Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0009868

Physical Location & Seq

Genomic Coordinates Reverse (-)

29670552 .. 29673106

Gene Structure 2555 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1212400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1035 bp

ATGTCTGGGGGCAATGAAGTCAACGTTAGTGAATCCAGGATGGTGGTTCCATTGAATACATGGGTCCTCATCTCCAATTTTAAGCTGTCATACAATCTTCTGCGCCGCCCTGACGGGACCTTCAACCGCCACTTGGCAGAATTCCTTGACCGGAAAGTGCCAGCCAATTCTAACCCTGTTGATGGGGTTGTCTCGTTTGATGTCATCATCGACCGTGAAACTAGCCTGCTCAGTCGAATCTATCATCCGGACGATGCTAATCTATCCCCGCTGAACATTGTTGATCCTGAGAGAGCGGTGAGCCAAGAGGTTCTTCCTGTCATAGTTTTCTTCCATGGTGGAAGCTTTGCACACTCCTCTTCAAACAGCGGTATATATGATATTCTGTGTCGCCGACTAGTTGGTATCTGCAAGGCTGTAGTGGTCTCTGTGAATTATCGTAGGGCTCCTGAAAATCGCTATCCATGTGCCTATGATGATGGATGGACAGCCCTGAGGTGGGTGAACTCTAGATCATGGCTTAAAAGTACAAGGGACTCAAACGTTCATATCTATCTAGCTGGCGATAGCTCTGGCGGGAACATTGTACACAATGTTGCTTTAAGAGCAGCAGAATCTGGAATCAATGTACTGGGAAATATACTGCTCAACCCTATGTTTGGTGGGCAGGAGCGGACTGAATCCGAGTTGCGATTGGATGGGAAATACTTTGTAACCATCCAAGACCGGGACTGGTACTGGAGAGCTTTTCTCCCTGATGGTGAAGATAGGGACCATCCAGCATGTAACCCATTTGGTCCCCGTGGTCAAAGCCTCGAAGCTGTCAAGTTCCCGAAGAGCCTTGTTGTGGTGGCTGGTTTGGATCTTGTTCAGGACTGGCAATTGGCTTACGCCAGAGGGCTCGAGAGTGCTGGCAAAAACATCAAACTTATGTATCTTGAGCAGGCCACAATTGGTTTCTACCTGTTGCCGAATAATGAGCATTTCTACACCGTGATGGATGAGATAAGTAAATTCGTGCTCTCCGATAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005975 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0008289 GO:0009056 GO:0009058 GO:0009311 GO:0009312 GO:0009653 GO:0009719 GO:0009725 GO:0009739 GO:0009755 GO:0009791 GO:0009892 GO:0009908 GO:0009937 GO:0009939 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010154 GO:0010325 GO:0010331 GO:0010468 GO:0010476 GO:0010605 GO:0010629 GO:0010646 GO:0010647 GO:0016051 GO:0016787 GO:0019222 GO:0019840 GO:0022414 GO:0023051 GO:0023052 GO:0023056 GO:0031406 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0036094 GO:0042221 GO:0042562 GO:0043167 GO:0043168 GO:0043177 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043900 GO:0043902 GO:0044238 GO:0044424 GO:0044464 GO:0048367 GO:0048437 GO:0048444 GO:0048518 GO:0048519 GO:0048522 GO:0048530 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071495 GO:0071704 GO:0080154 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:1901576 GO:1901700 GO:1901701 GO:1905392 GO:1905516 GO:2000241 GO:2000243

KEGG Pathways

Metabolic & Signaling

ko04075 map04075

Pfam Domains

Protein Families

Abhydrolase_3

Protein Analysis

345

Amino Acids

38.63

Weight (kDa)

5.78

Isoelectric Point (pI)

49.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
COesterase	PF00135	91 - 149	3.4e-07	Carboxylesterase family
BD-FAE	PF20434	101 - 205	1.2e-09	BD-FAE
Abhydrolase_3	PF07859	108 - 321	6.2e-58	alpha/beta hydrolase fold

