Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0011329

Physical Location & Seq

Genomic Coordinates Forward (+)

31156250 .. 31164327

Gene Structure 8078 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1232400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3846 bp

ATGGACTCCGTTCTCGCCCTCCGCTCCCTCCCCAGCTGCTTCGCGCCGTCCCGAAATCCCCGCTTCTCAAACTTCGCCGCAAACCGCCGCATCAAAATCCAAACTTTCGCTTCGAAATTCCCGAAAACACCGCGAATTCCACTCTCGATTCCGCGCAATACCCGCCTTTTTAGCCTTCTAGAAGCTTCCAGAAGTGTCGGCTCCGGGGAAGAAGAAAGGCCGGCTCTTTCCGCCGAACGCATTGCCAGGCAGGTAGTGTTGGGGCTGTTCTGCTTTGTTTTCGGGTTTGCGCAGTTTCGGGCGGGTCGGAGTGTTGCGGTCGCGGCGCCGCTGGTTTCGGAGGCGGTTTTGGATAAGGAGGAGGTGAAATATGAGTACTCGGAGTATACGAAGAGGCTGCTGGAGACGGTGGGTGCGTTGCTGAAGAGTGTAGATGAGGTTAGAGGAGGAAATGGGGATGTGAAGCTCGTCGAGGCGGCATGGAAAGCGGTGAGGGGGAAGAAGGATGAGGTGCAGGACGAGATATTGGGTAGGCTCCATGGGGAGCTGCGAGAGTTGAGGAGGGAGAAGGAGGGGCTGGTAAAGCGGTCCGACGAGGTTGTGGCCGAGGTGGTGAAGGTGAAGAGGGAATTGGAGAAGTTGGCGGGCAATGCGGGTGAGGAGAAGTCTAAGGAGATGGAGGAGAGGATGGAAGAGAGGCTGGGGAGTCTGGAGGAGGAGTATAATGGGATTTGGGAGAAGGTTGGAAAGATTGAGGATCGGATTTTGAGGAGAGAGACGGCGGCATTGAGTTATGGAGTGAGGGAACTTTGTTTTATTGAGAGGGAATGTGAGCAGTTGGTTCAGAGTTTTACCCGCCAAATGAGGAGGAAGAATGTTGAGAGTGTGCCAAAAGACTCGGTTACCAAGCTTTCTAAATCTGATATACAGAAAGACTTGGAAAATGCACAAAGAAATAATTTGGAGCAAATGATTCTGCCAAATGTTGTGGAAGTTGATGATCCGGGACCCCTCTTTAATTCGACGGATTTTGCTAAACGTATAAAACAAGGCCTTAAAGAATCAAGGGAGCTGCAAAAAAAGACTGAGGCTCAAATAAGGAAAAATATGAAGAAGTTCGGCAGCGAAAAGCGTTTTCTTGTAAAAACACCAGAGGATGAGGTGGTGAAGGGATTTCCGGAAGTTGAATTGAAGTGGATGTTTGGAGATAAGGAGGTTGTGGTTCCGAAAGCTGCTGGCCTCCATTTGTTCCATGGGTGGAAGAAGTGGCGTGAAGATGCTAAGGCAGACCTTAAAAGAAATCTATTAGAAGATGTTGATTTTGGTAAACAGTATGTGGCCCAGAGACAGGAACTAATTCTTCTGGACCGCGACAGGGTGGTGTCGAAGACGTGGTACAATGAGGAAAAGAATAGATGGGAAATGGATCCAGTAGCTGTTCCTTTCTCCGTGTCAAAGAAACTAGTGGAGCATGCCCGAATTAGGCATGATTGGGGTGCCATGTATATTGCACTCAAGGGCGACGACAAGGAATATTATGTTGACATTAAGGAATTTGAAATGCTATTTGAAGATTTTGGAGGGTTTGATGGGCTGTACATGAAAATGCTTGCCTGTGGTATTCCAACTGCTGTTCACCTGATGTGGATTCCTCTTTCTGAGTTGGATATCCGTCAACAATTTCTCTTGCCCATAAGGCTGTCTCATCAATTGTTTAATGCATTGTGGAATACTAGAGCAGTATCGTATACAAGAGATTGGGTTCTTCAGAAGTTCAAAAATATAAATGACGACATAATGATGACAATAGTTTTTCCTCTTGTGGAGATTATACTACCCTACTCTGTAAGAATACAGTTGGGGATGGCATGGCCCGAGGAAATTGATCAAGCTGTTGACTCAACATGGTACTTGAAATGGCAATCTGAGGCAGAAATGAATCATAAATCCAGAAGAACAGATGACATCCAATGGTATTTTTGGTTTCTCGTGAGAAGCGCCATATACGGATATGTTTTATTTCATCTCTTTCGTTTTATGAAGAGAAAAATTCCCAGATTTCTTGGTTACGGGCCATTACGCAGAGATCCAAACATGCGGAAATTGCAGAGAGTGAAATATTATCTTAATTACCGGGTGAGGACAATTAAAGGTAACAAAAAGGCTGGTGTTGATCCTATAACAAGGGCTTTCGATCAAATGAAGAGGGTGAAGAATCCACCAATACCGTTGAAGGACTTCGCCAGTATTGAATCCATGAAAGAGGAAATTAACGAAGTTGTGGCATTTCTGAAAAATCCTGGTGCATTTCAAGAAATGGGTGCCCGTGCACCTCGGGGGGTTCTGATTGTAGGGGAGAGGGGAACAGGAAAGACATCTCTAGCACTGGCTATAGCTGCTCAAGCTAAGGTGCCTGTTGTTAACATTAAAGCTCAAGAGTTGGAGGCTGGACTGTGGGTTGGGCAAAGTGCATCAAATGTTAGGGAGCTGTTTCAAACAGCGAGAGAACTGGCACCTGTAATCATATTTGTGGAGGATTTTGACCTCTTTGCTGGTGTACGTGGAAAATTTATCCACACTAAAAATCAGGATCACGAGGCTTTTATTAATCAACTGCTTGTGGAACTTGATGGGTTTGAGAAACAAGATGGGGTTGTATTGATGGCTACTACTGGTAATCTCAAGCAAATTGATGAGGCCTTACAGCGCCCTGGTCGCATGGATAGAGTATTTCATCTTCAAAGGCCAACTCAAGCAGAAAGGGAGAAGATATTACATATGGCTGCAAAAGAAACAATGGATAGTGAACTAATTGATTTTGTAGACTGGAGAAAGGTTGCTGAAAAGACAGGTCTTTTGCGACCTATAGAATTAAAGCTGGTGCCTGCATCCTTGGAAGGTAGCGCTTTTAGGAGCAAATTTCTTGATACAGATGAACTTCTGAGCTACTGTAGTTGGTTTGCGACTTTCAGCACTTTCATTCCTGAATGGGCGAGGAAAACCAAAATTGGCAAAAAGCTAAGCAAAATGCTGGTGAATCATCTAGGGCTAGCATTGACTAAAGAAGATCTGCAGAGTGTGGTTGATCTGATGGAGCCATATGGTCAGATAACCAATGGAATCGAACTTCTAAACCCTCCTCTTGAGTGGACAAGGGATACCAAGTTCCCACATGCTGTTTGGGCTGCTGGTCGTGGTCTCATTGCCCTTCTATTGCCAAACTTCGATGTAGTCGATAATATATGGCTGGAGCCATTGTCTTGGCAGGGAATCGGATGCACAAAAATAACCAAGGTGAAAAATGAAGGGTCCGGGAGCGCGAATTCTGAATCAAGATCATATCTTGAAAAGAAGCTTGTATTTTGTTTTGGTTCACATGTTGCATCACAAATGCTACTGCCTTTTGGGGAAGAGAACTTTCTTTCTTCTTCAGAGTTAACGCAGTCACAGGAGATAGCTACACGGATGGTCATTCAGTATGGTTGGGGGCCTGATGATAGTCCGGCAATATATTATCGCACGAATGCGTCTACTGCTTTAAGCATGGGGAACAACCATGAGTATGAGATGGCAGCTAAAGTTGAAAAGATATACGATTTAGCATATTATAAAGCGCAAGAAATGCTTCACAAAAATCGTCGAGTTCTTGAAAAAATAGTTGATGAGTTGCTTGAGTTTGAAATCTTGACTGGAAAGGATTTGCAAAGAATTTTTGAAGAAAATGGAGGTGTTCGGGAAAAAGAGCCCTTTTTCCTTTCTGGATCTCATGATAGGGAGCCACTGTCTGGCAGCTTTCTCGAAGGTGGGAGTGCATCAGGAACAGCTCTTTTAAGCGCAGCAGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004176 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005911 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0009506 GO:0009507 GO:0009526 GO:0009536 GO:0009941 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019538 GO:0030054 GO:0031967 GO:0031975 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0055044 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA Peptidase_M41

