Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr4

Orthogroup ID

OG0008336

Physical Location & Seq

Genomic Coordinates Forward (+)

20656533 .. 20657945

Gene Structure 1413 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom04g18740.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1413 bp

ATGGTATTCACATCTGGACCTGCAACTTTGGGCCCAGGAATAATCGTCGATTCAGATCTTAGCAAGTCCATCAGAACTCACCGAGACCTCATCAGTGGTCATACCCCTTACTATAGTAGATCTTCCAGCTTTTATAGGCAAGTGTCGCAGAGGTTAAGTGATGCATCTATTGTTCTCGATATGTTTGCTTGTTCTCTTGATCAAACTGGAACTGCAGAGCTGAAGGTCCCTGTTGAGAAGTCAGGTGGTTTCATGTTGCTAGGTGAGTCATTTGCTTCAAATCAATTCAGAAAATGTCTGAGCCACATTTTTACTCGTGATGAAGGGGGATATTTGAAGATGTATTTTGATGCAACTATTGAGATAGTGACCACTAAAGATATTAAAATCTGTGGAGCCCTAGGTCCTTGTGTATCTCTTCGTGAAGTCAACGATTTAGTGAGTAGCAGTGAGATTGGGGAGGGTGGTACCTATATGTGGAAGTTGGGTACAATCACCAATAAAACATGCATTGCTTTTTTATTCCAACTGAATGATGAGCAGAAAGTTCAACCTGGGTCGGCATTTTTGATACAGTTCATAACACGATACCGTCATGGGAACATGAGAATCCGGAAAAGGGTAACAACTGCAGCAAGAAGATGGGTTGGAGGCCGTTCACCAGAAATTGCTGCTGGGTTTGATCAAGAGGCAGCAGCTTCAGTGATGGCCAGGCTTGCTATTCACCGAGCTGAGAATTGTTTCGCTCGAGATGTTATCAGATGGCTGGATGACACATTGATCCGCTTTGCTTCCAAGTTTGGAGACTATGCAGAGGAAGATCCAACTTCCTTCCGCTTGTCATCCAACTTCTTCCTCTTTCCTCAGTTCATGTATTACTTGAGGAGGTCGCAGTTTATTGATGTCTTCAACAGTAGTCCTGATGAGACTGCTTTCTTCCGGCTGATGCTGAATCGCAAGGGGGTGGTGGGTTCTCTTATCATGATCCAACCCACGCTTTTCCAATACTCCTTTGATGGTCCACCCGTTCCAGTCCTCCTAGATGTCCGCTCCATCTCTCCAGATGTTATCTTGCTCTTTGACTCATATTTTCACGTGGTTATTCATTACGGGTCCAAAGTTTCTCAGTGGAGGAAGCTTGGGTATGACCAGGACCCAAACCATGAGAATTTGAGGAAACTGTTGGAAGCCCCGGAAATTGATGCAGAGCAGTTGATGGCTGACCGAGTTCCTGCACCAAAGTTTATTAAATGTGACCAGCATGGTAGCCAGGCAAGGTTTCTTCTTGCAAAGTTGAATCCATCTGTTACTCAGAATTCAACATACACTGACGGGTCAGATATTATTTTCACTGATGATCTGAGCTTGCAAGTATTTTTAGATCATTTGCAGGTCCTTGCAGTGCAAGGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001501 GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005798 GO:0005829 GO:0006810 GO:0006888 GO:0006900 GO:0006996 GO:0007275 GO:0008150 GO:0008270 GO:0009653 GO:0009790 GO:0009792 GO:0009887 GO:0009888 GO:0009987 GO:0012505 GO:0012506 GO:0012507 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0019898 GO:0022607 GO:0030117 GO:0030120 GO:0030127 GO:0030133 GO:0030134 GO:0030135 GO:0030658 GO:0030659 GO:0030660 GO:0030662 GO:0031090 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032991 GO:0033333 GO:0033334 GO:0033339 GO:0034622 GO:0035107 GO:0035138 GO:0035459 GO:0043009 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0046907 GO:0046914 GO:0048193 GO:0048194 GO:0048475 GO:0048513 GO:0048562 GO:0048568 GO:0048598 GO:0048701 GO:0048702 GO:0048704 GO:0048705 GO:0048706 GO:0048731 GO:0048736 GO:0048856 GO:0051179 GO:0051216 GO:0051234 GO:0051641 GO:0051649 GO:0051668 GO:0061024 GO:0061448 GO:0065003 GO:0070971 GO:0071840 GO:0090110 GO:0090114 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:1904888

