Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0008285

Physical Location & Seq

Genomic Coordinates Forward (+)

32135877 .. 32141110

Gene Structure 5234 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1246300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2058 bp

ATGTGTGGGATATTTGCTTATCTGAATTACAACGTCAACAGAGAGAGGCGTTACATTCTGCAGGTGCTATTCAATGGCCTGAGGCGTCTGGAATACCGGGGCTACGATTCCGCCGGGATTTCAATCGACCATTCTTATTCCTTCGATCCAAACACCCCCCAATCTTCCCCGCCTCCCCCCCTCGTTTTCCGCCAGGAGGGAAATATCGAGACCCTCGTCAAATCCGTCTACCAAGAGGTTGCTGAAACAGATTTAAATTTGGAAGAATCTTTCTCTCACCATGCTGGAATAGCGCACACCCGGTGGGCCACTCATGGAGAGCCAGCTCCAAGGAACAGCCATCCACAGAGCTCTGGTTCTGGAAATGAATTTTTGGTTGTTCACAATGGAGTCATCACTAATTATGAGGCCTTAAAGGAATCACTAGTTCGGCACGGATTCACCTTTGAATCTGAAACAGATACAGAAGTAATTCCAAAGCTTGCCAAATATGTTTATGATAAAGCTAAAGAAGGAGAAGGTGACCAGACTATCACATTTAGTCAAGTTGTGCTTGAGGTTATGAGGCATCTTGAAGGTGCATATGCCCTGATTTTTAAAAGCCAGCATTATCCAAATGAGTTGATTGCTTGCAAGCGTGGCAGTCCGTTGCTCCTTGGTGTCAAAGAATTTAACGAAAATGCTAGCGGTGGATCAGCATTTCACGATGATAATTTCCTTTCAAAAAGTGGACATGCTAAAGAACTCTTTTTGTCCAGTGATGCCAATGCTGTAGTTGAACACACTAAAAAGGTGTTGGTGATTGAGGATGGTGAAGTGGTACATCTGAAGGATGGAGGTGTTTCTATCCTGAAGTTTGATAAAGGACAGCATGGTGGACTTTCAAGAGTTGCATCAGTACAACGAGCGTTGTCCATTCTTGAGATGGAGGTTGAACAAATAAACAAAGGGAGTTACAAACATTATATGCAGAAAGAAATTCATGAACAGCCTGAATCTTTGACTACCACAATGAGGGGGAGGCTTTTACGTGGAGGTTCCTGCAAAGCCAAGACTGTTCTCCTTGGTGGACTGAAGGATCACCTTAAAACAATTAGGCGAAGCAGGAGAATTGTTTTCATCGGTTGTGGTACAAGCTACAATGCTGCTTTAGCTGCAAGACCCATCTTGGAAGAACTTTCTGGTATTCCTGTCACAATGGAAATTGCTAGTGATTTGTTGGATCGGCAAGGACCTATATATAGAGAAGATACAGCTGTCTTTGTCAGTCAATCTGGTGAAACAGCAGATACTCTGAGTGCTTTGGAATATGCGTTAGAAAACGGTGCATTATGCGTTGGCATAACTAATACTGTTGGTAGTGCTATAGCCAGGAATACACACTGCGGTGTTCATATAAATGCTGGCGCTGAGATTGGTGTGGCAAGCACCAAGGCATACACGAGTCAAATAGTGGTGATGGCTATGCTAGCACTAGCTATAGGAGGTGACACAATTTCCAATCAAGCACGAAGGGAGGCTATAATTGACGGTTTATTTGAGTTGCCAAACAAAGTCAGAGAGGTCCTGAAGCTTGACCAGATGATGAAGGATCTTGCACAGGAACTGATAGCTCATCAGTCACTTCTTGTGTTTGGGAGAGGATACAACTACGCAACATCTCTAGAGGGTGCTTTGAAGGTAAAGGAAGTGGCCCTTATGCACAGTGAAGGAATACTAGCGGGTGAAATGAAACATGGTCCTTTGGCTTTAGTTGATGAAACTCTCCCCACATTTGTGATTGCCACCCGTGATGCTTGCTTCAGTAAGCAGCAATCAGTTATTCAGCAGCTTCGTGCCCGTAAAGGTCGACTCATAGTAATGTGTTCGAAAGGAGATGCTGCATCAGTGTGCCCCGGGGGGTCTTGTCGAGTGATTGAAGTTCCTCAGCTTGAGGATTGTCTTCAACCCGTGGTTAATATTATTCCATTGCAGCTTCTGGCATACCATCTCACCGTTTTAAGAGGTTTTAACGTGGATCAGCCTCGGAATCTTGCGAAGAGCGTGACTACTCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004360 GO:0006002 GO:0006040 GO:0006047 GO:0006139 GO:0006464 GO:0006486 GO:0006487 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008483 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009225 GO:0009987 GO:0016740 GO:0016769 GO:0019538 GO:0019637 GO:0034641 GO:0034645 GO:0036211 GO:0043170 GO:0043412 GO:0043413 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0046483 GO:0055086 GO:0070085 GO:0070548 GO:0071704 GO:1901135 GO:1901137 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00250 ko00520 ko01100 ko01130 ko04931 map00250 map00520 map01100 map01130 map04931