Protein Analysis

1282

Amino Acids

146.82

Weight (kDa)

8.33

Isoelectric Point (pI)

38.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AAA	PF00004	781 - 914	2.6e-30	ATPase family associated with various cellular activities (AAA)
Peptidase_M41	PF01434	1062 - 1237	1.2e-11	Peptidase family M41

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0011329)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G04340
fragaria_vesca	FvH4_6g01550 FvH4_6g01550 FvH4_6g01550 FvH4_6g01550 FvH4_6g01550
malus_domestica	MD04G1232400.v1.1
prunus_persica	Prupe.6G352000_v2.0.a1
pyrus_communis	pycom04g20580
rosa_chinensis	RchiOBHm_Chr3g0449311
rosa_laevigata	RLG00000025840
rosa_multiflora	Rmu_sc0007719.1_g000003
rosa_roxburghii	Rroxscaffold_6G00427310
rosa_rugosa	Rorug02G0619600
rosa_samantha	Rh3AG017600 Rh3BG017700 Rh3CG016700 Rh3DG018000
rosa_wichuraiana	Rw1G017600 Rw3G001350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3609
Acc16I	TGCGCA	1 cut(s)	291
Acc36I	ACCTGC	1 cut(s)	241
AccB1I	GGYRCC	6 cut(s)	325, 1496, 2321, 2410, 2512, 2881
AccB7I	CCANNNNNTGG	1 cut(s)	3785
AccBSI	CCGCTC	1 cut(s)	24
AccI	GTMKAC	4 cut(s)	386, 1748, 2823, 3530
AccII	CGCG	6 cut(s)	44, 133, 154, 323, 1371, 3320
AccIII	TCCGGA	1 cut(s)	1177
AclWI	GGATC	8 cut(s)	765, 995, 1421, 1434, 2081, 2168, 2598, 3769
AcoI	YGGCCR	1 cut(s)	603
AcsI	RAATTY	8 cut(s)	116, 135, 1553, 2049, 2567, 2918, 3322, 3708
AcuI	CTGAAG	3 cut(s)	443, 1751, 3414
AcyI	GRCGYC	1 cut(s)	326
AfaI	GTAC	5 cut(s)	377, 1397, 1598, 1910, 2559
AfeI	AGCGCT	1 cut(s)	2905
AfiI	CCNNNNNNNGG	4 cut(s)	1348, 1375, 1482, 3785
AflIII	ACRYGT	1 cut(s)	3376
AhlI	ACTAGT	1 cut(s)	1462
AjiI	CACGTC	1 cut(s)	1392
AjnI	CCWGG	3 cut(s)	245, 2299, 2710
AjuI	GAANNNNNNNTTGG	2 cut(s)	614, 646
AloI	GAACNNNNNNTCC	2 cut(s)	3149, 3181
Alw21I	GWGCWC	1 cut(s)	2332
Alw26I	GTCTC	5 cut(s)	398, 770, 1339, 1707, 3203
Alw44I	GTGCAC	1 cut(s)	2328
AlwI	GGATC	8 cut(s)	765, 995, 1421, 1434, 2081, 2168, 2598, 3769
AlwNI	CAGNNNCTG	1 cut(s)	1436
Ama87I	CYCGRG	2 cut(s)	1874, 2334
Aor13HI	TCCGGA	1 cut(s)	1177
Aor51HI	AGCGCT	1 cut(s)	2905
ApaLI	GTGCAC	1 cut(s)	2328
ApoI	RAATTY	8 cut(s)	116, 135, 1553, 2049, 2567, 2918, 3322, 3708
ArsI	GACNNNNNNTTYG	2 cut(s)	2839, 2871
AseI	ATTAAT	1 cut(s)	2607
Asp700I	GAANNNNTTC	4 cut(s)	1115, 1356, 2237, 3624
AspS9I	GGNCC	8 cut(s)	588, 1007, 1339, 1366, 1872, 2072, 3309, 3488
AsuC2I	CCSGG	4 cut(s)	205, 1005, 2135, 3313
AsuII	TTCGAA	1 cut(s)	113
AsuNHI	GCTAGC	1 cut(s)	3049
AvaI	CYCGRG	2 cut(s)	1874, 2334
