Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0013690

Physical Location & Seq

Genomic Coordinates Reverse (-)

32067852 .. 32071212

Gene Structure 3361 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1244700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2154 bp

ATGGAGCAAGAAGACGACGCCGTCACGGAGAGCAAAGCAGACGGAGGAGGAGGCGGTGGTGGTAGTGGTGGAGCAGGGAGCGGTATTAGCATTGCAGAGAGGAGGGCAGCCAAGTGTGGATTCAATGCGGAGAGGATCAACACGGTGCGTGTCAGCACAACCAACCCCTTGAATTCTCCGACGGCGAGGTCCCCGTGCCTTACTATTCCGTCTGGTATCAGCCCCACGGCTCTGCTCGACTCCCCTGTGATGGTCCCCAATTCTCAGGCGCTTCCATCTCCCACCACCGGAACTTTTCCATTGCGTCCTCCCAATGATAACAGCTTGCTGCTGAAATCAGGAACTCGTGAAGATGATGGTTACAGGCCGGCCTATTCTAGTTTTGAAAATCAGGGATCTACTGTTGACTACCAAGCTCTTGTTTTGGAGCAACAACCAATGGAGTTTCAATTCCCGATGGAGTTTTCCAAAGAAGCTAATGCAACGGACTATGCTGTGGATCCGTCCACTGATGTTAGAAACATAAACAGTGGTGTGATTATGAATGCCAACAGCGCAGACTTGCAAATGTCTCATTCCAGCGCAGCTAGTGATCAAAGCTCTCTTCCCAAAGAAGCGGTTCATGGGGAGGACATGGGGACCCATCAGTTTTCAGAAGGAGAGCATAAAGGGTCATACTCATCTGCAGGAATGGTAAGGACCTCAGAACACGGATACAATTGGAGGAAGTATGGGCAGAAACAGGTAAAAGGTAGTGAATATCCAAGGAGTTATTACAAATGCACGCATCCAAATTGTCAGGTGAAGAAAAAGGTGGAACGATCCTATGATGGTCAAATAACAGAAATCATCTACAAGGGAGCTCCTCATAATCATGCAAAACCTCAGCCTAACCGTAGAGCTGGAGCATCGGTTGGACCATCATTTTCTTATGATGACATGTCAGAGATGGGTGAAGGTAGTAGGGCTGCTGTCAAAGTTGAAGGTGGGCCAGTTTGGACAGATATTCAGTCCTGTAAGGATAGGAAAGTTGGCTACGATGGGAGGCCTGACGGTCTGGAAAGGACATCCTCAGCATCTGTTGTTACCGAGCTTTCTGATCCATTTTCAACTACCCAAGGAAAGTCCATGAATATCTTTGAATCAGCCGAAACTCCAGAGCTTTCATCCACACTTGCTAGTCATGATGATGATGATCGGGCCACCCAAGGAAGCATATCACTTGGAGATGATGCAGATGATGAAGAATCTGATTCGAAAAGAAGAAAGAAGGAGAGCTGCATGATTGAAACAAATTTTGCATCCAGGGATGTCCGCAAACCAAGAGTAGTTGTCCAAATTGAGAGCGAAATTGACATACTTGATGATGGCTATCGCTGGCGGAAGTATGGGCAAAAAGTTGTCAAAGGAAATCCAAACCCCAGGAGCTATTACAAGTGCACAAGTGCAGGATGTTCAGTAAGGAAGCACGTGGAAAGGGCCTCCCACGATCTGAAATTCGTAATTACAGCGTATGAGGGAAAACACAACCACGAAGTGCCTGCAGCGAGAAACAGTAATCACATGAATTCAAGTAGCGGTAATGGACTCCCATCTACTGCCAATGCTCAGCCAGCTCTGGCATTACCTAGAAATACCAATATTCCAAAGCCTGAGACACAAGTACAAGATCTTGCACCTCATTTTGATAGAAAACCGGAATTCCATGACAAATACATGAGGACTAGTTTTCTCGGGAATTTCAGTAATGACATGAAATTTGGAGCTCCCTCCCTTTACCAAATGAAATATCCTCCTCCGCAGAATACCATGCTGTATGCCTCATTCGGAATGCATGCTAATCACAATGTGGCTCATCAGGCTGGTTCTGTTGTCCCAGACTTCCCAATTTCACTGCCTATGAGCCTTCCGCCATCCGGTAACCTCCCTCTCGCCAGTTATGATTTCAACAACGGAAAATCAGTTTGTCCAGAACAGCCTTATTTTTCCAGACAGCAGCTGCAGGAGATCGATGTAAGATTCCTACAGCCTAAACAAGAGCACAAGGATGATAACCCTTATGATGCCCGCTTCCCAACAATGGATCAGTCAAGTGCAGCATTGTCGTCGCCATCGCTATTCTATCACAGGGTCATGGGAGGTTATCCGTCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000578 GO:0003006 GO:0003674 GO:0003700 GO:0006355 GO:0007163 GO:0007275 GO:0007389 GO:0008150 GO:0009555 GO:0009790 GO:0009791 GO:0009793 GO:0009798 GO:0009880 GO:0009889 GO:0009942 GO:0009987 GO:0010154 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022414 GO:0030010 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0048229 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

