Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0008336

Physical Location & Seq

Genomic Coordinates Forward (+)

22333323 .. 22335915

Gene Structure 2593 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom12g21060.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1842 bp

ATGGATTTCGTGGAACTGGAGGCGATCGAGGGCCTCCGATGGTCCTGGAATTCATGGCCGCCGTCAAAATCCGAGTGCGCCGCCCTTGTCATCCCTCTCAGCGTCATGTGCACACCGTTGATGCAGCAAGCCGAGCTCCCGATACTCCCCTACGACCCCGTCACTTGCCTCCGATGCCGCGCCGTTTTGAACCCCTACGCCCGCGTCGATTACCCTTCTCGCATCTGGTTCTGCTCCTTCTGCCACCACAAGAACTCCTTCCCTCACTCTTACTCCAACATCGGCGAGACCAACCTCCCCGCCGAGCTTTTTCCGACTTACAGCGCCGTAGAGTACGCCAAAACCGCCCATGGAATTCCCAATTCTGGCTCGAATTCCCAGAATTGGGCAAGTGGGTTGTCGTCATCGTCGTCCCTGTCGTCTATGGGGTCGTCGGCGGCGGCGTCGTCGGTGTCGAATGGGGATTCGAGAGGGGTGAGGCCGGCGTTTGTGTTCGTTGTGGATGCTTGCACGCCTGGGGACGAGCTTGGGGCACTTAAAAATGAGCTCTTGCTTGTTGTGGAGAAGCTTCCGGAGACCGCTTTGGTGGGTCTGATCACGTTTGATTCGATGGTTCGCGTTCATGATCTCGGGTTTTCGGAGTGCTCGAGGGCCGTGATGCTTCACGGCGAACGGGAGCTGTCATCGCTTCAGACCCAACAATTTCTAGGCATTAAGCGACCAAAGCATCAGCAACTCGGAAAGATATCAGTTAATCAAAAGCAGGGCTTTTTGCTACCTGTTTCTGAATGTGAGTTCAACATCACAACTGCAATTGAAGAGATTCATTGTTCAGTTCAAGTCAAGCCCGGTCATCGTCCTCAAAGGTGTACCGGAGCAGCAATATCAGCTGCACTTGGACTTCTGGAAGGATGTTCAGGGAGCACGGGTTCCAGGATCATGGTCTTCACATCTGGACCAGCAACTTTAGGCCCAGGAATAATCGTTGATTCCGATCTTAGCAAGTCCATCAGAAATCACCGAGACCTCATCAATGGTCATGCCCCTTACTATAGTAGATCTTGCAGCTTTTATAGGCAAGTGTCGCAGAGGTTAACTGATGCATCTATTGTTCTCGATTTGTTTGCTTGTTCTCTTGATCAAGCTGGAGTTGCAGAGCTGAAGGTCCCTGTTGAGAAGTCAGGTGGTTTCATGATGCTAGGTGAGTCATTTGCGTCAAATCAATTCAGAAAATGTTTAAGCCACATTTTTACTCGTGATAAAGGGGGATATTTGAAGATGTATTTTGATGCAACTATTGAGATAGTAACCACTAGAGATATTAAAATCTGTGGAGCCCTAGGTCCTTGTGTATCTCTTCGAGAAGTCAATGATTTAGTGAGTAGCAGTGAGATTGGGGAGGGTGGTACCTATATGTGGAAGTTGGGTACAATCACCAATAAAACATGCATTGCTTTTTTCTTCCGAGTGAGTGATGAGCAGAAAGTTCAACCTGGGTCGGCATTCCTGATACAGTTCATAATAAGATACCGTCATGGGAACATGGGGATCCGGAAAAGGGTAACAACTGCAGCGAGAAGATGGGTTGGGGGCAGTTCACCAGAAATTGCTGCTGGGTTTGATCAAGAAGCTGCAGCTTCAGTGATAGCCAGGCTTGCTATTCGCCGAGCTGAGACTTGTTTCGCTCGAGATGTTATCAGATGGCTGGATGACACATTGATCCGCTTTGCTTCCAAGTTTGGAGACTATGCGGAGGAACGATCCAACTTCTTTCCGCCTGTCATCCAACTTCTCCCTTTTCCCTCAGTTCATGTATTACTTAAGGAGGTCTCAGTTTATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001501 GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005798 GO:0005829 GO:0006810 GO:0006888 GO:0006900 GO:0006996 GO:0007275 GO:0008150 GO:0008270 GO:0009653 GO:0009790 GO:0009792 GO:0009887 GO:0009888 GO:0009987 GO:0012505 GO:0012506 GO:0012507 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0019898 GO:0022607 GO:0030117 GO:0030120 GO:0030127 GO:0030133 GO:0030134 GO:0030135 GO:0030658 GO:0030659 GO:0030660 GO:0030662 GO:0031090 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032991 GO:0033333 GO:0033334 GO:0033339 GO:0034622 GO:0035107 GO:0035138 GO:0035459 GO:0043009 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0046907 GO:0046914 GO:0048193 GO:0048194 GO:0048475 GO:0048513 GO:0048562 GO:0048568 GO:0048598 GO:0048701 GO:0048702 GO:0048704 GO:0048705 GO:0048706 GO:0048731 GO:0048736 GO:0048856 GO:0051179 GO:0051216 GO:0051234 GO:0051641 GO:0051649 GO:0051668 GO:0061024 GO:0061448 GO:0065003 GO:0070971 GO:0071840 GO:0090110 GO:0090114 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:1904888

