MD04G1213200.v1.1

Belongs to the DHHC palmitoyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0006568
Physical Location & Seq
Forward (+)
29702006 .. 29706031
4026 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1213200.v1.1.491

Sequence Viewer

Length: 1182 bp
ATGGAGGTACAGTGGATTCTGGTGTGCCATGGTTTAGTCACACTACTCGTTCTAGTTTCCTTCCTCTGCGGCCAATGGCCCATCTTTGACGGCACCCCCATACAACGCATCAACCACTTCCTCACCTTCGGCGCCTACGATTACTTCCAACGGTTTGTTGGGTTTGTGTTTGGAGCCAGGGGTACTAATGCCATAGCCTCCGTGGAATATTTATGCTGTGATCGACCCAATCCAATCTTACAGTTCATGTATCTTGCAATAGTAGGAGGAACCTATTACTTAGTTGCAACTTCTTCCTTCAGTTACATTCCTGGCTATTATATTAGTGAAATTCACAGGTATGCAAGCTTGTGGGCTGTTGGTGTTGGTGTTCTGCTCTTTCTATTAGCTAGCTTTTCTGATCCAGGAACAATAAGGGCTGATAATGTTTCTCTGTATCTCTCTGCTTATCCCTATGATAATATTATATATTCCGAGAAAGAATGTTCAACATGCAAAATTCCGAAACCTGCTAGGTCAAAGCATTGCAGCGTATGTGATCGATGTGTTGCCCGTTTTGATCATCATTGTGGATGGATGAATAATTGTATAGGTGAGAGAAACACCCAGTACTTCGTAGCTTTTCTTTTGTGGCACACTTTACTTTGCATATATGGAACAGTGGCCATTGGGTTAATTCTTGCTGGACAAGTTAAAGAATACAAAGTTATTGAAATTCTAACTGTTTATTATGGTGTAGATAATTCTTTTCGCGGTTTAACACCACATGTTGTGCAGTGGTTGTTGGGGGACTACAACACTCAAATACTTCTTATGGTGTTTCTTGCAATAGTTGCACTGCTATTGGCGGGTTTCTTTGGTTACCATGCAAAACTTTGTCTCACAAACACTACAACTAATGAGACGTATAAGTGGCAAGACTACGTAAGTTGGCAGAAGAAGCTAAATGAAGCAAGGGCGAGTTCTGTGGCACTCAAAGCAAGTATCAGTGGGATAAGTAGTGAAAGAAAACCTCCAGAGAGCAAATGGAGAGCCATGTTTCGAAGATCCCCACTCGAAGATGCAGAGGTTGTGGTTAAGAATAACATTTACGATAAAGGATTCTTCCTGAATATTCTCGAGGTCATTGTTCCCCTCTCGAGCAGACTGCGTGCTTCCAGAACCAAATCAAAGTCTGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DHHC

Protein Analysis

394

Amino Acids

44.48

Weight (kDa)

8.76

Isoelectric Point (pI)

