Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0008285

Physical Location & Seq

Genomic Coordinates Reverse (-)

33017291 .. 33022123

Gene Structure 4833 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1263800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2058 bp

ATGTGTGGGATATTTGCGTATCTGAATTACGACGTCAACAGAGAGAGGCGCTACATTCTTCAGGTGCTTTTCAATGGCCTGAGGCGTTTGGAATACCGGGGATACGATTCCGCCGGGATTTCCATCGATCATTCTTATTCCTTCGATCCAAACACCCCCCAATCTTCCCCGCTTCACCCCCTCGTTTTCCGCCAGGAGGGAAACATCGAGACCCTCGTCAAATCCGTATACCAAGAGGTTGCTGAAACAGATTTAAATTTGGAAGAATCTTTCTCTCACCATGCTGGAATAGCACACACCCGATGGGCCACCCATGGAGAGCCAGCTCCAAGGAACAGCCATCCACAGAGCTCTGGTTCTGGAAATGAATTTTTGGTTGTTCACAATGGAGTCATCACTAATTATGAGGCCTTAAAGGAATCACTAGTTCGGCACGGATTCACCTTTGAATCTGAAACAGACACAGAAGTAATTCCAAAGCTTGCCAAATATGTTTATGATAAAGCTAAGGAAGGAGAAGGTGACCAGACTATCACATTTAGTCAAGTTGTGCTTGAGGTTATGAGGCATCTTGAAGGTGCATATGCCCTGATTTTTAAAAGCCGGCATTATCCAAATGAGTTGATTGCTTGCAAGCGTGGCAGTCCGTTGCTCCTTGGTGTCAGAGAATTTAACGAAAATGCTAGCGGTGGATCTGCATTTCACGATGATAATTTCCTTTCAAAAAGTGGACATCCCAAAGAACTCTTTTTATCCAGTGATGCAAATGCTGTAGTTGAACACACTAAAAAGGTTTTGGTGATTGAGGATGGTGAAGTGGTACATCTGAAGGATGGAGGTGTTTCTATCATGAAGTTTGATAAAGGACAGCATGGTGGCCTTTCAAGGGTTGCATCTGTACAACGAGCTTTGTCCATTCTTGAGATGGAGGTTGAACAAATAAACAAAGGGAGTTACAAACATTATATGCAGAAAGAAATTCATGAACAGCCTGAATCTTTAACTACCACAATGAGGGGGAGACTTTTACGTGGAGGTTCCTGCAAAGCCAAGACTGTTCTCCTAGGTGGACTGAAGGATCACCTTAAAACAATTAGGCGAAGCAGGCGAATTGTTTTCATCGGTTGTGGTACAAGCTACAATGCTGCTTTAGCTGCAAGACCTATTTTGGAAGAACTTTCTGGTATTCCTGTCACAATGGAAATTGCTAGTGATTTGTTGGATCGGCAAGGACCTATATACAGAGAAGATACAGCTGTCTTTGTCAGTCAGTCTGGTGAAACAGCAGATACTCTGAGTGCTTTGGAATATGCGTTGGAAAATGGTGCATTATGCGTTGGCATAACAAATACTGTTGGTAGTGCTATAGCCAGGAATACACACTGCGGTGTTCATATAAATGCTGGCGCTGAGATTGGGGTGGCTAGCACCAAGGCATACACGAGTCAAATAGTGGTGATGGCTATGCTAGCACTAGCTATAGGAGGTGACACAATTTCTAATCAAGCACGAAGAGAGGCTATAATTGACGGTTTATTTGAGTTACCAAACAAAGTCAGAGAGGTCCTGAAGCTTGACGGGATGATGAAGGATCTTGCACAGGAACTGATAGCTCAGCAGTCACTTCTTGTGTTTGGGAGAGGATACAACTACGCAACATCTCTAGAGGGTGCTTTGAAGGTAAAGGAAGTGGCCCTTATGCACAGCGAAGGAATACTAGCCGGTGAAATGAAACATGGCCCTTTGGCTTTAGTTGATGAAACTCTCCCTACACTTGTGATTGCCACCCGTGATGCTTGCTTCAGTAAGCAGCAGTCAGTTATTCAGCAGCTTCATGCGCGCAAAGGTCGACTCATAGTAATGTGTTCAAAAGGAGATGCTGCATCAGTGTGCCCTGGGGGGACTTGTCGAGTGATTGAAGTTCCTCAGCTTGAGGATTGTCTTCAACCCGTAGTTAACATTGTTCCATTGCAGCTGCTGGCATACCATCTCACTGTTTTAAGAGGTTTCAATGTGGATCAGCCTCGGAATCTTGCAAAGAGCGTGACTACGCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004360 GO:0006002 GO:0006040 GO:0006047 GO:0006139 GO:0006464 GO:0006486 GO:0006487 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008483 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009225 GO:0009987 GO:0016740 GO:0016769 GO:0019538 GO:0019637 GO:0034641 GO:0034645 GO:0036211 GO:0043170 GO:0043412 GO:0043413 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0046483 GO:0055086 GO:0070085 GO:0070548 GO:0071704 GO:1901135 GO:1901137 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00250 ko00520 ko01100 ko01130 ko04931 map00250 map00520 map01100 map01130 map04931