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

Gelsolin Sec23_BS Sec23_helical Sec23_trunk zf-Sec23_Sec24

Protein Analysis

614

Amino Acids

67.28

Weight (kDa)

7.8

Isoelectric Point (pI)

49.55

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008336)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G43670
fragaria_vesca	FvH4_3g28910 FvH4_3g28910 FvH4_3g28910
malus_domestica	MD04G1211400.v1.1 MD12G1226200.v1.1
prunus_persica	Prupe.1G027900_v2.0.a1
pyrus_communis	pycom04g01860 pycom04g18730 pycom04g18740 pycom12g21060 pycom12g21090
rosa_chinensis	RchiOBHm_Chr5g0051941
rosa_laevigata	RLG00000034852
rosa_multiflora	Rmu_co8514153.1_g000001
rosa_roxburghii	Rroxscaffold_1G00027500
rosa_rugosa	Rorug05G0276200 Rorug05G0276300
rosa_samantha	Rh5AG348200 Rh5BG358800 Rh5CG384400 Rh5DG374200
rosa_wichuraiana	Rw5G032860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1406
AccB1I	GGYRCC	1 cut(s)	1406
AccII	CGCG	3 cut(s)	180, 204, 618
AccIII	TCCGGA	2 cut(s)	571, 1551
AclWI	GGATC	5 cut(s)	944, 1543, 1556, 1714, 1755
AcoI	YGGCCR	1 cut(s)	56
AcsI	RAATTY	3 cut(s)	49, 354, 373
AcuI	CTGAAG	3 cut(s)	674, 1181, 1623
AcyI	GRCGYC	1 cut(s)	443
AfaI	GTAC	4 cut(s)	335, 871, 1408, 1429
AfiI	CCNNNNNNNGG	5 cut(s)	365, 384, 385, 1416, 1558
AflII	CTTAAG	1 cut(s)	1820
AgsI	TTSAA	6 cut(s)	190, 799, 818, 839, 1276, 1490
AjnI	CCWGG	6 cut(s)	44, 514, 932, 973, 1492, 1649
AloI	GAACNNNNNNTCC	2 cut(s)	913, 945
Alw21I	GWGCWC	5 cut(s)	113, 138, 549, 647, 926
Alw26I	GTCTC	6 cut(s)	281, 569, 1017, 1667, 1737, 1834
Alw44I	GTGCAC	1 cut(s)	109
AlwI	GGATC	5 cut(s)	944, 1543, 1556, 1714, 1755
Ama87I	CYCGRG	3 cut(s)	629, 646, 1686
Aor13HI	TCCGGA	2 cut(s)	571, 1551
AoxI	GGCC	5 cut(s)	31, 56, 479, 651, 970
ApaLI	GTGCAC	1 cut(s)	109
ApeKI	GCWGC	8 cut(s)	124, 878, 890, 1065, 1571, 1610, 1631, 1634
ApoI	RAATTY	3 cut(s)	49, 354, 373
Asp700I	GAANNNNTTC	2 cut(s)	257, 822
Asp718I	GGTACC	1 cut(s)	1406