AvaII	GGWCC	4 cut(s)	588, 1007, 1366, 3309
BaeGI	GKGCMC	2 cut(s)	2326, 2332
BamHI	GGATCC	1 cut(s)	1426
BanI	GGYRCC	6 cut(s)	325, 1496, 2321, 2410, 2512, 2881
BanII	GRGCYC	1 cut(s)	3747
BarI	GAAGNNNNNNTAC	4 cut(s)	1379, 1411, 2721, 2753
BauI	CACGAG	2 cut(s)	1988, 2594
BbsI	GAAGAC	1 cut(s)	1394
Bbv12I	GWGCWC	1 cut(s)	2332
BceAI	ACGGC	2 cut(s)	31, 795
BcgI	CGANNNNNNTGC	6 cut(s)	224, 258, 379, 413, 3778, 3812
BciT130I	CCWGG	3 cut(s)	247, 2301, 2712
BciVI	GTATCC	1 cut(s)	3151
BclI	TGATCA	1 cut(s)	1885
BcnI	CCSGG	4 cut(s)	205, 1005, 2135, 3313
BcoDI	GTCTC	5 cut(s)	398, 770, 1339, 1707, 3203
BcuI	ACTAGT	1 cut(s)	1462
BfaI	CTAG	6 cut(s)	179, 1463, 1734, 2381, 3044, 3050
BfmI	CTRYAG	4 cut(s)	2391, 2865, 2950, 3071
BfoI	RGCGCY	4 cut(s)	329, 2001, 2710, 2907
BfuAI	ACCTGC	1 cut(s)	241
BfuI	GTATCC	1 cut(s)	3151
BglI	GCCNNNNNGGC	1 cut(s)	1696
BglII	AGATCT	1 cut(s)	3067
BlpI	GCTNAGC	1 cut(s)	3020
BmcAI	AGTACT	1 cut(s)	377
Bme1390I	CCNGG	7 cut(s)	205, 247, 1005, 2135, 2301, 2712, 3313
Bme18I	GGWCC	4 cut(s)	588, 1007, 1366, 3309
BmeT110I	CYCGRG	2 cut(s)	1874, 2334
BmgBI	CACGTC	1 cut(s)	1392
BmgT120I	GGNCC	8 cut(s)	588, 1007, 1339, 1366, 1872, 2072, 3309, 3488
BmrFI	CCNGG	7 cut(s)	205, 247, 1005, 2135, 2301, 2712, 3313
BmsI	GCATC	7 cut(s)	99, 1267, 2480, 2897, 3266, 3392, 3821
BmtI	GCTAGC	1 cut(s)	3053
BpiI	GAAGAC	1 cut(s)	1394
BpmI	CTGGAG	4 cut(s)	422, 731, 2848, 3269
Bpu10I	CCTNAGC	2 cut(s)	1281, 2406
Bpu1102I	GCTNAGC	1 cut(s)	3020
Bpu14I	TTCGAA	1 cut(s)	113
BpuEI	CTTGAG	7 cut(s)	1499, 2385, 2418, 2666, 2736, 3164, 3692
BpuMI	CCSGG	4 cut(s)	205, 1005, 2135, 3313
BsaAI	YACGTR	1 cut(s)	2561
BsaHI	GRCGYC	1 cut(s)	326
BsaI	GGTCTC	1 cut(s)	3203
BsaJI	CCNNGG	9 cut(s)	204, 538, 606, 1252, 1875, 2333, 2710, 2892, 3291
BsaWI	WCCGGW	1 cut(s)	1177
BsaXI	ACNNNNNCTCC	6 cut(s)	704, 734, 3242, 3272, 3767, 3797
Bsc4I	CCNNNNNNNGG	4 cut(s)	1348, 1375, 1482, 3785
Bse118I	RCCGGY	1 cut(s)	220
Bse1I	ACTGG	7 cut(s)	1430, 2244, 2391, 2514, 2677, 2831, 3694
Bse3DI	GCAATG	3 cut(s)	240, 655, 3201
BseAI	TCCGGA	1 cut(s)	1177
BseBI	CCWGG	3 cut(s)	247, 2301, 2712
BseDI	CCNNGG	9 cut(s)	204, 538, 606, 1252, 1875, 2333, 2710, 2892, 3291
BseLI	CCNNNNNNNGG	4 cut(s)	1348, 1375, 1482, 3785
BseMI	GCAATG	3 cut(s)	240, 655, 3201
BseMII	CTCAG	4 cut(s)	1077, 1650, 1917, 2933
BseNI	ACTGG	7 cut(s)	1430, 2244, 2391, 2514, 2677, 2831, 3694
BseSI	GKGCMC	2 cut(s)	2326, 2332
BseYI	CCCAGC	2 cut(s)	32, 700
BsgI	GTGCAG	1 cut(s)	533
Bsh1236I	CGCG	6 cut(s)	44, 133, 154, 323, 1371, 3320