Pfam Domains

Protein Families

GATase_6 SIS

Protein Analysis

686

Amino Acids

75.1

Weight (kDa)

6.33

Isoelectric Point (pI)

42.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GATase_6	PF13522	91 - 200	2.7e-14	Glutamine amidotransferase domain
GATase_7	PF13537	112 - 202	4e-09	Glutamine amidotransferase domain
SIS	PF01380	365 - 493	2.1e-34	SIS domain
SIS	PF01380	537 - 667	7.3e-18	SIS domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0008285)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G24090
fragaria_vesca	FvH4_6g00360 FvH4_6g00360
malus_domestica	MD04G1173900.v1.1 MD04G1246300.v1.1 MD12G1263800.v1.1
prunus_persica	Prupe.6G362600_v2.0.a1
pyrus_communis	pycom04g21700 pycom12g24000
rosa_chinensis	RchiOBHm_Chr3g0447661 RchiOBHm_Chr4g0412421
rosa_laevigata	RLG00000025980
rosa_multiflora	Rmu_ssc0000391.1_g000002
rosa_roxburghii	Rroxscaffold_6G00425870
rosa_rugosa	Rorug02G0604100 Rorug04G0077300 Rorug07G0328400
rosa_samantha	Rh3AG004100 Rh3BG003700 Rh3CG004000 Rh3DG004000 Rh4CG183700
rosa_wichuraiana	Rw3G000310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	52
Acc36I	ACCTGC	1 cut(s)	52
AccI	GTMKAC	2 cut(s)	228, 1849
AciI	CCGC	6 cut(s)	111, 170, 190, 687, 1386, 1721
AclWI	GGATC	6 cut(s)	140, 700, 1086, 1230, 1599, 2025
AcsI	RAATTY	4 cut(s)	256, 368, 668, 978
AcuI	CTGAAG	5 cut(s)	848, 872, 1094, 1589, 1786
AcyI	GRCGYC	1 cut(s)	85
AdeI	CACNNNGTG	1 cut(s)	303
AfaI	GTAC	3 cut(s)	822, 900, 1132
AfiI	CCNNNNNNNGG	2 cut(s)	196, 1014
AhlI	ACTAGT	1 cut(s)	424
AjnI	CCWGG	2 cut(s)	192, 1370
AleI	CACNNNNGTG	2 cut(s)	1386, 1775
Alw21I	GWGCWC	1 cut(s)	353
Alw26I	GTCTC	1 cut(s)	203
AlwI	GGATC	6 cut(s)	140, 700, 1086, 1230, 1599, 2025
AlwNI	CAGNNNCTG	2 cut(s)	1606, 1978
Ama87I	CYCGRG	1 cut(s)	1895
AoxI	GGCC	4 cut(s)	76, 306, 408, 1692
ApeKI	GCWGC	6 cut(s)	1145, 1154, 1810, 1828, 1880, 1972
ApoI	RAATTY	4 cut(s)	256, 368, 668, 978
ArsI	GACNNNNNNTTYG	2 cut(s)	1520, 1552
Asp700I	GAANNNNTTC	1 cut(s)	471