AspA2I	CCTAGG	1 cut(s)	1339
AspLEI	GCGC	3 cut(s)	80, 182, 326
AspS9I	GGNCC	7 cut(s)	31, 42, 651, 956, 971, 1165, 1343
AsuC2I	CCSGG	1 cut(s)	849
AsuHPI	GGTGA	5 cut(s)	487, 1010, 1214, 1426, 1590
AvaI	CYCGRG	3 cut(s)	629, 646, 1686
AvaII	GGWCC	4 cut(s)	42, 956, 1165, 1343
AvrII	CCTAGG	1 cut(s)	1339
BaeGI	GKGCMC	2 cut(s)	113, 535
BamHI	GGATCC	1 cut(s)	1548
BanI	GGYRCC	1 cut(s)	1406
BanII	GRGCYC	3 cut(s)	138, 549, 1339
BauI	CACGAG	1 cut(s)	1254
BbsI	GAAGAC	1 cut(s)	937
Bbv12I	GWGCWC	5 cut(s)	113, 138, 549, 647, 926
BbvI	GCAGC	8 cut(s)	136, 877, 890, 1077, 1583, 1597, 1618, 1646
BccI	CCATC	5 cut(s)	33, 604, 1016, 1575, 1695
BceAI	ACGGC	5 cut(s)	46, 167, 311, 638, 682
BcgI	CGANNNNNNTGC	2 cut(s)	1106, 1140
BciT130I	CCWGG	6 cut(s)	46, 516, 934, 975, 1494, 1651
BclI	TGATCA	3 cut(s)	594, 1138, 1621
BcnI	CCSGG	1 cut(s)	849
BcoDI	GTCTC	6 cut(s)	281, 569, 1017, 1667, 1737, 1834
BfaI	CTAG	4 cut(s)	707, 1199, 1314, 1340
BfmI	CTRYAG	3 cut(s)	1051, 1569, 1632
BfoI	RGCGCY	1 cut(s)	327
BfrI	CTTAAG	1 cut(s)	1820
BglII	AGATCT	1 cut(s)	1058
BlnI	CCTAGG	1 cut(s)	1339
Bme1390I	CCNGG	7 cut(s)	46, 516, 849, 934, 975, 1494, 1651
Bme18I	GGWCC	4 cut(s)	42, 956, 1165, 1343
BmeT110I	CYCGRG	3 cut(s)	629, 646, 1686
BmgT120I	GGNCC	7 cut(s)	31, 42, 651, 956, 971, 1165, 1343
BmiI	GGNNCC	5 cut(s)	931, 1167, 1336, 1408, 1550
BmrFI	CCNGG	7 cut(s)	46, 516, 849, 934, 975, 1494, 1651
BpiI	GAAGAC	1 cut(s)	937
BpmI	CTGGAG	2 cut(s)	38, 1167
BpuMI	CCSGG	1 cut(s)	849
BsaBI	GATNNNNATC	1 cut(s)	993
BsaHI	GRCGYC	1 cut(s)	443
BsaI	GGTCTC	4 cut(s)	281, 569, 1017, 1834
BsaJI	CCNNGG	5 cut(s)	349, 515, 973, 1339, 1493
BsaWI	WCCGGW	3 cut(s)	571, 872, 1551
BsaXI	ACNNNNNCTCC	6 cut(s)	239, 257, 269, 287, 913, 943
Bsc4I	CCNNNNNNNGG	5 cut(s)	365, 384, 385, 1416, 1558
Bse118I	RCCGGY	1 cut(s)	481
Bse1I	ACTGG	1 cut(s)	21
Bse3DI	GCAATG	1 cut(s)	1449
Bse8I	GATNNNNATC	1 cut(s)	993
BseAI	TCCGGA	2 cut(s)	571, 1551
BseBI	CCWGG	6 cut(s)	46, 516, 934, 975, 1494, 1651
