Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0008285

Physical Location & Seq

Genomic Coordinates Forward (+)

49616975 .. 49626341

Gene Structure 9367 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000025980

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3735 bp

ATGTGTGGGATATTTGCGTACCTGAATTACAACGTGAACCGAGAGAGGAGTTATATTCTTCAGGTGCTATTCAATGGCCTCCGGCGATTGGAGTACCGGGGCTACGATTCCGCCGGAATTTCGATCGATCATTCCTCCTCTGATCCCAAAACCTCCTCTCTCCCTCCCTCTCTCGTTTTTCGCCAGGAGGGTAACATCGAGTCGCTCGTCAAATCCGTCTACCAAGAGGTGGCTGAAACAGACCTAAATTTGAAGCAGTGTTTCTCTCACCATGCTGGAATAGCACATACCCGGTGGGCCACTCATGGAGAGCCCGCTCCAAGGAACAGCCACCCTCAGAGCTCTGATCCTGGAAATGAATTTTTGGTCGTTCACAATGGAGTTATTACTAATTACGAGGCCTTAAAGGGCACTCTAGTTCGGCATGGATTTACCTTTCAATCTGAAACAGACACAGAAGTTATTCCAAAGCTTGCCAAATATGTCTTTGATAAAGCTAAAGAAGGAGAAGGTGACCAGACTATCACATTTAGTCAAGTAGTGCATGAGGTTATGAGGCATCTTGAAGGAGCCTATGCGCTGATTTTTAAAAGCCGGCATTATCCAAATGAGTTGATTGCTTGTAAACGCGGCAGTCCTTTGCTCCTTGGTGTGAAAGAACTTAACGAAAATGCTAGCAATGGTTCTGCATTTCATGATGATAATTTCCTTTCAAAAAGTGGACATCCTAAAGAACTCTTCTTGTCCAGTGATGCCAATGCCGTAGTTGAACACACCAAAAAGGTTTTGGTGATTGAGGATGGTGAAGTGGTGCATCTTAAGGATGGACACGTGTCAATCCTAAAGTTTGATAAAGGAAAGCATGGTGGCCTTTCAAAAGTTGCATCAGTACAACGAGCATTGTCCATTCTTGAGATGGAGGTTGAACAAATAAATAAAGGAAATTTTGAACACTACATGCAGAAAGAAATTCATGAGCAGCCTGAATCTCTAACTACCACAATGAGGGGTAGGCTTTTACGTGGTGGTTCCTGCAAAGCCAAGACTGTTCTCCTTGGCGGACTGAAGGACCACCTTAAAACAATTCGGCGAAGCAGGCGAATTGTTTTCATTGGCTGTGGTACAAGCTACAATGCTGCTTTAGCTGCGAGACCTATCTTGGAAGAACTTTCTGGTATCCCTGTTACAATGGAAATTGCTAGTGATCTGTTGGATCGGCAAGGACCAATTTACAGAGAAGATACAGCTGTGTTTGTCAGTCAATCTGGTGAAACAGCAGATACGTTGAGTGCATTGGAATATGCTTTAGAAAATGGAGCATTATGCGTTGGCATAACAAATACCGTTGGTAGTGCAATAGCCCGTAACACACACTGTGGTGTTCATATAAATGCTGGTGCTGAGATTGGAGTGGCAAGCACCAAGGCTTACACAAGTCAAATAGTGGTGATGGCTATGCTAGCCCTAGCAATCGGAGGTGACACAATTTCCAATCAAGCAAGAAGAGAGGCTATAATTGACGGTTTATTTGACCTGCCAAACAAAGTCAGAGAGGTCCTGAAGCTTGACCAGGTAATGAAGGATCTTGCAAAACAATTGATAGCAGAGCAATCACTTCTTGTTTTTGGGAGAGGATACAACTATGCATCGGCTTTGGAGGGTGCTTTGAAGGTAAAGGAAGTAGCACTCATGCATAGCGAAGGAATACTTGCCGGTGAAATGAAACATGGTCCTTTGGCTTTAGTTGATGAAAATCTCCCAACTTTTGTGATTGCTACCCGTGATGCTTGCTTCAGTAAGCAGCAATCAGTTATTCAGCAGCTTCATGCCCGCAAAGGTCGTCTGATTGTAATGTGTTCGAAAGGAGATGCTGCATCTGTTTGCCCTGCGGAATGGGAGTCTTGTCGAGTGATTGAAGTTCCTCAGCTTGAGGATTGTCTTCAACCTGTAGTCAATATAGTTCCCTTGCAGCTGCTAGCATACCATCTCACAGTTTTAAGAGGGTTTAATGTGGATCAGCCTCGGAATCTTGCAAAGAATACTAGTCTAGTGAAAGAGGAGGATATCAAAACTAAGAAGAAGAAAAAGAATAGCAATGAAACAGAAGAGGAGGATAGCAAAACTAAGAAGAAGAAGAAGAAGAAAAAGGTCTACTTGAAGGATGTAGTGGCTAATCATTTATTAGAAGATGGTGCCGAACTTCCGGAGGATGATGGTAAGCAGTTCCTCAGGAGTTATAGTGAAGAGCAAGAGCAGATAAGGAGGGAGTTTTTGGAAGCAGCTGCCGAGGAGGAGGAGGATGATGAGGGGGAGATATTGAGAGTCAAAGGCAGTAATAGTGCTGTGGGGGATGAAGCTGATGAGGAGTTTGATAAGAAATTGGATGAGTATTTCGGTCAAGACTTGGATGACAATGCTAGGTTTCTCAGGGACTTCTTTAAGAATAAGATGTGGTTGGACAAGGGGAATCAGGGAAGAGGTGCTACTGAGGTTAGGGATGAGGATTTGGAGATGGTGTCTGAGGACGAGATAGAGATTGAGAGGCAGGAGGAGTACGAGTATAGGTTCCAGGAGAATGCAGGGGATCAGGTTTTGGGGCATGCCAGGGAGGTCGAGGGGTCGGTGAGGAAGAAGGTCAAGGCAAGGAAGGAGCAGAGGAAGAGCAAGGAGGAGAGGATGGAGATTGCAAGGTTGGAGAGGGAGGAGGAGTTGAGGCATTTGAAGAATTTGAAGAAGAAGGAGAATGATGACAGGGTCAAGAAAATAATGAAGATTGCAGGGATCAAAGAGGGCGAGGAGTCCTTGTTTGATTCCAAGGAGTTGGAGAACGAATTTGATCCCCAGGAGTATGATACGATGATGAAGAAAGCTTTTGATGATGAGTATTATGGAAATGATGACTCAGACTTTTGTAGCGATATGGATGAGGACGGTGGTGAGATTGAGAAACCGGATTTTGATAAGGAGGACGAGTTACTTGGACTTCCCAGTGGTTGGGATGGTGCTAGGTCTGGTGATGGTTTCTTAGCTGCGAGGGAGAAGATTTTAAAGCTAAAAAAGGAGACTGGCAGTGATAATGAAGAAGAGGAAGAAGACCAGGAGGAAAAGAAAGAAGAAGATAGTGAAGAAGATGAAGGAGAAGGAAAAGGAGAAGAAGAGAAACTGAGCGAGGAAGGTAAACAAGAGAAAAAGCGTAAATTAGCTATTTTGGAGAGAGCTAAAAAGGAGATGTTGGATGAATACTATAAATTAGACTATGAGGATACAATTGGAGACTTGAAGACTAGATTTAGGTATGCTAAAATAAAGCCTAATAGATTTGGGTTGACTACTGCTGAGGTATTAGTGATGGATGAAAAGGAGTTAAATCAATATGTATCTTTGAAAAAGCTTGCTCCATATGCGGAGAAGGAGTGGAAGGTACCCAATAGTAAGAGAAATGAGATAAAGAAGAGGGCCAGGGAGATTTTCAGACATGGAAAAATGGATCACAAAAAAAGTGCTAAGAAGTTGAAAATTAAGGATGATGTGAAGGATTCAATTTCATCAACACCAGCTAAGGAACGTGGGAAGAAACAAGTACAGGACTCAAATGAACAAAGGAAAGCAGTGATCCGAGCAATAAACAGGTTTGATGTTTTCATAAATGGAGTGGGAGGACTACAATTTTGCAGTGGTGAGAAGCGTGTTGGGGATTTTGTCCAACAAAACAAAGGCAAACAGGGTAGAAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004360 GO:0006002 GO:0006040 GO:0006047 GO:0006139 GO:0006464 GO:0006486 GO:0006487 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008483 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009225 GO:0009987 GO:0016740 GO:0016769 GO:0019538 GO:0019637 GO:0034641 GO:0034645 GO:0036211 GO:0043170 GO:0043412 GO:0043413 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0046483 GO:0055086 GO:0070085 GO:0070548 GO:0071704 GO:1901135 GO:1901137 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00250 ko00520 ko01100 ko01130 ko04931 map00250 map00520 map01100 map01130 map04931