Bsh1285I	CGRYCG	1 cut(s)	321
BshNI	GGYRCC	6 cut(s)	325, 1496, 2321, 2410, 2512, 2881
BsiEI	CGRYCG	1 cut(s)	321
BsiHKAI	GWGCWC	1 cut(s)	2332
BsiHKCI	CYCGRG	2 cut(s)	1874, 2334
BsiSI	CCGG	7 cut(s)	204, 221, 1004, 1178, 2134, 3312, 3503
BslFI	GGGAC	2 cut(s)	34, 1020
BslI	CCNNNNNNNGG	4 cut(s)	1348, 1375, 1482, 3785
BsmAI	GTCTC	5 cut(s)	398, 770, 1339, 1707, 3203
BsmBI	CGTCTC	2 cut(s)	398, 770
BsmFI	GGGAC	2 cut(s)	34, 1020
BsmI	GAATGC	1 cut(s)	3529
Bso31I	GGTCTC	1 cut(s)	3203
BsoBI	CYCGRG	2 cut(s)	1874, 2334
Bsp119I	TTCGAA	1 cut(s)	113
Bsp1286I	GDGCHC	3 cut(s)	2326, 2332, 3747
Bsp13I	TCCGGA	1 cut(s)	1177
Bsp1407I	TGTACA	1 cut(s)	1596
Bsp1720I	GCTNAGC	1 cut(s)	3020
Bsp19I	CCATGG	2 cut(s)	538, 1252
BspCNI	CTCAG	4 cut(s)	1078, 1651, 1918, 2934
BspEI	TCCGGA	1 cut(s)	1177
BspFNI	CGCG	6 cut(s)	44, 133, 154, 323, 1371, 3320
BspHI	TCATGA	1 cut(s)	3766
BspMAI	CTGCAG	1 cut(s)	3075
BspMI	ACCTGC	1 cut(s)	241
BspOI	GCTAGC	1 cut(s)	3053
BspPI	GGATC	8 cut(s)	765, 995, 1421, 1434, 2081, 2168, 2598, 3769
BspT104I	TTCGAA	1 cut(s)	113
BspT107I	GGYRCC	6 cut(s)	325, 1496, 2321, 2410, 2512, 2881
BspTNI	GGTCTC	1 cut(s)	3203
BsrBI	CCGCTC	1 cut(s)	24
BsrDI	GCAATG	3 cut(s)	240, 655, 3201
BsrFI	RCCGGY	1 cut(s)	220
BsrGI	TGTACA	1 cut(s)	1596
BsrI	ACTGG	7 cut(s)	1430, 2244, 2391, 2514, 2677, 2831, 3694
BssAI	RCCGGY	1 cut(s)	220
BssECI	CCNNGG	9 cut(s)	204, 538, 606, 1252, 1875, 2333, 2710, 2892, 3291
BssNAI	GTATAC	2 cut(s)	387, 1749
BssNI	GRCGYC	1 cut(s)	326
BssSI	CACGAG	2 cut(s)	1988, 2594
BssT1I	CCWWGG	4 cut(s)	538, 1252, 2892, 3291
Bst1107I	GTATAC	2 cut(s)	387, 1749
Bst2BI	CACGAG	2 cut(s)	1988, 2594
Bst2UI	CCWGG	3 cut(s)	247, 2301, 2712
Bst4CI	ACNGT	7 cut(s)	409, 1332, 1857, 2229, 2454, 2951, 3783
Bst6I	CTCTTC	7 cut(s)	386, 419, 617, 687, 2036, 2198, 3405
BstACI	GRCGYC	1 cut(s)	326
BstAPI	GCANNNNNTGC	1 cut(s)	3622
BstAUI	TGTACA	1 cut(s)	1596
BstBAI	YACGTR	1 cut(s)	2561
BstBI	TTCGAA	1 cut(s)	113
BstC8I	GCNNGC	7 cut(s)	222, 646, 1237, 1473, 1611, 2886, 3051
BstDEI	CTNAG	8 cut(s)	669, 1086, 1281, 1659, 1926, 2406, 2942, 3020
BstDSI	CCRYGG	2 cut(s)	538, 1252
BstEII	GGTNACC	1 cut(s)	901
BstFNI	CGCG	6 cut(s)	44, 133, 154, 323, 1371, 3320
BstH2I	RGCGCY	4 cut(s)	329, 2001, 2710, 2907
BstMAI	GTCTC	5 cut(s)	398, 770, 1339, 1707, 3203
BstMCI	CGRYCG	1 cut(s)	321
BstNI	CCWGG	3 cut(s)	247, 2301, 2712
BstNSI	RCATGY	4 cut(s)	1475, 2098, 3176, 3380
BstPI	GGTNACC	1 cut(s)	901
BstSCI	CCNGG	7 cut(s)	203, 245, 1003, 2133, 2299, 2710, 3311
BstSFI	CTRYAG	4 cut(s)	2391, 2865, 2950, 3071
