MD12G1246900.v1.1

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002633
Physical Location & Seq
Reverse (-)
31721067 .. 31728936
7870 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1246900.v1.1.491

Sequence Viewer

Length: 3711 bp
ATGGGGACAGAACTCATGAGAGTTTGCATAAAAGATGATAACGAGGAGTTTCCTTCAGTTCCACCCGGATTTGAGTCATTTGCACCTTTCAGCTTAAAAAGGATAAATGAAAGTGAGAAAAAAGATATTGAAAACAGAATAAGTTGCTCAGTGACTGCTAGTCCTTCCAATTCACAGTCAGTTCAGATGGAAACTGACGTTGACAATGGCGAGGTTGCAAAGAGGTCTGTTCGACGCAGACAATGCATAAACCATGGGCGAAATAACAAGTCAGAGGATGAGTCTGATTCAGACCGACTTGAACACAATTGTCCCCCAAGATCTGTTCTTCCCAAGGGGGTTGTACGCGGATGTCCACAATGTAGTAATTGCCAAAAGGTCTCTGCAAGATGGCATCCACTGGAGGGTCAAAGGCGGGACCTTCAGAACGCTCCTGTATTCAGACCTACTGAGGAGGAGTTCAAAGACACGTTGAAATATATAGCAAGCATACGCGCTAAAGCCGAACCATATGGTATTTGTCGCATTGTTCCTCCCTCTTCTTGGAGACCTCCCTGTCCACTCAAGGAAAAACCTATTTGGAGTGCCTCTAAATTTTCTACTCGAGTTCAAAGAGTCGATAAACTGCAAAATCGAGATTCAATGAGAAAGGTTCCTAAAAGCCATAGTCATATGAGGAAGAAAAGAAGAAGATGCACACGGATGGGGGCTGATCGCCAAAGTGGTGGCAGAGGATCTGAGGATGATGGAGGTTGTGAGGCTGAACGGTTTGGGTTTGAACCTGGACCAGAGTTTACTCTAGAAGCATTTGAAAGATATGCTGATGATTTCAAGGCTCAATACTTTGGCAAGAACGAACATGTTCCTAGTATAGGAGGTAACTTTGCTAAACTTAAAGAGTGCTGGGAGCCATCTGTAGGCAGTATTGAGGGTGAATATTGGCGGATGGTAGAGAGACCTACTGAAGAAATTGAGGTTCTATATGGTGCTGATTTGGAAACTGGAGTTTTTGGCAGTGGCTTTCCCAAGAAATCCAGTAAGGATGCTTTTCCTTCAGAAGAGCAGTACATAAATTCAGGGTGGAACTTGAACAACTTTCCCAGACTTACTGGATCTGTTCTTTCTTATGAAAGCAGTGATATATCTGGTGTTCTGGTGCCTTGGTTATACATAGGGATGTGCTTTTCTTCCTTTTGCTGGCATGTTGAAGATCACCACTTGTACTCTTTAAATTATATGCATTGGGGTGCTCCAAAGTTGTGGTACGGTATCCCTGGTAAAGATGCCTGTAAATTTGAAGAAGCAATGAGAAAGCACCTCCCTGGCCTATTTGAAGAGCAACCTGACTTGCTTCATAAGCTGGTCACACAGCTTTCACCCTCCATTCTGAAATCTGAGGGGGTACCTGTGTATAGATGTTGTCAGAATGCTGGAGAGTTTGTTTTGACCTTTCCTCGAGCATACCACTCAGGGTTCAACTGTGGTTTTAATTGTGCCGAGGCTGTGAATGTAGCTCCTGTTGATTGGTTGCCCCATGGGCAGATCGCTATAGAGCTATACCAGGAGCAGGGACGAAAGACATCCATTTCTCATGATAAATTGTTGCTTGGGGCAGCAAGGGAAGCGGTGAAAGCACATTGGGAACTCAATTTACTGAAGAAGAATACTCCAGATAACTTAAGGTGGAAGGATGTATGTGGAAAGGATGGGACCTTAGCAAAAACACTCAAGGCACGTGTCGAGATAGAGCGTGCAAGGAGAGAATTTCTCACTACTTCTTCACAGGCATTGAAGATGGAAAACAATTTTGATGCCACCAATGAGAGGGAATGCAGCATATGCTTTTTTGATCTGCATCTGTCAGCAGCTGGTTGTCATCATTGTTCCCCAGATAGATATGCATGCTTAAATCATGCAAAGAAATTCTGCTCATGTGCTTGGAGCGCCAAGTTTTTCCTCTTTCGTTATGATATGGATGAACTAAACATTCTTCTTGAGGCATTGGAAGGAAAGTTGAGTGCAGTATACAGATGGGCTAGACTCGATCTTGGTTTGGCTCTGAGTTCTTATATTTCCAAAGACAATAAAGTTGGTAAGATATCTTATTCCTCCAAAAGTGATATACTTACAGAGGTTAGATCACAGCTGCAAAGCACACACTTCAAGGATCCTCCAGGAACAGAAATCTCTAAAGAGAGTCCTATGAGTTCAACCGAGATCATTGTTGAGACTTCCTCGCAACAGAAGAGGAAAGCATTAGAAACTTTTGCTCAAGTTAAAAGAGAAGAATCAGGTCTTAACAGTTCCAAGTCAAGAATGCAGGTTTGCCAGTTGTCTCAAGAGGACACGTCATATGCTGTGACCTCGGATGCAAAGGTATCTGGGAGAAATATGGCTTCAGTTGTGGATGTTATTCTTCTTAGTGACGATGAAGGAGACGCCGACCCAACGAAGCCACTTTCAGATAGTTTGAAAGAGATATCTTCTGCTAACCAGTTAGAACTTTCTAAGAGATTGGTCAGTTCGGATGGAATAGCGAGCGCTCCTAATTTTGATAGAGAACCAATCTTGAATACACCAGGGACTGATGCAGCAGTGATGGGTGAAAAAGTTATCAGTCCGTTACCTGGTGGAGAAAAAAAGGATTCATCATCTCACCCTGTACACGTGAAAATTGAACAGGATCGTGGTGAACAATTAGGATCTAATCCACCAAACCTCTCTTTCAAAATAGTTTCTGTTAAAGCAGATTGTGGCCCAAACACTGGTGCTATCATTGAACACAAAGTTGCTATTTCAAGGAGTGACCCACAGCCATGTGCCAGTGTAAAACTTGAGAATGAGGACAGACATGAAAAGATGGGAAGAATTGCCGATACAACTTTAGCAGACAATGTACGAATGACAACTGGAACTTCGTCGTCCAGCCAAAACAATTTGGACCGATATTACCGCCAAAGGGGTCCCCGAATAGCGAAGGTGGTGCGGAGAATCTCCTGCGTTGTTGAGCCTCTGGAAATTGGAGTCGTGCTATCTGGAAAGTCATGGTGTAACACCCAAGCCATTTTTCCAAAAGGATTTAGGAGCCGGGTTAGGCACATGAGTGTTCTGGATCCAACTGTTATGTGTCATTATGTCTCAGAAGTACTGGATGCCGGACAGGGTGGACCACTGTTCAAGGTTTCTTTGGAGCATTGTCCAAGTGAGGTTTTCATACATAATTCAGCTGACAGATGCTGGGAAATGGTGAGAGAGAGAGTCAATCAAGAAATCACGAGGCAACATAAATTGGGAAAAATGAACCTTCCTCCTTTGCAGCCACCGGGGAGTCTCGATGGCTTTGAAATGTTTGGGTTTACTTCACCAGCTATTGTACAGGCCATAGAGGCAATGGATCGAAATCGTGTCTGTTCTGAGTACTGGGACTCTCGTCCCTACTCCCGACTTCAAGTACAGATTCCACGGAACTCTCAATCCAAAGAAAGTAGTGAAAATTGCAATTCGATGTCAAAGGAAAAAAGTGATCAGGCGGCCTCCGACAATGATCTCGTCCCTGCTGGAGTTGATACAACACTCAGAGGCCTATTCAAGAAGGCTAACCTAGACGAATTGAACTCACTGTACAGCATTTTAAGTGACAATCGGCAACCTGCTGGTCGAGGCCTGGTCACACGACTTCTTTACGAAGAGATTCACAATCGTCCCACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000976 GO:0001067 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0003824 GO:0005488 GO:0005506 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006325 GO:0006355 GO:0006464 GO:0006479 GO:0006482 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008198 GO:0008213 GO:0008214 GO:0008276 GO:0009314 GO:0009416 GO:0009628 GO:0009648 GO:0009791 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009910 GO:0009987 GO:0010216 GO:0010228 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0016577 GO:0016740 GO:0016741 GO:0019219 GO:0019222 GO:0019538 GO:0022414 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0032451 GO:0032452 GO:0032453 GO:0032501 GO:0032502 GO:0034641 GO:0034720 GO:0036211 GO:0040008 GO:0040009 GO:0040010 GO:0040029 GO:0042054 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045814 GO:0045892 GO:0045927 GO:0045934 GO:0046483 GO:0046872 GO:0046914 GO:0048518 GO:0048519 GO:0048523 GO:0048573 GO:0048579 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048586 