Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0008336

Physical Location & Seq

Genomic Coordinates Forward (+)

29576176 .. 29588100

Gene Structure 11925 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1211400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2301 bp

ATGGATTTCGTGGAACTGGAGGCGATCGAGGGCCTCCGATGGCCCTGGAATTCATGGCCGCCGTCAAAATCCGAAAGCGCCGCCCTCGTCATCCCTCTCAGCATCATGTGCACGCCGCTGATGCAGCAGGCCGAGCTCCCGATACTTCCCTACGACCCCGTCACATGTCTCCGATGTGGCGCCGTTTTGAACCCCTACGCGCGCGTTGAATACCTTTCTCGCATCTGGTTCTGCTCCTTCTGCCACCACAAGAACTCCTTCCCTCACTCTTACTCCAACATCGGCGACACCAACCTCCCAGCCGAGCTTTTTCCGACTTACAGCGCCGTCGAGTACGCCAAAACCGCCCACGGAATTCCCAATTCCGGCTCGAATTCCCTGAATTGGGCAAATGGGTTGTCGTCATCGTCGTCCTTGTCGTCTATGGTTTCGTCGTCGGCGGCCTCGGTGTCGGATGGGGATTCGAGAGGGGTGAGGCCGGCGTTCGTGTTCGTTGTAGACGCTTGCACGCCCGGGGACGAGCTTGGAGTTCTGAAAAATGAGCTCTTGCTTGTTGTGGAGAAGCTTCCGGAGACTGCTTTGGTGGGTCTGTGCTCGAGGGCCGTGGTGTTTCACGGCGAAAGGGAGCTGTCATCGCTTCAGACCCAACGATTTCTAGGCATTAAGCGAACAAAGTATCAGCAACTCGGAAAGATGTCAGTTAATCAAAAGCAGGGCTTTTTGCTACCTGTTTCTGAATGTGAGTTCAACATCACAACTGCAATTGAAGAGATCCATTGTTCACTTCAAGTCAAGTCCGGTCATCGCCCACGAAGGTGTACAGGAGCAGCAATATTAGCTGCACTTGGACTTCTGGAAGGATGTTCAGGGAGCACGGGTTCCAGGATCATGGTCTTCACATCTGGACCTGCGACTTTAGGCCCAGGAATAATCGTCGATTCAGATCTTAGCAAGTCCATCAGAACTCACCGAGACCTCATCAATGGTCATGCACCTTACTATAGTAGATCTTCCAGCTTTTATAGGCAAGTGTTGCAGAGGTTAACTGATGCATCTATTGTTCTCGATATGTTTGCTTGTTCTCTTGATCAAACTGGAATTGCAGAGCTGAAGGTCCCTGTTGAGAAGTCAGGTGGTTTCATGTTGCTAGGTGAGTCATTTTCTTCAAATCAATTCAGAAAATGTCTGAGCCACATTTTTACTCGTGATGAAGGGGGATATTTGAAGATGTATTTCGATGCAACTATTGAGATAGTGACCACTAAAGATATTAAAATCTGTGGAGCCCTAGGTCCTTGTGTATCTCTTCGTGAAGTCAACGATTTAGTGAGTAGCAGTGAGATTGGGGAGGGTGGTACCTATATGTGGAAGTTGGGTACAATCACCAATAAAACATGCATTGCTTTTTTATTCCAAGTGAGTGATGAGCAGAAAGTTCAACCTGGGTCTGCATTTTTGATACAGTTCATAACACGATACCGTCATGGGAACATGAGAATCCGGAAAAGGGTAACAACTGCAGCAAGAAGATGGGTTGGAGGCCGTTCACCAGAAATTGCTGCTGGGTTTGATCAAGAGGCAGCAGCTTCAGTGATAGCCAGGCTTGCTATTCACCGAGCTGAGAATTGTTTCGCTCGAGATGTTATCAGATGGCTGGATGACACATTGATCCGCTTTGCTTCCAAGTTTGGAGACTATGCAGAGGAAGATCCAACTTCCTTCCGCTTGTCATCCAACTTCTCCCTCTTTCCTCAGTTCATGTATTACTTGAGGAGGTCGCAGTTTATTGATGTCTTCAACAATAGTCCTGATGAGACTGCTTTCTTCCGGCTGATGCTGAATCGCGAGGGGGTGGTGGGTTCTCTTATCATGATCCAACCCACGCTTTTCCAATACTCCTTTGATGGTCCACCCGTTCCAGTCCTCCTAGATGTCCGCTCCATCTCTCCAGATGTTATCATGCTCTTTGACTCATATTTTCACGTGGTTATTCATTACGGGTCCAAAGTTTCTCAGTGGAGGAAGCTTGGGTATGACAAGGACCCAAACCATGAGAATTTGAGGAAGCTGTTGGAAGCCCCGGAAATTGATGCAGAGCAGTTGATGGCTGACCGAGTTCCTGCACCGAAGTTTATTAAATGTGACCAGCATGGTAGCCAGGCAAGGTTTCTTCTTGCAAAGTTGAATCAATCTGTTACTCAGAATTCAACATACACTGACAGCTCGGATATTATTTTCACTGATGATCTGAGCTTGCAAGTATTTTTAGATCACTTGCAGGTCCTTGCAGTGCAAGGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001501 GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005798 GO:0005829 GO:0006810 GO:0006888 GO:0006900 GO:0006996 GO:0007275 GO:0008150 GO:0008270 GO:0009653 GO:0009790 GO:0009792 GO:0009887 GO:0009888 GO:0009987 GO:0012505 GO:0012506 GO:0012507 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0019898 GO:0022607 GO:0030117 GO:0030120 GO:0030127 GO:0030133 GO:0030134 GO:0030135 GO:0030658 GO:0030659 GO:0030660 GO:0030662 GO:0031090 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032991 GO:0033333 GO:0033334 GO:0033339 GO:0034622 GO:0035107 GO:0035138 GO:0035459 GO:0043009 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0046907 GO:0046914 GO:0048193 GO:0048194 GO:0048475 GO:0048513 GO:0048562 GO:0048568 GO:0048598 GO:0048701 GO:0048702 GO:0048704 GO:0048705 GO:0048706 GO:0048731 GO:0048736 GO:0048856 GO:0051179 GO:0051216 GO:0051234 GO:0051641 GO:0051649 GO:0051668 GO:0061024 GO:0061448 GO:0065003 GO:0070971 GO:0071840 GO:0090110 GO:0090114 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:1904888