BstSLI	GKGCMC	2 cut(s)	2326, 2332
BstUI	CGCG	6 cut(s)	44, 133, 154, 323, 1371, 3320
BstV2I	GAAGAC	1 cut(s)	1394
BstX2I	RGATCY	4 cut(s)	1426, 2086, 3067, 3761
BstXI	CCANNNNNNTGG	1 cut(s)	3261
BstYI	RGATCY	4 cut(s)	1426, 2086, 3067, 3761
BstZ17I	GTATAC	2 cut(s)	387, 1749
BsuI	GTATCC	1 cut(s)	3151
BtgI	CCRYGG	2 cut(s)	538, 1252
BtrI	CACGTC	1 cut(s)	1392
BtsIMutI	CAGTG	2 cut(s)	2384, 3779
BveI	ACCTGC	1 cut(s)	241
Cac8I	GCNNGC	7 cut(s)	222, 646, 1237, 1473, 1611, 2886, 3051
CaiI	CAGNNNCTG	1 cut(s)	1436
CciI	TCATGA	1 cut(s)	3766
Cfr10I	RCCGGY	1 cut(s)	220
Cfr13I	GGNCC	8 cut(s)	588, 1007, 1339, 1366, 1872, 2072, 3309, 3488
CpoI	CGGWCCG	1 cut(s)	588
CseI	GACGC	1 cut(s)	3516
Csp6I	GTAC	5 cut(s)	376, 1396, 1597, 1909, 2558
CspCI	CAANNNNNGTGG	2 cut(s)	969, 1004
CspI	CGGWCCG	1 cut(s)	588
CviQI	GTAC	5 cut(s)	376, 1396, 1597, 1909, 2558
DdeI	CTNAG	8 cut(s)	669, 1086, 1281, 1659, 1926, 2406, 2942, 3020
DinI	GGCGCC	1 cut(s)	327
EaeI	YGGCCR	1 cut(s)	603
Eam1104I	CTCTTC	7 cut(s)	386, 419, 617, 687, 2036, 2198, 3405
EarI	CTCTTC	7 cut(s)	386, 419, 617, 687, 2036, 2198, 3405
EciI	GGCGGA	1 cut(s)	220
Eco130I	CCWWGG	4 cut(s)	538, 1252, 2892, 3291
Eco147I	AGGCCT	2 cut(s)	1053, 2699
Eco24I	GRGCYC	1 cut(s)	3747
Eco31I	GGTCTC	1 cut(s)	3203
Eco32I	GATATC	1 cut(s)	1669
Eco47I	GGWCC	4 cut(s)	588, 1007, 1366, 3309
Eco47III	AGCGCT	1 cut(s)	2905
Eco57I	CTGAAG	3 cut(s)	443, 1751, 3414
Eco88I	CYCGRG	2 cut(s)	1874, 2334
Eco91I	GGTNACC	1 cut(s)	901
EcoO109I	RGGNCCY	2 cut(s)	1007, 3488
EcoO65I	GGTNACC	1 cut(s)	901
EcoRI	GAATTC	2 cut(s)	135, 3322
EcoRII	CCWGG	3 cut(s)	245, 2299, 2710
EcoRV	GATATC	1 cut(s)	1669
EcoT14I	CCWWGG	4 cut(s)	538, 1252, 2892, 3291
EcoT22I	ATGCAT	1 cut(s)	1723
EcoT38I	GRGCYC	1 cut(s)	3747
EgeI	GGCGCC	1 cut(s)	327
EheI	GGCGCC	1 cut(s)	327
ErhI	CCWWGG	4 cut(s)	538, 1252, 2892, 3291
Esp3I	CGTCTC	2 cut(s)	398, 770
FalI	AAGNNNNNCTT	2 cut(s)	3609, 3641
FaqI	GGGAC	2 cut(s)	34, 1020
FauI	CCCGC	6 cut(s)	68, 170, 295, 637, 646, 863
FauNDI	CATATG	2 cut(s)	2778, 3100
FbaI	TGATCA	1 cut(s)	1885
FblI	GTMKAC	4 cut(s)	386, 1748, 2823, 3530
FriOI	GRGCYC	1 cut(s)	3747
FspBI	CTAG	6 cut(s)	179, 1463, 1734, 2381, 3044, 3050
FspI	TGCGCA	1 cut(s)	291
GsaI	CCCAGC	2 cut(s)	36, 704
GsuI	CTGGAG	4 cut(s)	422, 731, 2848, 3269
HaeII	RGCGCY	4 cut(s)	329, 2001, 2710, 2907
HapII	CCGG	7 cut(s)	204, 221, 1004, 1178, 2134, 3312, 3503
HgaI	GACGC	1 cut(s)	3516
Hin1I	GRCGYC	1 cut(s)	326
HincII	GTYRAC	5 cut(s)	1543, 1676, 1897, 2422, 3438
