Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0005571

Physical Location & Seq

Genomic Coordinates Reverse (-)

31535180 .. 31544793

Gene Structure 9614 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1244600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4671 bp

ATGGTTATCAGTGAATTTTGTGGAACACCAGTGAATATCATTGTAGGTTCTCATGTCTGGGTTGAAGACTCAGAATTGGCCTGGATTGATGGCCAAGTGTCAAAGATTACTGGACAAGAAGCTGAGATCGAGAGTAGCAATGGGAAGAAGATGGTTGTCAAATTGGCGAAAATATATCCAAAAGATATGGAAGCTCCTGCTGCTGGAGTTGATGACATGACAAAGCTATCTTATCTGCATGAGCCTGGAGTTTTGCAGAATTTAAAAACTAGATACGAACTTAATGAAATCTATACTTATACTGGAAATATCCTTATTGCCATTAATCCATTCCAAAGACTGCCGCATCTCTACGATGGTCACATGATGCAACAATACAAGGGCGCGCCATTTGGAGAACTAAATCCTCATGTTTTTGCAGTTGCGGACGTTGCATATAGGGCAATGATCAATGAGGGAAAAAGCAATTCAATTCTGGTCAGTGGTGAAAGTGGAGCAGGTAAAACTGAGACCACAAAAATGCTTATGAGGTACCTTGCATTTCTAGGTGGCCGGGCTGCAACTGAAGGACGGACAGTTGAACAGCAAGTTCTTGAATCAAATCCAGTTCTGGAAGCATTTGGCAACGCTAAAACTGTTAGAAATAACAATTCTAGGCAAGACGATATCAAGCTAGTACATATTTGTCAAGGAAGAATCTCAGGAGCAGCCATTAGAACCTACCTTCTGGAACGGTCTCGTGTTTGCCAAGTTTCTGATCCTGAACGGAACTACCACTGCTTCTACCTTCTTTGTGCTGCACCACAGGAGGAAATTGAGAAATACAAGTTGGCAAATCCTAAATCATTTCACTATCTTAACCAGTCACGTTGCTACGAACTGGTTGGTGTAAGTGATGCTCATGACTATCTTGCCACAAGGAGAGCTATGGATATTGTTGGAATAAGTGCTAAGGAGCAGGAAGCAATTTTTAGAGTAGTTGCTGCAATTCTTCATCTAGGAAATATTGAGTTTTCCAAGGGCTCGGAAGTTGATTCGTCAGTTCCAAAAGATGATAAAGCAAAGTTCCATCTTAAGATGACTTCAGAGCTTCTCATGTGTGACCCTGATGCATTGGAAGATGCATTATGTAAGCGTGTCATGATCACACCTGAAGAAGTTATCAAGAGAAGTCTTGATCCTCAAAGTGCAGCAATTAGCCGCGATGGTTTAGCAAAGACAGTATATTCTCGATTGTTTGATTGGTTGGTGGATAAAATTAATGTCTCAATTGGACAAGATGCTACCTCGAAATCGCTGATTGGGGTCCTTGATATCTATGGTTTTGAAAGTTTTAAGACCAATAGTTTTGAGCAGTTCTGCATTAATTTCACAAACGAGAAATTGCAGCAACATTTCAATCAGCTCTTGGGTTATTGTTCCTGTTGCCGACCCCACTTAGTGGGAAAAGGCTTTGTTGTTGTTGTTGTTTGGCACGTCTTCAAGATGGAACAAGAAGAATACACAAAAGAGCAGATTGATTGGAGCTACATTGAGTTTGTCGATAATCAAGACGTTTTGGATCTTATTGAAAAGAAACCTGGCGGAATAGTTGCTCTCCTTGATGAAGCTTGCATGTTTCCCAAGTCAACCCATGAAACATTTGCAAATAAGCTTTATCAGACATTCAAAACTCACAAGCGCTTTATAAAGCCAAAACTTTCTCGCACGGATTTTACCATCGCTCATTATGCTGGAGAGGTCCAATATCAATCTGACCAATTTTTGGACAAAAACAAGGACTATGTGGTTCCTGAACATCAAGATTTGTTGGGTGTTTCCAAATGTCCTTTCGTGGCAGGCCTTTTCCCTCCACTTCCAGAGGAGACAGCCAAATCTTCCAAGTTTTCTTCGATTGGCTCTCGTTTTAAGCTTCAACTACAATCGTTAATGGAAACCCTAAACTCTACAGAACCTCACTATGTCAGATGTGTAAAGCCAAATAATTTTCTAAAACCAGCCGTGTTTGAGAACGTCAACATTATGCAGCAACTGCGCTGTGGTGGTGTTTTAGAGGCAATCCGTATTAGCTGCGCTGGCTACCCTACTCGAAAACCCTTTTTCGAATTCATAAACAGATTTGGACTTCTTGCACCAGAGGTCTTGGAAGGAAACTACGACGAAAAGGTTGCATGCACTAGGATTCTCGAAAAGAAGGGTCTTAAAGGGTTTCAGATAGGTAAAACAAAGGTATTCCTGAGGGCTGGTCAGATGGCTGAGTTAGACGCACGAAGAACCGAAGTACTCAGTACTGCAGCTAAAACTATACAGCGGCGTGTACGAACCCATTATGCTAGGAAACGGTTTATTGCACTGCGACAGGCAACCATAGTTATGCAATCGATATGCAGAGGAAGCCTGGCTGGCAAAGTCTTCCACGGCATGAAAAGAGAGTCAGCTGCCGTGAAAATTCAGAAGAACCTACGTAAACACCAAGCCAGAACAACTTACAACAAGCTCCATGTCTCAGTTCTTGTCGTGCAGACAGGTTTAAGAGCAATGGCTGGACGTAAAGAATTCAGATTTAAAAGGCAAACCAAAGCAGCAACCGTTGTCCAGGCGGTGTGGCGTTGTCATAAAGCTGCCAAATACTTTAAGAGGCTCAAGAGGGGTTCAGTAGTCGCACAGTGCCAAATGAGGGGGAAAATCGCAAGGAAGGAACTTAGGAAGCTAAAAATGGCTGCAAGGGAAACAGGAGCACTTAAGGAGGCAAAAGATGCACTGGAAAAACGAGTGGAGGAACTAAATTGGCGACTACAGTTGGAAAAACGTTTAAGGACTGACCTAGAAGAAGCAAAGGCACAAGAGATAACGAAATTGCAGAACTCATTGCAAGAAATGCAGACCAAACTTGATGAATCAAACGCATTGATTGTGAAGGAACAAGAGGCTGCAAAGAAAGCTATTGAAGAGGCTCCTCCTGTGGTTCAGGAAACCCAAGTTATGGTCGAGGACACTGAAAAAGTTGAATCTCTAACAGCAGAAGTTGACAGTTTAAAGGCCTCTATGGAGTCAGAGAAAAAAAGAGCAGAAGAAAATGAAAGAAAATATAATGAAGCTCAAACATCGGGCGAAGAACGACGCAAAAAATTAGAAGAAACAGAGAAAAAAGTTAGTCAGCTCCAGGAAAATTTGACCAGGCTAGAAGAGAAGCTTGCCAATTTAGAATCAGAAAATCAAGTTTTACGCCAACAAGCTGTGTCCATGACGCCTAATAAATTCCTTTCTGGACGGTCCAGATCAACTATTCAGAGGGCAGCTGAAAGTAGTCATATTCCAGGGGATGCAAAGACAGCTCTGGAAATGCATAGTCCTTCAATAAATCACAGGGAGTCGGAATTGGAGGATAAGCCACAGAAATCACTAAATGAAAAACAGCAAGAGAACCAGGAGTTGCTCATTCGATGCATTGCACAACACCTGGGCTTTGCAGGGAACAGGCCTATTGCTGCCTGCATCATATACAAATGCCTTTTGCAGTGGAGATCATTCGAAGTTGAGCGGACTAGTATTTTCGATAGGATTATTCAGACCATAGGGAATGCCATTGAGACCCAGGATAACAACGATATCTTGGCCTATTGGCTATCCAACGCCTCAACACTTCTATTACTACTCCAGCGCACACTGAAAGCAAGCGGTGCTGCTGGAATGGCACCCCAACGACGTCGATCATCATCAGCTACTCTGTTTGGAAGGATGACACAAAGTTTCCGTGGAGCGCCACAAGGTGTTAATATTTCCTTAATCAATGGTGGAATGAGTGGCGGAGTGGACTCTTTACGGCAAGTTGAAGCCAAGTACCCAGCTTTGCTTTTCAAACAGCAACTTACAGCATATGTAGAAAAAATTTACGGGATGATTCGAGATAATTTGAAGAAAGAGATTTCTCCTTTGCTTGGATTGTGCATCCAGGCACCAAGAACATCCAGGGCAAGCTTGGTCAAAGGATCGTCGCGATCAGTTGCCAACACTGAGGCCCAGAAAGCTTTGATTGCTCACTGGCAGGGGATTGTCAAGAGCCTCGGAAACTTCTTAAATACTTTAAAAGCAAATCATGTACCACCATTTTTAGTTCGTAAGGTGTTCACGCAAATATTCTCCTTCATCAATGTTCAGCTATTCAACAGTCTTCTATTAAGACGAGAATGCTGTTCGTTTAGTAATGGTGAATATGTTAAAGCTGGGTTGGCTGAACTGGAGCATTGGTGCTATAAGGCAACAGATGAGTATGCAGGATCAGCTTGGGATGAACTCAAGCATATTCGGCAGGCTATTGGATTCCTGGTCATACATCAAAAACCGAAAAAGACGCTTGATGAAATTAGCCATGACCTATGCCCGGTCCTCAGTATACAGCAGCTATATCGTATCAGTACAATGTACTGGGATGACAAATATGGCACGCATAGTGTGTCCTCGGATGTTATATCGAATATGAGAGTGCTGATGACAGAAGATTCCAACAACGCAGTCAGTAATTCTTTCCTGCTGGATGACGATTCCAGCATTCCATTCTCCGTTGACGACATATCAAAGTCGATGGAGCAAATAGACATCTCCGACATTGAACCACCACCGCTCATTCACGAGAACTCAGGCTTTAGTTTCTTGTTGCCGCGCGCAGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000146 