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051172 GO:0051239 GO:0051241 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0140096 GO:0140110 GO:1901360 GO:1901363 GO:1901564 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000026 GO:2000028 GO:2000112 GO:2000113 GO:2000241 GO:2000242 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FYRC FYRN JmjC JmjN zf-C5HC2

Protein Analysis

1237

Amino Acids

138.65

Weight (kDa)

7.14

Isoelectric Point (pI)

51.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JmjN PF02375 145 - 178 1.1e-17 jmjN domain
JmjC PF02373 388 - 504 7.1e-46 JmjC domain, hydroxylase
zf-C5HC2 PF02928 611 - 663 5.4e-16 C5HC2 zinc finger
FYRN PF05964 1006 - 1051 1.2e-08 F/Y-rich N-terminus
FYRC PF05965 1056 - 1140 6.5e-23 F/Y rich C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 555
Acc36I ACCTGC 2 cut(s) 2311, 3658
Acc65I GGTACC 1 cut(s) 1402
AccB1I GGYRCC 2 cut(s) 1156, 1402
AccB7I CCANNNNNTGG 1 cut(s) 2765
AccI GTMKAC 1 cut(s) 2025
AccII CGCG 2 cut(s) 348, 495
AciI CCGC 7 cut(s) 348, 415, 943, 1625, 2953, 2986, 3530
AclWI GGATC 9 cut(s) 742, 1120, 2162, 2175, 2691, 2710, 3107, 3120, 3402
AcsI RAATTY 5 cut(s) 593, 1072, 1292, 1763, 1922
AcuI CTGAAG 6 cut(s) 39, 407, 984, 1038, 1676, 2382
AcvI CACGTG 2 cut(s) 1736, 2668
AcyI GRCGYC 1 cut(s) 2439
AfeI AGCGCT 1 cut(s) 2542
AflII CTTAAG 1 cut(s) 1678
AflIII ACRYGT 5 cut(s) 468, 859, 1735, 2346, 2665
AhdI GACNNNNNGTC 1 cut(s) 159
AjiI CACGTC 1 cut(s) 2349
AjnI CCWGG 8 cut(s) 781, 1273, 1321, 1560, 2173, 2578, 2626, 3663
AjuI GAANNNNNNNTTGG 2 cut(s) 3051, 3083
AleI CACNNNNGTG 1 cut(s) 3102
AloI GAACNNNNNNTCC 2 cut(s) 3458, 3490
AluBI AGCT 9 cut(s) 93, 1360, 1372, 1514, 1555, 1868, 2146, 3227, 3368
AluI AGCT 9 cut(s) 93, 1360, 1372, 1514, 1555, 1868, 2146, 3227, 3368
Alw21I GWGCWC 1 cut(s) 1252
Alw26I GTCTC 8 cut(s) 385, 541, 949, 2222, 2340, 2430, 3142, 3335
AlwI GGATC 9 cut(s) 742, 1120, 2162, 2175, 2691, 2710, 3107, 3120, 3402
AlwNI CAGNNNCTG 4 cut(s) 155, 1868, 2585, 3237
Ama87I CYCGRG 2 cut(s) 603, 1455
Aor51HI AGCGCT 1 cut(s) 2542
AoxI GGCC 6 cut(s) 1324, 2755, 3378, 3531, 3580, 3661
ApeKI GCWGC 6 cut(s) 1613, 1833, 1865, 2146, 2591, 3316
ApoI RAATTY 5 cut(s) 593, 1072, 1292, 1763, 1922
Asp700I GAANNNNTTC 2 cut(s) 861, 3690
Asp718I GGTACC 1 cut(s) 1402
AspLEI GCGC 3 cut(s) 497, 1946, 2543
AspS9I GGNCC 7 cut(s) 418, 785, 1710, 2756, 2941, 2963, 3167
AsuC2I CCSGG 3 cut(s) 66, 3089, 3324
AsuHPI GGTGA 9 cut(s) 944, 1205, 1368, 1639, 2615, 2648, 2702, 3259, 3354
