MD05G1012600.v1.1

Exostosin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0002953
Physical Location & Seq
Reverse (-)
2491757 .. 2496333
4577 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1012600.v1.1.491

Sequence Viewer

Length: 1524 bp
ATGAATGTTGATTATAGGACTGGGACAGGGAGGGGACATGTAATGTTGGGACATAAGACCTTAACTCGTTCCCTTCTTCCCCAGAAGCCGAGCTGCAGAAGAACCCCACTGCCATCCAGCACTGATGATACTCTGAAAATGGGAAGCGTAAACAACAAAGCTCGACCATTCAGCACGCAGCACCACCAGCATCCTCTGTGCACCCGCACGCACCAGATCGGAGCCCTCCTCTTGGTCGCCGCCTCCTTCTTCTTGACTAGCCTCTTAGACCGCTCCTTCCGCCCCTGCGCCTCTCACTTCGCCGCCGTCGATATCTTCCCCAATTCACGGAGCTCCGTCCAAGTCACCGGCGGTCGAGATCTCTTGTGGCCGCACCGAGGGTACGGCCCCCTACTCGACCTCAAAATCTACGTCTACGATGACAGCGAGATCGATGGCCTCAAGGCATTGATGCGCGGTCGCGATGGTGTCATCGACTCCAACGCTTGCTTGAAAGGCCAATGGGGCACTCAGGTTAAAATACACAGGCTACTGCTAAATTCGAGGTTTCGGACAAGGAAGAAAGAGGAAGCAGACCTATTTTTTGTGCCTACGTATACTAAATGCGTTCGGATGATGGGGGGTCTTAATGATAAGGAGATTAACCAGACATATGTGAAGGTGTTAAGTCAAATGCCATATTTCAGGCGATCTGGTGGCCGTGACCACATATTTGTTTTTTCAAGTGGTGCTGGAGCTCACTTATTTAGATCCTGGGCAACATATATAAATCGTTCCATTATTCTCACCCCTGAGGGCGATCGGACTGATAAGAAAGATACAAGTGCCTTTAATACGTGGAAAGATATCATAATTCCTGGGAATGTTGAGGACGGTATGACTACAAATGGGGCCACCTTGGTCCACCCTTTGCCTATACCAAAGCGGAAGTATCTAGCAAACTATTTAGGCCGTGCACAAGGAAAGGTTGGCCGTCTTAAGTTGATAGAGCTTTCAAGGAAATTTCCAGATAAGTTGGAATGTCCAGAGTTGAAGTTCAGTGGTCCTGAAAAATTTGGAAGAATAGATTATTTTGAACACCTGCGCGACGCCAAGTTCTGCCTTGCTCCACGTGGGGAGTCATCCTGGACTCTTCGATTTTATGAGGCTTTCTTTGTGGAGTGTGTACCTGTGATATTATCAGATCAAGTAGAACTGCCTTTCCAAAATGTTGTTGACTACACCCAGATATCAATTAAATGGCCATCCACTCGAATAGGTCCCGAGCTTTTGCAGTACCTAGAGTCAATACCAGATGAATATATAGAAGGGATGATAAGCCGGGGCAGACAAGTACGATGTTTATGGGTCTATGCTTCAGAATCAGGTTCATGTTCGGCAATGCATGCGATTCTTTGGGAGCTTCAGAGGAAAGTAAGACTGTTTCACCAGTCTACGGAAACATTCTGGTTGCACAATGGATCTGTTGTAAATAGAAACTTGGTAGAATTTTCGGAGTGGAGATTGCCAATGCCTTTGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exostosin NAM

Protein Analysis

508

Amino Acids

57.91

Weight (kDa)

9.45

Isoelectric Point (pI)

42.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exostosin_GT47 PF03016 134 - 419 3.7e-42 Exostosin GT47 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1089
Acc36I ACCTGC 1 cut(s) 1089
AccBSI CCGCTC 1 cut(s) 273
