MD04G1246500.v1.1

protein KRI1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0009859
Physical Location & Seq
Forward (+)
32143731 .. 32145266
1536 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1246500.v1.1.491

Sequence Viewer

Length: 396 bp
ATGGATGAAAAGGAGTTGAATCAATATGTGTCTTTAAAGAAGCTTGCTCCTTACACAGAGAAAGAGTGGAAGGTACCCAACAACAAGAGAATGGAGATAAAGCAGAAAGCTAAAGAGATTTTCAGACAAGGAAATGTGGGTAATAAAAAGAATCGCAAGAAGCTGAGAATAAGTGATGCAGCTAAGGATTCGAGCTTATCAAAGGGTGCCACAGGGCAATCATCAATGAGTGCCCGGGAACATGGGAAAGAACTAGTAGGGGACTCAAATGGTGGTAAAACAGAATCGAGGAGTGCTAGAAGAAGGCGTCACCAAGCTGCGAGAAAATTACCAGCTTCTAGGCTCATGGCATATGGGGTGGTACCTGTAAAATCTAAGAAAAAAGGAAAGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kri1 Kri1_C

Protein Analysis

132

Amino Acids

14.78

Weight (kDa)

10.58

Isoelectric Point (pI)

54.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kri1_C PF12936 1 - 40 5.6e-09 KRI1-like family C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 73, 361
AccB1I GGYRCC 3 cut(s) 73, 206, 361
AcyI GRCGYC 1 cut(s) 307
AfaI GTAC 2 cut(s) 75, 363
AgsI TTSAA 1 cut(s) 19
AhlI ACTAGT 1 cut(s) 253
AluBI AGCT 7 cut(s) 43, 110, 163, 182, 195, 317, 335
AluI AGCT 7 cut(s) 43, 110, 163, 182, 195, 317, 335
Ama87I CYCGRG 1 cut(s) 234
ApeKI GCWGC 2 cut(s) 179, 317
Asp718I GGTACC 2 cut(s) 73, 361
AsuC2I CCSGG 2 cut(s) 235, 236
AsuHPI GGTGA 1 cut(s) 302
AvaI CYCGRG 1 cut(s) 234
BaeGI GKGCMC 1 cut(s) 235
BanI GGYRCC 3 cut(s) 73, 206, 361
BbvI GCAGC 2 cut(s) 191, 304
BcnI CCSGG 2 cut(s) 235, 236
BcuI ACTAGT 1 cut(s) 253
BfaI CTAG 3 cut(s) 254, 297, 339
BisI GCNGC 2 cut(s) 180, 318
BlsI GCNGC 2 cut(s) 181, 319
Bme1390I CCNGG 2 cut(s) 235, 236
BmeT110I CYCGRG 1 cut(s) 234
BmiI GGNNCC 3 cut(s) 75, 208, 363
BmrFI CCNGG 2 cut(s) 235, 236
BmsI GCATC 1 cut(s) 166
Bpu10I CCTNAGC 1 cut(s) 183
BpuMI CCSGG 2 cut(s) 235, 236
BsaHI GRCGYC 1 cut(s) 307
BsaJI CCNNGG 1 cut(s) 234
BseDI CCNNGG 1 cut(s) 234
BseGI GGATG 1 cut(s) 10
BseMII CTCAG 1 cut(s) 155
BseRI GAGGAG 1 cut(s) 304
BseSI GKGCMC 1 cut(s) 235
BseXI GCAGC 2 cut(s) 191, 304
BshNI GGYRCC 3 cut(s) 73, 206, 361
BsiHKCI CYCGRG 1 cut(s) 234
BsiSI CCGG 1 cut(s) 235
BslFI GGGAC 1 cut(s) 275
BsmFI GGGAC 1 cut(s) 275
BsoBI CYCGRG 1 cut(s) 234
Bsp1286I GDGCHC 1 cut(s) 235
BspCNI CTCAG 1 cut(s) 156
BspLI GGNNCC 3 cut(s) 75, 208, 363
BspT107I GGYRCC 3 cut(s) 73, 206, 361
BssECI CCNNGG 1 cut(s) 234
BssNI GRCGYC 1 cut(s) 307
BstACI GRCGYC 1 cut(s) 307
BstC8I GCNNGC 1 cut(s) 45
BstDEI CTNAG 3 cut(s) 164, 183, 375
BstF5I GGATG 1 cut(s) 10
BstSCI CCNGG 2 cut(s) 233, 234
BstSLI GKGCMC 1 cut(s) 235
BstV1I GCAGC 2 cut(s) 191, 304
