pycom04g20360

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0002633
Physical Location & Seq
Reverse (-)
21894105 .. 21898218
4114 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g20360.3

Sequence Viewer

Length: 2544 bp
ATGTGCTTTTCTTCCTTTTGCTGGCATGTTGAAGATCACCACTTGTACTCTATAAATTATATGCATTGGGGTGCTCCAAAGTTGTGGTACGGTATCCCTGGTAGTGATGCCTGTAAATTTGAAGAAGCAATGAGAAAGCACCTCCCTGGCCTATTTGAAGAGCAACCTGACTTGCTTCATAAGCTGATCACACAGCTTTCACCCTCCATTCTCAAGTCTGAAGGGGTACCTGTGTATAGATGTTGTCAGAATGCTGGAGAGTTTGTTTTAACCTTTCCTCGAGCATACCATTCAGGGTTCAACTGTGGTTTTAATTGTGCCGAGGCTGTGAATGTTGCTCCTGTGGATTGGTTGCCCCATGGGCAGATTGCTATAGAGCTATACCAGGAGCAGGGACGAAAGACATCCATTTCCCATGATAAATTGTTGCTTGGGGCAGCAAGGGAAGCAGTGAAAGCACATTGGGAACTCAATTTACTCAAGAAGAATACTCCTGATAACTTACGATGGAAGGATGCATGTGGAAAGGATGGGATATTAGCAAAAACACTCAAGGCACGTGTTGAGATGGAGCGTGTGAGGAGAGAATTTCTCACTAGTTCTTCACAGGCGTTGAAGATGGATAACAATTTTGATGCCATCAATGAGAGGGAATGCAGCATATGCTTTTTTGATCTTCATCTGTCAGCAGCTGGTTGTCATCATTGTTCCCCGGATAGATATGCATGCTTAAATCATGCAAAGAAATTCTGCTCATGTGCTTGGAGCGCCAAGTTTTTCCTCTTTCGTTATGATATGGATGAACTAAACATCCTTCTTGAGGCATTGGAAGGAAAGTTGAGTGCAGTATACAGATGGGCTAGACTCGATCTTGGGCTGGCTTTGAGTTCTTATATTTCCAAAGACAATAAAGTTGCTAAGATATCTTATTCCTCTAGAAGTGATATACTTCAAGAGGTTAGCTCACAGCTGCAAAGCAAACACTTCAAAGATCCTCTAGGAACAGAAATCTCTAAAGAGAGTCCTATGAGTTCAGCCGCGATCATTGGTGAGACTTCCTCGCGGCAGAAGATGAAAACATTAGAAACTTTTCCTCAAGTTAAAAGAGAAGAATCAGCTGTTAACAGTTCCAAGTCAAGAATGCAGGTTTGCCAGTTGTCTCAAGAGGACATGTCATATGCTGTGACCTCAGATGCAAAGGTATCTGGTACGAATATGGCTTCAGTTGAGGATGTTATACTTCTTAGTGATGATGAAGGAGACGATGACCCAAAGAAGCCACTTTCAGATAGTTCGAAAGAAATTTCTTCTGCTGGCCAGTTGGAACTTTCTAAGAGATTGATCAGTTTGGATGGAACTGTGAGTGCTCCTAATTCTGAGAAAGAACCAATCTTGAATATACCAGGGACTGATGCAGCAGTTATGGGTGAAAAAGGTTTCAGTCCATTACCTGGTGGAGAAAAAATGGATTCTTCGTCTCATCCTGTACACATGAAAGTTGAACAGGATCATGGTGAACAATTAGGATCTAATCCTCCAAATCTCTATTTCAAATTAGTTTCTACTAAAGCAGAATGTGGCCCAAACGCTAGTGCCATCATTGAACACAAAGTTGCTAATTCAAGGAGTGACCCTCAGCCTCCACAGCCATGTGCCAGTATAAAACTTGAGAATGAGGAGAAACATGAAAAGATAAGAATTGCTGATACAACTTCAGCAGACAATGTACGAACAACAACTGGAACCTCATCGTCCAGCCAAAACAATTTGGACCGATATTACCGCCAGAAGGGTCCCCGAATAGCAAAGGTGGTGCGGAGAATCTCCTGCATTGTTGAGCCTCTGGAAATTGGAGTTGTGATATCTGGAAAGTCATGGTGTAACAGCCAAGCCATTTTTCCAAAAGGATTTAGAAGCCGGGTTAGGCACATGAGTGTTCTGGATCCAACTGTTAGGTGTCACTATGTCTCAGAAGTACTGGATGCTGGACAGGGTGGACCACTGTTCAAGGTTTCTTTGGAGCACTGTCCAAGTGAGGTTTTCATACATAATTCAGCTGGAAGATGCTGGGAAATGGTGAGAGAGAGAGTCAATCAAGAAATCACTAGGCAACATAAATTGGGAAAAATGAACCTTCCTCCTTTGCAGCCACCGGGGAGTCTTGATGGCTTTGAAATGTTTGGGTTTACTTCACCAGCTATTGTACAGGCCATAGAGGCAATGGACCGAAATCATGTCTGTTCTGAGTATTGGGACTCTCGCCCCTATTCCCGACCTCAAGTACAGATTCCACAAAAACCTCAATCCAAAGAAAGTAGTGAGAACTACAATACAATGTCAAAGGAAAAAAGTGATCAAGAGGCCTCCGACAATAATCTCGTCCCTGCTGGAGTCGATACAACACTCCGAAGCCTATTCAAGAAGGCTAACTTAGACGAATTAAACTCACTGTACAGCATTTTAAGTGAGAATCGGCAACCTGCTGGTCGAGGCCTAGTGACACGACTTCTTAATGAAGAGATTCATAGTCGTCCCACATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000976 GO:0001067 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0003824 GO:0005488 GO:0005506 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006325 GO:0006355 GO:0006464 GO:0006479 GO:0006482 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008198 GO:0008213 GO:0008214 GO:0008276 GO:0009314 GO:0009416 GO:0009628 GO:0009648 GO:0009791 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009910 GO:0009987 GO:0010216 GO:0010228 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0016577 GO:0016740 GO:0016741 GO:0019219 GO:0019222 GO:0019538 GO:0022414 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0032451 GO:0032452 GO:0032453 GO:0032501 GO:0032502 GO:0034641 GO:0034720 GO:0036211 GO:0040008 GO:0040009 GO:0040010 GO:0040029 GO:0042054 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045814 GO:0045892 GO:0045927 GO:0045934 GO:0046483 GO:0046872 GO:0046914 GO:0048518 GO:0048519 GO:0048523 GO:0048573 GO:0048579 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048586 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051172 GO:0051239 GO:0051241 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0140096 GO:0140110 GO:1901360 GO:1901363 GO:1901564 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000026 GO:2000028 GO:2000112 GO:2000113 GO:2000241 GO:2000242 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FYRC FYRN JmjC JmjN zf-C5HC2

Protein Analysis

848

Amino Acids

94.53

Weight (kDa)

6.55

Isoelectric Point (pI)

55.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1135, 2488
Acc65I GGTACC 1 cut(s) 226
AccB1I GGYRCC 1 cut(s) 226
AccB7I CCANNNNNTGG 1 cut(s) 1451
AccI GTMKAC 1 cut(s) 849
AccII CGCG 2 cut(s) 1040, 1063
AciI CCGC 4 cut(s) 1038, 1063, 1783, 1816
AclWI GGATC 5 cut(s) 986, 1515, 1534, 1937, 1950