BseDI	CCNNGG	5 cut(s)	349, 515, 973, 1339, 1493
BseGI	GGATG	5 cut(s)	90, 508, 917, 1714, 1782
BseJI	GATNNNNATC	1 cut(s)	993
BseLI	CCNNNNNNNGG	5 cut(s)	365, 384, 385, 1416, 1558
BseMI	GCAATG	1 cut(s)	1449
BseMII	CTCAG	3 cut(s)	112, 1662, 1818
BseNI	ACTGG	1 cut(s)	21
BseSI	GKGCMC	2 cut(s)	113, 535
BseXI	GCAGC	8 cut(s)	136, 877, 890, 1077, 1583, 1597, 1618, 1646
BseYI	CCCAGC	1 cut(s)	1613
BsgI	GTGCAG	1 cut(s)	876
Bsh1236I	CGCG	3 cut(s)	180, 204, 618
Bsh1285I	CGRYCG	1 cut(s)	27
BshFI	GGCC	5 cut(s)	33, 58, 481, 653, 972
BshNI	GGYRCC	1 cut(s)	1406
BsiEI	CGRYCG	1 cut(s)	27
BsiHKAI	GWGCWC	5 cut(s)	113, 138, 549, 647, 926
BsiHKCI	CYCGRG	3 cut(s)	629, 646, 1686
BsiSI	CCGG	5 cut(s)	482, 572, 849, 873, 1552
BslFI	GGGAC	3 cut(s)	397, 533, 1151
BslI	CCNNNNNNNGG	5 cut(s)	365, 384, 385, 1416, 1558
BsmAI	GTCTC	6 cut(s)	281, 569, 1017, 1667, 1737, 1834
BsmFI	GGGAC	3 cut(s)	397, 533, 1151
BsmI	GAATGC	1 cut(s)	1502
BsnI	GGCC	5 cut(s)	33, 58, 481, 653, 972
Bso31I	GGTCTC	4 cut(s)	281, 569, 1017, 1834
BsoBI	CYCGRG	3 cut(s)	629, 646, 1686
Bsp1286I	GDGCHC	7 cut(s)	113, 138, 535, 549, 647, 926, 1339
Bsp13I	TCCGGA	2 cut(s)	571, 1551
Bsp19I	CCATGG	1 cut(s)	349
BspANI	GGCC	5 cut(s)	33, 58, 481, 653, 972
BspCNI	CTCAG	3 cut(s)	111, 1663, 1817
BspEI	TCCGGA	2 cut(s)	571, 1551
BspFNI	CGCG	3 cut(s)	180, 204, 618
BspHI	TCATGA	2 cut(s)	622, 1191
BspLI	GGNNCC	5 cut(s)	931, 1167, 1336, 1408, 1550
BspMAI	CTGCAG	2 cut(s)	1573, 1636
BspPI	GGATC	5 cut(s)	944, 1543, 1556, 1714, 1755
BspT107I	GGYRCC	1 cut(s)	1406
BspTI	CTTAAG	1 cut(s)	1820
BspTNI	GGTCTC	4 cut(s)	281, 569, 1017, 1834
BsrDI	GCAATG	1 cut(s)	1449
BsrFI	RCCGGY	1 cut(s)	481
BsrI	ACTGG	1 cut(s)	21
BssAI	RCCGGY	1 cut(s)	481
BssECI	CCNNGG	5 cut(s)	349, 515, 973, 1339, 1493
BssNI	GRCGYC	1 cut(s)	443
BssSI	CACGAG	1 cut(s)	1254
BssT1I	CCWWGG	2 cut(s)	349, 1339
Bst2BI	CACGAG	1 cut(s)	1254
Bst2UI	CCWGG	6 cut(s)	46, 516, 934, 975, 1494, 1651
Bst4CI	ACNGT	3 cut(s)	117, 1515, 1532
Bst6I	CTCTTC	2 cut(s)	813, 1362
BstACI	GRCGYC	1 cut(s)	443
BstAFI	CTTAAG	1 cut(s)	1820
BstC8I	GCNNGC	6 cut(s)	129, 202, 483, 508, 512, 1656
BstDEI	CTNAG	5 cut(s)	98, 998, 1671, 1804, 1831
BstDSI	CCRYGG	1 cut(s)	349
BstF5I	GGATG	5 cut(s)	90, 508, 917, 1714, 1782
BstFNI	CGCG	3 cut(s)	180, 204, 618
BstH2I	RGCGCY	1 cut(s)	327
BstHHI	GCGC	3 cut(s)	80, 182, 326
BstMAI	GTCTC	6 cut(s)	281, 569, 1017, 1667, 1737, 1834
BstMCI	CGRYCG	1 cut(s)	27
BstNI	CCWGG	6 cut(s)	46, 516, 934, 975, 1494, 1651
BstNSI	RCATGY	1 cut(s)	1449
BstSCI	CCNGG	7 cut(s)	44, 514, 847, 932, 973, 1492, 1649
BstSFI	CTRYAG	3 cut(s)	1051, 1569, 1632
BstSLI	GKGCMC	2 cut(s)	113, 535
BstUI	CGCG	3 cut(s)	180, 204, 618
BstV1I	GCAGC	8 cut(s)	136, 877, 890, 1077, 1583, 1597, 1618, 1646
BstV2I	GAAGAC	1 cut(s)	937
BstX2I	RGATCY	2 cut(s)	1058, 1548
BstXI	CCANNNNNNTGG	1 cut(s)	940
BstYI	RGATCY	2 cut(s)	1058, 1548
BsuRI	GGCC	5 cut(s)	33, 58, 481, 653, 972
BtgI	CCRYGG	1 cut(s)	349
BtgZI	GCGATG	1 cut(s)	669
BtsCI	GGATG	5 cut(s)	90, 508, 917, 1714, 1782
BtsI	GCAGTG	1 cut(s)	1393
BtsIMutI	CAGTG	2 cut(s)	1393, 1647
Cac8I	GCNNGC	6 cut(s)	129, 202, 483, 508, 512, 1656
CciI	TCATGA	2 cut(s)	622, 1191
CfoI	GCGC	3 cut(s)	80, 182, 326
Cfr10I	RCCGGY	1 cut(s)	481
Cfr13I	GGNCC	7 cut(s)	31, 42, 651, 956, 971, 1165, 1343
CseI	GACGC	4 cut(s)	91, 193, 432, 1203
Csp6I	GTAC	4 cut(s)	334, 870, 1407, 1428
CviQI	GTAC	4 cut(s)	334, 870, 1407, 1428
DdeI	CTNAG	5 cut(s)	98, 998, 1671, 1804, 1831
EaeI	YGGCCR	1 cut(s)	56
Eam1104I	CTCTTC	2 cut(s)	813, 1362
EarI	CTCTTC	2 cut(s)	813, 1362
EciI	GGCGGA	1 cut(s)	1764
Ecl136II	GAGCTC	2 cut(s)	136, 547
Eco130I	CCWWGG	2 cut(s)	349, 1339
Eco24I	GRGCYC	3 cut(s)	138, 549, 1339
Eco31I	GGTCTC	4 cut(s)	281, 569, 1017, 1834
Eco32I	GATATC	1 cut(s)	747
Eco47I	GGWCC	4 cut(s)	42, 956, 1165, 1343
Eco53kI	GAGCTC	2 cut(s)	136, 547
Eco57I	CTGAAG	3 cut(s)	674, 1181, 1623
Eco88I	CYCGRG	3 cut(s)	629, 646, 1686