GO:0000166 GO:0000331 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003774 GO:0003779 GO:0003824 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005856 GO:0006928 GO:0006996 GO:0007010 GO:0007015 GO:0008092 GO:0008144 GO:0008150 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0015629 GO:0016043 GO:0016049 GO:0016459 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0022414 GO:0030029 GO:0030036 GO:0030048 GO:0030154 GO:0030554 GO:0030898 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032989 GO:0032991 GO:0033275 GO:0035639 GO:0036094 GO:0040007 GO:0042623 GO:0042641 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043531 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044706 GO:0044877 GO:0048468 GO:0048588 GO:0048589 GO:0048856 GO:0048868 GO:0048869 GO:0051015 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051656 GO:0051704 GO:0060560 GO:0070252 GO:0071840 GO:0097159 GO:0097367 GO:0097435 GO:0097708 GO:0140027 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DIL IQ Myosin_N Myosin_head

Protein Analysis

1557

Amino Acids

175.86

Weight (kDa)

8.86

Isoelectric Point (pI)

46.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Myosin_N	PF02736	15 - 57	2.9e-09	Myosin N-terminal SH3-like domain
Myosin_head	PF00063	70 - 469	3.1e-151	Myosin head (motor domain)
Myosin_head	PF00063	492 - 747	6e-80	Myosin head (motor domain)
DIL	PF01843	1374 - 1478	1.4e-30	DIL domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005571)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G08730 AT1G08730 AT1G54560 AT1G54560
fragaria_vesca	FvH4_6g02450 FvH4_6g02450
malus_domestica	MD04G1227800.v1.1 MD12G1244600.v1.1
prunus_persica	Prupe.6G347100_v2.0.a1 Prupe.6G347100_v2.0.a1 Prupe.6G347100_v2.0.a1 Prupe.6G347100_v2.0.a1
pyrus_communis	pycom04g20130 pycom04g20140 pycom04g20150 pycom12g22440
rosa_chinensis	RchiOBHm_Chr3g0450241 RchiOBHm_Chr3g0450271
rosa_laevigata	RLG00000025743 RLG00000025747
rosa_multiflora	Rmu_sc0010184.1_g000002
rosa_roxburghii	Rroxscaffold_6G00427950
rosa_rugosa	Rorug02G0626300 Rorug02G0626400
rosa_samantha	Rh3AG025600 Rh3BG026300 Rh3CG025300
rosa_wichuraiana	Rw3G002080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1688
AatII	GACGTC	1 cut(s)	3709
Acc36I	ACCTGC	1 cut(s)	488
Acc65I	GGTACC	1 cut(s)	531
AccB1I	GGYRCC	3 cut(s)	531, 3694, 3955
AccB7I	CCANNNNNTGG	3 cut(s)	1441, 1765, 2983
