Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_co8514153.1

Orthogroup ID

OG0008336

Physical Location & Seq

Genomic Coordinates Reverse (-)

834 .. 4013

Gene Structure 3180 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rmu_co8514153.1_g000001.1.cds

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2334 bp

atggatttcgtggaactggaagccatagaaggcctccgatggtcctggaactcgtggccgtcttccaaagccgaccaagccgctctcgtcatccccctaagcgtcatgtgcacgccgctgatgcaacaagccgagctcccactcctcccctacgaccccgtcacctgctcccaatgcggcgccgttttgaacccctacgcgcgcgtcgactacacctcccgcatctggttctgctccttgtgcctccacaagaaccccttccctcactcctactccaacatcggcgacaccaatctccccgccgagctcttccccacttacagcgccgtcgagtacgcccgaaccgccccgactcccgccccggcccacaattggcccaacggcggcctctcgtcctcgtcctcgctgtcctcgatggtgtcgtcgtcgccaaactccgggtccgacgattcgcgagggatccgacccgcgtttgtgttggtcgtcgacgtggccacgccccgggatgagctggcggctctgaagaacgagctgctgcttgtgctggagcaactgccggagactgctctggtgggtttagtcacgtttgattccatggtgcgtgttcatgatctcgagttttccgagtgctccagagtcgttgtgcttcacggagaccggcagctctcatcgcttcagattcaacaatttctgggcattaataggcctaggcagcagcaactcggaaaaatgcctttttcccaaaagcaagggttcttgctaccagtatctgagtgtgagttcaacatttcaactgcaattgaagaaatccagtcttcggcagtcaagcctggtcatcgcccaggaaggtcaacaggagcagcaatttcagccgcacttggacttttggaaggatgcttggtgagcactggctccagaatcatggtcttcacatctggacctgcaactgtaggcccaggaattattgtagattctgatcttggttattccatcagaactcaccgagatctcatcaatggtcatgccccttactatagtagatcttgcagcttctacagacaagtgtcacagaggttaactgatgcatctattgtccttgatttgtttgcttgttctcttgatcaagctggctctgcggaacttaaggatcctgttgagagatcgggtggtttcatgatgctaggtgagtcctttgagtcgaatcagttcagaaaatgtttgcggcacatttttactcgtgatgaagaggggtatttgaagatgtattttgatgcaactattgagatagtgaccactaaagatgttaaaatctgtggagccctaggtccttgtgtgtctcttcggaaagttaacaatttagtgagtagcactgagattggtgagggtggtacctatatatggaagttaggtactattaccaataaaacatgcatcgcctttttctttgaagttagtgatgagcagaaagttcaacttggctcggcattctttatacagttcataacacgataccgacatgggaacatgggaatccggaaacgggtgacaactgctgcaagaagatgggttggtaaccgttcaccagaaattgctgctgggttcgatcaagaaacagctgcttctgtgatagccaggcttgctattcaccgagctgagaattgttttgctcgagatgttattagatggctggatgacacgttgatccattttgcttcaaaatttggagactatgtggaggaagatccatcttctttccgcttggcatccaacttctctctttttccccaattcatgtattacttgaggaggtctcagtttattgatgtctttaatagtagccctgatgagacagctttctttcggctgatgctgaaccgtgagggcgtggtgggttctcttatcatgatccagcctacacttctccaatattcctttgacggtccacccattccagtcctcctggatgttcgctctatctctccagatgttatcttgctctttgattcttactttcatgtggttattcactatgggtccaagattgctcaatggaggaagcttggttatgataaggatccaaatcatgagaatttgaggaagctgttggaagccccagaaattgatgcacagcagttggtggctgaccgtgttcctgcaccgaagctcattaaatgtgaccagcatggtagccaggcaaggtttcttcttgcaaagttgaatccatcggttacccagaattcaacatacacggatggctcggatattatcttgactgatgatttgagcttgcaagtatttttagatcacttgcagggcctggcagtgcaaggctga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001501 GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005798 GO:0005829 GO:0006810 GO:0006888 GO:0006900 GO:0006996 GO:0007275 GO:0008150 GO:0008270 GO:0009653 GO:0009790 GO:0009792 GO:0009887 GO:0009888 GO:0009987 GO:0012505 GO:0012506 GO:0012507 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0019898 GO:0022607 GO:0030117 GO:0030120 GO:0030127 GO:0030133 GO:0030134 GO:0030135 GO:0030658 GO:0030659 GO:0030660 GO:0030662 GO:0031090 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032991 GO:0033333 GO:0033334 GO:0033339 GO:0034622 GO:0035107 GO:0035138 GO:0035459 GO:0043009 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0046907 GO:0046914 GO:0048193 GO:0048194 GO:0048475 GO:0048513 GO:0048562 GO:0048568 GO:0048598 GO:0048701 GO:0048702 GO:0048704 GO:0048705 GO:0048706 GO:0048731 GO:0048736 GO:0048856 GO:0051179 GO:0051216 GO:0051234 GO:0051641 GO:0051649 GO:0051668 GO:0061024 GO:0061448 GO:0065003 GO:0070971 GO:0071840 GO:0090110 GO:0090114 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:1904888