Pfam Domains

Protein Families

GATase_6 SIS

Protein Analysis

686

Amino Acids

75.12

Weight (kDa)

6.28

Isoelectric Point (pI)

41.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GATase_6	PF13522	91 - 202	2e-14	Glutamine amidotransferase domain
GATase_7	PF13537	112 - 203	3.8e-09	Glutamine amidotransferase domain
SIS	PF01380	365 - 493	2.1e-34	SIS domain
SIS	PF01380	536 - 667	2.2e-17	SIS domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008285)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G24090
fragaria_vesca	FvH4_6g00360 FvH4_6g00360
malus_domestica	MD04G1173900.v1.1 MD04G1246300.v1.1 MD12G1263800.v1.1
prunus_persica	Prupe.6G362600_v2.0.a1
pyrus_communis	pycom04g21700 pycom12g24000
rosa_chinensis	RchiOBHm_Chr3g0447661 RchiOBHm_Chr4g0412421
rosa_laevigata	RLG00000025980
rosa_multiflora	Rmu_ssc0000391.1_g000002
rosa_roxburghii	Rroxscaffold_6G00425870
rosa_rugosa	Rorug02G0604100 Rorug04G0077300 Rorug07G0328400
rosa_samantha	Rh3AG004100 Rh3BG003700 Rh3CG004000 Rh3DG004000 Rh4CG183700
rosa_wichuraiana	Rw3G000310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	36
AccI	GTMKAC	2 cut(s)	228, 1849
AccII	CGCG	1 cut(s)	1840
AciI	CCGC	5 cut(s)	111, 170, 190, 687, 1386
AclWI	GGATC	6 cut(s)	140, 700, 1086, 1230, 1599, 2025
AcsI	RAATTY	4 cut(s)	256, 368, 668, 978
AcuI	CTGAAG	5 cut(s)	44, 848, 1094, 1589, 1786
AcyI	GRCGYC	1 cut(s)	33
AfaI	GTAC	3 cut(s)	822, 900, 1132
AfiI	CCNNNNNNNGG	3 cut(s)	196, 886, 1014
AhlI	ACTAGT	1 cut(s)	424
AjnI	CCWGG	3 cut(s)	192, 1370, 1894
AleI	CACNNNNGTG	1 cut(s)	1386
Alw21I	GWGCWC	1 cut(s)	353
Alw26I	GTCTC	2 cut(s)	203, 1015
AlwI	GGATC	6 cut(s)	140, 700, 1086, 1230, 1599, 2025
AlwNI	CAGNNNCTG	2 cut(s)	1606, 1978
AoxI	GGCC	6 cut(s)	76, 306, 408, 877, 1692, 1738
ApeKI	GCWGC	7 cut(s)	1145, 1154, 1810, 1828, 1880, 1972, 1975
ApoI	RAATTY	4 cut(s)	256, 368, 668, 978
ArsI	GACNNNNNNTTYG	2 cut(s)	1520, 1552
Asp700I	GAANNNNTTC	1 cut(s)	471
AspA2I	CCTAGG	1 cut(s)	1063
AspLEI	GCGC	4 cut(s)	51, 1409, 1840, 1842
AspS9I	GGNCC	5 cut(s)	306, 1232, 1564, 1693, 1739
AsuC2I	CCSGG	2 cut(s)	98, 115
AsuNHI	GCTAGC	3 cut(s)	683, 1424, 1468
