Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0013690

Physical Location & Seq

Genomic Coordinates Forward (+)

46950208 .. 46954599

Gene Structure 4392 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00426080.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2202 bp

ATGGAGCAAGCAAACGATGTGGTCGTTGCAGAGAACAAAGCCAAATATGGAGGTGGTGGCGGGAGCATTAGCATAGCCGAGAGGAGGGCAGCAAAGTGTGGATTCAATGCGGAGAGGATCAATACGGCGCGTTTCAGGAGGACCAACACCAGCCCTCTGCCTTCTCCGGTGGAGAGGTCTCCTTGCCACCTCACTATTCCTCCCGGCATCAGTCCCACCGCTTTGCTCGACTCCCCCATGATGCTCCCCAATTCTCAGGCATTGCCATCTCCCACTACCGGAACTTTTCCATTGCTTCCTCCTAGTGATGACAGTTCAATGCTGAAATCTGCAACTAATGAAGATGTTCATAGGGGGAGTGGTTTTGATTCATCCTTCATGTTGAAGAGTGAAGGAGATCCTAAGTATCTGCCAGCTGGTTATTCTGGTTTTGAAAATCAGGGATCTAATGTGGACTATCAAGCTCTTGTTTTGGAGCAACAGCCAATGGATTTTGATTTCCCGATGGAATTTCCTGAAGAAGTGAATGCAAAGCACTATGCTGTTGATCCATCTAATGATGTTAAGAATGTAAATAGTGTGGTAATGAATGAGAGCTGTGTTGACTTGCAAATGCCACATTCCAGCTTAGCTTCTTTTCCCAAAGAAGTTATTCATGGGGAGGATGTTGGGACCCATCAACTTTTGGAAGGAGACGACAAAGGGTACCCATCTCTGGGAATGGCAAGGAATTCAGAGGATGGATACAATTGGAGGAAGTATGGGCAGAAACAGGTTAAAGGTAGTGAATATCCAAGGAGCTACTATAAGTGCACACATCCAAATTGTCAGGTGAAGAAAAAGGTGGAACGATCCTTTGATGGTCAAATAACAGAAATCATTTACAAGGGAGCTCCTCATAACCATGCACAACCTCAGCCTAATCGACGAGCTGCAGCATCACTTGGATCAGCATTTTCATTTGACGAGATGTCAGAGATGGGTGAGGGCAATAGAACCTCTGTCAAAGTTGACGGTGACTCACTTTGGGCAAAGATTCAGTCAGGTGAGGATATTAAGAGTGGTTATGATGGGAGGCCTGATGGTCCGGAAAGGACTTCCTCAGCATCCGTTGTTACTGATCTTTCTGATCCATTATCGGCTACCCATGGAAAATCCCCGGGTATCTTTGAATCTGCAGACACTCCGGAGTTTTCATCGACACTTGCTAGTGTTGATGAAGATGACCAGGCCACCCTACGGAGCATATCACTTGGAGAGGATGCAGATAATGAAGAATCTGACTCAAAAAGAAGAAAGAAAGATAGCTGCATGATTGAAACAAGTATAGCTTCCAGGGCTGTCCGTGAACCAAGAGTGGTTGTCCAGATTGAGAGTGAAATCGACATACTTGATGATGGCTACCGTTGGCGGAAGTATGGACAAAAAGTTGTCAAAGGAAATCCAAACCCCAGGAGCTACTACAAATGTACAAGTGCGGGATGCTTAGTAAGGAAGCATGTGGAAAGGGCCTCCCATGATCTGAAATTCGTAATTACTACATACGAGGGAAAACACAACCATGAAGTGCCTGCAGCCAGAAACAGTAATCATATAAACTCAAGCAGTGCAAATGGAAATCCACCTGCTCAGTCAGCACTGGCGTTATCCAGAAATACCAACATTCCAAAACCTGAGACACAAGTACAAGATCTTGCGCCTCATTTTGATAGAAAACCAGAATTTCATGACGAATACCTGCGACCTACTTATCTTGGGAATTTCAACAATGACTTGAAATATGGAGCTTCCTCCATCTACCAAATGAAGTTCCCACCTCTTCAGAATGCAATGTCGTATGGCTCCTTTGGTCTAAATGCACCTCATCACAATGTGGCTCACCAGGCTGGTTCTGTTGCATCTGTTGTCCCAGACTTCTCCATGTCACTGCCCTTGAATCTTCCCCCATCTGGGAATCTTGGTCTCGCAGGTTTTGATTTCAATAATGGAAAGTCAGTTTATCCAGTGCAGCCTTATGTCTCAGGACAGCAGCTCCAGGAGAATGACTTAAGGTACCTGAAGCCAAAACAAGAGCAGAAGGATGATGGCCTCTATGATGCCTGCAGCCTGCCTATCATTGATCAGGGAAATGCATCGCTGTCACCATCCTCATCCTTACTCTATCACAGAATGATGGGAAGCTATTCATCATATTCATCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000578 GO:0003006 GO:0003674 GO:0003700 GO:0006355 GO:0007163 GO:0007275 GO:0007389 GO:0008150 GO:0009555 GO:0009790 GO:0009791 GO:0009793 GO:0009798 GO:0009880 GO:0009889 GO:0009942 GO:0009987 GO:0010154 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022414 GO:0030010 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0048229 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