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

Gelsolin Sec23_BS Sec23_helical Sec23_trunk zf-Sec23_Sec24

Protein Analysis

777

Amino Acids

86.26

Weight (kDa)

5.77

Isoelectric Point (pI)

50.5

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008336)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G43670
fragaria_vesca	FvH4_3g28910 FvH4_3g28910 FvH4_3g28910
malus_domestica	MD04G1211400.v1.1 MD12G1226200.v1.1
prunus_persica	Prupe.1G027900_v2.0.a1
pyrus_communis	pycom04g01860 pycom04g18730 pycom04g18740 pycom12g21060 pycom12g21090
rosa_chinensis	RchiOBHm_Chr5g0051941
rosa_laevigata	RLG00000034852
rosa_multiflora	Rmu_co8514153.1_g000001
rosa_roxburghii	Rroxscaffold_1G00027500
rosa_rugosa	Rorug05G0276200 Rorug05G0276300
rosa_samantha	Rh5AG348200 Rh5BG358800 Rh5CG384400 Rh5DG374200
rosa_wichuraiana	Rw5G032860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	173
Acc36I	ACCTGC	2 cut(s)	173, 949
Acc65I	GGTACC	1 cut(s)	1388
AccB1I	GGYRCC	2 cut(s)	179, 1388
AccBSI	CCGCTC	1 cut(s)	83
AccI	GTMKAC	2 cut(s)	207, 486
AccII	CGCG	5 cut(s)	200, 202, 204, 454, 470
AccIII	TCCGGA	1 cut(s)	1533
AclWI	GGATC	9 cut(s)	454, 467, 1142, 1155, 1696, 1736, 1900, 2069, 2082
AcoI	YGGCCR	2 cut(s)	56, 492
AcsI	RAATTY	3 cut(s)	1718, 2089, 2236
AcuI	CTGAAG	2 cut(s)	542, 659
AcyI	GRCGYC	1 cut(s)	180
AfaI	GTAC	3 cut(s)	335, 1390, 1411
AfiI	CCNNNNNNNGG	8 cut(s)	225, 372, 383, 437, 501, 817, 1398, 1540
AflII	CTTAAG	1 cut(s)	1142
AflIII	ACRYGT	1 cut(s)	1695
AjiI	CACGTC	1 cut(s)	490
AjnI	CCWGG	8 cut(s)	44, 829, 841, 955, 1631, 1959, 2190, 2316
Alw21I	GWGCWC	5 cut(s)	113, 138, 309, 632, 908
Alw26I	GTCTC	6 cut(s)	554, 648, 1341, 1719, 1815, 1841
Alw44I	GTGCAC	1 cut(s)	109
AlwI	GGATC	9 cut(s)	454, 467, 1142, 1155, 1696, 1736, 1900, 2069, 2082
AlwNI	CAGNNNCTG	2 cut(s)	770, 2317
Ama87I	CYCGRG	3 cut(s)	501, 614, 1668
Aor13HI	TCCGGA	1 cut(s)	1533
AoxI	GGCC	9 cut(s)	31, 56, 363, 374, 385, 492, 704, 952, 2314
ApaLI	GTGCAC	1 cut(s)	109
ApoI	RAATTY	3 cut(s)	1718, 2089, 2236
ArsI	GACNNNNNNTTYG	4 cut(s)	425, 457, 2263, 2295
AseI	ATTAAT	1 cut(s)	699
Asp700I	GAANNNNTTC	2 cut(s)	257, 1205
Asp718I	GGTACC	1 cut(s)	1388
AspA2I	CCTAGG	2 cut(s)	707, 1321
