Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0013690

Physical Location & Seq

Genomic Coordinates Reverse (-)

32647306 .. 32651367

Gene Structure 4062 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1260600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2157 bp

ATGAGACGTTTAAGAAAAATGGAGCAAGAAGACGACGCGGTTGCGGAGAGCAAGGCCAAAGGAAGAGGAGGCGGTGGTGGTGGTGGAGCAGGGAGCAGCATTAGCATAGCAGAGAGGAGGGCAGCCAAGTGTGGATTCAATGCGGAGAGGATCAATACGGCGCGTTTTAGGACAACCAGTCCTCTGCATTCTCCGCCGGCGAGGTCCCCGTGCCTTACTATTCCGGCTGGTATCAGCCCCACGGCTCTGCTCGACTCCCCCATGATGCTCCCCAATTCTCAGGCGCTTCCATCTCCCACCACCGGAACTTTTCCATTGCATCCTCCCAATGATAACAGCTCGCTGCTGAAATCAGGAACGCATGAAGATGATGGTTACAGGCCGGCCTATTCTAGTTTCCAAAATCCGGGGTCTACTGTTGACTGCCAAGCTCTTGTTTTGGAGCAACCACCAATGGAGTTTCAATTCCCGATGGAGTTTCCAGAAGAAGCTAATGCAACGAACTATGCTGTGGATCTGTCCTCTAATATTAGAAACTTAAACAGTGTGATTATGATTGCCAACGGCGCAGACTTGCAAATGTCTCATTCCAGTGCGGCTAGTGATCAAAACTTTCTTCCCAAAGAATCGATTCATGTGGAGGATCTTGGGTCCCATCACTTTTTGGAAGGAGATCAAAAAGGGTCATACCCATCTGCAGGAATGGTAAGGACTTCAGAAGATGGATACAATTGGAGGAAGTATGGGCAGAAACAGGTAAAAGGTAGTGAATATCCAAGGAGTTATTATAAATGCACGCACCCAAATTGTCAGGTCAAGAAAAAGGTGGAACGATCCTATGATGGTCAAATAACAGAGATCATCTACAAGGGAGCTCCTCATAATCATGCAAAACCGCAGCCTAACCGTCGAACTGGGGCATCGCTTGGACCATCATTTTCATATGATGAGATGTCAGAGATGGGTGAAGGTAGTAGGGCCGCTGTCAAAGGTGAAGGTGGGCCAGTTTGGACAAATAGTCAGTCCTGTAAGGATATGAAAGTTGGCTATGACGGGAGGACTGATGGTCTGGAAAGGACATCCTCAGCATCTGTTGTTACCGAGCTTTCTGATCCATTTTCAACTACTCAAGGGAAGTCCATGAATATCTTTGAATCAGCTGAAACTCCAGAGCTTTCATCCACACTTGCTAGTCATGATGATGAAGATCGGGCCACCCAAGGAATCATATCACTTGGAGATGATGCAGATGATGAAGAATCTGATTCAAAAAGAAGGAAGAAGGAGAGCTGCATGATTGAAACAAATTTAGCATCCAGGGCTGTCCGTGAACCAAGAGTAGTTGTCCAAATTGAGAGCGAAATCGACATACTTGATGATGGCTATCGCTGGCGGAAGTATGGGCAAAAAGTGGTCAAAGGAAATCCAAACCCTAGGAGCTATTACAAATGCACAAGTGCAGGATGTTTGGTTAGGAAGCATGTGGAAAGGGCGTCCCATGATCTGAAATTCGTAATTACAACATACGAGGGAAAACACAACCACGAAGTGCCTGCAGCGAGAAACAGCAATCACATGAATTCAATTAGCAGTAATGGACTTCCATCTACTGCCAATGCTCAGCCAGCTTTGGCATTACCCAGAAATACCAATGTTCCGAAGCCTGAGACACAAGTGCAAGATCTTGCACCTCATTTTGATAGAAAACCGGAATTTCATGACGCATACATGAGACCTAATTTTCTTGGGAATTTCAATAATGACATGAAATTTGGAGCTTCCTCCCTCTACCAAATGAAATATCCTCCTCTGCACAATACCATGCCGTATGCCTCATTCGGACACAATGTTGCCCATCAGGCTGGTTCTATTGTGCCGGACTTCCCAATTTCACTGCCTATGAACCTTCAGCCATCCGGGAACCTCCCTCTTGCCGGTTATGACATCAACAATGGAAAAACAGTTTGTCCCGTACAACCTTATTTTTCAGGACAGCATCTGCAGGAGAATGATGTAAGATTCCTAAAGCCTAAACAAGAGCATAAGGATGATAACCTTTATGATGCCCGCTTACCAATAATGGATCAGTCAAGTGCATCATTGTCGTCGTCATCATCGCTGTTCTATCACAGGGTCATGGGAGGTTATCCATCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000578 GO:0003006 GO:0003674 GO:0003700 GO:0006355 GO:0007163 GO:0007275 GO:0007389 GO:0008150 GO:0009555 GO:0009790 GO:0009791 GO:0009793 GO:0009798 GO:0009880 GO:0009889 GO:0009942 GO:0009987 GO:0010154 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022414 GO:0030010 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0048229 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