AvaI CYCGRG 2 cut(s) 603, 1455
AvaII GGWCC 6 cut(s) 418, 785, 1710, 2941, 2963, 3167
BaeI ACNNNNGTAYC 2 cut(s) 1394, 1427
BamHI GGATCC 2 cut(s) 2167, 3112
BanI GGYRCC 2 cut(s) 1156, 1402
BauI CACGAG 1 cut(s) 3274
BbrPI CACGTG 2 cut(s) 1736, 2668
Bbv12I GWGCWC 1 cut(s) 1252
BbvI GCAGC 6 cut(s) 1625, 1845, 1877, 2133, 2603, 3328
BcgI CGANNNNNNTGC 2 cut(s) 2965, 2999
BciT130I CCWGG 8 cut(s) 783, 1275, 1323, 1562, 2175, 2580, 2628, 3665
BciVI GTATCC 1 cut(s) 1280
BclI TGATCA 1 cut(s) 3523
BcnI CCSGG 3 cut(s) 66, 3089, 3324
BcoDI GTCTC 8 cut(s) 385, 541, 949, 2222, 2340, 2430, 3142, 3335
BfaI CTAG 5 cut(s) 159, 800, 867, 2037, 3602
BfmI CTRYAG 2 cut(s) 915, 1548
BfoI RGCGCY 2 cut(s) 1947, 2544
BfrI CTTAAG 1 cut(s) 1678
BfuAI ACCTGC 2 cut(s) 2311, 3658
BfuI GTATCC 1 cut(s) 1280
BglI GCCNNNNNGGC 2 cut(s) 1537, 3386
BglII AGATCT 1 cut(s) 320
BisI GCNGC 7 cut(s) 1614, 1834, 1866, 2147, 2592, 3317, 3531
BlsI GCNGC 7 cut(s) 1615, 1835, 1867, 2148, 2593, 3318, 3532
BmcAI AGTACT 2 cut(s) 3147, 3419
Bme18I GGWCC 6 cut(s) 418, 785, 1710, 2941, 2963, 3167
BmeRI GACNNNNNGTC 1 cut(s) 159
BmeT110I CYCGRG 2 cut(s) 603, 1455
BmgBI CACGTC 1 cut(s) 2349
BmgT120I GGNCC 7 cut(s) 418, 785, 1710, 2756, 2941, 2963, 3167
BmrI ACTGGG 1 cut(s) 3430
BmuI ACTGGG 1 cut(s) 3430
BoxI GACNNNNGTC 1 cut(s) 3429
BplI GAGNNNNNCTC 4 cut(s) 1752, 1784, 2219, 2251
BpmI CTGGAG 6 cut(s) 422, 1023, 1452, 1653, 2157, 3579
Bpu10I CCTNAGC 1 cut(s) 1714
BpuEI CTTGAG 6 cut(s) 548, 1712, 2015, 2256, 2322, 2855
BpuMI CCSGG 3 cut(s) 66, 3089, 3324
BsaAI YACGTR 2 cut(s) 1736, 2668
BsaBI GATNNNNATC 2 cut(s) 1854, 3399
BsaHI GRCGYC 1 cut(s) 2439
BsaI GGTCTC 3 cut(s) 385, 541, 949
BsaXI ACNNNNNCTCC 2 cut(s) 1425, 1455
Bse3DI GCAATG 2 cut(s) 1311, 3396
Bse8I GATNNNNATC 2 cut(s) 1854, 3399
BseBI CCWGG 8 cut(s) 783, 1275, 1323, 1562, 2175, 2580, 2628, 3665
BseJI GATNNNNATC 2 cut(s) 1854, 3399
BseMI GCAATG 2 cut(s) 1311, 3396
BseMII CTCAG 9 cut(s) 162, 441, 729, 1386, 1482, 2051, 3153, 3405, 3589
BseRI GAGGAG 3 cut(s) 59, 467, 470
BseXI GCAGC 6 cut(s) 1625, 1845, 1877, 2133, 2603, 3328
BseYI CCCAGC 2 cut(s) 903, 3237
BsgI GTGCAG 1 cut(s) 2040
Bsh1236I CGCG 2 cut(s) 348, 495
BshFI GGCC 6 cut(s) 1326, 2757, 3380, 3533, 3582, 3663
BshNI GGYRCC 2 cut(s) 1156, 1402
BsiHKAI GWGCWC 1 cut(s) 1252
BsiHKCI CYCGRG 2 cut(s) 603, 1455
BsiSI CCGG 4 cut(s) 66, 3088, 3156, 3323
BsmAI GTCTC 8 cut(s) 385, 541, 949, 2222, 2340, 2430, 3142, 3335
BsmBI CGTCTC 1 cut(s) 2430
BsmI GAATGC 3 cut(s) 1432, 1835, 2322
