Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0008336

Physical Location & Seq

Genomic Coordinates Forward (+)

34720522 .. 34724318

Gene Structure 3797 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00027500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2265 bp

ATGGATTTCGTGGAACTGGAAGCCATAGAAGGCCTCCGATGGTCCTGGAACTCCTGGCCGTCTTCTAAAGCGGACCAAGCCGCTCTCGTCATCCCCCTCAGCGTCATGTGCACGCCGCTGATGCAACAAGCCGAGCTTCCACTCCTCCCCTACGACCCCGTCACATGCTCCCAATGCGGCGCCGTTTTGAACCCCTACGCGCGCGTCGACTACACCTCCCGCATCTGGTTCTGCTCCTTGTGCCTGCACAAGAACCCCTTCCCTCACTCCTACTCCAACATCGGCGACACCAATCTCCCCGCCGAGCTCTTCCCCACTTACAGCGCCGTCGAGTACGCCCGAACCGCCCCGAGTCCCGCCCCGGCCCACAATTGGCCCAACGGCGGCCTCTCGTCCTCGTCCTCGCTGTCCTCGATGGTGTCGTCGTCGCCAAACTCCGGGTCGGACGATTCGCGAGGGATCCGACCCACGTTTGTGTTGGTGGTCGACGTGGCCACGCCCCGGGATGAGCTGGCGGCTCTGAAGAACGAGCTGCTGCTTGCGCTCGAGCAACTGCCGGAGACTGCTCTGGTGGGTTTAGTCACGTTTGATTCCATGGTGCGTGTTCATGATCTCGAGTTTTCCGAGTGCTCCAGAGTCGTTGTGCTTCACGGAGATCGGCAGCTCTCATCGCTTCAGATTCAACAATTTCTGGGCATTAATAGGCCCAGGCAGCAGCAACTTGGAAAAATGCCTTTTTCCCAAAAGCAAGGGTTCTTGCTACCAGTATCTGAGTGTGAGTTCAACATTTCAACTGCAATTGAAGAAATCCAGTCTTCGGCAGTCAAGCCTGGTCATCGCCCAGGAAGGTCAACAGGAGCAGCAATTTCAGCCGCACTTGGACTTTTGGAAGGATGCTTGACTCTGATCTTGGTTATTCCATCAGAACTCACCGAGATCTCATCAATGGTCATGCCCCCTTACTATAGTAGATCTTGCAGCTTCTACAGACATGTGTCACAGAGGTTAACTGATGCATCTATTGTCCTTGATTTGTTTGCTTGTTCTCTTGACCAAGCTGGCTCTGCAGAGCTTAAGGATCCTGTTGAGAGATCGGGTGGTTTCATGATGCTAGGCGAGTCCTTTGAGTCGAATCAGTTCAGAAAATGTTTGCGGCACATTTTTACTCGTGATGAAGAGGGGTATTTGAAGATGTATTTTGATGCAACTATTGAGATAGTGACCACTAAAGATGTTAAAATCTGTGGAGCCCTAGGTCCTTGTGTATCTCTTCGGAAAGTTAACAATTTAGTGAGTAGCACTGAGATTGGTGAGGGTGGTACCTATATATGGAAGTTAGGTACTATTACCAATAAAACATGCATCGCCTTTTTCTTTGAAGTTAGTGATGAGCAGAAAGTTCAACTTGGCTCAGCATTCTTTATACAGTTCATAACACGATACCGACATGGGAACATGGGAATCCGGAAACGGGTGACAACTGCTGCAAGAAGATGGGTTGGTAACCGTTCACCAGAAATTGCTGCTGGGTTCGATCAAGAAACAGCTGCTTCTGTGATAGCCAGGCTTGCTATTCACCGAGCTGAGAATTGTTTTGCTCGAGATGTTATTAGATGGCTGGATGACACGTTGATCCATTTTGCTTCAAAATTTGGAGACTATGTGGAGGAAGATCCATCTTCTTTCCGCTTGGCATCCAACTTCTCTCTTTTTCCCCAATTCATGTATTACTTGAGGAGGTCTCAGTTTATTGATGTCTTTAATAGTAGTCCTGATGAGACAGCGTTCTTTCGGCTGATGCTGAACCGTGAGGGCGTGGTGGGTTCTCTTATCATGATCCAGCCTACACTTCTCCAATATTCCTTTGATGGTCCACCCATTCCAGTCCTCCTGGATGTTCGCTCTATCTCTCCAGATGTTATCTTGCTCTTTGATTCTTACTTTCATGTGGTTATTCACTATGGGTCCAAGATTGCTCAATGGAGGAAGCTTGGTTATGATAAGGATCCAAATCATGAGAATTTGAGGAAGCTGTTGGAAGCCCCAGAAATTGATGCACAGCAGTTGGTGGCTGACCGTGTTCCTGCACCGAAGCTCATTAAATGTGACCAGCATGGTAGCCAGGCAAGGTTTCTTCTTGCAAAGTTGAATCCATCGGTTACCCAGAATTCAACATACACGGATGGCTCGGATATTATCTTGACTGATGATTTGAGCTTGCAAGTATTTTTAGATCACTTGCAGGGCCTGGCAGTGCAAGGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001501 GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005798 GO:0005829 GO:0006810 GO:0006888 GO:0006900 GO:0006996 GO:0007275 GO:0008150 GO:0008270 GO:0009653 GO:0009790 GO:0009792 GO:0009887 GO:0009888 GO:0009987 GO:0012505 GO:0012506 GO:0012507 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0019898 GO:0022607 GO:0030117 GO:0030120 GO:0030127 GO:0030133 GO:0030134 GO:0030135 GO:0030658 GO:0030659 GO:0030660 GO:0030662 GO:0031090 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032991 GO:0033333 GO:0033334 GO:0033339 GO:0034622 GO:0035107 GO:0035138 GO:0035459 GO:0043009 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0046907 GO:0046914 GO:0048193 GO:0048194 GO:0048475 GO:0048513 GO:0048562 GO:0048568 GO:0048598 GO:0048701 GO:0048702 GO:0048704 GO:0048705 GO:0048706 GO:0048731 GO:0048736 GO:0048856 GO:0051179 GO:0051216 GO:0051234 GO:0051641 GO:0051649 GO:0051668 GO:0061024 GO:0061448 GO:0065003 GO:0070971 GO:0071840 GO:0090110 GO:0090114 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:1904888