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009868)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05120 AT5G27320
fragaria_vesca	FvH4_6g04960 FvH4_6g04960 FvH4_6g04960 FvH4_6g04960
malus_domestica	MD04G1212400.v1.1 MD07G1046000.v1.1 MD12G1227200.v1.1
prunus_persica	Prupe.6G332800_v2.0.a1
pyrus_communis	pycom04g18850
rosa_chinensis	RchiOBHm_Chr3g0453451
rosa_laevigata	RLG00000025513
rosa_multiflora	Rmu_sc0000510.1_g000008
rosa_roxburghii	Rroxscaffold_6G00430420
rosa_rugosa	Rorug02G0649200
rosa_samantha	Rh3AG051900 Rh3BG053400 Rh3CG052700 Rh3DG053500
rosa_wichuraiana	Rw3G004000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	2 cut(s)	296, 673
AccIII	TCCGGA	1 cut(s)	247
AciI	CCGC	7 cut(s)	106, 127, 269, 296, 369, 576, 673
AclI	AACGTT	2 cut(s)	24, 543
AclWI	GGATC	2 cut(s)	278, 870
AcsI	RAATTY	2 cut(s)	140, 1013
AfaI	GTAC	4 cut(s)	529, 588, 630, 737
AfiI	CCNNNNNNNGG	7 cut(s)	133, 182, 498, 499, 659, 727, 847
AgsI	TTSAA	3 cut(s)	55, 124, 363
AhlI	ACTAGT	1 cut(s)	397
AjnI	CCWGG	1 cut(s)	35
AjuI	GAANNNNNNNTTGG	2 cut(s)	297, 329
AluBI	AGCT	6 cut(s)	85, 345, 560, 570, 746, 821
AluI	AGCT	6 cut(s)	85, 345, 560, 570, 746, 821
Alw21I	GWGCWC	1 cut(s)	1023
Alw26I	GTCTC	2 cut(s)	196, 430
AlwI	GGATC	2 cut(s)	278, 870
AlwNI	CAGNNNCTG	1 cut(s)	617
Ama87I	CYCGRG	1 cut(s)	902
Aor13HI	TCCGGA	1 cut(s)	247
AoxI	GGCC	1 cut(s)	945
ApeKI	GCWGC	1 cut(s)	608
ApoI	RAATTY	2 cut(s)	140, 1013
AspLEI	GCGC	1 cut(s)	105
AspS9I	GGNCC	4 cut(s)	64, 117, 772, 797
AsuC2I	CCSGG	1 cut(s)	728
AsuHPI	GGTGA	3 cut(s)	310, 514, 773
AvaI	CYCGRG	1 cut(s)	902
AvaII	GGWCC	4 cut(s)	64, 117, 772, 797
AxyI	CCTNAGG	1 cut(s)	494
BaeI	ACNNNNGTAYC	2 cut(s)	917, 950
BanII	GRGCYC	2 cut(s)	448, 903
Bbv12I	GWGCWC	1 cut(s)	1023
BbvI	GCAGC	1 cut(s)	620
BccI	CCATC	9 cut(s)	34, 176, 473, 477, 692, 725, 752, 783, 991
BciT130I	CCWGG	1 cut(s)	37
BcnI	CCSGG	1 cut(s)	728
BcoDI	GTCTC	2 cut(s)	196, 430
BcuI	ACTAGT	1 cut(s)	397
BfaI	CTAG	4 cut(s)	222, 398, 510, 557
BfmI	CTRYAG	1 cut(s)	417
BisI	GCNGC	2 cut(s)	106, 609
BlsI	GCNGC	2 cut(s)	107, 610