AvaII	GGWCC	2 cut(s)	1232, 1564
AvrII	CCTAGG	1 cut(s)	1063
AxyI	CCTNAGG	1 cut(s)	80
BaeGI	GKGCMC	1 cut(s)	1895
BaeI	ACNNNNGTAYC	2 cut(s)	1242, 1275
BanII	GRGCYC	1 cut(s)	353
BauI	CACGAG	1 cut(s)	1441
BbsI	GAAGAC	1 cut(s)	1934
Bbv12I	GWGCWC	1 cut(s)	353
BbvCI	CCTCAGC	1 cut(s)	1926
BbvI	GCAGC	7 cut(s)	1132, 1141, 1822, 1840, 1867, 1962, 1984
BccI	CCATC	8 cut(s)	131, 297, 348, 803, 827, 919, 1453, 1995
BciT130I	CCWGG	3 cut(s)	194, 1372, 1896
BciVI	GTATCC	2 cut(s)	95, 1637
BcnI	CCSGG	2 cut(s)	98, 115
BcoDI	GTCTC	2 cut(s)	203, 1015
BcuI	ACTAGT	1 cut(s)	424
BfaI	CTAG	9 cut(s)	425, 684, 1064, 1209, 1425, 1469, 1475, 1664, 1718
BfmI	CTRYAG	3 cut(s)	771, 1365, 1479
BfoI	RGCGCY	2 cut(s)	52, 1410
BfuI	GTATCC	2 cut(s)	95, 1637
BisI	GCNGC	7 cut(s)	1146, 1155, 1811, 1829, 1881, 1973, 1976
BlnI	CCTAGG	1 cut(s)	1063
BlpI	GCTNAGC	1 cut(s)	1614
BlsI	GCNGC	7 cut(s)	1147, 1156, 1812, 1830, 1882, 1974, 1977
Bme1390I	CCNGG	5 cut(s)	98, 115, 194, 1372, 1896
Bme18I	GGWCC	2 cut(s)	1232, 1564
BmgT120I	GGNCC	5 cut(s)	306, 1232, 1564, 1693, 1739
BmiI	GGNNCC	1 cut(s)	1039
BmrFI	CCNGG	5 cut(s)	98, 115, 194, 1372, 1896
BmsI	GCATC	6 cut(s)	577, 751, 902, 1783, 1867, 1892
BmtI	GCTAGC	3 cut(s)	687, 1428, 1472
BoxI	GACNNNNGTC	1 cut(s)	215
BpiI	GAAGAC	1 cut(s)	1934
Bpu10I	CCTNAGC	2 cut(s)	507, 1926
Bpu1102I	GCTNAGC	1 cut(s)	1614
BpuEI	CTTGAG	3 cut(s)	575, 941, 1952
BpuMI	CCSGG	2 cut(s)	98, 115
Bsa29I	ATCGAT	1 cut(s)	126
BsaAI	YACGTR	1 cut(s)	1031
BsaBI	GATNNNNATC	1 cut(s)	122
BsaHI	GRCGYC	1 cut(s)	33
BsaI	GGTCTC	1 cut(s)	203
BsaJI	CCNNGG	9 cut(s)	97, 313, 329, 655, 1063, 1431, 1894, 1895, 2024
Bsc4I	CCNNNNNNNGG	3 cut(s)	196, 886, 1014
Bse118I	RCCGGY	2 cut(s)	603, 1721
Bse1I	ACTGG	1 cut(s)	756
Bse21I	CCTNAGG	1 cut(s)	80
Bse3DI	GCAATG	1 cut(s)	1967
Bse8I	GATNNNNATC	1 cut(s)	122
BseBI	CCWGG	3 cut(s)	194, 1372, 1896
BseCI	ATCGAT	1 cut(s)	126
BseDI	CCNNGG	9 cut(s)	97, 313, 329, 655, 1063, 1431, 1894, 1895, 2024
BseGI	GGATG	5 cut(s)	340, 733, 814, 838, 1587
BseJI	GATNNNNATC	1 cut(s)	122
BseLI	CCNNNNNNNGG	3 cut(s)	196, 886, 1014
BseMI	GCAATG	1 cut(s)	1967
BseMII	CTCAG	5 cut(s)	71, 1286, 1401, 1628, 1940
