MD12G1227400.v1.1

Belongs to the DHHC palmitoyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0006568
Physical Location & Seq
Forward (+)
30299937 .. 30304100
4164 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1227400.v1.1.491

Sequence Viewer

Length: 1182 bp
ATGGAGGTACAGTGGATTCTGGTGTGCCATGGTTTAGTCACACTGCTCGTTCTAGTCTCCTTCCTCTGCGGCCAATGGCCCATCTTCGACGGCACCCCCATCCAACGCATCAACCACTTCCTCACCTTCGGCGCCTACGATTACTTTCAACGGTTTGTTGGGTTTGTGTTTGGAGCCAGGGGGACAAATGCCATAGCTTCCGCGGAGTATTTCTGCTGCGATCGACCCAATCCAATCTTACAGATCATTTATTTTGCAATAGTAGGAGGAACCTATTACTTGGTTGCAACTTCTTCCTTCCGTTACATTCCTGGCTATTATATAAGTGAAATTCACAGGTATGCGAGCTTGTTGGCTGTTGGCGTTGGTGTCCTACTCTTTCTATTGGCTAGCTTTTCTGATCCAGGAACGGTGAAAGCTGATAATGTTTCTCTGTATCTCTTTGCTTATCCTTACGATAATATTATATATTCTGAGAAAGACTGTTCAACATGCAAAATTCCAAAACCTGCTAGGTCAAAGCATTGCAGCATATGTGATCGATGTGTTGCCCGTTTTGATCATCATTGTGGATGGATGAATAATTGTATAGGTGAGAGAAACACCCGGTACTTCATAGCTTTTCTTTTGTGGCACACTTTACTTTGCATATATGGAACAGTGGCGATTGGGTTAATTCTTGCTGGACAAGTTAAAGAATTCAAAGTTATTGATATTCTAACCGTTTATTATGGTGTAGATAATTCTTTTCGCCGTTTAACGCCACATGTTGTGCAGTGGTTGTTGGGCGCCTACAACATTCAAATACTTCTAATGGTGTTTCTTGCAATTGTTTCACTACTATTGGCGGGTTTCTTTGGTTACCATGCAAAACTTTGTCTTTCAAACACTACAACTAATGAGACTTTCAAGTGGCAAGACTACTTAAGCTGGCAGAGGAAGCTGAATGAAGCAAGGGCGAGTTCTGCAGCACTCAAAGCAAGTATCAATGGGACAGATAGTGAAAGAAAACCTCCAGAGAGCAAATGGAGAGCCATTTTCCGAAGATCACCACTCAAAGAGGTGGATGTTGTGGTTAAAAATAACATGTACGATAAAGGATTCTTCCAGAATATTCTTGAGGTCATTGTTCCCCTCTCAAGAAGACCGCTGGCTTCGCAAACCAAATCAAAGTCTGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DHHC

Protein Analysis

394

Amino Acids

44.63

Weight (kDa)

9.06

Isoelectric Point (pI)