Bme1390I	CCNGG	2 cut(s)	37, 728
Bme18I	GGWCC	4 cut(s)	64, 117, 772, 797
BmeT110I	CYCGRG	1 cut(s)	902
BmgT120I	GGNCC	4 cut(s)	64, 117, 772, 797
BmiI	GGNNCC	6 cut(s)	48, 65, 118, 447, 773, 799
BmrFI	CCNGG	2 cut(s)	37, 728
BmrI	ACTGGG	1 cut(s)	641
BmsI	GCATC	1 cut(s)	244
BmuI	ACTGGG	1 cut(s)	641
BplI	GAGNNNNNCTC	2 cut(s)	735, 767
BpmI	CTGGAG	1 cut(s)	760
BpuEI	CTTGAG	1 cut(s)	959
BpuMI	CCSGG	1 cut(s)	728
BsaBI	GATNNNNATC	1 cut(s)	258
BsaI	GGTCTC	1 cut(s)	430
BsaJI	CCNNGG	2 cut(s)	334, 802
BsaWI	WCCGGW	2 cut(s)	150, 247
Bsc4I	CCNNNNNNNGG	7 cut(s)	133, 182, 498, 499, 659, 727, 847
Bse1I	ACTGG	4 cut(s)	636, 737, 743, 881
Bse21I	CCTNAGG	1 cut(s)	494
Bse3DI	GCAATG	1 cut(s)	19
Bse8I	GATNNNNATC	1 cut(s)	258
BseAI	TCCGGA	1 cut(s)	247
BseBI	CCWGG	1 cut(s)	37
BseDI	CCNNGG	2 cut(s)	334, 802
BseGI	GGATG	7 cut(s)	45, 244, 488, 703, 717, 775, 1006
BseJI	GATNNNNATC	1 cut(s)	258
BseLI	CCNNNNNNNGG	7 cut(s)	133, 182, 498, 499, 659, 727, 847
BseMI	GCAATG	1 cut(s)	19
BseMII	CTCAG	3 cut(s)	244, 279, 485
BseNI	ACTGG	4 cut(s)	636, 737, 743, 881
BseRI	GAGGAG	1 cut(s)	346
BseXI	GCAGC	1 cut(s)	620
Bsh1285I	CGRYCG	1 cut(s)	214
BshFI	GGCC	1 cut(s)	947
BsiEI	CGRYCG	1 cut(s)	214
BsiHKAI	GWGCWC	1 cut(s)	1023
BsiHKCI	CYCGRG	1 cut(s)	902
BsiSI	CCGG	3 cut(s)	151, 248, 727
BslFI	GGGAC	5 cut(s)	130, 548, 743, 783, 785
BslI	CCNNNNNNNGG	7 cut(s)	133, 182, 498, 499, 659, 727, 847
BsmAI	GTCTC	2 cut(s)	196, 430
BsmFI	GGGAC	5 cut(s)	130, 548, 743, 783, 785
BsnI	GGCC	1 cut(s)	947
Bso31I	GGTCTC	1 cut(s)	430
BsoBI	CYCGRG	1 cut(s)	902
Bsp1286I	GDGCHC	3 cut(s)	448, 903, 1023
Bsp13I	TCCGGA	1 cut(s)	247
Bsp1407I	TGTACA	1 cut(s)	586
Bsp143I	GATC	3 cut(s)	283, 512, 862
Bsp19I	CCATGG	1 cut(s)	334
BspACI	CCGC	7 cut(s)	106, 127, 269, 296, 369, 576, 673
BspANI	GGCC	1 cut(s)	947
BspCNI	CTCAG	3 cut(s)	243, 280, 486
BspEI	TCCGGA	1 cut(s)	247
BspLI	GGNNCC	6 cut(s)	48, 65, 118, 447, 773, 799
BspPI	GGATC	2 cut(s)	278, 870
BspQI	GCTCTTC	1 cut(s)	830
BspTNI	GGTCTC	1 cut(s)	430
BsrBI	CCGCTC	2 cut(s)	296, 673
BsrDI	GCAATG	1 cut(s)	19
BsrGI	TGTACA	1 cut(s)	586
BsrI	ACTGG	4 cut(s)	636, 737, 743, 881