AspLEI	GCGC	4 cut(s)	182, 202, 204, 326
AsuC2I	CCSGG	4 cut(s)	362, 439, 502, 503
AsuHPI	GGTGA	8 cut(s)	154, 913, 992, 1196, 1391, 1555, 1572, 1637
AvaI	CYCGRG	3 cut(s)	501, 614, 1668
AvaII	GGWCC	6 cut(s)	42, 441, 938, 1325, 1940, 2034
AvrII	CCTAGG	2 cut(s)	707, 1321
BaeGI	GKGCMC	1 cut(s)	113
BalI	TGGCCA	1 cut(s)	494
BamHI	GGATCC	3 cut(s)	459, 1147, 2074
BanI	GGYRCC	2 cut(s)	179, 1388
BanII	GRGCYC	3 cut(s)	138, 309, 1321
BauI	CACGAG	2 cut(s)	52, 1236
BbsI	GAAGAC	3 cut(s)	54, 807, 919
Bbv12I	GWGCWC	5 cut(s)	113, 138, 309, 632, 908
BccI	CCATC	8 cut(s)	33, 409, 998, 1557, 1677, 1753, 2230, 2246
BceAI	ACGGC	4 cut(s)	43, 167, 311, 397
BciT130I	CCWGG	8 cut(s)	46, 831, 843, 957, 1633, 1961, 2192, 2318
BclI	TGATCA	1 cut(s)	1120
BcnI	CCSGG	4 cut(s)	362, 439, 502, 503
BcoDI	GTCTC	6 cut(s)	554, 648, 1341, 1719, 1815, 1841
BfaI	CTAG	3 cut(s)	708, 1181, 1322
BfmI	CTRYAG	3 cut(s)	948, 1033, 1054
BfoI	RGCGCY	2 cut(s)	183, 327
BfrI	CTTAAG	1 cut(s)	1142
BfuAI	ACCTGC	2 cut(s)	173, 949
BglII	AGATCT	2 cut(s)	1006, 1040
BlnI	CCTAGG	2 cut(s)	707, 1321
Bme18I	GGWCC	6 cut(s)	42, 441, 938, 1325, 1940, 2034
BmeT110I	CYCGRG	3 cut(s)	501, 614, 1668
BmgBI	CACGTC	1 cut(s)	490
BmiI	GGNNCC	9 cut(s)	181, 442, 461, 913, 1149, 1318, 1390, 2035, 2076
BoxI	GACNNNNGTC	1 cut(s)	1063
BpiI	GAAGAC	3 cut(s)	54, 807, 919
BplI	GAGNNNNNCTC	2 cut(s)	1795, 1827
BpmI	CTGGAG	4 cut(s)	566, 616, 898, 1965
Bpu10I	CCTNAGC	1 cut(s)	98
BpuEI	CTTGAG	1 cut(s)	1822
BpuMI	CCSGG	4 cut(s)	362, 439, 502, 503
BsaBI	GATNNNNATC	3 cut(s)	975, 2073, 2079
BsaHI	GRCGYC	1 cut(s)	180
BsaI	GGTCTC	2 cut(s)	648, 1815
BsaJI	CCNNGG	8 cut(s)	360, 500, 501, 594, 707, 841, 955, 1321
BsaWI	WCCGGW	1 cut(s)	1533
BsaXI	ACNNNNNCTCC	6 cut(s)	200, 230, 257, 287, 1716, 1746
Bsc4I	CCNNNNNNNGG	8 cut(s)	225, 372, 383, 437, 501, 817, 1398, 1540
Bse118I	RCCGGY	1 cut(s)	657
Bse1I	ACTGG	5 cut(s)	21, 764, 811, 913, 1952
Bse8I	GATNNNNATC	3 cut(s)	975, 2073, 2079
BseAI	TCCGGA	1 cut(s)	1533
BseBI	CCWGG	8 cut(s)	46, 831, 843, 957, 1633, 1961, 2192, 2318
BseDI	CCNNGG	8 cut(s)	360, 500, 501, 594, 707, 841, 955, 1321
BseGI	GGATG	7 cut(s)	90, 511, 899, 1696, 1763, 1969, 2257
BseJI	GATNNNNATC	3 cut(s)	975, 2073, 2079