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

Gelsolin Sec23_BS Sec23_helical Sec23_trunk zf-Sec23_Sec24

Protein Analysis

754

Amino Acids

83.96

Weight (kDa)

5.6

Isoelectric Point (pI)

54.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-Sec23_Sec24	PF04810	53 - 91	1.3e-13	Sec23/Sec24 zinc finger
Sec23_trunk	PF04811	156 - 388	4.6e-35	Sec23/Sec24 trunk domain
Sec23_BS	PF08033	400 - 500	5.6e-19	Sec23/Sec24 beta-sandwich domain
Sec23_helical	PF04815	512 - 609	1.7e-24	Sec23/Sec24 helical domain
Gelsolin	PF00626	626 - 712	3.7e-09	Gelsolin repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008336)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G43670
fragaria_vesca	FvH4_3g28910 FvH4_3g28910 FvH4_3g28910
malus_domestica	MD04G1211400.v1.1 MD12G1226200.v1.1
prunus_persica	Prupe.1G027900_v2.0.a1
pyrus_communis	pycom04g01860 pycom04g18730 pycom04g18740 pycom12g21060 pycom12g21090
rosa_chinensis	RchiOBHm_Chr5g0051941
rosa_laevigata	RLG00000034852
rosa_multiflora	Rmu_co8514153.1_g000001
rosa_roxburghii	Rroxscaffold_1G00027500
rosa_rugosa	Rorug05G0276200 Rorug05G0276300
rosa_samantha	Rh5AG348200 Rh5BG358800 Rh5CG384400 Rh5DG374200
rosa_wichuraiana	Rw5G032860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1319
AccB1I	GGYRCC	2 cut(s)	179, 1319
AccBSI	CCGCTC	1 cut(s)	83
AccI	GTMKAC	2 cut(s)	207, 486
AccII	CGCG	4 cut(s)	200, 202, 204, 454
AccIII	TCCGGA	1 cut(s)	1464
AclWI	GGATC	9 cut(s)	454, 467, 1073, 1086, 1627, 1667, 1831, 2000, 2013
AcoI	YGGCCR	2 cut(s)	56, 492
AcsI	RAATTY	3 cut(s)	1649, 2020, 2167
AcuI	CTGAAG	2 cut(s)	542, 659
AcyI	GRCGYC	1 cut(s)	180
AfaI	GTAC	3 cut(s)	335, 1321, 1342
AfiI	CCNNNNNNNGG	8 cut(s)	225, 372, 383, 437, 501, 817, 1329, 1471
AflII	CTTAAG	1 cut(s)	1073
AflIII	ACRYGT	2 cut(s)	991, 1626
AjiI	CACGTC	1 cut(s)	490
AjnI	CCWGG	9 cut(s)	44, 53, 707, 829, 841, 1562, 1890, 2121, 2247
AleI	CACNNNNGTG	1 cut(s)	473
Alw21I	GWGCWC	3 cut(s)	113, 309, 632
Alw26I	GTCTC	4 cut(s)	554, 1650, 1746, 1772
Alw44I	GTGCAC	1 cut(s)	109
AlwI	GGATC	9 cut(s)	454, 467, 1073, 1086, 1627, 1667, 1831, 2000, 2013
AlwNI	CAGNNNCTG	2 cut(s)	770, 2248
Ama87I	CYCGRG	5 cut(s)	349, 501, 545, 614, 1599
Aor13HI	TCCGGA	1 cut(s)	1464
AoxI	GGCC	8 cut(s)	31, 56, 363, 374, 385, 492, 704, 2245