AccI GTMKAC 3 cut(s) 414, 596, 1433
AccII CGCG 3 cut(s) 456, 462, 1086
AciI CCGC 9 cut(s) 205, 240, 271, 280, 303, 351, 371, 456, 925
AclWI GGATC 2 cut(s) 744, 1468
AcoI YGGCCR 4 cut(s) 368, 697, 970, 1241
AcsI RAATTY 4 cut(s) 538, 1001, 1052, 1487
AcuI CTGAAG 2 cut(s) 1341, 1388
AcvI CACGTG 1 cut(s) 1112
AcyI GRCGYC 1 cut(s) 1089
AfaI GTAC 4 cut(s) 383, 1167, 1277, 1335
AfiI CCNNNNNNNGG 4 cut(s) 232, 327, 377, 1435
AflII CTTAAG 1 cut(s) 977
AflIII ACRYGT 1 cut(s) 37
AgsI TTSAA 5 cut(s) 493, 723, 996, 1033, 1076
AjnI CCWGG 3 cut(s) 752, 856, 1124
AloI GAACNNNNNNTCC 2 cut(s) 1185, 1217
AluBI AGCT 7 cut(s) 93, 161, 333, 737, 991, 1267, 1402
AluI AGCT 7 cut(s) 93, 161, 333, 737, 991, 1267, 1402
Alw21I GWGCWC 4 cut(s) 203, 335, 739, 958
Alw44I GTGCAC 2 cut(s) 199, 954
AlwI GGATC 2 cut(s) 744, 1468
Ama87I CYCGRG 1 cut(s) 1262
AoxI GGCC 9 cut(s) 368, 385, 436, 496, 697, 891, 949, 970, 1241
ApaLI GTGCAC 2 cut(s) 199, 954
ApeKI GCWGC 2 cut(s) 93, 178
ApoI RAATTY 4 cut(s) 538, 1001, 1052, 1487
ArsI GACNNNNNNTTYG 6 cut(s) 396, 428, 566, 598, 695, 727
AspLEI GCGC 3 cut(s) 290, 456, 1086
AspS9I GGNCC 5 cut(s) 386, 891, 901, 1043, 1259
AsuC2I CCSGG 1 cut(s) 1322
AsuHPI GGTGA 3 cut(s) 337, 778, 1418
AvaI CYCGRG 1 cut(s) 1262
AvaII GGWCC 3 cut(s) 901, 1043, 1259
AxyI CCTNAGG 1 cut(s) 792
BaeGI GKGCMC 3 cut(s) 203, 509, 958
BalI TGGCCA 1 cut(s) 1243
BanII GRGCYC 3 cut(s) 226, 335, 739
BbrPI CACGTG 1 cut(s) 1112
Bbv12I GWGCWC 4 cut(s) 203, 335, 739, 958
BbvI GCAGC 2 cut(s) 80, 190
BccI CCATC 5 cut(s) 121, 428, 458, 610, 1252
BceAI ACGGC 5 cut(s) 290, 400, 684, 936, 957
BciT130I CCWGG 3 cut(s) 754, 858, 1126
BcnI CCSGG 1 cut(s) 1322
BfaI CTAG 3 cut(s) 258, 935, 1280
BfmI CTRYAG 1 cut(s) 94
BfrI CTTAAG 1 cut(s) 977
BfuAI ACCTGC 1 cut(s) 1089
BglI GCCNNNNNGGC 1 cut(s) 504
BglII AGATCT 1 cut(s) 358
BisI GCNGC 5 cut(s) 94, 179, 240, 303, 371
BlsI GCNGC 5 cut(s) 95, 180, 241, 304, 372
Bme1390I CCNGG 4 cut(s) 754, 858, 1126, 1322
Bme18I GGWCC 3 cut(s) 901, 1043, 1259
BmeT110I CYCGRG 1 cut(s) 1262
BmgT120I GGNCC 5 cut(s) 386, 891, 901, 1043, 1259
BmiI GGNNCC 4 cut(s) 223, 388, 892, 1261
BmrFI CCNGG 4 cut(s) 754, 858, 1126, 1322