BsnI GGCC 6 cut(s) 1326, 2757, 3380, 3533, 3582, 3663
Bso31I GGTCTC 3 cut(s) 385, 541, 949
BsoBI CYCGRG 2 cut(s) 603, 1455
Bsp1286I GDGCHC 1 cut(s) 1252
Bsp1407I TGTACA 3 cut(s) 2662, 3373, 3621
Bsp19I CCATGG 2 cut(s) 253, 1534
BspACI CCGC 7 cut(s) 348, 415, 943, 1625, 2953, 2986, 3530
BspANI GGCC 6 cut(s) 1326, 2757, 3380, 3533, 3582, 3663
BspCNI CTCAG 9 cut(s) 161, 442, 730, 1387, 1481, 2052, 3152, 3406, 3588
BspFNI CGCG 2 cut(s) 348, 495
BspHI TCATGA 2 cut(s) 15, 1591
BspMI ACCTGC 2 cut(s) 2311, 3658
BspPI GGATC 9 cut(s) 742, 1120, 2162, 2175, 2691, 2710, 3107, 3120, 3402
BspQI GCTCTTC 2 cut(s) 1053, 1329
BspT107I GGYRCC 2 cut(s) 1156, 1402
BspTI CTTAAG 1 cut(s) 1678
BspTNI GGTCTC 3 cut(s) 385, 541, 949
BsrDI GCAATG 2 cut(s) 1311, 3396
BsrGI TGTACA 3 cut(s) 2662, 3373, 3621
BssNAI GTATAC 1 cut(s) 2026
BssNI GRCGYC 1 cut(s) 2439
BssSI CACGAG 1 cut(s) 3274
BssT1I CCWWGG 4 cut(s) 253, 333, 1160, 1534
Bst1107I GTATAC 1 cut(s) 2026
Bst2BI CACGAG 1 cut(s) 3274
Bst2UI CCWGG 8 cut(s) 783, 1275, 1323, 1562, 2175, 2580, 2628, 3665
Bst4CI ACNGT 8 cut(s) 177, 768, 1268, 1481, 2303, 3121, 3174, 3621
Bst6I CTCTTC 5 cut(s) 544, 1053, 1329, 2240, 3681
BstACI GRCGYC 1 cut(s) 2439
BstAFI CTTAAG 1 cut(s) 1678
BstAPI GCANNNNNTGC 2 cut(s) 243, 1839
BstAUI TGTACA 3 cut(s) 2662, 3373, 3621
BstBAI YACGTR 2 cut(s) 1736, 2668
BstC8I GCNNGC 5 cut(s) 487, 1199, 1752, 1903, 2539
BstDSI CCRYGG 3 cut(s) 253, 1534, 3461
BstENI CCTNNNNNAGG 1 cut(s) 870
BstFNI CGCG 2 cut(s) 348, 495
BstH2I RGCGCY 2 cut(s) 1947, 2544
BstHHI GCGC 3 cut(s) 497, 1946, 2543
BstMAI GTCTC 8 cut(s) 385, 541, 949, 2222, 2340, 2430, 3142, 3335
BstMWI GCNNNNNNNGC 8 cut(s) 243, 1357, 1537, 1622, 1631, 1839, 1943, 3386
BstNI CCWGG 8 cut(s) 783, 1275, 1323, 1562, 2175, 2580, 2628, 3665
BstNSI RCATGY 3 cut(s) 863, 1205, 1905
BstPAI GACNNNNGTC 1 cut(s) 3429
BstSFI CTRYAG 2 cut(s) 915, 1548
BstUI CGCG 2 cut(s) 348, 495
BstV1I GCAGC 6 cut(s) 1625, 1845, 1877, 2133, 2603, 3328
BstX2I RGATCY 6 cut(s) 320, 734, 1112, 2167, 2702, 3112
BstXI CCANNNNNNTGG 2 cut(s) 725, 1260
BstYI RGATCY 6 cut(s) 320, 734, 1112, 2167, 2702, 3112
BstZ17I GTATAC 1 cut(s) 2026
BsuI GTATCC 1 cut(s) 1280
BsuRI GGCC 6 cut(s) 1326, 2757, 3380, 3533, 3582, 3663
BtgI CCRYGG 3 cut(s) 253, 1534, 3461
BtrI CACGTC 1 cut(s) 2349
BtsI GCAGTG 3 cut(s) 1021, 1141, 2601
BtsIMutI CAGTG 9 cut(s) 156, 398, 1021, 1141, 2601, 2763, 2830, 3170, 3617
BveI ACCTGC 2 cut(s) 2311, 3658
Cac8I GCNNGC 5 cut(s) 487, 1199, 1752, 1903, 2539
CaiI CAGNNNCTG 4 cut(s) 155, 1868, 2585, 3237
