RLG00000025815

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0002633
Physical Location & Seq
Forward (+)
48459507 .. 48468830
9324 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025815

Sequence Viewer

Length: 3669 bp
ATGGGGACAGAACTCATGAGAGTTTGCATAAAGGAAGACAATGATGAATTTCCATCAGTGCCACCGGGATTTGAGTCATTCACATCTTTCAGCTTAAAAAGGGTGAATGAAAGTGAGAAACAAGATAGTGAAAACCTAATCAGTTGCTGTGCCACTACCAGTACTTCTGAATCACTGTCTGTTCAGATGGAAACTGGTGATAATGTTGCTGAAGTTGCAAAGAGGTCTCAACGGCGGAGGCCATGGATAAACAATAGACGGTATTATAACAATGCTGAGGATGAGTTTGATTATGAGAGCCTTGATCAAAATTTTCTTCTAAGGTCCCTTCTTCCTAAAGGGGTTATACGCGGATGTCCAGAGTGTAGTACCTGCCAAAAGGTATCTGCAAGATGGCATCCAGAGGATGGTCAAAGGCCTGACATCCAGGATGCTCCAGTATTCAGACCAACTGAAGAGGAGTTCAAAGATACTTTGAAATACATAGCGAGCATACGCCCAAAAGCTGAACCATATGGTATTTGTCGCATTGTTCCTCCCTCTTCATGGAGACCTCCCTGTCCTCTCAAGGAAAAGAATGTTTGGGAGACTTCGAAGTTTGCTACTCGCGTCCAAAGAGTCGACAAACTTCAGAATCGAGATTCAATGAGAAAGATGCCAAAAGGTCATAATCATACGAGGAAGAAAAGGAGAAGATGCACACGAATGGGGGCTGATTGTCCTGCTGGTGGTAGAGGATCTGGGGATGATGGAATTTGTGAGGCAGAAAGGTTTGGGTTTGAACCTGGTCCAGAGTTTACTTTAGGAGCGTTTGAAAAATATGCTGATGACTTCAAGACCCAATACTTTAGCAAGAATGAACACTTTACTGATATAGGAGGTCACTTGAGTGAAGTCAAAGAGCGCTGGGAGCCATCTGTGGAAAATATTGAGGGTGAATATTGGCGGATGGTGGAGAAACCTACGGAAGAAATTGAGGTTCTATATGGGGCTGATTTGGAAACAGGAGTTTTCGGAAGTGGTTTTCCCAAGATATCCGGTCAGGATGGTTTTACTTCAGAGGAGCAGTATATAACATCTGGCTGGAACTTGAACAACTTTCCTAGACTGCCTGGATCTGTCCTTTCTTACGAAAGCAGTGATATATCTGGTGTTCTGGTGCCATGGTTATATATAGGGATGTGCTTTTCTTCCTTTTGCTGGCATGTTGAAGATCACCACTTGTACTCTTTAAATTACATGCATTGGGGTGCTCCGAAGCTGTGGTATGGTATCCCTGGTAGTGATGCCTGTAAATTTGAAGAGGTTATGAGAAAGCACCTCCCTGACCTATTTGAAGAACAACCTGACTTGCTTCATAAGCTGGTAACACAGCTCTCCCCCTCTATACTGAAATCTGATGGGGTACCAGTGTACAGATGTTGTCAGAATCCTGGAGAGTTTGTCCTGACCTTTCCTCGAGCTTACCATTCGGGGTTCAACTGTGGTTTCAATTGTGCAGAGGCAGTTAACGTAGCTCCTGTTGATTGGTTGCCTCATGGACAGGTTGCTATAGAGCTATACCAAGAGCAGGGACGAAAGACATCCATTTCCCATGATAAATTGTTGCTTGGCGCTGCAAGGGAGGCAGTAAGGGCACATTGGGAGCTCAATTTACTGAAGAAGAACACTTCTGATAACTTAAGATGGAAAAATGTCTGTGGAAAGGATGGGATCTTAGCCAAAGTTCTCAAGGCACGTGTTGAGATGGAGCGTGTGAGGAGGGAATTTCTTTGCAATTCTTCCCAGGCATTGAAGATGGAGAGCAATTTTGATGCCACGAGTGAGAGGGAATGCAGTGTATGCTTTTTTGATTTGCATCTGTCAGCAGCTGGCTGTCATCAATGTTCCCCAGATAGATATGCATGCTTGAATCATGCAAAGCAATTCTGTTCGTGTGCTTGGAGCACTAAGTTTTTCCTCTTCCGTTATGACCTAGATGAATTAAACATCCTTCTCGAAGCACTGGAAGGAAAGTTGAGTGCAGTATACAGATGGGCTAGACTAGATCTTGGGTTGGCCTTGAGTTCTTATATTGGCAAAGACATAAAAGTTGGTAAATTATCGTATGCCTCCAAGAGTACTATACTTGAAGAAGTGAGCTCACAGCCACAAAGCAATTGCTTTAAAGATCCACCAGGGAAAGAAATCTCTAAAGATGGTCCTGAAAGGTCAACTGGGAGAGAAGAATCATTTCTTAGCACTGCCAACTCTCTGCAGGTTTGCCAGTTGTCTCAAGAAGACACATCATATGCTTTGAACTCGGCTGAAAGGGAATCTGGGTTGAAGATGACTTCAGTTGACAATATTATACTTCTTAGTGATGATGAGAGTGATGAGCCAAAGAACCTACACTCAGACAGCCCTACAGCGATATCTTCTGCTAACCAGTTAGAACTTCCCAATAGATTGGTGGCTTCTGATGGAAAAGTGAGCCCTTGTAATGTTGAGAAAGCTGCAGTCTTAAATATGCCTGTGACTGACGCAGCTGTGATGGTTGAAAAAGTTATTAGTCCGTTGGCTAGTGAAGGAAAAAAGGATTTTTCATCCCGTTCTTATTCTCATATTATGAATGTGAAAGATGAACAGGAGAATGATGGACAATCACGATCCAATTCACCTAACCTTTCATCCAAATTAGGTTCTGTTGGGGCAGAACATGGACCAGACACTTGCCATATTAGTGAACCCAAGGTTGCTATTTCCAGGAGTGACCCGAAAGATTCACAGCCATGTCACAGTATAAAACCTGAGAACGAGGATAGACATGAAAAGATAGGAAGAAATGCCGATGCAAATATAGTGGATAGTGTAAGAACTGCAATGGGAAACCCATCGTCCAGCCAAAACAATTTGGACAGATATTATCGCCAGAAGGGTCCCCGTATAGCAAAGGTAGTGCGGAGGATCACCTGCATTGTTGAGCCCCTAGAATTTGGAGTCGTGCTCTCTGGAAAGTCATGGTGTAACAGCCAAGCCATTTTTCCAAAAGGATTTAGGAGCCGGGTTAAGTACATTAGTGTTGTCGACCCTACTGTTAGGTGTTATTATGTCTCAGAAGTACTTGATGCAAGACAGACCGGACCTCTTTTCATGGTTTCTTTGGATCAGTGTCCAGGCGAGGTTTTCATCCATAATTCAGTTGGTAGATGCTGGGAAATGGTGAGAGAGAGAGTCAATCAAGAAATCTCGAGGCACCATAAGTTGGGAAGACCAAACCTTCCTCCTTTGCAGCCACCTGGGAGTCTTGATGGCTTTGAAATGTTTGGTTTTACTTCACCAGTTATTGTACAGGCCATAGAGGCAATGGATAGGAACCGCGTTTGTTCCGAGTACTGGGACTCTCGTCCCTACTCCCGACCTCAAGTGCAGATACCACAAAAAGCTCAATCTGAAGAAAGTGGTGAAAACTTGAATAAGATAACGGCGGGAAGGAACGCTCATGAGGCTGCCGGCATTAATCTCTTGACTAGTGGGGTTGATACACTACTCGGAGGCCTGTTCAAGAAGGCTAACCTAGAAGAATTAAACTCACTGTACAGCATCTTAAATGACAATCAGCAGACCGTCGGGCGAGGCCTAGTGACACGACTTCTTAATGAAGAGATTCATACTCGTCGTCCAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000976 GO:0001067 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0003824 GO:0005488 GO:0005506 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006325 GO:0006355 GO:0006464 GO:0006479 GO:0006482 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008198 GO:0008213 GO:0008214 GO:0008276 GO:0009314 GO:0009416 GO:0009628 GO:0009648 GO:0009791 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009910 GO:0009987 GO:0010216 GO:0010228 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0016577 GO:0016740 GO:0016741 GO:0019219 GO:0019222 GO:0019538 GO:0022414 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0032451 GO:0032452 GO:0032453 GO:0032501 GO:0032502 GO:0034641 GO:0034720 GO:0036211 GO:0040008 GO:0040009 GO:0040010 GO:0040029 GO:0042054 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045814 GO:0045892 GO:0045927 GO:0045934 GO:0046483 GO:0046872 GO:0046914 GO:0048518 GO:0048519 GO:0048523 GO:0048573 GO:0048579 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048586 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051172 GO:0051239 GO:0051241 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0140096 GO:0140110 GO:1901360 GO:1901363 GO:1901564 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000026 GO:2000028 GO:2000112 GO:2000113 GO:2000241 GO:2000242 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FYRC FYRN JmjC JmjN zf-C5HC2