BmrI ACTGGG 1 cut(s) 30
BmsI GCATC 2 cut(s) 199, 441
BmuI ACTGGG 1 cut(s) 30
BplI GAGNNNNNCTC 2 cut(s) 213, 245
BpmI CTGGAG 1 cut(s) 753
BpuEI CTTGAG 1 cut(s) 425
BpuMI CCSGG 1 cut(s) 1322
Bsa29I ATCGAT 1 cut(s) 432
BsaAI YACGTR 3 cut(s) 594, 837, 1112
BsaHI GRCGYC 1 cut(s) 1089
BsaJI CCNNGG 5 cut(s) 376, 753, 857, 897, 1321
Bsc4I CCNNNNNNNGG 4 cut(s) 232, 327, 377, 1435
Bse118I RCCGGY 1 cut(s) 347
Bse1I ACTGG 2 cut(s) 25, 1429
Bse21I CCTNAGG 1 cut(s) 792
Bse3DI GCAATG 1 cut(s) 1386
BseBI CCWGG 3 cut(s) 754, 858, 1126
BseCI ATCGAT 1 cut(s) 432
BseDI CCNNGG 5 cut(s) 376, 753, 857, 897, 1321
BseGI GGATG 6 cut(s) 113, 190, 618, 1121, 1244, 1317
BseLI CCNNNNNNNGG 4 cut(s) 232, 327, 377, 1435
BseMI GCAATG 1 cut(s) 1386
BseMII CTCAG 2 cut(s) 524, 783
BseNI ACTGG 2 cut(s) 25, 1429
BseRI GAGGAG 1 cut(s) 218
BseSI GKGCMC 3 cut(s) 203, 509, 958
BseXI GCAGC 2 cut(s) 80, 190
Bsh1236I CGCG 3 cut(s) 456, 462, 1086
Bsh1285I CGRYCG 3 cut(s) 355, 460, 802
BshFI GGCC 9 cut(s) 370, 387, 438, 498, 699, 893, 951, 972, 1243
BshVI ATCGAT 1 cut(s) 432
BsiEI CGRYCG 3 cut(s) 355, 460, 802
BsiHKAI GWGCWC 4 cut(s) 203, 335, 739, 958
BsiHKCI CYCGRG 1 cut(s) 1262
BsiSI CCGG 2 cut(s) 348, 1321
BslFI GGGAC 4 cut(s) 37, 48, 63, 1245
BslI CCNNNNNNNGG 4 cut(s) 232, 327, 377, 1435
BsmFI GGGAC 4 cut(s) 37, 48, 63, 1245
BsnI GGCC 9 cut(s) 370, 387, 438, 498, 699, 893, 951, 972, 1243
BsoBI CYCGRG 1 cut(s) 1262
Bsp1286I GDGCHC 6 cut(s) 203, 226, 335, 509, 739, 958
Bsp143I GATC 8 cut(s) 216, 358, 429, 689, 749, 799, 1183, 1460
Bsp68I TCGCGA 1 cut(s) 462
BspACI CCGC 9 cut(s) 205, 240, 271, 280, 303, 351, 371, 456, 925
BspANI GGCC 9 cut(s) 370, 387, 438, 498, 699, 893, 951, 972, 1243
BspCNI CTCAG 2 cut(s) 523, 784
BspDI ATCGAT 1 cut(s) 432
BspFNI CGCG 3 cut(s) 456, 462, 1086
BspLI GGNNCC 4 cut(s) 223, 388, 892, 1261
BspMAI CTGCAG 1 cut(s) 98
BspMI ACCTGC 1 cut(s) 1089
BspPI GGATC 2 cut(s) 744, 1468
BspTI CTTAAG 1 cut(s) 977
BsrBI CCGCTC 1 cut(s) 273
BsrDI GCAATG 1 cut(s) 1386
BsrFI RCCGGY 1 cut(s) 347
BsrI ACTGG 2 cut(s) 25, 1429
BssAI RCCGGY 1 cut(s) 347
BssECI CCNNGG 5 cut(s) 376, 753, 857, 897, 1321
BssMI GATC 8 cut(s) 216, 358, 429, 689, 749, 799, 1183, 1460