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

Gelsolin Sec23_BS Sec23_helical Sec23_trunk zf-Sec23_Sec24

Protein Analysis

767

Amino Acids

85.14

Weight (kDa)

6.4

Isoelectric Point (pI)

49.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-Sec23_Sec24	PF04810	53 - 91	2.4e-12	Sec23/Sec24 zinc finger
Sec23_trunk	PF04811	160 - 400	1.5e-42	Sec23/Sec24 trunk domain
Sec23_BS	PF08033	412 - 512	2.4e-19	Sec23/Sec24 beta-sandwich domain
Sec23_helical	PF04815	524 - 621	5.8e-25	Sec23/Sec24 helical domain
Gelsolin	PF00626	638 - 724	1.1e-08	Gelsolin repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008336)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G43670
fragaria_vesca	FvH4_3g28910 FvH4_3g28910 FvH4_3g28910
malus_domestica	MD04G1211400.v1.1 MD12G1226200.v1.1
prunus_persica	Prupe.1G027900_v2.0.a1
pyrus_communis	pycom04g01860 pycom04g18730 pycom04g18740 pycom12g21060 pycom12g21090
rosa_chinensis	RchiOBHm_Chr5g0051941
rosa_laevigata	RLG00000034852
rosa_multiflora	Rmu_co8514153.1_g000001
rosa_roxburghii	Rroxscaffold_1G00027500
rosa_rugosa	Rorug05G0276200 Rorug05G0276300
rosa_samantha	Rh5AG348200 Rh5BG358800 Rh5CG384400 Rh5DG374200
rosa_wichuraiana	Rw5G032860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	916, 2269
Acc65I	GGTACC	1 cut(s)	1355
AccB1I	GGYRCC	2 cut(s)	179, 1355
AccBSI	CCGCTC	1 cut(s)	1938
AccI	GTMKAC	1 cut(s)	498
AccII	CGCG	4 cut(s)	200, 202, 204, 1845
AccIII	TCCGGA	2 cut(s)	568, 1500
AciI	CCGC	8 cut(s)	59, 81, 116, 345, 440, 1672, 1723, 1936
AclWI	GGATC	5 cut(s)	766, 893, 1663, 1703, 1867
AcoI	YGGCCR	1 cut(s)	56
AcsI	RAATTY	5 cut(s)	49, 354, 373, 2056, 2203
AcuI	CTGAAG	3 cut(s)	623, 1130, 1572
AcvI	CACGTG	1 cut(s)	1984
AcyI	GRCGYC	1 cut(s)	180
AfaI	GTAC	4 cut(s)	335, 820, 1357, 1378
AfiI	CCNNNNNNNGG	5 cut(s)	365, 384, 385, 1365, 1507
AflIII	ACRYGT	1 cut(s)	164
AjnI	CCWGG	6 cut(s)	44, 881, 922, 1441, 1598, 2157
AleI	CACNNNNGTG	1 cut(s)	814
AloI	GAACNNNNNNTCC	2 cut(s)	862, 894
Alw21I	GWGCWC	5 cut(s)	113, 138, 546, 596, 875
Alw26I	GTCTC	5 cut(s)	173, 566, 966, 1686, 1808
Alw44I	GTGCAC	1 cut(s)	109
AlwI	GGATC	5 cut(s)	766, 893, 1663, 1703, 1867
Ama87I	CYCGRG	3 cut(s)	512, 595, 1635
Aor13HI	TCCGGA	2 cut(s)	568, 1500
AoxI	GGCC	9 cut(s)	31, 41, 56, 129, 441, 476, 600, 919, 1540
