pycom04g21720

protein KRI1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0009859
Physical Location & Seq
Forward (+)
22736402 .. 22737226
825 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g21720.1

Sequence Viewer

Length: 825 bp
ATGGAGGAGGATGATGAGATTGAGAAACCGGACTTTGATAAGGAGGATGAGTTGCTTGGGCTTCCTAGAGGTTGGGATACCGTTGGGTCTAGTGATGGGTTCTTAGCTGCCAGGGAGAAGGTTTTGAAGCGGAAAAAGGAAAATGTTTTTGACCACGAGGAAGAGGAAGAGGAAGAGGAAGAGGAGGAGGAGGAGGAGGAGGAGGAGGAAGTAGAAGAAAAGGAAGAGGAAGAAGAAGAAGAAGAAATGAGTGAGGAAGATAAACAAGGGAAAAAGCGTAAATTAGCTTTATTGGAGAGAGCTAAGAAGGAGATGATGGATGAGTACTATAAGTTGGATTATGAGGATACAATTGGAGACTTGAAGACAAGATTCAAGTATGCTAAAATAAAACCTAATAGATATGGCTTGACTACTGCCGAGATATTAGCGATGGATGAAAAGGAGTTGAATCAATATGTGTCTTTAAAGAAGCTTGCTCCTTACACAGAGAAAGAGTGGAAGGTACCCAACAACAAGAGAATGGAGATAAAGCAGAAAGCTAAAGAGATTTTCAGACAAGGAAATGTGGGTAATAAAAAGAATCGCAAGAAGCTGAGAATAAGTGATGCAGCTAAGGATTCAAGCTTATCAAAGGTTGCCACGGGGCAATCATCAATGAGTGCCCTGGAACATGGGAAAGAACAAGTAGGGGACTCAAATGGTGGTAAAAAAGAATCGAGGAGTGCTAGAAGAAGGCGTCACCAAGCTGCAAGAAAATTACCAGCTTCTAGGCTCATGGCATATGGGGTGGTACCTGTAAAATCGAAGAAAAAAGGAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kri1 Kri1_C

Protein Analysis

275

Amino Acids

31.9

Weight (kDa)

5.35

Isoelectric Point (pI)