Protein Analysis

1223

Amino Acids

137.43

Weight (kDa)

5.98

Isoelectric Point (pI)

52.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JmjN PF02375 146 - 179 4.6e-18 jmjN domain
JmjC PF02373 389 - 505 4.7e-46 JmjC domain, hydroxylase
zf-C5HC2 PF02928 612 - 664 5.2e-16 C5HC2 zinc finger
FYRN PF05964 992 - 1036 2.1e-11 F/Y-rich N-terminus
FYRC PF05965 1041 - 1125 2.1e-20 F/Y rich C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 267
AarI CACCTGC 1 cut(s) 2960
AasI GACNNNNNNGTC 1 cut(s) 558
Acc36I ACCTGC 3 cut(s) 380, 2248, 2960
Acc65I GGTACC 1 cut(s) 1405
AccB1I GGYRCC 3 cut(s) 1159, 1405, 3234
AccB7I CCANNNNNTGG 2 cut(s) 407, 3244
AccI GTMKAC 3 cut(s) 621, 2028, 3066
AccII CGCG 3 cut(s) 351, 609, 3360
AciI CCGC 6 cut(s) 235, 351, 946, 2941, 3358, 3467
AclWI GGATC 7 cut(s) 745, 1123, 1721, 2165, 2643, 2954, 3153
AcsI RAATTY 6 cut(s) 47, 310, 753, 1295, 1766, 2972
AcuI CTGAAG 7 cut(s) 231, 474, 614, 1041, 1679, 2319, 3453
AcvI CACGTG 1 cut(s) 1739
AfeI AGCGCT 1 cut(s) 905
AfiI CCNNNNNNNGG 7 cut(s) 407, 546, 728, 1200, 1570, 3244, 3375
AflII CTTAAG 1 cut(s) 1681
AflIII ACRYGT 1 cut(s) 1738
AhlI ACTAGT 1 cut(s) 3509
AjuI GAANNNNNNNTTGG 2 cut(s) 3006, 3038
AleI CACNNNNGTG 1 cut(s) 888
Alw21I GWGCWC 5 cut(s) 1255, 1650, 1949, 2144, 2988
Alw26I GTCTC 5 cut(s) 231, 544, 581, 2277, 3097
AlwI GGATC 7 cut(s) 745, 1123, 1721, 2165, 2643, 2954, 3153
AlwNI CAGNNNCTG 2 cut(s) 147, 1871
Ama87I CYCGRG 2 cut(s) 1458, 3229
Aor51HI AGCGCT 1 cut(s) 905
AoxI GGCC 6 cut(s) 239, 416, 2058, 3333, 3535, 3616
ApeKI GCWGC 6 cut(s) 1616, 1868, 2495, 2525, 3271, 3488
ApoI RAATTY 6 cut(s) 47, 310, 753, 1295, 1766, 2972
AseI ATTAAT 1 cut(s) 3498
Asp700I GAANNNNTTC 2 cut(s) 2232, 3645
Asp718I GGTACC 1 cut(s) 1405
AspLEI GCGC 2 cut(s) 906, 1616
AspS9I GGNCC 6 cut(s) 324, 788, 2201, 2702, 2918, 3122
AsuC2I CCSGG 2 cut(s) 66, 3044
AsuHPI GGTGA 9 cut(s) 115, 209, 947, 1208, 2649, 2941, 3214, 3309, 3455
AsuII TTCGAA 1 cut(s) 593
AvaI CYCGRG 2 cut(s) 1458, 3229
AvaII GGWCC 6 cut(s) 324, 788, 2201, 2702, 2918, 3122
BaeGI GKGCMC 1 cut(s) 1639
BaeI ACNNNNGTAYC 2 cut(s) 1397, 1430
BanI GGYRCC 3 cut(s) 1159, 1405, 3234
BanII GRGCYC 4 cut(s) 1650, 2144, 2477, 2967
BauI CACGAG 1 cut(s) 1819
BbrPI CACGTG 1 cut(s) 1739
BbsI GAAGAC 3 cut(s) 42, 2286, 3256
Bbv12I GWGCWC 5 cut(s) 1255, 1650, 1949, 2144, 2988
BbvCI CCTCAGC 1 cut(s) 276
BbvI GCAGC 6 cut(s) 1603, 1880, 2482, 2537, 3283, 3475
BceAI ACGGC 2 cut(s) 248, 3480
BciVI GTATCC 1 cut(s) 1283