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

Gelsolin Sec23_BS Sec23_helical Sec23_trunk zf-Sec23_Sec24

Protein Analysis

471

Amino Acids

52.95

Weight (kDa)

6.57

Isoelectric Point (pI)

45.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Sec23_trunk	PF04811	1 - 104	3.7e-16	Sec23/Sec24 trunk domain
Sec23_BS	PF08033	116 - 216	1.6e-19	Sec23/Sec24 beta-sandwich domain
Sec23_helical	PF04815	228 - 323	4.9e-24	Sec23/Sec24 helical domain
Gelsolin	PF00626	342 - 428	4.9e-09	Gelsolin repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008336)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G43670
fragaria_vesca	FvH4_3g28910 FvH4_3g28910 FvH4_3g28910
malus_domestica	MD04G1211400.v1.1 MD12G1226200.v1.1
prunus_persica	Prupe.1G027900_v2.0.a1
pyrus_communis	pycom04g01860 pycom04g18730 pycom04g18740 pycom12g21060 pycom12g21090
rosa_chinensis	RchiOBHm_Chr5g0051941
rosa_laevigata	RLG00000034852
rosa_multiflora	Rmu_co8514153.1_g000001
rosa_roxburghii	Rroxscaffold_1G00027500
rosa_rugosa	Rorug05G0276200 Rorug05G0276300
rosa_samantha	Rh5AG348200 Rh5BG358800 Rh5CG384400 Rh5DG374200
rosa_wichuraiana	Rw5G032860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	28, 1381
Acc65I	GGTACC	1 cut(s)	467
AccB1I	GGYRCC	1 cut(s)	467
AccBSI	CCGCTC	1 cut(s)	1050
AccIII	TCCGGA	1 cut(s)	612
AciI	CCGC	3 cut(s)	784, 835, 1048
AclWI	GGATC	3 cut(s)	775, 815, 979
AcoI	YGGCCR	1 cut(s)	708
AcsI	RAATTY	2 cut(s)	1168, 1315
AcuI	CTGAAG	2 cut(s)	242, 684
AcvI	CACGTG	1 cut(s)	1096
AfaI	GTAC	2 cut(s)	469, 490
AfiI	CCNNNNNNNGG	2 cut(s)	477, 619
AgsI	TTSAA	6 cut(s)	279, 337, 551, 910, 1297, 1320
AjnI	CCWGG	5 cut(s)	34, 553, 710, 1149, 1269
AluBI	AGCT	6 cut(s)	129, 220, 698, 731, 1138, 1365
AluI	AGCT	6 cut(s)	129, 220, 698, 731, 1138, 1365
Alw26I	GTCTC	3 cut(s)	78, 798, 920
AlwI	GGATC	3 cut(s)	775, 815, 979
Ama87I	CYCGRG	1 cut(s)	747
Aor13HI	TCCGGA	1 cut(s)	612
AoxI	GGCC	3 cut(s)	31, 652, 708
ApaI	GGGCCC	1 cut(s)	35
ApeKI	GCWGC	4 cut(s)	632, 671, 692, 695
ApoI	RAATTY	2 cut(s)	1168, 1315
Asp700I	GAANNNNTTC	1 cut(s)	740
Asp718I	GGTACC	1 cut(s)	467
AspA2I	CCTAGG	1 cut(s)	400