ApaLI	GTGCAC	1 cut(s)	109
ApoI	RAATTY	3 cut(s)	1649, 2020, 2167
ArsI	GACNNNNNNTTYG	4 cut(s)	425, 457, 2194, 2226
AseI	ATTAAT	1 cut(s)	699
Asp700I	GAANNNNTTC	2 cut(s)	257, 1136
Asp718I	GGTACC	1 cut(s)	1319
AspA2I	CCTAGG	1 cut(s)	1252
AspLEI	GCGC	5 cut(s)	182, 202, 204, 326, 544
AspS9I	GGNCC	9 cut(s)	42, 73, 364, 375, 705, 1256, 1871, 1965, 2245
AsuC2I	CCSGG	4 cut(s)	362, 439, 502, 503
AsuHPI	GGTGA	5 cut(s)	922, 1322, 1486, 1503, 1568
AvaI	CYCGRG	5 cut(s)	349, 501, 545, 614, 1599
AvaII	GGWCC	5 cut(s)	42, 73, 1256, 1871, 1965
AvrII	CCTAGG	1 cut(s)	1252
BaeGI	GKGCMC	1 cut(s)	113
BalI	TGGCCA	1 cut(s)	494
BamHI	GGATCC	3 cut(s)	459, 1078, 2005
BanI	GGYRCC	2 cut(s)	179, 1319
BanII	GRGCYC	2 cut(s)	309, 1252
BauI	CACGAG	1 cut(s)	1167
BbsI	GAAGAC	2 cut(s)	54, 807
Bbv12I	GWGCWC	3 cut(s)	113, 309, 632
BbvCI	CCTCAGC	1 cut(s)	98
BccI	CCATC	9 cut(s)	33, 409, 928, 1488, 1608, 1684, 1862, 2161, 2177
BceAI	ACGGC	4 cut(s)	43, 167, 311, 397
BciT130I	CCWGG	9 cut(s)	46, 55, 709, 831, 843, 1564, 1892, 2123, 2249
BcnI	CCSGG	4 cut(s)	362, 439, 502, 503
BcoDI	GTCTC	4 cut(s)	554, 1650, 1746, 1772
BfaI	CTAG	2 cut(s)	1112, 1253
BfmI	CTRYAG	3 cut(s)	964, 985, 1065
BfoI	RGCGCY	2 cut(s)	183, 327
BfrI	CTTAAG	1 cut(s)	1073
BglII	AGATCT	2 cut(s)	936, 971
BlnI	CCTAGG	1 cut(s)	1252
BlpI	GCTNAGC	1 cut(s)	1411
Bme18I	GGWCC	5 cut(s)	42, 73, 1256, 1871, 1965
BmeT110I	CYCGRG	5 cut(s)	349, 501, 545, 614, 1599
BmgBI	CACGTC	1 cut(s)	490
BmgT120I	GGNCC	9 cut(s)	42, 73, 364, 375, 705, 1256, 1871, 1965, 2245
BmiI	GGNNCC	7 cut(s)	181, 461, 1080, 1249, 1321, 1966, 2007
BoxI	GACNNNNGTC	1 cut(s)	994
BpiI	GAAGAC	2 cut(s)	54, 807
BplI	GAGNNNNNCTC	2 cut(s)	1726, 1758
BpmI	CTGGAG	2 cut(s)	616, 1896
Bpu10I	CCTNAGC	1 cut(s)	98
Bpu1102I	GCTNAGC	1 cut(s)	1411
BpuEI	CTTGAG	1 cut(s)	1753
BpuMI	CCSGG	4 cut(s)	362, 439, 502, 503
BsaBI	GATNNNNATC	2 cut(s)	2004, 2010
BsaHI	GRCGYC	1 cut(s)	180
BsaI	GGTCTC	1 cut(s)	1746
BsaJI	CCNNGG	7 cut(s)	360, 500, 501, 594, 707, 841, 1252
BsaWI	WCCGGW	1 cut(s)	1464
BsaXI	ACNNNNNCTCC	6 cut(s)	200, 230, 257, 287, 1647, 1677
Bsc4I	CCNNNNNNNGG	8 cut(s)	225, 372, 383, 437, 501, 817, 1329, 1471
Bse1I	ACTGG	4 cut(s)	21, 764, 811, 1883
Bse8I	GATNNNNATC	2 cut(s)	2004, 2010