72.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kri1_C PF12936 102 - 184 8.3e-31 KRI1-like family C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 505, 793
AccB1I GGYRCC 2 cut(s) 505, 793
AciI CCGC 1 cut(s) 130
AcyI GRCGYC 1 cut(s) 739
AfaI GTAC 3 cut(s) 326, 507, 795
AgsI TTSAA 5 cut(s) 127, 364, 376, 451, 624
AjnI CCWGG 2 cut(s) 110, 666
Alw26I GTCTC 1 cut(s) 351
ApeKI GCWGC 3 cut(s) 107, 611, 749
Asp718I GGTACC 2 cut(s) 505, 793
AsuHPI GGTGA 1 cut(s) 734
BaeGI GKGCMC 1 cut(s) 667
BanI GGYRCC 2 cut(s) 505, 793
BauI CACGAG 1 cut(s) 155
BbsI GAAGAC 1 cut(s) 371
BbvI GCAGC 3 cut(s) 94, 623, 736
BccI CCATC 3 cut(s) 89, 310, 427
BciT130I CCWGG 2 cut(s) 112, 668
BciVI GTATCC 2 cut(s) 70, 340
BcoDI GTCTC 1 cut(s) 351
BfaI CTAG 4 cut(s) 66, 90, 729, 771
BfuI GTATCC 2 cut(s) 70, 340
BisI GCNGC 3 cut(s) 108, 612, 750
BlsI GCNGC 3 cut(s) 109, 613, 751
BmcAI AGTACT 1 cut(s) 326
Bme1390I CCNGG 2 cut(s) 112, 668
BmiI GGNNCC 2 cut(s) 507, 795
BmrFI CCNGG 2 cut(s) 112, 668
BmsI GCATC 1 cut(s) 598
BpiI GAAGAC 1 cut(s) 371
Bpu10I CCTNAGC 1 cut(s) 615
BsaHI GRCGYC 1 cut(s) 739
BsaJI CCNNGG 3 cut(s) 111, 642, 666
BsaWI WCCGGW 1 cut(s) 28
BsaXI ACNNNNNCTCC 2 cut(s) 35, 65
BseBI CCWGG 2 cut(s) 112, 668
BseDI CCNNGG 3 cut(s) 111, 642, 666
BseGI GGATG 4 cut(s) 16, 52, 325, 442
BseMII CTCAG 1 cut(s) 587
BseSI GKGCMC 1 cut(s) 667
BseXI GCAGC 3 cut(s) 94, 623, 736
BshNI GGYRCC 2 cut(s) 505, 793
BsiSI CCGG 1 cut(s) 29
BslFI GGGAC 1 cut(s) 707
BsmAI GTCTC 1 cut(s) 351
BsmFI GGGAC 1 cut(s) 707
Bsp1286I GDGCHC 1 cut(s) 667
BspACI CCGC 1 cut(s) 130
BspCNI CTCAG 1 cut(s) 588
BspLI GGNNCC 2 cut(s) 507, 795
BspT107I GGYRCC 2 cut(s) 505, 793
BssECI CCNNGG 3 cut(s) 111, 642, 666
BssNI GRCGYC 1 cut(s) 739
BssSI CACGAG 1 cut(s) 155
Bst2BI CACGAG 1 cut(s) 155
Bst2UI CCWGG 2 cut(s) 112, 668
Bst4CI ACNGT 1 cut(s) 82
Bst6I CTCTTC 5 cut(s) 156, 162, 168, 174, 219
BstACI GRCGYC 1 cut(s) 739
BstC8I GCNNGC 1 cut(s) 477
BstDEI CTNAG 4 cut(s) 103, 303, 596, 615
BstDSI CCRYGG 1 cut(s) 642
BstF5I GGATG 4 cut(s) 16, 52, 325, 442
BstMAI GTCTC 1 cut(s) 351
BstNI CCWGG 2 cut(s) 112, 668
BstSCI CCNGG 2 cut(s) 110, 666
BstSLI GKGCMC 1 cut(s) 667
BstV1I GCAGC 3 cut(s) 94, 623, 736
BstV2I GAAGAC 1 cut(s) 371
BsuI GTATCC 2 cut(s) 70, 340
BtgI CCRYGG 1 cut(s) 642
BtgZI GCGATG 1 cut(s) 446
BtsCI GGATG 4 cut(s) 16, 52, 325, 442
Cac8I GCNNGC 1 cut(s) 477
CseI GACGC 1 cut(s) 728
Csp6I GTAC 3 cut(s) 325, 506, 794
CviAII CATG 2 cut(s) 674, 778
CviQI GTAC 3 cut(s) 325, 506, 794
DdeI CTNAG 4 cut(s) 103, 303, 596, 615
DraI TTTAAA 1 cut(s) 468
Eam1104I CTCTTC 5 cut(s) 156, 162, 168, 174, 219