BseLI	CCNNNNNNNGG	8 cut(s)	225, 372, 383, 437, 501, 817, 1398, 1540
BseMII	CTCAG	4 cut(s)	762, 1362, 1644, 1826
BseNI	ACTGG	5 cut(s)	21, 764, 811, 913, 1952
BsePI	GCGCGC	1 cut(s)	200
BseRI	GAGGAG	2 cut(s)	134, 1819
BseSI	GKGCMC	1 cut(s)	113
BseYI	CCCAGC	1 cut(s)	1595
BsgI	GTGCAG	1 cut(s)	2139
Bsh1236I	CGCG	5 cut(s)	200, 202, 204, 454, 470
BshFI	GGCC	9 cut(s)	33, 58, 365, 376, 387, 494, 706, 954, 2316
BshNI	GGYRCC	2 cut(s)	179, 1388
BsiHKAI	GWGCWC	5 cut(s)	113, 138, 309, 632, 908
BsiHKCI	CYCGRG	3 cut(s)	501, 614, 1668
BsiSI	CCGG	6 cut(s)	362, 438, 502, 557, 658, 1534
BslI	CCNNNNNNNGG	8 cut(s)	225, 372, 383, 437, 501, 817, 1398, 1540
BsmAI	GTCTC	6 cut(s)	554, 648, 1341, 1719, 1815, 1841
BsmI	GAATGC	1 cut(s)	1484
BsnI	GGCC	9 cut(s)	33, 58, 365, 376, 387, 494, 706, 954, 2316
Bso31I	GGTCTC	2 cut(s)	648, 1815
BsoBI	CYCGRG	3 cut(s)	501, 614, 1668
Bsp1286I	GDGCHC	6 cut(s)	113, 138, 309, 632, 908, 1321
Bsp13I	TCCGGA	1 cut(s)	1533
Bsp19I	CCATGG	1 cut(s)	594
Bsp68I	TCGCGA	1 cut(s)	454
BspANI	GGCC	9 cut(s)	33, 58, 365, 376, 387, 494, 706, 954, 2316
BspCNI	CTCAG	4 cut(s)	763, 1363, 1645, 1825
BspEI	TCCGGA	1 cut(s)	1533
BspFNI	CGCG	5 cut(s)	200, 202, 204, 454, 470
BspHI	TCATGA	4 cut(s)	607, 1173, 1902, 2083
BspLI	GGNNCC	9 cut(s)	181, 442, 461, 913, 1149, 1318, 1390, 2035, 2076
BspMI	ACCTGC	2 cut(s)	173, 949
BspPI	GGATC	9 cut(s)	454, 467, 1142, 1155, 1696, 1736, 1900, 2069, 2082
BspQI	GCTCTTC	1 cut(s)	314
BspT107I	GGYRCC	2 cut(s)	179, 1388
BspTI	CTTAAG	1 cut(s)	1142
BspTNI	GGTCTC	2 cut(s)	648, 1815
BsrBI	CCGCTC	1 cut(s)	83
BsrFI	RCCGGY	1 cut(s)	657
BsrI	ACTGG	5 cut(s)	21, 764, 811, 913, 1952
BssAI	RCCGGY	1 cut(s)	657
BssECI	CCNNGG	8 cut(s)	360, 500, 501, 594, 707, 841, 955, 1321
BssHII	GCGCGC	1 cut(s)	200
BssNI	GRCGYC	1 cut(s)	180
BssSI	CACGAG	2 cut(s)	52, 1236
BssT1I	CCWWGG	3 cut(s)	594, 707, 1321
Bst2BI	CACGAG	2 cut(s)	52, 1236
Bst2UI	CCWGG	8 cut(s)	46, 831, 843, 957, 1633, 1961, 2192, 2318
Bst4CI	ACNGT	6 cut(s)	949, 1497, 1577, 1877, 1940, 2147
Bst6I	CTCTTC	3 cut(s)	314, 1239, 1344
BstACI	GRCGYC	1 cut(s)	180
BstAFI	CTTAAG	1 cut(s)	1142
BstC8I	GCNNGC	6 cut(s)	113, 202, 513, 1129, 1638, 2288
