MD10G1012500.v1.1

Exostosin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0002953
Physical Location & Seq
Reverse (-)
1714773 .. 1719692
4920 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1012500.v1.1.491

Sequence Viewer

Length: 1386 bp
ATGGGAAGCGTAAGCAACAAACCCCGACCATTCAGCGCGCAGCACCACCAGCAGCCTCTCTGCACCCGCACGCACCAGATCGGAGCCCTCCTCTTGGTCGCCGCCACCTTCTTCTTCACCAGTCTCTTAGACCGCTCCTTCCGCCCCTGCGCCTCTCACTTCCCCGCCGTAGATCTCCTCCCCAATTCACGGAGCTCCGTCCAAGTCACCGGCGGCCGAGATCTCTTGTGGCCCCAACGAGGGTACGGCCCCCAACTCGACCTCAAGATCTACGTCTACGATGCCCGCGAGATCGACGGCCTCAAGGCATTGATGCGTGGTCGCGATGGTGTCATCGACCCCAAGGCTTGCTTGAAAGGCCAGTGGGGCACGCAAGTTAAAATACACAGGCTACTGCTAAATTCGAGATTCCGGACAAGGAAGAAAGATGAAGCAGACCTATTTTTTGTGCCTACATATACTAAATGCGTTCGGATGATGGGGGGTCTTAATGATAAGGAGATTAACCAGACATATGTGAAGGTGTTAAGTCAAATGCCGGATTTCAGGCGATCTGGTGGCCGTGACCACATTTTCGTATTTCCAAGTGGTGCTGGAGCTCACTTATTTAGATCTTGGGCAACATTTATAAATCGTTCCATTATTCTTACCCCTGAGGGTGATCGGACTGATAAGAAAGATACAAGTGCCTTTAATACATGGAAAGATATCATAATTCCCGGAAATGTTGAGGACGGTATGACTACAAACGGGCCCACCTTGGTCCACCCTTTGCCCATATCAAAGAGGAAGTATCTGGCAAACTATTTAGGCCGTGCACAAGGAAAGGCTGGCCGTCTTAAGTTGATAGAGCTTTCAAAGAAATTTCCAGATAAGTTGGAATGTCCAGAGTTGAAGTTCAGTGGCCCTGAAAAATTGGGAAGGATAGATTACTTTGAACACCTGCGCAATGCCAAGTTCTGTCTTGCCCCACGTGGGGAGTCATCATGGACTCTTCGATTTTACGAGGCTTTTTTTGTGGAGTGTGTCCCTGTGCTATTATCAGATCAAGTGGAACTGCCTTTCCAAAATGTTGTCGACTACACCCAGATATCAATTAAATGGCCATCTACTTTAATAGGTCCCGAGCTTTTGCAGTACCTAGAGTCAATACCAGATAAAAATATAGAGGGGATGATAAGCCGGGGCAGACAAGTACGATGTTTATGGGCGTATGCTTCAGAATCAGCTTCGTGTTCGGCAATGCATGGGATTCTTTGGGAGCTTCAGAGGAAAGTGAGGCTGTTTCACCAGTCGACGGAAACATTCTGGTTGCACAATGGATCTGTTGTAAATAGAAACTTGGTAGAATTTTCTGATTGGAGACCGCCGATGCCTTTGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exostosin NAM

Protein Analysis

462

Amino Acids

52.56

Weight (kDa)

9.48

Isoelectric Point (pI)