BseNI	ACTGG	1 cut(s)	756
BsePI	GCGCGC	1 cut(s)	1838
BseSI	GKGCMC	1 cut(s)	1895
BseXI	GCAGC	7 cut(s)	1132, 1141, 1822, 1840, 1867, 1962, 1984
Bsh1236I	CGCG	1 cut(s)	1840
BshFI	GGCC	6 cut(s)	78, 308, 410, 879, 1694, 1740
BshVI	ATCGAT	1 cut(s)	126
BsiHKAI	GWGCWC	1 cut(s)	353
BsiSI	CCGG	4 cut(s)	97, 114, 604, 1722
BslFI	GGGAC	1 cut(s)	1915
BslI	CCNNNNNNNGG	3 cut(s)	196, 886, 1014
BsmAI	GTCTC	2 cut(s)	203, 1015
BsmFI	GGGAC	1 cut(s)	1915
BsnI	GGCC	6 cut(s)	78, 308, 410, 879, 1694, 1740
Bso31I	GGTCTC	1 cut(s)	203
Bsp1286I	GDGCHC	2 cut(s)	353, 1895
Bsp1407I	TGTACA	1 cut(s)	898
Bsp143I	GATC	7 cut(s)	127, 145, 692, 1078, 1222, 1591, 2017
Bsp1720I	GCTNAGC	1 cut(s)	1614
Bsp19I	CCATGG	1 cut(s)	313
BspACI	CCGC	5 cut(s)	111, 170, 190, 687, 1386
BspANI	GGCC	6 cut(s)	78, 308, 410, 879, 1694, 1740
BspCNI	CTCAG	5 cut(s)	72, 1287, 1402, 1627, 1939
BspDI	ATCGAT	1 cut(s)	126
BspFNI	CGCG	1 cut(s)	1840
BspHI	TCATGA	2 cut(s)	849, 982
BspLI	GGNNCC	1 cut(s)	1039
BspOI	GCTAGC	3 cut(s)	687, 1428, 1472
BspPI	GGATC	6 cut(s)	140, 700, 1086, 1230, 1599, 2025
BspTNI	GGTCTC	1 cut(s)	203
BsrDI	GCAATG	1 cut(s)	1967
BsrFI	RCCGGY	2 cut(s)	603, 1721
BsrGI	TGTACA	1 cut(s)	898
BsrI	ACTGG	1 cut(s)	756
BssAI	RCCGGY	2 cut(s)	603, 1721
BssECI	CCNNGG	9 cut(s)	97, 313, 329, 655, 1063, 1431, 1894, 1895, 2024
BssHII	GCGCGC	1 cut(s)	1838
BssMI	GATC	7 cut(s)	127, 145, 692, 1078, 1222, 1591, 2017
BssNAI	GTATAC	1 cut(s)	229
BssNI	GRCGYC	1 cut(s)	33
BssSI	CACGAG	1 cut(s)	1441
BssT1I	CCWWGG	5 cut(s)	313, 329, 655, 1063, 1431
Bst1107I	GTATAC	1 cut(s)	229
Bst2BI	CACGAG	1 cut(s)	1441
Bst2UI	CCWGG	3 cut(s)	194, 1372, 1896
Bst4CI	ACNGT	4 cut(s)	1057, 1354, 1532, 1996
Bst6I	CTCTTC	1 cut(s)	1507
BstACI	GRCGYC	1 cut(s)	33
BstAUI	TGTACA	1 cut(s)	898
BstBAI	YACGTR	1 cut(s)	1031
BstDEI	CTNAG	6 cut(s)	80, 507, 1295, 1410, 1614, 1926
BstDSI	CCRYGG	1 cut(s)	313
BstEII	GGTNACC	1 cut(s)	521
BstF5I	GGATG	5 cut(s)	340, 733, 814, 838, 1587
BstFNI	CGCG	1 cut(s)	1840
BstH2I	RGCGCY	2 cut(s)	52, 1410
BstHHI	GCGC	4 cut(s)	51, 1409, 1840, 1842
BstKTI	GATC	7 cut(s)	130, 148, 695, 1081, 1225, 1594, 2020
BstMAI	GTCTC	2 cut(s)	203, 1015