BstDEI	CTNAG	5 cut(s)	98, 771, 1371, 1653, 1812
BstDSI	CCRYGG	1 cut(s)	594
BstEII	GGTNACC	2 cut(s)	1571, 2227
BstF5I	GGATG	7 cut(s)	90, 511, 899, 1696, 1763, 1969, 2257
BstFNI	CGCG	5 cut(s)	200, 202, 204, 454, 470
BstH2I	RGCGCY	2 cut(s)	183, 327
BstHHI	GCGC	4 cut(s)	182, 202, 204, 326
BstMAI	GTCTC	6 cut(s)	554, 648, 1341, 1719, 1815, 1841
BstNI	CCWGG	8 cut(s)	46, 831, 843, 957, 1633, 1961, 2192, 2318
BstNSI	RCATGY	1 cut(s)	1431
BstPAI	GACNNNNGTC	1 cut(s)	1063
BstPI	GGTNACC	2 cut(s)	1571, 2227
BstSFI	CTRYAG	3 cut(s)	948, 1033, 1054
BstSLI	GKGCMC	1 cut(s)	113
BstUI	CGCG	5 cut(s)	200, 202, 204, 454, 470
BstV2I	GAAGAC	3 cut(s)	54, 807, 919
BstX2I	RGATCY	6 cut(s)	459, 1006, 1040, 1147, 1741, 2074
BstXI	CCANNNNNNTGG	1 cut(s)	922
BstYI	RGATCY	6 cut(s)	459, 1006, 1040, 1147, 1741, 2074
BsuRI	GGCC	9 cut(s)	33, 58, 365, 376, 387, 494, 706, 954, 2316
BtgI	CCRYGG	1 cut(s)	594
BtgZI	GCGATG	3 cut(s)	654, 821, 1417
BtrI	CACGTC	1 cut(s)	490
BtsCI	GGATG	7 cut(s)	90, 511, 899, 1696, 1763, 1969, 2257
BtsI	GCAGTG	1 cut(s)	2328
BtsIMutI	CAGTG	3 cut(s)	906, 1368, 2328
BtuMI	TCGCGA	1 cut(s)	454
BveI	ACCTGC	2 cut(s)	173, 949
Cac8I	GCNNGC	6 cut(s)	113, 202, 513, 1129, 1638, 2288
CaiI	CAGNNNCTG	2 cut(s)	770, 2317
CciI	TCATGA	4 cut(s)	607, 1173, 1902, 2083
CfoI	GCGC	4 cut(s)	182, 202, 204, 326
Cfr10I	RCCGGY	1 cut(s)	657
Cfr9I	CCCGGG	1 cut(s)	501
CseI	GACGC	2 cut(s)	91, 193
Csp6I	GTAC	3 cut(s)	334, 1389, 1410
CviQI	GTAC	3 cut(s)	334, 1389, 1410
DdeI	CTNAG	5 cut(s)	98, 771, 1371, 1653, 1812
DinI	GGCGCC	1 cut(s)	181
EaeI	YGGCCR	2 cut(s)	56, 492
Eam1104I	CTCTTC	3 cut(s)	314, 1239, 1344
EarI	CTCTTC	3 cut(s)	314, 1239, 1344
Ecl136II	GAGCTC	2 cut(s)	136, 307
Eco130I	CCWWGG	3 cut(s)	594, 707, 1321
Eco147I	AGGCCT	2 cut(s)	33, 706
Eco24I	GRGCYC	3 cut(s)	138, 309, 1321
Eco31I	GGTCTC	2 cut(s)	648, 1815
Eco47I	GGWCC	6 cut(s)	42, 441, 938, 1325, 1940, 2034
Eco53kI	GAGCTC	2 cut(s)	136, 307
Eco57I	CTGAAG	2 cut(s)	542, 659
Eco88I	CYCGRG	3 cut(s)	501, 614, 1668
Eco91I	GGTNACC	2 cut(s)	1571, 2227
EcoICRI	GAGCTC	2 cut(s)	136, 307
EcoO109I	RGGNCCY	2 cut(s)	1325, 2314
EcoO65I	GGTNACC	2 cut(s)	1571, 2227
EcoRI	GAATTC	1 cut(s)	2236