WRKY

Protein Analysis

718

Amino Acids

78.54

Weight (kDa)

5.99

Isoelectric Point (pI)

54.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	237 - 292	1.2e-22	WRKY DNA -binding domain
WRKY	PF03106	455 - 512	3.7e-25	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013690)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g00500
malus_domestica	MD04G1244700.v1.1 MD12G1260600.v1.1
prunus_persica	Prupe.6G361300_v2.0.a1
pyrus_communis	pycom04g21550 pycom12g23860
rosa_chinensis	RchiOBHm_Chr3g0447881
rosa_laevigata	RLG00000025964
rosa_multiflora	Rmu_sc0005969.1_g000001 Rmu_sc0020869.1_g000002 Rmu_sc0033808.1_g000003
rosa_roxburghii	Rroxscaffold_6G00426080
rosa_rugosa	Rorug02G0606800
rosa_samantha	Rh3AG006000 Rh3BG005700 Rh3CG005900 Rh3DG006200
rosa_wichuraiana	Rw3G000460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	20, 187
AccBSI	CCGCTC	1 cut(s)	81
AclWI	GGATC	7 cut(s)	143, 403, 494, 507, 816, 1094, 2091
AcsI	RAATTY	7 cut(s)	172, 1294, 1496, 1567, 1700, 1738, 1757
AcvI	CACGTG	1 cut(s)	1471
AcyI	GRCGYC	1 cut(s)	18
AdeI	CACNNNGTG	2 cut(s)	1537, 1849
AfaI	GTAC	1 cut(s)	1665
AfiI	CCNNNNNNNGG	4 cut(s)	250, 287, 1916, 2080
AflIII	ACRYGT	1 cut(s)	939
AhlI	ACTAGT	1 cut(s)	1724
AjnI	CCWGG	2 cut(s)	1304, 1421
AjuI	GAANNNNNNNTTGG	2 cut(s)	104, 136
Alw21I	GWGCWC	4 cut(s)	865, 1442, 1768, 2043
Alw26I	GTCTC	2 cut(s)	576, 1649
Alw44I	GTGCAC	1 cut(s)	1438
AlwI	GGATC	7 cut(s)	143, 403, 494, 507, 816, 1094, 2091
AlwNI	CAGNNNCTG	2 cut(s)	1079, 1999
Ama87I	CYCGRG	1 cut(s)	1733
AoxI	GGCC	6 cut(s)	365, 369, 989, 1046, 1200, 1479
ApaLI	GTGCAC	1 cut(s)	1438
ApeKI	GCWGC	9 cut(s)	107, 328, 584, 968, 1278, 1544, 1996, 1999, 2096
ApoI	RAATTY	7 cut(s)	172, 1294, 1496, 1567, 1700, 1738, 1757
ArsI	GACNNNNNNTTYG	2 cut(s)	1742, 1774
Asp700I	GAANNNNTTC	1 cut(s)	618
AspLEI	GCGC	3 cut(s)	271, 557, 584
AspS9I	GGNCC	8 cut(s)	189, 253, 639, 699, 917, 989, 1200, 1479
AsuHPI	GGTGA	2 cut(s)	814, 965
AsuII	TTCGAA	1 cut(s)	1256