WRKY

Protein Analysis

719

Amino Acids

78.96

Weight (kDa)

6.4

Isoelectric Point (pI)

53.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	240 - 296	3.3e-24	WRKY DNA -binding domain
WRKY	PF03106	459 - 516	1e-25	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013690)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g00500
malus_domestica	MD04G1244700.v1.1 MD12G1260600.v1.1
prunus_persica	Prupe.6G361300_v2.0.a1
pyrus_communis	pycom04g21550 pycom12g23860
rosa_chinensis	RchiOBHm_Chr3g0447881
rosa_laevigata	RLG00000025964
rosa_multiflora	Rmu_sc0005969.1_g000001 Rmu_sc0020869.1_g000002 Rmu_sc0033808.1_g000003
rosa_roxburghii	Rroxscaffold_6G00426080
rosa_rugosa	Rorug02G0606800
rosa_samantha	Rh3AG006000 Rh3BG005700 Rh3CG005900 Rh3DG006200
rosa_wichuraiana	Rw3G000460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	789
AccI	GTMKAC	1 cut(s)	413
AccII	CGCG	2 cut(s)	38, 163
AclWI	GGATC	6 cut(s)	158, 522, 651, 828, 1106, 2091
AcsI	RAATTY	6 cut(s)	1306, 1508, 1579, 1712, 1750, 1769
AcuI	CTGAAG	2 cut(s)	699, 1892
AcyI	GRCGYC	1 cut(s)	1493
AdeI	CACNNNGTG	1 cut(s)	1549
AfaI	GTAC	1 cut(s)	1974
AfiI	CCNNNNNNNGG	4 cut(s)	302, 406, 698, 1934
AgsI	TTSAA	8 cut(s)	139, 464, 1122, 1154, 1269, 1301, 1584, 1756
AhdI	GACNNNNNGTC	3 cut(s)	177, 1017, 1065
AjnI	CCWGG	1 cut(s)	1316
AjuI	GAANNNNNNNTTGG	2 cut(s)	119, 151
Alw21I	GWGCWC	1 cut(s)	877
Alw26I	GTCTC	3 cut(s)	588, 1661, 1726
AlwI	GGATC	6 cut(s)	158, 522, 651, 828, 1106, 2091
AlwNI	CAGNNNCTG	2 cut(s)	1091, 1999
AoxI	GGCC	6 cut(s)	54, 380, 384, 978, 1001, 1212
ApeKI	GCWGC	6 cut(s)	96, 122, 343, 898, 1290, 1556
ApoI	RAATTY	6 cut(s)	1306, 1508, 1579, 1712, 1750, 1769
ArsI	GACNNNNNNTTYG	4 cut(s)	1007, 1039, 1754, 1786
Asp700I	GAANNNNTTC	1 cut(s)	630
AspA2I	CCTAGG	1 cut(s)	1433
AspLEI	GCGC	3 cut(s)	163, 286, 569
AspS9I	GGNCC	6 cut(s)	204, 651, 929, 978, 1001, 1212
AsuC2I	CCSGG	2 cut(s)	408, 1918
AsuHPI	GGTGA	2 cut(s)	977, 1004
AvaII	GGWCC	3 cut(s)	204, 651, 929
AvrII	CCTAGG	1 cut(s)	1433
BanII	GRGCYC	1 cut(s)	877
BbsI	GAAGAC	1 cut(s)	36
Bbv12I	GWGCWC	1 cut(s)	877
BbvCI	CCTCAGC	1 cut(s)	1084
BbvI	GCAGC	6 cut(s)	108, 134, 330, 910, 1277, 1568