WRKY

Protein Analysis

733

Amino Acids

80.38

Weight (kDa)

5.65

Isoelectric Point (pI)

54.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	246 - 302	1.7e-24	WRKY DNA -binding domain
WRKY	PF03106	465 - 522	1.1e-25	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0013690)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g00500
malus_domestica	MD04G1244700.v1.1 MD12G1260600.v1.1
prunus_persica	Prupe.6G361300_v2.0.a1
pyrus_communis	pycom04g21550 pycom12g23860
rosa_chinensis	RchiOBHm_Chr3g0447881
rosa_laevigata	RLG00000025964
rosa_multiflora	Rmu_sc0005969.1_g000001 Rmu_sc0020869.1_g000002 Rmu_sc0033808.1_g000003
rosa_roxburghii	Rroxscaffold_6G00426080
rosa_rugosa	Rorug02G0606800
rosa_samantha	Rh3AG006000 Rh3BG005700 Rh3CG005900 Rh3DG006200
rosa_wichuraiana	Rw3G000460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1633
Acc36I	ACCTGC	3 cut(s)	1633, 1746, 1958
Acc65I	GGTACC	2 cut(s)	705, 2052
AccB1I	GGYRCC	2 cut(s)	705, 2052
AccII	CGCG	1 cut(s)	130
AccIII	TCCGGA	2 cut(s)	1087, 1186
AciI	CCGC	5 cut(s)	60, 110, 219, 1411, 1478
AclWI	GGATC	7 cut(s)	125, 392, 451, 542, 846, 955, 1124
AcsI	RAATTY	5 cut(s)	509, 730, 1526, 1721, 1759
AcuI	CTGAAG	3 cut(s)	537, 1805, 2078
AdeI	CACNNNGTG	1 cut(s)	1873
AfaI	GTAC	4 cut(s)	707, 1471, 1686, 2054
AfiI	CCNNNNNNNGG	7 cut(s)	84, 278, 715, 716, 1239, 1949, 1950
AflII	CTTAAG	1 cut(s)	2047
AhdI	GACNNNNNGTC	1 cut(s)	970
AjnI	CCWGG	5 cut(s)	1227, 1334, 1451, 1881, 2034
Alw21I	GWGCWC	2 cut(s)	815, 895
Alw26I	GTCTC	5 cut(s)	183, 687, 1670, 1967, 2023
Alw44I	GTGCAC	1 cut(s)	811
AlwI	GGATC	7 cut(s)	125, 392, 451, 542, 846, 955, 1124
Ama87I	CYCGRG	1 cut(s)	1159
Aor13HI	TCCGGA	2 cut(s)	1087, 1186
AoxI	GGCC	4 cut(s)	1076, 1230, 1509, 2086
ApaLI	GTGCAC	1 cut(s)	811
ApeKI	GCWGC	8 cut(s)	89, 932, 935, 1308, 1574, 2008, 2029, 2103
ApoI	RAATTY	5 cut(s)	509, 730, 1526, 1721, 1759
ArsI	GACNNNNNNTTYG	2 cut(s)	1025, 1057
Asp700I	GAANNNNTTC	3 cut(s)	345, 651, 2182
