Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0008336

Physical Location & Seq

Genomic Coordinates Reverse (-)

55686758 .. 55692096

Gene Structure 5339 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G032860.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2157 bp

ATGCGGCGCCGTTTTGAACCCCTACGCGCGCGTCGACTACACCTCCCGCATCTGGTTCTGCTCCTTGTGCCTCCACAAGAACCCCTTCCCTCACTCCTACTCCAACATCGGCGACACCAATCTCCCCGCCGAGCTCTTCCCCACTTACAGCGCCGTCGAGTACGCCCGAACCGCCCCGACTCCGCCCCGGCCCACAATTGGCCCAACGGCGGCCTGTCCTCGTCCTCGCTGTCCTCGATGGTGTCGTCGTCGCCAAACTCCGGGTCCGACGATTCGCGAGGGATCCGACCCGCGTTTGTGTTGGTCGTCGACGTGGCCACGCCCCGGGATGAGCTGGCGGCTCTGAAGAACGAGCTGCTGCTTGTGCTGGAGCAACTGCCGGAGACTGCTCTGGTGGGTTTAGTCACGTTTGATTCCATGGTGCGTGTTCATGATCTCGAGTTTTCCGAGTGCTCCAGAGTCGTTGTGCTTCACGGAGACCGGCAGCTCTCATCGCTTCAGATTCAACAATTTCTGGGCATTAATAGGCCTAGGCAGCAGCAACTCGGAAAAATGCCTTTTTCCCAAAAGCAAGGGTTCTTGCTACCAGTATCTGAGTGTGAGTTCAACATTTCAACTGCAATTGAAGAAATCCAGTCTTCGGCAGTCAAGCCTGGTCATCGCCCAGGAAGGTCAACAGGAGCAGCAATTTCAGCCGCACTTGGACTTTTGGAAGGATGCTTGGTGAGCACTGGCTCCAGAATCATGGTCTTCACATCTGGACCTGCAACTGTAGGCCCAGGAATTATTGTAGATTCTGATCTTGGTTATTCCATCAGAACTCACCGAGATCTCATCAATGGTCATGCCCCTTACTATAGTAGATCTTGCAGCTTCTACAGACAAGTGTCACAGAGGTTAACTGATGCATCTATTGTCCTTGATTTGTTTGCTTGTTCTCTTGATCAAGCTGGCTCTGCGGAACTTAAGGATCCTGTTGAGAGATCGGGTGGTTTCATGATGCTAGGTGAGTCCTTTGAGTCGAATCAGTTCAGAAAATGTTTGCGGCACATTTTTACTCGTGATGAAGAGGGGTATTTGAAGATGTATTTTGATGCAACTATTGAGATAGTGACCACTAAAGATGTTAAAATCTGTGGAGCCCTAGGTCCTTGTGTATCTCTTCGGAAAGTTAACAATTTAGTGAGTAGCACTGAGATTGGTGAGGGTGGTACCTATATATGGAAGTTAGGTACTATTACCAATAAAACATGCATCGCCTTTTTCTTTGAAGTTAGTGCTGAGCAGAAAGTTCAACTTGGCTCGGCATTCTTTATACAGTTCATAACACGATACCGACATGGGAACATGGGAATCCGGAAACGGGTGACAACTGCTGCAAGAAGATGGGTTGGTAACCGTTCACCAGAAATTGCTGCTGGGTTCGATCAAGAAACAGCTGCTTCTGTGATAGCCAGGCTTGCTATTCACCGAGCTGAGAATTGTTTTGCTCGAGATGTTATTAGATGGTTGGATGACACGTTGATCCATTTTGCTTCAAAATTTGGAGACTATGTGGAGGAAGATCCATCTTCTTTCCGCTTGGCATCCAACTTCTCTCTTTTTCCCCAATTCATGTATTACTTGAGGAGGTCTCAGTTTATTGATGTCTTTAATAGTAGCCCTGATGAGACAGCTTTCTTTCGGCTGATGCTGAACCGTGAGGGCGTGGTGGGTTCTCTTATCATGATCCAGCCTACACTTCTCCAATATTCCTTTGACGGTCCACCCATTCCAGTCCTCCTGGATGTTCGCTCTATCTCTCCAGATGTTATCTTGCTCTTTGATTCTTACTTTCATGTGGTTATTCACTATGGGTCCAAGATTGCTCAATGGAGGAAGCTTGGTTATGATAAGGATCCAAATCATGAGAATTTGAGGAAGCTGTTGGAAGCCCCAGAAATTGATGCACAGCAATTGGTGGCTGACCGTGTTCCTGCACCGAAGCTCATTAAATGTGACCAGCATGGTAGCCAGGCAAGGTTTCTTCTTGCAAAGTTGAATCCATCGGTTACCCAGAATTCAACATACACGGATGGCTCGGATATTATCTTGACTGATGATTTGAGCTTGCAAGTATTTTTAGATCACTTGCAGGGCCTGGCAGTGCAAGGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001501 GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005798 GO:0005829 GO:0006810 GO:0006888 GO:0006900 GO:0006996 GO:0007275 GO:0008150 GO:0008270 GO:0009653 GO:0009790 GO:0009792 GO:0009887 GO:0009888 GO:0009987 GO:0012505 GO:0012506 GO:0012507 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0019898 GO:0022607 GO:0030117 GO:0030120 GO:0030127 GO:0030133 GO:0030134 GO:0030135 GO:0030658 GO:0030659 GO:0030660 GO:0030662 GO:0031090 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032991 GO:0033333 GO:0033334 GO:0033339 GO:0034622 GO:0035107 GO:0035138 GO:0035459 GO:0043009 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0046907 GO:0046914 GO:0048193 GO:0048194 GO:0048475 GO:0048513 GO:0048562 GO:0048568 GO:0048598 GO:0048701 GO:0048702 GO:0048704 GO:0048705 GO:0048706 GO:0048731 GO:0048736 GO:0048856 GO:0051179 GO:0051216 GO:0051234 GO:0051641 GO:0051649 GO:0051668 GO:0061024 GO:0061448 GO:0065003 GO:0070971 GO:0071840 GO:0090110 GO:0090114 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:1904888