AspS9I	GGNCC	9 cut(s)	17, 31, 32, 226, 404, 1019, 1113, 1153, 1393
AsuC2I	CCSGG	1 cut(s)	1193
AsuHPI	GGTGA	5 cut(s)	71, 275, 487, 651, 716
AvaI	CYCGRG	1 cut(s)	747
AvaII	GGWCC	7 cut(s)	17, 226, 404, 1019, 1113, 1153, 1393
AvrII	CCTAGG	1 cut(s)	400
BaeGI	GKGCMC	1 cut(s)	35
BalI	TGGCCA	1 cut(s)	710
BanI	GGYRCC	1 cut(s)	467
BanII	GRGCYC	2 cut(s)	35, 400
BauI	CACGAG	1 cut(s)	315
BbrPI	CACGTG	1 cut(s)	1096
BbsI	GAAGAC	1 cut(s)	898
BbvI	GCAGC	4 cut(s)	644, 658, 704, 707
BccI	CCATC	8 cut(s)	77, 636, 700, 756, 1010, 1061, 1210, 1309
BceAI	ACGGC	1 cut(s)	639
BcgI	CGANNNNNNTGC	2 cut(s)	167, 201
BciT130I	CCWGG	5 cut(s)	36, 555, 712, 1151, 1271
BclI	TGATCA	2 cut(s)	199, 682
BcnI	CCSGG	1 cut(s)	1193
BcoDI	GTCTC	3 cut(s)	78, 798, 920
BfaI	CTAG	3 cut(s)	260, 401, 1040
BfmI	CTRYAG	3 cut(s)	112, 213, 630
BfuAI	ACCTGC	2 cut(s)	28, 1381
BglII	AGATCT	2 cut(s)	55, 119
BisI	GCNGC	4 cut(s)	633, 672, 693, 696
BlnI	CCTAGG	1 cut(s)	400
BlsI	GCNGC	4 cut(s)	634, 673, 694, 697
Bme1390I	CCNGG	6 cut(s)	36, 555, 712, 1151, 1193, 1271
Bme18I	GGWCC	7 cut(s)	17, 226, 404, 1019, 1113, 1153, 1393
BmeT110I	CYCGRG	1 cut(s)	747
BmgT120I	GGNCC	9 cut(s)	17, 31, 32, 226, 404, 1019, 1113, 1153, 1393
BmiI	GGNNCC	6 cut(s)	33, 228, 397, 469, 1114, 1155
BmrFI	CCNGG	6 cut(s)	36, 555, 712, 1151, 1193, 1271
BmsI	GCATC	5 cut(s)	151, 173, 340, 936, 1192
BpiI	GAAGAC	1 cut(s)	898
BpmI	CTGGAG	1 cut(s)	1044
BpuEI	CTTGAG	1 cut(s)	901
BpuMI	CCSGG	1 cut(s)	1193
BsaAI	YACGTR	1 cut(s)	1096
BsaBI	GATNNNNATC	1 cut(s)	54
BsaI	GGTCTC	1 cut(s)	78
BsaJI	CCNNGG	4 cut(s)	34, 400, 554, 1191
BsaWI	WCCGGW	1 cut(s)	612
Bsc4I	CCNNNNNNNGG	2 cut(s)	477, 619
Bse1I	ACTGG	2 cut(s)	211, 1031
Bse3DI	GCAATG	1 cut(s)	510
Bse8I	GATNNNNATC	1 cut(s)	54
BseAI	TCCGGA	1 cut(s)	612
BseBI	CCWGG	5 cut(s)	36, 555, 712, 1151, 1271
BseDI	CCNNGG	4 cut(s)	34, 400, 554, 1191
BseGI	GGATG	2 cut(s)	775, 842
BseJI	GATNNNNATC	1 cut(s)	54
BseLI	CCNNNNNNNGG	2 cut(s)	477, 619
BseMI	GCAATG	1 cut(s)	510
BseMII	CTCAG	6 cut(s)	290, 723, 878, 1139, 1325, 1352
BseNI	ACTGG	2 cut(s)	211, 1031
BseRI	GAGGAG	1 cut(s)	898
BseSI	GKGCMC	1 cut(s)	35
BseXI	GCAGC	4 cut(s)	644, 658, 704, 707