ApaLI	GTGCAC	1 cut(s)	109
ApeKI	GCWGC	7 cut(s)	124, 827, 839, 1520, 1559, 1580, 1583
ApoI	RAATTY	5 cut(s)	49, 354, 373, 2056, 2203
ArsI	GACNNNNNNTTYG	2 cut(s)	383, 415
Asp700I	GAANNNNTTC	2 cut(s)	257, 1628
Asp718I	GGTACC	1 cut(s)	1355
AspA2I	CCTAGG	1 cut(s)	1288
AspLEI	GCGC	5 cut(s)	80, 182, 202, 204, 326
AsuC2I	CCSGG	3 cut(s)	513, 514, 2081
AsuHPI	GGTGA	6 cut(s)	484, 959, 1163, 1375, 1539, 1604
AvaI	CYCGRG	3 cut(s)	512, 595, 1635
AvaII	GGWCC	7 cut(s)	905, 1114, 1292, 1907, 2001, 2041, 2281
AvrII	CCTAGG	1 cut(s)	1288
BaeGI	GKGCMC	1 cut(s)	113
BanI	GGYRCC	2 cut(s)	179, 1355
BanII	GRGCYC	3 cut(s)	138, 546, 1288
BauI	CACGAG	1 cut(s)	1203
BbrPI	CACGTG	1 cut(s)	1984
BbsI	GAAGAC	2 cut(s)	886, 1786
Bbv12I	GWGCWC	5 cut(s)	113, 138, 546, 596, 875
BbvI	GCAGC	7 cut(s)	136, 826, 839, 1532, 1546, 1592, 1595
BccI	CCATC	8 cut(s)	33, 449, 965, 1524, 1644, 1898, 1949, 2098
BceAI	ACGGC	6 cut(s)	46, 167, 311, 587, 631, 1527
BcgI	CGANNNNNNTGC	2 cut(s)	1055, 1089
BciT130I	CCWGG	6 cut(s)	46, 883, 924, 1443, 1600, 2159
BclI	TGATCA	2 cut(s)	1087, 1570
BcnI	CCSGG	3 cut(s)	513, 514, 2081
BcoDI	GTCTC	5 cut(s)	173, 566, 966, 1686, 1808
BfaI	CTAG	4 cut(s)	656, 1148, 1289, 1928
BfmI	CTRYAG	2 cut(s)	1000, 1518
BfoI	RGCGCY	3 cut(s)	81, 183, 327
BfuAI	ACCTGC	2 cut(s)	916, 2269
BglII	AGATCT	2 cut(s)	943, 1007
BlnI	CCTAGG	1 cut(s)	1288
Bme1390I	CCNGG	9 cut(s)	46, 513, 514, 883, 924, 1443, 1600, 2081, 2159
Bme18I	GGWCC	7 cut(s)	905, 1114, 1292, 1907, 2001, 2041, 2281
BmeT110I	CYCGRG	3 cut(s)	512, 595, 1635
BmiI	GGNNCC	7 cut(s)	181, 880, 1116, 1285, 1357, 2002, 2043
BmrFI	CCNGG	9 cut(s)	46, 513, 514, 883, 924, 1443, 1600, 2081, 2159
BmsI	GCATC	8 cut(s)	111, 111, 231, 1039, 1061, 1228, 1824, 2080
BpiI	GAAGAC	2 cut(s)	886, 1786
BpmI	CTGGAG	2 cut(s)	38, 1932
BpuEI	CTTGAG	1 cut(s)	1789
BpuMI	CCSGG	3 cut(s)	513, 514, 2081
BsaAI	YACGTR	1 cut(s)	1984
BsaBI	GATNNNNATC	1 cut(s)	942
BsaHI	GRCGYC	1 cut(s)	180
BsaI	GGTCTC	1 cut(s)	966
BsaWI	WCCGGW	3 cut(s)	568, 797, 1500
BsaXI	ACNNNNNCTCC	6 cut(s)	239, 257, 269, 287, 862, 892
Bsc4I	CCNNNNNNNGG	5 cut(s)	365, 384, 385, 1365, 1507
Bse118I	RCCGGY	1 cut(s)	478
Bse1I	ACTGG	3 cut(s)	21, 1099, 1919
Bse3DI	GCAATG	1 cut(s)	1398
Bse8I	GATNNNNATC	1 cut(s)	942