31.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DHHC PF01529 158 - 306 8.4e-39 DHHC palmitoyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 517
AccB1I GGYRCC 3 cut(s) 92, 131, 788
AccII CGCG 1 cut(s) 203
AciI CCGC 5 cut(s) 69, 201, 203, 848, 1148
AclWI GGATC 1 cut(s) 395
AcoI YGGCCR 1 cut(s) 70
AcsI RAATTY 3 cut(s) 330, 498, 698
AcyI GRCGYC 2 cut(s) 132, 789
AfaI GTAC 3 cut(s) 9, 611, 1091
AflII CTTAAG 1 cut(s) 925
AflIII ACRYGT 2 cut(s) 766, 1086
AgsI TTSAA 6 cut(s) 149, 489, 703, 803, 885, 910
AjnI CCWGG 3 cut(s) 176, 310, 403
AluBI AGCT 7 cut(s) 197, 348, 393, 419, 620, 930, 943
AluI AGCT 7 cut(s) 197, 348, 393, 419, 620, 930, 943
Alw26I GTCTC 2 cut(s) 61, 896
AlwI GGATC 1 cut(s) 395
AoxI GGCC 2 cut(s) 70, 77
ApeKI GCWGC 3 cut(s) 216, 528, 968
ApoI RAATTY 3 cut(s) 330, 498, 698
AspLEI GCGC 2 cut(s) 134, 791
AspS9I GGNCC 1 cut(s) 78
AsuC2I CCSGG 1 cut(s) 607
AsuHPI GGTGA 4 cut(s) 115, 424, 605, 1041
AsuNHI GCTAGC 1 cut(s) 389
BanI GGYRCC 3 cut(s) 92, 131, 788
BbsI GAAGAC 1 cut(s) 1150
BbvI GCAGC 3 cut(s) 203, 540, 980
BccI CCATC 3 cut(s) 89, 107, 567
BceAI ACGGC 2 cut(s) 106, 738
BciT130I CCWGG 3 cut(s) 178, 312, 405
BclI TGATCA 1 cut(s) 559
BcnI CCSGG 1 cut(s) 607
BcoDI GTCTC 2 cut(s) 61, 896
BfaI CTAG 3 cut(s) 53, 390, 513
BfmI CTRYAG 1 cut(s) 966
BfoI RGCGCY 2 cut(s) 135, 792
BfrI CTTAAG 1 cut(s) 925
BfuAI ACCTGC 1 cut(s) 517
BisI GCNGC 4 cut(s) 70, 217, 529, 969
BlsI GCNGC 4 cut(s) 71, 218, 530, 970
Bme1390I CCNGG 4 cut(s) 178, 312, 405, 607
BmgT120I GGNCC 1 cut(s) 78
BmiI GGNNCC 5 cut(s) 94, 133, 175, 271, 790
BmrFI CCNGG 4 cut(s) 178, 312, 405, 607
BmsI GCATC 1 cut(s) 117
BmtI GCTAGC 1 cut(s) 393
BpiI GAAGAC 1 cut(s) 1150
BpmI CTGGAG 1 cut(s) 999
BpuEI CTTGAG 2 cut(s) 1123, 1139
BpuMI CCSGG 1 cut(s) 607
Bsa29I ATCGAT 1 cut(s) 541
BsaHI GRCGYC 2 cut(s) 132, 789
BsaJI CCNNGG 3 cut(s) 28, 177, 201
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bse3DI GCAATG 1 cut(s) 523
BseBI CCWGG 3 cut(s) 178, 312, 405
BseCI ATCGAT 1 cut(s) 541
BseDI CCNNGG 3 cut(s) 28, 177, 201
BseGI GGATG 4 cut(s) 99, 578, 582, 1072
BseMI GCAATG 1 cut(s) 523
BseMII CTCAG 1 cut(s) 465
BseXI GCAGC 3 cut(s) 203, 540, 980
BsgI GTGCAG 1 cut(s) 794
Bsh1236I CGCG 1 cut(s) 203
Bsh1285I CGRYCG 1 cut(s) 223
BshFI GGCC 2 cut(s) 72, 79
BshNI GGYRCC 3 cut(s) 92, 131, 788
BshVI ATCGAT 1 cut(s) 541
BsiEI CGRYCG 1 cut(s) 223
