Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0005571

Physical Location & Seq

Genomic Coordinates Forward (+)

30716459 .. 30727232

Gene Structure 10774 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1227800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4590 bp

ATGGGAACGCCAGTGAATATCATTGTAGGTTCTCATGTCTGGGTTGAAGACCCAGAATTGGCTTGGATCGACGGACAGGTGTCAAAGATTACTGGACAGGAAGCTGAGATCCAGGGTAGCAATGGGAAGAAGATGGTTGTCAAATTGGCAAAAATATATCCAAAAGATATGGAAGCTCCTGCTGCCGGAGTTGACGACATGACAAAGTTATCTTATCTGCATGAGCCTGGAGTTCTGCAGAATTTAAAAACCAGATACGAACTTAATGAAATCTATACATATACTGGAAATATCCTTATTGCCATTAACCCATTCCAAAGACTTCCCCATCTTTATGATGGTCACATGATGCAACAATACAAAGGAGCACCACTTGGAGAATTGAGCCCTCATGTTTTTGCAGTTGCAGATGTTGCATATAGGGCAATGATCAATGAGGGAAAAAGCAATTCAATTCTGGTCAGTGGTGAAAGTGGAGCAGGTAAAACTGAGACCACAAAAATGCTTATGCGGTACCTTGCATTTCTTGGTGGCCGGGCTGCAACTGAAGGACGGACAGTTGAACAGCAAGTTCTTGAATCCAATCCAGTTCTTGAAGCATTTGGCAATGCCAAAACTGTTAGAAATAACAACTCTAGTCGTTTTGGTAAATTTGTTGAAATCCAATTTGATAAGCAAGGAAGAATCTCAGGAGCAGCCATTAGAACTTACCTTCTGGAACGGTCTCGTGTTTGCCAAATCTCTGATCCTGAACGGAACTACCATTGCTTCTACCTTCTTTGTGCTGCACCACAGGAGGAAATTGATAAATACAAGTTGGCAAATCCTAAATCATTTCGCTATCTTAACCAGTCGCGCTGCTACGAACTGGTTGGTGTTAGTGATGCCCATGAATATCTTGCCACAAGGAGAGCTATGGATATTGTTGGAATAAGTGCGAAGGAGCAGGAAGCAATTTTTAGAGTAGTTGCTGCAATTCTTCATGTCGGAAATATTGAGTTTACCAAGGGCTCGGAAGTTGATTCATCAGTTCCAAAAGATGATAAAGCGAAGTTCCATCTCAAAATGACTGCAGATCTTCTCATGTGCGACCCTGATGCACTGGAAGATGCATTATGTAAGCGTGTGATGATCACACCTGAAGAAGTTATCAAGAGAAGTCTTGATCCTCAAAGTGCAGCAATTAGCCGTGATGGTTTAGCAAAGACAATATATTCTCGATTGTTTGATTGGTTGGTGGACAAAATCAACGTCTCAATTGGGCAAGATGCTAGCTCGAAATCGCTGATTGGGGTCCTTGATATCTATGGTTTTGAAAGTTTTAAGACCAATAGTTTTGAGCAGTTCTGTATTAATTTCACAAACGAGAAACTACAGCAACATTTCAATCAGCATGTCTTCAAGATGGAACAAGAAGAATACACAAAAGAGCAGATTGATTGGAGCTACATTGAGTTTGTCGATAATCAAGACGTTTTGGATCTTATTGAAAAGAAACCTGGTGGAATAGTTGCTCTCCTTGATGAAGCTTGCATGTTTCCAAAGTCGACCCACGAAACATTTGCAAATAAGATTTACCAGACATTCAAAACTCATAAGCGCTTTATAAAGCCAAAATTGTCTCGCACGGATTTTACCATCGCTCATTATGCTGGAGAGGTCCTGTATCAGTCTGACCAATTTTTGGATAAAAACAAGGACTACGTGGTTCCTGAACATCAAGACTTGTTGGGAGCTTCCAAATGTTCCTTTGTGGCAGGCCTTTTCCCTCCACTTCCAGAGGAGACAGCCAAATCTTCCAAGTTTTCTTCGATTGGCTCTCGTTTTAAGCTTCAATTACAATCGTTAATGGAGACCCTAAACTCTACAGAACCTCACTATATTAGATGTGTAAAGCCAAACAATCTTCTAAAACCAGCCGTATTTGAGAACGTCAACATTATGCAGCAACTGCGCTGTGGTGGTGTTTTAGAGGCAATCCGAATTAGTTGTGCTGGCTACCCTACTAGAAAACCATTTTTCGAATTCATAAACAGATTTGGACTTCTTGCACCAGAGGTCTTGGAAGGAAACTATGATGAAAAAGTTGCGTGCACGAAGATACTTGAAAAGAAGGGTCTTAAAGGGTTTCAGATAGGTAAAACAAAGGTATTCCTAAGAGCTGGTCAGATGGCCGAGTTAGATGCACGAAGAGCCGAAGTACTCAGTACTGCAGCTAGAACTATACAGCAGCGTGTACGAACCCATTATGCTAGGAAACGGTTTATTGCACTGCGACAGGCAACCGTAGTTCTGCAATCAATATGCAGAGGAAGCCTGGCTTGCAAAGTCTTCCACGGCATGCAAAAAGAGGCAGCTGCAGTGAAAATTCAGAAGCACCTACGTAAACACCAAGCCAGAACAACTTACAACAAACTCCATGTCTCAGTTCTAGTTGTGCAAACAGGTTTAAGAGCAATGGCCGCTCGTAAAGAATTCAGATTTAAAAGGCAAAGCAAAGCAGCAATTATTATTCAGGCGGTGTGGCGTTGTCATAGAGCTGCCAAATACTTTAAGAGACTCAAGAGGGGTTCAATAGCTGCACAGACCCAAATGAGGGGGAAAATTGCAAGGCGGGAACTTAGGAAGCTCAAAATGGCTGCTAGGGAAACAGGAGCACTTAAAGAAGCAAAAGATGCGCTGGAAAAACGAGTGGAGGAACTAAATTGGCGACTACAGTTGGAAAAACGTTTAAGGACTGACCTAGAAGAAGCTAAAGCACAAGAGATAACGAAATTGAGGAATTCATTGCAAGAAATGCAGACCAAACTTGATGAATCAAATGCATTGATTGTGAAGGAACGAGAGGCTGCAAAGAAAGCTCTTGAAGAGGCACCTCCTGTGGTTCAGGAAACCCAAGTTATGGTTGAGGACACTGCAAAAGTTGAATCTCTAACTGCAGAAGTTGACAGTTTAAAGGCCTCTTTGGAGTCAGAGAAACAAAGAGCAGGAGAAAATGAAACTAAATATAATGAAGCTCAAACATCAGGCGAAGAACGACGCAAGAAATTAGAAGAAACAGAGAAAAAAGTTAGTCAGCTCCAGGAAAATTTGACCAGGCTAGAAGAGAAGCTTACCAATTTAGAATCAGAAAACCAAGTATTACGGCAACAAGCTGTGTCCATGACGCCTAATAAATTCCTTTCTGGACGGTCGAGGTCAATTATTCAGAGGGCAGCTGAAAGTGGCCATATTCCAGGGGATGCAAAGACACCTCTGGATCTGCATAGTCCTTCAGTAAACCACAGGGAGTCGGAATTGGAGGATAAGCCACAGAAATCATTGAATGAAAAACAGCAAGAGAACCAGGAGTTGCTCATTCGATGTATCGCACAACATCTTGGCTTTGCAGGGAACAGGCCTATTGCTGCCTGCATCATATACAAATGCCTTCTGCAGTGGAGATCATTTGAAGTTGAGCGGACTAGCATTTTCGATAGGATTATTCAGACCATAGGGAATGCCATTGAGACCCAGGACAGCAACGATATCTTGGCCTATTGGTTATCCAATGCCTCAACACTTCTATTACTCCTCCAGCGCACACTGAAAGCAAGTGGTGCTGCTGGAATGGCACCCCAACGACGTCGATCATCATCAGCTACTTTGTTTGGAAGGATGACACAAAGTTTCCGTGGAGCCCCGCAAGGTGTCAATCTCTCCTTAATAAATGGTGGAATGAGTGGCGGAGTGGATACTTTACGGCAAGTTGAAGCCAAGTACCCAGCTTTGCTTTTCAAACAGCAACTTACGGCATATGTAGAAAAGATTTATGGGATGATTCGAGATAATTTGAAGAAAGAGATTTCTCCATTGCTTGGATTGTGCATTCAGGCACCAAGAACATCCCGGGCAAGCTTGGTCAAAGGATCGTCGCGATCAGTTGGCAACACTGAGGCCCAGAGAGCTTTGATTGCTCACTGGCAAGGAATCGTTAAGAGCCTCGGAAACTTCTTAAATACTTTAAAAGCAAATTATGTACCACCATTTTTAGTTCGTAAGGTGTTCACGCAAATATTCTCCTTCATCAATGTTCAACTATTCAACAGTCTTCTATTAAGACGAGAATGCTGTTCGTTTAGCAATGGTGAATATGTTAAAGCTGGATTGGCTGAACTGGAGCATTGGTGCTATAAGGCAACAGATGAGTATGCAGGATCAGCTTGGGATGAACTCAAGCATATTCGGCAGGCTATTGGATTCCTGGTCATACATCAAAAACCGAAAAAGACACTTGATGAAATAAGCCATGATCTATGCCCGGTCCTCAGTATACAGCAGCTATATCGTATCAGTACAATGTACTGGGATGACAAATATGGCACGCACAGCGTGTCCTCGGATGTTATATCGAATATGAGAGTGCTAATGACAGAAGATTCCAACAACGCTGTCAGTAATTCTTTCCTGCTGGATGATGATTCCAGCATTCCATTTTCCGTTGATGACATATCAAAGTCGATGAAGCAAATAGACATCTCCGACATTGAACCACCGCCTCTCATCCACGAGAACTCAGGCTTTAGTTTCTTGTTGCCGCGCGCAGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000146 GO:0000166 GO:0000331 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003774 GO:0003779 GO:0003824 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005856 GO:0006928 GO:0006996 GO:0007010 GO:0007015 GO:0008092 GO:0008144 GO:0008150 