BseAI	TCCGGA	2 cut(s)	568, 1500
BseBI	CCWGG	6 cut(s)	46, 883, 924, 1443, 1600, 2159
BseGI	GGATG	5 cut(s)	90, 460, 866, 1663, 1730
BseJI	GATNNNNATC	1 cut(s)	942
BseLI	CCNNNNNNNGG	5 cut(s)	365, 384, 385, 1365, 1507
BseMI	GCAATG	1 cut(s)	1398
BseMII	CTCAG	7 cut(s)	112, 1178, 1611, 1766, 2027, 2213, 2240
BseNI	ACTGG	3 cut(s)	21, 1099, 1919
BsePI	GCGCGC	1 cut(s)	200
BseRI	GAGGAG	1 cut(s)	1786
BseSI	GKGCMC	1 cut(s)	113
BseXI	GCAGC	7 cut(s)	136, 826, 839, 1532, 1546, 1592, 1595
BseYI	CCCAGC	2 cut(s)	298, 1562
BsgI	GTGCAG	2 cut(s)	825, 2106
Bsh1236I	CGCG	4 cut(s)	200, 202, 204, 1845
Bsh1285I	CGRYCG	1 cut(s)	27
BshFI	GGCC	9 cut(s)	33, 43, 58, 131, 443, 478, 602, 921, 1542
BshNI	GGYRCC	2 cut(s)	179, 1355
BsiEI	CGRYCG	1 cut(s)	27
BsiHKAI	GWGCWC	5 cut(s)	113, 138, 546, 596, 875
BsiHKCI	CYCGRG	3 cut(s)	512, 595, 1635
BsiSI	CCGG	8 cut(s)	366, 479, 513, 569, 798, 1501, 1828, 2081
BslFI	GGGAC	2 cut(s)	530, 1100
BslI	CCNNNNNNNGG	5 cut(s)	365, 384, 385, 1365, 1507
BsmAI	GTCTC	5 cut(s)	173, 566, 966, 1686, 1808
BsmFI	GGGAC	2 cut(s)	530, 1100
BsnI	GGCC	9 cut(s)	33, 43, 58, 131, 443, 478, 602, 921, 1542
Bso31I	GGTCTC	1 cut(s)	966
BsoBI	CYCGRG	3 cut(s)	512, 595, 1635
Bsp1286I	GDGCHC	6 cut(s)	113, 138, 546, 596, 875, 1288
Bsp13I	TCCGGA	2 cut(s)	568, 1500
Bsp1407I	TGTACA	1 cut(s)	818
Bsp68I	TCGCGA	1 cut(s)	1845
BspACI	CCGC	8 cut(s)	59, 81, 116, 345, 440, 1672, 1723, 1936
BspANI	GGCC	9 cut(s)	33, 43, 58, 131, 443, 478, 602, 921, 1542
BspCNI	CTCAG	7 cut(s)	111, 1179, 1612, 1765, 2026, 2212, 2241
BspEI	TCCGGA	2 cut(s)	568, 1500
BspFNI	CGCG	4 cut(s)	200, 202, 204, 1845
BspHI	TCATGA	1 cut(s)	1869
BspLI	GGNNCC	7 cut(s)	181, 880, 1116, 1285, 1357, 2002, 2043
BspMAI	CTGCAG	1 cut(s)	1522
BspMI	ACCTGC	2 cut(s)	916, 2269
BspPI	GGATC	5 cut(s)	766, 893, 1663, 1703, 1867
BspT107I	GGYRCC	2 cut(s)	179, 1355
BspTNI	GGTCTC	1 cut(s)	966
BsrBI	CCGCTC	1 cut(s)	1938
BsrDI	GCAATG	1 cut(s)	1398
BsrFI	RCCGGY	1 cut(s)	478
BsrGI	TGTACA	1 cut(s)	818
BsrI	ACTGG	3 cut(s)	21, 1099, 1919
BssAI	RCCGGY	1 cut(s)	478
BssHII	GCGCGC	1 cut(s)	200
BssNI	GRCGYC	1 cut(s)	180
BssSI	CACGAG	1 cut(s)	1203
BssT1I	CCWWGG	1 cut(s)	1288
Bst2BI	CACGAG	1 cut(s)	1203
Bst2UI	CCWGG	6 cut(s)	46, 883, 924, 1443, 1600, 2159
Bst4CI	ACNGT	2 cut(s)	1464, 1481