BceAI	ACGGC	4 cut(s)	174, 258, 580, 1810
BcgI	CGANNNNNNTGC	6 cut(s)	23, 57, 1535, 1569, 2085, 2119
BciT130I	CCWGG	1 cut(s)	1318
BciVI	GTATCC	1 cut(s)	719
BclI	TGATCA	1 cut(s)	604
BcnI	CCSGG	2 cut(s)	408, 1918
BcoDI	GTCTC	3 cut(s)	588, 1661, 1726
BfaI	CTAG	4 cut(s)	393, 600, 1191, 1434
BfmI	CTRYAG	3 cut(s)	696, 1554, 2000
BfoI	RGCGCY	1 cut(s)	287
BfuI	GTATCC	1 cut(s)	719
BglI	GCCNNNNNGGC	1 cut(s)	1859
BglII	AGATCT	1 cut(s)	1681
BisI	GCNGC	8 cut(s)	97, 123, 344, 597, 899, 981, 1291, 1557
BlnI	CCTAGG	1 cut(s)	1433
BlpI	GCTNAGC	1 cut(s)	1620
BlsI	GCNGC	8 cut(s)	98, 124, 345, 598, 900, 982, 1292, 1558
Bme1390I	CCNGG	3 cut(s)	408, 1318, 1918
Bme18I	GGWCC	3 cut(s)	204, 651, 929
BmeRI	GACNNNNNGTC	3 cut(s)	177, 1017, 1065
BmgT120I	GGNCC	6 cut(s)	204, 651, 929, 978, 1001, 1212
BmiI	GGNNCC	4 cut(s)	206, 652, 653, 1922
BmrFI	CCNGG	3 cut(s)	408, 1318, 1918
BmrI	ACTGGG	1 cut(s)	924
BmsI	GCATC	9 cut(s)	255, 328, 929, 1097, 1234, 1322, 2005, 2053, 2105
BmuI	ACTGGG	1 cut(s)	924
BpiI	GAAGAC	1 cut(s)	36
BpmI	CTGGAG	1 cut(s)	1152
Bpu10I	CCTNAGC	1 cut(s)	1084
Bpu1102I	GCTNAGC	1 cut(s)	1620
BpuEI	CTTGAG	1 cut(s)	1113
BpuMI	CCSGG	2 cut(s)	408, 1918
Bsa29I	ATCGAT	1 cut(s)	629
BsaBI	GATNNNNATC	2 cut(s)	1206, 1383
BsaHI	GRCGYC	1 cut(s)	1493
BsaI	GGTCTC	1 cut(s)	1726
BsaJI	CCNNGG	6 cut(s)	240, 407, 776, 1219, 1317, 1433
BsaWI	WCCGGW	2 cut(s)	302, 1708
Bsc4I	CCNNNNNNNGG	4 cut(s)	302, 406, 698, 1934
Bse118I	RCCGGY	3 cut(s)	196, 382, 1934
Bse1I	ACTGG	4 cut(s)	177, 591, 919, 1004
Bse3DI	GCAATG	1 cut(s)	314
Bse8I	GATNNNNATC	2 cut(s)	1206, 1383
BseBI	CCWGG	1 cut(s)	1318
BseCI	ATCGAT	1 cut(s)	629
BseDI	CCNNGG	6 cut(s)	240, 407, 776, 1219, 1317, 1433
BseGI	GGATG	7 cut(s)	319, 1079, 1178, 1313, 1469, 1913, 2053
BseJI	GATNNNNATC	2 cut(s)	1206, 1383
BseLI	CCNNNNNNNGG	4 cut(s)	302, 406, 698, 1934
BseMI	GCAATG	1 cut(s)	314
BseMII	CTCAG	4 cut(s)	293, 1098, 1634, 1656
BseNI	ACTGG	4 cut(s)	177, 591, 919, 1004
BseRI	GAGGAG	4 cut(s)	81, 130, 867, 1797
BseXI	GCAGC	6 cut(s)	108, 134, 330, 910, 1277, 1568
BsgI	GTGCAG	2 cut(s)	1479, 1796
Bsh1236I	CGCG	2 cut(s)	38, 163
BshFI	GGCC	6 cut(s)	56, 382, 386, 980, 1003, 1214
