Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0005571

Physical Location & Seq

Genomic Coordinates Reverse (-)

23325444 .. 23333823

Gene Structure 8380 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom12g22440.19

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3936 bp

ATGGGAACACCAGTGAATATCATTGTAGGTTCTCAAGTCTGGGTTGAAGACTCAGAATTGGCCTGGATTGATGGCCAAGTGTCAAAGATTACAGGACAAGAAGCTGAGATCGAGAGTAGCAATGGGAAGAAGATGGTTGTCAAATTGGCGAAAATATATCCAAAAGATATGGAAGCTCCTGCTGCTGGAGTTGATGACATGACAAAGCTATCTTATCTGCATGAGCCTGGAGTTTTGCAGAATTTAAAAACTAGATACGAACTTAATGAAATCTATACTTATACTGGAAATATCCTTATTGCCATTAATCCATTCCAAAGACTGCCGCATCTCTACGATGGTCACATGATGCAACAATACAAGGGCGCGCCATTTGGAGAACTAAATCCTCATGTTTTTGCAGTTGCGGATGTTGCATATAGGGCGATGATCAATGAGGGAAAAAGCAATTCAATTCTGGTCAGTGGTGAAAGTGGAGCAGGTAAAACTGAGACCACAAAAATGCTTATGAGGTACCTTGCGTTTCTAGGTGGCCGGGCTGCAACTGAAGGACGGACAGTTGAACAGCAAGTTCTTGAATCAAATCCAGTTCTGGAAGCATTTGGCAACGCTAAAACTGTTAGAAATAACAATTCTAGTCGTTTTGGTAAATTTGTTGAAATTCAATTTGATAAGCAAGGAAGAATCTCAGGAGCAGCCATTAGAACCTACCTTCTGGAACGGTCTCGTGTTTGCCAAGTTTCTGATCCTGAACGGAACTACCACTGCTTCTACCTTCTTTGTGCTGCACCACAGGAGGAAATTGAGAAATACAAGTTGGCAAATCCTAAATCATTTCACTATCTTAACCAGTCACGTTGCTATGAACTGGTTGGTGTAAGTGATGCTCATGATTATCTTGCCACAAGGAGAGCTATGGATATTGTTGGAATAAGTGCTAAGGAGCAGGAAGCTATTTTTAGAGTAGTTGCTGCAATTCTTCACCTCGGAAATATTGAGTTTTCCAAGGGCTCGGAAGTTGATTCGTCAGTTCCAAAAGATGATAAAGCAAAGTTCCATCTTAAGATGACTTCAGAGCTTCTCATGTGTGACCCTGATGCATTGGAGGATGCATTATGTAAGCGTGTCATGATCACACCTGAAGAAGTTATCAAGAGAAGTCTTGATCCTCAAAGTGCAGCAATTAGCCGCGATGGTTTAGCAAAGACAGTATATTCTCGATTGTTTGATTGGTTGGTGGACAAAATTAACGTCTCAATTGGACAAGATGCTACCTCGAAATCGCTGATTGGGGTCCTTGATATCTATGGTTTTGAAAGTTTTAAGACCAATAGTTTTGAGCAGTTCTGCATTAATTTCACAAACGAGAAATTGCAGCAACATTTCAATCAGCACGTCTTCAAGATGGAACAAGAAGAATACACAAAAGAGCAGATTGATTGGAGCTACATTGAGTTTGTCGATAATCAAGACGTTTTGGATCTTATTGAAAAGAAACCTGGCGGAATAGTTGCTCTCCTTGATGAAGCTTGCATGTTTCCCAAGTCAACCCATGAAACATTTGCAAATAAGCTTTATCAGACATTCAAAACTCACAAGCGCTTTATAAAGCCAAAACTTTCTCGCACGGATTTTACCATTGCTCATTATGCTGGAGAGGTCCAATATCAGTCTGACCAATTTTTGGACAAAAACAAGGACTACGTGGTTCCTGAACATCAAGATTTGTTGGGTGTTTCCAAATGTCCTTTCGTGGCAGGCCTTTTCCCTCCACTTCCAGAGGAGACAGCCAAATCTTCCAAGTTTTCTTCGATTGGCTCTCGTTTTAAGCTTCAACTACAATCGTTAATGGAAACCCTAAACTCTACAGAACCTCACTATGTCAGATGTGTAAAGCCAAATAATTTTCTAAAACCAGCCGTGTTTGAGAACGTCAACATTATGCAGCAACTGCGCTGTGGTGGTGTTTTAGAGGCAATCCGTATTAGCTGCGCTGGCTACCCTACTCGAAAACCCTTTTTCGAATTCATAAACAGATTTGGACTTCTTGCACCAGAGGTCTTGGAAGGAAACTACGACGAAAAGGTTGCATGCACTAAGATTCTTGAAAAGAAGGGTCTTAAAGGGTTTCAGATAGGTAAAACAAAGGTATTCCTGAGGGCTGGTCAGATGGCTGAGTTAGATGCACGAAGAGCCGAAGTACTCAGTACTGCAGCTAAAACTATACAGCGGCGTGTACGAACCCATTATGCTCGGAAACGGTTTATTGCACTGCGACGGGCGACCATAGTTATGCAATCGATATGCAGAGGAAGCCTGGCTGGCAAAGTCTTCCACGGCATGAAAAGAGAGTCAGCTGCCGTGAAAATTCAGAAGAACCTACGTAAACACCAAGCCAGAACAACTTACAACAAGCTCCATGTCTCTGTTCTTGTCGTGCAGACAGGTTTAAGAGCAATGGCTGGACGTAAACAGTTCAGATTTAAAAGGCAAACCAAAGCAGCAACCATTGTTCAGGCGGTGTGGCGTTGTCATAAAGCTGCCAAATACTTTAAGCGGCTCAAGAGGGGTTCAGTAGTTGCACAGTGCCGAATGAGGGGGAAAATCGCAAGGAAGGAACTTAGGAAGCTCAAAATGGCTGCAAGGGAAACAGGAGCACTTAAGGAAGCAAAAGATGCACTGGAAAAACGAGTGGAGGAACTAAATTGGCGACTACAGTTGGAAAAACGTTTAAGGACTGACCTAGAAGAAGCAAAAGCACAAGAGACAACGAAATTGCAAAACTCATTGCAAGAAATGCAGACCAAACTTGATGAATCAAACGCATTGATTGTGAAGGAACGAGAGGCTGCAAAGAAAGCTATTGAAGAGGCACCTCCTGTGGTTCAGGAAACCCAAGTTATGGTTGAGGACACTGAAAAAGTTGAATCTCTAACTGCAGAAGTTGACAGTTTAAAGGCCTCTATGGAGTCAGAGAAACAAAGAGCAGAAGAAAATGAAAGAAAATATAATGAAGCTCAAACATCGGGCGAAGAACGACGCAAAAAATTAGAAGAAACAGAGAAAAAAGTTAGTCAGCTCCAGGAAAATTTGACCAGGCTAGAAGAGAAGCTTGCCAATTTAGAATCAGAAAATCAAGTATTACGCCAACAAGCTGTGTCCATGACGCCTAATAAATTCCTTTCTGGACGGTCCAGATCAACTATTCAGAGGGCAGCTGAAAGTGGTCATATTCCAGGGGATGCAAAGACAGCTCTGGAAATGCATAGTCCTTCAATAAATCACAGGGAGTCGGAATTGGAGGATAAGCCTCAGAAATCACTAAATGAAAAACAGCAAGAGAACCAGGAGTTGCTCATTCGATGCATCGCACAACACCTGGGCTTTGCAGGGAACAGGCCTATTGCTGCCTGCATCATATACAAATGCCTTTTGCAGTGGAGATCATTTGAAGTTGAGCGGACTAGCATTTTCGATAGGATTATTCAGACCATAGGGAATGCCATTGAGACCCAGGATAACAACGATATCTTGGCCTATTGGCTATCCAATGCCTCAACACTTCTATTACTACTCCAGCGCACACTGAAAGCAAGCGGTGCTGCTGGAATGGCACCCCAGCGACGTCGATCATCATCAGCTACTCTGTTTGGAAGGATGACACAAAGTTTCCGTGGAGCCCCACAAGGTGTTAATATTTCCTTAATCAATGGTGGAATGAGTGGCGGAGTGGACTCTTTACGGCAAGTTGAAGCCAAGTACCCAGCTTTGCTTTTCAAACAGCAACTTACAGCATATGTAGAAAAGATTTATGGGATGATTCGAGATAATTTGAAGAAAGAGATTTCTCCTTTGCTTGGATTGTGCATCCAGGCACCAAGAACTTCCAGGGCAAGCTTGGTCAAAGGATCGATCACGATCAGTTGCCAACACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000146 GO:0000166 GO:0000331 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003774 GO:0003779 GO:0003824 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005856 GO:0006928 GO:0006996 GO:0007010 GO:0007015 GO:0008092 GO:0008144 GO:0008150 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0015629 GO:0016043 GO:0016049 GO:0016459 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0022414 GO:0030029 GO:0030036 GO:0030048 GO:0030154 GO:0030554 GO:0030898 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032989 GO:0032991 GO:0033275 GO:0035639 GO:0036094 GO:0040007 GO:0042623 GO:0042641 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043531 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044706 GO:0044877 GO:0048468 GO:0048588 GO:0048589 GO:0048856 GO:0048868 GO:0048869 GO:0051015 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051656 GO:0051704 GO:0060560 GO:0070252 GO:0071840 GO:0097159 GO:0097367 GO:0097435 GO:0097708 GO:0140027 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DIL IQ Myosin_N Myosin_head

