Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0006299

Physical Location & Seq

Genomic Coordinates Forward (+)

2945452 .. 2958246

Gene Structure 12795 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1016800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1461 bp

ATGGGATGGAGGGGCATTTTATGTTCTTCCTTTTTCTTTATATCCCTAGTCGAGTCCTCTAGCAGATTAGGAGAAATGTGGTCACTAAACCTTGGTGCTGAATCATCAGCAGCTTTCAGCTGGCCAGTCCTCAGTGCTAGCATTTTCGTACTTGTTGCTCTTCTTCTCTCCATGTACCTTATCTTTGAGCATTTAGCTGCTTATAATCAGCCAGAGGAGCAGAAGTTTCTGATTGGTCTCATTTTGATGGTTCCTGTCTATGCACTGGAATCGTTTTTGTCACTGTTGGACTCCAATGCGGCCTTCAATTGCGAAGCAATTCGGGACTGCTATGAGGCTTTTGCACTATATTGTTTTGAGAGATACCTGATAGCGTGCTTAGGTGGCGAGGAGAGGACAATTGAATTTATGGAGAGTCAAAGCGTAGTCGACTCGAGCACACCTCTTTTGAAAGAATCTTATGCTTACGGAGTTGTAGAGCATCCTTTCCCACTGAATTGCCTCATAGGAGACTGGCCTCTTGGCTCCGCATTCTACTATGCTGTTAAAATTGGCATCGTTCAGTATATGATACTGAAATTGATCTGCGCTTTGCTAGCAATGATTCTTGAGTCTTTTGGTGTTTATGGAGAGGGGAAATTTGATGGGAGATATGGCTATCCTTACTTGGCAGTTGTTCTAAATTTTAGTCAGACATGGGCCCTATACTGCCTTGTGCAGTTTTACTCTGTTATTAAAGACAAATTACAACCCATTAAACCCTTGGCAAAGTTTCTGACTTTTAAGTCCATTGTGTTCTTGACATGGTGGCAAGGAATCGCTGTTGCATTTCTTTTCTCTATGGGTGCCTTCAAAGGGTCATTGGCTCAGGAGCTGAAAACTCGGATACAAGACTACATCATATGCATAGAGATGGGTGTTGCTGCTGTAGTCCACCTTTATGTGTTTCCAGCTGTACCTTACAAACGTGGAGAAAGATGTGTTCGTAATGTTGCTGTAATGACTGACTATGCATCTCTGGGAGCACCTCCCGATCCAGAAGAGGTACGTGATTGTGAAAGAACAACCAGAGTGCATTTGGCAGTGGCACGGCACGATGAGAGAGAAAAGCGCCTGAATTTCCCCCAGAGTGTTCGTGATGTGATTTTCGGAAGTGGAGAAATTATGGTGGATGACATGAAGTATACAGTTTCTCATGTGGTAGAACCAGTTGAAAGAGGGATTGCAAAGATAAACAAAACCTTGCATCAGATATCCGAGAATGTGAAACGCCACGAGGAGCAGAGGAAGAGCATCAAGGACGATAGTTATCTGATCCCCTTGAATTCATGGAATAGAGAGTTTTCCGATGCTCACGACAATCTTGTTGGAGGCAGTGCCAGTGACAGTGGCATAACCAGTGGTAGGAGGCAGCAGTCCCAGTCTAAAGCTTCAACATCTCGGACTAGAGCGTGGAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006810 GO:0006996 GO:0007033 GO:0008150 GO:0009705 GO:0009966 GO:0009968 GO:0009987 GO:0010646 GO:0010648 GO:0016020 GO:0016021 GO:0016043 GO:0016192 GO:0023051 GO:0023057 GO:0031090 GO:0031224 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0051179 GO:0051234 GO:0065007 GO:0071840 GO:0098588 GO:0098805 GO:0098876 GO:1900457 GO:1900458

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Solute_trans_a

Protein Analysis

487

Amino Acids

54.9

Weight (kDa)

6.05

Isoelectric Point (pI)

40.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Solute_trans_a	PF03619	44 - 321	1.1e-90	Organic solute transporter Ostalpha