AspLEI	GCGC	2 cut(s)	295, 1409
AspS9I	GGNCC	5 cut(s)	306, 1232, 1564, 1693, 1739
AsuC2I	CCSGG	5 cut(s)	98, 115, 301, 1896, 1897
AsuII	TTCGAA	1 cut(s)	1868
AsuNHI	GCTAGC	2 cut(s)	683, 1468
AvaI	CYCGRG	1 cut(s)	1895
AvaII	GGWCC	3 cut(s)	1232, 1564, 1739
AxyI	CCTNAGG	1 cut(s)	80
BaeGI	GKGCMC	2 cut(s)	1840, 1895
BaeI	ACNNNNGTAYC	4 cut(s)	453, 486, 1242, 1275
BanII	GRGCYC	1 cut(s)	353
BauI	CACGAG	1 cut(s)	1441
BbsI	GAAGAC	1 cut(s)	1934
Bbv12I	GWGCWC	1 cut(s)	353
BbvCI	CCTCAGC	1 cut(s)	1926
BbvI	GCAGC	6 cut(s)	1132, 1141, 1822, 1840, 1867, 1984
BccI	CCATC	7 cut(s)	348, 803, 827, 919, 1172, 1453, 1995
BciT130I	CCWGG	2 cut(s)	194, 1372
BciVI	GTATCC	1 cut(s)	1637
BcnI	CCSGG	5 cut(s)	98, 115, 301, 1896, 1897
BcoDI	GTCTC	1 cut(s)	203
BcuI	ACTAGT	1 cut(s)	424
BfaI	CTAG	7 cut(s)	425, 684, 1209, 1469, 1475, 1664, 1718
BfmI	CTRYAG	4 cut(s)	59, 771, 1365, 1479
BfoI	RGCGCY	1 cut(s)	1410
BfuAI	ACCTGC	1 cut(s)	52
BfuI	GTATCC	1 cut(s)	1637
BisI	GCNGC	6 cut(s)	1146, 1155, 1811, 1829, 1881, 1973
BlsI	GCNGC	6 cut(s)	1147, 1156, 1812, 1830, 1882, 1974
Bme1390I	CCNGG	7 cut(s)	98, 115, 194, 301, 1372, 1896, 1897
Bme18I	GGWCC	3 cut(s)	1232, 1564, 1739
BmeT110I	CYCGRG	1 cut(s)	1895
BmgT120I	GGNCC	5 cut(s)	306, 1232, 1564, 1693, 1739
BmiI	GGNNCC	1 cut(s)	1039
BmrFI	CCNGG	7 cut(s)	98, 115, 194, 301, 1372, 1896, 1897
BmsI	GCATC	6 cut(s)	577, 751, 902, 1783, 1867, 1892
BmtI	GCTAGC	2 cut(s)	687, 1472
BoxI	GACNNNNGTC	1 cut(s)	215
BpiI	GAAGAC	1 cut(s)	1934
Bpu10I	CCTNAGC	1 cut(s)	1926
Bpu14I	TTCGAA	1 cut(s)	1868
BpuEI	CTTGAG	3 cut(s)	575, 941, 1952
BpuMI	CCSGG	5 cut(s)	98, 115, 301, 1896, 1897
BsaAI	YACGTR	1 cut(s)	1031
BsaBI	GATNNNNATC	1 cut(s)	122
BsaHI	GRCGYC	1 cut(s)	85
BsaI	GGTCTC	1 cut(s)	203
Bsc4I	CCNNNNNNNGG	2 cut(s)	196, 1014
Bse1I	ACTGG	1 cut(s)	756
Bse21I	CCTNAGG	1 cut(s)	80
Bse3DI	GCAATG	1 cut(s)	1967
Bse8I	GATNNNNATC	1 cut(s)	122
BseBI	CCWGG	2 cut(s)	194, 1372
BseGI	GGATG	3 cut(s)	340, 814, 838
BseJI	GATNNNNATC	1 cut(s)	122