Protein Analysis

1312

Amino Acids

148.23

Weight (kDa)

9.23

Isoelectric Point (pI)

48.57

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005571)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G08730 AT1G08730 AT1G54560 AT1G54560
fragaria_vesca	FvH4_6g02450 FvH4_6g02450
malus_domestica	MD04G1227800.v1.1 MD12G1244600.v1.1
prunus_persica	Prupe.6G347100_v2.0.a1 Prupe.6G347100_v2.0.a1 Prupe.6G347100_v2.0.a1 Prupe.6G347100_v2.0.a1
pyrus_communis	pycom04g20130 pycom04g20140 pycom04g20150 pycom12g22440
rosa_chinensis	RchiOBHm_Chr3g0450241 RchiOBHm_Chr3g0450271
rosa_laevigata	RLG00000025743 RLG00000025747
rosa_multiflora	Rmu_sc0010184.1_g000002
rosa_roxburghii	Rroxscaffold_6G00427950
rosa_rugosa	Rorug02G0626300 Rorug02G0626400
rosa_samantha	Rh3AG025600 Rh3BG026300 Rh3CG025300
rosa_wichuraiana	Rw3G002080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1607
AatII	GACGTC	1 cut(s)	3628
Acc36I	ACCTGC	1 cut(s)	470
Acc65I	GGTACC	1 cut(s)	513
AccB1I	GGYRCC	4 cut(s)	513, 2872, 3613, 3874
AccB7I	CCANNNNNTGG	2 cut(s)	1684, 2902
AccBSI	CCGCTC	1 cut(s)	3460
AccII	CGCG	2 cut(s)	368, 1191
AclI	AACGTT	1 cut(s)	2728
AclWI	GGATC	4 cut(s)	740, 1160, 1488, 3916
AcoI	YGGCCR	2 cut(s)	73, 532
AcsI	RAATTY	7 cut(s)	241, 650, 660, 2024, 2367, 3088, 3176
AcuI	CTGAAG	3 cut(s)	567, 1056, 1161
AcyI	GRCGYC	2 cut(s)	3167, 3625
AdeI	CACNNNGTG	1 cut(s)	3689
AfaI	GTAC	5 cut(s)	515, 2202, 2209, 2238, 3761
AfeI	AGCGCT	1 cut(s)	1601
AfiI	CCNNNNNNNGG	5 cut(s)	185, 1684, 2596, 2902, 3857
AflII	CTTAAG	2 cut(s)	1061, 2660
AjiI	CACGTC	1 cut(s)	1396
AjuI	GAANNNNNNNTTGG	4 cut(s)	800, 832, 1733, 1765
Alw21I	GWGCWC	1 cut(s)	2659
Alw26I	GTCTC	7 cut(s)	485, 729, 1258, 1778, 2428, 2759, 3503
AlwI	GGATC	4 cut(s)	740, 1160, 1488, 3916
AlwNI	CAGNNNCTG	1 cut(s)	1951
Aor51HI	AGCGCT	1 cut(s)	1601
AoxI	GGCC	7 cut(s)	60, 73, 532, 1759, 2958, 3398, 3534
ApoI	RAATTY	7 cut(s)	241, 650, 660, 2024, 2367, 3088, 3176
ArsI	GACNNNNNNTTYG	2 cut(s)	1236, 1268
AscI	GGCGCGCC	1 cut(s)	366
AseI	ATTAAT	2 cut(s)	306, 1353
Asp718I	GGTACC	1 cut(s)	513
AspLEI	GCGC	6 cut(s)	368, 370, 1602, 1956, 1994, 3582
AspS9I	GGNCC	3 cut(s)	1294, 1660, 3192
AsuC2I	CCSGG	1 cut(s)	536