BseYI	CCCAGC	1 cut(s)	674
BsgI	GTGCAG	1 cut(s)	1218
BshFI	GGCC	3 cut(s)	33, 654, 710
BshNI	GGYRCC	1 cut(s)	467
BsiHKCI	CYCGRG	1 cut(s)	747
BsiSI	CCGG	3 cut(s)	613, 940, 1193
BslFI	GGGAC	1 cut(s)	212
BslI	CCNNNNNNNGG	2 cut(s)	477, 619
BsmAI	GTCTC	3 cut(s)	78, 798, 920
BsmFI	GGGAC	1 cut(s)	212
BsnI	GGCC	3 cut(s)	33, 654, 710
Bso31I	GGTCTC	1 cut(s)	78
BsoBI	CYCGRG	1 cut(s)	747
Bsp120I	GGGCCC	1 cut(s)	31
Bsp1286I	GDGCHC	2 cut(s)	35, 400
Bsp13I	TCCGGA	1 cut(s)	612
Bsp143I	GATC	9 cut(s)	55, 119, 199, 682, 780, 820, 984, 1357, 1381
BspACI	CCGC	3 cut(s)	784, 835, 1048
BspANI	GGCC	3 cut(s)	33, 654, 710
BspCNI	CTCAG	6 cut(s)	291, 724, 877, 1138, 1324, 1353
BspEI	TCCGGA	1 cut(s)	612
BspHI	TCATGA	1 cut(s)	981
BspLI	GGNNCC	6 cut(s)	33, 228, 397, 469, 1114, 1155
BspMAI	CTGCAG	2 cut(s)	217, 634
BspMI	ACCTGC	2 cut(s)	28, 1381
BspPI	GGATC	3 cut(s)	775, 815, 979
BspT107I	GGYRCC	1 cut(s)	467
BspTNI	GGTCTC	1 cut(s)	78
BsrBI	CCGCTC	1 cut(s)	1050
BsrDI	GCAATG	1 cut(s)	510
BsrI	ACTGG	2 cut(s)	211, 1031
BssECI	CCNNGG	4 cut(s)	34, 400, 554, 1191
BssMI	GATC	9 cut(s)	55, 119, 199, 682, 780, 820, 984, 1357, 1381
BssSI	CACGAG	1 cut(s)	315
BssT1I	CCWWGG	1 cut(s)	400
Bst2BI	CACGAG	1 cut(s)	315
Bst2UI	CCWGG	5 cut(s)	36, 555, 712, 1151, 1271
Bst4CI	ACNGT	4 cut(s)	576, 593, 914, 1182
Bst6I	CTCTTC	1 cut(s)	423
BstBAI	YACGTR	1 cut(s)	1096
BstC8I	GCNNGC	2 cut(s)	717, 1367
BstDEI	CTNAG	7 cut(s)	59, 299, 732, 864, 1125, 1311, 1361
BstF5I	GGATG	2 cut(s)	775, 842
BstKTI	GATC	9 cut(s)	58, 122, 202, 685, 783, 823, 987, 1360, 1384
BstMAI	GTCTC	3 cut(s)	78, 798, 920
BstMBI	GATC	9 cut(s)	55, 119, 199, 682, 780, 820, 984, 1357, 1381
BstMWI	GCNNNNNNNGC	2 cut(s)	145, 716
BstNI	CCWGG	5 cut(s)	36, 555, 712, 1151, 1271
BstNSI	RCATGY	1 cut(s)	510
BstSCI	CCNGG	6 cut(s)	34, 553, 710, 1149, 1191, 1269
BstSFI	CTRYAG	3 cut(s)	112, 213, 630
BstSLI	GKGCMC	1 cut(s)	35
BstV1I	GCAGC	4 cut(s)	644, 658, 704, 707
BstV2I	GAAGAC	1 cut(s)	898
BstX2I	RGATCY	3 cut(s)	55, 119, 820
BstYI	RGATCY	3 cut(s)	55, 119, 820
BsuRI	GGCC	3 cut(s)	33, 654, 710
BtsCI	GGATG	2 cut(s)	775, 842
BtsI	GCAGTG	2 cut(s)	454, 1407