Asp718I	GGTACC	2 cut(s)	705, 2052
AspLEI	GCGC	2 cut(s)	130, 1699
AspS9I	GGNCC	4 cut(s)	141, 672, 1085, 1509
AsuC2I	CCSGG	3 cut(s)	204, 1160, 1161
AsuHPI	GGTGA	6 cut(s)	844, 995, 1028, 1058, 1871, 2133
AvaI	CYCGRG	1 cut(s)	1159
AvaII	GGWCC	3 cut(s)	141, 672, 1085
BaeGI	GKGCMC	1 cut(s)	815
BanI	GGYRCC	2 cut(s)	705, 2052
BanII	GRGCYC	1 cut(s)	895
Bbv12I	GWGCWC	2 cut(s)	815, 895
BbvCI	CCTCAGC	2 cut(s)	915, 1102
BbvI	GCAGC	8 cut(s)	101, 919, 947, 1295, 1586, 2020, 2041, 2115
BceAI	ACGGC	1 cut(s)	141
BciT130I	CCWGG	5 cut(s)	1229, 1336, 1453, 1883, 2036
BciVI	GTATCC	1 cut(s)	737
BclI	TGATCA	1 cut(s)	2119
BcnI	CCSGG	3 cut(s)	204, 1160, 1161
BcoDI	GTCTC	5 cut(s)	183, 687, 1670, 1967, 2023
BfaI	CTAG	2 cut(s)	303, 1209
BfmI	CTRYAG	4 cut(s)	933, 1176, 1572, 2101
BfrI	CTTAAG	1 cut(s)	2047
BfuAI	ACCTGC	3 cut(s)	1633, 1746, 1958
BfuI	GTATCC	1 cut(s)	737
BglII	AGATCT	1 cut(s)	1690
BisI	GCNGC	8 cut(s)	90, 933, 936, 1309, 1575, 2009, 2030, 2104
BlpI	GCTNAGC	1 cut(s)	628
BlsI	GCNGC	8 cut(s)	91, 934, 937, 1310, 1576, 2010, 2031, 2105
Bme1390I	CCNGG	8 cut(s)	204, 1160, 1161, 1229, 1336, 1453, 1883, 2036
Bme18I	GGWCC	3 cut(s)	141, 672, 1085
BmeRI	GACNNNNNGTC	1 cut(s)	970
BmeT110I	CYCGRG	1 cut(s)	1159
BmgT120I	GGNCC	4 cut(s)	141, 672, 1085, 1509
BmiI	GGNNCC	5 cut(s)	673, 674, 707, 1843, 2054
BmrFI	CCNGG	8 cut(s)	204, 1160, 1161, 1229, 1336, 1453, 1883, 2036
BmsI	GCATC	9 cut(s)	216, 231, 947, 1115, 1252, 1472, 1907, 2086, 2141
BpmI	CTGGAG	1 cut(s)	2018
Bpu10I	CCTNAGC	2 cut(s)	915, 1102
Bpu1102I	GCTNAGC	1 cut(s)	628
BpuEI	CTTGAG	1 cut(s)	1585
BpuMI	CCSGG	3 cut(s)	204, 1160, 1161
BsaI	GGTCTC	2 cut(s)	183, 1967
BsaJI	CCNNGG	6 cut(s)	794, 1147, 1158, 1159, 1335, 1451
BsaWI	WCCGGW	4 cut(s)	166, 278, 1087, 1186
BsaXI	ACNNNNNCTCC	4 cut(s)	184, 214, 2016, 2046
Bsc4I	CCNNNNNNNGG	7 cut(s)	84, 278, 715, 716, 1239, 1949, 1950
Bse1I	ACTGG	2 cut(s)	1644, 2003
Bse3DI	GCAATG	3 cut(s)	260, 290, 1836
