RLG00000013343

Organic solute transporter Ostalpha

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0006299
Physical Location & Seq
Forward (+)
30083036 .. 30086802
3767 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013343

Sequence Viewer

Length: 1380 bp
ATGTGGCCTGTCAATCTCAGTGCTGAATCTGCCGCAGCCATCAGCTGGCCAATCCTCAGTGCGAGCATGTTCGTACTAGTTGCACTGTTTCTCTCCATGTATCTTATCTTCGAGCACTTAGCTGCCTATAATCAGCCAGAGGAGCAGAAGTTTTTGATCGGGCTTATTCTGATGGTTCCTGTTTATGCCCTAGAATCATTTGTGTCACTGTTGGACTCAAATGCTGCATTCAACTGGGAAGCAATCCGGGACTGCTACGAGGCCTTTGCATTATATTGTTTTGAGAGATACTTGATAGCCTGCTTAGGTGGTGAGAAGCGTACAATTGAATTTATGGAGAGTCAGAGCATAGTCGATTCGAGCACTCCTCTTTTGAAAGAAGAATATGCTTATGGAGTTGTTGAACATCCTTTCCCGCTGAATTGCTTTATAAGAGACTGGGATCTTGGTCCGGCATTCTATTATGCTGTGAAAGTAGGCATCGTTCAGTATATGATACTGAAATTGATCTGTGCTCTGCTAGCCATGATTCTTCAAAGTTTTGGTGTTTATGGAGAAGGAAAGTTTGACTGGAGATATGGCTACCCTTACTTGGCAGTTGTTCTTAATTTTAGTCAGACATGGGCCCTTTACTGCCTTGTGCAGTTCTATTCTGTCATCAAGGACAAACTACAACCTATTAAACCACTGGCGAAGTTTTTAACTTTTAAGTCAATTGTATTCTTGACATGGTGGCAAGGTGTAGCTGTTGCGTTTCTTTTCTCTATGGGTGCCTTTAAAGGGGCCTTAGCGCAGGAGCTGAAAACCCGCATACAAGATTACATCATATGCATAGAGATGGGTGTTGCTGCTGTGGTCCACCTATATGTCTTTCCAGCAGTACCTTACAAACGTGGAGAAAGATGTGTTCGCAATGTTGCTGTGATGGCTGACTATGCATCACTAGGAGCGCCTCCTGATCCAGAGGAGGTTCGTGATTGTGAAAGATCAACAAGAATTCATCTACAACGGCATGATGAGAGAGAAAAGCGCTTGAATTTCCCCCAGAGTGTTCGTGACGTGATTTTTGGAAGTGGAGAAATTATTGTTGATGACATGAAGTATACAGTTTCGCATGTGGTAGAACCAGTTGAAAGAGGATTTGCAAAGATAAATAAAACCTTGCATCAGATATCTGAGAATGTGAAACGCCACGAGGAGCAGAGGAGGAGTGCCAAGGATGATAGTTATCTTATCCCCTTGAGTTCATGGAACAGAGAATTTTCTGAAGTCCATGAGAATCTTGTTGAAGGGAGTGTCAGTGACAGTGGTTTGTCCAATGGGAGGAGACAGCACACCCAGTCTAAAGCTTCAACTTCTCGTCCAAGAGCAGGGAGATGA

Protein Analysis

460

Amino Acids

52.41

Weight (kDa)

6.24

Isoelectric Point (pI)