EcoICRI	GAGCTC	2 cut(s)	136, 547
EcoO109I	RGGNCCY	3 cut(s)	31, 1165, 1343
EcoRI	GAATTC	3 cut(s)	49, 354, 373
EcoRII	CCWGG	6 cut(s)	44, 514, 932, 973, 1492, 1649
EcoRV	GATATC	1 cut(s)	747
EcoT14I	CCWWGG	2 cut(s)	349, 1339
EcoT22I	ATGCAT	2 cut(s)	1105, 1451
EcoT38I	GRGCYC	3 cut(s)	138, 549, 1339
ErhI	CCWWGG	2 cut(s)	349, 1339
FalI	AAGNNNNNCTT	4 cut(s)	242, 274, 752, 784
FaqI	GGGAC	3 cut(s)	397, 533, 1151
FauI	CCCGC	2 cut(s)	209, 307
FbaI	TGATCA	3 cut(s)	594, 1138, 1621
FokI	GGATG	5 cut(s)	77, 515, 924, 1721, 1769
FriOI	GRGCYC	3 cut(s)	138, 549, 1339
FspBI	CTAG	4 cut(s)	707, 1199, 1314, 1340
GlaI	GCGC	3 cut(s)	79, 181, 325
GsaI	CCCAGC	1 cut(s)	1617
GsuI	CTGGAG	2 cut(s)	38, 1167
HaeII	RGCGCY	1 cut(s)	327
HaeIII	GGCC	5 cut(s)	33, 58, 481, 653, 972
HapII	CCGG	5 cut(s)	482, 572, 849, 873, 1552
HgaI	GACGC	4 cut(s)	91, 193, 432, 1203
HhaI	GCGC	3 cut(s)	80, 182, 326
Hin1I	GRCGYC	1 cut(s)	443
Hin6I	GCGC	3 cut(s)	78, 180, 324
HinP1I	GCGC	3 cut(s)	78, 180, 324
HincII	GTYRAC	1 cut(s)	1095
HindII	GTYRAC	1 cut(s)	1095
HindIII	AAGCTT	1 cut(s)	566
HinfI	GANTC	5 cut(s)	464, 605, 823, 989, 1205
HpaI	GTTAAC	1 cut(s)	1095
HpaII	CCGG	5 cut(s)	482, 572, 849, 873, 1552
HphI	GGTGA	5 cut(s)	487, 1010, 1214, 1426, 1590
Hpy166II	GTNNAC	4 cut(s)	111, 870, 1095, 1598
Hpy8I	GTNNAC	4 cut(s)	111, 870, 1095, 1598
Hpy99I	CGWCG	5 cut(s)	209, 412, 436, 448, 451
HpyAV	CCTTC	5 cut(s)	225, 247, 268, 902, 1156
HpyCH4III	ACNGT	3 cut(s)	117, 1515, 1532
HpyCH4IV	ACGT	1 cut(s)	599
HpyF3I	CTNAG	5 cut(s)	98, 998, 1671, 1804, 1831
HpySE526I	ACGT	1 cut(s)	599
Hsp92I	GRCGYC	1 cut(s)	443
HspAI	GCGC	3 cut(s)	78, 180, 324
Kpn2I	TCCGGA	2 cut(s)	571, 1551
KpnI	GGTACC	1 cut(s)	1410
KroI	GCCGGC	1 cut(s)	481
KroNI	GCCGGC	1 cut(s)	483
Ksp22I	TGATCA	3 cut(s)	594, 1138, 1621
KspAI	GTTAAC	1 cut(s)	1095
LmnI	GCTCC	6 cut(s)	141, 239, 676, 875, 921, 1334
Lsp1109I	GCAGC	8 cut(s)	136, 877, 890, 1077, 1583, 1597, 1618, 1646
MaeI	CTAG	4 cut(s)	707, 1199, 1314, 1340