BclI TGATCA 1 cut(s) 304
BcnI CCSGG 2 cut(s) 66, 3044
BcoDI GTCTC 5 cut(s) 231, 544, 581, 2277, 3097
BcuI ACTAGT 1 cut(s) 3509
BfaI CTAG 9 cut(s) 1104, 1976, 2040, 2045, 2562, 2969, 3510, 3557, 3620
BfmI CTRYAG 4 cut(s) 1551, 2255, 2406, 2496
BfoI RGCGCY 2 cut(s) 907, 1617
BfrI CTTAAG 1 cut(s) 1681
BfuAI ACCTGC 3 cut(s) 380, 2248, 2960
BfuI GTATCC 1 cut(s) 1283
BglI GCCNNNNNGGC 1 cut(s) 3341
BglII AGATCT 1 cut(s) 2047
BisI GCNGC 6 cut(s) 1617, 1869, 2496, 2526, 3272, 3489
BlsI GCNGC 6 cut(s) 1618, 1870, 2497, 2527, 3273, 3490
BmcAI AGTACT 4 cut(s) 163, 2122, 3102, 3374
Bme18I GGWCC 6 cut(s) 324, 788, 2201, 2702, 2918, 3122
BmeT110I CYCGRG 2 cut(s) 1458, 3229
BmgT120I GGNCC 6 cut(s) 324, 788, 2201, 2702, 2918, 3122
BmiI GGNNCC 9 cut(s) 326, 912, 1161, 1407, 2919, 2920, 3041, 3236, 3356
BmrI ACTGGG 2 cut(s) 2226, 3385
BmuI ACTGGG 2 cut(s) 2226, 3385
BoxI GACNNNNGTC 1 cut(s) 3384
BpiI GAAGAC 3 cut(s) 42, 2286, 3256
BplI GAGNNNNNCTC 2 cut(s) 2970, 3002
BpmI CTGGAG 2 cut(s) 420, 1455
Bpu10I CCTNAGC 1 cut(s) 276
Bpu14I TTCGAA 1 cut(s) 593
BpuEI CTTGAG 6 cut(s) 551, 907, 1715, 2083, 2259, 3387
BpuMI CCSGG 2 cut(s) 66, 3044
BsaAI YACGTR 1 cut(s) 1739
BsaBI GATNNNNATC 1 cut(s) 1857
BsaI GGTCTC 2 cut(s) 231, 544
BsaJI CCNNGG 7 cut(s) 242, 1163, 1276, 1785, 2177, 2730, 3278
BsaWI WCCGGW 2 cut(s) 1037, 3119
BsaXI ACNNNNNCTCC 2 cut(s) 1428, 1458
Bsc4I CCNNNNNNNGG 7 cut(s) 407, 546, 728, 1200, 1570, 3244, 3375
Bse118I RCCGGY 1 cut(s) 3491
Bse3DI GCAATG 2 cut(s) 2868, 3351
Bse8I GATNNNNATC 1 cut(s) 1857
BseDI CCNNGG 7 cut(s) 242, 1163, 1276, 1785, 2177, 2730, 3278
BseJI GATNNNNATC 1 cut(s) 1857
BseLI CCNNNNNNNGG 7 cut(s) 407, 546, 728, 1200, 1570, 3244, 3375
BseMI GCAATG 2 cut(s) 2868, 3351
BseMII CTCAG 4 cut(s) 267, 2409, 2781, 3108
BseRI GAGGAG 3 cut(s) 473, 1076, 1774
BseSI GKGCMC 1 cut(s) 1639
BseXI GCAGC 6 cut(s) 1603, 1880, 2482, 2537, 3283, 3475
BseYI CCCAGC 2 cut(s) 906, 3192
BsgI GTGCAG 3 cut(s) 1518, 2043, 3428
Bsh1236I CGCG 3 cut(s) 351, 609, 3360
BshFI GGCC 6 cut(s) 241, 418, 2060, 3335, 3537, 3618
BshNI GGYRCC 3 cut(s) 1159, 1405, 3234
BsiHKAI GWGCWC 5 cut(s) 1255, 1650, 1949, 2144, 2988
BsiHKCI CYCGRG 2 cut(s) 1458, 3229
BsiSI CCGG 5 cut(s) 65, 1038, 3043, 3120, 3492
BslFI GGGAC 6 cut(s) 19, 310, 1587, 2904, 3372, 3392
BslI CCNNNNNNNGG 7 cut(s) 407, 546, 728, 1200, 1570, 3244, 3375
BsmAI GTCTC 5 cut(s) 231, 544, 581, 2277, 3097
BsmFI GGGAC 6 cut(s) 19, 310, 1587, 2904, 3372, 3392
BsmI GAATGC 1 cut(s) 1838
BsnI GGCC 6 cut(s) 241, 418, 2060, 3335, 3537, 3618
Bso31I GGTCTC 2 cut(s) 231, 544
BsoBI CYCGRG 2 cut(s) 1458, 3229
Bsp119I TTCGAA 1 cut(s) 593