AsuHPI	GGTGA	2 cut(s)	479, 974
AsuII	TTCGAA	1 cut(s)	2022
AvaII	GGWCC	3 cut(s)	1294, 1660, 3192
AxyI	CCTNAGG	1 cut(s)	2156
BalI	TGGCCA	1 cut(s)	75
BanI	GGYRCC	4 cut(s)	513, 2872, 3613, 3874
BanII	GRGCYC	2 cut(s)	1013, 3682
BauI	CACGAG	1 cut(s)	726
BbsI	GAAGAC	3 cut(s)	54, 1390, 2323
Bbv12I	GWGCWC	1 cut(s)	2659
BccI	CCATC	7 cut(s)	65, 127, 332, 1065, 1187, 1399, 2164
BceAI	ACGGC	4 cut(s)	1904, 2345, 2353, 3758
BcgI	CGANNNNNNTGC	2 cut(s)	2069, 2103
BclI	TGATCA	2 cut(s)	429, 1131
BcnI	CCSGG	1 cut(s)	536
BcoDI	GTCTC	7 cut(s)	485, 729, 1258, 1778, 2428, 2759, 3503
BfaI	CTAG	6 cut(s)	252, 527, 636, 2744, 3101, 3465
BfmI	CTRYAG	4 cut(s)	1866, 2211, 2714, 2937
BfoI	RGCGCY	1 cut(s)	1603
BfrI	CTTAAG	2 cut(s)	1061, 2660
BfuAI	ACCTGC	1 cut(s)	470
BglI	GCCNNNNNGGC	1 cut(s)	2322
BmcAI	AGTACT	2 cut(s)	2202, 2209
Bme18I	GGWCC	3 cut(s)	1294, 1660, 3192
BmgBI	CACGTC	1 cut(s)	1396
BmgT120I	GGNCC	3 cut(s)	1294, 1660, 3192
BmiI	GGNNCC	7 cut(s)	515, 1295, 1710, 2874, 3615, 3679, 3876
BpiI	GAAGAC	3 cut(s)	54, 1390, 2323
BpmI	CTGGAG	5 cut(s)	207, 249, 1674, 3065, 3560
Bpu10I	CCTNAGC	1 cut(s)	939
Bpu14I	TTCGAA	1 cut(s)	2022
BpuEI	CTTGAG	2 cut(s)	18, 2546
BpuMI	CCSGG	1 cut(s)	536
Bsa29I	ATCGAT	2 cut(s)	2300, 3911
BsaAI	YACGTR	2 cut(s)	1705, 2384
BsaBI	GATNNNNATC	2 cut(s)	3634, 3917
BsaHI	GRCGYC	2 cut(s)	3167, 3625
BsaI	GGTCTC	3 cut(s)	485, 729, 3503
BsaJI	CCNNGG	8 cut(s)	985, 1005, 2335, 3236, 3379, 3513, 3673, 3888
Bsc4I	CCNNNNNNNGG	5 cut(s)	185, 1684, 2596, 2902, 3857
Bse1I	ACTGG	6 cut(s)	11, 289, 587, 850, 873, 2685
Bse21I	CCTNAGG	1 cut(s)	2156
Bse3DI	GCAATG	4 cut(s)	127, 1638, 2463, 2786
Bse8I	GATNNNNATC	2 cut(s)	3634, 3917
BseCI	ATCGAT	2 cut(s)	2300, 3911
BseDI	CCNNGG	8 cut(s)	985, 1005, 2335, 3236, 3379, 3513, 3673, 3888
BseGI	GGATG	6 cut(s)	415, 1114, 3247, 3663, 3822, 3867
BseJI	GATNNNNATC	2 cut(s)	3634, 3917
BseLI	CCNNNNNNNGG	5 cut(s)	185, 1684, 2596, 2902, 3857
BseMI	GCAATG	4 cut(s)	127, 1638, 2463, 2786
BseMII	CTCAG	8 cut(s)	66, 96, 480, 702, 2147, 2166, 2218, 3326
BseNI	ACTGG	6 cut(s)	11, 289, 587, 850, 873, 2685
BsePI	GCGCGC	1 cut(s)	366
BseRI	GAGGAG	1 cut(s)	1796
BseYI	CCCAGC	2 cut(s)	3618, 3763
BsgI	GTGCAG	3 cut(s)	771, 1197, 2459