BseLI	CCNNNNNNNGG	2 cut(s)	196, 1014
BseMI	GCAATG	1 cut(s)	1967
BseMII	CTCAG	4 cut(s)	71, 1286, 1401, 1940
BseNI	ACTGG	1 cut(s)	756
BseSI	GKGCMC	2 cut(s)	1840, 1895
BseXI	GCAGC	6 cut(s)	1132, 1141, 1822, 1840, 1867, 1984
BshFI	GGCC	4 cut(s)	78, 308, 410, 1694
BsiHKAI	GWGCWC	1 cut(s)	353
BsiHKCI	CYCGRG	1 cut(s)	1895
BsiSI	CCGG	4 cut(s)	97, 114, 301, 1896
BslI	CCNNNNNNNGG	2 cut(s)	196, 1014
BsmAI	GTCTC	1 cut(s)	203
BsnI	GGCC	4 cut(s)	78, 308, 410, 1694
Bso31I	GGTCTC	1 cut(s)	203
BsoBI	CYCGRG	1 cut(s)	1895
Bsp119I	TTCGAA	1 cut(s)	1868
Bsp1286I	GDGCHC	3 cut(s)	353, 1840, 1895
Bsp143I	GATC	6 cut(s)	145, 692, 1078, 1222, 1591, 2017
BspACI	CCGC	6 cut(s)	111, 170, 190, 687, 1386, 1721
BspANI	GGCC	4 cut(s)	78, 308, 410, 1694
BspCNI	CTCAG	4 cut(s)	72, 1287, 1402, 1939
BspHI	TCATGA	1 cut(s)	982
BspLI	GGNNCC	1 cut(s)	1039
BspMAI	CTGCAG	1 cut(s)	63
BspMI	ACCTGC	1 cut(s)	52
BspOI	GCTAGC	2 cut(s)	687, 1472
BspPI	GGATC	6 cut(s)	140, 700, 1086, 1230, 1599, 2025
BspQI	GCTCTTC	1 cut(s)	2033
BspT104I	TTCGAA	1 cut(s)	1868
BspTNI	GGTCTC	1 cut(s)	203
BsrDI	GCAATG	1 cut(s)	1967
BsrI	ACTGG	1 cut(s)	756
BssMI	GATC	6 cut(s)	145, 692, 1078, 1222, 1591, 2017
BssNI	GRCGYC	1 cut(s)	85
BssSI	CACGAG	1 cut(s)	1441
BssT1I	CCWWGG	4 cut(s)	329, 655, 1063, 1431
Bst2BI	CACGAG	1 cut(s)	1441
Bst2UI	CCWGG	2 cut(s)	194, 1372
Bst4CI	ACNGT	6 cut(s)	1057, 1325, 1354, 1532, 1706, 1996
Bst6I	CTCTTC	1 cut(s)	2033
BstACI	GRCGYC	1 cut(s)	85
BstBAI	YACGTR	1 cut(s)	1031
BstBI	TTCGAA	1 cut(s)	1868
BstDEI	CTNAG	5 cut(s)	80, 1295, 1410, 1926, 2052
BstDSI	CCRYGG	1 cut(s)	1950
BstEII	GGTNACC	1 cut(s)	521
BstF5I	GGATG	3 cut(s)	340, 814, 838
BstH2I	RGCGCY	1 cut(s)	1410
BstHHI	GCGC	2 cut(s)	295, 1409
BstKTI	GATC	6 cut(s)	148, 695, 1081, 1225, 1594, 2020
BstMAI	GTCTC	1 cut(s)	203
BstMBI	GATC	6 cut(s)	145, 692, 1078, 1222, 1591, 2017
BstMWI	GCNNNNNNNGC	5 cut(s)	290, 639, 1151, 1154, 1469
BstNI	CCWGG	2 cut(s)	194, 1372
BstNSI	RCATGY	1 cut(s)	737
BstPAI	GACNNNNGTC	1 cut(s)	215