Pfam Domains

Protein Families

GATase_6 SIS

Protein Analysis

1245

Amino Acids

140.43

Weight (kDa)

5.57

Isoelectric Point (pI)

44.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GATase_6	PF13522	89 - 205	1.1e-13	Glutamine amidotransferase domain
GATase_7	PF13537	108 - 201	3e-09	Glutamine amidotransferase domain
SIS	PF01380	362 - 490	5e-34	SIS domain
SIS	PF01380	533 - 666	2.2e-18	SIS domain
Kri1	PF05178	891 - 991	1.2e-18	KRI1-like family
Kri1_C	PF12936	1074 - 1156	1.9e-29	KRI1-like family C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0008285)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G24090
fragaria_vesca	FvH4_6g00360 FvH4_6g00360
malus_domestica	MD04G1173900.v1.1 MD04G1246300.v1.1 MD12G1263800.v1.1
prunus_persica	Prupe.6G362600_v2.0.a1
pyrus_communis	pycom04g21700 pycom12g24000
rosa_chinensis	RchiOBHm_Chr3g0447661 RchiOBHm_Chr4g0412421
rosa_laevigata	RLG00000025980
rosa_multiflora	Rmu_ssc0000391.1_g000002
rosa_roxburghii	Rroxscaffold_6G00425870
rosa_rugosa	Rorug02G0604100 Rorug04G0077300 Rorug07G0328400
rosa_samantha	Rh3AG004100 Rh3BG003700 Rh3CG004000 Rh3DG004000 Rh4CG183700
rosa_wichuraiana	Rw3G000310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1542
Acc65I	GGTACC	1 cut(s)	3421
AccB1I	GGYRCC	2 cut(s)	2192, 3421
AccB7I	CCANNNNNTGG	2 cut(s)	229, 2994
AccBSI	CCGCTC	1 cut(s)	317
AccI	GTMKAC	2 cut(s)	219, 2151
AccII	CGCG	1 cut(s)	630
AccIII	TCCGGA	1 cut(s)	2203
AciI	CCGC	7 cut(s)	111, 315, 630, 1059, 1831, 1889, 3404
AcsI	RAATTY	7 cut(s)	117, 247, 359, 943, 969, 2725, 2831
AcuI	CTGAAG	4 cut(s)	44, 1085, 1580, 1777
AcvI	CACGTG	1 cut(s)	832
AdeI	CACNNNGTG	1 cut(s)	1376
AfaI	GTAC	7 cut(s)	20, 95, 891, 1123, 2555, 3423, 3582
AfiI	CCNNNNNNNGG	5 cut(s)	88, 229, 321, 1005, 2994
AflII	CTTAAG	1 cut(s)	818
AflIII	ACRYGT	2 cut(s)	829, 831
AhlI	ACTAGT	1 cut(s)	2042
AjnI	CCWGG	8 cut(s)	183, 349, 1569, 2568, 2603, 2841, 3096, 3458
AleI	CACNNNNGTG	1 cut(s)	1377
Alw21I	GWGCWC	1 cut(s)	344
Alw26I	GTCTC	3 cut(s)	1144, 3056, 3267
Aor13HI	TCCGGA	1 cut(s)	2203
AoxI	GGCC	5 cut(s)	76, 297, 399, 868, 3456
ApoI	RAATTY	7 cut(s)	117, 247, 359, 943, 969, 2725, 2831
ArsI	GACNNNNNNTTYG	4 cut(s)	1511, 1543, 3651, 3683
Asp700I	GAANNNNTTC	1 cut(s)	462
Asp718I	GGTACC	1 cut(s)	3421
AspLEI	GCGC	1 cut(s)	580