Bsp1286I GDGCHC 8 cut(s) 1255, 1639, 1650, 1949, 2144, 2477, 2967, 2988
Bsp1407I TGTACA 3 cut(s) 1413, 3328, 3576
Bsp19I CCATGG 2 cut(s) 242, 1163
BspACI CCGC 6 cut(s) 235, 351, 946, 2941, 3358, 3467
BspANI GGCC 6 cut(s) 241, 418, 2060, 3335, 3537, 3618
BspCNI CTCAG 4 cut(s) 268, 2408, 2782, 3107
BspFNI CGCG 3 cut(s) 351, 609, 3360
BspHI TCATGA 2 cut(s) 15, 3481
BspLI GGNNCC 9 cut(s) 326, 912, 1161, 1407, 2919, 2920, 3041, 3236, 3356
BspMAI CTGCAG 2 cut(s) 2259, 2500
BspMI ACCTGC 3 cut(s) 380, 2248, 2960
BspPI GGATC 7 cut(s) 745, 1123, 1721, 2165, 2643, 2954, 3153
BspT104I TTCGAA 1 cut(s) 593
BspT107I GGYRCC 3 cut(s) 1159, 1405, 3234
BspTI CTTAAG 1 cut(s) 1681
BspTNI GGTCTC 2 cut(s) 231, 544
BsrDI GCAATG 2 cut(s) 2868, 3351
BsrFI RCCGGY 1 cut(s) 3491
BsrGI TGTACA 3 cut(s) 1413, 3328, 3576
BssAI RCCGGY 1 cut(s) 3491
BssECI CCNNGG 7 cut(s) 242, 1163, 1276, 1785, 2177, 2730, 3278
BssNAI GTATAC 1 cut(s) 2029
BssSI CACGAG 1 cut(s) 1819
BssT1I CCWWGG 3 cut(s) 242, 1163, 2730
Bst1107I GTATAC 1 cut(s) 2029
Bst2BI CACGAG 1 cut(s) 1819
Bst4CI ACNGT 7 cut(s) 177, 261, 1484, 2780, 3076, 3576, 3607
Bst6I CTCTTC 5 cut(s) 450, 547, 1296, 1967, 3636
BstAFI CTTAAG 1 cut(s) 1681
BstAPI GCANNNNNTGC 1 cut(s) 1842
BstAUI TGTACA 3 cut(s) 1413, 3328, 3576
BstBAI YACGTR 1 cut(s) 1739
BstBI TTCGAA 1 cut(s) 593
BstC8I GCNNGC 5 cut(s) 490, 1202, 1873, 1906, 3493
BstDEI CTNAG 9 cut(s) 276, 320, 1717, 1950, 2237, 2357, 2395, 2790, 3094
BstDSI CCRYGG 2 cut(s) 242, 1163
BstFNI CGCG 3 cut(s) 351, 609, 3360
BstH2I RGCGCY 2 cut(s) 907, 1617
BstHHI GCGC 2 cut(s) 906, 1616
BstMAI GTCTC 5 cut(s) 231, 544, 581, 2277, 3097
BstMWI GCNNNNNNNGC 8 cut(s) 215, 910, 1360, 1625, 1634, 1842, 3341, 3485
BstNSI RCATGY 3 cut(s) 1208, 1243, 1908
BstPAI GACNNNNGTC 1 cut(s) 3384
BstSFI CTRYAG 4 cut(s) 1551, 2255, 2406, 2496
BstSLI GKGCMC 1 cut(s) 1639
BstUI CGCG 3 cut(s) 351, 609, 3360
BstV1I GCAGC 6 cut(s) 1603, 1880, 2482, 2537, 3283, 3475
BstV2I GAAGAC 3 cut(s) 42, 2286, 3256
BstX2I RGATCY 5 cut(s) 737, 1115, 1713, 2047, 2170
BstXI CCANNNNNNTGG 1 cut(s) 2449
BstYI RGATCY 5 cut(s) 737, 1115, 1713, 2047, 2170
BstZ17I GTATAC 1 cut(s) 2029
BsuI GTATCC 1 cut(s) 1283
BsuRI GGCC 6 cut(s) 241, 418, 2060, 3335, 3537, 3618
BtgI CCRYGG 2 cut(s) 242, 1163
BtsI GCAGTG 3 cut(s) 1144, 1843, 2241
BtsIMutI CAGTG 9 cut(s) 63, 173, 1144, 1416, 1843, 2003, 2241, 3155, 3572
BveI ACCTGC 3 cut(s) 380, 2248, 2960
Cac8I GCNNGC 5 cut(s) 490, 1202, 1873, 1906, 3493
CaiI CAGNNNCTG 2 cut(s) 147, 1871
CciI TCATGA 2 cut(s) 15, 3481
CfoI GCGC 2 cut(s) 906, 1616
Cfr10I RCCGGY 1 cut(s) 3491
Cfr13I GGNCC 6 cut(s) 324, 788, 2201, 2702, 2918, 3122