AccBSI	CCGCTC	2 cut(s)	3541, 4621
AccI	GTMKAC	1 cut(s)	4393
AccII	CGCG	5 cut(s)	386, 1203, 3997, 4660, 4662
AclI	AACGTT	1 cut(s)	2809
AclWI	GGATC	5 cut(s)	752, 1172, 1569, 3997, 4285
AcoI	YGGCCR	2 cut(s)	91, 550
AcsI	RAATTY	8 cut(s)	14, 259, 2105, 2448, 2555, 3169, 3257, 3888
AcuI	CTGAAG	3 cut(s)	585, 1068, 1173
AcyI	GRCGYC	2 cut(s)	3248, 3706
AdeI	CACNNNGTG	2 cut(s)	1441, 3770
AfaI	GTAC	9 cut(s)	533, 678, 2283, 2290, 2319, 3842, 4101, 4417, 4424
AfeI	AGCGCT	1 cut(s)	1682
AfiI	CCNNNNNNNGG	7 cut(s)	203, 1441, 1765, 2677, 2983, 3938, 4381
AflII	CTTAAG	2 cut(s)	1073, 2741
AhlI	ACTAGT	1 cut(s)	3545
AjiI	CACGTC	1 cut(s)	1477
AjuI	GAANNNNNNNTTGG	4 cut(s)	812, 844, 1814, 1846
Alw21I	GWGCWC	1 cut(s)	2740
Alw26I	GTCTC	6 cut(s)	503, 741, 1270, 1859, 2509, 3584
AlwI	GGATC	5 cut(s)	752, 1172, 1569, 3997, 4285
AlwNI	CAGNNNCTG	1 cut(s)	2032
Aor51HI	AGCGCT	1 cut(s)	1682
AoxI	GGCC	8 cut(s)	78, 91, 550, 1840, 3039, 3479, 3615, 4017
ApoI	RAATTY	8 cut(s)	14, 259, 2105, 2448, 2555, 3169, 3257, 3888
AscI	GGCGCGCC	1 cut(s)	384
AseI	ATTAAT	3 cut(s)	324, 1260, 1365
Asp718I	GGTACC	1 cut(s)	531
AspLEI	GCGC	9 cut(s)	386, 388, 1683, 2037, 2075, 3663, 3763, 4662, 4664
AspS9I	GGNCC	5 cut(s)	1306, 1741, 3273, 4018, 4384
AsuC2I	CCSGG	2 cut(s)	554, 4382
AsuHPI	GGTGA	2 cut(s)	497, 4220
AsuII	TTCGAA	2 cut(s)	2103, 3531
AvaII	GGWCC	4 cut(s)	1306, 1741, 3273, 4384
AxyI	CCTNAGG	1 cut(s)	2237
BalI	TGGCCA	1 cut(s)	93
BanI	GGYRCC	3 cut(s)	531, 3694, 3955
BanII	GRGCYC	1 cut(s)	1025
BauI	CACGAG	2 cut(s)	738, 4628
BbsI	GAAGAC	4 cut(s)	72, 1471, 2404, 4163
Bbv12I	GWGCWC	1 cut(s)	2740
BccI	CCATC	9 cut(s)	83, 145, 350, 1077, 1199, 1480, 1727, 2245, 4576
BceAI	ACGGC	4 cut(s)	1985, 2426, 2434, 3839
BcgI	CGANNNNNNTGC	4 cut(s)	2150, 2184, 4388, 4422
BclI	TGATCA	2 cut(s)	447, 1143
BcnI	CCSGG	2 cut(s)	554, 4382
BcoDI	GTCTC	6 cut(s)	503, 741, 1270, 1859, 2509, 3584
BcuI	ACTAGT	1 cut(s)	3545
BfmI	CTRYAG	3 cut(s)	1947, 2292, 2795
BfoI	RGCGCY	2 cut(s)	1684, 3764
BfrI	CTTAAG	2 cut(s)	1073, 2741
BfuAI	ACCTGC	1 cut(s)	488
BglI	GCCNNNNNGGC	1 cut(s)	2403
BmcAI	AGTACT	2 cut(s)	2283, 2290
Bme18I	GGWCC	4 cut(s)	1306, 1741, 3273, 4384
BmgBI	CACGTC	1 cut(s)	1477
BmgT120I	GGNCC	5 cut(s)	1306, 1741, 3273, 4018, 4384
BmiI	GGNNCC	6 cut(s)	533, 1307, 1791, 2955, 3696, 3957
BmrI	ACTGGG	1 cut(s)	4435
BmuI	ACTGGG	1 cut(s)	4435
BpiI	GAAGAC	4 cut(s)	72, 1471, 2404, 4163
BpmI	CTGGAG	6 cut(s)	225, 267, 1755, 3146, 3641, 4259
Bpu10I	CCTNAGC	1 cut(s)	951
Bpu14I	TTCGAA	2 cut(s)	2103, 3531