44.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Solute_trans_a PF03619 19 - 296 6.1e-91 Organic solute transporter Ostalpha
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 431
AccB1I GGYRCC 1 cut(s) 770
AccB7I CCANNNNNTGG 1 cut(s) 45
AccI GTMKAC 1 cut(s) 1103
AciI CCGC 3 cut(s) 33, 416, 808
AclWI GGATC 2 cut(s) 450, 953
AcoI YGGCCR 1 cut(s) 47
AcsI RAATTY 4 cut(s) 329, 996, 1036, 1259
AcuI CTGAAG 1 cut(s) 1287
AfaI GTAC 3 cut(s) 75, 322, 882
AfeI AGCGCT 1 cut(s) 1031
AfiI CCNNNNNNNGG 5 cut(s) 45, 592, 780, 1323, 1370
AgsI TTSAA 9 cut(s) 232, 329, 376, 404, 536, 1036, 1133, 1289, 1353
AhlI ACTAGT 1 cut(s) 76
AjiI CACGTC 1 cut(s) 1060
AloI GAACNNNNNNTCC 2 cut(s) 396, 428
AluBI AGCT 5 cut(s) 45, 122, 746, 799, 1349
AluI AGCT 5 cut(s) 45, 122, 746, 799, 1349
Alw21I GWGCWC 3 cut(s) 117, 365, 517
Alw26I GTCTC 2 cut(s) 429, 1321
AlwI GGATC 2 cut(s) 450, 953
AlwNI CAGNNNCTG 1 cut(s) 799
Aor51HI AGCGCT 1 cut(s) 1031
AoxI GGCC 5 cut(s) 5, 47, 261, 624, 783
ApaI GGGCCC 1 cut(s) 628
ApeKI GCWGC 4 cut(s) 35, 122, 224, 848
ApoI RAATTY 4 cut(s) 329, 996, 1036, 1259
ArsI GACNNNNNNTTYG 2 cut(s) 1049, 1081
AspLEI GCGC 3 cut(s) 793, 952, 1032
AspS9I GGNCC 5 cut(s) 449, 624, 625, 783, 856
AsuC2I CCSGG 1 cut(s) 248
AsuHPI GGTGA 1 cut(s) 323
AsuNHI GCTAGC 1 cut(s) 520
AvaII GGWCC 2 cut(s) 449, 856
BaeGI GKGCMC 1 cut(s) 628
BalI TGGCCA 1 cut(s) 49
BanI GGYRCC 1 cut(s) 770
BanII GRGCYC 1 cut(s) 628
BarI GAAGNNNNNNTAC 2 cut(s) 92, 124
BauI CACGAG 1 cut(s) 1193
Bbv12I GWGCWC 3 cut(s) 117, 365, 517
BbvI GCAGC 4 cut(s) 47, 109, 211, 835
BccI CCATC 4 cut(s) 47, 166, 832, 919
BceAI ACGGC 1 cut(s) 1025
BcgI CGANNNNNNTGC 2 cut(s) 248, 282
BcnI CCSGG 1 cut(s) 248
BcoDI GTCTC 2 cut(s) 429, 1321
BcuI ACTAGT 1 cut(s) 76
BfaI CTAG 4 cut(s) 77, 191, 521, 944
BfoI RGCGCY 2 cut(s) 953, 1033
BisI GCNGC 5 cut(s) 33, 36, 123, 225, 849
BlsI GCNGC 5 cut(s) 34, 37, 124, 226, 850
Bme1390I CCNGG 1 cut(s) 248
Bme18I GGWCC 2 cut(s) 449, 856
BmgBI CACGTC 1 cut(s) 1060
BmgT120I GGNCC 5 cut(s) 449, 624, 625, 783, 856
BmiI GGNNCC 4 cut(s) 177, 626, 772, 784
BmrFI CCNGG 1 cut(s) 248
BmrI ACTGGG 3 cut(s) 244, 448, 1333
BmsI GCATC 3 cut(s) 489, 947, 1174
BmtI GCTAGC 1 cut(s) 524
BmuI ACTGGG 3 cut(s) 244, 448, 1333
BplI GAGNNNNNCTC 2 cut(s) 352, 384
BpmI CTGGAG 1 cut(s) 592
Bpu10I CCTNAGC 2 cut(s) 304, 787
BpuEI CTTGAG 1 cut(s) 1261
BpuMI CCSGG 1 cut(s) 248
BsaBI GATNNNNATC 1 cut(s) 1227
BsaJI CCNNGG 1 cut(s) 1215
BsaXI ACNNNNNCTCC 4 cut(s) 134, 164, 1285, 1315
Bsc4I CCNNNNNNNGG 5 cut(s) 45, 592, 780, 1323, 1370