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006299)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G77220
fragaria_vesca	FvH4_2g14580 FvH4_2g14580 FvH4_2g14580 FvH4_2g14580 FvH4_2g14580 FvH4_2g14580 FvH4_2g14580 FvH4_2g14580
malus_domestica	MD05G1016800.v1.1 MD10G1016600.v1.1
prunus_persica	Prupe.8G020400_v2.0.a1 Prupe.8G020400_v2.0.a1 Prupe.8G020400_v2.0.a1
pyrus_communis	pycom05g00950 pycom10g01090
rosa_chinensis	RchiOBHm_Chr6g0277041
rosa_laevigata	RLG00000013343
rosa_multiflora	Rmu_sc0000206.1_g000009
rosa_roxburghii	Rroxscaffold_7G00191750
rosa_rugosa	Rorug06G0105600 Rorug06G0105700
rosa_samantha	Rh6AG215700 Rh6BG220400 Rh6CG223200 Rh6DG212800
rosa_wichuraiana	Rw6G018830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	204
AasI	GACNNNNNNGTC	1 cut(s)	784
AccB1I	GGYRCC	1 cut(s)	845
AccI	GTMKAC	2 cut(s)	429, 1184
AciI	CCGC	2 cut(s)	299, 528
AclWI	GGATC	2 cut(s)	1028, 1309
AcoI	YGGCCR	1 cut(s)	122
AcsI	RAATTY	5 cut(s)	404, 638, 682, 1117, 1324
AfaI	GTAC	4 cut(s)	150, 176, 957, 1047
AfiI	CCNNNNNNNGG	2 cut(s)	855, 1404
AgsI	TTSAA	7 cut(s)	307, 404, 451, 853, 1214, 1324, 1434
AjuI	GAANNNNNNNTTGG	2 cut(s)	287, 319
AluBI	AGCT	6 cut(s)	113, 120, 197, 874, 953, 1430
AluI	AGCT	6 cut(s)	113, 120, 197, 874, 953, 1430
Alw21I	GWGCWC	2 cut(s)	440, 1027
Alw26I	GTCTC	2 cut(s)	242, 504
AlwI	GGATC	2 cut(s)	1028, 1309
AlwNI	CAGNNNCTG	1 cut(s)	874
Ama87I	CYCGRG	1 cut(s)	433
AoxI	GGCC	4 cut(s)	122, 300, 515, 699
ApaI	GGGCCC	1 cut(s)	703
ApeKI	GCWGC	4 cut(s)	110, 197, 923, 1411
ApoI	RAATTY	5 cut(s)	404, 638, 682, 1117, 1324
Asp700I	GAANNNNTTC	1 cut(s)	318
AspLEI	GCGC	2 cut(s)	590, 1113
AspS9I	GGNCC	2 cut(s)	699, 700
AsuNHI	GCTAGC	2 cut(s)	137, 595
AvaI	CYCGRG	1 cut(s)	433
BaeGI	GKGCMC	1 cut(s)	703
BalI	TGGCCA	1 cut(s)	124
BanI	GGYRCC	1 cut(s)	845
BanII	GRGCYC	1 cut(s)	703
BauI	CACGAG	1 cut(s)	1274
Bbv12I	GWGCWC	2 cut(s)	440, 1027
BbvI	GCAGC	4 cut(s)	122, 184, 910, 1423
BccI	CCATC	3 cut(s)	241, 638, 907
BceAI	ACGGC	1 cut(s)	1106
BcgI	CGANNNNNNTGC	2 cut(s)	252, 286
BciVI	GTATCC	1 cut(s)	879
BcoDI	GTCTC	2 cut(s)	242, 504
BfaI	CTAG	5 cut(s)	47, 60, 138, 596, 1446
BfmI	CTRYAG	1 cut(s)	927