BseAI	TCCGGA	2 cut(s)	1087, 1186
BseBI	CCWGG	5 cut(s)	1229, 1336, 1453, 1883, 2036
BseDI	CCNNGG	6 cut(s)	794, 1147, 1158, 1159, 1335, 1451
BseLI	CCNNNNNNNGG	7 cut(s)	84, 278, 715, 716, 1239, 1949, 1950
BseMI	GCAATG	3 cut(s)	260, 290, 1836
BseMII	CTCAG	6 cut(s)	269, 929, 1116, 1643, 1665, 2034
BseNI	ACTGG	2 cut(s)	1644, 2003
BseRI	GAGGAG	2 cut(s)	97, 885
BseSI	GKGCMC	1 cut(s)	815
BseXI	GCAGC	8 cut(s)	101, 919, 947, 1295, 1586, 2020, 2041, 2115
BsgI	GTGCAG	1 cut(s)	2027
Bsh1236I	CGCG	1 cut(s)	130
BshFI	GGCC	4 cut(s)	1078, 1232, 1511, 2088
BshNI	GGYRCC	2 cut(s)	705, 2052
BsiHKAI	GWGCWC	2 cut(s)	815, 895
BsiHKCI	CYCGRG	1 cut(s)	1159
BsiSI	CCGG	6 cut(s)	167, 204, 279, 1088, 1160, 1187
BslFI	GGGAC	3 cut(s)	198, 685, 1892
BslI	CCNNNNNNNGG	7 cut(s)	84, 278, 715, 716, 1239, 1949, 1950
BsmAI	GTCTC	5 cut(s)	183, 687, 1670, 1967, 2023
BsmBI	CGTCTC	1 cut(s)	687
BsmFI	GGGAC	3 cut(s)	198, 685, 1892
BsmI	GAATGC	2 cut(s)	532, 1831
BsnI	GGCC	4 cut(s)	1078, 1232, 1511, 2088
Bso31I	GGTCTC	2 cut(s)	183, 1967
BsoBI	CYCGRG	1 cut(s)	1159
Bsp1286I	GDGCHC	2 cut(s)	815, 895
Bsp13I	TCCGGA	2 cut(s)	1087, 1186
Bsp1407I	TGTACA	1 cut(s)	1469
Bsp1720I	GCTNAGC	1 cut(s)	628
Bsp19I	CCATGG	1 cut(s)	1147
BspACI	CCGC	5 cut(s)	60, 110, 219, 1411, 1478
BspANI	GGCC	4 cut(s)	1078, 1232, 1511, 2088
BspCNI	CTCAG	6 cut(s)	268, 928, 1115, 1642, 1666, 2033
BspEI	TCCGGA	2 cut(s)	1087, 1186
BspFNI	CGCG	1 cut(s)	130
BspHI	TCATGA	1 cut(s)	1726
BspLI	GGNNCC	5 cut(s)	673, 674, 707, 1843, 2054
BspMAI	CTGCAG	4 cut(s)	937, 1180, 1576, 2105
BspMI	ACCTGC	3 cut(s)	1633, 1746, 1958
BspPI	GGATC	7 cut(s)	125, 392, 451, 542, 846, 955, 1124
BspT107I	GGYRCC	2 cut(s)	705, 2052
BspTI	CTTAAG	1 cut(s)	2047
BspTNI	GGTCTC	2 cut(s)	183, 1967
BsrDI	GCAATG	3 cut(s)	260, 290, 1836
BsrGI	TGTACA	1 cut(s)	1469
BsrI	ACTGG	2 cut(s)	1644, 2003
BssECI	CCNNGG	6 cut(s)	794, 1147, 1158, 1159, 1335, 1451
BssT1I	CCWWGG	2 cut(s)	794, 1147