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

Gelsolin Sec23_BS Sec23_helical Sec23_trunk zf-Sec23_Sec24

Protein Analysis

718

Amino Acids

80.27

Weight (kDa)

8.54

Isoelectric Point (pI)

58.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Sec23_trunk	PF04811	97 - 352	2.1e-50	Sec23/Sec24 trunk domain
Sec23_BS	PF08033	364 - 464	6e-19	Sec23/Sec24 beta-sandwich domain
Sec23_helical	PF04815	476 - 573	1.6e-24	Sec23/Sec24 helical domain
Gelsolin	PF00626	590 - 676	3.4e-09	Gelsolin repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0008336)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G43670
fragaria_vesca	FvH4_3g28910 FvH4_3g28910 FvH4_3g28910
malus_domestica	MD04G1211400.v1.1 MD12G1226200.v1.1
prunus_persica	Prupe.1G027900_v2.0.a1
pyrus_communis	pycom04g01860 pycom04g18730 pycom04g18740 pycom12g21060 pycom12g21090
rosa_chinensis	RchiOBHm_Chr5g0051941
rosa_laevigata	RLG00000034852
rosa_multiflora	Rmu_co8514153.1_g000001
rosa_roxburghii	Rroxscaffold_1G00027500
rosa_rugosa	Rorug05G0276200 Rorug05G0276300
rosa_samantha	Rh5AG348200 Rh5BG358800 Rh5CG384400 Rh5DG374200
rosa_wichuraiana	Rw5G032860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	772
Acc65I	GGTACC	1 cut(s)	1211
AccB1I	GGYRCC	2 cut(s)	6, 1211
AccI	GTMKAC	2 cut(s)	34, 309
AccII	CGCG	5 cut(s)	27, 29, 31, 277, 293
AccIII	TCCGGA	1 cut(s)	1356
AclWI	GGATC	9 cut(s)	277, 290, 965, 978, 1519, 1559, 1723, 1892, 1905
AcoI	YGGCCR	1 cut(s)	315
AcsI	RAATTY	3 cut(s)	1541, 1912, 2059
AcuI	CTGAAG	2 cut(s)	365, 482
AcyI	GRCGYC	1 cut(s)	7
AfaI	GTAC	3 cut(s)	162, 1213, 1234
AfiI	CCNNNNNNNGG	8 cut(s)	52, 198, 209, 260, 324, 640, 1221, 1363
AflII	CTTAAG	1 cut(s)	965
AflIII	ACRYGT	1 cut(s)	1518
AjiI	CACGTC	1 cut(s)	313
AjnI	CCWGG	7 cut(s)	652, 664, 778, 1454, 1782, 2013, 2139
Alw21I	GWGCWC	3 cut(s)	136, 455, 731
Alw26I	GTCTC	5 cut(s)	377, 471, 1542, 1638, 1664
AlwI	GGATC	9 cut(s)	277, 290, 965, 978, 1519, 1559, 1723, 1892, 1905
AlwNI	CAGNNNCTG	2 cut(s)	593, 2140
Ama87I	CYCGRG	3 cut(s)	324, 437, 1491
Aor13HI	TCCGGA	1 cut(s)	1356
AoxI	GGCC	7 cut(s)	189, 200, 211, 315, 527, 775, 2137
ApoI	RAATTY	3 cut(s)	1541, 1912, 2059
