MD04G1229800.v1.1

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0002633
Physical Location & Seq
Reverse (-)
30997445 .. 31004908
7464 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1229800.v1.1.491

Sequence Viewer

Length: 3648 bp
ATGGGGACAGAACTCATGAGAGTTTGCATGCAAGAGGATAACGATGAGTTTCCTTCTGTTCCACCCGGATTTGAGTCATTTGCATCTTTCAGCTTACAAAGGGCAAATGAAAGTGAGAAACAAGATAGTGAAAACAGAATTAGTTCCTCAGCAACTGCTAGTCCTTCTAAATCACAATCAGTTCAGATGGAGACTGACATTGACACTGGTGGGGTTGCAAAGAGGTCTGTTCGACGCAGACAATGCATAAATCATGGGCAAAATAACAAGTCAGAGGATGAATCTGATTGGGAGCAACTTGAACAAAATTGTCCCCCAAGATCTGTTCTTCCCAAGGGGGTTATACGTGGGTGTCCACAATGTAGTAATTGCCAAAAGGTCTCTGCACGATGGCATCCACTGGAAGGTCAAAGGCCCGACCTTCAGGACGCTCCTGTCTTCAGGCCTTCTGAGGAGGAGTTCAAAGACACATTGAAATATATAGCGAGCATACGCCCAAAAGCTGAACCATATGGTATTTGTCGCATTGTTCCTCCCTCTTCTTGGAAGCCTCCCTGTCCACTCAAGGAAAAGGCTATTTGGGGGACCTCCACATTTTTTACTCGAGTCCAGAGAGTCGATAAACTGCAAAATCGAGATTCAATGAGAAAGGTTCCTAAAAGCCATAGTCATATGAGGAAGAAAAGAAGAAGCACACGGATGGGGACTGATCATCAAAGTGGTGGCAGAGGATCTGGGGATGATGGAGGTTGTGAGGCCGAACGGTTTGGGTTTGAACCTGGTCCAGAGTTTACTCTAGAAACGTTTGAAAGATATGCTGACGACTTCAAGGCTCAATACTTCAGCAAGAACGAACATATTCCTAGTATAGGAGGTAACTTTGCTAAACTTAAAGAGTGCTGGGAGCCATCGGTAGGAAGTATTGAGGGCGAATATTGGCGGATGGTAGAGAGACCTACTGAAGAAATTGAGGTTCTTTATGGTGCTGATCTAGAAACTGGAGTTTTTGGTAGTGGTTTTCCCAAGATGTCCAGTAAGGATGCTTTTCCTTCAGAAGAGCAGTACATAAATTCGGGCTGGAACTTGAACAACTTTCCAAGACTTCCCGGATCTGTTCTTTCTTATGAAAGCAGTGATATATCCGGTGTTCTGGTGCCATGGTTATACATAGGGATGTGCTTTTCTTCCTTTTGCTGGCATGTTGAAGATCACCACTTGTACTCTTTAAATTATATGCATTGGGGTGCTCCAAAGTTGTGGTATGGTATCCCTGGTAGCGATGCCTGTAAATTTGAAGAAGCAATGAGAAAGCACCTCCCTGGCCTATTTGAAGAGCAACCTGACTTGCTTCATAAGCTGGTCACACAGCTTTCACCCTCCATTCTGAAATCTGAAGGGGTACCTGTGTATAGATGTTGTCAGAATGCTGGAGAGTTTGTTTTGACCTTTCCTCGAGCATACCATTCAGGGTTCAACTGTGGTTTTAATTGTGCCGAGGCTGTGAATGTAGCTCCTGTTGATTGGTTGCCCCATGGGCAGATCGCTATAGAGCTATATCAGGAGCAGGGACGAAAGACATCCATTTCTCATGATAAATTGTTGCTTGGGGCAGCAAGGGAAGCGGTGAAAGCACATTGGGAACTCAATTTACTGAAGAAGAATACTCCAGATAACTTACGATGGAAGGATGCATGTGGAAAGGATGGGATATTAGCAAAAACACTAAAGGCACGTGTTGAGATGGAGCGTGTGAGGAGAGAATTTCTCACTAGTTCTTCACAGGCATTGAAGATGGATAACAATTTTGATGCCATCAATGAGAGAGAATGCAGCATATGCTTTTTTGATCTTCATCTGTCAGCAGCTGGTTGTCATCATTGTTCCCCGGATAGATATGCATGCTTAAATCATGCAAAGAAATTCTGCTCATGTGCTTGGAGCGCCAAGTTTTTCCTCTTTCGTTATGATATGGATGAACTAAACATCCTTCTTGAGGCATTGGAAGGAAAGTTGAGTGCAGTATACAGATGGGCTCGACTCGATCTTGGGCTGGCTCTGAGTTCTTATATTTCCAAAGACAATAAAGTTGGTAAGATATCTTATTCCTCCAGAAGTGATATACTTAAAGAGGTTAGCTCAGAGCTGCAAAGCAAAAACTTCAAAGATCCTCTAGGTACAGAAATCCATAAAGAGAGTCCTATGAGTTCAGCCGGGATCATTGGTGAGACTTCCTCGCAGCAGAAGGTGAAAACATTAGAAATTTTTCGTCAAGTTAAAAGAGAAGAATCGGCTCTCAACAGTTCCAAGTCAAGAATGCAGGTTTGCCAGTTGTCTCAAGAGGACATGTCATATGCTGTGACCTCGGATGCAAAGGTATCTGGGACGAATATGGCTTCAGTTGAGGATGTTATACTTCTTAGCGATGATGAAGGAGACGATGACCCAAAGAAGCCACTTTCAACTGTGAGTGACCCTGGTTTTGAGAAAGAACCAATCTTGAATATACCAGGGACTGATGCAGCAGTTATGGGTGAAAAGGTTTTCAGTCCATTACCTGGTGGAGAAAAAAAGGATTCTTCATCTCATCCTGTACACGTGAAAGTTGAACAGGATCATGGTGAACAATTAGGTTCTAATCCACCAAACCTCTCTTTCAAATTAGTTTCTACTAAAGCAGAATGTGGCCCAAACGGTAGTGCCATCTTTGAACACAAAGTTGCTAATTCAAGGAGTGACCCTCAGCTTCCACAGCCATGTGCCAGTATAAAACTAGAGAATGAGGACAAATATGAAAAGATAGGAAGAATTGCCGATACAACTTTAGCAGACAATGTACGAACGACAACTGGAGCCTCATCGTCCAGCCAAAACAATTTGGACCGATATTACCGCCAGAAGGGTCCCCGAATAGCGAAGGTGGTGCGGAGGATCTCCTGCATTGTTGAGCCTCTGGAAATTGGAGTCGTGCTATCTGGAAAGTCATGGTGTAACAGCCAAGCCATTTTTCCAAAAGGATTTAGAAGCCGGGTTAGGCACATCAGTGTTCTGGATCCAACTGTTAGGTGTCACTATGTCTCAGAAGTACTGGATGCTGGACAAGGTGGACCACTGTTCAAGGTTTCTTTGGAGCACTGTCCAGGTGAGGTTTTCATACATAATTCAGCTGGAAGATGCTGGGAAATGGTGAGAGAGAGAGTCAATCAAGAAATCACTAGGCAACATAAATTGGGAAAAATGAACCTTCCTCCTTTGCAGCCACCGGGGAGTATCGATGGCTTTGAAATGTTTGGGTTTACTTCACCAGCTATTGTACAGGCCATAGAGGCAATGGACCGAAATCATGTCTGTTCCGAGTACTGGGACTCTCGCCCCTATTCCCGACCTCAAGTACAGATTCCACAAAAACCTCAATCCAAAGAAAGTAGTGAGAGCTGCAATACAATGTCAAAGGAAAAAAGTGATCAAGAGGCATCCGACAATGATCTCGTCCCTGCTGGAGTCGATACAACACTCAGAAGCCTATTCAAGAAGGCTAACTTGGACGAATTAAACTCACTGCACAGCATTTTAGGTGCGAATCGGCAACCTGCTGGTCGAGGTCTAGTGACACGACTTCTTAATGAAGAGATTCATAGTCGTCCCACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000976 GO:0001067 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0003824 GO:0005488 GO:0005506 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006325 GO:0006355 GO:0006464 GO:0006479 GO:0006482 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008198 GO:0008213 GO:0008214 GO:0008276 GO:0009314 GO:0009416 GO:0009628 GO:0009648 GO:0009791 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009910 GO:0009987 GO:0010216 GO:0010228 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0016577 GO:0016740 GO:0016741 GO:0019219 GO:0019222 GO:0019538 GO:0022414 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0032451 GO:0032452 GO:0032453 GO:0032501 GO:0032502 GO:0034641 GO:0034720 GO:0036211 GO:0040008 GO:0040009 GO:0040010 GO:0040029 GO:0042054 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045814 GO:0045892 GO:0045927 GO:0045934 GO:0046483 GO:0046872 GO:0046914 GO:0048518 GO:0048519 GO:0048523 GO:0048573 GO:0048579 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048586 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051172 GO:0051239 GO:0051241 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0140096 GO:0140110 GO:1901360 GO:1901363 GO:1901564 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000026 GO:2000028 GO:2000112 GO:2000113 GO:2000241 GO:2000242 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FYRC FYRN JmjC JmjN zf-C5HC2