AvaI	CYCGRG	1 cut(s)	1733
AvaII	GGWCC	5 cut(s)	189, 253, 639, 699, 917
BaeGI	GKGCMC	1 cut(s)	1442
BamHI	GGATCC	1 cut(s)	499
BanII	GRGCYC	2 cut(s)	865, 1768
BauI	CACGAG	1 cut(s)	345
BbrPI	CACGTG	1 cut(s)	1471
BbsI	GAAGAC	1 cut(s)	18
Bbv12I	GWGCWC	4 cut(s)	865, 1442, 1768, 2043
BbvCI	CCTCAGC	2 cut(s)	885, 1072
BbvI	GCAGC	9 cut(s)	119, 315, 596, 955, 1265, 1556, 1986, 2008, 2108
BceAI	ACGGC	3 cut(s)	5, 198, 243
BcgI	CGANNNNNNTGC	6 cut(s)	1523, 1557, 2085, 2088, 2119, 2122
BciT130I	CCWGG	2 cut(s)	1306, 1423
BciVI	GTATCC	1 cut(s)	707
BclI	TGATCA	1 cut(s)	592
BcoDI	GTCTC	2 cut(s)	576, 1649
BcuI	ACTAGT	1 cut(s)	1724
BfaI	CTAG	5 cut(s)	378, 588, 1179, 1629, 1725
BfmI	CTRYAG	4 cut(s)	684, 1542, 2000, 2024
BfoI	RGCGCY	1 cut(s)	272
BfuI	GTATCC	1 cut(s)	707
BglII	AGATCT	1 cut(s)	1669
BisI	GCNGC	9 cut(s)	108, 329, 585, 969, 1279, 1545, 1997, 2000, 2097
BlpI	GCTNAGC	1 cut(s)	1608
BlsI	GCNGC	9 cut(s)	109, 330, 586, 970, 1280, 1546, 1998, 2001, 2098
Bme1390I	CCNGG	2 cut(s)	1306, 1423
Bme18I	GGWCC	5 cut(s)	189, 253, 639, 699, 917
BmeT110I	CYCGRG	1 cut(s)	1733
BmgT120I	GGNCC	8 cut(s)	189, 253, 639, 699, 917, 989, 1200, 1479
BmiI	GGNNCC	5 cut(s)	191, 255, 501, 640, 641
BmrFI	CCNGG	2 cut(s)	1306, 1423
BmsI	GCATC	6 cut(s)	796, 917, 1085, 1222, 1310, 2053
BpiI	GAAGAC	1 cut(s)	18
BpmI	CTGGAG	2 cut(s)	924, 1140
Bpu10I	CCTNAGC	2 cut(s)	885, 1072
Bpu1102I	GCTNAGC	1 cut(s)	1608
Bpu14I	TTCGAA	1 cut(s)	1256
Bsa29I	ATCGAT	1 cut(s)	2010
BsaAI	YACGTR	1 cut(s)	1471
BsaBI	GATNNNNATC	2 cut(s)	1194, 1371
BsaHI	GRCGYC	1 cut(s)	18
BsaJI	CCNNGG	6 cut(s)	225, 764, 1117, 1207, 1305, 1421
BsaWI	WCCGGW	3 cut(s)	287, 1696, 1916
BsaXI	ACNNNNNCTCC	2 cut(s)	897, 927
Bsc4I	CCNNNNNNNGG	4 cut(s)	250, 287, 1916, 2080
Bse118I	RCCGGY	1 cut(s)	367
Bse1I	ACTGG	2 cut(s)	992, 1935
Bse3DI	GCAATG	2 cut(s)	90, 299
Bse8I	GATNNNNATC	2 cut(s)	1194, 1371
BseBI	CCWGG	2 cut(s)	1306, 1423
BseCI	ATCGAT	1 cut(s)	2010
BseDI	CCNNGG	6 cut(s)	225, 764, 1117, 1207, 1305, 1421