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0015629 GO:0016043 GO:0016049 GO:0016459 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0022414 GO:0030029 GO:0030036 GO:0030048 GO:0030154 GO:0030554 GO:0030898 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032989 GO:0032991 GO:0033275 GO:0035639 GO:0036094 GO:0040007 GO:0042623 GO:0042641 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043531 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044706 GO:0044877 GO:0048468 GO:0048588 GO:0048589 GO:0048856 GO:0048868 GO:0048869 GO:0051015 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051656 GO:0051704 GO:0060560 GO:0070252 GO:0071840 GO:0097159 GO:0097367 GO:0097435 GO:0097708 GO:0140027 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DIL IQ Myosin_N Myosin_head

Protein Analysis

1530

Amino Acids

172.66

Weight (kDa)

9.01

Isoelectric Point (pI)

43.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Myosin_N	PF02736	9 - 51	1.4e-10	Myosin N-terminal SH3-like domain
Myosin_head	PF00063	64 - 720	4.4e-253	Myosin head (motor domain)
DIL	PF01843	1347 - 1451	1.4e-30	DIL domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005571)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G08730 AT1G08730 AT1G54560 AT1G54560
fragaria_vesca	FvH4_6g02450 FvH4_6g02450
malus_domestica	MD04G1227800.v1.1 MD12G1244600.v1.1
prunus_persica	Prupe.6G347100_v2.0.a1 Prupe.6G347100_v2.0.a1 Prupe.6G347100_v2.0.a1 Prupe.6G347100_v2.0.a1
pyrus_communis	pycom04g20130 pycom04g20140 pycom04g20150 pycom12g22440
rosa_chinensis	RchiOBHm_Chr3g0450241 RchiOBHm_Chr3g0450271
rosa_laevigata	RLG00000025743 RLG00000025747
rosa_multiflora	Rmu_sc0010184.1_g000002
rosa_roxburghii	Rroxscaffold_6G00427950
rosa_rugosa	Rorug02G0626300 Rorug02G0626400
rosa_samantha	Rh3AG025600 Rh3BG026300 Rh3CG025300
rosa_wichuraiana	Rw3G002080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1607
AatII	GACGTC	1 cut(s)	3628
Acc36I	ACCTGC	1 cut(s)	470
Acc65I	GGTACC	1 cut(s)	513
AccB1I	GGYRCC	4 cut(s)	513, 2872, 3613, 3874
AccB7I	CCANNNNNTGG	3 cut(s)	1684, 2902, 3857
AccBSI	CCGCTC	2 cut(s)	2465, 3460
AccI	GTMKAC	2 cut(s)	1547, 4312
AccII	CGCG	4 cut(s)	856, 3916, 4579, 4581
AciI	CCGC	9 cut(s)	511, 2463, 2519, 2614, 3460, 3683, 3726, 4535, 4577
AclI	AACGTT	1 cut(s)	2728
AclWI	GGATC	8 cut(s)	74, 103, 740, 1160, 1488, 3267, 3916, 4204
AcoI	YGGCCR	4 cut(s)	532, 2172, 2460, 3226
AcsI	RAATTY	8 cut(s)	241, 650, 2024, 2367, 2474, 2782, 3088, 3176
AcuI	CTGAAG	3 cut(s)	567, 1161, 3258
AcyI	GRCGYC	2 cut(s)	3167, 3625
AdeI	CACNNNGTG	1 cut(s)	4372
AfaI	GTAC	8 cut(s)	515, 2202, 2209, 2238, 3761, 4020, 4336, 4343