Protein Analysis

1216

Amino Acids

135.97

Weight (kDa)

6.36

Isoelectric Point (pI)

54.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JmjN PF02375 145 - 178 1.3e-17 jmjN domain
JmjC PF02373 387 - 503 7.8e-46 JmjC domain, hydroxylase
zf-C5HC2 PF02928 610 - 662 5.3e-16 C5HC2 zinc finger
FYRN PF05964 985 - 1030 2.8e-09 F/Y-rich N-terminus
FYRC PF05965 1035 - 1119 4.7e-23 F/Y rich C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 434
Acc36I ACCTGC 2 cut(s) 2308, 3595
Acc65I GGTACC 1 cut(s) 1399
AccB1I GGYRCC 2 cut(s) 1153, 1399
AccB7I CCANNNNNTGG 1 cut(s) 2555
AccI GTMKAC 1 cut(s) 2022
AciI CCGC 4 cut(s) 940, 1622, 2890, 2923
AclI AACGTT 1 cut(s) 803
AclWI GGATC 8 cut(s) 739, 1117, 2159, 2222, 2619, 2936, 3044, 3057
AcsI RAATTY 5 cut(s) 1069, 1289, 1760, 1919, 2259
AcuI CTGAAG 8 cut(s) 407, 424, 826, 981, 1035, 1413, 1673, 2379
AcvI CACGTG 2 cut(s) 1733, 2596
AfiI CCNNNNNNNGG 9 cut(s) 404, 543, 869, 914, 1194, 1993, 2555, 2896, 3357
AflIII ACRYGT 3 cut(s) 1732, 2343, 2593
AhlI ACTAGT 1 cut(s) 1769
AjnI CCWGG 7 cut(s) 778, 1270, 1318, 2473, 2506, 2554, 3136
AjuI GAANNNNNNNTTGG 4 cut(s) 2988, 3020, 3521, 3553
AleI CACNNNNGTG 1 cut(s) 3039
AloI GAACNNNNNNTCC 2 cut(s) 1968, 2000
Alw21I GWGCWC 2 cut(s) 1249, 3132
Alw26I GTCTC 7 cut(s) 185, 385, 946, 2219, 2337, 2427, 3079
AlwI GGATC 8 cut(s) 739, 1117, 2159, 2222, 2619, 2936, 3044, 3057
AlwNI CAGNNNCTG 3 cut(s) 155, 1865, 2513
Ama87I CYCGRG 2 cut(s) 603, 1452
AoxI GGCC 6 cut(s) 413, 443, 756, 1321, 2683, 3315
ApeKI GCWGC 8 cut(s) 1610, 1830, 1862, 2143, 2236, 2519, 3253, 3432
ApoI RAATTY 5 cut(s) 1069, 1289, 1760, 1919, 2259
ArsI GACNNNNNNTTYG 2 cut(s) 2462, 2494
Asp700I GAANNNNTTC 4 cut(s) 142, 858, 2262, 3627
Asp718I GGTACC 1 cut(s) 1399
AspLEI GCGC 1 cut(s) 1943
AspS9I GGNCC 8 cut(s) 414, 585, 782, 2684, 2878, 2900, 3104, 3331
AsuC2I CCSGG 6 cut(s) 66, 1107, 1886, 2212, 3026, 3261
AvaI CYCGRG 2 cut(s) 603, 1452
AvaII GGWCC 6 cut(s) 585, 782, 2878, 2900, 3104, 3331
BaeI ACNNNNGTAYC 2 cut(s) 1391, 1424
BamHI GGATCC 1 cut(s) 3049
BanI GGYRCC 2 cut(s) 1153, 1399
BanII GRGCYC 1 cut(s) 2035
BbrPI CACGTG 2 cut(s) 1733, 2596
BbsI GAAGAC 1 cut(s) 430
Bbv12I GWGCWC 2 cut(s) 1249, 3132
BbvCI CCTCAGC 2 cut(s) 148, 2739
BbvI GCAGC 8 cut(s) 1622, 1842, 1874, 2130, 2248, 2531, 3265, 3419
BcgI CGANNNNNNTGC 2 cut(s) 2902, 2936