BfoI	RGCGCY	1 cut(s)	1114
BfuI	GTATCC	1 cut(s)	879
BisI	GCNGC	5 cut(s)	111, 198, 300, 924, 1412
BlsI	GCNGC	5 cut(s)	112, 199, 301, 925, 1413
BmeT110I	CYCGRG	1 cut(s)	433
BmgT120I	GGNCC	2 cut(s)	699, 700
BmiI	GGNNCC	4 cut(s)	252, 526, 701, 847
BmrI	ACTGGG	1 cut(s)	1414
BmsI	GCATC	6 cut(s)	490, 564, 1022, 1255, 1302, 1339
BmtI	GCTAGC	2 cut(s)	141, 599
BmuI	ACTGGG	1 cut(s)	1414
BplI	GAGNNNNNCTC	2 cut(s)	427, 459
Bpu10I	CCTNAGC	2 cut(s)	379, 867
BpuEI	CTTGAG	1 cut(s)	629
BsaAI	YACGTR	1 cut(s)	1049
BsaBI	GATNNNNATC	1 cut(s)	1308
BsaI	GGTCTC	1 cut(s)	242
BsaJI	CCNNGG	2 cut(s)	91, 762
BsaXI	ACNNNNNCTCC	2 cut(s)	209, 239
Bsc4I	CCNNNNNNNGG	2 cut(s)	855, 1404
Bse1I	ACTGG	7 cut(s)	125, 270, 518, 1208, 1380, 1398, 1420
Bse3DI	GCAATG	1 cut(s)	606
Bse8I	GATNNNNATC	1 cut(s)	1308
BseDI	CCNNGG	2 cut(s)	91, 762
BseGI	GGATG	3 cut(s)	11, 481, 1177
BseJI	GATNNNNATC	1 cut(s)	1308
BseLI	CCNNNNNNNGG	2 cut(s)	855, 1404
BseMI	GCAATG	1 cut(s)	606
BseMII	CTCAG	2 cut(s)	145, 881
BseNI	ACTGG	7 cut(s)	125, 270, 518, 1208, 1380, 1398, 1420
BseRI	GAGGAG	3 cut(s)	230, 404, 1292
BseSI	GKGCMC	1 cut(s)	703
BseXI	GCAGC	4 cut(s)	122, 184, 910, 1423
BsgI	GTGCAG	1 cut(s)	737
BshFI	GGCC	4 cut(s)	124, 302, 517, 701
BshNI	GGYRCC	1 cut(s)	845
BsiHKAI	GWGCWC	2 cut(s)	440, 1027
BsiHKCI	CYCGRG	1 cut(s)	433
BslFI	GGGAC	2 cut(s)	338, 1402
BslI	CCNNNNNNNGG	2 cut(s)	855, 1404
BsmAI	GTCTC	2 cut(s)	242, 504
BsmFI	GGGAC	2 cut(s)	338, 1402
BsmI	GAATGC	1 cut(s)	530
BsnI	GGCC	4 cut(s)	124, 302, 517, 701
Bso31I	GGTCTC	1 cut(s)	242
BsoBI	CYCGRG	1 cut(s)	433
Bsp120I	GGGCCC	1 cut(s)	699
Bsp1286I	GDGCHC	3 cut(s)	440, 703, 1027
Bsp143I	GATC	3 cut(s)	582, 1033, 1314
BspACI	CCGC	2 cut(s)	299, 528
BspANI	GGCC	4 cut(s)	124, 302, 517, 701
BspCNI	CTCAG	2 cut(s)	144, 880
BspLI	GGNNCC	4 cut(s)	252, 526, 701, 847
BspOI	GCTAGC	2 cut(s)	141, 599
BspPI	GGATC	2 cut(s)	1028, 1309
BspQI	GCTCTTC	2 cut(s)	165, 1283
BspT107I	GGYRCC	1 cut(s)	845
BspTNI	GGTCTC	1 cut(s)	242
BsrDI	GCAATG	1 cut(s)	606
BsrI	ACTGG	7 cut(s)	125, 270, 518, 1208, 1380, 1398, 1420
BssECI	CCNNGG	2 cut(s)	91, 762
BssMI	GATC	3 cut(s)	582, 1033, 1314
BssNAI	GTATAC	1 cut(s)	1185
BssSI	CACGAG	1 cut(s)	1274