33.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DHHC PF01529 158 - 306 2.5e-39 DHHC palmitoyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 517
AccB1I GGYRCC 2 cut(s) 92, 131
AccII CGCG 1 cut(s) 753
AciI CCGC 3 cut(s) 69, 753, 848
AclWI GGATC 2 cut(s) 395, 1041
AcoI YGGCCR 2 cut(s) 70, 663
AcsI RAATTY 3 cut(s) 330, 498, 714
AcuI CTGAAG 1 cut(s) 283
AcyI GRCGYC 1 cut(s) 132
AfaI GTAC 3 cut(s) 9, 184, 611
AflIII ACRYGT 1 cut(s) 766
AgsI TTSAA 2 cut(s) 489, 713
AjnI CCWGG 3 cut(s) 176, 310, 403
AluBI AGCT 5 cut(s) 348, 389, 393, 620, 943
AluI AGCT 5 cut(s) 348, 389, 393, 620, 943
Alw26I GTCTC 2 cut(s) 884, 896
AlwI GGATC 2 cut(s) 395, 1041
Ama87I CYCGRG 2 cut(s) 1118, 1138
AoxI GGCC 3 cut(s) 70, 77, 663
ApeKI GCWGC 1 cut(s) 528
ApoI RAATTY 3 cut(s) 330, 498, 714
AspLEI GCGC 1 cut(s) 134
AspS9I GGNCC 1 cut(s) 78
AsuHPI GGTGA 2 cut(s) 115, 605
AsuII TTCGAA 1 cut(s) 1042
AsuNHI GCTAGC 1 cut(s) 389
AvaI CYCGRG 2 cut(s) 1118, 1138
BalI TGGCCA 1 cut(s) 665
BanI GGYRCC 2 cut(s) 92, 131
BbvI GCAGC 1 cut(s) 540
BccI CCATC 2 cut(s) 89, 567
BceAI ACGGC 1 cut(s) 106
BcgI CGANNNNNNTGC 2 cut(s) 1129, 1163
BciT130I CCWGG 3 cut(s) 178, 312, 405
BclI TGATCA 1 cut(s) 559
BcoDI GTCTC 2 cut(s) 884, 896
BfaI CTAG 3 cut(s) 53, 390, 513
BfoI RGCGCY 1 cut(s) 135
BfuAI ACCTGC 1 cut(s) 517
BisI GCNGC 2 cut(s) 70, 529
BlsI GCNGC 2 cut(s) 71, 530
BmcAI AGTACT 1 cut(s) 611
Bme1390I CCNGG 3 cut(s) 178, 312, 405
BmeT110I CYCGRG 2 cut(s) 1118, 1138
BmgT120I GGNCC 1 cut(s) 78
BmiI GGNNCC 4 cut(s) 94, 133, 175, 271
BmrFI CCNGG 3 cut(s) 178, 312, 405
BmrI ACTGGG 1 cut(s) 601
BmsI GCATC 2 cut(s) 117, 1051
BmtI GCTAGC 1 cut(s) 393
BmuI ACTGGG 1 cut(s) 601
BpmI CTGGAG 1 cut(s) 999
Bpu14I TTCGAA 1 cut(s) 1042
Bsa29I ATCGAT 1 cut(s) 541
BsaAI YACGTR 1 cut(s) 925
BsaHI GRCGYC 1 cut(s) 132
BsaJI CCNNGG 3 cut(s) 28, 177, 201
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bse1I ACTGG 1 cut(s) 607
Bse3DI GCAATG 1 cut(s) 523
BseBI CCWGG 3 cut(s) 178, 312, 405
BseCI ATCGAT 1 cut(s) 541
BseDI CCNNGG 3 cut(s) 28, 177, 201
BseGI GGATG 2 cut(s) 578, 582
BseMI GCAATG 1 cut(s) 523
BseNI ACTGG 1 cut(s) 607
BseXI GCAGC 1 cut(s) 540
BsgI GTGCAG 1 cut(s) 794
Bsh1236I CGCG 1 cut(s) 753
BshFI GGCC 3 cut(s) 72, 79, 665
BshNI GGYRCC 2 cut(s) 92, 131
BshVI ATCGAT 1 cut(s) 541
BsiHKCI CYCGRG 2 cut(s) 1118, 1138