BciT130I CCWGG 7 cut(s) 780, 1272, 1320, 2475, 2508, 2556, 3138
BciVI GTATCC 1 cut(s) 1277
BclI TGATCA 2 cut(s) 709, 3460
BcnI CCSGG 6 cut(s) 66, 1107, 1886, 2212, 3026, 3261
BcoDI GTCTC 7 cut(s) 185, 385, 946, 2219, 2337, 2427, 3079
BcuI ACTAGT 1 cut(s) 1769
BfaI CTAG 9 cut(s) 159, 797, 864, 992, 1770, 2171, 2771, 3213, 3602
BfmI CTRYAG 1 cut(s) 1545
BfoI RGCGCY 1 cut(s) 1944
BfuAI ACCTGC 2 cut(s) 2308, 3595
BfuI GTATCC 1 cut(s) 1277
BglI GCCNNNNNGGC 2 cut(s) 1534, 3323
BglII AGATCT 1 cut(s) 320
BisI GCNGC 8 cut(s) 1611, 1831, 1863, 2144, 2237, 2520, 3254, 3433
BlsI GCNGC 8 cut(s) 1612, 1832, 1864, 2145, 2238, 2521, 3255, 3434
BmcAI AGTACT 2 cut(s) 3084, 3356
Bme18I GGWCC 6 cut(s) 585, 782, 2878, 2900, 3104, 3331
BmeT110I CYCGRG 2 cut(s) 603, 1452
BmgT120I GGNCC 8 cut(s) 414, 585, 782, 2684, 2878, 2900, 3104, 3331
BmiI GGNNCC 9 cut(s) 586, 654, 906, 1155, 1401, 2851, 2901, 2902, 3051
BmrI ACTGGG 1 cut(s) 3367
BmuI ACTGGG 1 cut(s) 3367
BpiI GAAGAC 1 cut(s) 430
BplI GAGNNNNNCTC 8 cut(s) 1749, 1781, 2120, 2152, 2216, 2248, 2722, 2754
BpmI CTGGAG 6 cut(s) 1020, 1449, 1650, 2092, 2868, 3516
Bpu10I CCTNAGC 2 cut(s) 148, 2739
BpuEI CTTGAG 4 cut(s) 548, 2012, 2319, 3369
BpuMI CCSGG 6 cut(s) 66, 1107, 1886, 2212, 3026, 3261
Bsa29I ATCGAT 1 cut(s) 3270
BsaAI YACGTR 3 cut(s) 347, 1733, 2596
BsaBI GATNNNNATC 1 cut(s) 1851
BsaI GGTCTC 2 cut(s) 385, 946
BsaWI WCCGGW 1 cut(s) 1142
BsaXI ACNNNNNCTCC 2 cut(s) 1422, 1452
Bsc4I CCNNNNNNNGG 9 cut(s) 404, 543, 869, 914, 1194, 1993, 2555, 2896, 3357
Bse1I ACTGG 9 cut(s) 211, 405, 1003, 1032, 2326, 2760, 2851, 3090, 3362
Bse3DI GCAATG 2 cut(s) 1308, 3333
Bse8I GATNNNNATC 1 cut(s) 1851
BseBI CCWGG 7 cut(s) 780, 1272, 1320, 2475, 2508, 2556, 3138
BseCI ATCGAT 1 cut(s) 3270
BseJI GATNNNNATC 1 cut(s) 1851
BseLI CCNNNNNNNGG 9 cut(s) 404, 543, 869, 914, 1194, 1993, 2555, 2896, 3357
BseMI GCAATG 2 cut(s) 1308, 3333
BseMII CTCAG 7 cut(s) 162, 441, 2048, 2151, 2753, 3090, 3526
BseNI ACTGG 9 cut(s) 211, 405, 1003, 1032, 2326, 2760, 2851, 3090, 3362
BseRI GAGGAG 3 cut(s) 467, 470, 1768
BseXI GCAGC 8 cut(s) 1622, 1842, 1874, 2130, 2248, 2531, 3265, 3419
BseYI CCCAGC 2 cut(s) 900, 3174
BsgI GTGCAG 3 cut(s) 369, 2037, 3542
BshFI GGCC 6 cut(s) 415, 445, 758, 1323, 2685, 3317
BshNI GGYRCC 2 cut(s) 1153, 1399
BshVI ATCGAT 1 cut(s) 3270
BsiHKAI GWGCWC 2 cut(s) 1249, 3132
BsiHKCI CYCGRG 2 cut(s) 603, 1452
BsiSI CCGG 7 cut(s) 66, 1107, 1143, 1886, 2211, 3025, 3260
BslI CCNNNNNNNGG 9 cut(s) 404, 543, 869, 914, 1194, 1993, 2555, 2896, 3357
BsmAI GTCTC 7 cut(s) 185, 385, 946, 2219, 2337, 2427, 3079
BsmBI CGTCTC 1 cut(s) 2427