Bsh1236I	CGCG	2 cut(s)	368, 1191
BshFI	GGCC	7 cut(s)	62, 75, 534, 1761, 2960, 3400, 3536
BshNI	GGYRCC	4 cut(s)	513, 2872, 3613, 3874
BshVI	ATCGAT	2 cut(s)	2300, 3911
BsiHKAI	GWGCWC	1 cut(s)	2659
BsiSI	CCGG	1 cut(s)	535
BslI	CCNNNNNNNGG	5 cut(s)	185, 1684, 2596, 2902, 3857
BsmAI	GTCTC	7 cut(s)	485, 729, 1258, 1778, 2428, 2759, 3503
BsmBI	CGTCTC	1 cut(s)	1258
BsmI	GAATGC	1 cut(s)	3505
BsnI	GGCC	7 cut(s)	62, 75, 534, 1761, 2960, 3400, 3536
Bso31I	GGTCTC	3 cut(s)	485, 729, 3503
Bsp119I	TTCGAA	1 cut(s)	2022
Bsp1286I	GDGCHC	3 cut(s)	1013, 2659, 3682
BspANI	GGCC	7 cut(s)	62, 75, 534, 1761, 2960, 3400, 3536
BspCNI	CTCAG	8 cut(s)	65, 97, 481, 701, 2148, 2167, 2217, 3325
BspDI	ATCGAT	2 cut(s)	2300, 3911
BspFNI	CGCG	2 cut(s)	368, 1191
BspHI	TCATGA	2 cut(s)	889, 1128
BspLI	GGNNCC	7 cut(s)	515, 1295, 1710, 2874, 3615, 3679, 3876
BspMAI	CTGCAG	2 cut(s)	2215, 2941
BspMI	ACCTGC	1 cut(s)	470
BspPI	GGATC	4 cut(s)	740, 1160, 1488, 3916
BspQI	GCTCTTC	1 cut(s)	2185
BspT104I	TTCGAA	1 cut(s)	2022
BspT107I	GGYRCC	4 cut(s)	513, 2872, 3613, 3874
BspTI	CTTAAG	2 cut(s)	1061, 2660
BspTNI	GGTCTC	3 cut(s)	485, 729, 3503
BsrBI	CCGCTC	1 cut(s)	3460
BsrDI	GCAATG	4 cut(s)	127, 1638, 2463, 2786
BsrI	ACTGG	6 cut(s)	11, 289, 587, 850, 873, 2685
BssECI	CCNNGG	8 cut(s)	985, 1005, 2335, 3236, 3379, 3513, 3673, 3888
BssHII	GCGCGC	1 cut(s)	366
BssNI	GRCGYC	2 cut(s)	3167, 3625
BssSI	CACGAG	1 cut(s)	726
BssT1I	CCWWGG	1 cut(s)	1005
Bst2BI	CACGAG	1 cut(s)	726
Bst6I	CTCTTC	3 cut(s)	2185, 2862, 3099
BstACI	GRCGYC	2 cut(s)	3167, 3625
BstAFI	CTTAAG	2 cut(s)	1061, 2660
BstAPI	GCANNNNNTGC	4 cut(s)	1951, 2675, 2797, 3599
BstBAI	YACGTR	2 cut(s)	1705, 2384
BstBI	TTCGAA	1 cut(s)	2022
BstDSI	CCRYGG	2 cut(s)	2335, 3673
BstF5I	GGATG	6 cut(s)	415, 1114, 3247, 3663, 3822, 3867
BstFNI	CGCG	2 cut(s)	368, 1191
BstH2I	RGCGCY	1 cut(s)	1603
BstHHI	GCGC	6 cut(s)	368, 370, 1602, 1956, 1994, 3582
BstMAI	GTCTC	7 cut(s)	485, 729, 1258, 1778, 2428, 2759, 3503
BstNSI	RCATGY	2 cut(s)	1537, 2094
BstSFI	CTRYAG	4 cut(s)	1866, 2211, 2714, 2937
BstSNI	TACGTA	1 cut(s)	2384
BstUI	CGCG	2 cut(s)	368, 1191
BstV2I	GAAGAC	3 cut(s)	54, 1390, 2323
BstX2I	RGATCY	1 cut(s)	1480