Bst6I	CTCTTC	2 cut(s)	762, 1311
BstACI	GRCGYC	1 cut(s)	180
BstAPI	GCANNNNNTGC	2 cut(s)	108, 1033
BstAUI	TGTACA	1 cut(s)	818
BstBAI	YACGTR	1 cut(s)	1984
BstC8I	GCNNGC	8 cut(s)	113, 129, 202, 480, 505, 509, 1605, 2255
BstDEI	CTNAG	8 cut(s)	98, 947, 1187, 1620, 1752, 2013, 2199, 2249
BstDSI	CCRYGG	2 cut(s)	349, 603
BstF5I	GGATG	5 cut(s)	90, 460, 866, 1663, 1730
BstFNI	CGCG	4 cut(s)	200, 202, 204, 1845
BstH2I	RGCGCY	3 cut(s)	81, 183, 327
BstHHI	GCGC	5 cut(s)	80, 182, 202, 204, 326
BstMAI	GTCTC	5 cut(s)	173, 566, 966, 1686, 1808
BstMCI	CGRYCG	1 cut(s)	27
BstNI	CCWGG	6 cut(s)	46, 883, 924, 1443, 1600, 2159
BstNSI	RCATGY	2 cut(s)	168, 1398
BstSCI	CCNGG	9 cut(s)	44, 511, 512, 881, 922, 1441, 1598, 2079, 2157
BstSFI	CTRYAG	2 cut(s)	1000, 1518
BstSLI	GKGCMC	1 cut(s)	113
BstUI	CGCG	4 cut(s)	200, 202, 204, 1845
BstV1I	GCAGC	7 cut(s)	136, 826, 839, 1532, 1546, 1592, 1595
BstV2I	GAAGAC	2 cut(s)	886, 1786
BstX2I	RGATCY	4 cut(s)	771, 943, 1007, 1708
BstXI	CCANNNNNNTGG	1 cut(s)	889
BstYI	RGATCY	4 cut(s)	771, 943, 1007, 1708
BsuRI	GGCC	9 cut(s)	33, 43, 58, 131, 443, 478, 602, 921, 1542
BtgI	CCRYGG	2 cut(s)	349, 603
BtgZI	GCGATG	2 cut(s)	618, 788
BtsCI	GGATG	5 cut(s)	90, 460, 866, 1663, 1730
BtsI	GCAGTG	2 cut(s)	1342, 2295
BtsIMutI	CAGTG	6 cut(s)	1342, 1596, 2021, 2214, 2238, 2295
BtuMI	TCGCGA	1 cut(s)	1845
BveI	ACCTGC	2 cut(s)	916, 2269
Cac8I	GCNNGC	8 cut(s)	113, 129, 202, 480, 505, 509, 1605, 2255
CciI	TCATGA	1 cut(s)	1869
CfoI	GCGC	5 cut(s)	80, 182, 202, 204, 326
Cfr10I	RCCGGY	1 cut(s)	478
Cfr9I	CCCGGG	1 cut(s)	512
CseI	GACGC	1 cut(s)	509
Csp6I	GTAC	4 cut(s)	334, 819, 1356, 1377
CviQI	GTAC	4 cut(s)	334, 819, 1356, 1377
DdeI	CTNAG	8 cut(s)	98, 947, 1187, 1620, 1752, 2013, 2199, 2249
DinI	GGCGCC	1 cut(s)	181
EaeI	YGGCCR	1 cut(s)	56
Eam1104I	CTCTTC	2 cut(s)	762, 1311
EarI	CTCTTC	2 cut(s)	762, 1311
Ecl136II	GAGCTC	2 cut(s)	136, 544
Eco130I	CCWWGG	1 cut(s)	1288
Eco24I	GRGCYC	3 cut(s)	138, 546, 1288
Eco31I	GGTCTC	1 cut(s)	966
Eco47I	GGWCC	7 cut(s)	905, 1114, 1292, 1907, 2001, 2041, 2281
Eco53kI	GAGCTC	2 cut(s)	136, 544
Eco57I	CTGAAG	3 cut(s)	623, 1130, 1572
Eco72I	CACGTG	1 cut(s)	1984
Eco88I	CYCGRG	3 cut(s)	512, 595, 1635
EcoICRI	GAGCTC	2 cut(s)	136, 544
EcoO109I	RGGNCCY	5 cut(s)	31, 1114, 1292, 2041, 2281