BtsIMutI	CAGTG	7 cut(s)	100, 454, 708, 1133, 1326, 1350, 1407
BveI	ACCTGC	2 cut(s)	28, 1381
Cac8I	GCNNGC	2 cut(s)	717, 1367
CciI	TCATGA	1 cut(s)	981
Cfr13I	GGNCC	9 cut(s)	17, 31, 32, 226, 404, 1019, 1113, 1153, 1393
Csp6I	GTAC	2 cut(s)	468, 489
CviAII	CATG	8 cut(s)	253, 507, 596, 604, 871, 982, 1163, 1262
CviQI	GTAC	2 cut(s)	468, 489
DdeI	CTNAG	7 cut(s)	59, 299, 732, 864, 1125, 1311, 1361
DpnI	GATC	9 cut(s)	57, 121, 201, 684, 782, 822, 986, 1359, 1383
DpnII	GATC	9 cut(s)	55, 119, 199, 682, 780, 820, 984, 1357, 1381
EaeI	YGGCCR	1 cut(s)	708
Eam1104I	CTCTTC	1 cut(s)	423
EarI	CTCTTC	1 cut(s)	423
Eco130I	CCWWGG	1 cut(s)	400
Eco24I	GRGCYC	2 cut(s)	35, 400
Eco31I	GGTCTC	1 cut(s)	78
Eco47I	GGWCC	7 cut(s)	17, 226, 404, 1019, 1113, 1153, 1393
Eco57I	CTGAAG	2 cut(s)	242, 684
Eco72I	CACGTG	1 cut(s)	1096
Eco88I	CYCGRG	1 cut(s)	747
EcoO109I	RGGNCCY	4 cut(s)	226, 404, 1153, 1393
EcoRI	GAATTC	1 cut(s)	1315
EcoRII	CCWGG	5 cut(s)	34, 553, 710, 1149, 1269
EcoT14I	CCWWGG	1 cut(s)	400
EcoT22I	ATGCAT	2 cut(s)	166, 512
EcoT38I	GRGCYC	2 cut(s)	35, 400
ErhI	CCWWGG	1 cut(s)	400
FaeI	CATG	8 cut(s)	256, 510, 599, 607, 874, 985, 1166, 1265
FaqI	GGGAC	1 cut(s)	212
FatI	CATG	8 cut(s)	252, 506, 595, 603, 870, 981, 1162, 1261
FbaI	TGATCA	2 cut(s)	199, 682
Fnu4HI	GCNGC	4 cut(s)	633, 672, 693, 696
FokI	GGATG	2 cut(s)	782, 829
FriOI	GRGCYC	2 cut(s)	35, 400
Fsp4HI	GCNGC	4 cut(s)	633, 672, 693, 696
FspBI	CTAG	3 cut(s)	260, 401, 1040
GluI	GCNGC	4 cut(s)	633, 672, 693, 696
GsaI	CCCAGC	1 cut(s)	678
GsuI	CTGGAG	1 cut(s)	1044
HaeIII	GGCC	3 cut(s)	33, 654, 710
HapII	CCGG	3 cut(s)	613, 940, 1193
Hin1II	CATG	8 cut(s)	256, 510, 599, 607, 874, 985, 1166, 1265
HincII	GTYRAC	1 cut(s)	430
HindII	GTYRAC	1 cut(s)	430
HindIII	AAGCTT	1 cut(s)	1136
HinfI	GANTC	6 cut(s)	50, 266, 609, 952, 1082, 1297
HpaII	CCGG	3 cut(s)	613, 940, 1193
HphI	GGTGA	5 cut(s)	71, 275, 487, 651, 716
Hpy166II	GTNNAC	3 cut(s)	430, 659, 1022
Hpy188I	TCNGA	8 cut(s)	55, 74, 290, 300, 761, 1314, 1339, 1362
Hpy8I	GTNNAC	3 cut(s)	430, 659, 1022
Hpy99I	CGWCG	1 cut(s)	50
HpyAV	CCTTC	3 cut(s)	217, 317, 841
HpyCH4III	ACNGT	4 cut(s)	576, 593, 914, 1182
