa)

8.59

Isoelectric Point (pI)

48.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	175 - 344	1.3e-24	DEAD/DEAH box helicase
Helicase_C	PF00271	525 - 624	1.4e-15	Helicase conserved C-terminal domain
RecQ_Zn_bind	PF16124	645 - 695	1.4e-08	RecQ zinc-binding
F-box	PF00646	909 - 944	3.7e-08	F-box domain
LRR_At5g56370	PF24758	938 - 1117	9.4e-11	FBD-associated F-box protein At5g56370, LRR repeats
LRR_At1g61320_AtMIF1	PF23622	940 - 1180	2.3e-10	At1g61320/AtMIF1, LRR domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Publication-ready

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Orthologous Genes (Group: OG0005642)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G27680 AT5G27680 AT5G27680 AT5G27680 AT5G27680 AT5G27680
fragaria_vesca	FvH4_6g06440 FvH4_6g06440 FvH4_6g06440 FvH4_6g06440
malus_domestica	MD04G1201600.v1.1
prunus_persica	Prupe.6G321800_v2.0.a1 Prupe.6G321800_v2.0.a1 Prupe.6G321800_v2.0.a1 Prupe.6G321800_v2.0.a1
pyrus_communis	pycom04g17780
rosa_chinensis	RchiOBHm_Chr3g0455521
rosa_laevigata	RLG00000025352
rosa_multiflora	Rmu_sc0005164.1_g000005 Rmu_sc0010428.1_g000001
rosa_roxburghii	Rroxscaffold_6G00423700 Rroxscaffold_6G00424190
rosa_rugosa	Rorug03G0008300
rosa_samantha	Rh3AG067700 Rh3BG070300 Rh3CG068600 Rh3DG070200
rosa_wichuraiana	Rw3G005380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	4 cut(s)	1699, 2131, 3076, 3550
AccB1I	GGYRCC	1 cut(s)	3212
AccB7I	CCANNNNNTGG	1 cut(s)	2814
AccI	GTMKAC	1 cut(s)	1181
AccIII	TCCGGA	1 cut(s)	3286
AciI	CCGC	3 cut(s)	2557, 2774, 3439
AclI	AACGTT	2 cut(s)	1625, 2767
AclWI	GGATC	5 cut(s)	598, 736, 1597, 2531, 3378
AcoI	YGGCCR	2 cut(s)	1097, 1817
AcsI	RAATTY	9 cut(s)	91, 270, 971, 980, 1466, 2110, 3127, 3294, 3348
AcuI	CTGAAG	2 cut(s)	937, 2481
AcyI	GRCGYC	1 cut(s)	2551
AdeI	CACNNNGTG	1 cut(s)	383
AfaI	GTAC	5 cut(s)	748, 779, 1119, 1527, 2036
AfeI	AGCGCT	1 cut(s)	1245
AfiI	CCNNNNNNNGG	6 cut(s)	223, 398, 535, 2332, 2814, 3367
AflII	CTTAAG	3 cut(s)	1043, 2777, 3134
AflIII	ACRYGT	3 cut(s)	1176, 2374, 2687
AjiI	CACGTC	2 cut(s)	1700, 2219
AjnI	CCWGG	5 cut(s)	216, 229, 1356, 2412, 2898
AjuI	GAANNNNNNNTTGG	2 cut(s)	2699, 2731
Alw21I	GWGCWC	2 cut(s)	8, 2228
Alw26I	GTCTC	7 cut(s)	49, 491, 797, 1555, 2072, 2230, 2365
AlwI	GGATC	5 cut(s)	598, 736, 1597, 2531, 3378
AlwNI	CAGNNNCTG	2 cut(s)	593, 1280