BstPI	GGTNACC	1 cut(s)	521
BstSCI	CCNGG	7 cut(s)	96, 113, 192, 299, 1370, 1894, 1895
BstSFI	CTRYAG	4 cut(s)	59, 771, 1365, 1479
BstSLI	GKGCMC	2 cut(s)	1840, 1895
BstV1I	GCAGC	6 cut(s)	1132, 1141, 1822, 1840, 1867, 1984
BstV2I	GAAGAC	1 cut(s)	1934
BstX2I	RGATCY	1 cut(s)	1591
BstYI	RGATCY	1 cut(s)	1591
Bsu36I	CCTNAGG	1 cut(s)	80
BsuI	GTATCC	1 cut(s)	1637
BsuRI	GGCC	4 cut(s)	78, 308, 410, 1694
BtgI	CCRYGG	1 cut(s)	1950
BtsCI	GGATG	3 cut(s)	340, 814, 838
BtsI	GCAGTG	1 cut(s)	1381
BtsIMutI	CAGTG	4 cut(s)	763, 1381, 1711, 1893
BveI	ACCTGC	1 cut(s)	52
CaiI	CAGNNNCTG	2 cut(s)	1606, 1978
CciI	TCATGA	1 cut(s)	982
CfoI	GCGC	2 cut(s)	295, 1409
Cfr13I	GGNCC	5 cut(s)	306, 1232, 1564, 1693, 1739
Cfr9I	CCCGGG	1 cut(s)	1895
CseI	GACGC	1 cut(s)	74
Csp6I	GTAC	3 cut(s)	821, 899, 1131
CviAII	CATG	6 cut(s)	281, 314, 734, 872, 983, 1736
CviQI	GTAC	3 cut(s)	821, 899, 1131
DdeI	CTNAG	5 cut(s)	80, 1295, 1410, 1926, 2052
DpnI	GATC	6 cut(s)	147, 694, 1080, 1224, 1593, 2019
DpnII	GATC	6 cut(s)	145, 692, 1078, 1222, 1591, 2017
DraI	TTTAAA	2 cut(s)	255, 598
DraIII	CACNNNGTG	1 cut(s)	303
Eam1104I	CTCTTC	1 cut(s)	2033
EarI	CTCTTC	1 cut(s)	2033
EciI	GGCGGA	2 cut(s)	100, 179
Ecl136II	GAGCTC	1 cut(s)	351
Eco130I	CCWWGG	4 cut(s)	329, 655, 1063, 1431
Eco147I	AGGCCT	1 cut(s)	410
Eco24I	GRGCYC	1 cut(s)	353
Eco31I	GGTCTC	1 cut(s)	203
Eco47I	GGWCC	3 cut(s)	1232, 1564, 1739
Eco53kI	GAGCTC	1 cut(s)	351
Eco57I	CTGAAG	5 cut(s)	848, 872, 1094, 1589, 1786
Eco81I	CCTNAGG	1 cut(s)	80
Eco88I	CYCGRG	1 cut(s)	1895
Eco91I	GGTNACC	1 cut(s)	521
EcoICRI	GAGCTC	1 cut(s)	351
EcoO109I	RGGNCCY	2 cut(s)	1232, 1564
EcoO65I	GGTNACC	1 cut(s)	521
EcoRII	CCWGG	2 cut(s)	192, 1370
EcoT14I	CCWWGG	4 cut(s)	329, 655, 1063, 1431
EcoT38I	GRGCYC	1 cut(s)	353
ErhI	CCWWGG	4 cut(s)	329, 655, 1063, 1431
FaeI	CATG	6 cut(s)	284, 317, 737, 875, 986, 1739
FalI	AAGNNNNNCTT	4 cut(s)	537, 569, 1680, 1712
FatI	CATG	6 cut(s)	280, 313, 733, 871, 982, 1735
FauI	CCCGC	2 cut(s)	177, 1714
FauNDI	CATATG	1 cut(s)	583