Bse1I ACTGG 6 cut(s) 239, 443, 575, 693, 1127, 1339
Bse3DI GCAATG 1 cut(s) 919
Bse8I GATNNNNATC 1 cut(s) 1227
BseDI CCNNGG 1 cut(s) 1215
BseGI GGATG 2 cut(s) 406, 1225
BseJI GATNNNNATC 1 cut(s) 1227
BseLI CCNNNNNNNGG 5 cut(s) 45, 592, 780, 1323, 1370
BseMI GCAATG 1 cut(s) 919
BseMII CTCAG 3 cut(s) 31, 70, 1167
BseNI ACTGG 6 cut(s) 239, 443, 575, 693, 1127, 1339
BseRI GAGGAG 7 cut(s) 155, 357, 980, 1211, 1219, 1222, 1339
BseSI GKGCMC 1 cut(s) 628
BseXI GCAGC 4 cut(s) 47, 109, 211, 835
BsgI GTGCAG 1 cut(s) 662
BshFI GGCC 5 cut(s) 7, 49, 263, 626, 785
BshNI GGYRCC 1 cut(s) 770
BsiHKAI GWGCWC 3 cut(s) 117, 365, 517
BsiSI CCGG 2 cut(s) 247, 452
BslFI GGGAC 1 cut(s) 263
BslI CCNNNNNNNGG 5 cut(s) 45, 592, 780, 1323, 1370
BsmAI GTCTC 2 cut(s) 429, 1321
BsmFI GGGAC 1 cut(s) 263
BsmI GAATGC 2 cut(s) 227, 455
BsnI GGCC 5 cut(s) 7, 49, 263, 626, 785
Bsp120I GGGCCC 1 cut(s) 624
Bsp1286I GDGCHC 4 cut(s) 117, 365, 517, 628
Bsp143I GATC 5 cut(s) 156, 442, 507, 958, 986
BspACI CCGC 3 cut(s) 33, 416, 808
BspANI GGCC 5 cut(s) 7, 49, 263, 626, 785
BspCNI CTCAG 3 cut(s) 30, 69, 1168
BspLI GGNNCC 4 cut(s) 177, 626, 772, 784
BspOI GCTAGC 1 cut(s) 524
BspPI GGATC 2 cut(s) 450, 953
BspT107I GGYRCC 1 cut(s) 770
BsrDI GCAATG 1 cut(s) 919
BsrI ACTGG 6 cut(s) 239, 443, 575, 693, 1127, 1339
BssECI CCNNGG 1 cut(s) 1215
BssMI GATC 5 cut(s) 156, 442, 507, 958, 986
BssNAI GTATAC 1 cut(s) 1104
BssSI CACGAG 1 cut(s) 1193
BssT1I CCWWGG 1 cut(s) 1215
Bst1107I GTATAC 1 cut(s) 1104
Bst2BI CACGAG 1 cut(s) 1193
Bst4CI ACNGT 4 cut(s) 87, 210, 1108, 1307
BstC8I GCNNGC 4 cut(s) 47, 64, 301, 522
BstDEI CTNAG 6 cut(s) 17, 56, 118, 304, 787, 1176
BstF5I GGATG 2 cut(s) 406, 1225
BstH2I RGCGCY 2 cut(s) 953, 1033
BstHHI GCGC 3 cut(s) 793, 952, 1032
BstKTI GATC 5 cut(s) 159, 445, 510, 961, 989
BstMAI GTCTC 2 cut(s) 429, 1321
BstMBI GATC 5 cut(s) 156, 442, 507, 958, 986
BstMWI GCNNNNNNNGC 5 cut(s) 29, 142, 521, 926, 935
BstNSI RCATGY 2 cut(s) 70, 1118
BstSCI CCNGG 1 cut(s) 246
BstSLI GKGCMC 1 cut(s) 628
BstV1I GCAGC 4 cut(s) 47, 109, 211, 835
BstX2I RGATCY 1 cut(s) 442
BstYI RGATCY 1 cut(s) 442
BstZ17I GTATAC 1 cut(s) 1104
BsuRI GGCC 5 cut(s) 7, 49, 263, 626, 785
BtrI CACGTC 1 cut(s) 1060
BtsCI GGATG 2 cut(s) 406, 1225
BtsIMutI CAGTG 7 cut(s) 25, 64, 83, 206, 686, 1306, 1312
Cac8I GCNNGC 4 cut(s) 47, 64, 301, 522
CaiI CAGNNNCTG 1 cut(s) 799
CfoI GCGC 3 cut(s) 793, 952, 1032
Cfr13I GGNCC 5 cut(s) 449, 624, 625, 783, 856
Csp6I GTAC 3 cut(s) 74, 321, 881
CviQI GTAC 3 cut(s) 74, 321, 881
DdeI CTNAG 6 cut(s) 17, 56, 118, 304, 787, 1176