HindII	GTYRAC	5 cut(s)	1543, 1676, 1897, 2422, 3438
HindIII	AAGCTT	3 cut(s)	183, 908, 3353
HpaI	GTTAAC	2 cut(s)	2422, 3438
HpaII	CCGG	7 cut(s)	204, 221, 1004, 1178, 2134, 3312, 3503
Hpy99I	CGWCG	5 cut(s)	473, 596, 1027, 1526, 3642
HpyCH4III	ACNGT	7 cut(s)	409, 1332, 1857, 2229, 2454, 2951, 3783
HpyCH4IV	ACGT	3 cut(s)	1039, 1391, 2560
HpyF3I	CTNAG	8 cut(s)	669, 1086, 1281, 1659, 1926, 2406, 2942, 3020
HpySE526I	ACGT	3 cut(s)	1039, 1391, 2560
Hsp92I	GRCGYC	1 cut(s)	326
KasI	GGCGCC	1 cut(s)	325
KflI	GGGWCCC	1 cut(s)	1007
Kpn2I	TCCGGA	1 cut(s)	1177
KroI	GCCGGC	1 cut(s)	220
KroNI	GCCGGC	1 cut(s)	222
Ksp22I	TGATCA	1 cut(s)	1885
KspAI	GTTAAC	2 cut(s)	2422, 3438
LweI	GCATC	7 cut(s)	99, 1267, 2480, 2897, 3266, 3392, 3821
MaeI	CTAG	6 cut(s)	179, 1463, 1734, 2381, 3044, 3050
MaeII	ACGT	3 cut(s)	1039, 1391, 2560
MaeIII	GTNAC	4 cut(s)	901, 2066, 2153, 3444
MbiI	CCGCTC	1 cut(s)	24
MfeI	CAATTG	1 cut(s)	1709
MflI	RGATCY	4 cut(s)	1426, 2086, 3067, 3761
MhlI	GDGCHC	3 cut(s)	2326, 2332, 3747
Mly113I	GGCGCC	1 cut(s)	326
MlyI	GAGTC	3 cut(s)	715, 890, 1892
MmeI	TCCRAC	6 cut(s)	287, 615, 724, 1644, 1649, 2421
Mph1103I	ATGCAT	1 cut(s)	1723
MroI	TCCGGA	1 cut(s)	1177
MroNI	GCCGGC	1 cut(s)	220
MroXI	GAANNNNTTC	4 cut(s)	1115, 1356, 2237, 3624
MslI	CAYNNNNRTG	4 cut(s)	1604, 1799, 2528, 3561
MspA1I	CMGCKG	2 cut(s)	36, 331
MspI	CCGG	7 cut(s)	204, 221, 1004, 1178, 2134, 3312, 3503
MspR9I	CCNGG	7 cut(s)	205, 247, 1005, 2135, 2301, 2712, 3313
MunI	CAATTG	1 cut(s)	1709
Mva1269I	GAATGC	1 cut(s)	3529
MvaI	CCWGG	3 cut(s)	247, 2301, 2712
MvnI	CGCG	6 cut(s)	44, 133, 154, 323, 1371, 3320
NaeI	GCCGGC	1 cut(s)	222
NarI	GGCGCC	1 cut(s)	326
NciI	CCSGG	4 cut(s)	205, 1005, 2135, 3313
NcoI	CCATGG	2 cut(s)	538, 1252
NdeI	CATATG	2 cut(s)	2778, 3100
NgoMIV	GCCGGC	1 cut(s)	220
NheI	GCTAGC	1 cut(s)	3049
NmeAIII	GCCGAG	1 cut(s)	631
NmuCI	GTSAC	1 cut(s)	3444
NsbI	TGCGCA	1 cut(s)	291
NsiI	ATGCAT	1 cut(s)	1723
NspI	RCATGY	4 cut(s)	1475, 2098, 3176, 3380
NspV	TTCGAA	1 cut(s)	113
PaeI	GCATGC	1 cut(s)	1475
PagI	TCATGA	1 cut(s)	3766
PceI	AGGCCT	2 cut(s)	1053, 2699
PciI	ACATGT	1 cut(s)	3376
PcsI	WCGNNNNNNNCGW	4 cut(s)	119, 386, 413, 3231
PctI	GAATGC	1 cut(s)	3529
PdiI	GCCGGC	1 cut(s)	222
PdmI	GAANNNNTTC	4 cut(s)	1115, 1356, 2237, 3624
PflMI	CCANNNNNTGG	1 cut(s)	3785
PfoI	TCCNGGA	2 cut(s)	1003, 3311
PleI	GAGTC	3 cut(s)	714, 890, 1892
PluTI	GGCGCC	1 cut(s)	329
PpsI	GAGTC	3 cut(s)	714, 890, 1892
Ppu21I	YACGTR	1 cut(s)	2561
PpuMI	RGGWCCY	1 cut(s)	1007
PscI	ACATGT	1 cut(s)	3376
PshBI	ATTAAT	1 cut(s)	2607