AfeI	AGCGCT	1 cut(s)	1601
AfiI	CCNNNNNNNGG	7 cut(s)	58, 185, 1684, 2595, 2596, 2902, 3857
Alw21I	GWGCWC	3 cut(s)	370, 2096, 2659
Alw26I	GTCTC	9 cut(s)	485, 729, 1258, 1626, 1778, 1847, 2428, 2551, 3503
Alw44I	GTGCAC	1 cut(s)	2092
AlwI	GGATC	8 cut(s)	74, 103, 740, 1160, 1488, 3267, 3916, 4204
AlwNI	CAGNNNCTG	1 cut(s)	1951
Ama87I	CYCGRG	1 cut(s)	3888
Aor51HI	AGCGCT	1 cut(s)	1601
AoxI	GGCC	9 cut(s)	532, 1759, 2172, 2460, 2958, 3226, 3398, 3534, 3936
ApaLI	GTGCAC	1 cut(s)	2092
ApoI	RAATTY	8 cut(s)	241, 650, 2024, 2367, 2474, 2782, 3088, 3176
ArsI	GACNNNNNNTTYG	4 cut(s)	1236, 1268, 2406, 2438
AseI	ATTAAT	1 cut(s)	1353
Asp718I	GGTACC	1 cut(s)	513
AspLEI	GCGC	7 cut(s)	858, 1602, 1956, 2680, 3582, 4581, 4583
AspS9I	GGNCC	4 cut(s)	1294, 1660, 3937, 4303
AsuC2I	CCSGG	4 cut(s)	536, 3889, 3890, 4301
AsuHPI	GGTGA	2 cut(s)	479, 4139
AsuII	TTCGAA	1 cut(s)	2022
AsuNHI	GCTAGC	1 cut(s)	1271
AvaI	CYCGRG	1 cut(s)	3888
AvaII	GGWCC	3 cut(s)	1294, 1660, 4303
BaeGI	GKGCMC	1 cut(s)	2096
BalI	TGGCCA	1 cut(s)	3228
BanI	GGYRCC	4 cut(s)	513, 2872, 3613, 3874
BanII	GRGCYC	3 cut(s)	389, 1013, 3682
BauI	CACGAG	2 cut(s)	726, 4547
BbsI	GAAGAC	4 cut(s)	54, 1390, 2323, 4082
Bbv12I	GWGCWC	3 cut(s)	370, 2096, 2659
BccI	CCATC	8 cut(s)	127, 332, 336, 1065, 1187, 1399, 1646, 2164
BceAI	ACGGC	6 cut(s)	1173, 1904, 2353, 3161, 3758, 3807
BcgI	CGANNNNNNTGC	8 cut(s)	1079, 1113, 1544, 1578, 2165, 2199, 4307, 4341
BciVI	GTATCC	1 cut(s)	3727
BclI	TGATCA	2 cut(s)	429, 1131
BcnI	CCSGG	4 cut(s)	536, 3889, 3890, 4301
BcoDI	GTCTC	9 cut(s)	485, 729, 1258, 1626, 1778, 1847, 2428, 2551, 3503
BfmI	CTRYAG	9 cut(s)	236, 1071, 1373, 1866, 2211, 2358, 2714, 2937, 3434
BfoI	RGCGCY	1 cut(s)	1603
BfuAI	ACCTGC	1 cut(s)	470
BfuI	GTATCC	1 cut(s)	3727
BglII	AGATCT	1 cut(s)	1075
BmcAI	AGTACT	2 cut(s)	2202, 2209
Bme18I	GGWCC	3 cut(s)	1294, 1660, 4303
BmeT110I	CYCGRG	1 cut(s)	3888
BmgT120I	GGNCC	4 cut(s)	1294, 1660, 3937, 4303
BmiI	GGNNCC	7 cut(s)	515, 1295, 1710, 2874, 3615, 3679, 3876
BmrI	ACTGGG	1 cut(s)	4354
BmsI	GCATC	9 cut(s)	339, 874, 1087, 1099, 1258, 2173, 2665, 3232, 3423
BmtI	GCTAGC	1 cut(s)	1275
BmuI	ACTGGG	1 cut(s)	4354
BoxI	GACNNNNGTC	1 cut(s)	79
BpiI	GAAGAC	4 cut(s)	54, 1390, 2323, 4082
BpmI	CTGGAG	5 cut(s)	249, 1674, 3065, 3560, 4178
Bpu14I	TTCGAA	1 cut(s)	2022
BpuEI	CTTGAG	2 cut(s)	2546, 4199
BpuMI	CCSGG	4 cut(s)	536, 3889, 3890, 4301
BsaAI	YACGTR	2 cut(s)	1705, 2384
BsaBI	GATNNNNATC	1 cut(s)	3634
BsaHI	GRCGYC	2 cut(s)	3167, 3625
BsaI	GGTCTC	4 cut(s)	485, 729, 1847, 3503
BsaJI	CCNNGG	9 cut(s)	112, 1005, 2335, 3236, 3513, 3673, 3888, 3982, 4377
BsaXI	ACNNNNNCTCC	2 cut(s)	4151, 4181