DpnI GATC 5 cut(s) 158, 444, 509, 960, 988
DpnII GATC 5 cut(s) 156, 442, 507, 958, 986
DraI TTTAAA 1 cut(s) 778
EaeI YGGCCR 1 cut(s) 47
Eco130I CCWWGG 1 cut(s) 1215
Eco147I AGGCCT 1 cut(s) 263
Eco24I GRGCYC 1 cut(s) 628
Eco32I GATATC 1 cut(s) 1173
Eco47I GGWCC 2 cut(s) 449, 856
Eco47III AGCGCT 1 cut(s) 1031
Eco57I CTGAAG 1 cut(s) 1287
EcoO109I RGGNCCY 2 cut(s) 625, 783
EcoRI GAATTC 1 cut(s) 996
EcoRV GATATC 1 cut(s) 1173
EcoT14I CCWWGG 1 cut(s) 1215
EcoT22I ATGCAT 2 cut(s) 833, 940
EcoT38I GRGCYC 1 cut(s) 628
ErhI CCWWGG 1 cut(s) 1215
FaqI GGGAC 1 cut(s) 263
FauI CCCGC 2 cut(s) 423, 815
FauNDI CATATG 1 cut(s) 827
FblI GTMKAC 1 cut(s) 1103
Fnu4HI GCNGC 5 cut(s) 33, 36, 123, 225, 849
FokI GGATG 2 cut(s) 393, 1232
FriOI GRGCYC 1 cut(s) 628
Fsp4HI GCNGC 5 cut(s) 33, 36, 123, 225, 849
FspBI CTAG 4 cut(s) 77, 191, 521, 944
GlaI GCGC 3 cut(s) 792, 951, 1031
GluI GCNGC 5 cut(s) 33, 36, 123, 225, 849
GsuI CTGGAG 1 cut(s) 592
HaeII RGCGCY 2 cut(s) 953, 1033
HaeIII GGCC 5 cut(s) 7, 49, 263, 626, 785
HapII CCGG 2 cut(s) 247, 452
HhaI GCGC 3 cut(s) 793, 952, 1032
Hin6I GCGC 3 cut(s) 791, 950, 1030
HinP1I GCGC 3 cut(s) 791, 950, 1030
HindIII AAGCTT 1 cut(s) 1347
HinfI GANTC 7 cut(s) 26, 194, 215, 340, 356, 529, 1279
HpaII CCGG 2 cut(s) 247, 452
HphI GGTGA 1 cut(s) 323
Hpy166II GTNNAC 2 cut(s) 859, 1104
Hpy188I TCNGA 6 cut(s) 171, 345, 618, 1170, 1177, 1267
Hpy188III TCNNGA 5 cut(s) 724, 956, 962, 974, 1055
Hpy8I GTNNAC 2 cut(s) 859, 1104
HpyAV CCTTC 2 cut(s) 551, 1283
HpyCH4III ACNGT 4 cut(s) 87, 210, 1108, 1307
HpyCH4IV ACGT 2 cut(s) 892, 1059
HpyCH4V TGCA 8 cut(s) 83, 227, 269, 643, 831, 938, 1145, 1165
HpyF10VI GCNNNNNNNGC 5 cut(s) 29, 142, 521, 926, 935
HpyF3I CTNAG 6 cut(s) 17, 56, 118, 304, 787, 1176
HpySE526I ACGT 2 cut(s) 892, 1059
HspAI GCGC 3 cut(s) 791, 950, 1030
Kzo9I GATC 5 cut(s) 156, 442, 507, 958, 986
LmnI GCTCC 4 cut(s) 142, 796, 947, 1198
Lsp1109I GCAGC 4 cut(s) 47, 109, 211, 835
LweI GCATC 3 cut(s) 489, 947, 1174
MaeI CTAG 4 cut(s) 77, 191, 521, 944
MaeII ACGT 2 cut(s) 892, 1059
MaeIII GTNAC 3 cut(s) 204, 1055, 1301
MalI GATC 5 cut(s) 158, 444, 509, 960, 988
MboI GATC 5 cut(s) 156, 442, 507, 958, 986
MboII GAAGA 3 cut(s) 100, 392, 524
MfeI CAATTG 2 cut(s) 324, 714
MflI RGATCY 1 cut(s) 442
MhlI GDGCHC 4 cut(s) 117, 365, 517, 628
MlsI TGGCCA 1 cut(s) 49
MluNI TGGCCA 1 cut(s) 49
MlyI GAGTC 2 cut(s) 209, 349
MmeI TCCRAC 1 cut(s) 192
Mox20I TGGCCA 1 cut(s) 49
Mph1103I ATGCAT 2 cut(s) 833, 940
MscI TGGCCA 1 cut(s) 49
MseI TTAA 5 cut(s) 606, 681, 701, 708, 777
MslI CAYNNNNRTG 2 cut(s) 836, 864