MaeII	ACGT	1 cut(s)	599
MaeIII	GTNAC	3 cut(s)	160, 1306, 1561
MboII	GAAGA	6 cut(s)	830, 937, 1288, 1349, 1453, 1590
MfeI	CAATTG	1 cut(s)	813
MflI	RGATCY	2 cut(s)	1058, 1548
MhlI	GDGCHC	7 cut(s)	113, 138, 535, 549, 647, 926, 1339
MluCI	AATT	9 cut(s)	49, 354, 361, 373, 382, 701, 813, 1223, 1605
MlyI	GAGTC	1 cut(s)	1214
MmeI	TCCRAC	4 cut(s)	300, 338, 1788, 1810
Mph1103I	ATGCAT	2 cut(s)	1105, 1451
MroI	TCCGGA	2 cut(s)	571, 1551
MroNI	GCCGGC	1 cut(s)	481
MroXI	GAANNNNTTC	2 cut(s)	257, 822
MseI	TTAA	7 cut(s)	537, 714, 753, 1094, 1238, 1323, 1821
MspA1I	CMGCKG	1 cut(s)	890
MspCI	CTTAAG	1 cut(s)	1820
MspI	CCGG	5 cut(s)	482, 572, 849, 873, 1552
MspR9I	CCNGG	7 cut(s)	46, 516, 849, 934, 975, 1494, 1651
MunI	CAATTG	1 cut(s)	813
Mva1269I	GAATGC	1 cut(s)	1502
MvaI	CCWGG	6 cut(s)	46, 516, 934, 975, 1494, 1651
MvnI	CGCG	3 cut(s)	180, 204, 618
NaeI	GCCGGC	1 cut(s)	483
NciI	CCSGG	1 cut(s)	849
NcoI	CCATGG	1 cut(s)	349
NgoMIV	GCCGGC	1 cut(s)	481
NlaIV	GGNNCC	5 cut(s)	931, 1167, 1336, 1408, 1550
NmeAIII	GCCGAG	3 cut(s)	157, 328, 1691
NmuCI	GTSAC	1 cut(s)	160
NsiI	ATGCAT	2 cut(s)	1105, 1451
NspI	RCATGY	1 cut(s)	1449
PaeR7I	CTCGAG	2 cut(s)	646, 1686
PagI	TCATGA	2 cut(s)	622, 1191
PcsI	WCGNNNNNNNCGW	8 cut(s)	204, 407, 416, 440, 452, 605, 644, 990
PctI	GAATGC	1 cut(s)	1502
PdiI	GCCGGC	1 cut(s)	483
PdmI	GAANNNNTTC	2 cut(s)	257, 822
PfeI	GAWTC	4 cut(s)	464, 605, 823, 989
PflFI	GACNNNGTC	1 cut(s)	158
PfoI	TCCNGGA	2 cut(s)	44, 932
Ple19I	CGATCG	1 cut(s)	27
PleI	GAGTC	1 cut(s)	1213
PpsI	GAGTC	1 cut(s)	1213
PpuMI	RGGWCCY	2 cut(s)	1165, 1343
Psp124BI	GAGCTC	2 cut(s)	138, 549
Psp5II	RGGWCCY	2 cut(s)	1165, 1343
Psp6I	CCWGG	6 cut(s)	44, 514, 932, 973, 1492, 1649
PspFI	CCCAGC	1 cut(s)	1613
PspGI	CCWGG	6 cut(s)	44, 514, 932, 973, 1492, 1649
PspN4I	GGNNCC	5 cut(s)	931, 1167, 1336, 1408, 1550
PspPI	GGNCC	7 cut(s)	31, 42, 651, 956, 971, 1165, 1343
PspPPI	RGGWCCY	2 cut(s)	1165, 1343
PspXI	VCTCGAGB	1 cut(s)	646