CseI GACGC 2 cut(s) 598, 2531
CsiI ACCWGGT 1 cut(s) 784
CspCI CAANNNNNGTGG 2 cut(s) 2823, 2858
DdeI CTNAG 9 cut(s) 276, 320, 1717, 1950, 2237, 2357, 2395, 2790, 3094
DraI TTTAAA 2 cut(s) 1233, 2167
DrdI GACNNNNNNGTC 1 cut(s) 558
DseDI GACNNNNNNGTC 1 cut(s) 558
Eam1104I CTCTTC 5 cut(s) 450, 547, 1296, 1967, 3636
EarI CTCTTC 5 cut(s) 450, 547, 1296, 1967, 3636
EciI GGCGGA 2 cut(s) 250, 961
Ecl136II GAGCTC 2 cut(s) 1648, 2142
Eco130I CCWWGG 3 cut(s) 242, 1163, 2730
Eco147I AGGCCT 3 cut(s) 418, 3537, 3618
Eco24I GRGCYC 4 cut(s) 1650, 2144, 2477, 2967
Eco31I GGTCTC 2 cut(s) 231, 544
Eco32I GATATC 2 cut(s) 1035, 2415
Eco47I GGWCC 6 cut(s) 324, 788, 2201, 2702, 2918, 3122
Eco47III AGCGCT 1 cut(s) 905
Eco53kI GAGCTC 2 cut(s) 1648, 2142
Eco57I CTGAAG 7 cut(s) 231, 474, 614, 1041, 1679, 2319, 3453
Eco72I CACGTG 1 cut(s) 1739
Eco88I CYCGRG 2 cut(s) 1458, 3229
EcoICRI GAGCTC 2 cut(s) 1648, 2142
EcoO109I RGGNCCY 2 cut(s) 324, 2918
EcoRV GATATC 2 cut(s) 1035, 2415
EcoT14I CCWWGG 3 cut(s) 242, 1163, 2730
EcoT22I ATGCAT 2 cut(s) 1245, 1906
EcoT38I GRGCYC 4 cut(s) 1650, 2144, 2477, 2967
ErhI CCWWGG 3 cut(s) 242, 1163, 2730
FaqI GGGAC 6 cut(s) 19, 310, 1587, 2904, 3372, 3392
FauI CCCGC 1 cut(s) 3460
FauNDI CATATG 2 cut(s) 514, 2290
FbaI TGATCA 1 cut(s) 304
FblI GTMKAC 3 cut(s) 621, 2028, 3066
Fnu4HI GCNGC 6 cut(s) 1617, 1869, 2496, 2526, 3272, 3489
FriOI GRGCYC 4 cut(s) 1650, 2144, 2477, 2967
Fsp4HI GCNGC 6 cut(s) 1617, 1869, 2496, 2526, 3272, 3489
FspBI CTAG 9 cut(s) 1104, 1976, 2040, 2045, 2562, 2969, 3510, 3557, 3620
GlaI GCGC 2 cut(s) 905, 1615
GluI GCNGC 6 cut(s) 1617, 1869, 2496, 2526, 3272, 3489
GsaI CCCAGC 2 cut(s) 910, 3196
GsuI CTGGAG 2 cut(s) 420, 1455
HaeII RGCGCY 2 cut(s) 907, 1617
HaeIII GGCC 6 cut(s) 241, 418, 2060, 3335, 3537, 3618
HapII CCGG 5 cut(s) 65, 1038, 3043, 3120, 3492
HgaI GACGC 2 cut(s) 598, 2531
HhaI GCGC 2 cut(s) 906, 1616
Hin6I GCGC 2 cut(s) 904, 1614
HinP1I GCGC 2 cut(s) 904, 1614
HincII GTYRAC 5 cut(s) 622, 1510, 2214, 2341, 3067
HindII GTYRAC 5 cut(s) 622, 1510, 2214, 2341, 3067
HpaI GTTAAC 1 cut(s) 1510
HpaII CCGG 5 cut(s) 65, 1038, 3043, 3120, 3492
HphI GGTGA 9 cut(s) 115, 209, 947, 1208, 2649, 2941, 3214, 3309, 3455
Hpy166II GTNNAC 9 cut(s) 622, 798, 1414, 1510, 2029, 2214, 2341, 2726, 3067
Hpy8I GTNNAC 9 cut(s) 622, 798, 1414, 1510, 2029, 2214, 2341, 2726, 3067
Hpy99I CGWCG 2 cut(s) 3611, 3660
HpyAV CCTTC 8 cut(s) 338, 2003, 2003, 2561, 2908, 3269, 3465, 3541
HpyCH4III ACNGT 7 cut(s) 177, 261, 1484, 2780, 3076, 3576, 3607
HpyCH4IV ACGT 2 cut(s) 1512, 1738
HpyF10VI GCNNNNNNNGC 8 cut(s) 215, 910, 1360, 1625, 1634, 1842, 3341, 3485