BseAI	TCCGGA	1 cut(s)	1464
BseBI	CCWGG	9 cut(s)	46, 55, 709, 831, 843, 1564, 1892, 2123, 2249
BseDI	CCNNGG	7 cut(s)	360, 500, 501, 594, 707, 841, 1252
BseGI	GGATG	7 cut(s)	90, 511, 899, 1627, 1694, 1900, 2188
BseJI	GATNNNNATC	2 cut(s)	2004, 2010
BseLI	CCNNNNNNNGG	8 cut(s)	225, 372, 383, 437, 501, 817, 1329, 1471
BseMII	CTCAG	6 cut(s)	112, 762, 1293, 1425, 1575, 1757
BseNI	ACTGG	4 cut(s)	21, 764, 811, 1883
BsePI	GCGCGC	1 cut(s)	200
BseRI	GAGGAG	2 cut(s)	134, 1750
BseSI	GKGCMC	1 cut(s)	113
BseYI	CCCAGC	1 cut(s)	1526
BsgI	GTGCAG	2 cut(s)	230, 2070
Bsh1236I	CGCG	4 cut(s)	200, 202, 204, 454
BshFI	GGCC	8 cut(s)	33, 58, 365, 376, 387, 494, 706, 2247
BshNI	GGYRCC	2 cut(s)	179, 1319
BsiHKAI	GWGCWC	3 cut(s)	113, 309, 632
BsiHKCI	CYCGRG	5 cut(s)	349, 501, 545, 614, 1599
BsiSI	CCGG	5 cut(s)	362, 438, 502, 557, 1465
BslFI	GGGAC	1 cut(s)	339
BslI	CCNNNNNNNGG	8 cut(s)	225, 372, 383, 437, 501, 817, 1329, 1471
BsmAI	GTCTC	4 cut(s)	554, 1650, 1746, 1772
BsmFI	GGGAC	1 cut(s)	339
BsmI	GAATGC	1 cut(s)	1415
BsnI	GGCC	8 cut(s)	33, 58, 365, 376, 387, 494, 706, 2247
Bso31I	GGTCTC	1 cut(s)	1746
BsoBI	CYCGRG	5 cut(s)	349, 501, 545, 614, 1599
Bsp1286I	GDGCHC	4 cut(s)	113, 309, 632, 1252
Bsp13I	TCCGGA	1 cut(s)	1464
Bsp1720I	GCTNAGC	1 cut(s)	1411
Bsp19I	CCATGG	1 cut(s)	594
Bsp68I	TCGCGA	1 cut(s)	454
BspANI	GGCC	8 cut(s)	33, 58, 365, 376, 387, 494, 706, 2247
BspCNI	CTCAG	6 cut(s)	111, 763, 1294, 1424, 1576, 1756
BspEI	TCCGGA	1 cut(s)	1464
BspFNI	CGCG	4 cut(s)	200, 202, 204, 454
BspHI	TCATGA	4 cut(s)	607, 1104, 1833, 2014
BspLI	GGNNCC	7 cut(s)	181, 461, 1080, 1249, 1321, 1966, 2007
BspMAI	CTGCAG	1 cut(s)	1069
BspPI	GGATC	9 cut(s)	454, 467, 1073, 1086, 1627, 1667, 1831, 2000, 2013
BspQI	GCTCTTC	1 cut(s)	314
BspT107I	GGYRCC	2 cut(s)	179, 1319
BspTI	CTTAAG	1 cut(s)	1073
BspTNI	GGTCTC	1 cut(s)	1746
BsrBI	CCGCTC	1 cut(s)	83
BsrI	ACTGG	4 cut(s)	21, 764, 811, 1883
BssECI	CCNNGG	7 cut(s)	360, 500, 501, 594, 707, 841, 1252
BssHII	GCGCGC	1 cut(s)	200
BssNI	GRCGYC	1 cut(s)	180
BssSI	CACGAG	1 cut(s)	1167
BssT1I	CCWWGG	2 cut(s)	594, 1252
Bst2BI	CACGAG	1 cut(s)	1167
Bst2UI	CCWGG	9 cut(s)	46, 55, 709, 831, 843, 1564, 1892, 2123, 2249
Bst4CI	ACNGT	4 cut(s)	1428, 1508, 1808, 2078
Bst6I	CTCTTC	3 cut(s)	314, 1170, 1275