ArsI	GACNNNNNNTTYG	4 cut(s)	248, 280, 2086, 2118
AseI	ATTAAT	1 cut(s)	522
Asp700I	GAANNNNTTC	2 cut(s)	84, 1028
Asp718I	GGTACC	1 cut(s)	1211
AspA2I	CCTAGG	2 cut(s)	530, 1144
AspLEI	GCGC	4 cut(s)	9, 29, 31, 153
AspS9I	GGNCC	9 cut(s)	190, 201, 264, 761, 776, 1148, 1763, 1857, 2137
AsuC2I	CCSGG	4 cut(s)	188, 262, 325, 326
AsuHPI	GGTGA	7 cut(s)	736, 815, 1019, 1214, 1378, 1395, 1460
AvaI	CYCGRG	3 cut(s)	324, 437, 1491
AvaII	GGWCC	5 cut(s)	264, 761, 1148, 1763, 1857
AvrII	CCTAGG	2 cut(s)	530, 1144
BalI	TGGCCA	1 cut(s)	317
BamHI	GGATCC	3 cut(s)	282, 970, 1897
BanI	GGYRCC	2 cut(s)	6, 1211
BanII	GRGCYC	2 cut(s)	136, 1144
BauI	CACGAG	1 cut(s)	1059
BbsI	GAAGAC	2 cut(s)	630, 742
Bbv12I	GWGCWC	3 cut(s)	136, 455, 731
BccI	CCATC	7 cut(s)	232, 821, 1380, 1500, 1576, 2053, 2069
BceAI	ACGGC	2 cut(s)	138, 223
BciT130I	CCWGG	7 cut(s)	654, 666, 780, 1456, 1784, 2015, 2141
BclI	TGATCA	1 cut(s)	943
BcnI	CCSGG	4 cut(s)	188, 262, 325, 326
BcoDI	GTCTC	5 cut(s)	377, 471, 1542, 1638, 1664
BfaI	CTAG	3 cut(s)	531, 1004, 1145
BfmI	CTRYAG	3 cut(s)	771, 856, 877
BfoI	RGCGCY	2 cut(s)	10, 154
BfrI	CTTAAG	1 cut(s)	965
BfuAI	ACCTGC	1 cut(s)	772
BglII	AGATCT	2 cut(s)	829, 863
BlnI	CCTAGG	2 cut(s)	530, 1144
BlpI	GCTNAGC	1 cut(s)	1281
Bme18I	GGWCC	5 cut(s)	264, 761, 1148, 1763, 1857
BmeT110I	CYCGRG	3 cut(s)	324, 437, 1491
BmgBI	CACGTC	1 cut(s)	313
BmgT120I	GGNCC	9 cut(s)	190, 201, 264, 761, 776, 1148, 1763, 1857, 2137
BmiI	GGNNCC	9 cut(s)	8, 265, 284, 736, 972, 1141, 1213, 1858, 1899
BoxI	GACNNNNGTC	1 cut(s)	886
BpiI	GAAGAC	2 cut(s)	630, 742
BplI	GAGNNNNNCTC	2 cut(s)	1618, 1650
BpmI	CTGGAG	4 cut(s)	389, 439, 721, 1788
Bpu1102I	GCTNAGC	1 cut(s)	1281
BpuEI	CTTGAG	1 cut(s)	1645
BpuMI	CCSGG	4 cut(s)	188, 262, 325, 326
BsaBI	GATNNNNATC	3 cut(s)	798, 1896, 1902
BsaHI	GRCGYC	1 cut(s)	7
BsaI	GGTCTC	2 cut(s)	471, 1638
BsaJI	CCNNGG	8 cut(s)	186, 323, 324, 417, 530, 664, 778, 1144
BsaWI	WCCGGW	1 cut(s)	1356
BsaXI	ACNNNNNCTCC	6 cut(s)	27, 57, 84, 114, 1539, 1569
Bsc4I	CCNNNNNNNGG	8 cut(s)	52, 198, 209, 260, 324, 640, 1221, 1363
Bse118I	RCCGGY	1 cut(s)	480
Bse1I	ACTGG	4 cut(s)	587, 634, 736, 1775
Bse8I	GATNNNNATC	3 cut(s)	798, 1896, 1902
BseAI	TCCGGA	1 cut(s)	1356