BseGI	GGATG	8 cut(s)	787, 1067, 1166, 1301, 1315, 1457, 1913, 2053
BseJI	GATNNNNATC	2 cut(s)	1194, 1371
BseLI	CCNNNNNNNGG	4 cut(s)	250, 287, 1916, 2080
BseMI	GCAATG	2 cut(s)	90, 299
BseMII	CTCAG	6 cut(s)	278, 717, 899, 1086, 1622, 1644
BseNI	ACTGG	2 cut(s)	992, 1935
BseRI	GAGGAG	5 cut(s)	60, 63, 115, 855, 1785
BseSI	GKGCMC	1 cut(s)	1442
BseXI	GCAGC	9 cut(s)	119, 315, 596, 955, 1265, 1556, 1986, 2008, 2108
BsgI	GTGCAG	2 cut(s)	1467, 2115
BshFI	GGCC	6 cut(s)	367, 371, 991, 1048, 1202, 1481
BshVI	ATCGAT	1 cut(s)	2010
BsiHKAI	GWGCWC	4 cut(s)	865, 1442, 1768, 2043
BsiHKCI	CYCGRG	1 cut(s)	1733
BsiSI	CCGG	4 cut(s)	288, 368, 1697, 1917
BslFI	GGGAC	4 cut(s)	175, 239, 652, 1859
BslI	CCNNNNNNNGG	4 cut(s)	250, 287, 1916, 2080
BsmAI	GTCTC	2 cut(s)	576, 1649
BsmFI	GGGAC	4 cut(s)	175, 239, 652, 1859
BsmI	GAATGC	2 cut(s)	550, 1836
BsnI	GGCC	6 cut(s)	367, 371, 991, 1048, 1202, 1481
BsoBI	CYCGRG	1 cut(s)	1733
Bsp119I	TTCGAA	1 cut(s)	1256
Bsp1286I	GDGCHC	4 cut(s)	865, 1442, 1768, 2043
Bsp1720I	GCTNAGC	1 cut(s)	1608
BspANI	GGCC	6 cut(s)	367, 371, 991, 1048, 1202, 1481
BspCNI	CTCAG	6 cut(s)	277, 716, 898, 1085, 1621, 1645
BspDI	ATCGAT	1 cut(s)	2010
BspHI	TCATGA	1 cut(s)	1183
BspLI	GGNNCC	5 cut(s)	191, 255, 501, 640, 641
BspMAI	CTGCAG	3 cut(s)	688, 1546, 2004
BspPI	GGATC	7 cut(s)	143, 403, 494, 507, 816, 1094, 2091
BspT104I	TTCGAA	1 cut(s)	1256
BsrBI	CCGCTC	1 cut(s)	81
BsrDI	GCAATG	2 cut(s)	90, 299
BsrFI	RCCGGY	1 cut(s)	367
BsrI	ACTGG	2 cut(s)	992, 1935
BssAI	RCCGGY	1 cut(s)	367
BssECI	CCNNGG	6 cut(s)	225, 764, 1117, 1207, 1305, 1421
BssNI	GRCGYC	1 cut(s)	18
BssSI	CACGAG	1 cut(s)	345
BssT1I	CCWWGG	3 cut(s)	764, 1117, 1207
Bst2BI	CACGAG	1 cut(s)	345
Bst2UI	CCWGG	2 cut(s)	1306, 1423
Bst4CI	ACNGT	6 cut(s)	145, 403, 530, 896, 1055, 1556
Bst6I	CTCTTC	1 cut(s)	609
BstACI	GRCGYC	1 cut(s)	18
BstBAI	YACGTR	1 cut(s)	1471
BstBI	TTCGAA	1 cut(s)	1256
BstC8I	GCNNGC	8 cut(s)	326, 369, 785, 1379, 1542, 1614, 1836, 2068