BslFI GGGAC 1 cut(s) 803
BsmAI GTCTC 2 cut(s) 884, 896
BsmBI CGTCTC 1 cut(s) 896
BsmFI GGGAC 1 cut(s) 803
BsnI GGCC 3 cut(s) 72, 79, 665
BsoBI CYCGRG 2 cut(s) 1118, 1138
Bsp119I TTCGAA 1 cut(s) 1042
Bsp143I GATC 5 cut(s) 220, 400, 538, 559, 1046
Bsp19I CCATGG 1 cut(s) 28
BspACI CCGC 3 cut(s) 69, 753, 848
BspANI GGCC 3 cut(s) 72, 79, 665
BspDI ATCGAT 1 cut(s) 541
BspFNI CGCG 1 cut(s) 753
BspLI GGNNCC 4 cut(s) 94, 133, 175, 271
BspMI ACCTGC 1 cut(s) 517
BspOI GCTAGC 1 cut(s) 393
BspPI GGATC 2 cut(s) 395, 1041
BspT104I TTCGAA 1 cut(s) 1042
BspT107I GGYRCC 2 cut(s) 92, 131
BsrDI GCAATG 1 cut(s) 523
BsrI ACTGG 1 cut(s) 607
BssECI CCNNGG 3 cut(s) 28, 177, 201
BssMI GATC 5 cut(s) 220, 400, 538, 559, 1046
BssNI GRCGYC 1 cut(s) 132
BssT1I CCWWGG 1 cut(s) 28
Bst2UI CCWGG 3 cut(s) 178, 312, 405
Bst4CI ACNGT 5 cut(s) 12, 153, 243, 661, 724
BstACI GRCGYC 1 cut(s) 132
BstAPI GCANNNNNTGC 1 cut(s) 833
BstBAI YACGTR 1 cut(s) 925
BstBI TTCGAA 1 cut(s) 1042
BstC8I GCNNGC 3 cut(s) 346, 391, 1152
BstDEI CTNAG 1 cut(s) 280
BstDSI CCRYGG 2 cut(s) 28, 201
BstEII GGTNACC 1 cut(s) 860
BstF5I GGATG 2 cut(s) 578, 582
BstFNI CGCG 1 cut(s) 753
BstH2I RGCGCY 1 cut(s) 135
BstHHI GCGC 1 cut(s) 134
BstKTI GATC 5 cut(s) 223, 403, 541, 562, 1049
BstMAI GTCTC 2 cut(s) 884, 896
BstMBI GATC 5 cut(s) 220, 400, 538, 559, 1046
BstMWI GCNNNNNNNGC 3 cut(s) 833, 940, 977
BstNI CCWGG 3 cut(s) 178, 312, 405
BstNSI RCATGY 2 cut(s) 495, 770
BstPI GGTNACC 1 cut(s) 860
BstSCI CCNGG 3 cut(s) 176, 310, 403
BstSNI TACGTA 1 cut(s) 925
BstUI CGCG 1 cut(s) 753
BstV1I GCAGC 1 cut(s) 540
BstX2I RGATCY 1 cut(s) 1046
BstYI RGATCY 1 cut(s) 1046
Bsu15I ATCGAT 1 cut(s) 541
BsuRI GGCC 3 cut(s) 72, 79, 665
BsuTUI ATCGAT 1 cut(s) 541
BtgI CCRYGG 2 cut(s) 28, 201
BtsCI GGATG 2 cut(s) 578, 582
BtsI GCAGTG 2 cut(s) 782, 836
BtsIMutI CAGTG 5 cut(s) 17, 666, 782, 836, 994
BveI ACCTGC 1 cut(s) 517
Cac8I GCNNGC 3 cut(s) 346, 391, 1152
CfoI GCGC 1 cut(s) 134
Cfr13I GGNCC 1 cut(s) 78
ClaI ATCGAT 1 cut(s) 541
Csp6I GTAC 3 cut(s) 8, 183, 610
CviAII CATG 6 cut(s) 29, 247, 492, 767, 866, 1036
CviQI GTAC 3 cut(s) 8, 183, 610
DdeI CTNAG 1 cut(s) 280
DinI GGCGCC 1 cut(s) 133
DpnI GATC 5 cut(s) 222, 402, 540, 561, 1048
DpnII GATC 5 cut(s) 220, 400, 538, 559, 1046
EaeI YGGCCR 2 cut(s) 70, 663
Eco105I TACGTA 1 cut(s) 925
Eco130I CCWWGG 1 cut(s) 28