BseBI	CCWGG	7 cut(s)	654, 666, 780, 1456, 1784, 2015, 2141
BseDI	CCNNGG	8 cut(s)	186, 323, 324, 417, 530, 664, 778, 1144
BseGI	GGATG	6 cut(s)	334, 722, 1519, 1586, 1792, 2080
BseJI	GATNNNNATC	3 cut(s)	798, 1896, 1902
BseLI	CCNNNNNNNGG	8 cut(s)	52, 198, 209, 260, 324, 640, 1221, 1363
BseMII	CTCAG	5 cut(s)	585, 1185, 1272, 1467, 1649
BseNI	ACTGG	4 cut(s)	587, 634, 736, 1775
BsePI	GCGCGC	1 cut(s)	27
BseRI	GAGGAG	1 cut(s)	1642
BseYI	CCCAGC	1 cut(s)	1418
BsgI	GTGCAG	1 cut(s)	1962
Bsh1236I	CGCG	5 cut(s)	27, 29, 31, 277, 293
BshFI	GGCC	7 cut(s)	191, 202, 213, 317, 529, 777, 2139
BshNI	GGYRCC	2 cut(s)	6, 1211
BsiHKAI	GWGCWC	3 cut(s)	136, 455, 731
BsiHKCI	CYCGRG	3 cut(s)	324, 437, 1491
BsiSI	CCGG	6 cut(s)	188, 261, 325, 380, 481, 1357
BslI	CCNNNNNNNGG	8 cut(s)	52, 198, 209, 260, 324, 640, 1221, 1363
BsmAI	GTCTC	5 cut(s)	377, 471, 1542, 1638, 1664
BsmI	GAATGC	1 cut(s)	1307
BsnI	GGCC	7 cut(s)	191, 202, 213, 317, 529, 777, 2139
Bso31I	GGTCTC	2 cut(s)	471, 1638
BsoBI	CYCGRG	3 cut(s)	324, 437, 1491
Bsp1286I	GDGCHC	4 cut(s)	136, 455, 731, 1144
Bsp13I	TCCGGA	1 cut(s)	1356
Bsp1720I	GCTNAGC	1 cut(s)	1281
Bsp19I	CCATGG	1 cut(s)	417
Bsp68I	TCGCGA	1 cut(s)	277
BspANI	GGCC	7 cut(s)	191, 202, 213, 317, 529, 777, 2139
BspCNI	CTCAG	5 cut(s)	586, 1186, 1273, 1468, 1648
BspEI	TCCGGA	1 cut(s)	1356
BspFNI	CGCG	5 cut(s)	27, 29, 31, 277, 293
BspHI	TCATGA	4 cut(s)	430, 996, 1725, 1906
BspLI	GGNNCC	9 cut(s)	8, 265, 284, 736, 972, 1141, 1213, 1858, 1899
BspMI	ACCTGC	1 cut(s)	772
BspPI	GGATC	9 cut(s)	277, 290, 965, 978, 1519, 1559, 1723, 1892, 1905
BspQI	GCTCTTC	1 cut(s)	141
BspT107I	GGYRCC	2 cut(s)	6, 1211
BspTI	CTTAAG	1 cut(s)	965
BspTNI	GGTCTC	2 cut(s)	471, 1638
BsrFI	RCCGGY	1 cut(s)	480
BsrI	ACTGG	4 cut(s)	587, 634, 736, 1775
BssAI	RCCGGY	1 cut(s)	480
BssECI	CCNNGG	8 cut(s)	186, 323, 324, 417, 530, 664, 778, 1144
BssHII	GCGCGC	1 cut(s)	27
BssNI	GRCGYC	1 cut(s)	7
BssSI	CACGAG	1 cut(s)	1059
BssT1I	CCWWGG	3 cut(s)	417, 530, 1144
Bst2BI	CACGAG	1 cut(s)	1059
Bst2UI	CCWGG	7 cut(s)	654, 666, 780, 1456, 1784, 2015, 2141
Bst4CI	ACNGT	6 cut(s)	772, 1320, 1400, 1700, 1763, 1970
Bst6I	CTCTTC	3 cut(s)	141, 1062, 1167
BstACI	GRCGYC	1 cut(s)	7