CciI TCATGA 2 cut(s) 15, 1591
CfoI GCGC 3 cut(s) 497, 1946, 2543
Cfr13I GGNCC 7 cut(s) 418, 785, 1710, 2756, 2941, 2963, 3167
CseI GACGC 2 cut(s) 243, 2447
CsiI ACCWGGT 1 cut(s) 2626
DraI TTTAAA 1 cut(s) 1230
DrdI GACNNNNNNGTC 1 cut(s) 555
DriI GACNNNNNGTC 1 cut(s) 159
DseDI GACNNNNNNGTC 1 cut(s) 555
Eam1104I CTCTTC 5 cut(s) 544, 1053, 1329, 2240, 3681
Eam1105I GACNNNNNGTC 1 cut(s) 159
EarI CTCTTC 5 cut(s) 544, 1053, 1329, 2240, 3681
EciI GGCGGA 1 cut(s) 958
Eco130I CCWWGG 4 cut(s) 253, 333, 1160, 1534
Eco147I AGGCCT 2 cut(s) 3582, 3663
Eco31I GGTCTC 3 cut(s) 385, 541, 949
Eco32I GATATC 2 cut(s) 2100, 2481
Eco47I GGWCC 6 cut(s) 418, 785, 1710, 2941, 2963, 3167
Eco47III AGCGCT 1 cut(s) 2542
Eco57I CTGAAG 6 cut(s) 39, 407, 984, 1038, 1676, 2382
Eco72I CACGTG 2 cut(s) 1736, 2668
Eco88I CYCGRG 2 cut(s) 603, 1455
EcoNI CCTNNNNNAGG 1 cut(s) 870
EcoO109I RGGNCCY 3 cut(s) 418, 1710, 2963
EcoRII CCWGG 8 cut(s) 781, 1273, 1321, 1560, 2173, 2578, 2626, 3663
EcoRV GATATC 2 cut(s) 2100, 2481
EcoT14I CCWWGG 4 cut(s) 253, 333, 1160, 1534
EcoT22I ATGCAT 3 cut(s) 248, 1242, 1903
ErhI CCWWGG 4 cut(s) 253, 333, 1160, 1534
Esp3I CGTCTC 1 cut(s) 2430
FauI CCCGC 1 cut(s) 408
FauNDI CATATG 4 cut(s) 511, 672, 1838, 2353
FbaI TGATCA 1 cut(s) 3523
FblI GTMKAC 1 cut(s) 2025
Fnu4HI GCNGC 7 cut(s) 1614, 1834, 1866, 2147, 2592, 3317, 3531
Fsp4HI GCNGC 7 cut(s) 1614, 1834, 1866, 2147, 2592, 3317, 3531
FspBI CTAG 5 cut(s) 159, 800, 867, 2037, 3602
GlaI GCGC 3 cut(s) 496, 1945, 2542
GluI GCNGC 7 cut(s) 1614, 1834, 1866, 2147, 2592, 3317, 3531
GsaI CCCAGC 2 cut(s) 907, 3241
GsuI CTGGAG 6 cut(s) 422, 1023, 1452, 1653, 2157, 3579
HaeII RGCGCY 2 cut(s) 1947, 2544
HaeIII GGCC 6 cut(s) 1326, 2757, 3380, 3533, 3582, 3663
HapII CCGG 4 cut(s) 66, 3088, 3156, 3323
HgaI GACGC 2 cut(s) 243, 2447
HhaI GCGC 3 cut(s) 497, 1946, 2543
Hin1I GRCGYC 1 cut(s) 2439
Hin6I GCGC 3 cut(s) 495, 1944, 2541
HinP1I GCGC 3 cut(s) 495, 1944, 2541
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HpaII CCGG 4 cut(s) 66, 3088, 3156, 3323
HphI GGTGA 9 cut(s) 944, 1205, 1368, 1639, 2615, 2648, 2702, 3259, 3354
Hpy166II GTNNAC 9 cut(s) 202, 356, 560, 795, 2026, 2665, 2693, 3167, 3357
Hpy8I GTNNAC 9 cut(s) 202, 356, 560, 795, 2026, 2665, 2693, 3167, 3357
Hpy99I CGWCG 2 cut(s) 237, 2923
HpyCH4III ACNGT 8 cut(s) 177, 768, 1268, 1481, 2303, 3121, 3174, 3621
HpyCH4IV ACGT 5 cut(s) 198, 470, 1735, 2348, 2667
HpyF10VI GCNNNNNNNGC 8 cut(s) 243, 1357, 1537, 1622, 1631, 1839, 1943, 3386
HpySE526I ACGT 5 cut(s) 198, 470, 1735, 2348, 2667