BsiSI CCGG 1 cut(s) 607
BslFI GGGAC 2 cut(s) 196, 1006
BsmAI GTCTC 2 cut(s) 61, 896
BsmFI GGGAC 2 cut(s) 196, 1006
BsnI GGCC 2 cut(s) 72, 79
Bsp143I GATC 6 cut(s) 220, 243, 400, 538, 559, 1046
Bsp19I CCATGG 1 cut(s) 28
BspACI CCGC 5 cut(s) 69, 201, 203, 848, 1148
BspANI GGCC 2 cut(s) 72, 79
BspCNI CTCAG 1 cut(s) 466
BspDI ATCGAT 1 cut(s) 541
BspFNI CGCG 1 cut(s) 203
BspLI GGNNCC 5 cut(s) 94, 133, 175, 271, 790
BspMAI CTGCAG 1 cut(s) 970
BspMI ACCTGC 1 cut(s) 517
BspOI GCTAGC 1 cut(s) 393
BspPI GGATC 1 cut(s) 395
BspT107I GGYRCC 3 cut(s) 92, 131, 788
BspTI CTTAAG 1 cut(s) 925
BsrDI GCAATG 1 cut(s) 523
BssECI CCNNGG 3 cut(s) 28, 177, 201
BssMI GATC 6 cut(s) 220, 243, 400, 538, 559, 1046
BssNI GRCGYC 2 cut(s) 132, 789
BssT1I CCWWGG 1 cut(s) 28
Bst2UI CCWGG 3 cut(s) 178, 312, 405
Bst4CI ACNGT 6 cut(s) 12, 153, 412, 485, 661, 724
BstACI GRCGYC 2 cut(s) 132, 789
BstAFI CTTAAG 1 cut(s) 925
BstC8I GCNNGC 4 cut(s) 346, 391, 932, 1152
BstDEI CTNAG 1 cut(s) 474
BstDSI CCRYGG 2 cut(s) 28, 201
BstEII GGTNACC 1 cut(s) 860
BstF5I GGATG 4 cut(s) 99, 578, 582, 1072
BstFNI CGCG 1 cut(s) 203
BstH2I RGCGCY 2 cut(s) 135, 792
BstHHI GCGC 2 cut(s) 134, 791
BstKTI GATC 6 cut(s) 223, 246, 403, 541, 562, 1049
BstMAI GTCTC 2 cut(s) 61, 896
BstMBI GATC 6 cut(s) 220, 243, 400, 538, 559, 1046
BstMCI CGRYCG 1 cut(s) 223
BstMWI GCNNNNNNNGC 4 cut(s) 940, 965, 977, 1156
BstNI CCWGG 3 cut(s) 178, 312, 405
BstNSI RCATGY 3 cut(s) 495, 770, 1090
BstPI GGTNACC 1 cut(s) 860
BstSCI CCNGG 4 cut(s) 176, 310, 403, 605
BstSFI CTRYAG 1 cut(s) 966
BstUI CGCG 1 cut(s) 203
BstV1I GCAGC 3 cut(s) 203, 540, 980
BstV2I GAAGAC 1 cut(s) 1150
Bsu15I ATCGAT 1 cut(s) 541
BsuRI GGCC 2 cut(s) 72, 79
BsuTUI ATCGAT 1 cut(s) 541
BtgI CCRYGG 2 cut(s) 28, 201
BtsCI GGATG 4 cut(s) 99, 578, 582, 1072
BtsI GCAGTG 2 cut(s) 41, 782
BtsIMutI CAGTG 4 cut(s) 17, 41, 666, 782
BveI ACCTGC 1 cut(s) 517
Cac8I GCNNGC 4 cut(s) 346, 391, 932, 1152
CfoI GCGC 2 cut(s) 134, 791
Cfr13I GGNCC 1 cut(s) 78
Cfr42I CCGCGG 1 cut(s) 204
ClaI ATCGAT 1 cut(s) 541
Csp6I GTAC 3 cut(s) 8, 610, 1090
CviAII CATG 5 cut(s) 29, 492, 767, 866, 1087
CviQI GTAC 3 cut(s) 8, 610, 1090
DdeI CTNAG 1 cut(s) 474
DinI GGCGCC 2 cut(s) 133, 790
DpnI GATC 6 cut(s) 222, 245, 402, 540, 561, 1048
DpnII GATC 6 cut(s) 220, 243, 400, 538, 559, 1046
EaeI YGGCCR 1 cut(s) 70
Eco130I CCWWGG 1 cut(s) 28
Eco91I GGTNACC 1 cut(s) 860