BssNAI GTATAC 1 cut(s) 597
BssNI GRCGYC 1 cut(s) 1089
BssT1I CCWWGG 1 cut(s) 897
Bst1107I GTATAC 1 cut(s) 597
Bst2UI CCWGG 3 cut(s) 754, 858, 1126
Bst4CI ACNGT 2 cut(s) 875, 1422
Bst6I CTCTTC 1 cut(s) 1137
BstACI GRCGYC 1 cut(s) 1089
BstAFI CTTAAG 1 cut(s) 977
BstAPI GCANNNNNTGC 1 cut(s) 1385
BstBAI YACGTR 3 cut(s) 594, 837, 1112
BstC8I GCNNGC 4 cut(s) 176, 209, 487, 1386
BstDEI CTNAG 3 cut(s) 265, 510, 792
BstF5I GGATG 6 cut(s) 113, 190, 618, 1121, 1244, 1317
BstFNI CGCG 3 cut(s) 456, 462, 1086
BstHHI GCGC 3 cut(s) 290, 456, 1086
BstKTI GATC 8 cut(s) 219, 361, 432, 692, 752, 802, 1186, 1463
BstMBI GATC 8 cut(s) 216, 358, 429, 689, 749, 799, 1183, 1460
BstMCI CGRYCG 3 cut(s) 355, 460, 802
BstMWI GCNNNNNNNGC 5 cut(s) 187, 279, 495, 504, 1385
BstNI CCWGG 3 cut(s) 754, 858, 1126
BstNSI RCATGY 2 cut(s) 41, 1388
BstSCI CCNGG 4 cut(s) 752, 856, 1124, 1320
BstSFI CTRYAG 1 cut(s) 94
BstSLI GKGCMC 3 cut(s) 203, 509, 958
BstSNI TACGTA 1 cut(s) 594
BstUI CGCG 3 cut(s) 456, 462, 1086
BstV1I GCAGC 2 cut(s) 80, 190
BstX2I RGATCY 3 cut(s) 358, 749, 1460
BstYI RGATCY 3 cut(s) 358, 749, 1460
BstZ17I GTATAC 1 cut(s) 597
Bsu15I ATCGAT 1 cut(s) 432
Bsu36I CCTNAGG 1 cut(s) 792
BsuRI GGCC 9 cut(s) 370, 387, 438, 498, 699, 893, 951, 972, 1243
BsuTUI ATCGAT 1 cut(s) 432
BtgZI GCGATG 1 cut(s) 477
BtsCI GGATG 6 cut(s) 113, 190, 618, 1121, 1244, 1317
BtsI GCAGTG 1 cut(s) 107
BtsIMutI CAGTG 3 cut(s) 107, 120, 1045
BtuMI TCGCGA 1 cut(s) 462
BveI ACCTGC 1 cut(s) 1089
Cac8I GCNNGC 4 cut(s) 176, 209, 487, 1386
CfoI GCGC 3 cut(s) 290, 456, 1086
Cfr10I RCCGGY 1 cut(s) 347
Cfr13I GGNCC 5 cut(s) 386, 891, 901, 1043, 1259
ClaI ATCGAT 1 cut(s) 432
CseI GACGC 1 cut(s) 1097
Csp6I GTAC 4 cut(s) 382, 1166, 1276, 1334
CviAII CATG 3 cut(s) 38, 1371, 1385
CviQI GTAC 4 cut(s) 382, 1166, 1276, 1334
DdeI CTNAG 3 cut(s) 265, 510, 792
DpnI GATC 8 cut(s) 218, 360, 431, 691, 751, 801, 1185, 1462
DpnII GATC 8 cut(s) 216, 358, 429, 689, 749, 799, 1183, 1460
EaeI YGGCCR 4 cut(s) 368, 697, 970, 1241
Eam1104I CTCTTC 1 cut(s) 1137
EarI CTCTTC 1 cut(s) 1137
EciI GGCGGA 1 cut(s) 269
Ecl136II GAGCTC 2 cut(s) 333, 737
Eco105I TACGTA 1 cut(s) 594