EarI CTCTTC 5 cut(s) 156, 162, 168, 174, 219
EcoRII CCWGG 2 cut(s) 110, 666
FaeI CATG 2 cut(s) 677, 781
FaiI YATR 9 cut(s) 330, 342, 381, 405, 459, 675, 779, 784, 786
FaqI GGGAC 1 cut(s) 707
FatI CATG 2 cut(s) 673, 777
FauNDI CATATG 1 cut(s) 784
Fnu4HI GCNGC 3 cut(s) 108, 612, 750
FokI GGATG 4 cut(s) 23, 59, 332, 449
Fsp4HI GCNGC 3 cut(s) 108, 612, 750
FspBI CTAG 4 cut(s) 66, 90, 729, 771
GluI GCNGC 3 cut(s) 108, 612, 750
HapII CCGG 1 cut(s) 29
HgaI GACGC 1 cut(s) 728
Hin1I GRCGYC 1 cut(s) 739
Hin1II CATG 2 cut(s) 677, 781
HindIII AAGCTT 2 cut(s) 473, 625
HinfI GANTC 6 cut(s) 372, 451, 583, 620, 695, 716
HpaII CCGG 1 cut(s) 29
HphI GGTGA 1 cut(s) 734
Hpy188I TCNGA 1 cut(s) 557
HpyAV CCTTC 4 cut(s) 112, 301, 496, 729
HpyCH4III ACNGT 1 cut(s) 82
HpyCH4V TGCA 2 cut(s) 611, 752
HpyF3I CTNAG 4 cut(s) 103, 303, 596, 615
Hsp92I GRCGYC 1 cut(s) 739
Hsp92II CATG 2 cut(s) 677, 781
KpnI GGTACC 2 cut(s) 509, 797
LmnI GCTCC 1 cut(s) 484
LpnPI CCDG 7 cut(s) 42, 97, 124, 653, 680, 777, 810
Lsp1109I GCAGC 3 cut(s) 94, 623, 736
LweI GCATC 1 cut(s) 598
MaeI CTAG 4 cut(s) 66, 90, 729, 771
MaeIII GTNAC 1 cut(s) 740
MfeI CAATTG 1 cut(s) 351
MhlI GDGCHC 1 cut(s) 667
MluCI AATT 3 cut(s) 281, 351, 758
MlyI GAGTC 1 cut(s) 689
MmeI TCCRAC 1 cut(s) 315
MseI TTAA 1 cut(s) 467
MspI CCGG 1 cut(s) 29
MspR9I CCNGG 2 cut(s) 112, 668
MunI CAATTG 1 cut(s) 351
MvaI CCWGG 2 cut(s) 112, 668
NdeI CATATG 1 cut(s) 784
NlaIII CATG 2 cut(s) 677, 781
NlaIV GGNNCC 2 cut(s) 507, 795
NmeAIII GCCGAG 1 cut(s) 445
NmuCI GTSAC 1 cut(s) 740
PfeI GAWTC 5 cut(s) 372, 451, 583, 620, 716
PkrI GCNGC 3 cut(s) 109, 613, 751
PleI GAGTC 1 cut(s) 689
PpsI GAGTC 1 cut(s) 689
Psp6I CCWGG 2 cut(s) 110, 666
PspGI CCWGG 2 cut(s) 110, 666
PspN4I GGNNCC 2 cut(s) 507, 795
RsaI GTAC 3 cut(s) 326, 507, 795
RsaNI GTAC 3 cut(s) 325, 506, 794
SaqAI TTAA 1 cut(s) 467
SatI GCNGC 3 cut(s) 108, 612, 750
ScaI AGTACT 1 cut(s) 326
SchI GAGTC 1 cut(s) 689
ScrFI CCNGG 2 cut(s) 112, 668
SduI GDGCHC 1 cut(s) 667
SfaNI GCATC 1 cut(s) 598
Sse9I AATT 3 cut(s) 281, 351, 758
SsiI CCGC 1 cut(s) 130
SspMI CTAG 4 cut(s) 66, 90, 729, 771
StyD4I CCNGG 2 cut(s) 110, 666
TaaI ACNGT 1 cut(s) 82
TaqI TCGA 2 cut(s) 719, 806
TasI AATT 3 cut(s) 281, 351, 758
TatI WGTACW 1 cut(s) 324
TfiI GAWTC 5 cut(s) 372, 451, 583, 620, 716
Tru1I TTAA 1 cut(s) 467
Tru9I TTAA 1 cut(s) 467
TseFI GTSAC 1 cut(s) 740
TseI GCWGC 3 cut(s) 107, 611, 749
Tsp45I GTSAC 1 cut(s) 740
TspDTI ATGAA 1 cut(s) 453
XspI CTAG 4 cut(s) 66, 90, 729, 771
ZrmI AGTACT 1 cut(s) 326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.