PstI	CTGCAG	2 cut(s)	1573, 1636
PsuI	RGATCY	2 cut(s)	1058, 1548
PsyI	GACNNNGTC	1 cut(s)	158
PvuI	CGATCG	1 cut(s)	27
PvuII	CAGCTG	1 cut(s)	890
RsaI	GTAC	4 cut(s)	335, 871, 1408, 1429
RsaNI	GTAC	4 cut(s)	334, 870, 1407, 1428
SacI	GAGCTC	2 cut(s)	138, 549
SaqAI	TTAA	7 cut(s)	537, 714, 753, 1094, 1238, 1323, 1821
Sau96I	GGNCC	7 cut(s)	31, 42, 651, 956, 971, 1165, 1343
SchI	GAGTC	1 cut(s)	1214
ScrFI	CCNGG	7 cut(s)	46, 516, 849, 934, 975, 1494, 1651
SduI	GDGCHC	7 cut(s)	113, 138, 535, 549, 647, 926, 1339
SfcI	CTRYAG	3 cut(s)	1051, 1569, 1632
Sfr274I	CTCGAG	2 cut(s)	646, 1686
SinI	GGWCC	4 cut(s)	42, 956, 1165, 1343
SlaI	CTCGAG	2 cut(s)	646, 1686
SmlI	CTYRAG	3 cut(s)	646, 1686, 1820
SmoI	CTYRAG	3 cut(s)	646, 1686, 1820
Sse9I	AATT	9 cut(s)	49, 354, 361, 373, 382, 701, 813, 1223, 1605
SspMI	CTAG	4 cut(s)	707, 1199, 1314, 1340
SstI	GAGCTC	2 cut(s)	138, 549
StyD4I	CCNGG	7 cut(s)	44, 514, 847, 932, 973, 1492, 1649
StyI	CCWWGG	2 cut(s)	349, 1339
TaaI	ACNGT	3 cut(s)	117, 1515, 1532
TaiI	ACGT	1 cut(s)	602
TasI	AATT	9 cut(s)	49, 354, 361, 373, 382, 701, 813, 1223, 1605
TauI	GCSGC	5 cut(s)	61, 83, 180, 440, 443
TfiI	GAWTC	4 cut(s)	464, 605, 823, 989
Tru1I	TTAA	7 cut(s)	537, 714, 753, 1094, 1238, 1323, 1821
Tru9I	TTAA	7 cut(s)	537, 714, 753, 1094, 1238, 1323, 1821
TscAI	CASTG	2 cut(s)	1393, 1647
TseFI	GTSAC	1 cut(s)	160
TseI	GCWGC	8 cut(s)	124, 878, 890, 1065, 1571, 1610, 1631, 1634
Tsp45I	GTSAC	1 cut(s)	160
TspDTI	ATGAA	6 cut(s)	42, 611, 815, 1180, 1507, 1799
TspRI	CASTG	2 cut(s)	1393, 1647
Tth111I	GACNNNGTC	1 cut(s)	158
Vha464I	CTTAAG	1 cut(s)	1820
VneI	GTGCAC	1 cut(s)	109
VpaK11BI	GGWCC	4 cut(s)	42, 956, 1165, 1343
XapI	RAATTY	3 cut(s)	49, 354, 373
XceI	RCATGY	1 cut(s)	1449
XhoI	CTCGAG	2 cut(s)	646, 1686
XmaJI	CCTAGG	1 cut(s)	1339
XmnI	GAANNNNTTC	2 cut(s)	257, 822
XspI	CTAG	4 cut(s)	707, 1199, 1314, 1340
Zsp2I	ATGCAT	2 cut(s)	1105, 1451

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.