Bst2UI	CCWGG	5 cut(s)	1229, 1336, 1453, 1883, 2036
Bst4CI	ACNGT	4 cut(s)	314, 1016, 1406, 1586
Bst6I	CTCTTC	2 cut(s)	380, 1824
BstAFI	CTTAAG	1 cut(s)	2047
BstAUI	TGTACA	1 cut(s)	1469
BstC8I	GCNNGC	5 cut(s)	9, 414, 1572, 2101, 2108
BstDEI	CTNAG	9 cut(s)	255, 402, 628, 915, 1102, 1486, 1629, 1674, 2020
BstDSI	CCRYGG	1 cut(s)	1147
BstFNI	CGCG	1 cut(s)	130
BstHHI	GCGC	2 cut(s)	130, 1699
BstMAI	GTCTC	5 cut(s)	183, 687, 1670, 1967, 2023
BstMWI	GCNNNNNNNGC	3 cut(s)	1337, 1634, 1883
BstNI	CCWGG	5 cut(s)	1229, 1336, 1453, 1883, 2036
BstNSI	RCATGY	1 cut(s)	1502
BstSCI	CCNGG	8 cut(s)	202, 1158, 1159, 1227, 1334, 1451, 1881, 2034
BstSFI	CTRYAG	4 cut(s)	933, 1176, 1572, 2101
BstSLI	GKGCMC	1 cut(s)	815
BstUI	CGCG	1 cut(s)	130
BstV1I	GCAGC	8 cut(s)	101, 919, 947, 1295, 1586, 2020, 2041, 2115
BstX2I	RGATCY	3 cut(s)	397, 443, 1690
BstYI	RGATCY	3 cut(s)	397, 443, 1690
BsuI	GTATCC	1 cut(s)	737
BsuRI	GGCC	4 cut(s)	1078, 1232, 1511, 2088
BtgI	CCRYGG	1 cut(s)	1147
BtgZI	GCGATG	1 cut(s)	2118
BtsI	GCAGTG	2 cut(s)	1612, 1925
BtsIMutI	CAGTG	4 cut(s)	1612, 1637, 1925, 2010
BveI	ACCTGC	3 cut(s)	1633, 1746, 1958
Cac8I	GCNNGC	5 cut(s)	9, 414, 1572, 2101, 2108
CciI	TCATGA	1 cut(s)	1726
CfoI	GCGC	2 cut(s)	130, 1699
Cfr13I	GGNCC	4 cut(s)	141, 672, 1085, 1509
Cfr9I	CCCGGG	1 cut(s)	1159
Csp6I	GTAC	4 cut(s)	706, 1470, 1685, 2053
CspCI	CAANNNNNGTGG	1 cut(s)	35
CviQI	GTAC	4 cut(s)	706, 1470, 1685, 2053
DdeI	CTNAG	9 cut(s)	255, 402, 628, 915, 1102, 1486, 1629, 1674, 2020
DraIII	CACNNNGTG	1 cut(s)	1873
DriI	GACNNNNNGTC	1 cut(s)	970
Eam1104I	CTCTTC	2 cut(s)	380, 1824
Eam1105I	GACNNNNNGTC	1 cut(s)	970
EarI	CTCTTC	2 cut(s)	380, 1824
EciI	GGCGGA	1 cut(s)	1426
Ecl136II	GAGCTC	1 cut(s)	893
Eco130I	CCWWGG	2 cut(s)	794, 1147
Eco147I	AGGCCT	1 cut(s)	1078
Eco24I	GRGCYC	1 cut(s)	895
Eco31I	GGTCTC	2 cut(s)	183, 1967
Eco47I	GGWCC	3 cut(s)	141, 672, 1085
Eco53kI	GAGCTC	1 cut(s)	893
Eco57I	CTGAAG	3 cut(s)	537, 1805, 2078
Eco88I	CYCGRG	1 cut(s)	1159