Bsc4I	CCNNNNNNNGG	7 cut(s)	58, 185, 1684, 2595, 2596, 2902, 3857
Bse3DI	GCAATG	8 cut(s)	127, 432, 613, 763, 2463, 2786, 3851, 4129
Bse8I	GATNNNNATC	1 cut(s)	3634
BseDI	CCNNGG	9 cut(s)	112, 1005, 2335, 3236, 3513, 3673, 3888, 3982, 4377
BseGI	GGATG	9 cut(s)	3247, 3663, 3822, 3884, 4213, 4354, 4387, 4459, 4542
BseJI	GATNNNNATC	1 cut(s)	3634
BseLI	CCNNNNNNNGG	7 cut(s)	58, 185, 1684, 2595, 2596, 2902, 3857
BseMI	GCAATG	8 cut(s)	127, 432, 613, 763, 2463, 2786, 3851, 4129
BseMII	CTCAG	8 cut(s)	96, 480, 702, 2218, 2439, 3924, 4321, 4569
BsePI	GCGCGC	1 cut(s)	4579
BseRI	GAGGAG	2 cut(s)	1796, 3563
BseSI	GKGCMC	1 cut(s)	2096
BseYI	CCCAGC	1 cut(s)	3763
BsgI	GTGCAG	3 cut(s)	771, 1197, 2565
Bsh1236I	CGCG	4 cut(s)	856, 3916, 4579, 4581
Bsh1285I	CGRYCG	1 cut(s)	3194
BshFI	GGCC	9 cut(s)	534, 1761, 2174, 2462, 2960, 3228, 3400, 3536, 3938
BshNI	GGYRCC	4 cut(s)	513, 2872, 3613, 3874
BsiEI	CGRYCG	1 cut(s)	3194
BsiHKAI	GWGCWC	3 cut(s)	370, 2096, 2659
BsiHKCI	CYCGRG	1 cut(s)	3888
BsiSI	CCGG	4 cut(s)	186, 535, 3889, 4301
BslI	CCNNNNNNNGG	7 cut(s)	58, 185, 1684, 2595, 2596, 2902, 3857
BsmAI	GTCTC	9 cut(s)	485, 729, 1258, 1626, 1778, 1847, 2428, 2551, 3503
BsmBI	CGTCTC	1 cut(s)	1258
BsmI	GAATGC	4 cut(s)	3505, 3867, 4112, 4467
BsnI	GGCC	9 cut(s)	534, 1761, 2174, 2462, 2960, 3228, 3400, 3536, 3938
Bso31I	GGTCTC	4 cut(s)	485, 729, 1847, 3503
BsoBI	CYCGRG	1 cut(s)	3888
Bsp119I	TTCGAA	1 cut(s)	2022
Bsp1286I	GDGCHC	6 cut(s)	370, 389, 1013, 2096, 2659, 3682
Bsp68I	TCGCGA	1 cut(s)	3916
BspACI	CCGC	9 cut(s)	511, 2463, 2519, 2614, 3460, 3683, 3726, 4535, 4577
BspANI	GGCC	9 cut(s)	534, 1761, 2174, 2462, 2960, 3228, 3400, 3536, 3938
BspCNI	CTCAG	8 cut(s)	97, 481, 701, 2217, 2438, 3925, 4320, 4568
BspFNI	CGCG	4 cut(s)	856, 3916, 4579, 4581
BspLI	GGNNCC	7 cut(s)	515, 1295, 1710, 2874, 3615, 3679, 3876
BspMAI	CTGCAG	6 cut(s)	240, 1075, 2215, 2362, 2941, 3438
BspMI	ACCTGC	1 cut(s)	470
BspOI	GCTAGC	1 cut(s)	1275
BspPI	GGATC	8 cut(s)	74, 103, 740, 1160, 1488, 3267, 3916, 4204
BspQI	GCTCTTC	1 cut(s)	2185
BspT104I	TTCGAA	1 cut(s)	2022
BspT107I	GGYRCC	4 cut(s)	513, 2872, 3613, 3874
BspTNI	GGTCTC	4 cut(s)	485, 729, 1847, 3503
BsrBI	CCGCTC	2 cut(s)	2465, 3460
BsrDI	GCAATG	8 cut(s)	127, 432, 613, 763, 2463, 2786, 3851, 4129
BssECI	CCNNGG	9 cut(s)	112, 1005, 2335, 3236, 3513, 3673, 3888, 3982, 4377
BssHII	GCGCGC	1 cut(s)	4579
BssNAI	GTATAC	1 cut(s)	4313
BssNI	GRCGYC	2 cut(s)	3167, 3625
BssSI	CACGAG	2 cut(s)	726, 4547
BssT1I	CCWWGG	1 cut(s)	1005
Bst1107I	GTATAC	1 cut(s)	4313
Bst2BI	CACGAG	2 cut(s)	726, 4547
Bst4CI	ACNGT	9 cut(s)	559, 619, 723, 2262, 2287, 2718, 2951, 3192, 4088