AcoI YGGCCR 1 cut(s) 1315
AcsI RAATTY 4 cut(s) 116, 587, 746, 1302
AcuI CTGAAG 3 cut(s) 240, 1206, 1698
AcvI CACGTG 1 cut(s) 560
AfiI CCNNNNNNNGG 5 cut(s) 21, 391, 820, 1451, 1789
AflIII ACRYGT 2 cut(s) 559, 1170
AhlI ACTAGT 1 cut(s) 596
AjnI CCWGG 5 cut(s) 97, 145, 384, 1402, 1450
AjuI GAANNNNNNNTTGG 2 cut(s) 1881, 1913
AleI CACNNNNGTG 1 cut(s) 1932
AloI GAACNNNNNNTCC 2 cut(s) 795, 827
AluBI AGCT 9 cut(s) 184, 196, 379, 692, 963, 970, 1118, 2057, 2198
AluI AGCT 9 cut(s) 184, 196, 379, 692, 963, 970, 1118, 2057, 2198
Alw21I GWGCWC 3 cut(s) 76, 1369, 2025
Alw26I GTCTC 5 cut(s) 1046, 1164, 1254, 1482, 1972
AlwI GGATC 5 cut(s) 986, 1515, 1534, 1937, 1950
AlwNI CAGNNNCTG 2 cut(s) 692, 1409
Ama87I CYCGRG 1 cut(s) 279
AoxI GGCC 6 cut(s) 148, 1315, 1579, 2208, 2361, 2491
ApeKI GCWGC 6 cut(s) 437, 657, 689, 970, 1415, 2146
ApoI RAATTY 4 cut(s) 116, 587, 746, 1302
Asp700I GAANNNNTTC 2 cut(s) 1089, 2520
Asp718I GGTACC 1 cut(s) 226
AspLEI GCGC 1 cut(s) 770
AspS9I GGNCC 5 cut(s) 1580, 1771, 1793, 1997, 2224
AsuC2I CCSGG 3 cut(s) 713, 1919, 2154
AsuHPI GGTGA 7 cut(s) 29, 192, 1061, 1439, 1526, 2089, 2184
AsuII TTCGAA 1 cut(s) 1295
AvaI CYCGRG 1 cut(s) 279
AvaII GGWCC 4 cut(s) 1771, 1793, 1997, 2224
BaeI ACNNNNGTAYC 2 cut(s) 218, 251
BalI TGGCCA 1 cut(s) 1317
BamHI GGATCC 1 cut(s) 1942
BanI GGYRCC 1 cut(s) 226
BbrPI CACGTG 1 cut(s) 560
Bbv12I GWGCWC 3 cut(s) 76, 1369, 2025
BbvCI CCTCAGC 1 cut(s) 1635
BbvI GCAGC 6 cut(s) 449, 669, 701, 957, 1427, 2158
BccI CCATC 9 cut(s) 501, 524, 562, 613, 647, 849, 1346, 1604, 2159
BciT130I CCWGG 5 cut(s) 99, 147, 386, 1404, 1452
BciVI GTATCC 1 cut(s) 104
BclI TGATCA 3 cut(s) 186, 1341, 2353
BcnI CCSGG 3 cut(s) 713, 1919, 2154
BcoDI GTCTC 5 cut(s) 1046, 1164, 1254, 1482, 1972
BcuI ACTAGT 1 cut(s) 596
BfaI CTAG 7 cut(s) 597, 861, 936, 998, 1590, 2106, 2495
BfmI CTRYAG 1 cut(s) 372
BfoI RGCGCY 1 cut(s) 771
BfuAI ACCTGC 2 cut(s) 1135, 2488
BfuI GTATCC 1 cut(s) 104
BglI GCCNNNNNGGC 2 cut(s) 361, 2216
BisI GCNGC 8 cut(s) 438, 658, 690, 971, 1038, 1064, 1416, 2147
BlsI GCNGC 8 cut(s) 439, 659, 691, 972, 1039, 1065, 1417, 2148
BmcAI AGTACT 1 cut(s) 1977
Bme1390I CCNGG 8 cut(s) 99, 147, 386, 713, 1404, 1452, 1919, 2154
Bme18I GGWCC 4 cut(s) 1771, 1793, 1997, 2224
BmeT110I CYCGRG 1 cut(s) 279
BmgT120I GGNCC 5 cut(s) 1580, 1771, 1793, 1997, 2224