EcoICRI	GAGCTC	1 cut(s)	893
EcoO109I	RGGNCCY	2 cut(s)	672, 1509
EcoRI	GAATTC	1 cut(s)	730
EcoRII	CCWGG	5 cut(s)	1227, 1334, 1451, 1881, 2034
EcoT14I	CCWWGG	2 cut(s)	794, 1147
EcoT22I	ATGCAT	1 cut(s)	2134
EcoT38I	GRGCYC	1 cut(s)	895
ErhI	CCWWGG	2 cut(s)	794, 1147
Esp3I	CGTCTC	1 cut(s)	687
FalI	AAGNNNNNCTT	2 cut(s)	1315, 1347
FaqI	GGGAC	3 cut(s)	198, 685, 1892
FauI	CCCGC	2 cut(s)	53, 1471
FbaI	TGATCA	1 cut(s)	2119
Fnu4HI	GCNGC	8 cut(s)	90, 933, 936, 1309, 1575, 2009, 2030, 2104
FriOI	GRGCYC	1 cut(s)	895
Fsp4HI	GCNGC	8 cut(s)	90, 933, 936, 1309, 1575, 2009, 2030, 2104
FspBI	CTAG	2 cut(s)	303, 1209
GlaI	GCGC	2 cut(s)	129, 1698
GluI	GCNGC	8 cut(s)	90, 933, 936, 1309, 1575, 2009, 2030, 2104
GsuI	CTGGAG	1 cut(s)	2018
HaeIII	GGCC	4 cut(s)	1078, 1232, 1511, 2088
HapII	CCGG	6 cut(s)	167, 204, 279, 1088, 1160, 1187
HhaI	GCGC	2 cut(s)	130, 1699
Hin6I	GCGC	2 cut(s)	128, 1697
HinP1I	GCGC	2 cut(s)	128, 1697
HincII	GTYRAC	2 cut(s)	604, 1012
HindII	GTYRAC	2 cut(s)	604, 1012
HpaII	CCGG	6 cut(s)	167, 204, 279, 1088, 1160, 1187
HphI	GGTGA	6 cut(s)	844, 995, 1028, 1058, 1871, 2133
Hpy166II	GTNNAC	5 cut(s)	454, 604, 813, 1012, 1349
Hpy188I	TCNGA	6 cut(s)	736, 976, 1129, 1282, 1524, 1824
Hpy188III	TCNNGA	9 cut(s)	136, 502, 515, 1088, 1187, 1366, 1650, 1727, 2022
Hpy8I	GTNNAC	5 cut(s)	454, 604, 813, 1012, 1349
Hpy99I	CGWCG	1 cut(s)	930
HpyAV	CCTTC	5 cut(s)	171, 385, 386, 683, 2071
HpyCH4III	ACNGT	4 cut(s)	314, 1016, 1406, 1586
HpyF10VI	GCNNNNNNNGC	3 cut(s)	1337, 1634, 1883
HpyF3I	CTNAG	9 cut(s)	255, 402, 628, 915, 1102, 1486, 1629, 1674, 2020
HspAI	GCGC	2 cut(s)	128, 1697
KflI	GGGWCCC	1 cut(s)	672
Kpn2I	TCCGGA	2 cut(s)	1087, 1186
KpnI	GGTACC	2 cut(s)	709, 2056
Ksp22I	TGATCA	1 cut(s)	2119
Lsp1109I	GCAGC	8 cut(s)	101, 919, 947, 1295, 1586, 2020, 2041, 2115
LweI	GCATC	9 cut(s)	216, 231, 947, 1115, 1252, 1472, 1907, 2086, 2141
MaeI	CTAG	2 cut(s)	303, 1209
MaeIII	GTNAC	4 cut(s)	1016, 1114, 1923, 2139