EcoO65I GGTNACC 1 cut(s) 860
EcoRI GAATTC 1 cut(s) 698
EcoRII CCWGG 3 cut(s) 176, 310, 403
EcoT14I CCWWGG 1 cut(s) 28
EgeI GGCGCC 2 cut(s) 133, 790
EheI GGCGCC 2 cut(s) 133, 790
ErhI CCWWGG 1 cut(s) 28
FaeI CATG 5 cut(s) 32, 495, 770, 869, 1090
FaqI GGGAC 2 cut(s) 196, 1006
FatI CATG 5 cut(s) 28, 491, 766, 865, 1086
FauI CCCGC 1 cut(s) 841
FauNDI CATATG 1 cut(s) 533
FbaI TGATCA 1 cut(s) 559
Fnu4HI GCNGC 4 cut(s) 70, 217, 529, 969
FokI GGATG 4 cut(s) 86, 585, 589, 1079
Fsp4HI GCNGC 4 cut(s) 70, 217, 529, 969
FspBI CTAG 3 cut(s) 53, 390, 513
GlaI GCGC 2 cut(s) 133, 790
GluI GCNGC 4 cut(s) 70, 217, 529, 969
GsuI CTGGAG 1 cut(s) 999
HaeII RGCGCY 2 cut(s) 135, 792
HaeIII GGCC 2 cut(s) 72, 79
HapII CCGG 1 cut(s) 607
HhaI GCGC 2 cut(s) 134, 791
Hin1I GRCGYC 2 cut(s) 132, 789
Hin1II CATG 5 cut(s) 32, 495, 770, 869, 1090
Hin6I GCGC 2 cut(s) 132, 789
HinP1I GCGC 2 cut(s) 132, 789
HinfI GANTC 2 cut(s) 16, 1101
HpaII CCGG 1 cut(s) 607
HphI GGTGA 4 cut(s) 115, 424, 605, 1041
Hpy188I TCNGA 3 cut(s) 400, 475, 1043
Hpy188III TCNNGA 4 cut(s) 1016, 1108, 1118, 1140
Hpy99I CGWCG 1 cut(s) 92
HpyAV CCTTC 3 cut(s) 70, 136, 307
HpyCH4III ACNGT 6 cut(s) 12, 153, 412, 485, 661, 724
HpyCH4V TGCA 9 cut(s) 257, 287, 495, 528, 648, 775, 827, 869, 968
HpyF10VI GCNNNNNNNGC 4 cut(s) 940, 965, 977, 1156
HpyF3I CTNAG 1 cut(s) 474
Hsp92I GRCGYC 2 cut(s) 132, 789
Hsp92II CATG 5 cut(s) 32, 495, 770, 869, 1090
HspAI GCGC 2 cut(s) 132, 789
KasI GGCGCC 2 cut(s) 131, 788
Ksp22I TGATCA 1 cut(s) 559
KspI CCGCGG 1 cut(s) 204
Kzo9I GATC 6 cut(s) 220, 243, 400, 538, 559, 1046
LmnI GCTCC 1 cut(s) 173
Lsp1109I GCAGC 3 cut(s) 203, 540, 980
LweI GCATC 1 cut(s) 117
MaeI CTAG 3 cut(s) 53, 390, 513
MaeIII GTNAC 3 cut(s) 37, 302, 860
MalI GATC 6 cut(s) 222, 245, 402, 540, 561, 1048
MboI GATC 6 cut(s) 220, 243, 400, 538, 559, 1046
MboII GAAGA 5 cut(s) 76, 285, 1056, 1096, 1155
MfeI CAATTG 1 cut(s) 828
MluCI AATT 7 cut(s) 330, 498, 583, 675, 698, 742, 828
Mly113I GGCGCC 2 cut(s) 132, 789
MmeI TCCRAC 1 cut(s) 127
MnlI CCTC 8 cut(s) 74, 131, 260, 930, 1023, 1054, 1114, 1145
MseI TTAA 5 cut(s) 674, 693, 758, 926, 1077
MslI CAYNNNNRTG 2 cut(s) 339, 567
MspA1I CMGCKG 2 cut(s) 203, 1150
MspCI CTTAAG 1 cut(s) 925
MspI CCGG 1 cut(s) 607
MspR9I CCNGG 4 cut(s) 178, 312, 405, 607
MunI CAATTG 1 cut(s) 828
MvaI CCWGG 3 cut(s) 178, 312, 405
MvnI CGCG 1 cut(s) 203
MwoI GCNNNNNNNGC 4 cut(s) 940, 965, 977, 1156