BsmI GAATGC 3 cut(s) 1429, 1832, 2319
BsnI GGCC 6 cut(s) 415, 445, 758, 1323, 2685, 3317
Bso31I GGTCTC 2 cut(s) 385, 946
BsoBI CYCGRG 2 cut(s) 603, 1452
Bsp1286I GDGCHC 3 cut(s) 1249, 2035, 3132
Bsp1407I TGTACA 2 cut(s) 2590, 3310
Bsp19I CCATGG 2 cut(s) 1157, 1531
BspACI CCGC 4 cut(s) 940, 1622, 2890, 2923
BspANI GGCC 6 cut(s) 415, 445, 758, 1323, 2685, 3317
BspCNI CTCAG 7 cut(s) 161, 442, 2049, 2150, 2752, 3089, 3525
BspDI ATCGAT 1 cut(s) 3270
BspHI TCATGA 2 cut(s) 15, 1588
BspLI GGNNCC 9 cut(s) 586, 654, 906, 1155, 1401, 2851, 2901, 2902, 3051
BspMI ACCTGC 2 cut(s) 2308, 3595
BspPI GGATC 8 cut(s) 739, 1117, 2159, 2222, 2619, 2936, 3044, 3057
BspQI GCTCTTC 2 cut(s) 1050, 1326
BspT107I GGYRCC 2 cut(s) 1153, 1399
BspTNI GGTCTC 2 cut(s) 385, 946
BsrDI GCAATG 2 cut(s) 1308, 3333
BsrGI TGTACA 2 cut(s) 2590, 3310
BsrI ACTGG 9 cut(s) 211, 405, 1003, 1032, 2326, 2760, 2851, 3090, 3362
BssNAI GTATAC 1 cut(s) 2023
BssT1I CCWWGG 3 cut(s) 333, 1157, 1531
Bst1107I GTATAC 1 cut(s) 2023
Bst2UI CCWGG 7 cut(s) 780, 1272, 1320, 2475, 2508, 2556, 3138
Bst4CI ACNGT 8 cut(s) 765, 1478, 2300, 2464, 2693, 3058, 3111, 3134
Bst6I CTCTTC 4 cut(s) 544, 1050, 1326, 3618
BstAPI GCANNNNNTGC 2 cut(s) 243, 1836
BstAUI TGTACA 2 cut(s) 2590, 3310
BstBAI YACGTR 3 cut(s) 347, 1733, 2596
BstC8I GCNNGC 5 cut(s) 29, 487, 1196, 1900, 2052
BstDEI CTNAG 8 cut(s) 148, 450, 2057, 2137, 2417, 2739, 3076, 3512
BstDSI CCRYGG 2 cut(s) 1157, 1531
BstENI CCTNNNNNAGG 2 cut(s) 867, 1991
BstH2I RGCGCY 1 cut(s) 1944
BstHHI GCGC 1 cut(s) 1943
BstMAI GTCTC 7 cut(s) 185, 385, 946, 2219, 2337, 2427, 3079
BstMWI GCNNNNNNNGC 9 cut(s) 243, 1354, 1534, 1619, 1628, 1836, 1940, 2749, 3323
BstNI CCWGG 7 cut(s) 780, 1272, 1320, 2475, 2508, 2556, 3138
BstNSI RCATGY 5 cut(s) 31, 1202, 1695, 1902, 2347
BstSFI CTRYAG 1 cut(s) 1545
BstV1I GCAGC 8 cut(s) 1622, 1842, 1874, 2130, 2248, 2531, 3265, 3419
BstV2I GAAGAC 1 cut(s) 430
BstX2I RGATCY 6 cut(s) 320, 731, 1109, 2164, 2928, 3049
BstXI CCANNNNNNTGG 1 cut(s) 1257
BstYI RGATCY 6 cut(s) 320, 731, 1109, 2164, 2928, 3049
BstZ17I GTATAC 1 cut(s) 2023
Bsu15I ATCGAT 1 cut(s) 3270
BsuI GTATCC 1 cut(s) 1277
BsuRI GGCC 6 cut(s) 415, 445, 758, 1323, 2685, 3317
BsuTUI ATCGAT 1 cut(s) 3270
BtgI CCRYGG 2 cut(s) 1157, 1531
BtgZI GCGATG 2 cut(s) 1293, 2436
BtsI GCAGTG 2 cut(s) 1138, 3554
BtsIMutI CAGTG 7 cut(s) 204, 398, 1138, 3046, 3107, 3130, 3554
BveI ACCTGC 2 cut(s) 2308, 3595
Cac8I GCNNGC 5 cut(s) 29, 487, 1196, 1900, 2052
CaiI CAGNNNCTG 3 cut(s) 155, 1865, 2513
CciI TCATGA 2 cut(s) 15, 1588
CfoI GCGC 1 cut(s) 1943