BstAFI	CTTAAG	1 cut(s)	965
BstC8I	GCNNGC	5 cut(s)	29, 336, 952, 1461, 2111
BstDEI	CTNAG	5 cut(s)	594, 1194, 1281, 1476, 1635
BstDSI	CCRYGG	1 cut(s)	417
BstEII	GGTNACC	2 cut(s)	1394, 2050
BstF5I	GGATG	6 cut(s)	334, 722, 1519, 1586, 1792, 2080
BstFNI	CGCG	5 cut(s)	27, 29, 31, 277, 293
BstH2I	RGCGCY	2 cut(s)	10, 154
BstHHI	GCGC	4 cut(s)	9, 29, 31, 153
BstMAI	GTCTC	5 cut(s)	377, 471, 1542, 1638, 1664
BstMWI	GCNNNNNNNGC	9 cut(s)	67, 171, 364, 493, 535, 692, 726, 956, 1460
BstNI	CCWGG	7 cut(s)	654, 666, 780, 1456, 1784, 2015, 2141
BstNSI	RCATGY	1 cut(s)	1254
BstPAI	GACNNNNGTC	1 cut(s)	886
BstPI	GGTNACC	2 cut(s)	1394, 2050
BstSFI	CTRYAG	3 cut(s)	771, 856, 877
BstUI	CGCG	5 cut(s)	27, 29, 31, 277, 293
BstV2I	GAAGAC	2 cut(s)	630, 742
BstX2I	RGATCY	6 cut(s)	282, 829, 863, 970, 1564, 1897
BstXI	CCANNNNNNTGG	1 cut(s)	745
BstYI	RGATCY	6 cut(s)	282, 829, 863, 970, 1564, 1897
BsuRI	GGCC	7 cut(s)	191, 202, 213, 317, 529, 777, 2139
BtgI	CCRYGG	1 cut(s)	417
BtgZI	GCGATG	3 cut(s)	477, 644, 1240
BtrI	CACGTC	1 cut(s)	313
BtsCI	GGATG	6 cut(s)	334, 722, 1519, 1586, 1792, 2080
BtsI	GCAGTG	1 cut(s)	2151
BtsIMutI	CAGTG	3 cut(s)	729, 1191, 2151
BtuMI	TCGCGA	1 cut(s)	277
BveI	ACCTGC	1 cut(s)	772
Cac8I	GCNNGC	5 cut(s)	29, 336, 952, 1461, 2111
CaiI	CAGNNNCTG	2 cut(s)	593, 2140
CciI	TCATGA	4 cut(s)	430, 996, 1725, 1906
CfoI	GCGC	4 cut(s)	9, 29, 31, 153
Cfr10I	RCCGGY	1 cut(s)	480
Cfr13I	GGNCC	9 cut(s)	190, 201, 264, 761, 776, 1148, 1763, 1857, 2137
Cfr9I	CCCGGG	1 cut(s)	324
CseI	GACGC	1 cut(s)	20
Csp6I	GTAC	3 cut(s)	161, 1212, 1233
CviQI	GTAC	3 cut(s)	161, 1212, 1233
DdeI	CTNAG	5 cut(s)	594, 1194, 1281, 1476, 1635
DinI	GGCGCC	1 cut(s)	8
EaeI	YGGCCR	1 cut(s)	315
Eam1104I	CTCTTC	3 cut(s)	141, 1062, 1167
EarI	CTCTTC	3 cut(s)	141, 1062, 1167
EciI	GGCGGA	1 cut(s)	172
Ecl136II	GAGCTC	1 cut(s)	134
Eco130I	CCWWGG	3 cut(s)	417, 530, 1144
Eco147I	AGGCCT	1 cut(s)	529
Eco24I	GRGCYC	2 cut(s)	136, 1144
Eco31I	GGTCTC	2 cut(s)	471, 1638
Eco47I	GGWCC	5 cut(s)	264, 761, 1148, 1763, 1857
Eco53kI	GAGCTC	1 cut(s)	134
Eco57I	CTGAAG	2 cut(s)	365, 482
Eco88I	CYCGRG	3 cut(s)	324, 437, 1491
Eco91I	GGTNACC	2 cut(s)	1394, 2050
EcoICRI	GAGCTC	1 cut(s)	134