NarI GGCGCC 2 cut(s) 132, 789
NciI CCSGG 1 cut(s) 607
NcoI CCATGG 1 cut(s) 28
NdeI CATATG 1 cut(s) 533
NdeII GATC 6 cut(s) 220, 243, 400, 538, 559, 1046
NheI GCTAGC 1 cut(s) 389
NlaIII CATG 5 cut(s) 32, 495, 770, 869, 1090
NlaIV GGNNCC 5 cut(s) 94, 133, 175, 271, 790
NmuCI GTSAC 1 cut(s) 37
NspI RCATGY 3 cut(s) 495, 770, 1090
PciI ACATGT 2 cut(s) 766, 1086
PcsI WCGNNNNNNNCGW 1 cut(s) 135
PfeI GAWTC 2 cut(s) 16, 1101
PfoI TCCNGGA 1 cut(s) 403
PkrI GCNGC 4 cut(s) 71, 218, 530, 970
Ple19I CGATCG 1 cut(s) 223
PluTI GGCGCC 2 cut(s) 135, 792
PscI ACATGT 2 cut(s) 766, 1086
Psp6I CCWGG 3 cut(s) 176, 310, 403
PspEI GGTNACC 1 cut(s) 860
PspGI CCWGG 3 cut(s) 176, 310, 403
PspN4I GGNNCC 5 cut(s) 94, 133, 175, 271, 790
PspPI GGNCC 1 cut(s) 78
PstI CTGCAG 1 cut(s) 970
PvuI CGATCG 1 cut(s) 223
RsaI GTAC 3 cut(s) 9, 611, 1091
RsaNI GTAC 3 cut(s) 8, 610, 1090
RseI CAYNNNNRTG 2 cut(s) 339, 567
SacII CCGCGG 1 cut(s) 204
SaqAI TTAA 5 cut(s) 674, 693, 758, 926, 1077
SatI GCNGC 4 cut(s) 70, 217, 529, 969
Sau3AI GATC 6 cut(s) 220, 243, 400, 538, 559, 1046
Sau96I GGNCC 1 cut(s) 78
ScrFI CCNGG 4 cut(s) 178, 312, 405, 607
SfaNI GCATC 1 cut(s) 117
SfcI CTRYAG 1 cut(s) 966
SfoI GGCGCC 2 cut(s) 133, 790
Sfr303I CCGCGG 1 cut(s) 204
SgrBI CCGCGG 1 cut(s) 204
SmiMI CAYNNNNRTG 2 cut(s) 339, 567
SmlI CTYRAG 3 cut(s) 925, 1118, 1138
SmoI CTYRAG 3 cut(s) 925, 1118, 1138
Sse9I AATT 7 cut(s) 330, 498, 583, 675, 698, 742, 828
SsiI CCGC 5 cut(s) 69, 201, 203, 848, 1148
SspDI GGCGCC 2 cut(s) 131, 788
SspI AATATT 2 cut(s) 463, 1114
SspMI CTAG 3 cut(s) 53, 390, 513
StyD4I CCNGG 4 cut(s) 176, 310, 403, 605
StyI CCWWGG 1 cut(s) 28
TaaI ACNGT 6 cut(s) 12, 153, 412, 485, 661, 724
TaqI TCGA 3 cut(s) 87, 223, 541
TasI AATT 7 cut(s) 330, 498, 583, 675, 698, 742, 828
TauI GCSGC 1 cut(s) 72
TfiI GAWTC 2 cut(s) 16, 1101
Tru1I TTAA 5 cut(s) 674, 693, 758, 926, 1077
Tru9I TTAA 5 cut(s) 674, 693, 758, 926, 1077
TscAI CASTG 4 cut(s) 17, 48, 666, 782
TseFI GTSAC 1 cut(s) 37
TseI GCWGC 3 cut(s) 216, 528, 968
Tsp45I GTSAC 1 cut(s) 37
TspDTI ATGAA 3 cut(s) 593, 604, 963
TspGWI ACGGA 1 cut(s) 290
TspRI CASTG 4 cut(s) 17, 48, 666, 782
Vha464I CTTAAG 1 cut(s) 925
XapI RAATTY 3 cut(s) 330, 498, 698
XceI RCATGY 3 cut(s) 495, 770, 1090
XcmI CCANNNNNNNNNTGG 1 cut(s) 1023
XspI CTAG 3 cut(s) 53, 390, 513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.