EcoRII	CCWGG	8 cut(s)	44, 829, 841, 955, 1631, 1959, 2190, 2316
EcoT14I	CCWWGG	3 cut(s)	594, 707, 1321
EcoT22I	ATGCAT	2 cut(s)	1087, 1433
EcoT38I	GRGCYC	3 cut(s)	138, 309, 1321
EgeI	GGCGCC	1 cut(s)	181
EheI	GGCGCC	1 cut(s)	181
ErhI	CCWWGG	3 cut(s)	594, 707, 1321
FalI	AAGNNNNNCTT	4 cut(s)	242, 274, 1458, 1490
FauI	CCCGC	4 cut(s)	227, 307, 364, 475
FbaI	TGATCA	1 cut(s)	1120
FblI	GTMKAC	2 cut(s)	207, 486
FokI	GGATG	7 cut(s)	77, 518, 906, 1703, 1750, 1976, 2264
FriOI	GRGCYC	3 cut(s)	138, 309, 1321
FspBI	CTAG	3 cut(s)	708, 1181, 1322
GlaI	GCGC	4 cut(s)	181, 201, 203, 325
GsaI	CCCAGC	1 cut(s)	1599
GsuI	CTGGAG	4 cut(s)	566, 616, 898, 1965
HaeII	RGCGCY	2 cut(s)	183, 327
HaeIII	GGCC	9 cut(s)	33, 58, 365, 376, 387, 494, 706, 954, 2316
HapII	CCGG	6 cut(s)	362, 438, 502, 557, 658, 1534
HgaI	GACGC	2 cut(s)	91, 193
HhaI	GCGC	4 cut(s)	182, 202, 204, 326
Hin1I	GRCGYC	1 cut(s)	180
Hin6I	GCGC	4 cut(s)	180, 200, 202, 324
HinP1I	GCGC	4 cut(s)	180, 200, 202, 324
HincII	GTYRAC	5 cut(s)	208, 487, 852, 1077, 1351
HindII	GTYRAC	5 cut(s)	208, 487, 852, 1077, 1351
HindIII	AAGCTT	1 cut(s)	2057
HpaI	GTTAAC	2 cut(s)	1077, 1351
HpaII	CCGG	6 cut(s)	362, 438, 502, 557, 658, 1534
HphI	GGTGA	8 cut(s)	154, 913, 992, 1196, 1391, 1555, 1572, 1637
Hpy166II	GTNNAC	8 cut(s)	111, 208, 487, 852, 1077, 1351, 1580, 1943
Hpy8I	GTNNAC	8 cut(s)	111, 208, 487, 852, 1077, 1351, 1580, 1943
Hpy99I	CGWCG	7 cut(s)	209, 332, 427, 430, 449, 488, 491
HpyAV	CCTTC	4 cut(s)	23, 268, 840, 884
HpyCH4III	ACNGT	6 cut(s)	949, 1497, 1577, 1877, 1940, 2147
HpyCH4IV	ACGT	3 cut(s)	489, 584, 1697
HpyF3I	CTNAG	5 cut(s)	98, 771, 1371, 1653, 1812
HpySE526I	ACGT	3 cut(s)	489, 584, 1697
Hsp92I	GRCGYC	1 cut(s)	180
HspAI	GCGC	4 cut(s)	180, 200, 202, 324
KasI	GGCGCC	1 cut(s)	179
Kpn2I	TCCGGA	1 cut(s)	1533
KpnI	GGTACC	1 cut(s)	1392
Ksp22I	TGATCA	1 cut(s)	1120
KspAI	GTTAAC	2 cut(s)	1077, 1351
LguI	GCTCTTC	1 cut(s)	314
LmnI	GCTCC	8 cut(s)	141, 173, 239, 547, 635, 857, 917, 1316
MaeI	CTAG	3 cut(s)	708, 1181, 1322
MaeII	ACGT	3 cut(s)	489, 584, 1697
MaeIII	GTNAC	8 cut(s)	160, 580, 1065, 1288, 1543, 1571, 2174, 2227
MauBI	CGCGCGCG	1 cut(s)	200
MbiI	CCGCTC	1 cut(s)	83
MfeI	CAATTG	2 cut(s)	370, 798