BstMBI	GATC	7 cut(s)	127, 145, 692, 1078, 1222, 1591, 2017
BstMWI	GCNNNNNNNGC	7 cut(s)	290, 639, 1105, 1151, 1154, 1469, 1837
BstNI	CCWGG	3 cut(s)	194, 1372, 1896
BstPAI	GACNNNNGTC	1 cut(s)	215
BstPI	GGTNACC	1 cut(s)	521
BstSCI	CCNGG	5 cut(s)	96, 113, 192, 1370, 1894
BstSFI	CTRYAG	3 cut(s)	771, 1365, 1479
BstSLI	GKGCMC	1 cut(s)	1895
BstUI	CGCG	1 cut(s)	1840
BstV1I	GCAGC	7 cut(s)	1132, 1141, 1822, 1840, 1867, 1962, 1984
BstV2I	GAAGAC	1 cut(s)	1934
BstX2I	RGATCY	2 cut(s)	692, 1591
BstYI	RGATCY	2 cut(s)	692, 1591
BstZ17I	GTATAC	1 cut(s)	229
Bsu15I	ATCGAT	1 cut(s)	126
Bsu36I	CCTNAGG	1 cut(s)	80
BsuI	GTATCC	2 cut(s)	95, 1637
BsuRI	GGCC	6 cut(s)	78, 308, 410, 879, 1694, 1740
BsuTUI	ATCGAT	1 cut(s)	126
BtgI	CCRYGG	1 cut(s)	313
BtsCI	GGATG	5 cut(s)	340, 733, 814, 838, 1587
BtsI	GCAGTG	1 cut(s)	1381
BtsIMutI	CAGTG	4 cut(s)	763, 1381, 1893, 1992
CaiI	CAGNNNCTG	2 cut(s)	1606, 1978
CciI	TCATGA	2 cut(s)	849, 982
CfoI	GCGC	4 cut(s)	51, 1409, 1840, 1842
Cfr10I	RCCGGY	2 cut(s)	603, 1721
Cfr13I	GGNCC	5 cut(s)	306, 1232, 1564, 1693, 1739
ClaI	ATCGAT	1 cut(s)	126
Csp6I	GTAC	3 cut(s)	821, 899, 1131
CviAII	CATG	7 cut(s)	281, 314, 850, 872, 983, 1736, 1835
CviQI	GTAC	3 cut(s)	821, 899, 1131
DdeI	CTNAG	6 cut(s)	80, 507, 1295, 1410, 1614, 1926
DpnI	GATC	7 cut(s)	129, 147, 694, 1080, 1224, 1593, 2019
DpnII	GATC	7 cut(s)	127, 145, 692, 1078, 1222, 1591, 2017
DraI	TTTAAA	2 cut(s)	255, 598
Eam1104I	CTCTTC	1 cut(s)	1507
EarI	CTCTTC	1 cut(s)	1507
EciI	GGCGGA	2 cut(s)	100, 179
Ecl136II	GAGCTC	1 cut(s)	351
Eco130I	CCWWGG	5 cut(s)	313, 329, 655, 1063, 1431
Eco147I	AGGCCT	1 cut(s)	410
Eco24I	GRGCYC	1 cut(s)	353
Eco31I	GGTCTC	1 cut(s)	203
Eco47I	GGWCC	2 cut(s)	1232, 1564
Eco53kI	GAGCTC	1 cut(s)	351
Eco57I	CTGAAG	5 cut(s)	44, 848, 1094, 1589, 1786
Eco81I	CCTNAGG	1 cut(s)	80
Eco91I	GGTNACC	1 cut(s)	521
EcoICRI	GAGCTC	1 cut(s)	351
EcoO109I	RGGNCCY	2 cut(s)	1232, 1564
EcoO65I	GGTNACC	1 cut(s)	521
EcoRII	CCWGG	3 cut(s)	192, 1370, 1894
EcoT14I	CCWWGG	5 cut(s)	313, 329, 655, 1063, 1431
EcoT38I	GRGCYC	1 cut(s)	353
ErhI	CCWWGG	5 cut(s)	313, 329, 655, 1063, 1431
FaeI	CATG	7 cut(s)	284, 317, 853, 875, 986, 1739, 1838