BstYI	RGATCY	1 cut(s)	1480
Bsu15I	ATCGAT	2 cut(s)	2300, 3911
Bsu36I	CCTNAGG	1 cut(s)	2156
BsuRI	GGCC	7 cut(s)	62, 75, 534, 1761, 2960, 3400, 3536
BsuTUI	ATCGAT	2 cut(s)	2300, 3911
BtgI	CCRYGG	2 cut(s)	2335, 3673
BtgZI	GCGATG	3 cut(s)	440, 1206, 3352
BtrI	CACGTC	1 cut(s)	1396
BtsCI	GGATG	6 cut(s)	415, 1114, 3247, 3663, 3822, 3867
BtsI	GCAGTG	3 cut(s)	763, 2270, 3443
BveI	ACCTGC	1 cut(s)	470
CaiI	CAGNNNCTG	1 cut(s)	1951
CciI	TCATGA	2 cut(s)	889, 1128
CfoI	GCGC	6 cut(s)	368, 370, 1602, 1956, 1994, 3582
Cfr13I	GGNCC	3 cut(s)	1294, 1660, 3192
ClaI	ATCGAT	2 cut(s)	2300, 3911
CseI	GACGC	2 cut(s)	3048, 3175
Csp6I	GTAC	5 cut(s)	514, 2201, 2208, 2237, 3760
CviQI	GTAC	5 cut(s)	514, 2201, 2208, 2237, 3760
DraI	TTTAAA	3 cut(s)	246, 2485, 2955
DraIII	CACNNNGTG	1 cut(s)	3689
EaeI	YGGCCR	2 cut(s)	73, 532
Eam1104I	CTCTTC	3 cut(s)	2185, 2862, 3099
EarI	CTCTTC	3 cut(s)	2185, 2862, 3099
EciI	GGCGGA	2 cut(s)	1518, 3741
Eco105I	TACGTA	1 cut(s)	2384
Eco130I	CCWWGG	1 cut(s)	1005
Eco147I	AGGCCT	3 cut(s)	1761, 2960, 3400
Eco24I	GRGCYC	2 cut(s)	1013, 3682
Eco31I	GGTCTC	3 cut(s)	485, 729, 3503
Eco32I	GATATC	2 cut(s)	1303, 3529
Eco47I	GGWCC	3 cut(s)	1294, 1660, 3192
Eco47III	AGCGCT	1 cut(s)	1601
Eco57I	CTGAAG	3 cut(s)	567, 1056, 1161
Eco81I	CCTNAGG	1 cut(s)	2156
EcoO109I	RGGNCCY	1 cut(s)	1294
EcoRI	GAATTC	1 cut(s)	2024
EcoRV	GATATC	2 cut(s)	1303, 3529
EcoT14I	CCWWGG	1 cut(s)	1005
EcoT22I	ATGCAT	4 cut(s)	1102, 1114, 3267, 3368
EcoT38I	GRGCYC	2 cut(s)	1013, 3682
ErhI	CCWWGG	1 cut(s)	1005
Esp3I	CGTCTC	1 cut(s)	1258
FalI	AAGNNNNNCTT	2 cut(s)	3096, 3128
FauNDI	CATATG	1 cut(s)	3796
FbaI	TGATCA	2 cut(s)	429, 1131
FokI	GGATG	6 cut(s)	422, 1121, 3254, 3670, 3829, 3854
FriOI	GRGCYC	2 cut(s)	1013, 3682
FspBI	CTAG	6 cut(s)	252, 527, 636, 2744, 3101, 3465
GlaI	GCGC	6 cut(s)	367, 369, 1601, 1955, 1993, 3581
GsaI	CCCAGC	2 cut(s)	3622, 3767
GsuI	CTGGAG	5 cut(s)	207, 249, 1674, 3065, 3560
HaeII	RGCGCY	1 cut(s)	1603
HaeIII	GGCC	7 cut(s)	62, 75, 534, 1761, 2960, 3400, 3536
HapII	CCGG	1 cut(s)	535
HgaI	GACGC	2 cut(s)	3048, 3175
HhaI	GCGC	6 cut(s)	368, 370, 1602, 1956, 1994, 3582
Hin1I	GRCGYC	2 cut(s)	3167, 3625
Hin6I	GCGC	6 cut(s)	366, 368, 1600, 1954, 1992, 3580