Ama87I	CYCGRG	1 cut(s)	307
Aor13HI	TCCGGA	1 cut(s)	3286
Aor51HI	AGCGCT	1 cut(s)	1245
AoxI	GGCC	8 cut(s)	125, 901, 914, 1097, 1817, 2410, 2895, 3024
ApoI	RAATTY	9 cut(s)	91, 270, 971, 980, 1466, 2110, 3127, 3294, 3348
ArsI	GACNNNNNNTTYG	4 cut(s)	1467, 1499, 3260, 3292
Asp700I	GAANNNNTTC	3 cut(s)	1038, 1999, 2444
AspLEI	GCGC	1 cut(s)	1246
AsuHPI	GGTGA	8 cut(s)	551, 648, 1214, 1475, 2447, 2965, 3140, 3504
AsuII	TTCGAA	1 cut(s)	3166
AsuNHI	GCTAGC	1 cut(s)	959
AvaI	CYCGRG	1 cut(s)	307
AvaII	GGWCC	6 cut(s)	292, 314, 1711, 2093, 3358, 3628
BaeI	ACNNNNGTAYC	2 cut(s)	558, 591
BalI	TGGCCA	1 cut(s)	1819
BanI	GGYRCC	1 cut(s)	3212
BanII	GRGCYC	1 cut(s)	2228
BauI	CACGAG	2 cut(s)	2071, 3044
BbsI	GAAGAC	2 cut(s)	1041, 2312
Bbv12I	GWGCWC	2 cut(s)	8, 2228
BccI	CCATC	7 cut(s)	136, 991, 1313, 1862, 1924, 2960, 3548
BceAI	ACGGC	1 cut(s)	86
BciT130I	CCWGG	5 cut(s)	218, 231, 1358, 2414, 2900
BclI	TGATCA	4 cut(s)	1218, 2676, 3097, 3145
BcoDI	GTCTC	7 cut(s)	49, 491, 797, 1555, 2072, 2230, 2365
BfaI	CTAG	8 cut(s)	201, 282, 960, 1503, 1580, 2099, 3482, 3569
BfmI	CTRYAG	1 cut(s)	922
BfoI	RGCGCY	1 cut(s)	1247
BfrI	CTTAAG	3 cut(s)	1043, 2777, 3134
BfuAI	ACCTGC	4 cut(s)	1699, 2131, 3076, 3550
BglI	GCCNNNNNGGC	1 cut(s)	107
BglII	AGATCT	1 cut(s)	3459
BmcAI	AGTACT	2 cut(s)	748, 2036
Bme1390I	CCNGG	5 cut(s)	218, 231, 1358, 2414, 2900
Bme18I	GGWCC	6 cut(s)	292, 314, 1711, 2093, 3358, 3628
BmeT110I	CYCGRG	1 cut(s)	307
BmgBI	CACGTC	2 cut(s)	1700, 2219
BmiI	GGNNCC	7 cut(s)	228, 902, 3025, 3052, 3214, 3360, 3630
BmrFI	CCNGG	5 cut(s)	218, 231, 1358, 2414, 2900
BmsI	GCATC	6 cut(s)	130, 663, 2127, 2185, 2980, 3058
BmtI	GCTAGC	1 cut(s)	963
BpiI	GAAGAC	2 cut(s)	1041, 2312
BplI	GAGNNNNNCTC	4 cut(s)	316, 348, 3310, 3342
BpmI	CTGGAG	3 cut(s)	213, 1340, 2435
Bpu10I	CCTNAGC	1 cut(s)	318
Bpu14I	TTCGAA	1 cut(s)	3166
BpuEI	CTTGAG	3 cut(s)	2960, 2966, 3542
BsaAI	YACGTR	1 cut(s)	851
BsaHI	GRCGYC	1 cut(s)	2551
BsaJI	CCNNGG	2 cut(s)	230, 2898
BsaWI	WCCGGW	1 cut(s)	3286
Bsc4I	CCNNNNNNNGG	6 cut(s)	223, 398, 535, 2332, 2814, 3367
Bse1I	ACTGG	2 cut(s)	96, 294
Bse3DI	GCAATG	3 cut(s)	43, 60, 771
BseAI	TCCGGA	1 cut(s)	3286
BseBI	CCWGG	5 cut(s)	218, 231, 1358, 2414, 2900
BseDI	CCNNGG	2 cut(s)	230, 2898
BseGI	GGATG	7 cut(s)	1324, 1456, 1803, 1873, 2908, 3073, 3295
BseLI	CCNNNNNNNGG	6 cut(s)	223, 398, 535, 2332, 2814, 3367