Bst6I	CTCTTC	3 cut(s)	2185, 2862, 3099
BstACI	GRCGYC	2 cut(s)	3167, 3625
BstAPI	GCANNNNNTGC	5 cut(s)	413, 1951, 2675, 2797, 3599
BstBAI	YACGTR	2 cut(s)	1705, 2384
BstBI	TTCGAA	1 cut(s)	2022
BstDSI	CCRYGG	2 cut(s)	2335, 3673
BstF5I	GGATG	9 cut(s)	3247, 3663, 3822, 3884, 4213, 4354, 4387, 4459, 4542
BstFNI	CGCG	4 cut(s)	856, 3916, 4579, 4581
BstH2I	RGCGCY	1 cut(s)	1603
BstHHI	GCGC	7 cut(s)	858, 1602, 1956, 2680, 3582, 4581, 4583
BstMAI	GTCTC	9 cut(s)	485, 729, 1258, 1626, 1778, 1847, 2428, 2551, 3503
BstMCI	CGRYCG	1 cut(s)	3194
BstNSI	RCATGY	3 cut(s)	1397, 1537, 2344
BstPAI	GACNNNNGTC	1 cut(s)	79
BstSFI	CTRYAG	9 cut(s)	236, 1071, 1373, 1866, 2211, 2358, 2714, 2937, 3434
BstSLI	GKGCMC	1 cut(s)	2096
BstSNI	TACGTA	1 cut(s)	2384
BstUI	CGCG	4 cut(s)	856, 3916, 4579, 4581
BstV2I	GAAGAC	4 cut(s)	54, 1390, 2323, 4082
BstX2I	RGATCY	4 cut(s)	108, 1075, 1480, 3259
BstYI	RGATCY	4 cut(s)	108, 1075, 1480, 3259
BstZ17I	GTATAC	1 cut(s)	4313
BsuI	GTATCC	1 cut(s)	3727
BsuRI	GGCC	9 cut(s)	534, 1761, 2174, 2462, 2960, 3228, 3400, 3536, 3938
BtgI	CCRYGG	2 cut(s)	2335, 3673
BtgZI	GCGATG	1 cut(s)	1624
BtsCI	GGATG	9 cut(s)	3247, 3663, 3822, 3884, 4213, 4354, 4387, 4459, 4542
BtsI	GCAGTG	4 cut(s)	2270, 2367, 2913, 3443
BtuMI	TCGCGA	1 cut(s)	3916
BveI	ACCTGC	1 cut(s)	470
CaiI	CAGNNNCTG	1 cut(s)	1951
CfoI	GCGC	7 cut(s)	858, 1602, 1956, 2680, 3582, 4581, 4583
Cfr13I	GGNCC	4 cut(s)	1294, 1660, 3937, 4303
Cfr9I	CCCGGG	1 cut(s)	3888
CseI	GACGC	2 cut(s)	3048, 3175
CsiI	ACCWGGT	1 cut(s)	1498
Csp6I	GTAC	8 cut(s)	514, 2201, 2208, 2237, 3760, 4019, 4335, 4342
CviQI	GTAC	8 cut(s)	514, 2201, 2208, 2237, 3760, 4019, 4335, 4342
DraI	TTTAAA	4 cut(s)	246, 2485, 2955, 4005
DraIII	CACNNNGTG	1 cut(s)	4372
EaeI	YGGCCR	4 cut(s)	532, 2172, 2460, 3226
Eam1104I	CTCTTC	3 cut(s)	2185, 2862, 3099
EarI	CTCTTC	3 cut(s)	2185, 2862, 3099
EciI	GGCGGA	1 cut(s)	3741
Eco105I	TACGTA	1 cut(s)	2384
Eco130I	CCWWGG	1 cut(s)	1005
Eco147I	AGGCCT	3 cut(s)	1761, 2960, 3400
Eco24I	GRGCYC	3 cut(s)	389, 1013, 3682
Eco31I	GGTCTC	4 cut(s)	485, 729, 1847, 3503
Eco32I	GATATC	2 cut(s)	1303, 3529
Eco47I	GGWCC	3 cut(s)	1294, 1660, 4303
Eco47III	AGCGCT	1 cut(s)	1601
Eco57I	CTGAAG	3 cut(s)	567, 1161, 3258
Eco88I	CYCGRG	1 cut(s)	3888
EcoO109I	RGGNCCY	2 cut(s)	1294, 1660
EcoRI	GAATTC	3 cut(s)	2024, 2474, 2782
EcoRV	GATATC	2 cut(s)	1303, 3529
EcoT14I	CCWWGG	1 cut(s)	1005
EcoT22I	ATGCAT	2 cut(s)	1114, 2827
EcoT38I	GRGCYC	3 cut(s)	389, 1013, 3682
ErhI	CCWWGG	1 cut(s)	1005
Esp3I	CGTCTC	1 cut(s)	1258
FalI	AAGNNNNNCTT	4 cut(s)	2305, 2337, 3096, 3128
FauI	CCCGC	2 cut(s)	2607, 3690
FauNDI	CATATG	1 cut(s)	3796
FbaI	TGATCA	2 cut(s)	429, 1131