BssECI	CCNNGG	2 cut(s)	334, 802
BssMI	GATC	3 cut(s)	283, 512, 862
BssT1I	CCWWGG	1 cut(s)	334
Bst2UI	CCWGG	1 cut(s)	37
Bst4CI	ACNGT	2 cut(s)	215, 994
Bst6I	CTCTTC	2 cut(s)	364, 830
BstAUI	TGTACA	1 cut(s)	586
BstC8I	GCNNGC	5 cut(s)	162, 227, 562, 913, 945
BstDEI	CTNAG	3 cut(s)	230, 288, 494
BstDSI	CCRYGG	2 cut(s)	334, 802
BstF5I	GGATG	7 cut(s)	45, 244, 488, 703, 717, 775, 1006
BstHHI	GCGC	1 cut(s)	105
BstKTI	GATC	3 cut(s)	286, 515, 865
BstMAI	GTCTC	2 cut(s)	196, 430
BstMBI	GATC	3 cut(s)	283, 512, 862
BstMCI	CGRYCG	1 cut(s)	214
BstMWI	GCNNNNNNNGC	1 cut(s)	605
BstNI	CCWGG	1 cut(s)	37
BstNSI	RCATGY	1 cut(s)	786
BstSCI	CCNGG	2 cut(s)	35, 726
BstSFI	CTRYAG	1 cut(s)	417
BstV1I	GCAGC	1 cut(s)	620
BstX2I	RGATCY	1 cut(s)	862
BstXI	CCANNNNNNTGG	1 cut(s)	43
BstYI	RGATCY	1 cut(s)	862
Bsu36I	CCTNAGG	1 cut(s)	494
BsuRI	GGCC	1 cut(s)	947
BtgI	CCRYGG	2 cut(s)	334, 802
BtsCI	GGATG	7 cut(s)	45, 244, 488, 703, 717, 775, 1006
Cac8I	GCNNGC	5 cut(s)	162, 227, 562, 913, 945
CaiI	CAGNNNCTG	1 cut(s)	617
CfoI	GCGC	1 cut(s)	105
Cfr13I	GGNCC	4 cut(s)	64, 117, 772, 797
Csp6I	GTAC	4 cut(s)	528, 587, 629, 736
CviAII	CATG	5 cut(s)	60, 335, 465, 516, 783
CviQI	GTAC	4 cut(s)	528, 587, 629, 736
DdeI	CTNAG	3 cut(s)	230, 288, 494
DpnI	GATC	3 cut(s)	285, 514, 864
DpnII	GATC	3 cut(s)	283, 512, 862
Eam1104I	CTCTTC	2 cut(s)	364, 830
EarI	CTCTTC	2 cut(s)	364, 830
Eco130I	CCWWGG	1 cut(s)	334
Eco24I	GRGCYC	2 cut(s)	448, 903
Eco31I	GGTCTC	1 cut(s)	430
Eco47I	GGWCC	4 cut(s)	64, 117, 772, 797
Eco81I	CCTNAGG	1 cut(s)	494
Eco88I	CYCGRG	1 cut(s)	902
EcoO109I	RGGNCCY	2 cut(s)	64, 117
EcoRI	GAATTC	1 cut(s)	140
EcoRII	CCWGG	1 cut(s)	35
EcoT14I	CCWWGG	1 cut(s)	334
EcoT38I	GRGCYC	2 cut(s)	448, 903
ErhI	CCWWGG	1 cut(s)	334
FaeI	CATG	5 cut(s)	63, 338, 468, 519, 786
FalI	AAGNNNNNCTT	2 cut(s)	297, 329
FaqI	GGGAC	5 cut(s)	130, 548, 743, 783, 785
FatI	CATG	5 cut(s)	59, 334, 464, 515, 782
FauI	CCCGC	2 cut(s)	276, 569
Fnu4HI	GCNGC	2 cut(s)	106, 609
FokI	GGATG	7 cut(s)	52, 231, 495, 704, 710, 762, 1013
FriOI	GRGCYC	2 cut(s)	448, 903
Fsp4HI	GCNGC	2 cut(s)	106, 609