BmiI GGNNCC 5 cut(s) 228, 1744, 1794, 1795, 1944
BmrFI CCNGG 8 cut(s) 99, 147, 386, 713, 1404, 1452, 1919, 2154
BmsI GCATC 7 cut(s) 97, 505, 625, 1183, 1402, 1972, 2054
BplI GAGNNNNNCTC 8 cut(s) 576, 608, 947, 979, 1043, 1075, 1618, 1650
BpmI CTGGAG 2 cut(s) 276, 2409
Bpu10I CCTNAGC 1 cut(s) 1635
Bpu14I TTCGAA 1 cut(s) 1295
BpuEI CTTGAG 8 cut(s) 197, 464, 536, 839, 1080, 1146, 1688, 2262
BpuMI CCSGG 3 cut(s) 713, 1919, 2154
BsaAI YACGTR 1 cut(s) 560
BsaBI GATNNNNATC 1 cut(s) 678
BsaJI CCNNGG 7 cut(s) 97, 145, 321, 358, 711, 1403, 2153
BsaXI ACNNNNNCTCC 2 cut(s) 249, 279
Bsc4I CCNNNNNNNGG 5 cut(s) 21, 391, 820, 1451, 1789
Bse1I ACTGG 5 cut(s) 1153, 1318, 1656, 1744, 1983
Bse3DI GCAATG 2 cut(s) 135, 2226
Bse8I GATNNNNATC 1 cut(s) 678
BseBI CCWGG 5 cut(s) 99, 147, 386, 1404, 1452
BseDI CCNNGG 7 cut(s) 97, 145, 321, 358, 711, 1403, 2153
BseGI GGATG 9 cut(s) 404, 520, 535, 805, 810, 1237, 1357, 1480, 1987
BseJI GATNNNNATC 1 cut(s) 678
BseLI CCNNNNNNNGG 5 cut(s) 21, 391, 820, 1451, 1789
BseMI GCAATG 2 cut(s) 135, 2226
BseMII CTCAG 5 cut(s) 1203, 1368, 1649, 1983, 2235
BseNI ACTGG 5 cut(s) 1153, 1318, 1656, 1744, 1983
BseRI GAGGAG 2 cut(s) 595, 1691
BseXI GCAGC 6 cut(s) 449, 669, 701, 957, 1427, 2158
BseYI CCCAGC 1 cut(s) 2067
BsgI GTGCAG 1 cut(s) 864
Bsh1236I CGCG 2 cut(s) 1040, 1063
BshFI GGCC 6 cut(s) 150, 1317, 1581, 2210, 2363, 2493
BshNI GGYRCC 1 cut(s) 226
BsiHKAI GWGCWC 3 cut(s) 76, 1369, 2025
BsiHKCI CYCGRG 1 cut(s) 279
BsiSI CCGG 3 cut(s) 713, 1918, 2153
BslFI GGGAC 6 cut(s) 408, 1420, 1779, 2267, 2366, 2517
BslI CCNNNNNNNGG 5 cut(s) 21, 391, 820, 1451, 1789
BsmAI GTCTC 5 cut(s) 1046, 1164, 1254, 1482, 1972
BsmBI CGTCTC 2 cut(s) 1254, 1482
BsmFI GGGAC 6 cut(s) 408, 1420, 1779, 2267, 2366, 2517
BsmI GAATGC 3 cut(s) 256, 659, 1146
BsnI GGCC 6 cut(s) 150, 1317, 1581, 2210, 2363, 2493
BsoBI CYCGRG 1 cut(s) 279
Bsp119I TTCGAA 1 cut(s) 1295
Bsp1286I GDGCHC 3 cut(s) 76, 1369, 2025
Bsp1407I TGTACA 3 cut(s) 1486, 2203, 2451
Bsp19I CCATGG 1 cut(s) 358
BspACI CCGC 4 cut(s) 1038, 1063, 1783, 1816
BspANI GGCC 6 cut(s) 150, 1317, 1581, 2210, 2363, 2493
BspCNI CTCAG 5 cut(s) 1202, 1369, 1648, 1982, 2236
BspFNI CGCG 2 cut(s) 1040, 1063
BspHI TCATGA 1 cut(s) 2540
BspLI GGNNCC 5 cut(s) 228, 1744, 1794, 1795, 1944
BspMI ACCTGC 2 cut(s) 1135, 2488