42.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exostosin_GT47 PF03016 87 - 370 7e-41 Exostosin GT47 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 629
AarI CACCTGC 1 cut(s) 951
Acc16I TGCGCA 1 cut(s) 947
Acc36I ACCTGC 1 cut(s) 951
AccBSI CCGCTC 1 cut(s) 135
AccI GTMKAC 3 cut(s) 276, 1077, 1295
AccII CGCG 3 cut(s) 38, 288, 324
AccIII TCCGGA 1 cut(s) 411
AciI CCGC 8 cut(s) 67, 102, 133, 142, 165, 213, 286, 1367
AclWI GGATC 1 cut(s) 1330
AcoI YGGCCR 4 cut(s) 214, 559, 832, 1103
AcsI RAATTY 3 cut(s) 400, 863, 1349
AcuI CTGAAG 2 cut(s) 1203, 1250
AcvI CACGTG 1 cut(s) 974
AfaI GTAC 3 cut(s) 245, 1139, 1197
AfiI CCNNNNNNNGG 7 cut(s) 94, 189, 239, 240, 975, 976, 1297
AflII CTTAAG 1 cut(s) 839
AgsI TTSAA 4 cut(s) 355, 858, 895, 938
AloI GAACNNNNNNTCC 2 cut(s) 1047, 1079
AluBI AGCT 6 cut(s) 195, 599, 853, 1129, 1229, 1264
AluI AGCT 6 cut(s) 195, 599, 853, 1129, 1229, 1264
Alw21I GWGCWC 3 cut(s) 197, 601, 820
Alw26I GTCTC 2 cut(s) 128, 1357
Alw44I GTGCAC 1 cut(s) 816
AlwI GGATC 1 cut(s) 1330
Ama87I CYCGRG 1 cut(s) 1124
Aor13HI TCCGGA 1 cut(s) 411
ApaI GGGCCC 1 cut(s) 756
ApaLI GTGCAC 1 cut(s) 816
ApeKI GCWGC 2 cut(s) 40, 52
ApoI RAATTY 3 cut(s) 400, 863, 1349
ArsI GACNNNNNNTTYG 4 cut(s) 428, 460, 557, 589
AspLEI GCGC 4 cut(s) 38, 40, 152, 948
AspS9I GGNCC 7 cut(s) 231, 248, 752, 753, 763, 905, 1121
AsuC2I CCSGG 2 cut(s) 720, 1184
AsuHPI GGTGA 4 cut(s) 109, 199, 671, 1280
AvaI CYCGRG 1 cut(s) 1124
AvaII GGWCC 2 cut(s) 763, 1121
AxyI CCTNAGG 1 cut(s) 654
BaeGI GKGCMC 3 cut(s) 371, 756, 820
BalI TGGCCA 1 cut(s) 1105
BanII GRGCYC 4 cut(s) 88, 197, 601, 756
BbrPI CACGTG 1 cut(s) 974
Bbv12I GWGCWC 3 cut(s) 197, 601, 820
BbvI GCAGC 2 cut(s) 52, 64
BccI CCATC 3 cut(s) 320, 472, 1114
BceAI ACGGC 6 cut(s) 152, 262, 313, 546, 798, 819
BcgI CGANNNNNNTGC 1 cut(s) 1360
BcnI CCSGG 2 cut(s) 720, 1184
BcoDI GTCTC 2 cut(s) 128, 1357
BfaI CTAG 1 cut(s) 1142
BfrI CTTAAG 1 cut(s) 839
BfuAI ACCTGC 1 cut(s) 951
BglI GCCNNNNNGGC 1 cut(s) 366
BglII AGATCT 4 cut(s) 172, 220, 267, 611
BisI GCNGC 4 cut(s) 41, 53, 102, 214
BlsI GCNGC 4 cut(s) 42, 54, 103, 215
Bme1390I CCNGG 2 cut(s) 720, 1184
Bme18I GGWCC 2 cut(s) 763, 1121
BmeT110I CYCGRG 1 cut(s) 1124
BmgT120I GGNCC 7 cut(s) 231, 248, 752, 753, 763, 905, 1121