FspBI	CTAG	4 cut(s)	222, 398, 510, 557
GlaI	GCGC	1 cut(s)	104
GluI	GCNGC	2 cut(s)	106, 609
GsuI	CTGGAG	1 cut(s)	760
HaeIII	GGCC	1 cut(s)	947
HapII	CCGG	3 cut(s)	151, 248, 727
HhaI	GCGC	1 cut(s)	105
Hin1II	CATG	5 cut(s)	63, 338, 468, 519, 786
Hin6I	GCGC	1 cut(s)	103
HinP1I	GCGC	1 cut(s)	103
HincII	GTYRAC	1 cut(s)	22
HindII	GTYRAC	1 cut(s)	22
HindIII	AAGCTT	1 cut(s)	343
HinfI	GANTC	6 cut(s)	32, 237, 536, 614, 621, 680
HpaII	CCGG	3 cut(s)	151, 248, 727
HphI	GGTGA	3 cut(s)	310, 514, 773
Hpy166II	GTNNAC	3 cut(s)	22, 505, 589
Hpy188I	TCNGA	2 cut(s)	685, 1027
Hpy188III	TCNNGA	9 cut(s)	248, 287, 449, 510, 618, 832, 872, 904, 938
Hpy8I	GTNNAC	3 cut(s)	22, 505, 589
HpyAV	CCTTC	1 cut(s)	130
HpyCH4III	ACNGT	2 cut(s)	215, 994
HpyCH4IV	ACGT	2 cut(s)	24, 543
HpyCH4V	TGCA	2 cut(s)	350, 411
HpyF10VI	GCNNNNNNNGC	1 cut(s)	605
HpyF3I	CTNAG	3 cut(s)	230, 288, 494
HpySE526I	ACGT	2 cut(s)	24, 543
Hsp92II	CATG	5 cut(s)	63, 338, 468, 519, 786
HspAI	GCGC	1 cut(s)	103
Kpn2I	TCCGGA	1 cut(s)	247
Kzo9I	GATC	3 cut(s)	283, 512, 862
LguI	GCTCTTC	1 cut(s)	830
LmnI	GCTCC	2 cut(s)	451, 670
Lsp1109I	GCAGC	1 cut(s)	620
LweI	GCATC	1 cut(s)	244
MaeI	CTAG	4 cut(s)	222, 398, 510, 557
MaeII	ACGT	2 cut(s)	24, 543
MaeIII	GTNAC	2 cut(s)	712, 785
MalI	GATC	3 cut(s)	285, 514, 864
MbiI	CCGCTC	2 cut(s)	296, 673
MboI	GATC	3 cut(s)	283, 512, 862
MboII	GAAGA	6 cut(s)	89, 305, 322, 351, 776, 847
MfeI	CAATTG	2 cut(s)	881, 951
MflI	RGATCY	1 cut(s)	862
MhlI	GDGCHC	3 cut(s)	448, 903, 1023
MluCI	AATT	8 cut(s)	76, 140, 166, 433, 881, 951, 1013, 1030
MlyI	GAGTC	1 cut(s)	530
MnlI	CCTC	6 cut(s)	77, 301, 367, 489, 824, 890
MroI	TCCGGA	1 cut(s)	247
MseI	TTAA	3 cut(s)	81, 522, 602
MslI	CAYNNNNRTG	1 cut(s)	995
MspA1I	CMGCKG	2 cut(s)	271, 369
MspI	CCGG	3 cut(s)	151, 248, 727
MspR9I	CCNGG	2 cut(s)	37, 728
MunI	CAATTG	2 cut(s)	881, 951
MvaI	CCWGG	1 cut(s)	37
MwoI	GCNNNNNNNGC	1 cut(s)	605
NciI	CCSGG	1 cut(s)	728
NcoI	CCATGG	1 cut(s)	334
NdeII	GATC	3 cut(s)	283, 512, 862
NlaIII	CATG	5 cut(s)	63, 338, 468, 519, 786
NlaIV	GGNNCC	6 cut(s)	48, 65, 118, 447, 773, 799