BseMI	GCAATG	3 cut(s)	43, 60, 771
BseNI	ACTGG	2 cut(s)	96, 294
BseRI	GAGGAG	2 cut(s)	241, 340
BseX3I	CGGCCG	1 cut(s)	1097
BseYI	CCCAGC	1 cut(s)	2529
BsgI	GTGCAG	1 cut(s)	3630
Bsh1285I	CGRYCG	1 cut(s)	1100
BshFI	GGCC	8 cut(s)	127, 903, 916, 1099, 1819, 2412, 2897, 3026
BshNI	GGYRCC	1 cut(s)	3212
BsiEI	CGRYCG	1 cut(s)	1100
BsiHKAI	GWGCWC	2 cut(s)	8, 2228
BsiHKCI	CYCGRG	1 cut(s)	307
BsiSI	CCGG	2 cut(s)	1096, 3287
BslFI	GGGAC	2 cut(s)	2263, 2715
BslI	CCNNNNNNNGG	6 cut(s)	223, 398, 535, 2332, 2814, 3367
BsmAI	GTCTC	7 cut(s)	49, 491, 797, 1555, 2072, 2230, 2365
BsmFI	GGGAC	2 cut(s)	2263, 2715
BsmI	GAATGC	1 cut(s)	15
BsnI	GGCC	8 cut(s)	127, 903, 916, 1099, 1819, 2412, 2897, 3026
BsoBI	CYCGRG	1 cut(s)	307
Bsp119I	TTCGAA	1 cut(s)	3166
Bsp1286I	GDGCHC	2 cut(s)	8, 2228
Bsp13I	TCCGGA	1 cut(s)	3286
Bsp1407I	TGTACA	1 cut(s)	777
BspACI	CCGC	3 cut(s)	2557, 2774, 3439
BspANI	GGCC	8 cut(s)	127, 903, 916, 1099, 1819, 2412, 2897, 3026
BspEI	TCCGGA	1 cut(s)	3286
BspHI	TCATGA	2 cut(s)	1726, 2155
BspLI	GGNNCC	7 cut(s)	228, 902, 3025, 3052, 3214, 3360, 3630
BspMI	ACCTGC	4 cut(s)	1699, 2131, 3076, 3550
BspOI	GCTAGC	1 cut(s)	963
BspPI	GGATC	5 cut(s)	598, 736, 1597, 2531, 3378
BspQI	GCTCTTC	2 cut(s)	1938, 2861
BspT104I	TTCGAA	1 cut(s)	3166
BspT107I	GGYRCC	1 cut(s)	3212
BspTI	CTTAAG	3 cut(s)	1043, 2777, 3134
BsrDI	GCAATG	3 cut(s)	43, 60, 771
BsrGI	TGTACA	1 cut(s)	777
BsrI	ACTGG	2 cut(s)	96, 294
BssECI	CCNNGG	2 cut(s)	230, 2898
BssNAI	GTATAC	1 cut(s)	1182
BssNI	GRCGYC	1 cut(s)	2551
BssSI	CACGAG	2 cut(s)	2071, 3044
Bst1107I	GTATAC	1 cut(s)	1182
Bst2BI	CACGAG	2 cut(s)	2071, 3044
Bst2UI	CCWGG	5 cut(s)	218, 231, 1358, 2414, 2900
Bst4CI	ACNGT	7 cut(s)	751, 1265, 2286, 2668, 3304, 3422, 3535
Bst6I	CTCTTC	2 cut(s)	1938, 2861
BstACI	GRCGYC	1 cut(s)	2551
BstAFI	CTTAAG	3 cut(s)	1043, 2777, 3134
BstAPI	GCANNNNNTGC	2 cut(s)	1007, 1760
BstAUI	TGTACA	1 cut(s)	777
BstBAI	YACGTR	1 cut(s)	851
BstBI	TTCGAA	1 cut(s)	3166
BstC8I	GCNNGC	8 cut(s)	24, 696, 718, 961, 1959, 2555, 2695, 3609
BstF5I	GGATG	7 cut(s)	1324, 1456, 1803, 1873, 2908, 3073, 3295
BstH2I	RGCGCY	1 cut(s)	1247
BstHHI	GCGC	1 cut(s)	1246
BstMAI	GTCTC	7 cut(s)	49, 491, 797, 1555, 2072, 2230, 2365
BstMCI	CGRYCG	1 cut(s)	1100
BstNI	CCWGG	5 cut(s)	218, 231, 1358, 2414, 2900
BstNSI	RCATGY	6 cut(s)	26, 374, 778, 1180, 2378, 2491