BspPI GGATC 5 cut(s) 986, 1515, 1534, 1937, 1950
BspQI GCTCTTC 1 cut(s) 153
BspT104I TTCGAA 1 cut(s) 1295
BspT107I GGYRCC 1 cut(s) 226
BsrDI GCAATG 2 cut(s) 135, 2226
BsrGI TGTACA 3 cut(s) 1486, 2203, 2451
BsrI ACTGG 5 cut(s) 1153, 1318, 1656, 1744, 1983
BssECI CCNNGG 7 cut(s) 97, 145, 321, 358, 711, 1403, 2153
BssNAI GTATAC 1 cut(s) 850
BssT1I CCWWGG 1 cut(s) 358
Bst1107I GTATAC 1 cut(s) 850
Bst2UI CCWGG 5 cut(s) 99, 147, 386, 1404, 1452
Bst4CI ACNGT 8 cut(s) 92, 305, 1127, 1360, 1951, 2004, 2027, 2451
Bst6I CTCTTC 2 cut(s) 153, 2511
BstAPI GCANNNNNTGC 1 cut(s) 663
BstAUI TGTACA 3 cut(s) 1486, 2203, 2451
BstBAI YACGTR 1 cut(s) 560
BstBI TTCGAA 1 cut(s) 1295
BstC8I GCNNGC 4 cut(s) 23, 727, 879, 1315
BstDEI CTNAG 9 cut(s) 918, 1189, 1244, 1332, 1377, 1635, 1969, 2244, 2431
BstDSI CCRYGG 1 cut(s) 358
BstENI CCTNNNNNAGG 1 cut(s) 818
BstF5I GGATG 9 cut(s) 404, 520, 535, 805, 810, 1237, 1357, 1480, 1987
BstFNI CGCG 2 cut(s) 1040, 1063
BstH2I RGCGCY 1 cut(s) 771
BstHHI GCGC 1 cut(s) 770
BstMAI GTCTC 5 cut(s) 1046, 1164, 1254, 1482, 1972
BstMWI GCNNNNNNNGC 8 cut(s) 181, 361, 446, 455, 663, 767, 1645, 2216
BstNI CCWGG 5 cut(s) 99, 147, 386, 1404, 1452
BstNSI RCATGY 4 cut(s) 29, 522, 729, 1174
BstSCI CCNGG 8 cut(s) 97, 145, 384, 711, 1402, 1450, 1917, 2152
BstSFI CTRYAG 1 cut(s) 372
BstUI CGCG 2 cut(s) 1040, 1063
BstV1I GCAGC 6 cut(s) 449, 669, 701, 957, 1427, 2158
BstX2I RGATCY 3 cut(s) 991, 1526, 1942
BstXI CCANNNNNNTGG 1 cut(s) 84
BstYI RGATCY 3 cut(s) 991, 1526, 1942
BstZ17I GTATAC 1 cut(s) 850
BsuI GTATCC 1 cut(s) 104
BsuRI GGCC 6 cut(s) 150, 1317, 1581, 2210, 2363, 2493
BtgI CCRYGG 1 cut(s) 358
BtsCI GGATG 9 cut(s) 404, 520, 535, 805, 810, 1237, 1357, 1480, 1987
BtsI GCAGTG 1 cut(s) 456
BtsIMutI CAGTG 4 cut(s) 456, 2000, 2023, 2447
BveI ACCTGC 2 cut(s) 1135, 2488
Cac8I GCNNGC 4 cut(s) 23, 727, 879, 1315
CaiI CAGNNNCTG 2 cut(s) 692, 1409
CciI TCATGA 1 cut(s) 2540
CfoI GCGC 1 cut(s) 770
Cfr13I GGNCC 5 cut(s) 1580, 1771, 1793, 1997, 2224
CsiI ACCWGGT 1 cut(s) 1450
DdeI CTNAG 9 cut(s) 918, 1189, 1244, 1332, 1377, 1635, 1969, 2244, 2431
EaeI YGGCCR 1 cut(s) 1315
Eam1104I CTCTTC 2 cut(s) 153, 2511
EarI CTCTTC 2 cut(s) 153, 2511
Eco130I CCWWGG 1 cut(s) 358
Eco147I AGGCCT 2 cut(s) 2363, 2493
Eco32I GATATC 2 cut(s) 924, 1863
Eco47I GGWCC 4 cut(s) 1771, 1793, 1997, 2224