Cfr13I GGNCC 8 cut(s) 414, 585, 782, 2684, 2878, 2900, 3104, 3331
ClaI ATCGAT 1 cut(s) 3270
CseI GACGC 2 cut(s) 243, 437
CsiI ACCWGGT 2 cut(s) 778, 2554
DdeI CTNAG 8 cut(s) 148, 450, 2057, 2137, 2417, 2739, 3076, 3512
DraI TTTAAA 1 cut(s) 1227
DrdI GACNNNNNNGTC 1 cut(s) 434
DseDI GACNNNNNNGTC 1 cut(s) 434
Eam1104I CTCTTC 4 cut(s) 544, 1050, 1326, 3618
EarI CTCTTC 4 cut(s) 544, 1050, 1326, 3618
EciI GGCGGA 1 cut(s) 955
Eco130I CCWWGG 3 cut(s) 333, 1157, 1531
Eco147I AGGCCT 1 cut(s) 445
Eco24I GRGCYC 1 cut(s) 2035
Eco31I GGTCTC 2 cut(s) 385, 946
Eco32I GATATC 1 cut(s) 2097
Eco47I GGWCC 6 cut(s) 585, 782, 2878, 2900, 3104, 3331
Eco57I CTGAAG 8 cut(s) 407, 424, 826, 981, 1035, 1413, 1673, 2379
Eco72I CACGTG 2 cut(s) 1733, 2596
Eco88I CYCGRG 2 cut(s) 603, 1452
EcoNI CCTNNNNNAGG 2 cut(s) 867, 1991
EcoO109I RGGNCCY 2 cut(s) 585, 2900
EcoRII CCWGG 7 cut(s) 778, 1270, 1318, 2473, 2506, 2554, 3136
EcoRV GATATC 1 cut(s) 2097
EcoT14I CCWWGG 3 cut(s) 333, 1157, 1531
EcoT22I ATGCAT 4 cut(s) 248, 1239, 1693, 1900
EcoT38I GRGCYC 1 cut(s) 2035
ErhI CCWWGG 3 cut(s) 333, 1157, 1531
Esp3I CGTCTC 1 cut(s) 2427
FalI AAGNNNNNCTT 2 cut(s) 3521, 3553
FauNDI CATATG 4 cut(s) 511, 672, 1835, 2350
FbaI TGATCA 2 cut(s) 709, 3460
FblI GTMKAC 1 cut(s) 2022
Fnu4HI GCNGC 8 cut(s) 1611, 1831, 1863, 2144, 2237, 2520, 3254, 3433
FriOI GRGCYC 1 cut(s) 2035
Fsp4HI GCNGC 8 cut(s) 1611, 1831, 1863, 2144, 2237, 2520, 3254, 3433
FspBI CTAG 9 cut(s) 159, 797, 864, 992, 1770, 2171, 2771, 3213, 3602
GlaI GCGC 1 cut(s) 1942
GluI GCNGC 8 cut(s) 1611, 1831, 1863, 2144, 2237, 2520, 3254, 3433
GsaI CCCAGC 2 cut(s) 904, 3178
GsuI CTGGAG 6 cut(s) 1020, 1449, 1650, 2092, 2868, 3516
HaeII RGCGCY 1 cut(s) 1944
HaeIII GGCC 6 cut(s) 415, 445, 758, 1323, 2685, 3317
HapII CCGG 7 cut(s) 66, 1107, 1143, 1886, 2211, 3025, 3260
HgaI GACGC 2 cut(s) 243, 437
HhaI GCGC 1 cut(s) 1943
Hin6I GCGC 1 cut(s) 1941
HinP1I GCGC 1 cut(s) 1941
HpaII CCGG 7 cut(s) 66, 1107, 1143, 1886, 2211, 3025, 3260
Hpy166II GTNNAC 8 cut(s) 356, 560, 792, 2023, 2593, 2621, 3104, 3294
Hpy8I GTNNAC 8 cut(s) 356, 560, 792, 2023, 2593, 2621, 3104, 3294
Hpy99I CGWCG 1 cut(s) 237
HpyCH4III ACNGT 8 cut(s) 765, 1478, 2300, 2464, 2693, 3058, 3111, 3134
HpyCH4IV ACGT 4 cut(s) 346, 803, 1732, 2595
HpyF10VI GCNNNNNNNGC 9 cut(s) 243, 1354, 1534, 1619, 1628, 1836, 1940, 2749, 3323
HpyF3I CTNAG 8 cut(s) 148, 450, 2057, 2137, 2417, 2739, 3076, 3512
HpySE526I ACGT 4 cut(s) 346, 803, 1732, 2595
HspAI GCGC 1 cut(s) 1941
KflI GGGWCCC 1 cut(s) 2900
KpnI GGTACC 1 cut(s) 1403
Ksp22I TGATCA 2 cut(s) 709, 3460
LguI GCTCTTC 2 cut(s) 1050, 1326