BstSCI	CCNGG	5 cut(s)	216, 229, 1356, 2412, 2898
BstSFI	CTRYAG	1 cut(s)	922
BstV2I	GAAGAC	2 cut(s)	1041, 2312
BstX2I	RGATCY	3 cut(s)	590, 728, 3459
BstXI	CCANNNNNNTGG	1 cut(s)	1364
BstYI	RGATCY	3 cut(s)	590, 728, 3459
BstZ17I	GTATAC	1 cut(s)	1182
BstZI	CGGCCG	1 cut(s)	1097
BsuRI	GGCC	8 cut(s)	127, 903, 916, 1099, 1819, 2412, 2897, 3026
BtrI	CACGTC	2 cut(s)	1700, 2219
BtsCI	GGATG	7 cut(s)	1324, 1456, 1803, 1873, 2908, 3073, 3295
BtsIMutI	CAGTG	4 cut(s)	103, 689, 961, 992
BveI	ACCTGC	4 cut(s)	1699, 2131, 3076, 3550
Cac8I	GCNNGC	8 cut(s)	24, 696, 718, 961, 1959, 2555, 2695, 3609
CaiI	CAGNNNCTG	2 cut(s)	593, 1280
CciI	TCATGA	2 cut(s)	1726, 2155
CfoI	GCGC	1 cut(s)	1246
CseI	GACGC	1 cut(s)	2559
Csp6I	GTAC	5 cut(s)	747, 778, 1118, 1526, 2035
CspCI	CAANNNNNGTGG	6 cut(s)	1796, 1809, 1831, 1844, 3045, 3080
CviQI	GTAC	5 cut(s)	747, 778, 1118, 1526, 2035
DraI	TTTAAA	1 cut(s)	969
DraIII	CACNNNGTG	1 cut(s)	383
EaeI	YGGCCR	2 cut(s)	1097, 1817
EagI	CGGCCG	1 cut(s)	1097
Eam1104I	CTCTTC	2 cut(s)	1938, 2861
EarI	CTCTTC	2 cut(s)	1938, 2861
Ecl136II	GAGCTC	1 cut(s)	2226
EclXI	CGGCCG	1 cut(s)	1097
Eco24I	GRGCYC	1 cut(s)	2228
Eco47I	GGWCC	6 cut(s)	292, 314, 1711, 2093, 3358, 3628
Eco47III	AGCGCT	1 cut(s)	1245
Eco52I	CGGCCG	1 cut(s)	1097
Eco53kI	GAGCTC	1 cut(s)	2226
Eco57I	CTGAAG	2 cut(s)	937, 2481
Eco88I	CYCGRG	1 cut(s)	307
EcoICRI	GAGCTC	1 cut(s)	2226
EcoO109I	RGGNCCY	1 cut(s)	3024
EcoRI	GAATTC	2 cut(s)	91, 1466
EcoRII	CCWGG	5 cut(s)	216, 229, 1356, 2412, 2898
EcoT22I	ATGCAT	1 cut(s)	145
EcoT38I	GRGCYC	1 cut(s)	2228
FaqI	GGGAC	2 cut(s)	2263, 2715
FauNDI	CATATG	2 cut(s)	1147, 2188
FbaI	TGATCA	4 cut(s)	1218, 2676, 3097, 3145
FblI	GTMKAC	1 cut(s)	1181
FokI	GGATG	7 cut(s)	1331, 1463, 1790, 1880, 2915, 3080, 3302
FriOI	GRGCYC	1 cut(s)	2228
FspBI	CTAG	8 cut(s)	201, 282, 960, 1503, 1580, 2099, 3482, 3569
GlaI	GCGC	1 cut(s)	1245
GsaI	CCCAGC	1 cut(s)	2533
GsuI	CTGGAG	3 cut(s)	213, 1340, 2435
HaeII	RGCGCY	1 cut(s)	1247
HaeIII	GGCC	8 cut(s)	127, 903, 916, 1099, 1819, 2412, 2897, 3026
HapII	CCGG	2 cut(s)	1096, 3287
HgaI	GACGC	1 cut(s)	2559
HhaI	GCGC	1 cut(s)	1246
Hin1I	GRCGYC	1 cut(s)	2551
Hin6I	GCGC	1 cut(s)	1244
HinP1I	GCGC	1 cut(s)	1244
HincII	GTYRAC	7 cut(s)	630, 840, 1402, 1423, 1544, 2089, 2685
HindII	GTYRAC	7 cut(s)	630, 840, 1402, 1423, 1544, 2089, 2685
HindIII	AAGCTT	1 cut(s)	667