FalI	AAGNNNNNCTT	4 cut(s)	537, 569, 1680, 1712
FaqI	GGGAC	1 cut(s)	1915
FatI	CATG	7 cut(s)	280, 313, 849, 871, 982, 1735, 1834
FauI	CCCGC	1 cut(s)	177
FauNDI	CATATG	1 cut(s)	583
FblI	GTMKAC	2 cut(s)	228, 1849
Fnu4HI	GCNGC	7 cut(s)	1146, 1155, 1811, 1829, 1881, 1973, 1976
FokI	GGATG	5 cut(s)	327, 720, 821, 845, 1594
FriOI	GRGCYC	1 cut(s)	353
Fsp4HI	GCNGC	7 cut(s)	1146, 1155, 1811, 1829, 1881, 1973, 1976
FspBI	CTAG	9 cut(s)	425, 684, 1064, 1209, 1425, 1469, 1475, 1664, 1718
GlaI	GCGC	4 cut(s)	50, 1408, 1839, 1841
GluI	GCNGC	7 cut(s)	1146, 1155, 1811, 1829, 1881, 1973, 1976
HaeII	RGCGCY	2 cut(s)	52, 1410
HaeIII	GGCC	6 cut(s)	78, 308, 410, 879, 1694, 1740
HapII	CCGG	4 cut(s)	97, 114, 604, 1722
HhaI	GCGC	4 cut(s)	51, 1409, 1840, 1842
Hin1I	GRCGYC	1 cut(s)	33
Hin1II	CATG	7 cut(s)	284, 317, 853, 875, 986, 1739, 1838
Hin6I	GCGC	4 cut(s)	49, 1407, 1838, 1840
HinP1I	GCGC	4 cut(s)	49, 1407, 1838, 1840
HincII	GTYRAC	3 cut(s)	37, 1850, 1957
HindII	GTYRAC	3 cut(s)	37, 1850, 1957
HindIII	AAGCTT	2 cut(s)	479, 1571
HpaI	GTTAAC	1 cut(s)	1957
HpaII	CCGG	4 cut(s)	97, 114, 604, 1722
Hpy166II	GTNNAC	7 cut(s)	37, 229, 382, 731, 1070, 1850, 1957
Hpy188I	TCNGA	7 cut(s)	24, 454, 665, 828, 1296, 1559, 2028
Hpy188III	TCNNGA	9 cut(s)	208, 360, 572, 704, 850, 920, 983, 1567, 1664
Hpy8I	GTNNAC	7 cut(s)	37, 229, 382, 731, 1070, 1850, 1957
Hpy99I	CGWCG	1 cut(s)	35
HpyAV	CCTTC	9 cut(s)	151, 506, 512, 569, 823, 1069, 1582, 1672, 1703
HpyCH4III	ACNGT	4 cut(s)	1057, 1354, 1532, 1996
HpyCH4IV	ACGT	2 cut(s)	33, 1030
HpyF10VI	GCNNNNNNNGC	7 cut(s)	290, 639, 1105, 1151, 1154, 1469, 1837
HpyF3I	CTNAG	6 cut(s)	80, 507, 1295, 1410, 1614, 1926
HpySE526I	ACGT	2 cut(s)	33, 1030
Hsp92I	GRCGYC	1 cut(s)	33
Hsp92II	CATG	7 cut(s)	284, 317, 853, 875, 986, 1739, 1838
HspAI	GCGC	4 cut(s)	49, 1407, 1838, 1840
KroI	GCCGGC	1 cut(s)	603
KroNI	GCCGGC	1 cut(s)	605
KspAI	GTTAAC	1 cut(s)	1957
Kzo9I	GATC	7 cut(s)	127, 145, 692, 1078, 1222, 1591, 2017
LmnI	GCTCC	2 cut(s)	331, 657
Lsp1109I	GCAGC	7 cut(s)	1132, 1141, 1822, 1840, 1867, 1962, 1984
LweI	GCATC	6 cut(s)	577, 751, 902, 1783, 1867, 1892
MaeI	CTAG	9 cut(s)	425, 684, 1064, 1209, 1425, 1469, 1475, 1664, 1718