BmiI GGNNCC 5 cut(s) 85, 233, 250, 754, 1123
BmrFI CCNGG 2 cut(s) 720, 1184
BmsI GCATC 3 cut(s) 271, 303, 1362
BplI GAGNNNNNCTC 2 cut(s) 75, 107
BpmI CTGGAG 1 cut(s) 615
BpuEI CTTGAG 2 cut(s) 248, 287
BpuMI CCSGG 2 cut(s) 720, 1184
BsaAI YACGTR 1 cut(s) 974
BsaI GGTCTC 1 cut(s) 1357
BsaJI CCNNGG 3 cut(s) 342, 759, 1183
BsaWI WCCGGW 1 cut(s) 411
Bsc4I CCNNNNNNNGG 7 cut(s) 94, 189, 239, 240, 975, 976, 1297
Bse118I RCCGGY 1 cut(s) 209
Bse1I ACTGG 3 cut(s) 120, 361, 1291
Bse21I CCTNAGG 1 cut(s) 654
Bse3DI GCAATG 2 cut(s) 955, 1248
BseAI TCCGGA 1 cut(s) 411
BseDI CCNNGG 3 cut(s) 342, 759, 1183
BseGI GGATG 2 cut(s) 480, 1179
BseLI CCNNNNNNNGG 7 cut(s) 94, 189, 239, 240, 975, 976, 1297
BseMI GCAATG 2 cut(s) 955, 1248
BseMII CTCAG 1 cut(s) 645
BseNI ACTGG 3 cut(s) 120, 361, 1291
BsePI GCGCGC 1 cut(s) 36
BseRI GAGGAG 2 cut(s) 80, 167
BseSI GKGCMC 3 cut(s) 371, 756, 820
BseX3I CGGCCG 1 cut(s) 214
BseXI GCAGC 2 cut(s) 52, 64
BsgI GTGCAG 1 cut(s) 46
Bsh1236I CGCG 3 cut(s) 38, 288, 324
Bsh1285I CGRYCG 1 cut(s) 217
BsiEI CGRYCG 1 cut(s) 217
BsiHKAI GWGCWC 3 cut(s) 197, 601, 820
BsiHKCI CYCGRG 1 cut(s) 1124
BsiSI CCGG 5 cut(s) 210, 412, 539, 720, 1183
BslFI GGGAC 2 cut(s) 1013, 1107
BslI CCNNNNNNNGG 7 cut(s) 94, 189, 239, 240, 975, 976, 1297
BsmAI GTCTC 2 cut(s) 128, 1357
BsmFI GGGAC 2 cut(s) 1013, 1107
Bso31I GGTCTC 1 cut(s) 1357
BsoBI CYCGRG 1 cut(s) 1124
Bsp120I GGGCCC 1 cut(s) 752
Bsp1286I GDGCHC 6 cut(s) 88, 197, 371, 601, 756, 820
Bsp13I TCCGGA 1 cut(s) 411
Bsp68I TCGCGA 1 cut(s) 324
BspACI CCGC 8 cut(s) 67, 102, 133, 142, 165, 213, 286, 1367
BspCNI CTCAG 1 cut(s) 646
BspEI TCCGGA 1 cut(s) 411
BspFNI CGCG 3 cut(s) 38, 288, 324
BspLI GGNNCC 5 cut(s) 85, 233, 250, 754, 1123
BspMI ACCTGC 1 cut(s) 951
BspPI GGATC 1 cut(s) 1330
BspTI CTTAAG 1 cut(s) 839
BspTNI GGTCTC 1 cut(s) 1357
BsrBI CCGCTC 1 cut(s) 135
BsrDI GCAATG 2 cut(s) 955, 1248
BsrFI RCCGGY 1 cut(s) 209
BsrI ACTGG 3 cut(s) 120, 361, 1291
BssAI RCCGGY 1 cut(s) 209
BssECI CCNNGG 3 cut(s) 342, 759, 1183
BssHII GCGCGC 1 cut(s) 36
BssT1I CCWWGG 2 cut(s) 342, 759
Bst4CI ACNGT 1 cut(s) 737
Bst6I CTCTTC 1 cut(s) 999
BstAFI CTTAAG 1 cut(s) 839
BstBAI YACGTR 1 cut(s) 974
BstC8I GCNNGC 6 cut(s) 38, 71, 286, 349, 371, 832
BstDEI CTNAG 2 cut(s) 127, 654
BstF5I GGATG 2 cut(s) 480, 1179