HpaI	GTTAAC	2 cut(s)	2089, 2685
HpaII	CCGG	2 cut(s)	1096, 3287
HphI	GGTGA	8 cut(s)	551, 648, 1214, 1475, 2447, 2965, 3140, 3504
Hpy99I	CGWCG	1 cut(s)	2553
HpyAV	CCTTC	8 cut(s)	526, 1380, 1657, 1857, 2330, 2847, 3162, 3323
HpyCH4III	ACNGT	7 cut(s)	751, 1265, 2286, 2668, 3304, 3422, 3535
HpyCH4IV	ACGT	7 cut(s)	792, 850, 1625, 1699, 2218, 2687, 2767
HpySE526I	ACGT	7 cut(s)	792, 850, 1625, 1699, 2218, 2687, 2767
Hsp92I	GRCGYC	1 cut(s)	2551
HspAI	GCGC	1 cut(s)	1244
Kpn2I	TCCGGA	1 cut(s)	3286
Ksp22I	TGATCA	4 cut(s)	1218, 2676, 3097, 3145
KspAI	GTTAAC	2 cut(s)	2089, 2685
LguI	GCTCTTC	2 cut(s)	1938, 2861
LmnI	GCTCC	4 cut(s)	232, 1390, 2044, 3234
LweI	GCATC	6 cut(s)	130, 663, 2127, 2185, 2980, 3058
MaeI	CTAG	8 cut(s)	201, 282, 960, 1503, 1580, 2099, 3482, 3569
MaeII	ACGT	7 cut(s)	792, 850, 1625, 1699, 2218, 2687, 2767
MaeIII	GTNAC	4 cut(s)	339, 1202, 2746, 2887
MfeI	CAATTG	3 cut(s)	870, 1758, 2381
MflI	RGATCY	3 cut(s)	590, 728, 3459
MhlI	GDGCHC	2 cut(s)	8, 2228
MlsI	TGGCCA	1 cut(s)	1819
MluNI	TGGCCA	1 cut(s)	1819
MlyI	GAGTC	4 cut(s)	325, 1549, 1939, 2232
MmeI	TCCRAC	2 cut(s)	1353, 2697
Mox20I	TGGCCA	1 cut(s)	1819
Mph1103I	ATGCAT	1 cut(s)	145
MroI	TCCGGA	1 cut(s)	3286
MroXI	GAANNNNTTC	3 cut(s)	1038, 1999, 2444
MscI	TGGCCA	1 cut(s)	1819
Msp20I	TGGCCA	1 cut(s)	1819
MspA1I	CMGCKG	2 cut(s)	2557, 3154
MspCI	CTTAAG	3 cut(s)	1043, 2777, 3134
MspI	CCGG	2 cut(s)	1096, 3287
MspR9I	CCNGG	5 cut(s)	218, 231, 1358, 2414, 2900
MunI	CAATTG	3 cut(s)	870, 1758, 2381
Mva1269I	GAATGC	1 cut(s)	15
MvaI	CCWGG	5 cut(s)	218, 231, 1358, 2414, 2900
NdeI	CATATG	2 cut(s)	1147, 2188
NheI	GCTAGC	1 cut(s)	959
NlaIV	GGNNCC	7 cut(s)	228, 902, 3025, 3052, 3214, 3360, 3630
NmeAIII	GCCGAG	2 cut(s)	1563, 2388
NmuCI	GTSAC	1 cut(s)	1202
NsiI	ATGCAT	1 cut(s)	145
NspI	RCATGY	6 cut(s)	26, 374, 778, 1180, 2378, 2491
NspV	TTCGAA	1 cut(s)	3166
PaeI	GCATGC	1 cut(s)	26
PaeR7I	CTCGAG	1 cut(s)	307
PagI	TCATGA	2 cut(s)	1726, 2155
PciI	ACATGT	2 cut(s)	1176, 2374
PciSI	GCTCTTC	2 cut(s)	1938, 2861
PcsI	WCGNNNNNNNCGW	1 cut(s)	2623
PctI	GAATGC	1 cut(s)	15
PdmI	GAANNNNTTC	3 cut(s)	1038, 1999, 2444
PfeI	GAWTC	9 cut(s)	428, 616, 1102, 1800, 2183, 2545, 3395, 3494, 3599
PflMI	CCANNNNNTGG	1 cut(s)	2814
PfoI	TCCNGGA	1 cut(s)	1356
PleI	GAGTC	4 cut(s)	325, 1548, 1938, 2232
PpsI	GAGTC	4 cut(s)	325, 1548, 1938, 2232