BtsCI GGATG 1 cut(s) 10
BtsIMutI CAGTG 1 cut(s) 391
Cac8I GCNNGC 1 cut(s) 45
Cfr9I CCCGGG 1 cut(s) 234
CseI GACGC 1 cut(s) 296
Csp6I GTAC 2 cut(s) 74, 362
CviAII CATG 2 cut(s) 242, 346
CviJI RGCY 8 cut(s) 43, 110, 163, 182, 195, 317, 335, 343
CviKI_1 RGCY 8 cut(s) 43, 110, 163, 182, 195, 317, 335, 343
CviQI GTAC 2 cut(s) 74, 362
DdeI CTNAG 3 cut(s) 164, 183, 375
DraI TTTAAA 1 cut(s) 36
Eco88I CYCGRG 1 cut(s) 234
FaeI CATG 2 cut(s) 245, 349
FaiI YATR 5 cut(s) 27, 243, 347, 352, 354
FaqI GGGAC 1 cut(s) 275
FatI CATG 2 cut(s) 241, 345
FauNDI CATATG 1 cut(s) 352
Fnu4HI GCNGC 2 cut(s) 180, 318
FokI GGATG 1 cut(s) 17
Fsp4HI GCNGC 2 cut(s) 180, 318
FspBI CTAG 3 cut(s) 254, 297, 339
GluI GCNGC 2 cut(s) 180, 318
HapII CCGG 1 cut(s) 235
HgaI GACGC 1 cut(s) 296
Hin1I GRCGYC 1 cut(s) 307
Hin1II CATG 2 cut(s) 245, 349
HindIII AAGCTT 1 cut(s) 41
HinfI GANTC 5 cut(s) 19, 151, 188, 263, 284
HpaII CCGG 1 cut(s) 235
HphI GGTGA 1 cut(s) 302
Hpy188I TCNGA 1 cut(s) 125
HpyAV CCTTC 2 cut(s) 64, 297
HpyCH4V TGCA 1 cut(s) 179
HpyF3I CTNAG 3 cut(s) 164, 183, 375
Hsp92I GRCGYC 1 cut(s) 307
Hsp92II CATG 2 cut(s) 245, 349
KpnI GGTACC 2 cut(s) 77, 365
LmnI GCTCC 1 cut(s) 52
LpnPI CCDG 4 cut(s) 198, 248, 345, 378
Lsp1109I GCAGC 2 cut(s) 191, 304
LweI GCATC 1 cut(s) 166
MaeI CTAG 3 cut(s) 254, 297, 339
MaeIII GTNAC 1 cut(s) 308
MboII GAAGA 1 cut(s) 312
MhlI GDGCHC 1 cut(s) 235
MluCI AATT 1 cut(s) 326
MlyI GAGTC 1 cut(s) 257
MnlI CCTC 1 cut(s) 282
MseI TTAA 1 cut(s) 35
MspI CCGG 1 cut(s) 235
MspR9I CCNGG 2 cut(s) 235, 236
NciI CCSGG 2 cut(s) 235, 236
NdeI CATATG 1 cut(s) 352
NlaIII CATG 2 cut(s) 245, 349
NlaIV GGNNCC 3 cut(s) 75, 208, 363
NmuCI GTSAC 1 cut(s) 308
PfeI GAWTC 4 cut(s) 19, 151, 188, 284
PkrI GCNGC 2 cut(s) 181, 319
PleI GAGTC 1 cut(s) 257
PpsI GAGTC 1 cut(s) 257
PspN4I GGNNCC 3 cut(s) 75, 208, 363
RsaI GTAC 2 cut(s) 75, 363
RsaNI GTAC 2 cut(s) 74, 362
SaqAI TTAA 1 cut(s) 35
SatI GCNGC 2 cut(s) 180, 318
SchI GAGTC 1 cut(s) 257
ScrFI CCNGG 2 cut(s) 235, 236
SduI GDGCHC 1 cut(s) 235
SetI ASST 9 cut(s) 45, 75, 112, 165, 184, 197, 319, 337, 367
SfaNI GCATC 1 cut(s) 166
SmaI CCCGGG 1 cut(s) 236
SpeI ACTAGT 1 cut(s) 253
Sse9I AATT 1 cut(s) 326
SspMI CTAG 3 cut(s) 254, 297, 339
StyD4I CCNGG 2 cut(s) 233, 234
TaqI TCGA 2 cut(s) 191, 287
TasI AATT 1 cut(s) 326
TfiI GAWTC 4 cut(s) 19, 151, 188, 284
Tru1I TTAA 1 cut(s) 35
Tru9I TTAA 1 cut(s) 35
TseFI GTSAC 1 cut(s) 308
TseI GCWGC 2 cut(s) 179, 317
Tsp45I GTSAC 1 cut(s) 308
TspDTI ATGAA 1 cut(s) 21
TspMI CCCGGG 1 cut(s) 234
XmaI CCCGGG 1 cut(s) 234
XspI CTAG 3 cut(s) 254, 297, 339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.