BstFNI CGCG 3 cut(s) 38, 288, 324
BstHHI GCGC 4 cut(s) 38, 40, 152, 948
BstMAI GTCTC 2 cut(s) 128, 1357
BstMCI CGRYCG 1 cut(s) 217
BstMWI GCNNNNNNNGC 4 cut(s) 49, 141, 357, 366
BstSCI CCNGG 2 cut(s) 718, 1182
BstSLI GKGCMC 3 cut(s) 371, 756, 820
BstUI CGCG 3 cut(s) 38, 288, 324
BstV1I GCAGC 2 cut(s) 52, 64
BstX2I RGATCY 5 cut(s) 172, 220, 267, 611, 1322
BstYI RGATCY 5 cut(s) 172, 220, 267, 611, 1322
BstZI CGGCCG 1 cut(s) 214
Bsu36I CCTNAGG 1 cut(s) 654
BtgZI GCGATG 1 cut(s) 339
BtsCI GGATG 2 cut(s) 480, 1179
BtsIMutI CAGTG 2 cut(s) 368, 907
BtuMI TCGCGA 1 cut(s) 324
BveI ACCTGC 1 cut(s) 951
Cac8I GCNNGC 6 cut(s) 38, 71, 286, 349, 371, 832
CfoI GCGC 4 cut(s) 38, 40, 152, 948
Cfr10I RCCGGY 1 cut(s) 209
Cfr13I GGNCC 7 cut(s) 231, 248, 752, 753, 763, 905, 1121
Csp6I GTAC 3 cut(s) 244, 1138, 1196
CviAII CATG 3 cut(s) 699, 987, 1247
CviQI GTAC 3 cut(s) 244, 1138, 1196
DdeI CTNAG 2 cut(s) 127, 654
EaeI YGGCCR 4 cut(s) 214, 559, 832, 1103
EagI CGGCCG 1 cut(s) 214
Eam1104I CTCTTC 1 cut(s) 999
EarI CTCTTC 1 cut(s) 999
EciI GGCGGA 1 cut(s) 131
Ecl136II GAGCTC 2 cut(s) 195, 599
EclXI CGGCCG 1 cut(s) 214
Eco130I CCWWGG 2 cut(s) 342, 759
Eco24I GRGCYC 4 cut(s) 88, 197, 601, 756
Eco31I GGTCTC 1 cut(s) 1357
Eco32I GATATC 2 cut(s) 709, 1092
Eco47I GGWCC 2 cut(s) 763, 1121
Eco52I CGGCCG 1 cut(s) 214
Eco53kI GAGCTC 2 cut(s) 195, 599
Eco57I CTGAAG 2 cut(s) 1203, 1250
Eco72I CACGTG 1 cut(s) 974
Eco81I CCTNAGG 1 cut(s) 654
Eco88I CYCGRG 1 cut(s) 1124
EcoICRI GAGCTC 2 cut(s) 195, 599
EcoO109I RGGNCCY 1 cut(s) 1121
EcoRV GATATC 2 cut(s) 709, 1092
EcoT14I CCWWGG 2 cut(s) 342, 759
EcoT22I ATGCAT 1 cut(s) 1248
EcoT38I GRGCYC 4 cut(s) 88, 197, 601, 756
ErhI CCWWGG 2 cut(s) 342, 759
FaeI CATG 3 cut(s) 702, 990, 1250
FalI AAGNNNNNCTT 2 cut(s) 335, 367
FaqI GGGAC 2 cut(s) 1013, 1107
FatI CATG 3 cut(s) 698, 986, 1246
FauI CCCGC 3 cut(s) 74, 172, 293
FauNDI CATATG 1 cut(s) 514
FblI GTMKAC 3 cut(s) 276, 1077, 1295
Fnu4HI GCNGC 4 cut(s) 41, 53, 102, 214
FokI GGATG 2 cut(s) 487, 1186
FriOI GRGCYC 4 cut(s) 88, 197, 601, 756
Fsp4HI GCNGC 4 cut(s) 41, 53, 102, 214
FspBI CTAG 1 cut(s) 1142
FspI TGCGCA 1 cut(s) 947
GlaI GCGC 4 cut(s) 37, 39, 151, 947
GluI GCNGC 4 cut(s) 41, 53, 102, 214
GsuI CTGGAG 1 cut(s) 615
HapII CCGG 5 cut(s) 210, 412, 539, 720, 1183