BstDEI	CTNAG	6 cut(s)	264, 703, 885, 1072, 1608, 1653
BstDSI	CCRYGG	1 cut(s)	225
BstEII	GGTNACC	1 cut(s)	1919
BstF5I	GGATG	8 cut(s)	787, 1067, 1166, 1301, 1315, 1457, 1913, 2053
BstH2I	RGCGCY	1 cut(s)	272
BstHHI	GCGC	3 cut(s)	271, 557, 584
BstMAI	GTCTC	2 cut(s)	576, 1649
BstMWI	GCNNNNNNNGC	4 cut(s)	87, 554, 1613, 1859
BstNI	CCWGG	2 cut(s)	1306, 1423
BstNSI	RCATGY	2 cut(s)	943, 1838
BstPI	GGTNACC	1 cut(s)	1919
BstSCI	CCNGG	2 cut(s)	1304, 1421
BstSFI	CTRYAG	4 cut(s)	684, 1542, 2000, 2024
BstSLI	GKGCMC	1 cut(s)	1442
BstV1I	GCAGC	9 cut(s)	119, 315, 596, 955, 1265, 1556, 1986, 2008, 2108
BstV2I	GAAGAC	1 cut(s)	18
BstX2I	RGATCY	3 cut(s)	395, 499, 1669
BstYI	RGATCY	3 cut(s)	395, 499, 1669
Bsu15I	ATCGAT	1 cut(s)	2010
BsuI	GTATCC	1 cut(s)	707
BsuRI	GGCC	6 cut(s)	367, 371, 991, 1048, 1202, 1481
BsuTUI	ATCGAT	1 cut(s)	2010
BtgI	CCRYGG	1 cut(s)	225
BtgZI	GCGATG	1 cut(s)	2097
BtsCI	GGATG	8 cut(s)	787, 1067, 1166, 1301, 1315, 1457, 1913, 2053
BtsI	GCAGTG	1 cut(s)	1892
BtsIMutI	CAGTG	3 cut(s)	507, 535, 1892
Cac8I	GCNNGC	8 cut(s)	326, 369, 785, 1379, 1542, 1614, 1836, 2068
CaiI	CAGNNNCTG	2 cut(s)	1079, 1999
CciI	TCATGA	1 cut(s)	1183
CfoI	GCGC	3 cut(s)	271, 557, 584
Cfr10I	RCCGGY	1 cut(s)	367
Cfr13I	GGNCC	8 cut(s)	189, 253, 639, 699, 917, 989, 1200, 1479
ClaI	ATCGAT	1 cut(s)	2010
CseI	GACGC	2 cut(s)	26, 293
Csp6I	GTAC	1 cut(s)	1664
CviQI	GTAC	1 cut(s)	1664
DdeI	CTNAG	6 cut(s)	264, 703, 885, 1072, 1608, 1653
DraIII	CACNNNGTG	2 cut(s)	1537, 1849
DrdI	GACNNNNNNGTC	2 cut(s)	20, 187
DseDI	GACNNNNNNGTC	2 cut(s)	20, 187
Eam1104I	CTCTTC	1 cut(s)	609
EarI	CTCTTC	1 cut(s)	609
EciI	GGCGGA	2 cut(s)	1396, 1899
Ecl136II	GAGCTC	2 cut(s)	863, 1766
Eco130I	CCWWGG	3 cut(s)	764, 1117, 1207
Eco147I	AGGCCT	1 cut(s)	1048
Eco24I	GRGCYC	2 cut(s)	865, 1768
Eco47I	GGWCC	5 cut(s)	189, 253, 639, 699, 917
Eco53kI	GAGCTC	2 cut(s)	863, 1766
Eco72I	CACGTG	1 cut(s)	1471
Eco88I	CYCGRG	1 cut(s)	1733
Eco91I	GGTNACC	1 cut(s)	1919
EcoICRI	GAGCTC	2 cut(s)	863, 1766