Eco130I CCWWGG 1 cut(s) 897
Eco24I GRGCYC 3 cut(s) 226, 335, 739
Eco32I GATATC 3 cut(s) 313, 847, 1230
Eco47I GGWCC 3 cut(s) 901, 1043, 1259
Eco53kI GAGCTC 2 cut(s) 333, 737
Eco57I CTGAAG 2 cut(s) 1341, 1388
Eco72I CACGTG 1 cut(s) 1112
Eco81I CCTNAGG 1 cut(s) 792
Eco88I CYCGRG 1 cut(s) 1262
EcoICRI GAGCTC 2 cut(s) 333, 737
EcoO109I RGGNCCY 1 cut(s) 1259
EcoRII CCWGG 3 cut(s) 752, 856, 1124
EcoRV GATATC 3 cut(s) 313, 847, 1230
EcoT14I CCWWGG 1 cut(s) 897
EcoT22I ATGCAT 1 cut(s) 1386
EcoT38I GRGCYC 3 cut(s) 226, 335, 739
ErhI CCWWGG 1 cut(s) 897
FaeI CATG 3 cut(s) 41, 1374, 1388
FaqI GGGAC 4 cut(s) 37, 48, 63, 1245
FatI CATG 3 cut(s) 37, 1370, 1384
FauI CCCGC 1 cut(s) 212
FauNDI CATATG 1 cut(s) 652
FblI GTMKAC 3 cut(s) 414, 596, 1433
Fnu4HI GCNGC 5 cut(s) 94, 179, 240, 303, 371
FokI GGATG 6 cut(s) 100, 177, 625, 1108, 1231, 1324
FriOI GRGCYC 3 cut(s) 226, 335, 739
Fsp4HI GCNGC 5 cut(s) 94, 179, 240, 303, 371
FspBI CTAG 3 cut(s) 258, 935, 1280
GlaI GCGC 3 cut(s) 289, 455, 1085
GluI GCNGC 5 cut(s) 94, 179, 240, 303, 371
GsuI CTGGAG 1 cut(s) 753
HaeIII GGCC 9 cut(s) 370, 387, 438, 498, 699, 893, 951, 972, 1243
HapII CCGG 2 cut(s) 348, 1321
HgaI GACGC 1 cut(s) 1097
HhaI GCGC 3 cut(s) 290, 456, 1086
Hin1I GRCGYC 1 cut(s) 1089
Hin1II CATG 3 cut(s) 41, 1374, 1388
Hin6I GCGC 3 cut(s) 288, 454, 1084
HinP1I GCGC 3 cut(s) 288, 454, 1084
HincII GTYRAC 1 cut(s) 1216
HindII GTYRAC 1 cut(s) 1216
HinfI GANTC 6 cut(s) 476, 1118, 1129, 1283, 1361, 1390
HpaII CCGG 2 cut(s) 348, 1321
HphI GGTGA 3 cut(s) 337, 778, 1418
Hpy166II GTNNAC 9 cut(s) 151, 201, 415, 597, 904, 956, 1166, 1216, 1434
Hpy188I TCNGA 9 cut(s) 135, 221, 552, 612, 804, 1183, 1360, 1407, 1495
Hpy188III TCNNGA 7 cut(s) 253, 356, 461, 1007, 1025, 1046, 1262
Hpy8I GTNNAC 9 cut(s) 151, 201, 415, 597, 904, 956, 1166, 1216, 1434
Hpy99I CGWCG 2 cut(s) 311, 1091
HpyAV CCTTC 5 cut(s) 83, 256, 286, 652, 1301
HpyCH4III ACNGT 2 cut(s) 875, 1422
HpyCH4IV ACGT 4 cut(s) 411, 593, 836, 1111
HpyCH4V TGCA 6 cut(s) 96, 201, 956, 1273, 1384, 1453
HpyF10VI GCNNNNNNNGC 5 cut(s) 187, 279, 495, 504, 1385
HpyF3I CTNAG 3 cut(s) 265, 510, 792
HpySE526I ACGT 4 cut(s) 411, 593, 836, 1111