BpuEI	CTTGAG	2 cut(s)	2627, 4280
BpuMI	CCSGG	2 cut(s)	554, 4382
Bsa29I	ATCGAT	1 cut(s)	2381
BsaAI	YACGTR	1 cut(s)	2465
BsaBI	GATNNNNATC	1 cut(s)	3715
BsaHI	GRCGYC	2 cut(s)	3248, 3706
BsaI	GGTCTC	3 cut(s)	503, 741, 3584
BsaJI	CCNNGG	9 cut(s)	1017, 2416, 3317, 3460, 3594, 3754, 3969, 4063, 4458
BsaXI	ACNNNNNCTCC	2 cut(s)	4232, 4262
Bsc4I	CCNNNNNNNGG	7 cut(s)	203, 1441, 1765, 2677, 2983, 3938, 4381
Bse21I	CCTNAGG	1 cut(s)	2237
Bse3DI	GCAATG	5 cut(s)	145, 450, 2544, 2867, 3447
Bse8I	GATNNNNATC	1 cut(s)	3715
BseCI	ATCGAT	1 cut(s)	2381
BseDI	CCNNGG	9 cut(s)	1017, 2416, 3317, 3460, 3594, 3754, 3969, 4063, 4458
BseGI	GGATG	9 cut(s)	3328, 3744, 3903, 3948, 3965, 4294, 4435, 4468, 4540
BseJI	GATNNNNATC	1 cut(s)	3715
BseLI	CCNNNNNNNGG	7 cut(s)	203, 1441, 1765, 2677, 2983, 3938, 4381
BseMI	GCAATG	5 cut(s)	145, 450, 2544, 2867, 3447
BsePI	GCGCGC	2 cut(s)	384, 4660
BseRI	GAGGAG	2 cut(s)	1877, 2946
BseYI	CCCAGC	2 cut(s)	3844, 4223
BsgI	GTGCAG	3 cut(s)	783, 1209, 2540
Bsh1236I	CGCG	5 cut(s)	386, 1203, 3997, 4660, 4662
BshFI	GGCC	8 cut(s)	80, 93, 552, 1842, 3041, 3481, 3617, 4019
BshNI	GGYRCC	3 cut(s)	531, 3694, 3955
BshVI	ATCGAT	1 cut(s)	2381
BsiHKAI	GWGCWC	1 cut(s)	2740
BsiSI	CCGG	2 cut(s)	553, 4382
BslI	CCNNNNNNNGG	7 cut(s)	203, 1441, 1765, 2677, 2983, 3938, 4381
BsmAI	GTCTC	6 cut(s)	503, 741, 1270, 1859, 2509, 3584
BsmI	GAATGC	3 cut(s)	3586, 4193, 4548
BsnI	GGCC	8 cut(s)	80, 93, 552, 1842, 3041, 3481, 3617, 4019
Bso31I	GGTCTC	3 cut(s)	503, 741, 3584
Bsp119I	TTCGAA	2 cut(s)	2103, 3531
Bsp1286I	GDGCHC	2 cut(s)	1025, 2740
Bsp68I	TCGCGA	1 cut(s)	3997
BspANI	GGCC	8 cut(s)	80, 93, 552, 1842, 3041, 3481, 3617, 4019
BspDI	ATCGAT	1 cut(s)	2381
BspFNI	CGCG	5 cut(s)	386, 1203, 3997, 4660, 4662
BspHI	TCATGA	2 cut(s)	901, 1140
BspLI	GGNNCC	6 cut(s)	533, 1307, 1791, 2955, 3696, 3957
BspMAI	CTGCAG	1 cut(s)	2296
BspMI	ACCTGC	1 cut(s)	488
BspPI	GGATC	5 cut(s)	752, 1172, 1569, 3997, 4285
BspT104I	TTCGAA	2 cut(s)	2103, 3531
BspT107I	GGYRCC	3 cut(s)	531, 3694, 3955
BspTI	CTTAAG	2 cut(s)	1073, 2741
BspTNI	GGTCTC	3 cut(s)	503, 741, 3584
BsrBI	CCGCTC	2 cut(s)	3541, 4621
BsrDI	GCAATG	5 cut(s)	145, 450, 2544, 2867, 3447
BssECI	CCNNGG	9 cut(s)	1017, 2416, 3317, 3460, 3594, 3754, 3969, 4063, 4458
BssHII	GCGCGC	2 cut(s)	384, 4660
BssNAI	GTATAC	1 cut(s)	4394
BssNI	GRCGYC	2 cut(s)	3248, 3706
BssSI	CACGAG	2 cut(s)	738, 4628
BssT1I	CCWWGG	1 cut(s)	1017
Bst1107I	GTATAC	1 cut(s)	4394
Bst2BI	CACGAG	2 cut(s)	738, 4628
Bst6I	CTCTTC	2 cut(s)	2943, 3180
BstACI	GRCGYC	2 cut(s)	3248, 3706