EcoRI	GAATTC	4 cut(s)	49, 354, 373, 2203
EcoRII	CCWGG	6 cut(s)	44, 881, 922, 1441, 1598, 2157
EcoT14I	CCWWGG	1 cut(s)	1288
EcoT22I	ATGCAT	2 cut(s)	1054, 1400
EcoT38I	GRGCYC	3 cut(s)	138, 546, 1288
EgeI	GGCGCC	1 cut(s)	181
EheI	GGCGCC	1 cut(s)	181
ErhI	CCWWGG	1 cut(s)	1288
FalI	AAGNNNNNCTT	4 cut(s)	242, 274, 701, 733
FaqI	GGGAC	2 cut(s)	530, 1100
FbaI	TGATCA	2 cut(s)	1087, 1570
FblI	GTMKAC	1 cut(s)	498
FokI	GGATG	5 cut(s)	77, 467, 873, 1670, 1717
FriOI	GRGCYC	3 cut(s)	138, 546, 1288
FspBI	CTAG	4 cut(s)	656, 1148, 1289, 1928
GlaI	GCGC	5 cut(s)	79, 181, 201, 203, 325
GsaI	CCCAGC	2 cut(s)	302, 1566
GsuI	CTGGAG	2 cut(s)	38, 1932
HaeII	RGCGCY	3 cut(s)	81, 183, 327
HaeIII	GGCC	9 cut(s)	33, 43, 58, 131, 443, 478, 602, 921, 1542
HapII	CCGG	8 cut(s)	366, 479, 513, 569, 798, 1501, 1828, 2081
HgaI	GACGC	1 cut(s)	509
HhaI	GCGC	5 cut(s)	80, 182, 202, 204, 326
Hin1I	GRCGYC	1 cut(s)	180
Hin6I	GCGC	5 cut(s)	78, 180, 200, 202, 324
HinP1I	GCGC	5 cut(s)	78, 180, 200, 202, 324
HincII	GTYRAC	2 cut(s)	1044, 1318
HindII	GTYRAC	2 cut(s)	1044, 1318
HindIII	AAGCTT	2 cut(s)	563, 2024
HinfI	GANTC	7 cut(s)	461, 938, 1154, 1497, 1840, 1970, 2185
HpaI	GTTAAC	1 cut(s)	1044
HpaII	CCGG	8 cut(s)	366, 479, 513, 569, 798, 1501, 1828, 2081
HphI	GGTGA	6 cut(s)	484, 959, 1163, 1375, 1539, 1604
Hpy166II	GTNNAC	8 cut(s)	111, 499, 782, 819, 1044, 1318, 1547, 1910
Hpy8I	GTNNAC	8 cut(s)	111, 499, 782, 819, 1044, 1318, 1547, 1910
Hpy99I	CGWCG	5 cut(s)	332, 412, 436, 439, 938
HpyAV	CCTTC	7 cut(s)	247, 268, 807, 851, 1105, 1205, 1729
HpyCH4III	ACNGT	2 cut(s)	1464, 1481
HpyCH4IV	ACGT	1 cut(s)	1983
HpyF3I	CTNAG	8 cut(s)	98, 947, 1187, 1620, 1752, 2013, 2199, 2249
HpySE526I	ACGT	1 cut(s)	1983
Hsp92I	GRCGYC	1 cut(s)	180
HspAI	GCGC	5 cut(s)	78, 180, 200, 202, 324
KasI	GGCGCC	1 cut(s)	179
Kpn2I	TCCGGA	2 cut(s)	568, 1500
KpnI	GGTACC	1 cut(s)	1359
KroI	GCCGGC	1 cut(s)	478
KroNI	GCCGGC	1 cut(s)	480
Ksp22I	TGATCA	2 cut(s)	1087, 1570
KspAI	GTTAAC	1 cut(s)	1044
LmnI	GCTCC	7 cut(s)	141, 239, 625, 824, 870, 1283, 1943
Lsp1109I	GCAGC	7 cut(s)	136, 826, 839, 1532, 1546, 1592, 1595
LweI	GCATC	8 cut(s)	111, 111, 231, 1039, 1061, 1228, 1824, 2080
MaeI	CTAG	4 cut(s)	656, 1148, 1289, 1928
MaeII	ACGT	1 cut(s)	1983
MaeIII	GTNAC	5 cut(s)	160, 1255, 1510, 2141, 2194