FblI	GTMKAC	2 cut(s)	1547, 4312
FokI	GGATG	9 cut(s)	3254, 3670, 3829, 3871, 4220, 4361, 4394, 4466, 4529
FriOI	GRGCYC	3 cut(s)	389, 1013, 3682
GlaI	GCGC	7 cut(s)	857, 1601, 1955, 2679, 3581, 4580, 4582
GsaI	CCCAGC	1 cut(s)	3767
GsuI	CTGGAG	5 cut(s)	249, 1674, 3065, 3560, 4178
HaeII	RGCGCY	1 cut(s)	1603
HaeIII	GGCC	9 cut(s)	534, 1761, 2174, 2462, 2960, 3228, 3400, 3536, 3938
HapII	CCGG	4 cut(s)	186, 535, 3889, 4301
HgaI	GACGC	2 cut(s)	3048, 3175
HhaI	GCGC	7 cut(s)	858, 1602, 1956, 2680, 3582, 4581, 4583
Hin1I	GRCGYC	2 cut(s)	3167, 3625
Hin6I	GCGC	7 cut(s)	856, 1600, 1954, 2678, 3580, 4579, 4581
HinP1I	GCGC	7 cut(s)	856, 1600, 1954, 2678, 3580, 4579, 4581
HincII	GTYRAC	4 cut(s)	193, 1548, 1936, 2947
HindII	GTYRAC	4 cut(s)	193, 1548, 1936, 2947
HindIII	AAGCTT	4 cut(s)	1527, 1829, 3110, 3895
HpaII	CCGG	4 cut(s)	186, 535, 3889, 4301
HphI	GGTGA	2 cut(s)	479, 4139
Hpy99I	CGWCG	5 cut(s)	74, 3042, 3627, 3630, 3916
HpyCH4III	ACNGT	9 cut(s)	559, 619, 723, 2262, 2287, 2718, 2951, 3192, 4088
HpyCH4IV	ACGT	7 cut(s)	1251, 1473, 1704, 1932, 2383, 2728, 3625
HpySE526I	ACGT	7 cut(s)	1251, 1473, 1704, 1932, 2383, 2728, 3625
Hsp92I	GRCGYC	2 cut(s)	3167, 3625
HspAI	GCGC	7 cut(s)	856, 1600, 1954, 2678, 3580, 4579, 4581
KpnI	GGTACC	1 cut(s)	517
Ksp22I	TGATCA	2 cut(s)	429, 1131
LguI	GCTCTTC	1 cut(s)	2185
LweI	GCATC	9 cut(s)	339, 874, 1087, 1099, 1258, 2173, 2665, 3232, 3423
MabI	ACCWGGT	1 cut(s)	1498
MaeII	ACGT	7 cut(s)	1251, 1473, 1704, 1932, 2383, 2728, 3625
MaeIII	GTNAC	1 cut(s)	341
MbiI	CCGCTC	2 cut(s)	2465, 3460
MfeI	CAATTG	1 cut(s)	1257
MflI	RGATCY	4 cut(s)	108, 1075, 1480, 3259
MhlI	GDGCHC	6 cut(s)	370, 389, 1013, 2096, 2659, 3682
MlsI	TGGCCA	1 cut(s)	3228
MluNI	TGGCCA	1 cut(s)	3228
MlyI	GAGTC	3 cut(s)	2553, 2978, 3299
MmeI	TCCRAC	6 cut(s)	907, 967, 2700, 3273, 4446, 4545
Mox20I	TGGCCA	1 cut(s)	3228
Mph1103I	ATGCAT	2 cut(s)	1114, 2827
MscI	TGGCCA	1 cut(s)	3228
MslI	CAYNNNNRTG	2 cut(s)	333, 500
Msp20I	TGGCCA	1 cut(s)	3228
MspA1I	CMGCKG	2 cut(s)	2357, 3218
MspI	CCGG	4 cut(s)	186, 535, 3889, 4301
MunI	CAATTG	1 cut(s)	1257
Mva1269I	GAATGC	4 cut(s)	3505, 3867, 4112, 4467
MvnI	CGCG	4 cut(s)	856, 3916, 4579, 4581
NciI	CCSGG	4 cut(s)	536, 3889, 3890, 4301
NdeI	CATATG	1 cut(s)	3796
NheI	GCTAGC	1 cut(s)	1271
NlaIV	GGNNCC	7 cut(s)	515, 1295, 1710, 2874, 3615, 3679, 3876
NmeAIII	GCCGAG	1 cut(s)	2200
NmuCI	GTSAC	1 cut(s)	341
NruI	TCGCGA	1 cut(s)	3916
NsiI	ATGCAT	2 cut(s)	1114, 2827
NspI	RCATGY	3 cut(s)	1397, 1537, 2344
NspV	TTCGAA	1 cut(s)	2022
PaeI	GCATGC	1 cut(s)	2344
PauI	GCGCGC	1 cut(s)	4579
PceI	AGGCCT	3 cut(s)	1761, 2960, 3400
PciSI	GCTCTTC	1 cut(s)	2185
PctI	GAATGC	4 cut(s)	3505, 3867, 4112, 4467