Eco57I CTGAAG 3 cut(s) 240, 1206, 1698
Eco72I CACGTG 1 cut(s) 560
Eco88I CYCGRG 1 cut(s) 279
EcoNI CCTNNNNNAGG 1 cut(s) 818
EcoO109I RGGNCCY 1 cut(s) 1793
EcoRII CCWGG 5 cut(s) 97, 145, 384, 1402, 1450
EcoRV GATATC 2 cut(s) 924, 1863
EcoT14I CCWWGG 1 cut(s) 358
EcoT22I ATGCAT 3 cut(s) 66, 520, 727
ErhI CCWWGG 1 cut(s) 358
Esp3I CGTCTC 2 cut(s) 1254, 1482
FalI AAGNNNNNCTT 2 cut(s) 2414, 2446
FaqI GGGAC 6 cut(s) 408, 1420, 1779, 2267, 2366, 2517
FauNDI CATATG 2 cut(s) 662, 1177
FbaI TGATCA 3 cut(s) 186, 1341, 2353
FblI GTMKAC 1 cut(s) 849
Fnu4HI GCNGC 8 cut(s) 438, 658, 690, 971, 1038, 1064, 1416, 2147
FokI GGATG 9 cut(s) 391, 527, 542, 797, 812, 1244, 1364, 1467, 1994
Fsp4HI GCNGC 8 cut(s) 438, 658, 690, 971, 1038, 1064, 1416, 2147
FspBI CTAG 7 cut(s) 597, 861, 936, 998, 1590, 2106, 2495
GlaI GCGC 1 cut(s) 769
GluI GCNGC 8 cut(s) 438, 658, 690, 971, 1038, 1064, 1416, 2147
GsaI CCCAGC 1 cut(s) 2071
GsuI CTGGAG 2 cut(s) 276, 2409
HaeII RGCGCY 1 cut(s) 771
HaeIII GGCC 6 cut(s) 150, 1317, 1581, 2210, 2363, 2493
HapII CCGG 3 cut(s) 713, 1918, 2153
HhaI GCGC 1 cut(s) 770
Hin6I GCGC 1 cut(s) 768
HinP1I GCGC 1 cut(s) 768
HincII GTYRAC 1 cut(s) 1123
HindII GTYRAC 1 cut(s) 1123
HpaI GTTAAC 1 cut(s) 1123
HpaII CCGG 3 cut(s) 713, 1918, 2153
HphI GGTGA 7 cut(s) 29, 192, 1061, 1439, 1526, 2089, 2184
Hpy166II GTNNAC 6 cut(s) 850, 1123, 1489, 1517, 1997, 2187
Hpy188I TCNGA 9 cut(s) 220, 249, 1192, 1288, 1378, 1972, 2245, 2368, 2408
Hpy8I GTNNAC 6 cut(s) 850, 1123, 1489, 1517, 1997, 2187
HpyAV CCTTC 8 cut(s) 215, 505, 824, 824, 1250, 1783, 2144, 2416
HpyCH4III ACNGT 8 cut(s) 92, 305, 1127, 1360, 1951, 2004, 2027, 2451
HpyCH4IV ACGT 1 cut(s) 559
HpyF10VI GCNNNNNNNGC 8 cut(s) 181, 361, 446, 455, 663, 767, 1645, 2216
HpyF3I CTNAG 9 cut(s) 918, 1189, 1244, 1332, 1377, 1635, 1969, 2244, 2431
HpySE526I ACGT 1 cut(s) 559
HspAI GCGC 1 cut(s) 768
KflI GGGWCCC 1 cut(s) 1793
KpnI GGTACC 1 cut(s) 230
Ksp22I TGATCA 3 cut(s) 186, 1341, 2353
KspAI GTTAAC 1 cut(s) 1123
LguI GCTCTTC 1 cut(s) 153
LmnI GCTCC 7 cut(s) 79, 343, 388, 571, 765, 1372, 2020
Lsp1109I GCAGC 6 cut(s) 449, 669, 701, 957, 1427, 2158
LweI GCATC 7 cut(s) 97, 505, 625, 1183, 1402, 1972, 2054
MabI ACCWGGT 1 cut(s) 1450
MaeI CTAG 7 cut(s) 597, 861, 936, 998, 1590, 2106, 2495
MaeII ACGT 1 cut(s) 559