EcoO109I	RGGNCCY	2 cut(s)	1148, 2137
EcoO65I	GGTNACC	2 cut(s)	1394, 2050
EcoRI	GAATTC	1 cut(s)	2059
EcoRII	CCWGG	7 cut(s)	652, 664, 778, 1454, 1782, 2013, 2139
EcoT14I	CCWWGG	3 cut(s)	417, 530, 1144
EcoT22I	ATGCAT	2 cut(s)	910, 1256
EcoT38I	GRGCYC	2 cut(s)	136, 1144
EgeI	GGCGCC	1 cut(s)	8
EheI	GGCGCC	1 cut(s)	8
ErhI	CCWWGG	3 cut(s)	417, 530, 1144
FalI	AAGNNNNNCTT	4 cut(s)	69, 101, 1281, 1313
FauI	CCCGC	3 cut(s)	54, 134, 298
FbaI	TGATCA	1 cut(s)	943
FblI	GTMKAC	2 cut(s)	34, 309
FokI	GGATG	6 cut(s)	341, 729, 1526, 1573, 1799, 2087
FriOI	GRGCYC	2 cut(s)	136, 1144
FspBI	CTAG	3 cut(s)	531, 1004, 1145
GlaI	GCGC	4 cut(s)	8, 28, 30, 152
GsaI	CCCAGC	1 cut(s)	1422
GsuI	CTGGAG	4 cut(s)	389, 439, 721, 1788
HaeII	RGCGCY	2 cut(s)	10, 154
HaeIII	GGCC	7 cut(s)	191, 202, 213, 317, 529, 777, 2139
HapII	CCGG	6 cut(s)	188, 261, 325, 380, 481, 1357
HgaI	GACGC	1 cut(s)	20
HhaI	GCGC	4 cut(s)	9, 29, 31, 153
Hin1I	GRCGYC	1 cut(s)	7
Hin6I	GCGC	4 cut(s)	7, 27, 29, 151
HinP1I	GCGC	4 cut(s)	7, 27, 29, 151
HincII	GTYRAC	5 cut(s)	35, 310, 675, 900, 1174
HindII	GTYRAC	5 cut(s)	35, 310, 675, 900, 1174
HindIII	AAGCTT	1 cut(s)	1880
HpaI	GTTAAC	2 cut(s)	900, 1174
HpaII	CCGG	6 cut(s)	188, 261, 325, 380, 481, 1357
HphI	GGTGA	7 cut(s)	736, 815, 1019, 1214, 1378, 1395, 1460
Hpy166II	GTNNAC	7 cut(s)	35, 310, 675, 900, 1174, 1403, 1766
Hpy8I	GTNNAC	7 cut(s)	35, 310, 675, 900, 1174, 1403, 1766
Hpy99I	CGWCG	7 cut(s)	36, 159, 250, 253, 272, 311, 314
HpyAV	CCTTC	3 cut(s)	95, 663, 707
HpyCH4III	ACNGT	6 cut(s)	772, 1320, 1400, 1700, 1763, 1970
HpyCH4IV	ACGT	3 cut(s)	312, 407, 1520
HpyF10VI	GCNNNNNNNGC	9 cut(s)	67, 171, 364, 493, 535, 692, 726, 956, 1460
HpyF3I	CTNAG	5 cut(s)	594, 1194, 1281, 1476, 1635
HpySE526I	ACGT	3 cut(s)	312, 407, 1520
Hsp92I	GRCGYC	1 cut(s)	7
HspAI	GCGC	4 cut(s)	7, 27, 29, 151
KasI	GGCGCC	1 cut(s)	6
Kpn2I	TCCGGA	1 cut(s)	1356
KpnI	GGTACC	1 cut(s)	1215
Ksp22I	TGATCA	1 cut(s)	943
KspAI	GTTAAC	2 cut(s)	900, 1174
LguI	GCTCTTC	1 cut(s)	141
LmnI	GCTCC	6 cut(s)	66, 370, 458, 680, 740, 1139
MaeI	CTAG	3 cut(s)	531, 1004, 1145
MaeII	ACGT	3 cut(s)	312, 407, 1520
MaeIII	GTNAC	7 cut(s)	403, 888, 1111, 1366, 1394, 1997, 2050
MauBI	CGCGCGCG	1 cut(s)	27