FblI	GTMKAC	2 cut(s)	228, 1849
Fnu4HI	GCNGC	6 cut(s)	1146, 1155, 1811, 1829, 1881, 1973
FokI	GGATG	3 cut(s)	327, 821, 845
FriOI	GRGCYC	1 cut(s)	353
Fsp4HI	GCNGC	6 cut(s)	1146, 1155, 1811, 1829, 1881, 1973
FspBI	CTAG	7 cut(s)	425, 684, 1209, 1469, 1475, 1664, 1718
GlaI	GCGC	2 cut(s)	294, 1408
GluI	GCNGC	6 cut(s)	1146, 1155, 1811, 1829, 1881, 1973
HaeII	RGCGCY	1 cut(s)	1410
HaeIII	GGCC	4 cut(s)	78, 308, 410, 1694
HapII	CCGG	4 cut(s)	97, 114, 301, 1896
HgaI	GACGC	1 cut(s)	74
HhaI	GCGC	2 cut(s)	295, 1409
Hin1I	GRCGYC	1 cut(s)	85
Hin1II	CATG	6 cut(s)	284, 317, 737, 875, 986, 1739
Hin6I	GCGC	2 cut(s)	293, 1407
HinP1I	GCGC	2 cut(s)	293, 1407
HincII	GTYRAC	2 cut(s)	37, 1850
HindII	GTYRAC	2 cut(s)	37, 1850
HindIII	AAGCTT	2 cut(s)	479, 1571
HpaII	CCGG	4 cut(s)	97, 114, 301, 1896
Hpy166II	GTNNAC	7 cut(s)	37, 229, 382, 731, 878, 1070, 1850
Hpy188I	TCNGA	6 cut(s)	24, 454, 828, 1296, 1559, 2028
Hpy8I	GTNNAC	7 cut(s)	37, 229, 382, 731, 878, 1070, 1850
HpyCH4III	ACNGT	6 cut(s)	1057, 1325, 1354, 1532, 1706, 1996
HpyCH4IV	ACGT	3 cut(s)	33, 1030, 2013
HpyF10VI	GCNNNNNNNGC	5 cut(s)	290, 639, 1151, 1154, 1469
HpyF3I	CTNAG	5 cut(s)	80, 1295, 1410, 1926, 2052
HpySE526I	ACGT	3 cut(s)	33, 1030, 2013
Hsp92I	GRCGYC	1 cut(s)	85
Hsp92II	CATG	6 cut(s)	284, 317, 737, 875, 986, 1739
HspAI	GCGC	2 cut(s)	293, 1407
Kzo9I	GATC	6 cut(s)	145, 692, 1078, 1222, 1591, 2017
LguI	GCTCTTC	1 cut(s)	2033
LmnI	GCTCC	2 cut(s)	331, 657
Lsp1109I	GCAGC	6 cut(s)	1132, 1141, 1822, 1840, 1867, 1984
LweI	GCATC	6 cut(s)	577, 751, 902, 1783, 1867, 1892
MaeI	CTAG	7 cut(s)	425, 684, 1209, 1469, 1475, 1664, 1718
MaeII	ACGT	3 cut(s)	33, 1030, 2013
MaeIII	GTNAC	7 cut(s)	50, 521, 953, 1192, 1487, 1620, 2044
MalI	GATC	6 cut(s)	147, 694, 1080, 1224, 1593, 2019
MboI	GATC	6 cut(s)	145, 692, 1078, 1222, 1591, 2017
MboII	GAAGA	6 cut(s)	156, 275, 1184, 1259, 1934, 2050
MflI	RGATCY	1 cut(s)	1591
MhlI	GDGCHC	3 cut(s)	353, 1840, 1895
MlyI	GAGTC	3 cut(s)	399, 1453, 1845