AspS9I	GGNCC	6 cut(s)	297, 1069, 1223, 1555, 1730, 3456
AsuC2I	CCSGG	2 cut(s)	98, 292
AsuII	TTCGAA	1 cut(s)	1859
AsuNHI	GCTAGC	3 cut(s)	674, 1459, 1975
AvaII	GGWCC	4 cut(s)	1069, 1223, 1555, 1730
AxyI	CCTNAGG	1 cut(s)	2228
BaeGI	GKGCMC	1 cut(s)	413
BaeI	ACNNNNGTAYC	2 cut(s)	1233, 1266
BanI	GGYRCC	2 cut(s)	2192, 3421
BanII	GRGCYC	2 cut(s)	315, 344
BarI	GAAGNNNNNNTAC	2 cut(s)	2467, 2499
BbrPI	CACGTG	1 cut(s)	832
BbsI	GAAGAC	3 cut(s)	1931, 3099, 3287
Bbv12I	GWGCWC	1 cut(s)	344
BbvCI	CCTCAGC	2 cut(s)	1923, 3336
BceAI	ACGGC	1 cut(s)	746
BciT130I	CCWGG	8 cut(s)	185, 351, 1571, 2570, 2605, 2843, 3098, 3460
BciVI	GTATCC	3 cut(s)	1187, 1628, 3256
BcnI	CCSGG	2 cut(s)	98, 292
BcoDI	GTCTC	3 cut(s)	1144, 3056, 3267
BcuI	ACTAGT	1 cut(s)	2042
BfmI	CTRYAG	1 cut(s)	1947
BfrI	CTTAAG	1 cut(s)	818
BfuAI	ACCTGC	1 cut(s)	1542
BfuI	GTATCC	3 cut(s)	1187, 1628, 3256
Bme18I	GGWCC	4 cut(s)	1069, 1223, 1555, 1730
BmgT120I	GGNCC	6 cut(s)	297, 1069, 1223, 1555, 1730, 3456
BmiI	GGNNCC	5 cut(s)	571, 1030, 2194, 2567, 3423
BmrI	ACTGGG	1 cut(s)	2982
BmsI	GCATC	8 cut(s)	568, 742, 823, 893, 1655, 1774, 1858, 1883
BmtI	GCTAGC	3 cut(s)	678, 1463, 1979
BmuI	ACTGGG	1 cut(s)	2982
BoxI	GACNNNNGTC	1 cut(s)	832
BpiI	GAAGAC	3 cut(s)	1931, 3099, 3287
Bpu10I	CCTNAGC	3 cut(s)	1923, 3336, 3558
Bpu14I	TTCGAA	1 cut(s)	1859
BpuEI	CTTGAG	2 cut(s)	932, 1949
BpuMI	CCSGG	2 cut(s)	98, 292
Bsa29I	ATCGAT	1 cut(s)	126
BsaAI	YACGTR	2 cut(s)	832, 1022
BsaBI	GATNNNNATC	1 cut(s)	1752
BsaI	GGTCTC	1 cut(s)	1144
BsaWI	WCCGGW	2 cut(s)	2203, 2950
Bsc4I	CCNNNNNNNGG	5 cut(s)	88, 229, 321, 1005, 2994
Bse118I	RCCGGY	2 cut(s)	594, 1712
Bse1I	ACTGG	3 cut(s)	747, 2988, 3070
Bse21I	CCTNAGG	1 cut(s)	2228
Bse3DI	GCAATG	2 cut(s)	685, 2101
Bse8I	GATNNNNATC	1 cut(s)	1752
BseAI	TCCGGA	1 cut(s)	2203
BseBI	CCWGG	8 cut(s)	185, 351, 1571, 2570, 2605, 2843, 3098, 3460
BseCI	ATCGAT	1 cut(s)	126
BseJI	GATNNNNATC	1 cut(s)	1752
BseLI	CCNNNNNNNGG	5 cut(s)	88, 229, 321, 1005, 2994
BseMI	GCAATG	2 cut(s)	685, 2101
BseNI	ACTGG	3 cut(s)	747, 2988, 3070
BseSI	GKGCMC	1 cut(s)	413
Bsh1236I	CGCG	1 cut(s)	630
Bsh1285I	CGRYCG	1 cut(s)	126
BshFI	GGCC	5 cut(s)	78, 299, 401, 870, 3458
BshNI	GGYRCC	2 cut(s)	2192, 3421
BshVI	ATCGAT	1 cut(s)	126
BsiEI	CGRYCG	1 cut(s)	126
BsiHKAI	GWGCWC	1 cut(s)	344