MfeI	CAATTG	3 cut(s)	196, 621, 1955
MflI	RGATCY	6 cut(s)	282, 829, 863, 970, 1564, 1897
MhlI	GDGCHC	4 cut(s)	136, 455, 731, 1144
MlsI	TGGCCA	1 cut(s)	317
MluNI	TGGCCA	1 cut(s)	317
Mly113I	GGCGCC	1 cut(s)	7
MlyI	GAGTC	4 cut(s)	173, 468, 1019, 1028
MmeI	TCCRAC	6 cut(s)	127, 291, 310, 1491, 1614, 1908
Mox20I	TGGCCA	1 cut(s)	317
Mph1103I	ATGCAT	2 cut(s)	910, 1256
MroI	TCCGGA	1 cut(s)	1356
MroXI	GAANNNNTTC	2 cut(s)	84, 1028
MscI	TGGCCA	1 cut(s)	317
MseI	TTAA	7 cut(s)	522, 899, 966, 1128, 1173, 1653, 1992
Msp20I	TGGCCA	1 cut(s)	317
MspA1I	CMGCKG	1 cut(s)	1439
MspCI	CTTAAG	1 cut(s)	965
MspI	CCGG	6 cut(s)	188, 261, 325, 380, 481, 1357
MunI	CAATTG	3 cut(s)	196, 621, 1955
Mva1269I	GAATGC	1 cut(s)	1307
MvaI	CCWGG	7 cut(s)	654, 666, 780, 1456, 1784, 2015, 2141
MvnI	CGCG	5 cut(s)	27, 29, 31, 277, 293
MwoI	GCNNNNNNNGC	9 cut(s)	67, 171, 364, 493, 535, 692, 726, 956, 1460
NarI	GGCGCC	1 cut(s)	7
NciI	CCSGG	4 cut(s)	188, 262, 325, 326
NcoI	CCATGG	1 cut(s)	417
NlaIV	GGNNCC	9 cut(s)	8, 265, 284, 736, 972, 1141, 1213, 1858, 1899
NmeAIII	GCCGAG	2 cut(s)	155, 1283
NmuCI	GTSAC	5 cut(s)	403, 888, 1111, 1366, 1997
NruI	TCGCGA	1 cut(s)	277
NsiI	ATGCAT	2 cut(s)	910, 1256
NspI	RCATGY	1 cut(s)	1254
PaeR7I	CTCGAG	2 cut(s)	437, 1491
PagI	TCATGA	4 cut(s)	430, 996, 1725, 1906
PauI	GCGCGC	1 cut(s)	27
PceI	AGGCCT	1 cut(s)	529
PciSI	GCTCTTC	1 cut(s)	141
PcsI	WCGNNNNNNNCGW	4 cut(s)	31, 233, 242, 444
PctI	GAATGC	1 cut(s)	1307
PdmI	GAANNNNTTC	2 cut(s)	84, 1028
PfeI	GAWTC	9 cut(s)	272, 413, 502, 741, 794, 1024, 1353, 1826, 2041
PfoI	TCCNGGA	1 cut(s)	1782
PleI	GAGTC	4 cut(s)	173, 467, 1018, 1027
PluTI	GGCGCC	1 cut(s)	10
PpsI	GAGTC	4 cut(s)	173, 467, 1018, 1027
PpuMI	RGGWCCY	1 cut(s)	1148
PshAI	GACNNNNGTC	1 cut(s)	886
PshBI	ATTAAT	1 cut(s)	522
Psp124BI	GAGCTC	1 cut(s)	136
Psp5II	RGGWCCY	1 cut(s)	1148
Psp6I	CCWGG	7 cut(s)	652, 664, 778, 1454, 1782, 2013, 2139
PspEI	GGTNACC	2 cut(s)	1394, 2050
PspFI	CCCAGC	1 cut(s)	1418
PspGI	CCWGG	7 cut(s)	652, 664, 778, 1454, 1782, 2013, 2139
PspN4I	GGNNCC	9 cut(s)	8, 265, 284, 736, 972, 1141, 1213, 1858, 1899
PspPI	GGNCC	9 cut(s)	190, 201, 264, 761, 776, 1148, 1763, 1857, 2137
PspPPI	RGGWCCY	1 cut(s)	1148