HpyCH4IV	ACGT	1 cut(s)	1095
HpyF10VI	GCNNNNNNNGC	2 cut(s)	145, 716
HpyF3I	CTNAG	7 cut(s)	59, 299, 732, 864, 1125, 1311, 1361
HpySE526I	ACGT	1 cut(s)	1095
Hsp92II	CATG	8 cut(s)	256, 510, 599, 607, 874, 985, 1166, 1265
Kpn2I	TCCGGA	1 cut(s)	612
KpnI	GGTACC	1 cut(s)	471
Ksp22I	TGATCA	2 cut(s)	199, 682
Kzo9I	GATC	9 cut(s)	55, 119, 199, 682, 780, 820, 984, 1357, 1381
LmnI	GCTCC	2 cut(s)	395, 1055
Lsp1109I	GCAGC	4 cut(s)	644, 658, 704, 707
LweI	GCATC	5 cut(s)	151, 173, 340, 936, 1192
MaeI	CTAG	3 cut(s)	260, 401, 1040
MaeII	ACGT	1 cut(s)	1095
MaeIII	GTNAC	4 cut(s)	367, 622, 1253, 1306
MalI	GATC	9 cut(s)	57, 121, 201, 684, 782, 822, 986, 1359, 1383
MbiI	CCGCTC	1 cut(s)	1050
MboI	GATC	9 cut(s)	55, 119, 199, 682, 780, 820, 984, 1357, 1381
MboII	GAAGA	9 cut(s)	114, 349, 410, 651, 830, 844, 898, 928, 1274
MflI	RGATCY	3 cut(s)	55, 119, 820
MhlI	GDGCHC	2 cut(s)	35, 400
MlsI	TGGCCA	1 cut(s)	710
MluCI	AATT	6 cut(s)	284, 666, 736, 1168, 1197, 1315
MluNI	TGGCCA	1 cut(s)	710
MlyI	GAGTC	2 cut(s)	275, 1076
MmeI	TCCRAC	6 cut(s)	550, 628, 848, 870, 1012, 1164
Mox20I	TGGCCA	1 cut(s)	710
Mph1103I	ATGCAT	2 cut(s)	166, 512
MroI	TCCGGA	1 cut(s)	612
MroXI	GAANNNNTTC	1 cut(s)	740
MscI	TGGCCA	1 cut(s)	710
MseI	TTAA	3 cut(s)	155, 384, 1248
Msp20I	TGGCCA	1 cut(s)	710
MspI	CCGG	3 cut(s)	613, 940, 1193
MspR9I	CCNGG	6 cut(s)	36, 555, 712, 1151, 1193, 1271
MvaI	CCWGG	5 cut(s)	36, 555, 712, 1151, 1271
MwoI	GCNNNNNNNGC	2 cut(s)	145, 716
NciI	CCSGG	1 cut(s)	1193
NdeII	GATC	9 cut(s)	55, 119, 199, 682, 780, 820, 984, 1357, 1381
NlaIII	CATG	8 cut(s)	256, 510, 599, 607, 874, 985, 1166, 1265
NlaIV	GGNNCC	6 cut(s)	33, 228, 397, 469, 1114, 1155
NmuCI	GTSAC	2 cut(s)	367, 1253
NsiI	ATGCAT	2 cut(s)	166, 512
NspI	RCATGY	1 cut(s)	510
PaeR7I	CTCGAG	1 cut(s)	747
PagI	TCATGA	1 cut(s)	981
PdmI	GAANNNNTTC	1 cut(s)	740
PfeI	GAWTC	4 cut(s)	50, 609, 952, 1297
PkrI	GCNGC	4 cut(s)	634, 673, 694, 697
PleI	GAGTC	2 cut(s)	274, 1076
PmaCI	CACGTG	1 cut(s)	1096
PmlI	CACGTG	1 cut(s)	1096
PpsI	GAGTC	2 cut(s)	274, 1076
Ppu21I	YACGTR	1 cut(s)	1096
PpuMI	RGGWCCY	4 cut(s)	226, 404, 1153, 1393
Psp5II	RGGWCCY	4 cut(s)	226, 404, 1153, 1393