MmeI	TCCRAC	1 cut(s)	1200
MroXI	GAANNNNTTC	1 cut(s)	471
MseI	TTAA	8 cut(s)	254, 413, 597, 672, 1086, 1956, 2000, 2010
MslI	CAYNNNNRTG	5 cut(s)	1386, 1398, 1775, 1859, 1888
MspA1I	CMGCKG	1 cut(s)	1256
MspI	CCGG	4 cut(s)	97, 114, 301, 1896
MspR9I	CCNGG	7 cut(s)	98, 115, 194, 301, 1372, 1896, 1897
MvaI	CCWGG	2 cut(s)	194, 1372
MwoI	GCNNNNNNNGC	5 cut(s)	290, 639, 1151, 1154, 1469
NciI	CCSGG	5 cut(s)	98, 115, 301, 1896, 1897
NdeI	CATATG	1 cut(s)	583
NdeII	GATC	6 cut(s)	145, 692, 1078, 1222, 1591, 2017
NheI	GCTAGC	2 cut(s)	683, 1468
NlaIII	CATG	6 cut(s)	284, 317, 737, 875, 986, 1739
NlaIV	GGNNCC	1 cut(s)	1039
NmuCI	GTSAC	5 cut(s)	521, 1192, 1487, 1620, 2044
NspI	RCATGY	1 cut(s)	737
NspV	TTCGAA	1 cut(s)	1868
OliI	CACNNNNGTG	2 cut(s)	1386, 1775
PagI	TCATGA	1 cut(s)	982
PaqCI	CACCTGC	1 cut(s)	52
PceI	AGGCCT	1 cut(s)	410
PciSI	GCTCTTC	1 cut(s)	2033
PcsI	WCGNNNNNNNCGW	2 cut(s)	213, 222
PdmI	GAANNNNTTC	1 cut(s)	471
PfeI	GAWTC	7 cut(s)	107, 266, 419, 438, 449, 995, 2029
PkrI	GCNGC	6 cut(s)	1147, 1156, 1812, 1830, 1882, 1974
PleI	GAGTC	3 cut(s)	398, 1452, 1845
PpsI	GAGTC	3 cut(s)	398, 1452, 1845
Ppu21I	YACGTR	1 cut(s)	1031
PpuMI	RGGWCCY	2 cut(s)	1232, 1564
PshAI	GACNNNNGTC	1 cut(s)	215
Psp124BI	GAGCTC	1 cut(s)	353
Psp5II	RGGWCCY	2 cut(s)	1232, 1564
Psp6I	CCWGG	2 cut(s)	192, 1370
PspEI	GGTNACC	1 cut(s)	521
PspGI	CCWGG	2 cut(s)	192, 1370
PspN4I	GGNNCC	1 cut(s)	1039
PspPI	GGNCC	5 cut(s)	306, 1232, 1564, 1693, 1739
PspPPI	RGGWCCY	2 cut(s)	1232, 1564
PstI	CTGCAG	1 cut(s)	63
PstNI	CAGNNNCTG	2 cut(s)	1606, 1978
PsuI	RGATCY	1 cut(s)	1591
PvuII	CAGCTG	1 cut(s)	1256
RsaI	GTAC	3 cut(s)	822, 900, 1132
RsaNI	GTAC	3 cut(s)	821, 899, 1131
RseI	CAYNNNNRTG	5 cut(s)	1386, 1398, 1775, 1859, 1888
SacI	GAGCTC	1 cut(s)	353
SalI	GTCGAC	1 cut(s)	1848
SapI	GCTCTTC	1 cut(s)	2033
SaqAI	TTAA	8 cut(s)	254, 413, 597, 672, 1086, 1956, 2000, 2010
SatI	GCNGC	6 cut(s)	1146, 1155, 1811, 1829, 1881, 1973
Sau3AI	GATC	6 cut(s)	145, 692, 1078, 1222, 1591, 2017