PsiI	TTATAA	1 cut(s)	3609
Psp5II	RGGWCCY	1 cut(s)	1007
Psp6I	CCWGG	3 cut(s)	245, 2299, 2710
PspEI	GGTNACC	1 cut(s)	901
PspFI	CCCAGC	2 cut(s)	32, 700
PspGI	CCWGG	3 cut(s)	245, 2299, 2710
PspPI	GGNCC	8 cut(s)	588, 1007, 1339, 1366, 1872, 2072, 3309, 3488
PspPPI	RGGWCCY	1 cut(s)	1007
PstI	CTGCAG	1 cut(s)	3075
PstNI	CAGNNNCTG	1 cut(s)	1436
PsuI	RGATCY	4 cut(s)	1426, 2086, 3067, 3761
PvuII	CAGCTG	1 cut(s)	36
RsaI	GTAC	5 cut(s)	377, 1397, 1598, 1910, 2559
RsaNI	GTAC	5 cut(s)	376, 1396, 1597, 1909, 2558
RseI	CAYNNNNRTG	4 cut(s)	1604, 1799, 2528, 3561
Rsr2I	CGGWCCG	1 cut(s)	588
RsrII	CGGWCCG	1 cut(s)	588
Sau96I	GGNCC	8 cut(s)	588, 1007, 1339, 1366, 1872, 2072, 3309, 3488
ScaI	AGTACT	1 cut(s)	377
SchI	GAGTC	3 cut(s)	715, 890, 1892
ScrFI	CCNGG	7 cut(s)	205, 247, 1005, 2135, 2301, 2712, 3313
SduI	GDGCHC	3 cut(s)	2326, 2332, 3747
SfaNI	GCATC	7 cut(s)	99, 1267, 2480, 2897, 3266, 3392, 3821
SfcI	CTRYAG	4 cut(s)	2391, 2865, 2950, 3071
SfoI	GGCGCC	1 cut(s)	327
SfuI	TTCGAA	1 cut(s)	113
SinI	GGWCC	4 cut(s)	588, 1007, 1366, 3309
SmiMI	CAYNNNNRTG	4 cut(s)	1604, 1799, 2528, 3561
SmlI	CTYRAG	7 cut(s)	1514, 2400, 2433, 2681, 2751, 3143, 3671
SmoI	CTYRAG	7 cut(s)	1514, 2400, 2433, 2681, 2751, 3143, 3671
SpeI	ACTAGT	1 cut(s)	1462
SphI	GCATGC	1 cut(s)	1475
SseBI	AGGCCT	2 cut(s)	1053, 2699
SspDI	GGCGCC	1 cut(s)	325
SspI	AATATT	2 cut(s)	1535, 2120
SspMI	CTAG	6 cut(s)	179, 1463, 1734, 2381, 3044, 3050
StuI	AGGCCT	2 cut(s)	1053, 2699
StyD4I	CCNGG	7 cut(s)	203, 245, 1003, 2133, 2299, 2710, 3311
StyI	CCWWGG	4 cut(s)	538, 1252, 2892, 3291
TaaI	ACNGT	7 cut(s)	409, 1332, 1857, 2229, 2454, 2951, 3783
TaiI	ACGT	3 cut(s)	1042, 1394, 2563
TatI	WGTACW	2 cut(s)	375, 1596
TauI	GCSGC	6 cut(s)	80, 90, 326, 331, 479, 785
TscAI	CASTG	2 cut(s)	2391, 3786
TseFI	GTSAC	1 cut(s)	3444
Tsp45I	GTSAC	1 cut(s)	3444
TspGWI	ACGGA	5 cut(s)	1040, 1438, 1661, 2022, 3478
TspRI	CASTG	2 cut(s)	2391, 3786
Van91I	CCANNNNNTGG	1 cut(s)	3785
VneI	GTGCAC	1 cut(s)	2328
VpaK11BI	GGWCC	4 cut(s)	588, 1007, 1366, 3309
VspI	ATTAAT	1 cut(s)	2607
XapI	RAATTY	8 cut(s)	116, 135, 1553, 2049, 2567, 2918, 3322, 3708
XbaI	TCTAGA	1 cut(s)	178
XceI	RCATGY	4 cut(s)	1475, 2098, 3176, 3380
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1250, 3177
XmiI	GTMKAC	4 cut(s)	386, 1748, 2823, 3530
XmnI	GAANNNNTTC	4 cut(s)	1115, 1356, 2237, 3624
XspI	CTAG	6 cut(s)	179, 1463, 1734, 2381, 3044, 3050
ZrmI	AGTACT	1 cut(s)	377
Zsp2I	ATGCAT	1 cut(s)	1723

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.