HinP1I	GCGC	6 cut(s)	366, 368, 1600, 1954, 1992, 3580
HincII	GTYRAC	3 cut(s)	1548, 1936, 2947
HindII	GTYRAC	3 cut(s)	1548, 1936, 2947
HindIII	AAGCTT	5 cut(s)	1527, 1571, 1829, 3110, 3895
HpaII	CCGG	1 cut(s)	535
HphI	GGTGA	2 cut(s)	479, 974
Hpy166II	GTNNAC	8 cut(s)	1240, 1548, 1936, 2237, 2387, 2471, 2947, 3733
Hpy8I	GTNNAC	8 cut(s)	1240, 1548, 1936, 2237, 2387, 2471, 2947, 3733
Hpy99I	CGWCG	5 cut(s)	2081, 2280, 3042, 3627, 3630
HpyAV	CCTTC	9 cut(s)	542, 722, 785, 2060, 2107, 2608, 2830, 3282, 3648
Hsp92I	GRCGYC	2 cut(s)	3167, 3625
HspAI	GCGC	6 cut(s)	366, 368, 1600, 1954, 1992, 3580
KpnI	GGTACC	1 cut(s)	517
Ksp22I	TGATCA	2 cut(s)	429, 1131
LguI	GCTCTTC	1 cut(s)	2185
LmnI	GCTCC	9 cut(s)	181, 476, 692, 943, 1443, 2421, 2654, 3084, 3677
MaeI	CTAG	6 cut(s)	252, 527, 636, 2744, 3101, 3465
MaeIII	GTNAC	3 cut(s)	341, 852, 1088
MbiI	CCGCTC	1 cut(s)	3460
MfeI	CAATTG	1 cut(s)	1257
MflI	RGATCY	1 cut(s)	1480
MhlI	GDGCHC	3 cut(s)	1013, 2659, 3682
MlsI	TGGCCA	1 cut(s)	75
MluNI	TGGCCA	1 cut(s)	75
MlyI	GAGTC	5 cut(s)	44, 2360, 2978, 3299, 3728
MmeI	TCCRAC	3 cut(s)	907, 2700, 3273
Mox20I	TGGCCA	1 cut(s)	75
Mph1103I	ATGCAT	4 cut(s)	1102, 1114, 3267, 3368
MscI	TGGCCA	1 cut(s)	75
MslI	CAYNNNNRTG	2 cut(s)	500, 2291
Msp20I	TGGCCA	1 cut(s)	75
MspA1I	CMGCKG	3 cut(s)	2230, 2357, 3218
MspCI	CTTAAG	2 cut(s)	1061, 2660
MspI	CCGG	1 cut(s)	535
MunI	CAATTG	1 cut(s)	1257
Mva1269I	GAATGC	1 cut(s)	3505
MvnI	CGCG	2 cut(s)	368, 1191
NciI	CCSGG	1 cut(s)	536
NdeI	CATATG	1 cut(s)	3796
NlaIV	GGNNCC	7 cut(s)	515, 1295, 1710, 2874, 3615, 3679, 3876
NmuCI	GTSAC	3 cut(s)	341, 852, 1088
NsiI	ATGCAT	4 cut(s)	1102, 1114, 3267, 3368
NspI	RCATGY	2 cut(s)	1537, 2094
NspV	TTCGAA	1 cut(s)	2022
PaeI	GCATGC	1 cut(s)	2094
PagI	TCATGA	2 cut(s)	889, 1128
PalAI	GGCGCGCC	1 cut(s)	366
PauI	GCGCGC	1 cut(s)	366
PceI	AGGCCT	3 cut(s)	1761, 2960, 3400
PciSI	GCTCTTC	1 cut(s)	2185
PctI	GAATGC	1 cut(s)	3505
PfeI	GAWTC	8 cut(s)	578, 684, 1022, 2101, 2816, 2927, 3125, 3820
PflMI	CCANNNNNTGG	2 cut(s)	1684, 2902
PfoI	TCCNGGA	1 cut(s)	3081
PleI	GAGTC	5 cut(s)	44, 2359, 2977, 3298, 3728
PpsI	GAGTC	5 cut(s)	44, 2359, 2977, 3298, 3728
Ppu21I	YACGTR	2 cut(s)	1705, 2384
PpuMI	RGGWCCY	1 cut(s)	1294