HhaI GCGC 4 cut(s) 38, 40, 152, 948
Hin1II CATG 3 cut(s) 702, 990, 1250
Hin6I GCGC 4 cut(s) 36, 38, 150, 946
HinP1I GCGC 4 cut(s) 36, 38, 150, 946
HincII GTYRAC 2 cut(s) 1078, 1296
HindII GTYRAC 2 cut(s) 1078, 1296
HinfI GANTC 6 cut(s) 408, 980, 991, 1145, 1223, 1252
HpaII CCGG 5 cut(s) 210, 412, 539, 720, 1183
HphI GGTGA 4 cut(s) 109, 199, 671, 1280
Hpy166II GTNNAC 5 cut(s) 277, 766, 818, 1078, 1296
Hpy188I TCNGA 7 cut(s) 83, 474, 666, 1045, 1222, 1269, 1357
Hpy188III TCNNGA 7 cut(s) 265, 323, 405, 412, 869, 887, 1124
Hpy8I GTNNAC 5 cut(s) 277, 766, 818, 1078, 1296
Hpy99I CGWCG 2 cut(s) 299, 1300
HpyAV CCTTC 4 cut(s) 118, 148, 514, 915
HpyCH4III ACNGT 1 cut(s) 737
HpyCH4IV ACGT 2 cut(s) 273, 973
HpyCH4V TGCA 5 cut(s) 63, 818, 1135, 1246, 1315
HpyF10VI GCNNNNNNNGC 4 cut(s) 49, 141, 357, 366
HpyF3I CTNAG 2 cut(s) 127, 654
HpySE526I ACGT 2 cut(s) 273, 973
Hsp92II CATG 3 cut(s) 702, 990, 1250
HspAI GCGC 4 cut(s) 36, 38, 150, 946
Kpn2I TCCGGA 1 cut(s) 411
LmnI GCTCC 6 cut(s) 83, 140, 192, 200, 596, 1261
Lsp1109I GCAGC 2 cut(s) 52, 64
LweI GCATC 3 cut(s) 271, 303, 1362
MaeI CTAG 1 cut(s) 1142
MaeII ACGT 2 cut(s) 273, 973
MaeIII GTNAC 2 cut(s) 205, 563
MbiI CCGCTC 1 cut(s) 135
MboII GAAGA 4 cut(s) 103, 106, 433, 986
MflI RGATCY 5 cut(s) 172, 220, 267, 611, 1322
MhlI GDGCHC 6 cut(s) 88, 197, 371, 601, 756, 820
MlsI TGGCCA 1 cut(s) 1105
MluCI AATT 7 cut(s) 184, 400, 714, 863, 914, 1095, 1349
MluNI TGGCCA 1 cut(s) 1105
MlyI GAGTC 3 cut(s) 985, 989, 1154
MmeI TCCRAC 1 cut(s) 858
Mox20I TGGCCA 1 cut(s) 1105
Mph1103I ATGCAT 1 cut(s) 1248
MroI TCCGGA 1 cut(s) 411
MscI TGGCCA 1 cut(s) 1105
MseI TTAA 8 cut(s) 378, 489, 504, 527, 693, 840, 1098, 1115
Msp20I TGGCCA 1 cut(s) 1105
MspCI CTTAAG 1 cut(s) 839
MspI CCGG 5 cut(s) 210, 412, 539, 720, 1183
MspR9I CCNGG 2 cut(s) 720, 1184
MvnI CGCG 3 cut(s) 38, 288, 324
MwoI GCNNNNNNNGC 4 cut(s) 49, 141, 357, 366
NciI CCSGG 2 cut(s) 720, 1184
NdeI CATATG 1 cut(s) 514
NlaIII CATG 3 cut(s) 702, 990, 1250
NlaIV GGNNCC 5 cut(s) 85, 233, 250, 754, 1123
NmeAIII GCCGAG 1 cut(s) 242
NmuCI GTSAC 2 cut(s) 205, 563
NruI TCGCGA 1 cut(s) 324
NsbI TGCGCA 1 cut(s) 947
NsiI ATGCAT 1 cut(s) 1248
PaqCI CACCTGC 1 cut(s) 951
PauI GCGCGC 1 cut(s) 36
PcsI WCGNNNNNNNCGW 1 cut(s) 285
PfeI GAWTC 3 cut(s) 408, 1223, 1252