BstACI	GRCGYC	1 cut(s)	180
BstAFI	CTTAAG	1 cut(s)	1073
BstC8I	GCNNGC	8 cut(s)	113, 202, 245, 513, 540, 1060, 1569, 2219
BstDEI	CTNAG	6 cut(s)	98, 771, 1302, 1411, 1584, 1743
BstDSI	CCRYGG	1 cut(s)	594
BstEII	GGTNACC	2 cut(s)	1502, 2158
BstF5I	GGATG	7 cut(s)	90, 511, 899, 1627, 1694, 1900, 2188
BstFNI	CGCG	4 cut(s)	200, 202, 204, 454
BstH2I	RGCGCY	2 cut(s)	183, 327
BstHHI	GCGC	5 cut(s)	182, 202, 204, 326, 544
BstMAI	GTCTC	4 cut(s)	554, 1650, 1746, 1772
BstNI	CCWGG	9 cut(s)	46, 55, 709, 831, 843, 1564, 1892, 2123, 2249
BstNSI	RCATGY	3 cut(s)	168, 995, 1362
BstPAI	GACNNNNGTC	1 cut(s)	994
BstPI	GGTNACC	2 cut(s)	1502, 2158
BstSFI	CTRYAG	3 cut(s)	964, 985, 1065
BstSLI	GKGCMC	1 cut(s)	113
BstUI	CGCG	4 cut(s)	200, 202, 204, 454
BstV2I	GAAGAC	2 cut(s)	54, 807
BstX2I	RGATCY	6 cut(s)	459, 936, 971, 1078, 1672, 2005
BstYI	RGATCY	6 cut(s)	459, 936, 971, 1078, 1672, 2005
BsuRI	GGCC	8 cut(s)	33, 58, 365, 376, 387, 494, 706, 2247
BtgI	CCRYGG	1 cut(s)	594
BtgZI	GCGATG	3 cut(s)	654, 821, 1348
BtrI	CACGTC	1 cut(s)	490
BtsCI	GGATG	7 cut(s)	90, 511, 899, 1627, 1694, 1900, 2188
BtsI	GCAGTG	1 cut(s)	2259
BtsIMutI	CAGTG	2 cut(s)	1299, 2259
BtuMI	TCGCGA	1 cut(s)	454
Cac8I	GCNNGC	8 cut(s)	113, 202, 245, 513, 540, 1060, 1569, 2219
CaiI	CAGNNNCTG	2 cut(s)	770, 2248
CciI	TCATGA	4 cut(s)	607, 1104, 1833, 2014
CfoI	GCGC	5 cut(s)	182, 202, 204, 326, 544
Cfr13I	GGNCC	9 cut(s)	42, 73, 364, 375, 705, 1256, 1871, 1965, 2245
Cfr9I	CCCGGG	1 cut(s)	501
CseI	GACGC	2 cut(s)	91, 193
Csp6I	GTAC	3 cut(s)	334, 1320, 1341
CviQI	GTAC	3 cut(s)	334, 1320, 1341
DdeI	CTNAG	6 cut(s)	98, 771, 1302, 1411, 1584, 1743
DinI	GGCGCC	1 cut(s)	181
EaeI	YGGCCR	2 cut(s)	56, 492
Eam1104I	CTCTTC	3 cut(s)	314, 1170, 1275
EarI	CTCTTC	3 cut(s)	314, 1170, 1275
Ecl136II	GAGCTC	1 cut(s)	307
Eco130I	CCWWGG	2 cut(s)	594, 1252
Eco147I	AGGCCT	1 cut(s)	33
Eco24I	GRGCYC	2 cut(s)	309, 1252
Eco31I	GGTCTC	1 cut(s)	1746
Eco47I	GGWCC	5 cut(s)	42, 73, 1256, 1871, 1965
Eco53kI	GAGCTC	1 cut(s)	307
Eco57I	CTGAAG	2 cut(s)	542, 659
Eco88I	CYCGRG	5 cut(s)	349, 501, 545, 614, 1599
Eco91I	GGTNACC	2 cut(s)	1502, 2158
EcoICRI	GAGCTC	1 cut(s)	307
EcoO109I	RGGNCCY	2 cut(s)	1256, 2245
EcoO65I	GGTNACC	2 cut(s)	1502, 2158
EcoRI	GAATTC	1 cut(s)	2167