PstNI	CAGNNNCTG	2 cut(s)	593, 2140
PsuI	RGATCY	6 cut(s)	282, 829, 863, 970, 1564, 1897
PteI	GCGCGC	1 cut(s)	27
PvuII	CAGCTG	1 cut(s)	1439
RruI	TCGCGA	1 cut(s)	277
RsaI	GTAC	3 cut(s)	162, 1213, 1234
RsaNI	GTAC	3 cut(s)	161, 1212, 1233
SacI	GAGCTC	1 cut(s)	136
SalI	GTCGAC	2 cut(s)	33, 308
SapI	GCTCTTC	1 cut(s)	141
SaqAI	TTAA	7 cut(s)	522, 899, 966, 1128, 1173, 1653, 1992
Sau96I	GGNCC	9 cut(s)	190, 201, 264, 761, 776, 1148, 1763, 1857, 2137
SchI	GAGTC	4 cut(s)	173, 468, 1019, 1028
SduI	GDGCHC	4 cut(s)	136, 455, 731, 1144
SfcI	CTRYAG	3 cut(s)	771, 856, 877
SfoI	GGCGCC	1 cut(s)	8
Sfr274I	CTCGAG	2 cut(s)	437, 1491
SgrDI	CGTCGACG	1 cut(s)	308
SinI	GGWCC	5 cut(s)	264, 761, 1148, 1763, 1857
SlaI	CTCGAG	2 cut(s)	437, 1491
SmaI	CCCGGG	1 cut(s)	326
SmlI	CTYRAG	4 cut(s)	437, 965, 1491, 1624
SmoI	CTYRAG	4 cut(s)	437, 965, 1491, 1624
SseBI	AGGCCT	1 cut(s)	529
SspDI	GGCGCC	1 cut(s)	6
SspI	AATATT	1 cut(s)	1751
SspMI	CTAG	3 cut(s)	531, 1004, 1145
SstI	GAGCTC	1 cut(s)	136
StuI	AGGCCT	1 cut(s)	529
StyI	CCWWGG	3 cut(s)	417, 530, 1144
TaaI	ACNGT	6 cut(s)	772, 1320, 1400, 1700, 1763, 1970
TaiI	ACGT	3 cut(s)	315, 410, 1523
TaqI	TCGA	8 cut(s)	34, 157, 236, 309, 438, 1022, 1425, 1492
TauI	GCSGC	5 cut(s)	7, 213, 341, 698, 1048
TfiI	GAWTC	9 cut(s)	272, 413, 502, 741, 794, 1024, 1353, 1826, 2041
Tru1I	TTAA	7 cut(s)	522, 899, 966, 1128, 1173, 1653, 1992
Tru9I	TTAA	7 cut(s)	522, 899, 966, 1128, 1173, 1653, 1992
TscAI	CASTG	3 cut(s)	736, 1198, 2151
TseFI	GTSAC	5 cut(s)	403, 888, 1111, 1366, 1997
Tsp45I	GTSAC	5 cut(s)	403, 888, 1111, 1366, 1997
TspDTI	ATGAA	6 cut(s)	419, 985, 1080, 1312, 1603, 1826
TspGWI	ACGGA	2 cut(s)	489, 2087
TspMI	CCCGGG	1 cut(s)	324
TspRI	CASTG	3 cut(s)	736, 1198, 2151
Vha464I	CTTAAG	1 cut(s)	965
VpaK11BI	GGWCC	5 cut(s)	264, 761, 1148, 1763, 1857
VspI	ATTAAT	1 cut(s)	522
XapI	RAATTY	3 cut(s)	1541, 1912, 2059
XceI	RCATGY	1 cut(s)	1254
XhoI	CTCGAG	2 cut(s)	437, 1491
XmaI	CCCGGG	1 cut(s)	324
XmaJI	CCTAGG	2 cut(s)	530, 1144
XmiI	GTMKAC	2 cut(s)	34, 309
XmnI	GAANNNNTTC	2 cut(s)	84, 1028
XspI	CTAG	3 cut(s)	531, 1004, 1145
Zsp2I	ATGCAT	2 cut(s)	910, 1256

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.