MboII	GAAGA	9 cut(s)	353, 397, 530, 847, 1232, 1286, 1305, 1811, 1931
MfeI	CAATTG	1 cut(s)	748
MflI	RGATCY	3 cut(s)	397, 443, 1690
MhlI	GDGCHC	2 cut(s)	815, 895
MluCI	AATT	9 cut(s)	250, 509, 730, 748, 823, 1526, 1533, 1721, 1759
MlyI	GAGTC	3 cut(s)	224, 1013, 1277
Mph1103I	ATGCAT	1 cut(s)	2134
MroI	TCCGGA	2 cut(s)	1087, 1186
MroXI	GAANNNNTTC	3 cut(s)	345, 651, 2182
MseI	TTAA	4 cut(s)	564, 777, 1056, 2048
MslI	CAYNNNNRTG	3 cut(s)	903, 1560, 1869
MspA1I	CMGCKG	1 cut(s)	416
MspCI	CTTAAG	1 cut(s)	2047
MspI	CCGG	6 cut(s)	167, 204, 279, 1088, 1160, 1187
MspR9I	CCNGG	8 cut(s)	204, 1160, 1161, 1229, 1336, 1453, 1883, 2036
MunI	CAATTG	1 cut(s)	748
Mva1269I	GAATGC	2 cut(s)	532, 1831
MvaI	CCWGG	5 cut(s)	1229, 1336, 1453, 1883, 2036
MvnI	CGCG	1 cut(s)	130
MwoI	GCNNNNNNNGC	3 cut(s)	1337, 1634, 1883
NciI	CCSGG	3 cut(s)	204, 1160, 1161
NcoI	CCATGG	1 cut(s)	1147
NlaIV	GGNNCC	5 cut(s)	673, 674, 707, 1843, 2054
NmeAIII	GCCGAG	1 cut(s)	103
NmuCI	GTSAC	3 cut(s)	1016, 1923, 2139
NsiI	ATGCAT	1 cut(s)	2134
NspI	RCATGY	1 cut(s)	1502
PagI	TCATGA	1 cut(s)	1726
PaqCI	CACCTGC	1 cut(s)	1633
PceI	AGGCCT	1 cut(s)	1078
PcsI	WCGNNNNNNNCGW	1 cut(s)	21
PctI	GAATGC	2 cut(s)	532, 1831
PdmI	GAANNNNTTC	3 cut(s)	345, 651, 2182
PfeI	GAWTC	7 cut(s)	102, 368, 1036, 1172, 1277, 1936, 1954
PfoI	TCCNGGA	1 cut(s)	2034
PkrI	GCNGC	8 cut(s)	91, 934, 937, 1310, 1576, 2010, 2031, 2105
PleI	GAGTC	3 cut(s)	224, 1013, 1277
PpsI	GAGTC	3 cut(s)	224, 1013, 1277
PpuMI	RGGWCCY	1 cut(s)	672
Psp124BI	GAGCTC	1 cut(s)	895
Psp5II	RGGWCCY	1 cut(s)	672
Psp6I	CCWGG	5 cut(s)	1227, 1334, 1451, 1881, 2034
PspGI	CCWGG	5 cut(s)	1227, 1334, 1451, 1881, 2034
PspN4I	GGNNCC	5 cut(s)	673, 674, 707, 1843, 2054
PspPI	GGNCC	4 cut(s)	141, 672, 1085, 1509
PspPPI	RGGWCCY	1 cut(s)	672
PstI	CTGCAG	4 cut(s)	937, 1180, 1576, 2105
PsuI	RGATCY	3 cut(s)	397, 443, 1690
PvuII	CAGCTG	1 cut(s)	416
RsaI	GTAC	4 cut(s)	707, 1471, 1686, 2054
RsaNI	GTAC	4 cut(s)	706, 1470, 1685, 2053