BshVI	ATCGAT	1 cut(s)	629
BsiHKAI	GWGCWC	1 cut(s)	877
BsiSI	CCGG	9 cut(s)	197, 224, 303, 383, 407, 1709, 1877, 1917, 1935
BslFI	GGGAC	4 cut(s)	190, 637, 1480, 1953
BslI	CCNNNNNNNGG	4 cut(s)	302, 406, 698, 1934
BsmAI	GTCTC	3 cut(s)	588, 1661, 1726
BsmFI	GGGAC	4 cut(s)	190, 637, 1480, 1953
BsmI	GAATGC	1 cut(s)	187
BsnI	GGCC	6 cut(s)	56, 382, 386, 980, 1003, 1214
Bso31I	GGTCTC	1 cut(s)	1726
Bsp1286I	GDGCHC	1 cut(s)	877
Bsp1720I	GCTNAGC	1 cut(s)	1620
BspANI	GGCC	6 cut(s)	56, 382, 386, 980, 1003, 1214
BspCNI	CTCAG	4 cut(s)	292, 1097, 1633, 1657
BspDI	ATCGAT	1 cut(s)	629
BspFNI	CGCG	2 cut(s)	38, 163
BspHI	TCATGA	2 cut(s)	1195, 1717
BspLI	GGNNCC	4 cut(s)	206, 652, 653, 1922
BspMAI	CTGCAG	3 cut(s)	700, 1558, 2004
BspPI	GGATC	6 cut(s)	158, 522, 651, 828, 1106, 2091
BspTNI	GGTCTC	1 cut(s)	1726
BsrDI	GCAATG	1 cut(s)	314
BsrFI	RCCGGY	3 cut(s)	196, 382, 1934
BsrI	ACTGG	4 cut(s)	177, 591, 919, 1004
BssAI	RCCGGY	3 cut(s)	196, 382, 1934
BssECI	CCNNGG	6 cut(s)	240, 407, 776, 1219, 1317, 1433
BssNI	GRCGYC	1 cut(s)	1493
BssT1I	CCWWGG	3 cut(s)	776, 1219, 1433
Bst2UI	CCWGG	1 cut(s)	1318
Bst4CI	ACNGT	4 cut(s)	418, 545, 908, 1963
Bst6I	CTCTTC	1 cut(s)	58
BstACI	GRCGYC	1 cut(s)	1493
BstC8I	GCNNGC	8 cut(s)	198, 341, 384, 797, 1391, 1554, 1626, 2068
BstDEI	CTNAG	4 cut(s)	279, 1084, 1620, 1665
BstDSI	CCRYGG	1 cut(s)	240
BstF5I	GGATG	7 cut(s)	319, 1079, 1178, 1313, 1469, 1913, 2053
BstFNI	CGCG	2 cut(s)	38, 163
BstH2I	RGCGCY	1 cut(s)	287
BstHHI	GCGC	3 cut(s)	163, 286, 569
BstMAI	GTCTC	3 cut(s)	588, 1661, 1726
BstMWI	GCNNNNNNNGC	6 cut(s)	102, 193, 566, 1319, 1625, 1859
BstNI	CCWGG	1 cut(s)	1318
BstNSI	RCATGY	1 cut(s)	1484
BstSCI	CCNGG	3 cut(s)	406, 1316, 1916
BstSFI	CTRYAG	3 cut(s)	696, 1554, 2000
BstUI	CGCG	2 cut(s)	38, 163
BstV1I	GCAGC	6 cut(s)	108, 134, 330, 910, 1277, 1568
BstV2I	GAAGAC	1 cut(s)	36
BstX2I	RGATCY	3 cut(s)	514, 643, 1681
BstXI	CCANNNNNNTGG	1 cut(s)	1862
BstYI	RGATCY	3 cut(s)	514, 643, 1681
Bsu15I	ATCGAT	1 cut(s)	629
BsuI	GTATCC	1 cut(s)	719
BsuRI	GGCC	6 cut(s)	56, 382, 386, 980, 1003, 1214
BsuTUI	ATCGAT	1 cut(s)	629
BtgI	CCRYGG	1 cut(s)	240
BtgZI	GCGATG	2 cut(s)	906, 2100