MflI	RGATCY	6 cut(s)	459, 1006, 1040, 1147, 1741, 2074
MhlI	GDGCHC	6 cut(s)	113, 138, 309, 632, 908, 1321
MlsI	TGGCCA	1 cut(s)	494
MluNI	TGGCCA	1 cut(s)	494
Mly113I	GGCGCC	1 cut(s)	180
MlyI	GAGTC	4 cut(s)	346, 645, 1196, 1205
MmeI	TCCRAC	5 cut(s)	300, 468, 487, 1791, 2085
Mox20I	TGGCCA	1 cut(s)	494
Mph1103I	ATGCAT	2 cut(s)	1087, 1433
MroI	TCCGGA	1 cut(s)	1533
MroXI	GAANNNNTTC	2 cut(s)	257, 1205
MscI	TGGCCA	1 cut(s)	494
MseI	TTAA	7 cut(s)	699, 1076, 1143, 1305, 1350, 1830, 2169
Msp20I	TGGCCA	1 cut(s)	494
MspA1I	CMGCKG	2 cut(s)	118, 1616
MspCI	CTTAAG	1 cut(s)	1142
MspI	CCGG	6 cut(s)	362, 438, 502, 557, 658, 1534
MunI	CAATTG	2 cut(s)	370, 798
Mva1269I	GAATGC	1 cut(s)	1484
MvaI	CCWGG	8 cut(s)	46, 831, 843, 957, 1633, 1961, 2192, 2318
MvnI	CGCG	5 cut(s)	200, 202, 204, 454, 470
NarI	GGCGCC	1 cut(s)	180
NciI	CCSGG	4 cut(s)	362, 439, 502, 503
NcoI	CCATGG	1 cut(s)	594
NlaIV	GGNNCC	9 cut(s)	181, 442, 461, 913, 1149, 1318, 1390, 2035, 2076
NmeAIII	GCCGAG	3 cut(s)	157, 328, 1460
NmuCI	GTSAC	6 cut(s)	160, 580, 1065, 1288, 1543, 2174
NruI	TCGCGA	1 cut(s)	454
NsiI	ATGCAT	2 cut(s)	1087, 1433
NspI	RCATGY	1 cut(s)	1431
PaeR7I	CTCGAG	2 cut(s)	614, 1668
PagI	TCATGA	4 cut(s)	607, 1173, 1902, 2083
PaqCI	CACCTGC	1 cut(s)	173
PauI	GCGCGC	1 cut(s)	200
PceI	AGGCCT	2 cut(s)	33, 706
PciSI	GCTCTTC	1 cut(s)	314
PcsI	WCGNNNNNNNCGW	4 cut(s)	204, 410, 419, 621
PctI	GAATGC	1 cut(s)	1484
PdmI	GAANNNNTTC	2 cut(s)	257, 1205
PfeI	GAWTC	9 cut(s)	449, 590, 679, 918, 971, 1201, 1530, 2003, 2218
PflFI	GACNNNGTC	1 cut(s)	158
PfoI	TCCNGGA	2 cut(s)	44, 1959
PleI	GAGTC	4 cut(s)	346, 644, 1195, 1204
PluTI	GGCGCC	1 cut(s)	183
PpsI	GAGTC	4 cut(s)	346, 644, 1195, 1204
PpuMI	RGGWCCY	1 cut(s)	1325
PshAI	GACNNNNGTC	1 cut(s)	1063
PshBI	ATTAAT	1 cut(s)	699
Psp124BI	GAGCTC	2 cut(s)	138, 309
Psp5II	RGGWCCY	1 cut(s)	1325
Psp6I	CCWGG	8 cut(s)	44, 829, 841, 955, 1631, 1959, 2190, 2316
PspEI	GGTNACC	2 cut(s)	1571, 2227
PspFI	CCCAGC	1 cut(s)	1595
PspGI	CCWGG	8 cut(s)	44, 829, 841, 955, 1631, 1959, 2190, 2316
PspN4I	GGNNCC	9 cut(s)	181, 442, 461, 913, 1149, 1318, 1390, 2035, 2076
PspPPI	RGGWCCY	1 cut(s)	1325
PstNI	CAGNNNCTG	2 cut(s)	770, 2317
PsuI	RGATCY	6 cut(s)	459, 1006, 1040, 1147, 1741, 2074