MauBI	CGCGCGCG	1 cut(s)	200
MbiI	CCGCTC	1 cut(s)	1938
MfeI	CAATTG	1 cut(s)	762
MflI	RGATCY	4 cut(s)	771, 943, 1007, 1708
MhlI	GDGCHC	6 cut(s)	113, 138, 546, 596, 875, 1288
Mly113I	GGCGCC	1 cut(s)	180
MlyI	GAGTC	2 cut(s)	1163, 1964
MmeI	TCCRAC	8 cut(s)	300, 338, 432, 1516, 1736, 1758, 1900, 2052
Mph1103I	ATGCAT	2 cut(s)	1054, 1400
MroI	TCCGGA	2 cut(s)	568, 1500
MroNI	GCCGGC	1 cut(s)	478
MroXI	GAANNNNTTC	2 cut(s)	257, 1628
MseI	TTAA	5 cut(s)	663, 702, 1043, 1272, 2136
MslI	CAYNNNNRTG	1 cut(s)	814
MspA1I	CMGCKG	1 cut(s)	118
MspI	CCGG	8 cut(s)	366, 479, 513, 569, 798, 1501, 1828, 2081
MspR9I	CCNGG	9 cut(s)	46, 513, 514, 883, 924, 1443, 1600, 2081, 2159
MunI	CAATTG	1 cut(s)	762
MvaI	CCWGG	6 cut(s)	46, 883, 924, 1443, 1600, 2159
MvnI	CGCG	4 cut(s)	200, 202, 204, 1845
NaeI	GCCGGC	1 cut(s)	480
NarI	GGCGCC	1 cut(s)	180
NciI	CCSGG	3 cut(s)	513, 514, 2081
NgoMIV	GCCGGC	1 cut(s)	478
NlaIV	GGNNCC	7 cut(s)	181, 880, 1116, 1285, 1357, 2002, 2043
NmeAIII	GCCGAG	2 cut(s)	157, 328
NmuCI	GTSAC	3 cut(s)	160, 1255, 2141
NruI	TCGCGA	1 cut(s)	1845
NsiI	ATGCAT	2 cut(s)	1054, 1400
NspI	RCATGY	2 cut(s)	168, 1398
OliI	CACNNNNGTG	1 cut(s)	814
PaeR7I	CTCGAG	2 cut(s)	595, 1635
PagI	TCATGA	1 cut(s)	1869
PauI	GCGCGC	1 cut(s)	200
PciI	ACATGT	1 cut(s)	164
PcsI	WCGNNNNNNNCGW	2 cut(s)	407, 416
PdiI	GCCGGC	1 cut(s)	480
PdmI	GAANNNNTTC	2 cut(s)	257, 1628
PfeI	GAWTC	5 cut(s)	461, 938, 1497, 1840, 2185
PflFI	GACNNNGTC	1 cut(s)	158
PfoI	TCCNGGA	1 cut(s)	881
Ple19I	CGATCG	1 cut(s)	27
PleI	GAGTC	2 cut(s)	1162, 1964
PluTI	GGCGCC	1 cut(s)	183
PmaCI	CACGTG	1 cut(s)	1984
PmlI	CACGTG	1 cut(s)	1984
PpsI	GAGTC	2 cut(s)	1162, 1964
Ppu21I	YACGTR	1 cut(s)	1984
PpuMI	RGGWCCY	4 cut(s)	1114, 1292, 2041, 2281
PscI	ACATGT	1 cut(s)	164
Psp124BI	GAGCTC	2 cut(s)	138, 546
Psp5II	RGGWCCY	4 cut(s)	1114, 1292, 2041, 2281
Psp6I	CCWGG	6 cut(s)	44, 881, 922, 1441, 1598, 2157
PspCI	CACGTG	1 cut(s)	1984
PspFI	CCCAGC	2 cut(s)	298, 1562
PspGI	CCWGG	6 cut(s)	44, 881, 922, 1441, 1598, 2157
PspN4I	GGNNCC	7 cut(s)	181, 880, 1116, 1285, 1357, 2002, 2043
PspPPI	RGGWCCY	4 cut(s)	1114, 1292, 2041, 2281
PspXI	VCTCGAGB	1 cut(s)	595
PstI	CTGCAG	1 cut(s)	1522
PsuI	RGATCY	4 cut(s)	771, 943, 1007, 1708
PsyI	GACNNNGTC	1 cut(s)	158
PteI	GCGCGC	1 cut(s)	200