PflMI	CCANNNNNTGG	3 cut(s)	1684, 2902, 3857
PfoI	TCCNGGA	1 cut(s)	3081
PleI	GAGTC	3 cut(s)	2553, 2977, 3298
PpsI	GAGTC	3 cut(s)	2553, 2977, 3298
Ppu21I	YACGTR	2 cut(s)	1705, 2384
PpuMI	RGGWCCY	2 cut(s)	1294, 1660
PshAI	GACNNNNGTC	1 cut(s)	79
PshBI	ATTAAT	1 cut(s)	1353
PsiI	TTATAA	1 cut(s)	1607
Psp1406I	AACGTT	1 cut(s)	2728
Psp5II	RGGWCCY	2 cut(s)	1294, 1660
PspFI	CCCAGC	1 cut(s)	3763
PspN4I	GGNNCC	7 cut(s)	515, 1295, 1710, 2874, 3615, 3679, 3876
PspPI	GGNCC	4 cut(s)	1294, 1660, 3937, 4303
PspPPI	RGGWCCY	2 cut(s)	1294, 1660
PstI	CTGCAG	6 cut(s)	240, 1075, 2215, 2362, 2941, 3438
PstNI	CAGNNNCTG	1 cut(s)	1951
PsuI	RGATCY	4 cut(s)	108, 1075, 1480, 3259
PteI	GCGCGC	1 cut(s)	4579
PvuII	CAGCTG	2 cut(s)	2357, 3218
RruI	TCGCGA	1 cut(s)	3916
RsaI	GTAC	8 cut(s)	515, 2202, 2209, 2238, 3761, 4020, 4336, 4343
RsaNI	GTAC	8 cut(s)	514, 2201, 2208, 2237, 3760, 4019, 4335, 4342
RseI	CAYNNNNRTG	2 cut(s)	333, 500
SalI	GTCGAC	1 cut(s)	1546
SapI	GCTCTTC	1 cut(s)	2185
Sau96I	GGNCC	4 cut(s)	1294, 1660, 3937, 4303
ScaI	AGTACT	2 cut(s)	2202, 2209
SchI	GAGTC	3 cut(s)	2553, 2978, 3299
SduI	GDGCHC	6 cut(s)	370, 389, 1013, 2096, 2659, 3682
SexAI	ACCWGGT	1 cut(s)	1498
SfaNI	GCATC	9 cut(s)	339, 874, 1087, 1099, 1258, 2173, 2665, 3232, 3423
SfcI	CTRYAG	9 cut(s)	236, 1071, 1373, 1866, 2211, 2358, 2714, 2937, 3434
SfuI	TTCGAA	1 cut(s)	2022
SinI	GGWCC	3 cut(s)	1294, 1660, 4303
SmaI	CCCGGG	1 cut(s)	3890
SmiMI	CAYNNNNRTG	2 cut(s)	333, 500
SmlI	CTYRAG	2 cut(s)	2561, 4214
SmoI	CTYRAG	2 cut(s)	2561, 4214
SnaBI	TACGTA	1 cut(s)	2384
SphI	GCATGC	1 cut(s)	2344
SseBI	AGGCCT	3 cut(s)	1761, 2960, 3400
SsiI	CCGC	9 cut(s)	511, 2463, 2519, 2614, 3460, 3683, 3726, 4535, 4577
SspI	AATATT	2 cut(s)	994, 4056
StuI	AGGCCT	3 cut(s)	1761, 2960, 3400
StyI	CCWWGG	1 cut(s)	1005
TaaI	ACNGT	9 cut(s)	559, 619, 723, 2262, 2287, 2718, 2951, 3192, 4088
TaiI	ACGT	7 cut(s)	1254, 1476, 1707, 1935, 2386, 2731, 3628
TatI	WGTACW	4 cut(s)	2200, 2207, 4334, 4341
TauI	GCSGC	2 cut(s)	2465, 4579
TseFI	GTSAC	1 cut(s)	341
Tsp45I	GTSAC	1 cut(s)	341
TspGWI	ACGGA	6 cut(s)	87, 568, 769, 1643, 3662, 4468
TspMI	CCCGGG	1 cut(s)	3888
Van91I	CCANNNNNTGG	3 cut(s)	1684, 2902, 3857
VneI	GTGCAC	1 cut(s)	2092
VpaK11BI	GGWCC	3 cut(s)	1294, 1660, 4303
VspI	ATTAAT	1 cut(s)	1353
XapI	RAATTY	8 cut(s)	241, 650, 2024, 2367, 2474, 2782, 3088, 3176
XceI	RCATGY	3 cut(s)	1397, 1537, 2344
XcmI	CCANNNNNNNNNTGG	3 cut(s)	60, 119, 335
XmaI	CCCGGG	1 cut(s)	3888
XmiI	GTMKAC	2 cut(s)	1547, 4312
ZraI	GACGTC	1 cut(s)	3626
ZrmI	AGTACT	2 cut(s)	2202, 2209
Zsp2I	ATGCAT	2 cut(s)	1114, 2827

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.