BtsCI	GGATG	7 cut(s)	319, 1079, 1178, 1313, 1469, 1913, 2053
BtsI	GCAGTG	1 cut(s)	1892
BtsIMutI	CAGTG	3 cut(s)	550, 598, 1892
Cac8I	GCNNGC	8 cut(s)	198, 341, 384, 797, 1391, 1554, 1626, 2068
CaiI	CAGNNNCTG	2 cut(s)	1091, 1999
CciI	TCATGA	2 cut(s)	1195, 1717
CfoI	GCGC	3 cut(s)	163, 286, 569
Cfr10I	RCCGGY	3 cut(s)	196, 382, 1934
Cfr13I	GGNCC	6 cut(s)	204, 651, 929, 978, 1001, 1212
ClaI	ATCGAT	1 cut(s)	629
CseI	GACGC	3 cut(s)	44, 1482, 1730
Csp6I	GTAC	1 cut(s)	1973
CviQI	GTAC	1 cut(s)	1973
DdeI	CTNAG	4 cut(s)	279, 1084, 1620, 1665
DraIII	CACNNNGTG	1 cut(s)	1549
DriI	GACNNNNNGTC	3 cut(s)	177, 1017, 1065
Eam1104I	CTCTTC	1 cut(s)	58
Eam1105I	GACNNNNNGTC	3 cut(s)	177, 1017, 1065
EarI	CTCTTC	1 cut(s)	58
EciI	GGCGGA	2 cut(s)	183, 1408
Ecl136II	GAGCTC	1 cut(s)	875
Eco130I	CCWWGG	3 cut(s)	776, 1219, 1433
Eco24I	GRGCYC	1 cut(s)	877
Eco31I	GGTCTC	1 cut(s)	1726
Eco47I	GGWCC	3 cut(s)	204, 651, 929
Eco53kI	GAGCTC	1 cut(s)	875
Eco57I	CTGAAG	2 cut(s)	699, 1892
EcoICRI	GAGCTC	1 cut(s)	875
EcoO109I	RGGNCCY	2 cut(s)	204, 651
EcoRI	GAATTC	1 cut(s)	1579
EcoRII	CCWGG	1 cut(s)	1316
EcoT14I	CCWWGG	3 cut(s)	776, 1219, 1433
EcoT38I	GRGCYC	1 cut(s)	877
ErhI	CCWWGG	3 cut(s)	776, 1219, 1433
FaqI	GGGAC	4 cut(s)	190, 637, 1480, 1953
FauI	CCCGC	1 cut(s)	2075
FauNDI	CATATG	1 cut(s)	943
FbaI	TGATCA	1 cut(s)	604
FblI	GTMKAC	1 cut(s)	413
Fnu4HI	GCNGC	8 cut(s)	97, 123, 344, 597, 899, 981, 1291, 1557
FokI	GGATG	7 cut(s)	306, 1066, 1165, 1300, 1476, 1900, 2060
FriOI	GRGCYC	1 cut(s)	877
FseI	GGCCGGCC	1 cut(s)	386
Fsp4HI	GCNGC	8 cut(s)	97, 123, 344, 597, 899, 981, 1291, 1557
FspBI	CTAG	4 cut(s)	393, 600, 1191, 1434
GlaI	GCGC	3 cut(s)	162, 285, 568
GluI	GCNGC	8 cut(s)	97, 123, 344, 597, 899, 981, 1291, 1557
GsuI	CTGGAG	1 cut(s)	1152
HaeII	RGCGCY	1 cut(s)	287
HaeIII	GGCC	6 cut(s)	56, 382, 386, 980, 1003, 1214
HapII	CCGG	9 cut(s)	197, 224, 303, 383, 407, 1709, 1877, 1917, 1935
HgaI	GACGC	3 cut(s)	44, 1482, 1730
HhaI	GCGC	3 cut(s)	163, 286, 569
Hin1I	GRCGYC	1 cut(s)	1493
Hin6I	GCGC	3 cut(s)	161, 284, 567
HinP1I	GCGC	3 cut(s)	161, 284, 567
HincII	GTYRAC	1 cut(s)	421
HindII	GTYRAC	1 cut(s)	421