BsiSI	CCGG	8 cut(s)	82, 97, 114, 292, 595, 1713, 2204, 2951
BslFI	GGGAC	1 cut(s)	2444
BslI	CCNNNNNNNGG	5 cut(s)	88, 229, 321, 1005, 2994
BsmAI	GTCTC	3 cut(s)	1144, 3056, 3267
BsmFI	GGGAC	1 cut(s)	2444
BsmI	GAATGC	1 cut(s)	2581
BsnI	GGCC	5 cut(s)	78, 299, 401, 870, 3458
Bso31I	GGTCTC	1 cut(s)	1144
Bsp119I	TTCGAA	1 cut(s)	1859
Bsp1286I	GDGCHC	3 cut(s)	315, 344, 413
Bsp13I	TCCGGA	1 cut(s)	2203
BspACI	CCGC	7 cut(s)	111, 315, 630, 1059, 1831, 1889, 3404
BspANI	GGCC	5 cut(s)	78, 299, 401, 870, 3458
BspDI	ATCGAT	1 cut(s)	126
BspEI	TCCGGA	1 cut(s)	2203
BspFNI	CGCG	1 cut(s)	630
BspHI	TCATGA	2 cut(s)	694, 973
BspLI	GGNNCC	5 cut(s)	571, 1030, 2194, 2567, 3423
BspMI	ACCTGC	1 cut(s)	1542
BspOI	GCTAGC	3 cut(s)	678, 1463, 1979
BspQI	GCTCTTC	2 cut(s)	2238, 2654
BspT104I	TTCGAA	1 cut(s)	1859
BspT107I	GGYRCC	2 cut(s)	2192, 3421
BspTI	CTTAAG	1 cut(s)	818
BspTNI	GGTCTC	1 cut(s)	1144
BsrBI	CCGCTC	1 cut(s)	317
BsrDI	GCAATG	2 cut(s)	685, 2101
BsrFI	RCCGGY	2 cut(s)	594, 1712
BsrI	ACTGG	3 cut(s)	747, 2988, 3070
BssAI	RCCGGY	2 cut(s)	594, 1712
BssT1I	CCWWGG	5 cut(s)	320, 646, 1054, 1422, 2814
Bst2UI	CCWGG	8 cut(s)	185, 351, 1571, 2570, 2605, 2843, 3098, 3460
Bst4CI	ACNGT	6 cut(s)	1048, 1345, 1376, 1523, 1993, 2933
Bst6I	CTCTTC	9 cut(s)	743, 1498, 2100, 2238, 2470, 2654, 3078, 3150, 3446
BstAFI	CTTAAG	1 cut(s)	818
BstBAI	YACGTR	2 cut(s)	832, 1022
BstBI	TTCGAA	1 cut(s)	1859
BstEII	GGTNACC	1 cut(s)	512
BstFNI	CGCG	1 cut(s)	630
BstHHI	GCGC	1 cut(s)	580
BstMAI	GTCTC	3 cut(s)	1144, 3056, 3267
BstMCI	CGRYCG	1 cut(s)	126
BstMWI	GCNNNNNNNGC	6 cut(s)	281, 1096, 1142, 1145, 1460, 3401
BstNI	CCWGG	8 cut(s)	185, 351, 1571, 2570, 2605, 2843, 3098, 3460
BstNSI	RCATGY	2 cut(s)	961, 2603
BstPAI	GACNNNNGTC	1 cut(s)	832
BstPI	GGTNACC	1 cut(s)	512
BstSFI	CTRYAG	1 cut(s)	1947
BstSLI	GKGCMC	1 cut(s)	413
BstUI	CGCG	1 cut(s)	630
BstV2I	GAAGAC	3 cut(s)	1931, 3099, 3287
BstX2I	RGATCY	1 cut(s)	1582
BstXI	CCANNNNNNTGG	1 cut(s)	2821
BstYI	RGATCY	1 cut(s)	1582
Bsu15I	ATCGAT	1 cut(s)	126
Bsu36I	CCTNAGG	1 cut(s)	2228
BsuI	GTATCC	3 cut(s)	1187, 1628, 3256
BsuRI	GGCC	5 cut(s)	78, 299, 401, 870, 3458
BsuTUI	ATCGAT	1 cut(s)	126
BtsI	GCAGTG	4 cut(s)	263, 3076, 3615, 3679
BtsIMutI	CAGTG	7 cut(s)	263, 754, 1372, 2995, 3076, 3615, 3679
BveI	ACCTGC	1 cut(s)	1542