BssT1I	CCWWGG	2 cut(s)	91, 762
Bst1107I	GTATAC	1 cut(s)	1185
Bst2BI	CACGAG	1 cut(s)	1274
Bst4CI	ACNGT	3 cut(s)	285, 1189, 1388
Bst6I	CTCTTC	3 cut(s)	165, 1035, 1283
BstBAI	YACGTR	1 cut(s)	1049
BstC8I	GCNNGC	4 cut(s)	122, 139, 376, 597
BstDEI	CTNAG	3 cut(s)	131, 379, 867
BstF5I	GGATG	3 cut(s)	11, 481, 1177
BstH2I	RGCGCY	1 cut(s)	1114
BstHHI	GCGC	2 cut(s)	590, 1113
BstKTI	GATC	3 cut(s)	585, 1036, 1317
BstMAI	GTCTC	2 cut(s)	242, 504
BstMBI	GATC	3 cut(s)	582, 1033, 1314
BstMWI	GCNNNNNNNGC	3 cut(s)	217, 384, 596
BstSFI	CTRYAG	1 cut(s)	927
BstSLI	GKGCMC	1 cut(s)	703
BstV1I	GCAGC	4 cut(s)	122, 184, 910, 1423
BstZ17I	GTATAC	1 cut(s)	1185
BsuI	GTATCC	1 cut(s)	879
BsuRI	GGCC	4 cut(s)	124, 302, 517, 701
BtsCI	GGATG	3 cut(s)	11, 481, 1177
BtsI	GCAGTG	2 cut(s)	1089, 1381
BtsIMutI	CAGTG	9 cut(s)	139, 263, 281, 491, 1089, 1381, 1387, 1393, 1405
Cac8I	GCNNGC	4 cut(s)	122, 139, 376, 597
CaiI	CAGNNNCTG	1 cut(s)	874
CfoI	GCGC	2 cut(s)	590, 1113
Cfr13I	GGNCC	2 cut(s)	699, 700
Csp6I	GTAC	4 cut(s)	149, 175, 956, 1046
CviAII	CATG	6 cut(s)	172, 696, 804, 1177, 1196, 1329
CviQI	GTAC	4 cut(s)	149, 175, 956, 1046
DdeI	CTNAG	3 cut(s)	131, 379, 867
DpnI	GATC	3 cut(s)	584, 1035, 1316
DpnII	GATC	3 cut(s)	582, 1033, 1314
DrdI	GACNNNNNNGTC	1 cut(s)	784
DseDI	GACNNNNNNGTC	1 cut(s)	784
EaeI	YGGCCR	1 cut(s)	122
Eam1104I	CTCTTC	3 cut(s)	165, 1035, 1283
EarI	CTCTTC	3 cut(s)	165, 1035, 1283
Eco130I	CCWWGG	2 cut(s)	91, 762
Eco24I	GRGCYC	1 cut(s)	703
Eco31I	GGTCTC	1 cut(s)	242
Eco32I	GATATC	1 cut(s)	1254
Eco88I	CYCGRG	1 cut(s)	433
EcoO109I	RGGNCCY	1 cut(s)	700
EcoRI	GAATTC	1 cut(s)	1324
EcoRV	GATATC	1 cut(s)	1254
EcoT14I	CCWWGG	2 cut(s)	91, 762
EcoT22I	ATGCAT	2 cut(s)	908, 1015
EcoT38I	GRGCYC	1 cut(s)	703
ErhI	CCWWGG	2 cut(s)	91, 762
FaeI	CATG	6 cut(s)	175, 699, 807, 1180, 1199, 1332
FaqI	GGGAC	2 cut(s)	338, 1402
FatI	CATG	6 cut(s)	171, 695, 803, 1176, 1195, 1328
FauNDI	CATATG	1 cut(s)	902
FblI	GTMKAC	2 cut(s)	429, 1184
Fnu4HI	GCNGC	5 cut(s)	111, 198, 300, 924, 1412
FokI	GGATG	3 cut(s)	18, 468, 1184
FriOI	GRGCYC	1 cut(s)	703
Fsp4HI	GCNGC	5 cut(s)	111, 198, 300, 924, 1412
FspBI	CTAG	5 cut(s)	47, 60, 138, 596, 1446