Hsp92I GRCGYC 1 cut(s) 2439
HspAI GCGC 3 cut(s) 495, 1944, 2541
KflI GGGWCCC 1 cut(s) 2963
KpnI GGTACC 1 cut(s) 1406
Ksp22I TGATCA 1 cut(s) 3523
LguI GCTCTTC 2 cut(s) 1053, 1329
LmnI GCTCC 9 cut(s) 436, 907, 1255, 1519, 1564, 1941, 2548, 3084, 3190
Lsp1109I GCAGC 6 cut(s) 1625, 1845, 1877, 2133, 2603, 3328
MabI ACCWGGT 1 cut(s) 2626
MaeI CTAG 5 cut(s) 159, 800, 867, 2037, 3602
MaeII ACGT 5 cut(s) 198, 470, 1735, 2348, 2667
MfeI CAATTG 1 cut(s) 307
MflI RGATCY 6 cut(s) 320, 734, 1112, 2167, 2702, 3112
MhlI GDGCHC 1 cut(s) 1252
MlyI GAGTC 9 cut(s) 83, 290, 624, 2034, 2206, 3033, 3267, 3337, 3419
MmeI TCCRAC 2 cut(s) 3140, 3561
Mph1103I ATGCAT 3 cut(s) 248, 1242, 1903
MroXI GAANNNNTTC 2 cut(s) 861, 3690
MslI CAYNNNNRTG 5 cut(s) 701, 1175, 1245, 2815, 3102
MspA1I CMGCKG 3 cut(s) 1868, 2146, 3227
MspCI CTTAAG 1 cut(s) 1678
MspI CCGG 4 cut(s) 66, 3088, 3156, 3323
MunI CAATTG 1 cut(s) 307
Mva1269I GAATGC 3 cut(s) 1432, 1835, 2322
MvaI CCWGG 8 cut(s) 783, 1275, 1323, 1562, 2175, 2580, 2628, 3665
MvnI CGCG 2 cut(s) 348, 495
MwoI GCNNNNNNNGC 8 cut(s) 243, 1357, 1537, 1622, 1631, 1839, 1943, 3386
NciI CCSGG 3 cut(s) 66, 3089, 3324
NcoI CCATGG 2 cut(s) 253, 1534
NdeI CATATG 4 cut(s) 511, 672, 1838, 2353
NmeAIII GCCGAG 1 cut(s) 1522
NmuCI GTSAC 7 cut(s) 151, 1363, 2359, 2423, 2804, 3635, 3667
NsiI ATGCAT 3 cut(s) 248, 1242, 1903
NspI RCATGY 3 cut(s) 863, 1205, 1905
OliI CACNNNNGTG 1 cut(s) 3102
PaeI GCATGC 1 cut(s) 1905
PaeR7I CTCGAG 2 cut(s) 603, 1455
PagI TCATGA 2 cut(s) 15, 1591
PceI AGGCCT 2 cut(s) 3582, 3663
PciI ACATGT 1 cut(s) 859
PciSI GCTCTTC 2 cut(s) 1053, 1329
PctI GAATGC 3 cut(s) 1432, 1835, 2322
PdmI GAANNNNTTC 2 cut(s) 861, 3690
PfeI GAWTC 7 cut(s) 287, 638, 2288, 2645, 2991, 3457, 3691
PflMI CCANNNNNTGG 1 cut(s) 2765
PfoI TCCNGGA 1 cut(s) 2173
PkrI GCNGC 7 cut(s) 1615, 1835, 1867, 2148, 2593, 3318, 3532
PleI GAGTC 9 cut(s) 82, 289, 623, 2034, 2205, 3032, 3266, 3336, 3419
PmaCI CACGTG 2 cut(s) 1736, 2668
PmlI CACGTG 2 cut(s) 1736, 2668
PpsI GAGTC 9 cut(s) 82, 289, 623, 2034, 2205, 3032, 3266, 3336, 3419
Ppu21I YACGTR 2 cut(s) 1736, 2668
PpuMI RGGWCCY 3 cut(s) 418, 1710, 2963
PscI ACATGT 1 cut(s) 859
PshAI GACNNNNGTC 1 cut(s) 3429
Psp5II RGGWCCY 3 cut(s) 418, 1710, 2963
Psp6I CCWGG 8 cut(s) 781, 1273, 1321, 1560, 2173, 2578, 2626, 3663
PspCI CACGTG 2 cut(s) 1736, 2668
PspFI CCCAGC 2 cut(s) 903, 3237
PspGI CCWGG 8 cut(s) 781, 1273, 1321, 1560, 2173, 2578, 2626, 3663
PspPI GGNCC 7 cut(s) 418, 785, 1710, 2756, 2941, 2963, 3167
PspPPI RGGWCCY 3 cut(s) 418, 1710, 2963