CciI	TCATGA	2 cut(s)	694, 973
CfoI	GCGC	1 cut(s)	580
Cfr10I	RCCGGY	2 cut(s)	594, 1712
Cfr13I	GGNCC	6 cut(s)	297, 1069, 1223, 1555, 1730, 3456
ClaI	ATCGAT	1 cut(s)	126
CsiI	ACCWGGT	1 cut(s)	1569
Csp6I	GTAC	7 cut(s)	19, 94, 890, 1122, 2554, 3422, 3581
CviQI	GTAC	7 cut(s)	19, 94, 890, 1122, 2554, 3422, 3581
DraI	TTTAAA	2 cut(s)	589, 3048
DraIII	CACNNNGTG	1 cut(s)	1376
Eam1104I	CTCTTC	9 cut(s)	743, 1498, 2100, 2238, 2470, 2654, 3078, 3150, 3446
EarI	CTCTTC	9 cut(s)	743, 1498, 2100, 2238, 2470, 2654, 3078, 3150, 3446
EciI	GGCGGA	2 cut(s)	100, 1074
Ecl136II	GAGCTC	1 cut(s)	342
Eco130I	CCWWGG	5 cut(s)	320, 646, 1054, 1422, 2814
Eco147I	AGGCCT	1 cut(s)	401
Eco24I	GRGCYC	2 cut(s)	315, 344
Eco31I	GGTCTC	1 cut(s)	1144
Eco32I	GATATC	1 cut(s)	2065
Eco47I	GGWCC	4 cut(s)	1069, 1223, 1555, 1730
Eco53kI	GAGCTC	1 cut(s)	342
Eco57I	CTGAAG	4 cut(s)	44, 1085, 1580, 1777
Eco72I	CACGTG	1 cut(s)	832
Eco81I	CCTNAGG	1 cut(s)	2228
Eco91I	GGTNACC	1 cut(s)	512
EcoICRI	GAGCTC	1 cut(s)	342
EcoO109I	RGGNCCY	1 cut(s)	1555
EcoO65I	GGTNACC	1 cut(s)	512
EcoRII	CCWGG	8 cut(s)	183, 349, 1569, 2568, 2603, 2841, 3096, 3458
EcoRV	GATATC	1 cut(s)	2065
EcoT14I	CCWWGG	5 cut(s)	320, 646, 1054, 1422, 2814
EcoT22I	ATGCAT	2 cut(s)	1648, 1695
EcoT38I	GRGCYC	2 cut(s)	315, 344
ErhI	CCWWGG	5 cut(s)	320, 646, 1054, 1422, 2814
FalI	AAGNNNNNCTT	4 cut(s)	1692, 1724, 2138, 2170
FaqI	GGGAC	1 cut(s)	2444
FauI	CCCGC	2 cut(s)	322, 1838
FauNDI	CATATG	1 cut(s)	3400
FblI	GTMKAC	2 cut(s)	219, 2151
FriOI	GRGCYC	2 cut(s)	315, 344
GlaI	GCGC	1 cut(s)	579
HaeIII	GGCC	5 cut(s)	78, 299, 401, 870, 3458
HapII	CCGG	8 cut(s)	82, 97, 114, 292, 595, 1713, 2204, 2951
HhaI	GCGC	1 cut(s)	580
Hin6I	GCGC	1 cut(s)	578
HinP1I	GCGC	1 cut(s)	578
HincII	GTYRAC	1 cut(s)	3327
HindII	GTYRAC	1 cut(s)	3327
HindIII	AAGCTT	4 cut(s)	470, 1562, 2868, 3389
HpaII	CCGG	8 cut(s)	82, 97, 114, 292, 595, 1713, 2204, 2951
Hpy166II	GTNNAC	8 cut(s)	37, 220, 373, 626, 722, 2152, 3179, 3327
Hpy188III	TCNNGA	9 cut(s)	563, 695, 911, 974, 1558, 2204, 2230, 2399, 2758
Hpy8I	GTNNAC	8 cut(s)	37, 220, 373, 626, 722, 2152, 3179, 3327
HpyCH4III	ACNGT	6 cut(s)	1048, 1345, 1376, 1523, 1993, 2933
HpyCH4IV	ACGT	5 cut(s)	33, 831, 1021, 1283, 3565
HpyF10VI	GCNNNNNNNGC	6 cut(s)	281, 1096, 1142, 1145, 1460, 3401