Lsp1109I GCAGC 8 cut(s) 1622, 1842, 1874, 2130, 2248, 2531, 3265, 3419
MabI ACCWGGT 2 cut(s) 778, 2554
MaeI CTAG 9 cut(s) 159, 797, 864, 992, 1770, 2171, 2771, 3213, 3602
MaeII ACGT 4 cut(s) 346, 803, 1732, 2595
MaeIII GTNAC 8 cut(s) 875, 1360, 2356, 2468, 2732, 2987, 3065, 3604
MflI RGATCY 6 cut(s) 320, 731, 1109, 2164, 2928, 3049
MhlI GDGCHC 3 cut(s) 1249, 2035, 3132
MlyI GAGTC 9 cut(s) 83, 615, 624, 2031, 2203, 2970, 3204, 3356, 3507
MmeI TCCRAC 2 cut(s) 3077, 3498
Mph1103I ATGCAT 4 cut(s) 248, 1239, 1693, 1900
MroXI GAANNNNTTC 4 cut(s) 142, 858, 2262, 3627
MseI TTAA 8 cut(s) 891, 1226, 1485, 1904, 2124, 2274, 3548, 3618
MslI CAYNNNNRTG 6 cut(s) 698, 717, 1172, 1242, 2752, 3039
MspA1I CMGCKG 2 cut(s) 1865, 3164
MspI CCGG 7 cut(s) 66, 1107, 1143, 1886, 2211, 3025, 3260
Mva1269I GAATGC 3 cut(s) 1429, 1832, 2319
MvaI CCWGG 7 cut(s) 780, 1272, 1320, 2475, 2508, 2556, 3138
MwoI GCNNNNNNNGC 9 cut(s) 243, 1354, 1534, 1619, 1628, 1836, 1940, 2749, 3323
NciI CCSGG 6 cut(s) 66, 1107, 1886, 2212, 3026, 3261
NcoI CCATGG 2 cut(s) 1157, 1531
NdeI CATATG 4 cut(s) 511, 672, 1835, 2350
NlaIV GGNNCC 9 cut(s) 586, 654, 906, 1155, 1401, 2851, 2901, 2902, 3051
NmeAIII GCCGAG 1 cut(s) 1519
NmuCI GTSAC 6 cut(s) 1360, 2356, 2468, 2732, 3065, 3604
NsiI ATGCAT 4 cut(s) 248, 1239, 1693, 1900
NspI RCATGY 5 cut(s) 31, 1202, 1695, 1902, 2347
OliI CACNNNNGTG 1 cut(s) 3039
PaeI GCATGC 2 cut(s) 31, 1902
PaeR7I CTCGAG 2 cut(s) 603, 1452
PagI TCATGA 2 cut(s) 15, 1588
PceI AGGCCT 1 cut(s) 445
PciI ACATGT 1 cut(s) 2343
PciSI GCTCTTC 2 cut(s) 1050, 1326
PctI GAATGC 3 cut(s) 1429, 1832, 2319
PdmI GAANNNNTTC 4 cut(s) 142, 858, 2262, 3627
PfeI GAWTC 7 cut(s) 281, 638, 2285, 2573, 3394, 3577, 3628
PflMI CCANNNNNTGG 1 cut(s) 2555
PfoI TCCNGGA 1 cut(s) 1105
PkrI GCNGC 8 cut(s) 1612, 1832, 1864, 2145, 2238, 2521, 3255, 3434
PleI GAGTC 9 cut(s) 82, 614, 623, 2031, 2202, 2969, 3203, 3356, 3506
PmaCI CACGTG 2 cut(s) 1733, 2596
PmlI CACGTG 2 cut(s) 1733, 2596
PpsI GAGTC 9 cut(s) 82, 614, 623, 2031, 2202, 2969, 3203, 3356, 3506
Ppu21I YACGTR 3 cut(s) 347, 1733, 2596
PpuMI RGGWCCY 2 cut(s) 585, 2900
PscI ACATGT 1 cut(s) 2343
Psp1406I AACGTT 1 cut(s) 803
Psp5II RGGWCCY 2 cut(s) 585, 2900
Psp6I CCWGG 7 cut(s) 778, 1270, 1318, 2473, 2506, 2554, 3136
PspCI CACGTG 2 cut(s) 1733, 2596
PspFI CCCAGC 2 cut(s) 900, 3174
PspGI CCWGG 7 cut(s) 778, 1270, 1318, 2473, 2506, 2554, 3136
PspN4I GGNNCC 9 cut(s) 586, 654, 906, 1155, 1401, 2851, 2901, 2902, 3051
PspPI GGNCC 8 cut(s) 414, 585, 782, 2684, 2878, 2900, 3104, 3331
PspPPI RGGWCCY 2 cut(s) 585, 2900