MaeII	ACGT	2 cut(s)	33, 1030
MaeIII	GTNAC	7 cut(s)	521, 953, 1192, 1487, 1542, 1620, 2044
MalI	GATC	7 cut(s)	129, 147, 694, 1080, 1224, 1593, 2019
MboI	GATC	7 cut(s)	127, 145, 692, 1078, 1222, 1591, 2017
MboII	GAAGA	7 cut(s)	50, 156, 275, 1184, 1259, 1524, 1934
MflI	RGATCY	2 cut(s)	692, 1591
MhlI	GDGCHC	2 cut(s)	353, 1895
MlyI	GAGTC	3 cut(s)	399, 1453, 1845
MmeI	TCCRAC	2 cut(s)	1200, 1296
MroNI	GCCGGC	1 cut(s)	603
MroXI	GAANNNNTTC	1 cut(s)	471
MseI	TTAA	8 cut(s)	254, 413, 597, 672, 1001, 1086, 1956, 2000
MslI	CAYNNNNRTG	4 cut(s)	1386, 1398, 1859, 1888
MspA1I	CMGCKG	2 cut(s)	1256, 1975
MspI	CCGG	4 cut(s)	97, 114, 604, 1722
MspR9I	CCNGG	5 cut(s)	98, 115, 194, 1372, 1896
MvaI	CCWGG	3 cut(s)	194, 1372, 1896
MvnI	CGCG	1 cut(s)	1840
MwoI	GCNNNNNNNGC	7 cut(s)	290, 639, 1105, 1151, 1154, 1469, 1837
NaeI	GCCGGC	1 cut(s)	605
NciI	CCSGG	2 cut(s)	98, 115
NcoI	CCATGG	1 cut(s)	313
NdeI	CATATG	1 cut(s)	583
NdeII	GATC	7 cut(s)	127, 145, 692, 1078, 1222, 1591, 2017
NgoMIV	GCCGGC	1 cut(s)	603
NheI	GCTAGC	3 cut(s)	683, 1424, 1468
NlaIII	CATG	7 cut(s)	284, 317, 853, 875, 986, 1739, 1838
NlaIV	GGNNCC	1 cut(s)	1039
NmuCI	GTSAC	5 cut(s)	521, 1192, 1487, 1620, 2044
OliI	CACNNNNGTG	1 cut(s)	1386
PagI	TCATGA	2 cut(s)	849, 982
PasI	CCCWGGG	1 cut(s)	1895
PauI	GCGCGC	1 cut(s)	1838
PceI	AGGCCT	1 cut(s)	410
PcsI	WCGNNNNNNNCGW	2 cut(s)	213, 222
PdiI	GCCGGC	1 cut(s)	605
PdmI	GAANNNNTTC	1 cut(s)	471
PfeI	GAWTC	7 cut(s)	107, 266, 419, 438, 449, 995, 2029
PkrI	GCNGC	7 cut(s)	1147, 1156, 1812, 1830, 1882, 1974, 1977
PleI	GAGTC	3 cut(s)	398, 1452, 1845
PpsI	GAGTC	3 cut(s)	398, 1452, 1845
Ppu21I	YACGTR	1 cut(s)	1031
PpuMI	RGGWCCY	2 cut(s)	1232, 1564
PshAI	GACNNNNGTC	1 cut(s)	215
Psp124BI	GAGCTC	1 cut(s)	353
Psp5II	RGGWCCY	2 cut(s)	1232, 1564
Psp6I	CCWGG	3 cut(s)	192, 1370, 1894
PspEI	GGTNACC	1 cut(s)	521
PspGI	CCWGG	3 cut(s)	192, 1370, 1894
PspN4I	GGNNCC	1 cut(s)	1039
PspPI	GGNCC	5 cut(s)	306, 1232, 1564, 1693, 1739
PspPPI	RGGWCCY	2 cut(s)	1232, 1564
PstNI	CAGNNNCTG	2 cut(s)	1606, 1978
PsuI	RGATCY	2 cut(s)	692, 1591
PteI	GCGCGC	1 cut(s)	1838
PvuII	CAGCTG	2 cut(s)	1256, 1975