BstAFI	CTTAAG	2 cut(s)	1073, 2741
BstAPI	GCANNNNNTGC	4 cut(s)	2032, 2756, 2878, 3680
BstBAI	YACGTR	1 cut(s)	2465
BstBI	TTCGAA	2 cut(s)	2103, 3531
BstDSI	CCRYGG	2 cut(s)	2416, 3754
BstF5I	GGATG	9 cut(s)	3328, 3744, 3903, 3948, 3965, 4294, 4435, 4468, 4540
BstFNI	CGCG	5 cut(s)	386, 1203, 3997, 4660, 4662
BstH2I	RGCGCY	2 cut(s)	1684, 3764
BstHHI	GCGC	9 cut(s)	386, 388, 1683, 2037, 2075, 3663, 3763, 4662, 4664
BstMAI	GTCTC	6 cut(s)	503, 741, 1270, 1859, 2509, 3584
BstNSI	RCATGY	2 cut(s)	1618, 2175
BstSFI	CTRYAG	3 cut(s)	1947, 2292, 2795
BstSNI	TACGTA	1 cut(s)	2465
BstUI	CGCG	5 cut(s)	386, 1203, 3997, 4660, 4662
BstV2I	GAAGAC	4 cut(s)	72, 1471, 2404, 4163
BstX2I	RGATCY	1 cut(s)	1561
BstYI	RGATCY	1 cut(s)	1561
BstZ17I	GTATAC	1 cut(s)	4394
Bsu15I	ATCGAT	1 cut(s)	2381
Bsu36I	CCTNAGG	1 cut(s)	2237
BsuRI	GGCC	8 cut(s)	80, 93, 552, 1842, 3041, 3481, 3617, 4019
BsuTUI	ATCGAT	1 cut(s)	2381
BtgI	CCRYGG	2 cut(s)	2416, 3754
BtgZI	GCGATG	2 cut(s)	1218, 1705
BtrI	CACGTC	1 cut(s)	1477
BtsCI	GGATG	9 cut(s)	3328, 3744, 3903, 3948, 3965, 4294, 4435, 4468, 4540
BtsI	GCAGTG	3 cut(s)	775, 2351, 3524
BtuMI	TCGCGA	1 cut(s)	3997
BveI	ACCTGC	1 cut(s)	488
CaiI	CAGNNNCTG	1 cut(s)	2032
CciI	TCATGA	2 cut(s)	901, 1140
CfoI	GCGC	9 cut(s)	386, 388, 1683, 2037, 2075, 3663, 3763, 4662, 4664
Cfr13I	GGNCC	5 cut(s)	1306, 1741, 3273, 4018, 4384
ClaI	ATCGAT	1 cut(s)	2381
CseI	GACGC	4 cut(s)	2273, 3129, 3256, 4360
Csp6I	GTAC	9 cut(s)	532, 677, 2282, 2289, 2318, 3841, 4100, 4416, 4423
CviQI	GTAC	9 cut(s)	532, 677, 2282, 2289, 2318, 3841, 4100, 4416, 4423
DraI	TTTAAA	4 cut(s)	264, 2566, 3036, 4086
DraIII	CACNNNGTG	2 cut(s)	1441, 3770
EaeI	YGGCCR	2 cut(s)	91, 550
Eam1104I	CTCTTC	2 cut(s)	2943, 3180
EarI	CTCTTC	2 cut(s)	2943, 3180
EciI	GGCGGA	2 cut(s)	1599, 3822
Eco105I	TACGTA	1 cut(s)	2465
Eco130I	CCWWGG	1 cut(s)	1017
Eco147I	AGGCCT	3 cut(s)	1842, 3041, 3481
Eco24I	GRGCYC	1 cut(s)	1025
Eco31I	GGTCTC	3 cut(s)	503, 741, 3584
Eco32I	GATATC	3 cut(s)	667, 1315, 3610
Eco47I	GGWCC	4 cut(s)	1306, 1741, 3273, 4384
Eco47III	AGCGCT	1 cut(s)	1682
Eco57I	CTGAAG	3 cut(s)	585, 1068, 1173
Eco81I	CCTNAGG	1 cut(s)	2237
EcoO109I	RGGNCCY	1 cut(s)	1306
EcoRI	GAATTC	2 cut(s)	2105, 2555
EcoRV	GATATC	3 cut(s)	667, 1315, 3610
EcoT14I	CCWWGG	1 cut(s)	1017
EcoT22I	ATGCAT	4 cut(s)	1114, 1126, 3348, 3449
EcoT38I	GRGCYC	1 cut(s)	1025
ErhI	CCWWGG	1 cut(s)	1017
FalI	AAGNNNNNCTT	2 cut(s)	3177, 3209
FauNDI	CATATG	1 cut(s)	3877
FbaI	TGATCA	2 cut(s)	447, 1143
FblI	GTMKAC	1 cut(s)	4393