PspXI VCTCGAGB 2 cut(s) 603, 1452
PsrI GAACNNNNNNTAC 2 cut(s) 1632, 1664
PstNI CAGNNNCTG 3 cut(s) 155, 1865, 2513
PsuI RGATCY 6 cut(s) 320, 731, 1109, 2164, 2928, 3049
PvuII CAGCTG 2 cut(s) 1865, 3164
RseI CAYNNNNRTG 6 cut(s) 698, 717, 1172, 1242, 2752, 3039
SapI GCTCTTC 2 cut(s) 1050, 1326
SaqAI TTAA 8 cut(s) 891, 1226, 1485, 1904, 2124, 2274, 3548, 3618
SatI GCNGC 8 cut(s) 1611, 1831, 1863, 2144, 2237, 2520, 3254, 3433
Sau96I GGNCC 8 cut(s) 414, 585, 782, 2684, 2878, 2900, 3104, 3331
ScaI AGTACT 2 cut(s) 3084, 3356
SchI GAGTC 9 cut(s) 83, 615, 624, 2031, 2203, 2970, 3204, 3356, 3507
SduI GDGCHC 3 cut(s) 1249, 2035, 3132
SexAI ACCWGGT 2 cut(s) 778, 2554
SfcI CTRYAG 1 cut(s) 1545
Sfr274I CTCGAG 2 cut(s) 603, 1452
SinI GGWCC 6 cut(s) 585, 782, 2878, 2900, 3104, 3331
SlaI CTCGAG 2 cut(s) 603, 1452
SmiMI CAYNNNNRTG 6 cut(s) 698, 717, 1172, 1242, 2752, 3039
SmlI CTYRAG 6 cut(s) 563, 603, 1452, 1991, 2334, 3384
SmoI CTYRAG 6 cut(s) 563, 603, 1452, 1991, 2334, 3384
SpeI ACTAGT 1 cut(s) 1769
SphI GCATGC 2 cut(s) 31, 1902
SseBI AGGCCT 1 cut(s) 445
SsiI CCGC 4 cut(s) 940, 1622, 2890, 2923
SspI AATATT 1 cut(s) 935
SspMI CTAG 9 cut(s) 159, 797, 864, 992, 1770, 2171, 2771, 3213, 3602
StuI AGGCCT 1 cut(s) 445
StyI CCWWGG 3 cut(s) 333, 1157, 1531
TaaI ACNGT 8 cut(s) 765, 1478, 2300, 2464, 2693, 3058, 3111, 3134
TaiI ACGT 4 cut(s) 349, 806, 1735, 2598
TaqII GACCGA 2 cut(s) 2895, 3348
TatI WGTACW 7 cut(s) 1062, 1218, 2590, 3082, 3310, 3354, 3388
TfiI GAWTC 7 cut(s) 281, 638, 2285, 2573, 3394, 3577, 3628
Tru1I TTAA 8 cut(s) 891, 1226, 1485, 1904, 2124, 2274, 3548, 3618
Tru9I TTAA 8 cut(s) 891, 1226, 1485, 1904, 2124, 2274, 3548, 3618
TscAI CASTG 7 cut(s) 211, 405, 1138, 3046, 3114, 3137, 3561
TseFI GTSAC 6 cut(s) 1360, 2356, 2468, 2732, 3065, 3604
TseI GCWGC 8 cut(s) 1610, 1830, 1862, 2143, 2236, 2519, 3253, 3432
Tsp45I GTSAC 6 cut(s) 1360, 2356, 2468, 2732, 3065, 3604
TspGWI ACGGA 1 cut(s) 712
TspRI CASTG 7 cut(s) 211, 405, 1138, 3046, 3114, 3137, 3561
Van91I CCANNNNNTGG 1 cut(s) 2555
VpaK11BI GGWCC 6 cut(s) 585, 782, 2878, 2900, 3104, 3331
XagI CCTNNNNNAGG 2 cut(s) 867, 1991
XapI RAATTY 5 cut(s) 1069, 1289, 1760, 1919, 2259
XbaI TCTAGA 2 cut(s) 796, 991
XceI RCATGY 5 cut(s) 31, 1202, 1695, 1902, 2347
XcmI CCANNNNNNNNNTGG 1 cut(s) 3325
XhoI CTCGAG 2 cut(s) 603, 1452
XmiI GTMKAC 1 cut(s) 2022
XmnI GAANNNNTTC 4 cut(s) 142, 858, 2262, 3627
XspI CTAG 9 cut(s) 159, 797, 864, 992, 1770, 2171, 2771, 3213, 3602
ZrmI AGTACT 2 cut(s) 3084, 3356
Zsp2I ATGCAT 4 cut(s) 248, 1239, 1693, 1900
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.