RsaI	GTAC	3 cut(s)	822, 900, 1132
RsaNI	GTAC	3 cut(s)	821, 899, 1131
RseI	CAYNNNNRTG	4 cut(s)	1386, 1398, 1859, 1888
SacI	GAGCTC	1 cut(s)	353
SalI	GTCGAC	1 cut(s)	1848
SaqAI	TTAA	8 cut(s)	254, 413, 597, 672, 1001, 1086, 1956, 2000
SatI	GCNGC	7 cut(s)	1146, 1155, 1811, 1829, 1881, 1973, 1976
Sau3AI	GATC	7 cut(s)	127, 145, 692, 1078, 1222, 1591, 2017
Sau96I	GGNCC	5 cut(s)	306, 1232, 1564, 1693, 1739
SchI	GAGTC	3 cut(s)	399, 1453, 1845
ScrFI	CCNGG	5 cut(s)	98, 115, 194, 1372, 1896
SduI	GDGCHC	2 cut(s)	353, 1895
SfaNI	GCATC	6 cut(s)	577, 751, 902, 1783, 1867, 1892
SfcI	CTRYAG	3 cut(s)	771, 1365, 1479
SinI	GGWCC	2 cut(s)	1232, 1564
SmiI	ATTTAAAT	1 cut(s)	255
SmiMI	CAYNNNNRTG	4 cut(s)	1386, 1398, 1859, 1888
SmlI	CTYRAG	3 cut(s)	554, 920, 1931
SmoI	CTYRAG	3 cut(s)	554, 920, 1931
SpeI	ACTAGT	1 cut(s)	424
SseBI	AGGCCT	1 cut(s)	410
SsiI	CCGC	5 cut(s)	111, 170, 190, 687, 1386
SspMI	CTAG	9 cut(s)	425, 684, 1064, 1209, 1425, 1469, 1475, 1664, 1718
SstI	GAGCTC	1 cut(s)	353
StuI	AGGCCT	1 cut(s)	410
StyD4I	CCNGG	5 cut(s)	96, 113, 192, 1370, 1894
StyI	CCWWGG	5 cut(s)	313, 329, 655, 1063, 1431
SwaI	ATTTAAAT	1 cut(s)	255
TaaI	ACNGT	4 cut(s)	1057, 1354, 1532, 1996
TaiI	ACGT	2 cut(s)	36, 1033
TaqI	TCGA	5 cut(s)	126, 144, 207, 1849, 1909
TatI	WGTACW	1 cut(s)	898
TfiI	GAWTC	7 cut(s)	107, 266, 419, 438, 449, 995, 2029
Tru1I	TTAA	8 cut(s)	254, 413, 597, 672, 1001, 1086, 1956, 2000
Tru9I	TTAA	8 cut(s)	254, 413, 597, 672, 1001, 1086, 1956, 2000
TscAI	CASTG	4 cut(s)	763, 1388, 1893, 1999
TseFI	GTSAC	5 cut(s)	521, 1192, 1487, 1620, 2044
TseI	GCWGC	7 cut(s)	1145, 1154, 1810, 1828, 1880, 1972, 1975
Tsp45I	GTSAC	5 cut(s)	521, 1192, 1487, 1620, 2044
TspGWI	ACGGA	3 cut(s)	214, 450, 636
TspRI	CASTG	4 cut(s)	763, 1388, 1893, 1999
VpaK11BI	GGWCC	2 cut(s)	1232, 1564
XapI	RAATTY	4 cut(s)	256, 368, 668, 978
XbaI	TCTAGA	1 cut(s)	1663
XcmI	CCANNNNNNNNNTGG	1 cut(s)	922
XmaJI	CCTAGG	1 cut(s)	1063
XmiI	GTMKAC	2 cut(s)	228, 1849
XmnI	GAANNNNTTC	1 cut(s)	471
XspI	CTAG	9 cut(s)	425, 684, 1064, 1209, 1425, 1469, 1475, 1664, 1718
ZraI	GACGTC	1 cut(s)	34

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.