MaeIII GTNAC 5 cut(s) 1183, 1628, 1880, 1958, 2497
MflI RGATCY 3 cut(s) 991, 1526, 1942
MhlI GDGCHC 3 cut(s) 76, 1369, 2025
MlsI TGGCCA 1 cut(s) 1317
MluNI TGGCCA 1 cut(s) 1317
MlyI GAGTC 6 cut(s) 858, 1030, 2097, 2167, 2249, 2400
MmeI TCCRAC 3 cut(s) 1302, 1970, 2391
Mox20I TGGCCA 1 cut(s) 1317
Mph1103I ATGCAT 3 cut(s) 66, 520, 727
MroXI GAANNNNTTC 2 cut(s) 1089, 2520
MscI TGGCCA 1 cut(s) 1317
MseI TTAA 8 cut(s) 269, 312, 731, 1101, 1122, 2441, 2462, 2511
MslI CAYNNNNRTG 3 cut(s) 69, 1648, 1932
Msp20I TGGCCA 1 cut(s) 1317
MspA1I CMGCKG 4 cut(s) 692, 970, 1118, 2057
MspI CCGG 3 cut(s) 713, 1918, 2153
MspR9I CCNGG 8 cut(s) 99, 147, 386, 713, 1404, 1452, 1919, 2154
Mva1269I GAATGC 3 cut(s) 256, 659, 1146
MvaI CCWGG 5 cut(s) 99, 147, 386, 1404, 1452
MvnI CGCG 2 cut(s) 1040, 1063
MwoI GCNNNNNNNGC 8 cut(s) 181, 361, 446, 455, 663, 767, 1645, 2216
NciI CCSGG 3 cut(s) 713, 1919, 2154
NcoI CCATGG 1 cut(s) 358
NdeI CATATG 2 cut(s) 662, 1177
NlaIV GGNNCC 5 cut(s) 228, 1744, 1794, 1795, 1944
NmeAIII GCCGAG 1 cut(s) 346
NmuCI GTSAC 4 cut(s) 1183, 1628, 1958, 2497
NsiI ATGCAT 3 cut(s) 66, 520, 727
NspI RCATGY 4 cut(s) 29, 522, 729, 1174
NspV TTCGAA 1 cut(s) 1295
OliI CACNNNNGTG 1 cut(s) 1932
PaeI GCATGC 1 cut(s) 729
PaeR7I CTCGAG 1 cut(s) 279
PagI TCATGA 1 cut(s) 2540
PceI AGGCCT 2 cut(s) 2363, 2493
PciI ACATGT 1 cut(s) 1170
PciSI GCTCTTC 1 cut(s) 153
PctI GAATGC 3 cut(s) 256, 659, 1146
PdmI GAANNNNTTC 2 cut(s) 1089, 2520
PfeI GAWTC 6 cut(s) 1112, 1469, 1821, 2287, 2470, 2521
PflMI CCANNNNNTGG 1 cut(s) 1451
PkrI GCNGC 8 cut(s) 439, 659, 691, 972, 1039, 1065, 1417, 2148
PleI GAGTC 6 cut(s) 858, 1029, 2096, 2166, 2249, 2399
PmaCI CACGTG 1 cut(s) 560
PmlI CACGTG 1 cut(s) 560
PpsI GAGTC 6 cut(s) 858, 1029, 2096, 2166, 2249, 2399
Ppu21I YACGTR 1 cut(s) 560
PpuMI RGGWCCY 1 cut(s) 1793
PscI ACATGT 1 cut(s) 1170
Psp5II RGGWCCY 1 cut(s) 1793
Psp6I CCWGG 5 cut(s) 97, 145, 384, 1402, 1450
PspCI CACGTG 1 cut(s) 560
PspFI CCCAGC 1 cut(s) 2067
PspGI CCWGG 5 cut(s) 97, 145, 384, 1402, 1450
PspN4I GGNNCC 5 cut(s) 228, 1744, 1794, 1795, 1944
PspPI GGNCC 5 cut(s) 1580, 1771, 1793, 1997, 2224
PspPPI RGGWCCY 1 cut(s) 1793
PspXI VCTCGAGB 1 cut(s) 279
PsrI GAACNNNNNNTAC 2 cut(s) 459, 491
PstNI CAGNNNCTG 2 cut(s) 692, 1409
PsuI RGATCY 3 cut(s) 991, 1526, 1942
PvuII CAGCTG 4 cut(s) 692, 970, 1118, 2057