EcoRII	CCWGG	9 cut(s)	44, 53, 707, 829, 841, 1562, 1890, 2121, 2247
EcoT14I	CCWWGG	2 cut(s)	594, 1252
EcoT22I	ATGCAT	2 cut(s)	1018, 1364
EcoT38I	GRGCYC	2 cut(s)	309, 1252
EgeI	GGCGCC	1 cut(s)	181
EheI	GGCGCC	1 cut(s)	181
ErhI	CCWWGG	2 cut(s)	594, 1252
FalI	AAGNNNNNCTT	6 cut(s)	120, 152, 242, 274, 1389, 1421
FaqI	GGGAC	1 cut(s)	339
FauI	CCCGC	3 cut(s)	227, 307, 364
FblI	GTMKAC	2 cut(s)	207, 486
FokI	GGATG	7 cut(s)	77, 518, 906, 1634, 1681, 1907, 2195
FriOI	GRGCYC	2 cut(s)	309, 1252
FspBI	CTAG	2 cut(s)	1112, 1253
GlaI	GCGC	5 cut(s)	181, 201, 203, 325, 543
GsaI	CCCAGC	1 cut(s)	1530
GsuI	CTGGAG	2 cut(s)	616, 1896
HaeII	RGCGCY	2 cut(s)	183, 327
HaeIII	GGCC	8 cut(s)	33, 58, 365, 376, 387, 494, 706, 2247
HapII	CCGG	5 cut(s)	362, 438, 502, 557, 1465
HgaI	GACGC	2 cut(s)	91, 193
HhaI	GCGC	5 cut(s)	182, 202, 204, 326, 544
Hin1I	GRCGYC	1 cut(s)	180
Hin6I	GCGC	5 cut(s)	180, 200, 202, 324, 542
HinP1I	GCGC	5 cut(s)	180, 200, 202, 324, 542
HincII	GTYRAC	5 cut(s)	208, 487, 852, 1008, 1282
HindII	GTYRAC	5 cut(s)	208, 487, 852, 1008, 1282
HindIII	AAGCTT	1 cut(s)	1988
HpaI	GTTAAC	2 cut(s)	1008, 1282
HpaII	CCGG	5 cut(s)	362, 438, 502, 557, 1465
HphI	GGTGA	5 cut(s)	922, 1322, 1486, 1503, 1568
Hpy166II	GTNNAC	8 cut(s)	111, 208, 487, 852, 1008, 1282, 1511, 1874
Hpy8I	GTNNAC	8 cut(s)	111, 208, 487, 852, 1008, 1282, 1511, 1874
Hpy99I	CGWCG	5 cut(s)	209, 332, 427, 430, 491
HpyAV	CCTTC	4 cut(s)	23, 268, 840, 884
HpyCH4III	ACNGT	4 cut(s)	1428, 1508, 1808, 2078
HpyCH4IV	ACGT	4 cut(s)	470, 489, 584, 1628
HpyF3I	CTNAG	6 cut(s)	98, 771, 1302, 1411, 1584, 1743
HpySE526I	ACGT	4 cut(s)	470, 489, 584, 1628
Hsp92I	GRCGYC	1 cut(s)	180
HspAI	GCGC	5 cut(s)	180, 200, 202, 324, 542
KasI	GGCGCC	1 cut(s)	179
Kpn2I	TCCGGA	1 cut(s)	1464
KpnI	GGTACC	1 cut(s)	1323
KspAI	GTTAAC	2 cut(s)	1008, 1282
LguI	GCTCTTC	1 cut(s)	314
LmnI	GCTCC	5 cut(s)	173, 239, 635, 857, 1247
MaeI	CTAG	2 cut(s)	1112, 1253
MaeII	ACGT	4 cut(s)	470, 489, 584, 1628
MaeIII	GTNAC	8 cut(s)	160, 580, 996, 1219, 1474, 1502, 2105, 2158
MauBI	CGCGCGCG	1 cut(s)	200
MbiI	CCGCTC	1 cut(s)	83
MfeI	CAATTG	2 cut(s)	370, 798
MflI	RGATCY	6 cut(s)	459, 936, 971, 1078, 1672, 2005
MhlI	GDGCHC	4 cut(s)	113, 309, 632, 1252
MlsI	TGGCCA	1 cut(s)	494
MluNI	TGGCCA	1 cut(s)	494