PshBI	ATTAAT	2 cut(s)	306, 1353
PsiI	TTATAA	1 cut(s)	1607
Psp1406I	AACGTT	1 cut(s)	2728
Psp5II	RGGWCCY	1 cut(s)	1294
PspFI	CCCAGC	2 cut(s)	3618, 3763
PspN4I	GGNNCC	7 cut(s)	515, 1295, 1710, 2874, 3615, 3679, 3876
PspPI	GGNCC	3 cut(s)	1294, 1660, 3192
PspPPI	RGGWCCY	1 cut(s)	1294
PstI	CTGCAG	2 cut(s)	2215, 2941
PstNI	CAGNNNCTG	1 cut(s)	1951
PsuI	RGATCY	1 cut(s)	1480
PteI	GCGCGC	1 cut(s)	366
PvuII	CAGCTG	2 cut(s)	2357, 3218
RsaI	GTAC	5 cut(s)	515, 2202, 2209, 2238, 3761
RsaNI	GTAC	5 cut(s)	514, 2201, 2208, 2237, 3760
RseI	CAYNNNNRTG	2 cut(s)	500, 2291
SapI	GCTCTTC	1 cut(s)	2185
Sau96I	GGNCC	3 cut(s)	1294, 1660, 3192
ScaI	AGTACT	2 cut(s)	2202, 2209
SchI	GAGTC	5 cut(s)	44, 2360, 2978, 3299, 3728
SduI	GDGCHC	3 cut(s)	1013, 2659, 3682
SfcI	CTRYAG	4 cut(s)	1866, 2211, 2714, 2937
SfuI	TTCGAA	1 cut(s)	2022
SgsI	GGCGCGCC	1 cut(s)	366
SinI	GGWCC	3 cut(s)	1294, 1660, 3192
SmiMI	CAYNNNNRTG	2 cut(s)	500, 2291
SmlI	CTYRAG	4 cut(s)	33, 1061, 2561, 2660
SmoI	CTYRAG	4 cut(s)	33, 1061, 2561, 2660
SnaBI	TACGTA	1 cut(s)	2384
SphI	GCATGC	1 cut(s)	2094
SseBI	AGGCCT	3 cut(s)	1761, 2960, 3400
SspI	AATATT	2 cut(s)	994, 3697
SspMI	CTAG	6 cut(s)	252, 527, 636, 2744, 3101, 3465
StuI	AGGCCT	3 cut(s)	1761, 2960, 3400
StyI	CCWWGG	1 cut(s)	1005
TatI	WGTACW	2 cut(s)	2200, 2207
TauI	GCSGC	4 cut(s)	328, 1191, 2233, 2560
TfiI	GAWTC	8 cut(s)	578, 684, 1022, 2101, 2816, 2927, 3125, 3820
TscAI	CASTG	9 cut(s)	18, 469, 770, 2277, 2591, 2685, 2920, 3443, 3591
TseFI	GTSAC	3 cut(s)	341, 852, 1088
Tsp45I	GTSAC	3 cut(s)	341, 852, 1088
TspGWI	ACGGA	5 cut(s)	568, 769, 1643, 1970, 3662
TspRI	CASTG	9 cut(s)	18, 469, 770, 2277, 2591, 2685, 2920, 3443, 3591
Van91I	CCANNNNNTGG	2 cut(s)	1684, 2902
Vha464I	CTTAAG	2 cut(s)	1061, 2660
VpaK11BI	GGWCC	3 cut(s)	1294, 1660, 3192
VspI	ATTAAT	2 cut(s)	306, 1353
XapI	RAATTY	7 cut(s)	241, 650, 660, 2024, 2367, 3088, 3176
XceI	RCATGY	2 cut(s)	1537, 2094
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3895
XspI	CTAG	6 cut(s)	252, 527, 636, 2744, 3101, 3465
ZraI	GACGTC	1 cut(s)	3626
ZrmI	AGTACT	2 cut(s)	2202, 2209
Zsp2I	ATGCAT	4 cut(s)	1102, 1114, 3267, 3368

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.