Psp6I	CCWGG	5 cut(s)	34, 553, 710, 1149, 1269
PspCI	CACGTG	1 cut(s)	1096
PspFI	CCCAGC	1 cut(s)	674
PspGI	CCWGG	5 cut(s)	34, 553, 710, 1149, 1269
PspN4I	GGNNCC	6 cut(s)	33, 228, 397, 469, 1114, 1155
PspOMI	GGGCCC	1 cut(s)	31
PspPI	GGNCC	9 cut(s)	17, 31, 32, 226, 404, 1019, 1113, 1153, 1393
PspPPI	RGGWCCY	4 cut(s)	226, 404, 1153, 1393
PstI	CTGCAG	2 cut(s)	217, 634
PsuI	RGATCY	3 cut(s)	55, 119, 820
RsaI	GTAC	2 cut(s)	469, 490
RsaNI	GTAC	2 cut(s)	468, 489
SaqAI	TTAA	3 cut(s)	155, 384, 1248
SatI	GCNGC	4 cut(s)	633, 672, 693, 696
Sau3AI	GATC	9 cut(s)	55, 119, 199, 682, 780, 820, 984, 1357, 1381
Sau96I	GGNCC	9 cut(s)	17, 31, 32, 226, 404, 1019, 1113, 1153, 1393
SchI	GAGTC	2 cut(s)	275, 1076
ScrFI	CCNGG	6 cut(s)	36, 555, 712, 1151, 1193, 1271
SduI	GDGCHC	2 cut(s)	35, 400
SfaNI	GCATC	5 cut(s)	151, 173, 340, 936, 1192
SfcI	CTRYAG	3 cut(s)	112, 213, 630
Sfr274I	CTCGAG	1 cut(s)	747
SinI	GGWCC	7 cut(s)	17, 226, 404, 1019, 1113, 1153, 1393
SlaI	CTCGAG	1 cut(s)	747
SmlI	CTYRAG	2 cut(s)	747, 880
SmoI	CTYRAG	2 cut(s)	747, 880
Sse9I	AATT	6 cut(s)	284, 666, 736, 1168, 1197, 1315
SsiI	CCGC	3 cut(s)	784, 835, 1048
SspMI	CTAG	3 cut(s)	260, 401, 1040
StyD4I	CCNGG	6 cut(s)	34, 553, 710, 1149, 1191, 1269
StyI	CCWWGG	1 cut(s)	400
TaaI	ACNGT	4 cut(s)	576, 593, 914, 1182
TaiI	ACGT	1 cut(s)	1098
TaqI	TCGA	3 cut(s)	48, 177, 748
TaqII	GACCGA	1 cut(s)	1239
TasI	AATT	6 cut(s)	284, 666, 736, 1168, 1197, 1315
TfiI	GAWTC	4 cut(s)	50, 609, 952, 1297
Tru1I	TTAA	3 cut(s)	155, 384, 1248
Tru9I	TTAA	3 cut(s)	155, 384, 1248
TscAI	CASTG	7 cut(s)	100, 454, 708, 1133, 1333, 1357, 1407
TseFI	GTSAC	2 cut(s)	367, 1253
TseI	GCWGC	4 cut(s)	632, 671, 692, 695
Tsp45I	GTSAC	2 cut(s)	367, 1253
TspDTI	ATGAA	5 cut(s)	241, 336, 568, 859, 1094
TspRI	CASTG	7 cut(s)	100, 454, 708, 1133, 1333, 1357, 1407
VpaK11BI	GGWCC	7 cut(s)	17, 226, 404, 1019, 1113, 1153, 1393
XapI	RAATTY	2 cut(s)	1168, 1315
XceI	RCATGY	1 cut(s)	510
XhoI	CTCGAG	1 cut(s)	747
XmaJI	CCTAGG	1 cut(s)	400
XmnI	GAANNNNTTC	1 cut(s)	740
XspI	CTAG	3 cut(s)	260, 401, 1040
Zsp2I	ATGCAT	2 cut(s)	166, 512

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.