NspI	RCATGY	1 cut(s)	786
PaeR7I	CTCGAG	1 cut(s)	902
PciSI	GCTCTTC	1 cut(s)	830
PcsI	WCGNNNNNNNCGW	1 cut(s)	1023
PfeI	GAWTC	5 cut(s)	32, 237, 614, 621, 680
PfoI	TCCNGGA	1 cut(s)	35
PkrI	GCNGC	2 cut(s)	107, 610
PleI	GAGTC	1 cut(s)	530
PpsI	GAGTC	1 cut(s)	530
PpuMI	RGGWCCY	2 cut(s)	64, 117
Psp1406I	AACGTT	2 cut(s)	24, 543
Psp5II	RGGWCCY	2 cut(s)	64, 117
Psp6I	CCWGG	1 cut(s)	35
PspGI	CCWGG	1 cut(s)	35
PspN4I	GGNNCC	6 cut(s)	48, 65, 118, 447, 773, 799
PspPI	GGNCC	4 cut(s)	64, 117, 772, 797
PspPPI	RGGWCCY	2 cut(s)	64, 117
PstNI	CAGNNNCTG	1 cut(s)	617
PsuI	RGATCY	1 cut(s)	862
RsaI	GTAC	4 cut(s)	529, 588, 630, 737
RsaNI	GTAC	4 cut(s)	528, 587, 629, 736
RseI	CAYNNNNRTG	1 cut(s)	995
SapI	GCTCTTC	1 cut(s)	830
SaqAI	TTAA	3 cut(s)	81, 522, 602
SatI	GCNGC	2 cut(s)	106, 609
Sau3AI	GATC	3 cut(s)	283, 512, 862
Sau96I	GGNCC	4 cut(s)	64, 117, 772, 797
SchI	GAGTC	1 cut(s)	530
ScrFI	CCNGG	2 cut(s)	37, 728
SduI	GDGCHC	3 cut(s)	448, 903, 1023
SfaNI	GCATC	1 cut(s)	244
SfcI	CTRYAG	1 cut(s)	417
Sfr274I	CTCGAG	1 cut(s)	902
SinI	GGWCC	4 cut(s)	64, 117, 772, 797
SlaI	CTCGAG	1 cut(s)	902
SmiMI	CAYNNNNRTG	1 cut(s)	995
SmlI	CTYRAG	2 cut(s)	902, 938
SmoI	CTYRAG	2 cut(s)	902, 938
SpeI	ACTAGT	1 cut(s)	397
Sse9I	AATT	8 cut(s)	76, 140, 166, 433, 881, 951, 1013, 1030
SsiI	CCGC	7 cut(s)	106, 127, 269, 296, 369, 576, 673
SspMI	CTAG	4 cut(s)	222, 398, 510, 557
StyD4I	CCNGG	2 cut(s)	35, 726
StyI	CCWWGG	1 cut(s)	334
TaaI	ACNGT	2 cut(s)	215, 994
TaiI	ACGT	2 cut(s)	27, 546
TaqI	TCGA	4 cut(s)	210, 235, 816, 903
TasI	AATT	8 cut(s)	76, 140, 166, 433, 881, 951, 1013, 1030
TatI	WGTACW	3 cut(s)	527, 586, 628
TauI	GCSGC	1 cut(s)	108
TfiI	GAWTC	5 cut(s)	32, 237, 614, 621, 680
Tru1I	TTAA	3 cut(s)	81, 522, 602
Tru9I	TTAA	3 cut(s)	81, 522, 602
TseI	GCWGC	1 cut(s)	608
TspDTI	ATGAA	2 cut(s)	30, 536
VpaK11BI	GGWCC	4 cut(s)	64, 117, 772, 797
XapI	RAATTY	2 cut(s)	140, 1013
XbaI	TCTAGA	1 cut(s)	509
XceI	RCATGY	1 cut(s)	786
XcmI	CCANNNNNNNNNTGG	1 cut(s)	57
XhoI	CTCGAG	1 cut(s)	902
XspI	CTAG	4 cut(s)	222, 398, 510, 557

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.