HpySE526I	ACGT	5 cut(s)	33, 831, 1021, 1283, 3565
HspAI	GCGC	1 cut(s)	578
Kpn2I	TCCGGA	1 cut(s)	2203
KpnI	GGTACC	1 cut(s)	3425
KroI	GCCGGC	1 cut(s)	594
KroNI	GCCGGC	1 cut(s)	596
LguI	GCTCTTC	2 cut(s)	2238, 2654
LmnI	GCTCC	6 cut(s)	322, 569, 648, 1316, 2650, 3400
LweI	GCATC	8 cut(s)	568, 742, 823, 893, 1655, 1774, 1858, 1883
MabI	ACCWGGT	1 cut(s)	1569
MaeII	ACGT	5 cut(s)	33, 831, 1021, 1283, 3565
MaeIII	GTNAC	6 cut(s)	191, 512, 1183, 1364, 1478, 2973
MbiI	CCGCTC	1 cut(s)	317
MfeI	CAATTG	2 cut(s)	1595, 3267
MflI	RGATCY	1 cut(s)	1582
MhlI	GDGCHC	3 cut(s)	315, 344, 413
MlyI	GAGTC	6 cut(s)	209, 1907, 2331, 2807, 2894, 3581
MmeI	TCCRAC	6 cut(s)	1191, 2436, 2673, 2802, 3213, 3727
Mph1103I	ATGCAT	2 cut(s)	1648, 1695
MroI	TCCGGA	1 cut(s)	2203
MroNI	GCCGGC	1 cut(s)	594
MroXI	GAANNNNTTC	1 cut(s)	462
MslI	CAYNNNNRTG	2 cut(s)	1377, 1389
MspA1I	CMGCKG	3 cut(s)	1247, 1972, 2282
MspCI	CTTAAG	1 cut(s)	818
MspI	CCGG	8 cut(s)	82, 97, 114, 292, 595, 1713, 2204, 2951
MunI	CAATTG	2 cut(s)	1595, 3267
Mva1269I	GAATGC	1 cut(s)	2581
MvaI	CCWGG	8 cut(s)	185, 351, 1571, 2570, 2605, 2843, 3098, 3460
MvnI	CGCG	1 cut(s)	630
MwoI	GCNNNNNNNGC	6 cut(s)	281, 1096, 1142, 1145, 1460, 3401
NaeI	GCCGGC	1 cut(s)	596
NciI	CCSGG	2 cut(s)	98, 292
NdeI	CATATG	1 cut(s)	3400
NgoMIV	GCCGGC	1 cut(s)	594
NheI	GCTAGC	3 cut(s)	674, 1459, 1975
NlaIV	GGNNCC	5 cut(s)	571, 1030, 2194, 2567, 3423
NmeAIII	GCCGAG	1 cut(s)	2311
NmuCI	GTSAC	2 cut(s)	512, 1478
NsiI	ATGCAT	2 cut(s)	1648, 1695
NspI	RCATGY	2 cut(s)	961, 2603
NspV	TTCGAA	1 cut(s)	1859
OliI	CACNNNNGTG	1 cut(s)	1377
PaeI	GCATGC	1 cut(s)	2603
PagI	TCATGA	2 cut(s)	694, 973
PceI	AGGCCT	1 cut(s)	401
PciSI	GCTCTTC	2 cut(s)	2238, 2654
PcsI	WCGNNNNNNNCGW	2 cut(s)	204, 213
PctI	GAATGC	1 cut(s)	2581
PdiI	GCCGGC	1 cut(s)	596
PdmI	GAANNNNTTC	1 cut(s)	462
PfeI	GAWTC	6 cut(s)	107, 986, 2026, 2467, 2810, 3536
PflFI	GACNNNGTC	1 cut(s)	2753
PflMI	CCANNNNNTGG	2 cut(s)	229, 2994
PfoI	TCCNGGA	2 cut(s)	349, 2568
Ple19I	CGATCG	1 cut(s)	126
PleI	GAGTC	6 cut(s)	208, 1906, 2330, 2806, 2894, 3581
PmaCI	CACGTG	1 cut(s)	832
PmlI	CACGTG	1 cut(s)	832
PpsI	GAGTC	6 cut(s)	208, 1906, 2330, 2806, 2894, 3581
Ppu21I	YACGTR	2 cut(s)	832, 1022
PpuMI	RGGWCCY	1 cut(s)	1555
PshAI	GACNNNNGTC	1 cut(s)	832
Psp124BI	GAGCTC	1 cut(s)	344
Psp5II	RGGWCCY	1 cut(s)	1555