GlaI	GCGC	2 cut(s)	589, 1112
GluI	GCNGC	5 cut(s)	111, 198, 300, 924, 1412
HaeII	RGCGCY	1 cut(s)	1114
HaeIII	GGCC	4 cut(s)	124, 302, 517, 701
HhaI	GCGC	2 cut(s)	590, 1113
Hin1II	CATG	6 cut(s)	175, 699, 807, 1180, 1199, 1332
Hin6I	GCGC	2 cut(s)	588, 1111
HinP1I	GCGC	2 cut(s)	588, 1111
HincII	GTYRAC	1 cut(s)	430
HindII	GTYRAC	1 cut(s)	430
HindIII	AAGCTT	1 cut(s)	1428
Hpy166II	GTNNAC	3 cut(s)	430, 934, 1185
Hpy188III	TCNNGA	8 cut(s)	323, 608, 799, 869, 1031, 1037, 1136, 1355
Hpy8I	GTNNAC	3 cut(s)	430, 934, 1185
HpyAV	CCTTC	2 cut(s)	313, 859
HpyCH4III	ACNGT	3 cut(s)	285, 1189, 1388
HpyCH4IV	ACGT	2 cut(s)	967, 1048
HpyCH4V	TGCA	9 cut(s)	263, 344, 718, 827, 906, 1013, 1075, 1226, 1246
HpyF10VI	GCNNNNNNNGC	3 cut(s)	217, 384, 596
HpyF3I	CTNAG	3 cut(s)	131, 379, 867
HpySE526I	ACGT	2 cut(s)	967, 1048
Hsp92II	CATG	6 cut(s)	175, 699, 807, 1180, 1199, 1332
HspAI	GCGC	2 cut(s)	588, 1111
Kzo9I	GATC	3 cut(s)	582, 1033, 1314
LguI	GCTCTTC	2 cut(s)	165, 1283
LmnI	GCTCC	5 cut(s)	217, 530, 871, 1022, 1279
Lsp1109I	GCAGC	4 cut(s)	122, 184, 910, 1423
LweI	GCATC	6 cut(s)	490, 564, 1022, 1255, 1302, 1339
MaeI	CTAG	5 cut(s)	47, 60, 138, 596, 1446
MaeII	ACGT	2 cut(s)	967, 1048
MaeIII	GTNAC	3 cut(s)	81, 279, 1382
MalI	GATC	3 cut(s)	584, 1035, 1316
MboI	GATC	3 cut(s)	582, 1033, 1314
MboII	GAAGA	5 cut(s)	18, 152, 155, 1052, 1300
MfeI	CAATTG	2 cut(s)	307, 399
MhlI	GDGCHC	3 cut(s)	440, 703, 1027
MlsI	TGGCCA	1 cut(s)	124
MluNI	TGGCCA	1 cut(s)	124
MlyI	GAGTC	5 cut(s)	62, 284, 424, 425, 620
MmeI	TCCRAC	2 cut(s)	267, 1348
Mox20I	TGGCCA	1 cut(s)	124
Mph1103I	ATGCAT	2 cut(s)	908, 1015
MroXI	GAANNNNTTC	1 cut(s)	318
MscI	TGGCCA	1 cut(s)	124
MseI	TTAA	4 cut(s)	546, 735, 756, 783
MslI	CAYNNNNRTG	3 cut(s)	245, 911, 939
Msp20I	TGGCCA	1 cut(s)	124
MspA1I	CMGCKG	2 cut(s)	120, 953
MunI	CAATTG	2 cut(s)	307, 399
Mva1269I	GAATGC	1 cut(s)	530
MwoI	GCNNNNNNNGC	3 cut(s)	217, 384, 596
NdeI	CATATG	1 cut(s)	902
NdeII	GATC	3 cut(s)	582, 1033, 1314
NheI	GCTAGC	2 cut(s)	137, 595
NlaIII	CATG	6 cut(s)	175, 699, 807, 1180, 1199, 1332
NlaIV	GGNNCC	4 cut(s)	252, 526, 701, 847
NmuCI	GTSAC	3 cut(s)	81, 279, 1382
NsiI	ATGCAT	2 cut(s)	908, 1015