Eco57I CTGAAG 1 cut(s) 283
Eco88I CYCGRG 2 cut(s) 1118, 1138
Eco91I GGTNACC 1 cut(s) 860
EcoO65I GGTNACC 1 cut(s) 860
EcoRII CCWGG 3 cut(s) 176, 310, 403
EcoT14I CCWWGG 1 cut(s) 28
EgeI GGCGCC 1 cut(s) 133
EheI GGCGCC 1 cut(s) 133
ErhI CCWWGG 1 cut(s) 28
Esp3I CGTCTC 1 cut(s) 896
FaeI CATG 6 cut(s) 32, 250, 495, 770, 869, 1039
FaqI GGGAC 1 cut(s) 803
FatI CATG 6 cut(s) 28, 246, 491, 766, 865, 1035
FauI CCCGC 1 cut(s) 841
FbaI TGATCA 1 cut(s) 559
Fnu4HI GCNGC 2 cut(s) 70, 529
FokI GGATG 2 cut(s) 585, 589
Fsp4HI GCNGC 2 cut(s) 70, 529
FspBI CTAG 3 cut(s) 53, 390, 513
GlaI GCGC 1 cut(s) 133
GluI GCNGC 2 cut(s) 70, 529
GsuI CTGGAG 1 cut(s) 999
HaeII RGCGCY 1 cut(s) 135
HaeIII GGCC 3 cut(s) 72, 79, 665
HhaI GCGC 1 cut(s) 134
Hin1I GRCGYC 1 cut(s) 132
Hin1II CATG 6 cut(s) 32, 250, 495, 770, 869, 1039
Hin6I GCGC 1 cut(s) 132
HinP1I GCGC 1 cut(s) 132
HindIII AAGCTT 1 cut(s) 346
HinfI GANTC 2 cut(s) 16, 1101
HphI GGTGA 2 cut(s) 115, 605
Hpy188I TCNGA 3 cut(s) 400, 475, 504
Hpy188III TCNNGA 5 cut(s) 1016, 1108, 1118, 1138, 1158
HpyAV CCTTC 3 cut(s) 70, 136, 307
HpyCH4III ACNGT 5 cut(s) 12, 153, 243, 661, 724
HpyCH4IV ACGT 2 cut(s) 905, 924
HpyF10VI GCNNNNNNNGC 3 cut(s) 833, 940, 977
HpyF3I CTNAG 1 cut(s) 280
HpySE526I ACGT 2 cut(s) 905, 924
Hsp92I GRCGYC 1 cut(s) 132
Hsp92II CATG 6 cut(s) 32, 250, 495, 770, 869, 1039
HspAI GCGC 1 cut(s) 132
KasI GGCGCC 1 cut(s) 131
Ksp22I TGATCA 1 cut(s) 559
Kzo9I GATC 5 cut(s) 220, 400, 538, 559, 1046
LmnI GCTCC 1 cut(s) 173
Lsp1109I GCAGC 1 cut(s) 540
LweI GCATC 2 cut(s) 117, 1051
MaeI CTAG 3 cut(s) 53, 390, 513
MaeII ACGT 2 cut(s) 905, 924
MaeIII GTNAC 3 cut(s) 37, 302, 860
MalI GATC 5 cut(s) 222, 402, 540, 561, 1048
MboI GATC 5 cut(s) 220, 400, 538, 559, 1046
MboII GAAGA 5 cut(s) 285, 949, 1056, 1070, 1096
MflI RGATCY 1 cut(s) 1046
MlsI TGGCCA 1 cut(s) 665
MluCI AATT 6 cut(s) 330, 498, 583, 675, 714, 742
MluNI TGGCCA 1 cut(s) 665
Mly113I GGCGCC 1 cut(s) 132
MmeI TCCRAC 1 cut(s) 172
MnlI CCTC 8 cut(s) 74, 131, 208, 260, 1023, 1060, 1114, 1145
Mox20I TGGCCA 1 cut(s) 665
MscI TGGCCA 1 cut(s) 665
MseI TTAA 4 cut(s) 674, 693, 758, 1077
MslI CAYNNNNRTG 2 cut(s) 339, 567
Msp20I TGGCCA 1 cut(s) 665
MspR9I CCNGG 3 cut(s) 178, 312, 405
MvaI CCWGG 3 cut(s) 178, 312, 405
MvnI CGCG 1 cut(s) 753
MwoI GCNNNNNNNGC 3 cut(s) 833, 940, 977
NarI GGCGCC 1 cut(s) 132