Hsp92I GRCGYC 1 cut(s) 1089
Hsp92II CATG 3 cut(s) 41, 1374, 1388
HspAI GCGC 3 cut(s) 288, 454, 1084
Kzo9I GATC 8 cut(s) 216, 358, 429, 689, 749, 799, 1183, 1460
LmnI GCTCC 7 cut(s) 221, 278, 330, 338, 734, 1111, 1399
Lsp1109I GCAGC 2 cut(s) 80, 190
LweI GCATC 2 cut(s) 199, 441
MaeI CTAG 3 cut(s) 258, 935, 1280
MaeII ACGT 4 cut(s) 411, 593, 836, 1111
MaeIII GTNAC 2 cut(s) 343, 701
MalI GATC 8 cut(s) 218, 360, 431, 691, 751, 801, 1185, 1462
MbiI CCGCTC 1 cut(s) 273
MboI GATC 8 cut(s) 216, 358, 429, 689, 749, 799, 1183, 1460
MboII GAAGA 7 cut(s) 68, 111, 241, 307, 571, 1071, 1124
MflI RGATCY 3 cut(s) 358, 749, 1460
MhlI GDGCHC 6 cut(s) 203, 226, 335, 509, 739, 958
MlsI TGGCCA 1 cut(s) 1243
MluCI AATT 7 cut(s) 322, 538, 852, 1001, 1052, 1233, 1487
MluNI TGGCCA 1 cut(s) 1243
MlyI GAGTC 4 cut(s) 470, 1123, 1127, 1292
MmeI TCCRAC 2 cut(s) 504, 996
Mox20I TGGCCA 1 cut(s) 1243
Mph1103I ATGCAT 1 cut(s) 1386
MscI TGGCCA 1 cut(s) 1243
MseI TTAA 8 cut(s) 62, 516, 627, 642, 665, 831, 978, 1236
Msp20I TGGCCA 1 cut(s) 1243
MspCI CTTAAG 1 cut(s) 977
MspI CCGG 2 cut(s) 348, 1321
MspR9I CCNGG 4 cut(s) 754, 858, 1126, 1322
MvaI CCWGG 3 cut(s) 754, 858, 1126
MvnI CGCG 3 cut(s) 456, 462, 1086
MwoI GCNNNNNNNGC 5 cut(s) 187, 279, 495, 504, 1385
NciI CCSGG 1 cut(s) 1322
NdeI CATATG 1 cut(s) 652
NdeII GATC 8 cut(s) 216, 358, 429, 689, 749, 799, 1183, 1460
NlaIII CATG 3 cut(s) 41, 1374, 1388
NlaIV GGNNCC 4 cut(s) 223, 388, 892, 1261
NmeAIII GCCGAG 1 cut(s) 114
NmuCI GTSAC 2 cut(s) 343, 701
NruI TCGCGA 1 cut(s) 462
NsiI ATGCAT 1 cut(s) 1386
NspI RCATGY 2 cut(s) 41, 1388
PaeI GCATGC 1 cut(s) 1388
PaqCI CACCTGC 1 cut(s) 1089
PciI ACATGT 1 cut(s) 37
PcsI WCGNNNNNNNCGW 2 cut(s) 306, 423
PfeI GAWTC 2 cut(s) 1361, 1390
PfoI TCCNGGA 1 cut(s) 1124
PkrI GCNGC 5 cut(s) 95, 180, 241, 304, 372
Ple19I CGATCG 1 cut(s) 802
PleI GAGTC 4 cut(s) 470, 1123, 1126, 1291
PmaCI CACGTG 1 cut(s) 1112
PmlI CACGTG 1 cut(s) 1112
PpsI GAGTC 4 cut(s) 470, 1123, 1126, 1291
Ppu21I YACGTR 3 cut(s) 594, 837, 1112
PpuMI RGGWCCY 1 cut(s) 1259
PscI ACATGT 1 cut(s) 37
Psp124BI GAGCTC 2 cut(s) 335, 739
Psp5II RGGWCCY 1 cut(s) 1259
Psp6I CCWGG 3 cut(s) 752, 856, 1124
PspCI CACGTG 1 cut(s) 1112