HpyF3I CTNAG 9 cut(s) 276, 320, 1717, 1950, 2237, 2357, 2395, 2790, 3094
HpySE526I ACGT 2 cut(s) 1512, 1738
HspAI GCGC 2 cut(s) 904, 1614
KflI GGGWCCC 1 cut(s) 2918
KpnI GGTACC 1 cut(s) 1409
KroI GCCGGC 1 cut(s) 3491
KroNI GCCGGC 1 cut(s) 3493
Ksp22I TGATCA 1 cut(s) 304
KspAI GTTAAC 1 cut(s) 1510
Lsp1109I GCAGC 6 cut(s) 1603, 1880, 2482, 2537, 3283, 3475
MabI ACCWGGT 1 cut(s) 784
MaeI CTAG 9 cut(s) 1104, 1976, 2040, 2045, 2562, 2969, 3510, 3557, 3620
MaeII ACGT 2 cut(s) 1512, 1738
MaeIII GTNAC 7 cut(s) 881, 1366, 2515, 2750, 2774, 3005, 3622
MfeI CAATTG 2 cut(s) 1492, 2158
MflI RGATCY 5 cut(s) 737, 1115, 1713, 2047, 2170
MhlI GDGCHC 8 cut(s) 1255, 1639, 1650, 1949, 2144, 2477, 2967, 2988
MlyI GAGTC 6 cut(s) 83, 627, 2988, 3222, 3292, 3374
Mph1103I ATGCAT 2 cut(s) 1245, 1906
MroNI GCCGGC 1 cut(s) 3491
MroXI GAANNNNTTC 2 cut(s) 2232, 3645
MslI CAYNNNNRTG 5 cut(s) 704, 888, 1248, 2721, 2770
MspA1I CMGCKG 2 cut(s) 1871, 2528
MspCI CTTAAG 1 cut(s) 1681
MspI CCGG 5 cut(s) 65, 1038, 3043, 3120, 3492
MunI CAATTG 2 cut(s) 1492, 2158
Mva1269I GAATGC 1 cut(s) 1838
MvnI CGCG 3 cut(s) 351, 609, 3360
MwoI GCNNNNNNNGC 8 cut(s) 215, 910, 1360, 1625, 1634, 1842, 3341, 3485
NaeI GCCGGC 1 cut(s) 3493
NciI CCSGG 2 cut(s) 66, 3044
NcoI CCATGG 2 cut(s) 242, 1163
NdeI CATATG 2 cut(s) 514, 2290
NgoMIV GCCGGC 1 cut(s) 3491
NlaIV GGNNCC 9 cut(s) 326, 912, 1161, 1407, 2919, 2920, 3041, 3236, 3356
NmeAIII GCCGAG 1 cut(s) 2282
NmuCI GTSAC 5 cut(s) 881, 2515, 2750, 2774, 3622
NsiI ATGCAT 2 cut(s) 1245, 1906
NspI RCATGY 3 cut(s) 1208, 1243, 1908
NspV TTCGAA 1 cut(s) 593
OliI CACNNNNGTG 1 cut(s) 888
PaeI GCATGC 1 cut(s) 1908
PaeR7I CTCGAG 2 cut(s) 1458, 3229
PagI TCATGA 2 cut(s) 15, 3481
PaqCI CACCTGC 1 cut(s) 2960
PceI AGGCCT 3 cut(s) 418, 3537, 3618
PctI GAATGC 1 cut(s) 1838
PdiI GCCGGC 1 cut(s) 3493
PdmI GAANNNNTTC 2 cut(s) 2232, 3645
PfeI GAWTC 9 cut(s) 170, 634, 641, 1429, 1912, 2228, 2315, 2762, 3646
PflMI CCANNNNNTGG 2 cut(s) 407, 3244
PfoI TCCNGGA 3 cut(s) 426, 1432, 2744
PkrI GCNGC 6 cut(s) 1618, 1870, 2497, 2527, 3273, 3490
PleI GAGTC 6 cut(s) 82, 626, 2987, 3221, 3291, 3374
PmaCI CACGTG 1 cut(s) 1739
PmlI CACGTG 1 cut(s) 1739
PpsI GAGTC 6 cut(s) 82, 626, 2987, 3221, 3291, 3374
Ppu21I YACGTR 1 cut(s) 1739
PpuMI RGGWCCY 2 cut(s) 324, 2918
PshAI GACNNNNGTC 1 cut(s) 3384
PshBI ATTAAT 1 cut(s) 3498
PsiI TTATAA 1 cut(s) 267
Psp124BI GAGCTC 2 cut(s) 1650, 2144
Psp5II RGGWCCY 2 cut(s) 324, 2918
PspCI CACGTG 1 cut(s) 1739
PspFI CCCAGC 2 cut(s) 906, 3192
PspN4I GGNNCC 9 cut(s) 326, 912, 1161, 1407, 2919, 2920, 3041, 3236, 3356
PspPI GGNCC 6 cut(s) 324, 788, 2201, 2702, 2918, 3122