EcoO109I	RGGNCCY	4 cut(s)	189, 639, 699, 1479
EcoO65I	GGTNACC	1 cut(s)	1919
EcoRI	GAATTC	3 cut(s)	172, 1567, 1700
EcoRII	CCWGG	2 cut(s)	1304, 1421
EcoT14I	CCWWGG	3 cut(s)	764, 1117, 1207
EcoT22I	ATGCAT	1 cut(s)	1836
EcoT38I	GRGCYC	2 cut(s)	865, 1768
ErhI	CCWWGG	3 cut(s)	764, 1117, 1207
FaqI	GGGAC	4 cut(s)	175, 239, 652, 1859
FauI	CCCGC	1 cut(s)	2075
FbaI	TGATCA	1 cut(s)	592
Fnu4HI	GCNGC	9 cut(s)	108, 329, 585, 969, 1279, 1545, 1997, 2000, 2097
FokI	GGATG	8 cut(s)	774, 1054, 1153, 1288, 1322, 1464, 1900, 2060
FriOI	GRGCYC	2 cut(s)	865, 1768
FseI	GGCCGGCC	1 cut(s)	371
Fsp4HI	GCNGC	9 cut(s)	108, 329, 585, 969, 1279, 1545, 1997, 2000, 2097
FspBI	CTAG	5 cut(s)	378, 588, 1179, 1629, 1725
GlaI	GCGC	3 cut(s)	270, 556, 583
GluI	GCNGC	9 cut(s)	108, 329, 585, 969, 1279, 1545, 1997, 2000, 2097
GsuI	CTGGAG	2 cut(s)	924, 1140
HaeII	RGCGCY	1 cut(s)	272
HaeIII	GGCC	6 cut(s)	367, 371, 991, 1048, 1202, 1481
HapII	CCGG	4 cut(s)	288, 368, 1697, 1917
HgaI	GACGC	2 cut(s)	26, 293
HhaI	GCGC	3 cut(s)	271, 557, 584
Hin1I	GRCGYC	1 cut(s)	18
Hin6I	GCGC	3 cut(s)	269, 555, 582
HinP1I	GCGC	3 cut(s)	269, 555, 582
HincII	GTYRAC	1 cut(s)	406
HindII	GTYRAC	1 cut(s)	406
HinfI	GANTC	7 cut(s)	120, 239, 1142, 1247, 1253, 1587, 2019
HpaII	CCGG	4 cut(s)	288, 368, 1697, 1917
HphI	GGTGA	2 cut(s)	814, 965
Hpy166II	GTNNAC	3 cut(s)	406, 507, 1440
Hpy188I	TCNGA	8 cut(s)	180, 655, 706, 946, 1099, 1252, 1494, 1829
Hpy188III	TCNNGA	9 cut(s)	339, 347, 454, 1058, 1157, 1184, 1735, 1970, 1989
Hpy8I	GTNNAC	3 cut(s)	406, 507, 1440
Hpy99I	CGWCG	3 cut(s)	20, 184, 2110
HpyAV	CCTTC	5 cut(s)	650, 950, 977, 1264, 1916
HpyCH4III	ACNGT	6 cut(s)	145, 403, 530, 896, 1055, 1556
HpyCH4IV	ACGT	1 cut(s)	1470
HpyF10VI	GCNNNNNNNGC	4 cut(s)	87, 554, 1613, 1859
HpyF3I	CTNAG	6 cut(s)	264, 703, 885, 1072, 1608, 1653
HpySE526I	ACGT	1 cut(s)	1470
Hsp92I	GRCGYC	1 cut(s)	18
HspAI	GCGC	3 cut(s)	269, 555, 582
KflI	GGGWCCC	1 cut(s)	639
KroI	GCCGGC	1 cut(s)	367