Ppu21I	YACGTR	1 cut(s)	851
PscI	ACATGT	2 cut(s)	1176, 2374
Psp124BI	GAGCTC	1 cut(s)	2228
Psp1406I	AACGTT	2 cut(s)	1625, 2767
Psp6I	CCWGG	5 cut(s)	216, 229, 1356, 2412, 2898
PspFI	CCCAGC	1 cut(s)	2529
PspGI	CCWGG	5 cut(s)	216, 229, 1356, 2412, 2898
PspN4I	GGNNCC	7 cut(s)	228, 902, 3025, 3052, 3214, 3360, 3630
PspXI	VCTCGAGB	1 cut(s)	307
PstNI	CAGNNNCTG	2 cut(s)	593, 1280
PsuI	RGATCY	3 cut(s)	590, 728, 3459
PvuII	CAGCTG	1 cut(s)	3154
RsaI	GTAC	5 cut(s)	748, 779, 1119, 1527, 2036
RsaNI	GTAC	5 cut(s)	747, 778, 1118, 1526, 2035
SacI	GAGCTC	1 cut(s)	2228
SapI	GCTCTTC	2 cut(s)	1938, 2861
ScaI	AGTACT	2 cut(s)	748, 2036
SchI	GAGTC	4 cut(s)	325, 1549, 1939, 2232
ScrFI	CCNGG	5 cut(s)	218, 231, 1358, 2414, 2900
SduI	GDGCHC	2 cut(s)	8, 2228
SfaNI	GCATC	6 cut(s)	130, 663, 2127, 2185, 2980, 3058
SfcI	CTRYAG	1 cut(s)	922
Sfr274I	CTCGAG	1 cut(s)	307
SfuI	TTCGAA	1 cut(s)	3166
SinI	GGWCC	6 cut(s)	292, 314, 1711, 2093, 3358, 3628
SlaI	CTCGAG	1 cut(s)	307
SmlI	CTYRAG	7 cut(s)	307, 1043, 2777, 2939, 2981, 3134, 3521
SmoI	CTYRAG	7 cut(s)	307, 1043, 2777, 2939, 2981, 3134, 3521
SphI	GCATGC	1 cut(s)	26
SsiI	CCGC	3 cut(s)	2557, 2774, 3439
SspI	AATATT	1 cut(s)	810
SspMI	CTAG	8 cut(s)	201, 282, 960, 1503, 1580, 2099, 3482, 3569
SstI	GAGCTC	1 cut(s)	2228
StyD4I	CCNGG	5 cut(s)	216, 229, 1356, 2412, 2898
TaaI	ACNGT	7 cut(s)	751, 1265, 2286, 2668, 3304, 3422, 3535
TaiI	ACGT	7 cut(s)	795, 853, 1628, 1702, 2221, 2690, 2770
TatI	WGTACW	3 cut(s)	746, 777, 2034
TauI	GCSGC	2 cut(s)	2776, 3442
TfiI	GAWTC	9 cut(s)	428, 616, 1102, 1800, 2183, 2545, 3395, 3494, 3599
TscAI	CASTG	4 cut(s)	103, 689, 961, 999
TseFI	GTSAC	1 cut(s)	1202
Tsp45I	GTSAC	1 cut(s)	1202
TspGWI	ACGGA	1 cut(s)	2106
TspRI	CASTG	4 cut(s)	103, 689, 961, 999
Van91I	CCANNNNNTGG	1 cut(s)	2814
Vha464I	CTTAAG	3 cut(s)	1043, 2777, 3134
VpaK11BI	GGWCC	6 cut(s)	292, 314, 1711, 2093, 3358, 3628
XapI	RAATTY	9 cut(s)	91, 270, 971, 980, 1466, 2110, 3127, 3294, 3348
XbaI	TCTAGA	2 cut(s)	200, 2098
XceI	RCATGY	6 cut(s)	26, 374, 778, 1180, 2378, 2491
XcmI	CCANNNNNNNNNTGG	1 cut(s)	308
XhoI	CTCGAG	1 cut(s)	307
XmiI	GTMKAC	1 cut(s)	1181
XmnI	GAANNNNTTC	3 cut(s)	1038, 1999, 2444
XspI	CTAG	8 cut(s)	201, 282, 960, 1503, 1580, 2099, 3482, 3569
ZrmI	AGTACT	2 cut(s)	748, 2036
Zsp2I	ATGCAT	1 cut(s)	145

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.