PsyI	GACNNNGTC	1 cut(s)	158
PteI	GCGCGC	1 cut(s)	200
PvuII	CAGCTG	1 cut(s)	1616
RruI	TCGCGA	1 cut(s)	454
RsaI	GTAC	3 cut(s)	335, 1390, 1411
RsaNI	GTAC	3 cut(s)	334, 1389, 1410
SacI	GAGCTC	2 cut(s)	138, 309
SalI	GTCGAC	2 cut(s)	206, 485
SapI	GCTCTTC	1 cut(s)	314
SaqAI	TTAA	7 cut(s)	699, 1076, 1143, 1305, 1350, 1830, 2169
SchI	GAGTC	4 cut(s)	346, 645, 1196, 1205
SduI	GDGCHC	6 cut(s)	113, 138, 309, 632, 908, 1321
SfcI	CTRYAG	3 cut(s)	948, 1033, 1054
SfoI	GGCGCC	1 cut(s)	181
Sfr274I	CTCGAG	2 cut(s)	614, 1668
SgrDI	CGTCGACG	1 cut(s)	485
SinI	GGWCC	6 cut(s)	42, 441, 938, 1325, 1940, 2034
SlaI	CTCGAG	2 cut(s)	614, 1668
SmaI	CCCGGG	1 cut(s)	503
SmlI	CTYRAG	4 cut(s)	614, 1142, 1668, 1801
SmoI	CTYRAG	4 cut(s)	614, 1142, 1668, 1801
SseBI	AGGCCT	2 cut(s)	33, 706
SspDI	GGCGCC	1 cut(s)	179
SspI	AATATT	1 cut(s)	1928
SspMI	CTAG	3 cut(s)	708, 1181, 1322
SstI	GAGCTC	2 cut(s)	138, 309
StuI	AGGCCT	2 cut(s)	33, 706
StyI	CCWWGG	3 cut(s)	594, 707, 1321
TaaI	ACNGT	6 cut(s)	949, 1497, 1577, 1877, 1940, 2147
TaiI	ACGT	3 cut(s)	492, 587, 1700
TaqI	TCGA	8 cut(s)	207, 330, 413, 486, 615, 1199, 1602, 1669
TauI	GCSGC	7 cut(s)	83, 118, 180, 387, 518, 875, 1225
TfiI	GAWTC	9 cut(s)	449, 590, 679, 918, 971, 1201, 1530, 2003, 2218
Tru1I	TTAA	7 cut(s)	699, 1076, 1143, 1305, 1350, 1830, 2169
Tru9I	TTAA	7 cut(s)	699, 1076, 1143, 1305, 1350, 1830, 2169
TscAI	CASTG	3 cut(s)	913, 1375, 2328
TseFI	GTSAC	6 cut(s)	160, 580, 1065, 1288, 1543, 2174
Tsp45I	GTSAC	6 cut(s)	160, 580, 1065, 1288, 1543, 2174
TspDTI	ATGAA	6 cut(s)	596, 1162, 1257, 1489, 1780, 2003
TspGWI	ACGGA	2 cut(s)	666, 2264
TspMI	CCCGGG	1 cut(s)	501
TspRI	CASTG	3 cut(s)	913, 1375, 2328
Tth111I	GACNNNGTC	1 cut(s)	158
Vha464I	CTTAAG	1 cut(s)	1142
VneI	GTGCAC	1 cut(s)	109
VpaK11BI	GGWCC	6 cut(s)	42, 441, 938, 1325, 1940, 2034
VspI	ATTAAT	1 cut(s)	699
XapI	RAATTY	3 cut(s)	1718, 2089, 2236
XceI	RCATGY	1 cut(s)	1431
XhoI	CTCGAG	2 cut(s)	614, 1668
XmaI	CCCGGG	1 cut(s)	501
XmaJI	CCTAGG	2 cut(s)	707, 1321
XmiI	GTMKAC	2 cut(s)	207, 486
XmnI	GAANNNNTTC	2 cut(s)	257, 1205
XspI	CTAG	3 cut(s)	708, 1181, 1322
Zsp2I	ATGCAT	2 cut(s)	1087, 1433

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.