Psp6I	CCWGG	8 cut(s)	183, 349, 1569, 2568, 2603, 2841, 3096, 3458
PspCI	CACGTG	1 cut(s)	832
PspEI	GGTNACC	1 cut(s)	512
PspGI	CCWGG	8 cut(s)	183, 349, 1569, 2568, 2603, 2841, 3096, 3458
PspN4I	GGNNCC	5 cut(s)	571, 1030, 2194, 2567, 3423
PspPI	GGNCC	6 cut(s)	297, 1069, 1223, 1555, 1730, 3456
PspPPI	RGGWCCY	1 cut(s)	1555
PsuI	RGATCY	1 cut(s)	1582
PsyI	GACNNNGTC	1 cut(s)	2753
PvuI	CGATCG	1 cut(s)	126
PvuII	CAGCTG	3 cut(s)	1247, 1972, 2282
RsaI	GTAC	7 cut(s)	20, 95, 891, 1123, 2555, 3423, 3582
RsaNI	GTAC	7 cut(s)	19, 94, 890, 1122, 2554, 3422, 3581
RseI	CAYNNNNRTG	2 cut(s)	1377, 1389
SacI	GAGCTC	1 cut(s)	344
SapI	GCTCTTC	2 cut(s)	2238, 2654
Sau96I	GGNCC	6 cut(s)	297, 1069, 1223, 1555, 1730, 3456
SchI	GAGTC	6 cut(s)	209, 1907, 2331, 2807, 2894, 3581
SduI	GDGCHC	3 cut(s)	315, 344, 413
SexAI	ACCWGGT	1 cut(s)	1569
SfaNI	GCATC	8 cut(s)	568, 742, 823, 893, 1655, 1774, 1858, 1883
SfcI	CTRYAG	1 cut(s)	1947
SfuI	TTCGAA	1 cut(s)	1859
SinI	GGWCC	4 cut(s)	1069, 1223, 1555, 1730
SmiMI	CAYNNNNRTG	2 cut(s)	1377, 1389
SmlI	CTYRAG	3 cut(s)	818, 911, 1928
SmoI	CTYRAG	3 cut(s)	818, 911, 1928
SpeI	ACTAGT	1 cut(s)	2042
SphI	GCATGC	1 cut(s)	2603
SseBI	AGGCCT	1 cut(s)	401
SsiI	CCGC	7 cut(s)	111, 315, 630, 1059, 1831, 1889, 3404
SstI	GAGCTC	1 cut(s)	344
StuI	AGGCCT	1 cut(s)	401
StyI	CCWWGG	5 cut(s)	320, 646, 1054, 1422, 2814
TaaI	ACNGT	6 cut(s)	1048, 1345, 1376, 1523, 1993, 2933
TaiI	ACGT	5 cut(s)	36, 834, 1024, 1286, 3568
TaqI	TCGA	6 cut(s)	122, 126, 198, 1859, 1906, 2613
TaqII	GACCGA	1 cut(s)	2384
TatI	WGTACW	2 cut(s)	889, 3580
TauI	GCSGC	1 cut(s)	633
TfiI	GAWTC	6 cut(s)	107, 986, 2026, 2467, 2810, 3536
TscAI	CASTG	7 cut(s)	263, 754, 1379, 2995, 3076, 3615, 3679
TseFI	GTSAC	2 cut(s)	512, 1478
Tsp45I	GTSAC	2 cut(s)	512, 1478
TspGWI	ACGGA	1 cut(s)	205
TspRI	CASTG	7 cut(s)	263, 754, 1379, 2995, 3076, 3615, 3679
Tth111I	GACNNNGTC	1 cut(s)	2753
Van91I	CCANNNNNTGG	2 cut(s)	229, 2994
Vha464I	CTTAAG	1 cut(s)	818
VpaK11BI	GGWCC	4 cut(s)	1069, 1223, 1555, 1730
XapI	RAATTY	7 cut(s)	117, 247, 359, 943, 969, 2725, 2831
XceI	RCATGY	2 cut(s)	961, 2603
XcmI	CCANNNNNNNNNTGG	2 cut(s)	784, 913
XmiI	GTMKAC	2 cut(s)	219, 2151
XmnI	GAANNNNTTC	1 cut(s)	462
Zsp2I	ATGCAT	2 cut(s)	1648, 1695

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.