KroNI	GCCGGC	1 cut(s)	369
Ksp22I	TGATCA	1 cut(s)	592
Lsp1109I	GCAGC	9 cut(s)	119, 315, 596, 955, 1265, 1556, 1986, 2008, 2108
LweI	GCATC	6 cut(s)	796, 917, 1085, 1222, 1310, 2053
MaeI	CTAG	5 cut(s)	378, 588, 1179, 1629, 1725
MaeII	ACGT	1 cut(s)	1470
MaeIII	GTNAC	4 cut(s)	22, 359, 1084, 1919
MbiI	CCGCTC	1 cut(s)	81
MboII	GAAGA	6 cut(s)	23, 362, 596, 817, 1256, 1275
MfeI	CAATTG	1 cut(s)	718
MflI	RGATCY	3 cut(s)	395, 499, 1669
MhlI	GDGCHC	4 cut(s)	865, 1442, 1768, 2043
MlyI	GAGTC	2 cut(s)	233, 1581
MmeI	TCCRAC	2 cut(s)	203, 895
Mph1103I	ATGCAT	1 cut(s)	1836
MroNI	GCCGGC	1 cut(s)	367
MroXI	GAANNNNTTC	1 cut(s)	618
MslI	CAYNNNNRTG	3 cut(s)	873, 1188, 2046
MspA1I	CMGCKG	1 cut(s)	1999
MspI	CCGG	4 cut(s)	288, 368, 1697, 1917
MspR9I	CCNGG	2 cut(s)	1306, 1423
MunI	CAATTG	1 cut(s)	718
Mva1269I	GAATGC	2 cut(s)	550, 1836
MvaI	CCWGG	2 cut(s)	1306, 1423
MwoI	GCNNNNNNNGC	4 cut(s)	87, 554, 1613, 1859
NaeI	GCCGGC	1 cut(s)	369
NgoMIV	GCCGGC	1 cut(s)	367
NlaIV	GGNNCC	5 cut(s)	191, 255, 501, 640, 641
NmuCI	GTSAC	1 cut(s)	22
NsiI	ATGCAT	1 cut(s)	1836
NspI	RCATGY	2 cut(s)	943, 1838
NspV	TTCGAA	1 cut(s)	1256
PaeI	GCATGC	1 cut(s)	1838
PagI	TCATGA	1 cut(s)	1183
PceI	AGGCCT	1 cut(s)	1048
PciI	ACATGT	1 cut(s)	939
PctI	GAATGC	2 cut(s)	550, 1836
PdiI	GCCGGC	1 cut(s)	369
PdmI	GAANNNNTTC	1 cut(s)	618
PfeI	GAWTC	5 cut(s)	120, 1142, 1247, 1253, 2019
PflFI	GACNNNGTC	1 cut(s)	20
PkrI	GCNGC	9 cut(s)	109, 330, 586, 970, 1280, 1546, 1998, 2001, 2098
PleI	GAGTC	2 cut(s)	233, 1581
PmaCI	CACGTG	1 cut(s)	1471
PmlI	CACGTG	1 cut(s)	1471
PpsI	GAGTC	2 cut(s)	233, 1581
Ppu21I	YACGTR	1 cut(s)	1471
PpuMI	RGGWCCY	3 cut(s)	189, 639, 699
PscI	ACATGT	1 cut(s)	939
Psp124BI	GAGCTC	2 cut(s)	865, 1768
Psp5II	RGGWCCY	3 cut(s)	189, 639, 699
Psp6I	CCWGG	2 cut(s)	1304, 1421
PspCI	CACGTG	1 cut(s)	1471
PspEI	GGTNACC	1 cut(s)	1919
PspGI	CCWGG	2 cut(s)	1304, 1421
PspN4I	GGNNCC	5 cut(s)	191, 255, 501, 640, 641
PspPI	GGNCC	8 cut(s)	189, 253, 639, 699, 917, 989, 1200, 1479