PaeR7I	CTCGAG	1 cut(s)	433
PciSI	GCTCTTC	2 cut(s)	165, 1283
PctI	GAATGC	1 cut(s)	530
PdmI	GAANNNNTTC	1 cut(s)	318
PfeI	GAWTC	5 cut(s)	101, 269, 455, 604, 816
PkrI	GCNGC	5 cut(s)	112, 199, 301, 925, 1413
PleI	GAGTC	5 cut(s)	61, 284, 423, 425, 619
PpsI	GAGTC	5 cut(s)	61, 284, 423, 425, 619
Ppu21I	YACGTR	1 cut(s)	1049
PsiI	TTATAA	1 cut(s)	204
PspN4I	GGNNCC	4 cut(s)	252, 526, 701, 847
PspOMI	GGGCCC	1 cut(s)	699
PspPI	GGNCC	2 cut(s)	699, 700
PspXI	VCTCGAGB	1 cut(s)	433
PstNI	CAGNNNCTG	1 cut(s)	874
PvuII	CAGCTG	2 cut(s)	120, 953
RsaI	GTAC	4 cut(s)	150, 176, 957, 1047
RsaNI	GTAC	4 cut(s)	149, 175, 956, 1046
RseI	CAYNNNNRTG	3 cut(s)	245, 911, 939
SalI	GTCGAC	1 cut(s)	428
SapI	GCTCTTC	2 cut(s)	165, 1283
SaqAI	TTAA	4 cut(s)	546, 735, 756, 783
SatI	GCNGC	5 cut(s)	111, 198, 300, 924, 1412
Sau3AI	GATC	3 cut(s)	582, 1033, 1314
Sau96I	GGNCC	2 cut(s)	699, 700
SchI	GAGTC	5 cut(s)	62, 284, 424, 425, 620
SduI	GDGCHC	3 cut(s)	440, 703, 1027
SfaNI	GCATC	6 cut(s)	490, 564, 1022, 1255, 1302, 1339
SfcI	CTRYAG	1 cut(s)	927
Sfr274I	CTCGAG	1 cut(s)	433
SlaI	CTCGAG	1 cut(s)	433
SmiMI	CAYNNNNRTG	3 cut(s)	245, 911, 939
SmlI	CTYRAG	2 cut(s)	433, 608
SmoI	CTYRAG	2 cut(s)	433, 608
SsiI	CCGC	2 cut(s)	299, 528
SspMI	CTAG	5 cut(s)	47, 60, 138, 596, 1446
StyI	CCWWGG	2 cut(s)	91, 762
TaaI	ACNGT	3 cut(s)	285, 1189, 1388
TaiI	ACGT	2 cut(s)	970, 1051
TaqI	TCGA	3 cut(s)	51, 429, 434
TauI	GCSGC	1 cut(s)	302
TfiI	GAWTC	5 cut(s)	101, 269, 455, 604, 816
Tru1I	TTAA	4 cut(s)	546, 735, 756, 783
Tru9I	TTAA	4 cut(s)	546, 735, 756, 783
TscAI	CASTG	9 cut(s)	139, 270, 288, 498, 1089, 1381, 1387, 1393, 1405
TseFI	GTSAC	3 cut(s)	81, 279, 1382
TseI	GCWGC	4 cut(s)	110, 197, 923, 1411
Tsp45I	GTSAC	3 cut(s)	81, 279, 1382
TspDTI	ATGAA	2 cut(s)	1193, 1317
TspGWI	ACGGA	1 cut(s)	483
TspRI	CASTG	9 cut(s)	139, 270, 288, 498, 1089, 1381, 1387, 1393, 1405
XapI	RAATTY	5 cut(s)	404, 638, 682, 1117, 1324
XcmI	CCANNNNNNNNNTGG	2 cut(s)	760, 1075
XhoI	CTCGAG	1 cut(s)	433
XmiI	GTMKAC	2 cut(s)	429, 1184
XmnI	GAANNNNTTC	1 cut(s)	318
XspI	CTAG	5 cut(s)	47, 60, 138, 596, 1446
Zsp2I	ATGCAT	2 cut(s)	908, 1015

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.