NcoI CCATGG 1 cut(s) 28
NdeII GATC 5 cut(s) 220, 400, 538, 559, 1046
NheI GCTAGC 1 cut(s) 389
NlaIII CATG 6 cut(s) 32, 250, 495, 770, 869, 1039
NlaIV GGNNCC 4 cut(s) 94, 133, 175, 271
NmuCI GTSAC 1 cut(s) 37
NspI RCATGY 2 cut(s) 495, 770
NspV TTCGAA 1 cut(s) 1042
PaeR7I CTCGAG 2 cut(s) 1118, 1138
PciI ACATGT 1 cut(s) 766
PcsI WCGNNNNNNNCGW 1 cut(s) 135
PfeI GAWTC 2 cut(s) 16, 1101
PfoI TCCNGGA 1 cut(s) 403
PkrI GCNGC 2 cut(s) 71, 530
PluTI GGCGCC 1 cut(s) 135
Ppu21I YACGTR 1 cut(s) 925
PscI ACATGT 1 cut(s) 766
Psp6I CCWGG 3 cut(s) 176, 310, 403
PspEI GGTNACC 1 cut(s) 860
PspGI CCWGG 3 cut(s) 176, 310, 403
PspN4I GGNNCC 4 cut(s) 94, 133, 175, 271
PspPI GGNCC 1 cut(s) 78
PsuI RGATCY 1 cut(s) 1046
RsaI GTAC 3 cut(s) 9, 184, 611
RsaNI GTAC 3 cut(s) 8, 183, 610
RseI CAYNNNNRTG 2 cut(s) 339, 567
SaqAI TTAA 4 cut(s) 674, 693, 758, 1077
SatI GCNGC 2 cut(s) 70, 529
Sau3AI GATC 5 cut(s) 220, 400, 538, 559, 1046
Sau96I GGNCC 1 cut(s) 78
ScaI AGTACT 1 cut(s) 611
ScrFI CCNGG 3 cut(s) 178, 312, 405
SfaNI GCATC 2 cut(s) 117, 1051
SfoI GGCGCC 1 cut(s) 133
Sfr274I CTCGAG 2 cut(s) 1118, 1138
SfuI TTCGAA 1 cut(s) 1042
SlaI CTCGAG 2 cut(s) 1118, 1138
SmiMI CAYNNNNRTG 2 cut(s) 339, 567
SmlI CTYRAG 2 cut(s) 1118, 1138
SmoI CTYRAG 2 cut(s) 1118, 1138
SnaBI TACGTA 1 cut(s) 925
Sse9I AATT 6 cut(s) 330, 498, 583, 675, 714, 742
SsiI CCGC 3 cut(s) 69, 753, 848
SspDI GGCGCC 1 cut(s) 131
SspI AATATT 3 cut(s) 209, 463, 1114
SspMI CTAG 3 cut(s) 53, 390, 513
StyD4I CCNGG 3 cut(s) 176, 310, 403
StyI CCWWGG 1 cut(s) 28
TaaI ACNGT 5 cut(s) 12, 153, 243, 661, 724
TaiI ACGT 2 cut(s) 908, 927
TaqI TCGA 6 cut(s) 223, 541, 1042, 1056, 1119, 1139
TasI AATT 6 cut(s) 330, 498, 583, 675, 714, 742
TatI WGTACW 1 cut(s) 609
TauI GCSGC 1 cut(s) 72
TfiI GAWTC 2 cut(s) 16, 1101
Tru1I TTAA 4 cut(s) 674, 693, 758, 1077
Tru9I TTAA 4 cut(s) 674, 693, 758, 1077
TscAI CASTG 5 cut(s) 17, 666, 782, 843, 994
TseFI GTSAC 1 cut(s) 37
TseI GCWGC 1 cut(s) 528
Tsp45I GTSAC 1 cut(s) 37
TspDTI ATGAA 3 cut(s) 235, 593, 963
TspGWI ACGGA 1 cut(s) 190
TspRI CASTG 5 cut(s) 17, 666, 782, 843, 994
XapI RAATTY 3 cut(s) 330, 498, 714
XceI RCATGY 2 cut(s) 495, 770
XcmI CCANNNNNNNNNTGG 3 cut(s) 155, 199, 1023
XhoI CTCGAG 2 cut(s) 1118, 1138
XspI CTAG 3 cut(s) 53, 390, 513
ZrmI AGTACT 1 cut(s) 611
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.