FokI	GGATG	9 cut(s)	3335, 3751, 3910, 3935, 3952, 4301, 4442, 4475, 4547
FriOI	GRGCYC	1 cut(s)	1025
GlaI	GCGC	9 cut(s)	385, 387, 1682, 2036, 2074, 3662, 3762, 4661, 4663
GsaI	CCCAGC	2 cut(s)	3848, 4227
GsuI	CTGGAG	6 cut(s)	225, 267, 1755, 3146, 3641, 4259
HaeII	RGCGCY	2 cut(s)	1684, 3764
HaeIII	GGCC	8 cut(s)	80, 93, 552, 1842, 3041, 3481, 3617, 4019
HapII	CCGG	2 cut(s)	553, 4382
HgaI	GACGC	4 cut(s)	2273, 3129, 3256, 4360
HhaI	GCGC	9 cut(s)	386, 388, 1683, 2037, 2075, 3663, 3763, 4662, 4664
Hin1I	GRCGYC	2 cut(s)	3248, 3706
Hin6I	GCGC	9 cut(s)	384, 386, 1681, 2035, 2073, 3661, 3761, 4660, 4662
HinP1I	GCGC	9 cut(s)	384, 386, 1681, 2035, 2073, 3661, 3761, 4660, 4662
HincII	GTYRAC	4 cut(s)	1629, 2017, 3028, 4564
HindII	GTYRAC	4 cut(s)	1629, 2017, 3028, 4564
HindIII	AAGCTT	6 cut(s)	1608, 1652, 1910, 3191, 3976, 4026
HpaII	CCGG	2 cut(s)	553, 4382
HphI	GGTGA	2 cut(s)	497, 4220
Hpy166II	GTNNAC	9 cut(s)	1629, 2017, 2318, 2468, 3028, 3814, 4128, 4394, 4564
Hpy8I	GTNNAC	9 cut(s)	1629, 2017, 2318, 2468, 3028, 3814, 4128, 4394, 4564
Hpy99I	CGWCG	5 cut(s)	2162, 3123, 3708, 3711, 3997
HpyCH4IV	ACGT	9 cut(s)	429, 868, 1476, 1554, 2013, 2464, 2548, 2809, 3706
HpySE526I	ACGT	9 cut(s)	429, 868, 1476, 1554, 2013, 2464, 2548, 2809, 3706
Hsp92I	GRCGYC	2 cut(s)	3248, 3706
HspAI	GCGC	9 cut(s)	384, 386, 1681, 2035, 2073, 3661, 3761, 4660, 4662
KpnI	GGTACC	1 cut(s)	535
Ksp22I	TGATCA	2 cut(s)	447, 1143
MaeII	ACGT	9 cut(s)	429, 868, 1476, 1554, 2013, 2464, 2548, 2809, 3706
MaeIII	GTNAC	3 cut(s)	359, 864, 1100
MbiI	CCGCTC	2 cut(s)	3541, 4621
MfeI	CAATTG	1 cut(s)	1269
MflI	RGATCY	1 cut(s)	1561
MhlI	GDGCHC	2 cut(s)	1025, 2740
MlsI	TGGCCA	1 cut(s)	93
MluNI	TGGCCA	1 cut(s)	93
MlyI	GAGTC	5 cut(s)	62, 2441, 3059, 3380, 3809
MmeI	TCCRAC	6 cut(s)	919, 2781, 3354, 3654, 4527, 4626
Mox20I	TGGCCA	1 cut(s)	93
Mph1103I	ATGCAT	4 cut(s)	1114, 1126, 3348, 3449
MscI	TGGCCA	1 cut(s)	93
MslI	CAYNNNNRTG	2 cut(s)	518, 2372
Msp20I	TGGCCA	1 cut(s)	93
MspA1I	CMGCKG	3 cut(s)	2311, 2438, 3299
MspCI	CTTAAG	2 cut(s)	1073, 2741
MspI	CCGG	2 cut(s)	553, 4382
MunI	CAATTG	1 cut(s)	1269
Mva1269I	GAATGC	3 cut(s)	3586, 4193, 4548
MvnI	CGCG	5 cut(s)	386, 1203, 3997, 4660, 4662
NciI	CCSGG	2 cut(s)	554, 4382
NdeI	CATATG	1 cut(s)	3877
NlaIV	GGNNCC	6 cut(s)	533, 1307, 1791, 2955, 3696, 3957
NmuCI	GTSAC	3 cut(s)	359, 864, 1100
NruI	TCGCGA	1 cut(s)	3997
NsiI	ATGCAT	4 cut(s)	1114, 1126, 3348, 3449
NspI	RCATGY	2 cut(s)	1618, 2175
NspV	TTCGAA	2 cut(s)	2103, 3531
PaeI	GCATGC	1 cut(s)	2175