PspGI CCWGG 3 cut(s) 752, 856, 1124
PspN4I GGNNCC 4 cut(s) 223, 388, 892, 1261
PspPI GGNCC 5 cut(s) 386, 891, 901, 1043, 1259
PspPPI RGGWCCY 1 cut(s) 1259
PstI CTGCAG 1 cut(s) 98
PsuI RGATCY 3 cut(s) 358, 749, 1460
PvuI CGATCG 1 cut(s) 802
RruI TCGCGA 1 cut(s) 462
RsaI GTAC 4 cut(s) 383, 1167, 1277, 1335
RsaNI GTAC 4 cut(s) 382, 1166, 1276, 1334
SacI GAGCTC 2 cut(s) 335, 739
SaqAI TTAA 8 cut(s) 62, 516, 627, 642, 665, 831, 978, 1236
SatI GCNGC 5 cut(s) 94, 179, 240, 303, 371
Sau3AI GATC 8 cut(s) 216, 358, 429, 689, 749, 799, 1183, 1460
Sau96I GGNCC 5 cut(s) 386, 891, 901, 1043, 1259
SchI GAGTC 4 cut(s) 470, 1123, 1127, 1292
ScrFI CCNGG 4 cut(s) 754, 858, 1126, 1322
SduI GDGCHC 6 cut(s) 203, 226, 335, 509, 739, 958
SfaNI GCATC 2 cut(s) 199, 441
SfcI CTRYAG 1 cut(s) 94
SgrAI CRCCGGYG 1 cut(s) 347
SinI GGWCC 3 cut(s) 901, 1043, 1259
SmlI CTYRAG 2 cut(s) 440, 977
SmoI CTYRAG 2 cut(s) 440, 977
SnaBI TACGTA 1 cut(s) 594
SphI GCATGC 1 cut(s) 1388
Sse9I AATT 7 cut(s) 322, 538, 852, 1001, 1052, 1233, 1487
SsiI CCGC 9 cut(s) 205, 240, 271, 280, 303, 351, 371, 456, 925
SspMI CTAG 3 cut(s) 258, 935, 1280
SstI GAGCTC 2 cut(s) 335, 739
StyD4I CCNGG 4 cut(s) 752, 856, 1124, 1320
StyI CCWWGG 1 cut(s) 897
TaaI ACNGT 2 cut(s) 875, 1422
TaiI ACGT 4 cut(s) 414, 596, 839, 1114
TaqI TCGA 9 cut(s) 163, 309, 355, 396, 432, 474, 542, 1135, 1252
TasI AATT 7 cut(s) 322, 538, 852, 1001, 1052, 1233, 1487
TauI GCSGC 3 cut(s) 242, 305, 373
TfiI GAWTC 2 cut(s) 1361, 1390
Tru1I TTAA 8 cut(s) 62, 516, 627, 642, 665, 831, 978, 1236
Tru9I TTAA 8 cut(s) 62, 516, 627, 642, 665, 831, 978, 1236
TscAI CASTG 3 cut(s) 114, 127, 1045
TseFI GTSAC 2 cut(s) 343, 701
TseI GCWGC 2 cut(s) 93, 178
Tsp45I GTSAC 2 cut(s) 343, 701
TspDTI ATGAA 3 cut(s) 17, 1311, 1359
TspGWI ACGGA 3 cut(s) 325, 343, 1451
TspRI CASTG 3 cut(s) 114, 127, 1045
Vha464I CTTAAG 1 cut(s) 977
VneI GTGCAC 2 cut(s) 199, 954
VpaK11BI GGWCC 3 cut(s) 901, 1043, 1259
XapI RAATTY 4 cut(s) 538, 1001, 1052, 1487
XceI RCATGY 2 cut(s) 41, 1388
XmiI GTMKAC 3 cut(s) 414, 596, 1433
XspI CTAG 3 cut(s) 258, 935, 1280
Zsp2I ATGCAT 1 cut(s) 1386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.