HinfI	GANTC	9 cut(s)	135, 254, 626, 631, 1154, 1224, 1259, 1265, 2019
HpaII	CCGG	9 cut(s)	197, 224, 303, 383, 407, 1709, 1877, 1917, 1935
HphI	GGTGA	2 cut(s)	977, 1004
Hpy166II	GTNNAC	3 cut(s)	414, 421, 1331
Hpy188I	TCNGA	7 cut(s)	718, 958, 1111, 1264, 1506, 1659, 1841
Hpy188III	TCNNGA	9 cut(s)	354, 469, 482, 817, 1070, 1169, 1196, 1718, 1989
Hpy8I	GTNNAC	3 cut(s)	414, 421, 1331
Hpy99I	CGWCG	3 cut(s)	38, 912, 2110
HpyAV	CCTTC	6 cut(s)	662, 962, 989, 1269, 1276, 1916
HpyCH4III	ACNGT	4 cut(s)	418, 545, 908, 1963
HpyCH4IV	ACGT	1 cut(s)	7
HpyF10VI	GCNNNNNNNGC	6 cut(s)	102, 193, 566, 1319, 1625, 1859
HpyF3I	CTNAG	4 cut(s)	279, 1084, 1620, 1665
HpySE526I	ACGT	1 cut(s)	7
Hsp92I	GRCGYC	1 cut(s)	1493
HspAI	GCGC	3 cut(s)	161, 284, 567
KflI	GGGWCCC	1 cut(s)	651
KroI	GCCGGC	2 cut(s)	196, 382
KroNI	GCCGGC	2 cut(s)	198, 384
Ksp22I	TGATCA	1 cut(s)	604
LmnI	GCTCC	9 cut(s)	22, 86, 93, 273, 442, 872, 880, 1437, 1775
Lsp1109I	GCAGC	6 cut(s)	108, 134, 330, 910, 1277, 1568
LweI	GCATC	9 cut(s)	255, 328, 929, 1097, 1234, 1322, 2005, 2053, 2105
MaeI	CTAG	4 cut(s)	393, 600, 1191, 1434
MaeII	ACGT	1 cut(s)	7
MaeIII	GTNAC	2 cut(s)	374, 1096
MboII	GAAGA	9 cut(s)	41, 75, 377, 497, 608, 731, 1217, 1268, 1291
MfeI	CAATTG	1 cut(s)	730
MflI	RGATCY	3 cut(s)	514, 643, 1681
MhlI	GDGCHC	1 cut(s)	877
MlyI	GAGTC	1 cut(s)	248
MreI	CGCCGGCG	1 cut(s)	196
MroNI	GCCGGC	2 cut(s)	196, 382
MroXI	GAANNNNTTC	1 cut(s)	630
MseI	TTAA	2 cut(s)	11, 539
MslI	CAYNNNNRTG	5 cut(s)	366, 591, 885, 1200, 2046
MspA1I	CMGCKG	2 cut(s)	983, 1160
MspI	CCGG	9 cut(s)	197, 224, 303, 383, 407, 1709, 1877, 1917, 1935
MspR9I	CCNGG	3 cut(s)	408, 1318, 1918
MunI	CAATTG	1 cut(s)	730
Mva1269I	GAATGC	1 cut(s)	187
MvaI	CCWGG	1 cut(s)	1318
MvnI	CGCG	2 cut(s)	38, 163
MwoI	GCNNNNNNNGC	6 cut(s)	102, 193, 566, 1319, 1625, 1859
NaeI	GCCGGC	2 cut(s)	198, 384
NciI	CCSGG	2 cut(s)	408, 1918
NdeI	CATATG	1 cut(s)	943
NgoMIV	GCCGGC	2 cut(s)	196, 382
NlaIV	GGNNCC	4 cut(s)	206, 652, 653, 1922
NspI	RCATGY	1 cut(s)	1484
PagI	TCATGA	2 cut(s)	1195, 1717
PctI	GAATGC	1 cut(s)	187
PdiI	GCCGGC	2 cut(s)	198, 384
PdmI	GAANNNNTTC	1 cut(s)	630
PfeI	GAWTC	8 cut(s)	135, 626, 631, 1154, 1224, 1259, 1265, 2019