PagI	TCATGA	2 cut(s)	901, 1140
PalAI	GGCGCGCC	1 cut(s)	384
PauI	GCGCGC	2 cut(s)	384, 4660
PceI	AGGCCT	3 cut(s)	1842, 3041, 3481
PctI	GAATGC	3 cut(s)	3586, 4193, 4548
PflMI	CCANNNNNTGG	3 cut(s)	1441, 1765, 2983
PfoI	TCCNGGA	1 cut(s)	3162
PleI	GAGTC	5 cut(s)	62, 2440, 3058, 3379, 3809
PpsI	GAGTC	5 cut(s)	62, 2440, 3058, 3379, 3809
Ppu21I	YACGTR	1 cut(s)	2465
PpuMI	RGGWCCY	1 cut(s)	1306
PshBI	ATTAAT	3 cut(s)	324, 1260, 1365
PsiI	TTATAA	1 cut(s)	1688
Psp1406I	AACGTT	1 cut(s)	2809
Psp5II	RGGWCCY	1 cut(s)	1306
PspFI	CCCAGC	2 cut(s)	3844, 4223
PspN4I	GGNNCC	6 cut(s)	533, 1307, 1791, 2955, 3696, 3957
PspPI	GGNCC	5 cut(s)	1306, 1741, 3273, 4018, 4384
PspPPI	RGGWCCY	1 cut(s)	1306
PstI	CTGCAG	1 cut(s)	2296
PstNI	CAGNNNCTG	1 cut(s)	2032
PsuI	RGATCY	1 cut(s)	1561
PteI	GCGCGC	2 cut(s)	384, 4660
PvuII	CAGCTG	2 cut(s)	2438, 3299
RruI	TCGCGA	1 cut(s)	3997
RsaI	GTAC	9 cut(s)	533, 678, 2283, 2290, 2319, 3842, 4101, 4417, 4424
RsaNI	GTAC	9 cut(s)	532, 677, 2282, 2289, 2318, 3841, 4100, 4416, 4423
RseI	CAYNNNNRTG	2 cut(s)	518, 2372
Sau96I	GGNCC	5 cut(s)	1306, 1741, 3273, 4018, 4384
ScaI	AGTACT	2 cut(s)	2283, 2290
SchI	GAGTC	5 cut(s)	62, 2441, 3059, 3380, 3809
SduI	GDGCHC	2 cut(s)	1025, 2740
SfcI	CTRYAG	3 cut(s)	1947, 2292, 2795
SfuI	TTCGAA	2 cut(s)	2103, 3531
SgsI	GGCGCGCC	1 cut(s)	384
SinI	GGWCC	4 cut(s)	1306, 1741, 3273, 4384
SmiMI	CAYNNNNRTG	2 cut(s)	518, 2372
SmlI	CTYRAG	4 cut(s)	1073, 2642, 2741, 4295
SmoI	CTYRAG	4 cut(s)	1073, 2642, 2741, 4295
SnaBI	TACGTA	1 cut(s)	2465
SpeI	ACTAGT	1 cut(s)	3545
SphI	GCATGC	1 cut(s)	2175
SseBI	AGGCCT	3 cut(s)	1842, 3041, 3481
SspI	AATATT	3 cut(s)	1006, 3778, 4137
StuI	AGGCCT	3 cut(s)	1842, 3041, 3481
StyI	CCWWGG	1 cut(s)	1017
TaiI	ACGT	9 cut(s)	432, 871, 1479, 1557, 2016, 2467, 2551, 2812, 3709
TatI	WGTACW	5 cut(s)	676, 2281, 2288, 4415, 4422
TauI	GCSGC	4 cut(s)	346, 1203, 2314, 4660
TseFI	GTSAC	3 cut(s)	359, 864, 1100
Tsp45I	GTSAC	3 cut(s)	359, 864, 1100
TspGWI	ACGGA	6 cut(s)	586, 781, 1724, 2051, 3743, 4549
Van91I	CCANNNNNTGG	3 cut(s)	1441, 1765, 2983
Vha464I	CTTAAG	2 cut(s)	1073, 2741
VpaK11BI	GGWCC	4 cut(s)	1306, 1741, 3273, 4384
VspI	ATTAAT	3 cut(s)	324, 1260, 1365
XapI	RAATTY	8 cut(s)	14, 259, 2105, 2448, 2555, 3169, 3257, 3888
XceI	RCATGY	2 cut(s)	1618, 2175
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3976
XmiI	GTMKAC	1 cut(s)	4393
ZraI	GACGTC	1 cut(s)	3707
ZrmI	AGTACT	2 cut(s)	2283, 2290
Zsp2I	ATGCAT	4 cut(s)	1114, 1126, 3348, 3449

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.