RseI	CAYNNNNRTG	3 cut(s)	903, 1560, 1869
SacI	GAGCTC	1 cut(s)	895
SaqAI	TTAA	4 cut(s)	564, 777, 1056, 2048
SatI	GCNGC	8 cut(s)	90, 933, 936, 1309, 1575, 2009, 2030, 2104
Sau96I	GGNCC	4 cut(s)	141, 672, 1085, 1509
SchI	GAGTC	3 cut(s)	224, 1013, 1277
ScrFI	CCNGG	8 cut(s)	204, 1160, 1161, 1229, 1336, 1453, 1883, 2036
SduI	GDGCHC	2 cut(s)	815, 895
SfaNI	GCATC	9 cut(s)	216, 231, 947, 1115, 1252, 1472, 1907, 2086, 2141
SfcI	CTRYAG	4 cut(s)	933, 1176, 1572, 2101
SinI	GGWCC	3 cut(s)	141, 672, 1085
SmaI	CCCGGG	1 cut(s)	1161
SmiMI	CAYNNNNRTG	3 cut(s)	903, 1560, 1869
SmlI	CTYRAG	2 cut(s)	1600, 2047
SmoI	CTYRAG	2 cut(s)	1600, 2047
Sse9I	AATT	9 cut(s)	250, 509, 730, 748, 823, 1526, 1533, 1721, 1759
SseBI	AGGCCT	1 cut(s)	1078
SsiI	CCGC	5 cut(s)	60, 110, 219, 1411, 1478
SspMI	CTAG	2 cut(s)	303, 1209
SstI	GAGCTC	1 cut(s)	895
StuI	AGGCCT	1 cut(s)	1078
StyD4I	CCNGG	8 cut(s)	202, 1158, 1159, 1227, 1334, 1451, 1881, 2034
StyI	CCWWGG	2 cut(s)	794, 1147
TaaI	ACNGT	4 cut(s)	314, 1016, 1406, 1586
TaqI	TCGA	4 cut(s)	228, 925, 1199, 1383
TasI	AATT	9 cut(s)	250, 509, 730, 748, 823, 1526, 1533, 1721, 1759
TatI	WGTACW	2 cut(s)	1469, 1684
TfiI	GAWTC	7 cut(s)	102, 368, 1036, 1172, 1277, 1936, 1954
Tru1I	TTAA	4 cut(s)	564, 777, 1056, 2048
Tru9I	TTAA	4 cut(s)	564, 777, 1056, 2048
TscAI	CASTG	4 cut(s)	1612, 1644, 1932, 2010
TseFI	GTSAC	3 cut(s)	1016, 1923, 2139
TseI	GCWGC	8 cut(s)	89, 932, 935, 1308, 1574, 2008, 2029, 2103
Tsp45I	GTSAC	3 cut(s)	1016, 1923, 2139
TspGWI	ACGGA	3 cut(s)	1099, 1255, 1334
TspMI	CCCGGG	1 cut(s)	1159
TspRI	CASTG	4 cut(s)	1612, 1644, 1932, 2010
Vha464I	CTTAAG	1 cut(s)	2047
VneI	GTGCAC	1 cut(s)	811
VpaK11BI	GGWCC	3 cut(s)	141, 672, 1085
XapI	RAATTY	5 cut(s)	509, 730, 1526, 1721, 1759
XceI	RCATGY	1 cut(s)	1502
XmaI	CCCGGG	1 cut(s)	1159
XmnI	GAANNNNTTC	3 cut(s)	345, 651, 2182
XspI	CTAG	2 cut(s)	303, 1209
Zsp2I	ATGCAT	1 cut(s)	2134

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.