PvuI	CGATCG	1 cut(s)	27
RruI	TCGCGA	1 cut(s)	1845
RsaI	GTAC	4 cut(s)	335, 820, 1357, 1378
RsaNI	GTAC	4 cut(s)	334, 819, 1356, 1377
RseI	CAYNNNNRTG	1 cut(s)	814
SacI	GAGCTC	2 cut(s)	138, 546
SaqAI	TTAA	5 cut(s)	663, 702, 1043, 1272, 2136
SchI	GAGTC	2 cut(s)	1163, 1964
ScrFI	CCNGG	9 cut(s)	46, 513, 514, 883, 924, 1443, 1600, 2081, 2159
SduI	GDGCHC	6 cut(s)	113, 138, 546, 596, 875, 1288
SfaNI	GCATC	8 cut(s)	111, 111, 231, 1039, 1061, 1228, 1824, 2080
SfcI	CTRYAG	2 cut(s)	1000, 1518
SfoI	GGCGCC	1 cut(s)	181
Sfr274I	CTCGAG	2 cut(s)	595, 1635
SinI	GGWCC	7 cut(s)	905, 1114, 1292, 1907, 2001, 2041, 2281
SlaI	CTCGAG	2 cut(s)	595, 1635
SmaI	CCCGGG	1 cut(s)	514
SmiMI	CAYNNNNRTG	1 cut(s)	814
SmlI	CTYRAG	3 cut(s)	595, 1635, 1768
SmoI	CTYRAG	3 cut(s)	595, 1635, 1768
SsiI	CCGC	8 cut(s)	59, 81, 116, 345, 440, 1672, 1723, 1936
SspDI	GGCGCC	1 cut(s)	179
SspI	AATATT	1 cut(s)	834
SspMI	CTAG	4 cut(s)	656, 1148, 1289, 1928
SstI	GAGCTC	2 cut(s)	138, 546
StyD4I	CCNGG	9 cut(s)	44, 511, 512, 881, 922, 1441, 1598, 2079, 2157
StyI	CCWWGG	1 cut(s)	1288
TaaI	ACNGT	2 cut(s)	1464, 1481
TaiI	ACGT	1 cut(s)	1986
TaqI	TCGA	9 cut(s)	27, 330, 371, 464, 596, 936, 1065, 1236, 1636
TaqII	GACCGA	1 cut(s)	2127
TatI	WGTACW	1 cut(s)	818
TauI	GCSGC	4 cut(s)	61, 83, 118, 443
TfiI	GAWTC	5 cut(s)	461, 938, 1497, 1840, 2185
Tru1I	TTAA	5 cut(s)	663, 702, 1043, 1272, 2136
Tru9I	TTAA	5 cut(s)	663, 702, 1043, 1272, 2136
TscAI	CASTG	6 cut(s)	1342, 1596, 2021, 2221, 2245, 2295
TseFI	GTSAC	3 cut(s)	160, 1255, 2141
TseI	GCWGC	7 cut(s)	124, 827, 839, 1520, 1559, 1580, 1583
Tsp45I	GTSAC	3 cut(s)	160, 1255, 2141
TspDTI	ATGAA	6 cut(s)	42, 1129, 1224, 1456, 1747, 1982
TspGWI	ACGGA	1 cut(s)	366
TspMI	CCCGGG	1 cut(s)	512
TspRI	CASTG	6 cut(s)	1342, 1596, 2021, 2221, 2245, 2295
Tth111I	GACNNNGTC	1 cut(s)	158
VneI	GTGCAC	1 cut(s)	109
VpaK11BI	GGWCC	7 cut(s)	905, 1114, 1292, 1907, 2001, 2041, 2281
XapI	RAATTY	5 cut(s)	49, 354, 373, 2056, 2203
XceI	RCATGY	2 cut(s)	168, 1398
XhoI	CTCGAG	2 cut(s)	595, 1635
XmaI	CCCGGG	1 cut(s)	512
XmaJI	CCTAGG	1 cut(s)	1288
XmiI	GTMKAC	1 cut(s)	498
XmnI	GAANNNNTTC	2 cut(s)	257, 1628
XspI	CTAG	4 cut(s)	656, 1148, 1289, 1928
Zsp2I	ATGCAT	2 cut(s)	1054, 1400

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.