PspXI VCTCGAGB 2 cut(s) 603, 1455
PsrI GAACNNNNNNTAC 4 cut(s) 1635, 1667, 3605, 3637
PstNI CAGNNNCTG 4 cut(s) 155, 1868, 2585, 3237
PsuI RGATCY 6 cut(s) 320, 734, 1112, 2167, 2702, 3112
PvuII CAGCTG 3 cut(s) 1868, 2146, 3227
RseI CAYNNNNRTG 5 cut(s) 701, 1175, 1245, 2815, 3102
SapI GCTCTTC 2 cut(s) 1053, 1329
SatI GCNGC 7 cut(s) 1614, 1834, 1866, 2147, 2592, 3317, 3531
Sau96I GGNCC 7 cut(s) 418, 785, 1710, 2756, 2941, 2963, 3167
ScaI AGTACT 2 cut(s) 3147, 3419
SchI GAGTC 9 cut(s) 83, 290, 624, 2034, 2206, 3033, 3267, 3337, 3419
SduI GDGCHC 1 cut(s) 1252
SexAI ACCWGGT 1 cut(s) 2626
SfcI CTRYAG 2 cut(s) 915, 1548
Sfr274I CTCGAG 2 cut(s) 603, 1455
SinI GGWCC 6 cut(s) 418, 785, 1710, 2941, 2963, 3167
SlaI CTCGAG 2 cut(s) 603, 1455
SmiMI CAYNNNNRTG 5 cut(s) 701, 1175, 1245, 2815, 3102
SmlI CTYRAG 9 cut(s) 563, 603, 1455, 1678, 1727, 1994, 2271, 2337, 2834
SmoI CTYRAG 9 cut(s) 563, 603, 1455, 1678, 1727, 1994, 2271, 2337, 2834
SphI GCATGC 1 cut(s) 1905
SseBI AGGCCT 2 cut(s) 3582, 3663
SsiI CCGC 7 cut(s) 348, 415, 943, 1625, 2953, 2986, 3530
SspI AATATT 1 cut(s) 938
SspMI CTAG 5 cut(s) 159, 800, 867, 2037, 3602
StuI AGGCCT 2 cut(s) 3582, 3663
StyI CCWWGG 4 cut(s) 253, 333, 1160, 1534
TaaI ACNGT 8 cut(s) 177, 768, 1268, 1481, 2303, 3121, 3174, 3621
TaiI ACGT 5 cut(s) 201, 473, 1738, 2351, 2670
TaqII GACCGA 2 cut(s) 309, 2958
TatI WGTACW 8 cut(s) 1065, 1221, 2662, 3145, 3373, 3417, 3451, 3621
TauI GCSGC 1 cut(s) 3533
TfiI GAWTC 7 cut(s) 287, 638, 2288, 2645, 2991, 3457, 3691
TscAI CASTG 9 cut(s) 156, 405, 1021, 1141, 2601, 2770, 2830, 3177, 3624
TseFI GTSAC 7 cut(s) 151, 1363, 2359, 2423, 2804, 3635, 3667
TseI GCWGC 6 cut(s) 1613, 1833, 1865, 2146, 2591, 3316
Tsp45I GTSAC 7 cut(s) 151, 1363, 2359, 2423, 2804, 3635, 3667
TspDTI ATGAA 9 cut(s) 123, 1143, 1343, 1992, 2445, 2637, 2868, 3202, 3314
TspGWI ACGGA 3 cut(s) 715, 2610, 3478
TspRI CASTG 9 cut(s) 156, 405, 1021, 1141, 2601, 2770, 2830, 3177, 3624
Van91I CCANNNNNTGG 1 cut(s) 2765
Vha464I CTTAAG 1 cut(s) 1678
VpaK11BI GGWCC 6 cut(s) 418, 785, 1710, 2941, 2963, 3167
XagI CCTNNNNNAGG 1 cut(s) 870
XapI RAATTY 5 cut(s) 593, 1072, 1292, 1763, 1922
XbaI TCTAGA 1 cut(s) 799
XceI RCATGY 3 cut(s) 863, 1205, 1905
XcmI CCANNNNNNNNNTGG 1 cut(s) 3388
XhoI CTCGAG 2 cut(s) 603, 1455
XmiI GTMKAC 1 cut(s) 2025
XmnI GAANNNNTTC 2 cut(s) 861, 3690
XspI CTAG 5 cut(s) 159, 800, 867, 2037, 3602
ZrmI AGTACT 2 cut(s) 3147, 3419
Zsp2I ATGCAT 3 cut(s) 248, 1242, 1903
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.