PfoI TCCNGGA 1 cut(s) 718
PkrI GCNGC 4 cut(s) 42, 54, 103, 215
PleI GAGTC 3 cut(s) 985, 988, 1153
PmaCI CACGTG 1 cut(s) 974
PmlI CACGTG 1 cut(s) 974
PpsI GAGTC 3 cut(s) 985, 988, 1153
Ppu21I YACGTR 1 cut(s) 974
PpuMI RGGWCCY 1 cut(s) 1121
PsiI TTATAA 1 cut(s) 629
Psp124BI GAGCTC 2 cut(s) 197, 601
Psp5II RGGWCCY 1 cut(s) 1121
PspCI CACGTG 1 cut(s) 974
PspN4I GGNNCC 5 cut(s) 85, 233, 250, 754, 1123
PspOMI GGGCCC 1 cut(s) 752
PspPI GGNCC 7 cut(s) 231, 248, 752, 753, 763, 905, 1121
PspPPI RGGWCCY 1 cut(s) 1121
PsuI RGATCY 5 cut(s) 172, 220, 267, 611, 1322
PteI GCGCGC 1 cut(s) 36
RruI TCGCGA 1 cut(s) 324
RsaI GTAC 3 cut(s) 245, 1139, 1197
RsaNI GTAC 3 cut(s) 244, 1138, 1196
SacI GAGCTC 2 cut(s) 197, 601
SalI GTCGAC 2 cut(s) 1076, 1294
SaqAI TTAA 8 cut(s) 378, 489, 504, 527, 693, 840, 1098, 1115
SatI GCNGC 4 cut(s) 41, 53, 102, 214
Sau96I GGNCC 7 cut(s) 231, 248, 752, 753, 763, 905, 1121
SchI GAGTC 3 cut(s) 985, 989, 1154
ScrFI CCNGG 2 cut(s) 720, 1184
SduI GDGCHC 6 cut(s) 88, 197, 371, 601, 756, 820
SfaNI GCATC 3 cut(s) 271, 303, 1362
SgrAI CRCCGGYG 1 cut(s) 209
SinI GGWCC 2 cut(s) 763, 1121
SmlI CTYRAG 3 cut(s) 263, 302, 839
SmoI CTYRAG 3 cut(s) 263, 302, 839
Sse9I AATT 7 cut(s) 184, 400, 714, 863, 914, 1095, 1349
SsiI CCGC 8 cut(s) 67, 102, 133, 142, 165, 213, 286, 1367
SspMI CTAG 1 cut(s) 1142
SstI GAGCTC 2 cut(s) 197, 601
StyD4I CCNGG 2 cut(s) 718, 1182
StyI CCWWGG 2 cut(s) 342, 759
TaaI ACNGT 1 cut(s) 737
TaiI ACGT 2 cut(s) 276, 976
TaqI TCGA 7 cut(s) 258, 294, 336, 404, 997, 1077, 1295
TasI AATT 7 cut(s) 184, 400, 714, 863, 914, 1095, 1349
TauI GCSGC 2 cut(s) 104, 216
TfiI GAWTC 3 cut(s) 408, 1223, 1252
Tru1I TTAA 8 cut(s) 378, 489, 504, 527, 693, 840, 1098, 1115
Tru9I TTAA 8 cut(s) 378, 489, 504, 527, 693, 840, 1098, 1115
TscAI CASTG 2 cut(s) 368, 907
TseFI GTSAC 2 cut(s) 205, 563
TseI GCWGC 2 cut(s) 40, 52
Tsp45I GTSAC 2 cut(s) 205, 563
TspDTI ATGAA 1 cut(s) 444
TspGWI ACGGA 3 cut(s) 187, 205, 1313
TspRI CASTG 2 cut(s) 368, 907
Vha464I CTTAAG 1 cut(s) 839
VneI GTGCAC 1 cut(s) 816
VpaK11BI GGWCC 2 cut(s) 763, 1121
XapI RAATTY 3 cut(s) 400, 863, 1349
XmiI GTMKAC 3 cut(s) 276, 1077, 1295
XspI CTAG 1 cut(s) 1142
Zsp2I ATGCAT 1 cut(s) 1248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.