Msp20I TGGCCA 1 cut(s) 49
MspA1I CMGCKG 2 cut(s) 45, 418
MspI CCGG 2 cut(s) 247, 452
MspR9I CCNGG 1 cut(s) 248
MunI CAATTG 2 cut(s) 324, 714
Mva1269I GAATGC 2 cut(s) 227, 455
MwoI GCNNNNNNNGC 5 cut(s) 29, 142, 521, 926, 935
NciI CCSGG 1 cut(s) 248
NdeI CATATG 1 cut(s) 827
NdeII GATC 5 cut(s) 156, 442, 507, 958, 986
NheI GCTAGC 1 cut(s) 520
NlaIV GGNNCC 4 cut(s) 177, 626, 772, 784
NmuCI GTSAC 3 cut(s) 204, 1055, 1301
NsiI ATGCAT 2 cut(s) 833, 940
NspI RCATGY 2 cut(s) 70, 1118
PceI AGGCCT 1 cut(s) 263
PctI GAATGC 2 cut(s) 227, 455
PfeI GAWTC 5 cut(s) 26, 194, 356, 529, 1279
PflMI CCANNNNNTGG 1 cut(s) 45
PfoI TCCNGGA 1 cut(s) 246
PkrI GCNGC 5 cut(s) 34, 37, 124, 226, 850
PleI GAGTC 2 cut(s) 209, 348
PpsI GAGTC 2 cut(s) 209, 348
PsiI TTATAA 1 cut(s) 431
PspN4I GGNNCC 4 cut(s) 177, 626, 772, 784
PspOMI GGGCCC 1 cut(s) 624
PspPI GGNCC 5 cut(s) 449, 624, 625, 783, 856
PsrI GAACNNNNNNTAC 2 cut(s) 468, 500
PstNI CAGNNNCTG 1 cut(s) 799
PsuI RGATCY 1 cut(s) 442
PvuII CAGCTG 1 cut(s) 45
RsaI GTAC 3 cut(s) 75, 322, 882
RsaNI GTAC 3 cut(s) 74, 321, 881
RseI CAYNNNNRTG 2 cut(s) 836, 864
SaqAI TTAA 5 cut(s) 606, 681, 701, 708, 777
SatI GCNGC 5 cut(s) 33, 36, 123, 225, 849
Sau3AI GATC 5 cut(s) 156, 442, 507, 958, 986
Sau96I GGNCC 5 cut(s) 449, 624, 625, 783, 856
SchI GAGTC 2 cut(s) 209, 349
ScrFI CCNGG 1 cut(s) 248
SduI GDGCHC 4 cut(s) 117, 365, 517, 628
SfaNI GCATC 3 cut(s) 489, 947, 1174
SinI GGWCC 2 cut(s) 449, 856
SmiMI CAYNNNNRTG 2 cut(s) 836, 864
SmlI CTYRAG 1 cut(s) 1240
SmoI CTYRAG 1 cut(s) 1240
SpeI ACTAGT 1 cut(s) 76
SseBI AGGCCT 1 cut(s) 263
SsiI CCGC 3 cut(s) 33, 416, 808
SspMI CTAG 4 cut(s) 77, 191, 521, 944
StuI AGGCCT 1 cut(s) 263
StyD4I CCNGG 1 cut(s) 246
StyI CCWWGG 1 cut(s) 1215
TaaI ACNGT 4 cut(s) 87, 210, 1108, 1307
TaiI ACGT 2 cut(s) 895, 1062
TaqI TCGA 3 cut(s) 111, 354, 359
TauI GCSGC 1 cut(s) 35
TfiI GAWTC 5 cut(s) 26, 194, 356, 529, 1279
Tru1I TTAA 5 cut(s) 606, 681, 701, 708, 777
Tru9I TTAA 5 cut(s) 606, 681, 701, 708, 777
TscAI CASTG 7 cut(s) 25, 64, 90, 213, 693, 1306, 1312
TseFI GTSAC 3 cut(s) 204, 1055, 1301
TseI GCWGC 4 cut(s) 35, 122, 224, 848
Tsp45I GTSAC 3 cut(s) 204, 1055, 1301
TspDTI ATGAA 3 cut(s) 989, 1112, 1236
TspRI CASTG 7 cut(s) 25, 64, 90, 213, 693, 1306, 1312
Van91I CCANNNNNTGG 1 cut(s) 45
VpaK11BI GGWCC 2 cut(s) 449, 856
XapI RAATTY 4 cut(s) 329, 996, 1036, 1259
XceI RCATGY 2 cut(s) 70, 1118
XmiI GTMKAC 1 cut(s) 1103
XspI CTAG 4 cut(s) 77, 191, 521, 944
Zsp2I ATGCAT 2 cut(s) 833, 940
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.