PfoI	TCCNGGA	1 cut(s)	1916
PkrI	GCNGC	8 cut(s)	98, 124, 345, 598, 900, 982, 1292, 1558
PleI	GAGTC	1 cut(s)	248
PpsI	GAGTC	1 cut(s)	248
PpuMI	RGGWCCY	2 cut(s)	204, 651
PsiI	TTATAA	1 cut(s)	789
Psp124BI	GAGCTC	1 cut(s)	877
Psp5II	RGGWCCY	2 cut(s)	204, 651
Psp6I	CCWGG	1 cut(s)	1316
PspGI	CCWGG	1 cut(s)	1316
PspN4I	GGNNCC	4 cut(s)	206, 652, 653, 1922
PspPI	GGNCC	6 cut(s)	204, 651, 929, 978, 1001, 1212
PspPPI	RGGWCCY	2 cut(s)	204, 651
PstI	CTGCAG	3 cut(s)	700, 1558, 2004
PstNI	CAGNNNCTG	2 cut(s)	1091, 1999
PsuI	RGATCY	3 cut(s)	514, 643, 1681
PvuII	CAGCTG	1 cut(s)	1160
RigI	GGCCGGCC	1 cut(s)	386
RsaI	GTAC	1 cut(s)	1974
RsaNI	GTAC	1 cut(s)	1973
RseI	CAYNNNNRTG	5 cut(s)	366, 591, 885, 1200, 2046
SacI	GAGCTC	1 cut(s)	877
SaqAI	TTAA	2 cut(s)	11, 539
SatI	GCNGC	8 cut(s)	97, 123, 344, 597, 899, 981, 1291, 1557
Sau96I	GGNCC	6 cut(s)	204, 651, 929, 978, 1001, 1212
SchI	GAGTC	1 cut(s)	248
ScrFI	CCNGG	3 cut(s)	408, 1318, 1918
SduI	GDGCHC	1 cut(s)	877
SfaNI	GCATC	9 cut(s)	255, 328, 929, 1097, 1234, 1322, 2005, 2053, 2105
SfcI	CTRYAG	3 cut(s)	696, 1554, 2000
SgrAI	CRCCGGYG	1 cut(s)	196
SinI	GGWCC	3 cut(s)	204, 651, 929
SmiMI	CAYNNNNRTG	5 cut(s)	366, 591, 885, 1200, 2046
SmlI	CTYRAG	1 cut(s)	1128
SmoI	CTYRAG	1 cut(s)	1128
SspI	AATATT	1 cut(s)	529
SspMI	CTAG	4 cut(s)	393, 600, 1191, 1434
SstI	GAGCTC	1 cut(s)	877
StyD4I	CCNGG	3 cut(s)	406, 1316, 1916
StyI	CCWWGG	3 cut(s)	776, 1219, 1433
TaaI	ACNGT	4 cut(s)	418, 545, 908, 1963
TaiI	ACGT	1 cut(s)	10
TaqI	TCGA	4 cut(s)	252, 629, 910, 1365
TauI	GCSGC	2 cut(s)	599, 983
TfiI	GAWTC	8 cut(s)	135, 626, 631, 1154, 1224, 1259, 1265, 2019
Tru1I	TTAA	2 cut(s)	11, 539
Tru9I	TTAA	2 cut(s)	11, 539
TscAI	CASTG	3 cut(s)	550, 598, 1899
TseI	GCWGC	6 cut(s)	96, 122, 343, 898, 1290, 1556
TspGWI	ACGGA	1 cut(s)	1316
TspRI	CASTG	3 cut(s)	550, 598, 1899
VpaK11BI	GGWCC	3 cut(s)	204, 651, 929
XapI	RAATTY	6 cut(s)	1306, 1508, 1579, 1712, 1750, 1769
XceI	RCATGY	1 cut(s)	1484
XmaJI	CCTAGG	1 cut(s)	1433
XmiI	GTMKAC	1 cut(s)	413
XmnI	GAANNNNTTC	1 cut(s)	630
XspI	CTAG	4 cut(s)	393, 600, 1191, 1434

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.