PspPPI	RGGWCCY	3 cut(s)	189, 639, 699
PstI	CTGCAG	3 cut(s)	688, 1546, 2004
PstNI	CAGNNNCTG	2 cut(s)	1079, 1999
PsuI	RGATCY	3 cut(s)	395, 499, 1669
PsyI	GACNNNGTC	1 cut(s)	20
PvuII	CAGCTG	1 cut(s)	1999
RigI	GGCCGGCC	1 cut(s)	371
RsaI	GTAC	1 cut(s)	1665
RsaNI	GTAC	1 cut(s)	1664
RseI	CAYNNNNRTG	3 cut(s)	873, 1188, 2046
SacI	GAGCTC	2 cut(s)	865, 1768
SatI	GCNGC	9 cut(s)	108, 329, 585, 969, 1279, 1545, 1997, 2000, 2097
Sau96I	GGNCC	8 cut(s)	189, 253, 639, 699, 917, 989, 1200, 1479
SchI	GAGTC	2 cut(s)	233, 1581
ScrFI	CCNGG	2 cut(s)	1306, 1423
SduI	GDGCHC	4 cut(s)	865, 1442, 1768, 2043
SfaNI	GCATC	6 cut(s)	796, 917, 1085, 1222, 1310, 2053
SfcI	CTRYAG	4 cut(s)	684, 1542, 2000, 2024
SfuI	TTCGAA	1 cut(s)	1256
SinI	GGWCC	5 cut(s)	189, 253, 639, 699, 917
SmiMI	CAYNNNNRTG	3 cut(s)	873, 1188, 2046
SpeI	ACTAGT	1 cut(s)	1724
SphI	GCATGC	1 cut(s)	1838
SseBI	AGGCCT	1 cut(s)	1048
SspI	AATATT	1 cut(s)	1642
SspMI	CTAG	5 cut(s)	378, 588, 1179, 1629, 1725
SstI	GAGCTC	2 cut(s)	865, 1768
StuI	AGGCCT	1 cut(s)	1048
StyD4I	CCNGG	2 cut(s)	1304, 1421
StyI	CCWWGG	3 cut(s)	764, 1117, 1207
TaaI	ACNGT	6 cut(s)	145, 403, 530, 896, 1055, 1556
TaiI	ACGT	1 cut(s)	1473
TaqI	TCGA	3 cut(s)	237, 1256, 2010
TatI	WGTACW	1 cut(s)	1663
TfiI	GAWTC	5 cut(s)	120, 1142, 1247, 1253, 2019
TscAI	CASTG	3 cut(s)	514, 535, 1899
TseFI	GTSAC	1 cut(s)	22
TseI	GCWGC	9 cut(s)	107, 328, 584, 968, 1278, 1544, 1996, 1999, 2096
Tsp45I	GTSAC	1 cut(s)	22
TspDTI	ATGAA	8 cut(s)	557, 611, 1145, 1155, 1257, 1580, 1769, 1799
TspGWI	ACGGA	8 cut(s)	41, 57, 198, 492, 500, 726, 1968, 2136
TspRI	CASTG	3 cut(s)	514, 535, 1899
Tth111I	GACNNNGTC	1 cut(s)	20
VneI	GTGCAC	1 cut(s)	1438
VpaK11BI	GGWCC	5 cut(s)	189, 253, 639, 699, 917
XapI	RAATTY	7 cut(s)	172, 1294, 1496, 1567, 1700, 1738, 1757
XceI	RCATGY	2 cut(s)	943, 1838
XmnI	GAANNNNTTC	1 cut(s)	618
XspI	CTAG	5 cut(s)	378, 588, 1179, 1629, 1725
Zsp2I	ATGCAT	1 cut(s)	1836

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.