Mly113I	GGCGCC	1 cut(s)	180
MlyI	GAGTC	5 cut(s)	361, 645, 895, 1127, 1136
MmeI	TCCRAC	5 cut(s)	300, 423, 487, 1722, 2016
Mox20I	TGGCCA	1 cut(s)	494
Mph1103I	ATGCAT	2 cut(s)	1018, 1364
MroI	TCCGGA	1 cut(s)	1464
MroXI	GAANNNNTTC	2 cut(s)	257, 1136
MscI	TGGCCA	1 cut(s)	494
MseI	TTAA	7 cut(s)	699, 1007, 1074, 1236, 1281, 1761, 2100
MslI	CAYNNNNRTG	1 cut(s)	473
Msp20I	TGGCCA	1 cut(s)	494
MspA1I	CMGCKG	2 cut(s)	118, 1547
MspCI	CTTAAG	1 cut(s)	1073
MspI	CCGG	5 cut(s)	362, 438, 502, 557, 1465
MunI	CAATTG	2 cut(s)	370, 798
Mva1269I	GAATGC	1 cut(s)	1415
MvaI	CCWGG	9 cut(s)	46, 55, 709, 831, 843, 1564, 1892, 2123, 2249
MvnI	CGCG	4 cut(s)	200, 202, 204, 454
NarI	GGCGCC	1 cut(s)	180
NciI	CCSGG	4 cut(s)	362, 439, 502, 503
NcoI	CCATGG	1 cut(s)	594
NlaIV	GGNNCC	7 cut(s)	181, 461, 1080, 1249, 1321, 1966, 2007
NmeAIII	GCCGAG	2 cut(s)	157, 328
NmuCI	GTSAC	6 cut(s)	160, 580, 996, 1219, 1474, 2105
NruI	TCGCGA	1 cut(s)	454
NsiI	ATGCAT	2 cut(s)	1018, 1364
NspI	RCATGY	3 cut(s)	168, 995, 1362
OliI	CACNNNNGTG	1 cut(s)	473
PaeR7I	CTCGAG	3 cut(s)	545, 614, 1599
PagI	TCATGA	4 cut(s)	607, 1104, 1833, 2014
PauI	GCGCGC	1 cut(s)	200
PceI	AGGCCT	1 cut(s)	33
PciI	ACATGT	1 cut(s)	991
PciSI	GCTCTTC	1 cut(s)	314
PcsI	WCGNNNNNNNCGW	4 cut(s)	204, 410, 419, 621
PctI	GAATGC	1 cut(s)	1415
PdmI	GAANNNNTTC	2 cut(s)	257, 1136
PfeI	GAWTC	7 cut(s)	449, 590, 679, 1132, 1461, 1934, 2149
PflFI	GACNNNGTC	1 cut(s)	158
PfoI	TCCNGGA	2 cut(s)	44, 1890
PleI	GAGTC	5 cut(s)	360, 644, 895, 1126, 1135
PluTI	GGCGCC	1 cut(s)	183
PpsI	GAGTC	5 cut(s)	360, 644, 895, 1126, 1135
PpuMI	RGGWCCY	1 cut(s)	1256
PscI	ACATGT	1 cut(s)	991
PshAI	GACNNNNGTC	1 cut(s)	994
PshBI	ATTAAT	1 cut(s)	699
Psp124BI	GAGCTC	1 cut(s)	309
Psp5II	RGGWCCY	1 cut(s)	1256
Psp6I	CCWGG	9 cut(s)	44, 53, 707, 829, 841, 1562, 1890, 2121, 2247
PspEI	GGTNACC	2 cut(s)	1502, 2158
PspFI	CCCAGC	1 cut(s)	1526
PspGI	CCWGG	9 cut(s)	44, 53, 707, 829, 841, 1562, 1890, 2121, 2247
PspN4I	GGNNCC	7 cut(s)	181, 461, 1080, 1249, 1321, 1966, 2007
PspPI	GGNCC	9 cut(s)	42, 73, 364, 375, 705, 1256, 1871, 1965, 2245
PspPPI	RGGWCCY	1 cut(s)	1256
PspXI	VCTCGAGB	1 cut(s)	545
PstI	CTGCAG	1 cut(s)	1069
PstNI	CAGNNNCTG	2 cut(s)	770, 2248
PsuI	RGATCY	6 cut(s)	459, 936, 971, 1078, 1672, 2005
PsyI	GACNNNGTC	1 cut(s)	158