PspPPI RGGWCCY 2 cut(s) 324, 2918
PspXI VCTCGAGB 1 cut(s) 1458
PstI CTGCAG 2 cut(s) 2259, 2500
PstNI CAGNNNCTG 2 cut(s) 147, 1871
PsuI RGATCY 5 cut(s) 737, 1115, 1713, 2047, 2170
PvuII CAGCTG 2 cut(s) 1871, 2528
RseI CAYNNNNRTG 5 cut(s) 704, 888, 1248, 2721, 2770
SacI GAGCTC 2 cut(s) 1650, 2144
SalI GTCGAC 2 cut(s) 620, 3065
SatI GCNGC 6 cut(s) 1617, 1869, 2496, 2526, 3272, 3489
Sau96I GGNCC 6 cut(s) 324, 788, 2201, 2702, 2918, 3122
ScaI AGTACT 4 cut(s) 163, 2122, 3102, 3374
SchI GAGTC 6 cut(s) 83, 627, 2988, 3222, 3292, 3374
SduI GDGCHC 8 cut(s) 1255, 1639, 1650, 1949, 2144, 2477, 2967, 2988
SexAI ACCWGGT 1 cut(s) 784
SfcI CTRYAG 4 cut(s) 1551, 2255, 2406, 2496
Sfr274I CTCGAG 2 cut(s) 1458, 3229
SfuI TTCGAA 1 cut(s) 593
SinI GGWCC 6 cut(s) 324, 788, 2201, 2702, 2918, 3122
SlaI CTCGAG 2 cut(s) 1458, 3229
SmiMI CAYNNNNRTG 5 cut(s) 704, 888, 1248, 2721, 2770
SmlI CTYRAG 9 cut(s) 566, 886, 1458, 1681, 1730, 2062, 2274, 3229, 3402
SmoI CTYRAG 9 cut(s) 566, 886, 1458, 1681, 1730, 2062, 2274, 3229, 3402
SpeI ACTAGT 1 cut(s) 3509
SphI GCATGC 1 cut(s) 1908
SseBI AGGCCT 3 cut(s) 418, 3537, 3618
SsiI CCGC 6 cut(s) 235, 351, 946, 2941, 3358, 3467
SspI AATATT 3 cut(s) 928, 941, 2347
SspMI CTAG 9 cut(s) 1104, 1976, 2040, 2045, 2562, 2969, 3510, 3557, 3620
SstI GAGCTC 2 cut(s) 1650, 2144
StuI AGGCCT 3 cut(s) 418, 3537, 3618
StyI CCWWGG 3 cut(s) 242, 1163, 2730
TaaI ACNGT 7 cut(s) 177, 261, 1484, 2780, 3076, 3576, 3607
TaiI ACGT 2 cut(s) 1515, 1741
TaqI TCGA 7 cut(s) 593, 621, 637, 1459, 1998, 3066, 3230
TatI WGTACW 9 cut(s) 161, 1224, 1413, 2120, 3051, 3100, 3328, 3372, 3576
TfiI GAWTC 9 cut(s) 170, 634, 641, 1429, 1912, 2228, 2315, 2762, 3646
TscAI CASTG 9 cut(s) 63, 180, 1144, 1416, 1843, 2010, 2248, 3155, 3579
TseFI GTSAC 5 cut(s) 881, 2515, 2750, 2774, 3622
TseI GCWGC 6 cut(s) 1616, 1868, 2495, 2525, 3271, 3488
Tsp45I GTSAC 5 cut(s) 881, 2515, 2750, 2774, 3622
TspGWI ACGGA 3 cut(s) 980, 1955, 2544
TspRI CASTG 9 cut(s) 63, 180, 1144, 1416, 1843, 2010, 2248, 3155, 3579
Van91I CCANNNNNTGG 2 cut(s) 407, 3244
Vha464I CTTAAG 1 cut(s) 1681
VpaK11BI GGWCC 6 cut(s) 324, 788, 2201, 2702, 2918, 3122
VspI ATTAAT 1 cut(s) 3498
XapI RAATTY 6 cut(s) 47, 310, 753, 1295, 1766, 2972
XceI RCATGY 3 cut(s) 1208, 1243, 1908
XcmI CCANNNNNNNNNTGG 3 cut(s) 2449, 3179, 3343
XhoI CTCGAG 2 cut(s) 1458, 3229
XmiI GTMKAC 3 cut(s) 621, 2028, 3066
XmnI GAANNNNTTC 2 cut(s) 2232, 3645
XspI CTAG 9 cut(s) 1104, 1976, 2040, 2045, 2562, 2969, 3510, 3557, 3620
ZrmI AGTACT 4 cut(s) 163, 2122, 3102, 3374
Zsp2I ATGCAT 2 cut(s) 1245, 1906
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.