RseI CAYNNNNRTG 3 cut(s) 69, 1648, 1932
SapI GCTCTTC 1 cut(s) 153
SaqAI TTAA 8 cut(s) 269, 312, 731, 1101, 1122, 2441, 2462, 2511
SatI GCNGC 8 cut(s) 438, 658, 690, 971, 1038, 1064, 1416, 2147
Sau96I GGNCC 5 cut(s) 1580, 1771, 1793, 1997, 2224
ScaI AGTACT 1 cut(s) 1977
SchI GAGTC 6 cut(s) 858, 1030, 2097, 2167, 2249, 2400
ScrFI CCNGG 8 cut(s) 99, 147, 386, 713, 1404, 1452, 1919, 2154
SduI GDGCHC 3 cut(s) 76, 1369, 2025
SexAI ACCWGGT 1 cut(s) 1450
SfaNI GCATC 7 cut(s) 97, 505, 625, 1183, 1402, 1972, 2054
SfcI CTRYAG 1 cut(s) 372
Sfr274I CTCGAG 1 cut(s) 279
SfuI TTCGAA 1 cut(s) 1295
SinI GGWCC 4 cut(s) 1771, 1793, 1997, 2224
SlaI CTCGAG 1 cut(s) 279
SmiMI CAYNNNNRTG 3 cut(s) 69, 1648, 1932
SmlI CTYRAG 9 cut(s) 212, 279, 479, 551, 818, 1095, 1161, 1667, 2277
SmoI CTYRAG 9 cut(s) 212, 279, 479, 551, 818, 1095, 1161, 1667, 2277
SpeI ACTAGT 1 cut(s) 596
SphI GCATGC 1 cut(s) 729
SseBI AGGCCT 2 cut(s) 2363, 2493
SsiI CCGC 4 cut(s) 1038, 1063, 1783, 1816
SspMI CTAG 7 cut(s) 597, 861, 936, 998, 1590, 2106, 2495
StuI AGGCCT 2 cut(s) 2363, 2493
StyD4I CCNGG 8 cut(s) 97, 145, 384, 711, 1402, 1450, 1917, 2152
StyI CCWWGG 1 cut(s) 358
TaaI ACNGT 8 cut(s) 92, 305, 1127, 1360, 1951, 2004, 2027, 2451
TaiI ACGT 1 cut(s) 562
TaqI TCGA 5 cut(s) 280, 867, 1295, 2394, 2488
TaqII GACCGA 2 cut(s) 1788, 2241
TatI WGTACW 6 cut(s) 45, 1486, 1975, 2203, 2281, 2451
TauI GCSGC 2 cut(s) 1040, 1066
TfiI GAWTC 6 cut(s) 1112, 1469, 1821, 2287, 2470, 2521
Tru1I TTAA 8 cut(s) 269, 312, 731, 1101, 1122, 2441, 2462, 2511
Tru9I TTAA 8 cut(s) 269, 312, 731, 1101, 1122, 2441, 2462, 2511
TscAI CASTG 4 cut(s) 456, 2007, 2030, 2454
TseFI GTSAC 4 cut(s) 1183, 1628, 1958, 2497
TseI GCWGC 6 cut(s) 437, 657, 689, 970, 1415, 2146
Tsp45I GTSAC 4 cut(s) 1183, 1628, 1958, 2497
TspRI CASTG 4 cut(s) 456, 2007, 2030, 2454
Van91I CCANNNNNTGG 1 cut(s) 1451
VpaK11BI GGWCC 4 cut(s) 1771, 1793, 1997, 2224
XagI CCTNNNNNAGG 1 cut(s) 818
XapI RAATTY 4 cut(s) 116, 587, 746, 1302
XbaI TCTAGA 1 cut(s) 935
XceI RCATGY 4 cut(s) 29, 522, 729, 1174
XcmI CCANNNNNNNNNTGG 1 cut(s) 2218
XhoI CTCGAG 1 cut(s) 279
XmiI GTMKAC 1 cut(s) 849
XmnI GAANNNNTTC 2 cut(s) 1089, 2520
XspI CTAG 7 cut(s) 597, 861, 936, 998, 1590, 2106, 2495
ZrmI AGTACT 1 cut(s) 1977
Zsp2I ATGCAT 3 cut(s) 66, 520, 727
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.