Sau96I	GGNCC	5 cut(s)	306, 1232, 1564, 1693, 1739
SchI	GAGTC	3 cut(s)	399, 1453, 1845
ScrFI	CCNGG	7 cut(s)	98, 115, 194, 301, 1372, 1896, 1897
SduI	GDGCHC	3 cut(s)	353, 1840, 1895
SfaNI	GCATC	6 cut(s)	577, 751, 902, 1783, 1867, 1892
SfcI	CTRYAG	4 cut(s)	59, 771, 1365, 1479
SfuI	TTCGAA	1 cut(s)	1868
SinI	GGWCC	3 cut(s)	1232, 1564, 1739
SmaI	CCCGGG	1 cut(s)	1897
SmiI	ATTTAAAT	1 cut(s)	255
SmiMI	CAYNNNNRTG	5 cut(s)	1386, 1398, 1775, 1859, 1888
SmlI	CTYRAG	3 cut(s)	554, 920, 1931
SmoI	CTYRAG	3 cut(s)	554, 920, 1931
SpeI	ACTAGT	1 cut(s)	424
SseBI	AGGCCT	1 cut(s)	410
SsiI	CCGC	6 cut(s)	111, 170, 190, 687, 1386, 1721
SspI	AATATT	1 cut(s)	1960
SspMI	CTAG	7 cut(s)	425, 684, 1209, 1469, 1475, 1664, 1718
SstI	GAGCTC	1 cut(s)	353
StuI	AGGCCT	1 cut(s)	410
StyD4I	CCNGG	7 cut(s)	96, 113, 192, 299, 1370, 1894, 1895
StyI	CCWWGG	4 cut(s)	329, 655, 1063, 1431
SwaI	ATTTAAAT	1 cut(s)	255
TaaI	ACNGT	6 cut(s)	1057, 1325, 1354, 1532, 1706, 1996
TaiI	ACGT	3 cut(s)	36, 1033, 2016
TaqI	TCGA	6 cut(s)	126, 144, 207, 1849, 1868, 1909
TatI	WGTACW	1 cut(s)	898
TfiI	GAWTC	7 cut(s)	107, 266, 419, 438, 449, 995, 2029
Tru1I	TTAA	8 cut(s)	254, 413, 597, 672, 1086, 1956, 2000, 2010
Tru9I	TTAA	8 cut(s)	254, 413, 597, 672, 1086, 1956, 2000, 2010
TscAI	CASTG	4 cut(s)	763, 1388, 1711, 1893
TseFI	GTSAC	5 cut(s)	521, 1192, 1487, 1620, 2044
TseI	GCWGC	6 cut(s)	1145, 1154, 1810, 1828, 1880, 1972
Tsp45I	GTSAC	5 cut(s)	521, 1192, 1487, 1620, 2044
TspDTI	ATGAA	8 cut(s)	381, 971, 999, 1108, 1382, 1601, 1745, 1773
TspGWI	ACGGA	3 cut(s)	214, 450, 636
TspMI	CCCGGG	1 cut(s)	1895
TspRI	CASTG	4 cut(s)	763, 1388, 1711, 1893
VpaK11BI	GGWCC	3 cut(s)	1232, 1564, 1739
XapI	RAATTY	4 cut(s)	256, 368, 668, 978
XbaI	TCTAGA	1 cut(s)	1663
XceI	RCATGY	1 cut(s)	737
XcmI	CCANNNNNNNNNTGG	1 cut(s)	922
XmaI	CCCGGG	1 cut(s)	1895
XmiI	GTMKAC	2 cut(s)	228, 1849
XmnI	GAANNNNTTC	1 cut(s)	471
XspI	CTAG	7 cut(s)	425, 684, 1209, 1469, 1475, 1664, 1718

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.