PteI	GCGCGC	1 cut(s)	200
PvuII	CAGCTG	1 cut(s)	1547
RruI	TCGCGA	1 cut(s)	454
RsaI	GTAC	3 cut(s)	335, 1321, 1342
RsaNI	GTAC	3 cut(s)	334, 1320, 1341
RseI	CAYNNNNRTG	1 cut(s)	473
SacI	GAGCTC	1 cut(s)	309
SalI	GTCGAC	2 cut(s)	206, 485
SapI	GCTCTTC	1 cut(s)	314
SaqAI	TTAA	7 cut(s)	699, 1007, 1074, 1236, 1281, 1761, 2100
Sau96I	GGNCC	9 cut(s)	42, 73, 364, 375, 705, 1256, 1871, 1965, 2245
SchI	GAGTC	5 cut(s)	361, 645, 895, 1127, 1136
SduI	GDGCHC	4 cut(s)	113, 309, 632, 1252
SfcI	CTRYAG	3 cut(s)	964, 985, 1065
SfoI	GGCGCC	1 cut(s)	181
Sfr274I	CTCGAG	3 cut(s)	545, 614, 1599
SinI	GGWCC	5 cut(s)	42, 73, 1256, 1871, 1965
SlaI	CTCGAG	3 cut(s)	545, 614, 1599
SmaI	CCCGGG	1 cut(s)	503
SmiMI	CAYNNNNRTG	1 cut(s)	473
SmlI	CTYRAG	5 cut(s)	545, 614, 1073, 1599, 1732
SmoI	CTYRAG	5 cut(s)	545, 614, 1073, 1599, 1732
SseBI	AGGCCT	1 cut(s)	33
SspDI	GGCGCC	1 cut(s)	179
SspI	AATATT	1 cut(s)	1859
SspMI	CTAG	2 cut(s)	1112, 1253
SstI	GAGCTC	1 cut(s)	309
StuI	AGGCCT	1 cut(s)	33
StyI	CCWWGG	2 cut(s)	594, 1252
TaaI	ACNGT	4 cut(s)	1428, 1508, 1808, 2078
TaiI	ACGT	4 cut(s)	473, 492, 587, 1631
TaqI	TCGA	9 cut(s)	207, 330, 413, 486, 546, 615, 1130, 1533, 1600
TauI	GCSGC	7 cut(s)	83, 118, 180, 387, 518, 875, 1156
TfiI	GAWTC	7 cut(s)	449, 590, 679, 1132, 1461, 1934, 2149
Tru1I	TTAA	7 cut(s)	699, 1007, 1074, 1236, 1281, 1761, 2100
Tru9I	TTAA	7 cut(s)	699, 1007, 1074, 1236, 1281, 1761, 2100
TscAI	CASTG	2 cut(s)	1306, 2259
TseFI	GTSAC	6 cut(s)	160, 580, 996, 1219, 1474, 2105
Tsp45I	GTSAC	6 cut(s)	160, 580, 996, 1219, 1474, 2105
TspDTI	ATGAA	6 cut(s)	596, 1093, 1188, 1420, 1711, 1934
TspGWI	ACGGA	2 cut(s)	666, 2195
TspMI	CCCGGG	1 cut(s)	501
TspRI	CASTG	2 cut(s)	1306, 2259
Tth111I	GACNNNGTC	1 cut(s)	158
Vha464I	CTTAAG	1 cut(s)	1073
VneI	GTGCAC	1 cut(s)	109
VpaK11BI	GGWCC	5 cut(s)	42, 73, 1256, 1871, 1965
VspI	ATTAAT	1 cut(s)	699
XapI	RAATTY	3 cut(s)	1649, 2020, 2167
XceI	RCATGY	3 cut(s)	168, 995, 1362
XcmI	CCANNNNNNNNNTGG	1 cut(s)	475
XhoI	CTCGAG	3 cut(s)	545, 614, 1599
XmaI	CCCGGG	1 cut(s)	501
XmaJI	CCTAGG	1 cut(s)	1252
XmiI	GTMKAC	2 cut(s)	207, 486
XmnI	GAANNNNTTC	2 cut(s)	257, 1136
XspI	CTAG	2 cut(s)	1112, 1253
Zsp2I	ATGCAT	2 cut(s)	1018, 1364

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.