Rh6AG215700

Organic solute transporter Ostalpha

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0006299
Physical Location & Seq
Reverse (-)
38652120 .. 38658270
6151 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG215700.1

Sequence Viewer

Length: 1047 bp
ATGGAGAGTCAGAGCACAGTCGATTCGAGCACTCCTCTTTTGAAAGAAGAATATGCTTACGGAGTTGTTGAACATCCTTTCCCACTGAATTGCTTTATAAGAGACTGGGATCTTGGACCAGCATTCTATCATGCTGTGAAAGTAGGCATCGTTCAGTATATGATACTGAAATTGATCTGCGCTCTGCTAGCCATGATTCTTCAAAGTTTTGGTGTTTATGGAGAAGGAAAGTTTGACTGGAGATATGGCTACCCTTACTTGGCAGTTGTTCTTAACTTTAGTCAGACATGGGCCCTATACTGCCTTGTGCAGTTCTATTCTGTCATCAAGGACAAATTACAACCCATTAAACCACTGGCGAAGTTTCTAACTTTTAAGTCAATTGTATTCTTGACATGGTGGCAAGGTGTTGCTGTTGCGTTTCTTTTCTCTATGGGTGCCTTTAAAGGGGCCTTAGCGCAGGAGCTGAAAACCCGCATACAAGATTACATCATATGCATAGAGATGGGTGTTGCTGCTGTGGTCCACCTATATGTCTTCCCGGCAGTACCTTACAAACGTGGAGAAAGATGTGTTCGCAATGTTGCTGTGATGGCTGACTATGCATCACTAGGAGCGCCTCCTGATCCAGAGGAGGTTCGTGATTGTGAAAGATCAACAAGAATTCATCTACAGCGGCATGATGAGAGAGAAAAGCGCTTGAATTTCCCCCAGAGTGTTCGTGACGTGATTTTTGGAAGTGGAGAAATTATTGTTGATGACATGAAGTATACAGTTTCACATGTGGTAGAACCAGTTGAAAGAGGATTTGCAAAGATAAATAAAACCTTGCATCAGATATCTGAGAATGTGAAACGCCATGAGGAGCAGAGGAGGAGTGCCAAGGATGATAGTTATCTTATCCCCTTGAGTTCATGGAACAGAGAATTTTCTGAAGTCCATGAGAATCTTGTTGAAGGGAGTGTCAGTGACAGTGGTTTGTCCAATGGGAGGAGACAGCACACCCAGTCTAAAGCTTCAACTTCTCGTCCAAGAGCAGGGAGATGA

Protein Analysis

348

Amino Acids

39.76

Weight (kDa)

8.4

Isoelectric Point (pI)

43.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Solute_trans_a PF03619 23 - 185 4e-58 Organic solute transporter Ostalpha
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 98
AccB1I GGYRCC 1 cut(s) 437
AccI GTMKAC 1 cut(s) 770
AciI CCGC 2 cut(s) 475, 676
AclWI GGATC 2 cut(s) 117, 620
AcsI RAATTY 3 cut(s) 663, 703, 926
AcuI CTGAAG 1 cut(s) 954
AfaI GTAC 1 cut(s) 549
AfeI AGCGCT 1 cut(s) 698
AfiI CCNNNNNNNGG 4 cut(s) 259, 447, 990, 1037
AflIII ACRYGT 1 cut(s) 781
AgsI TTSAA 7 cut(s) 43, 71, 203, 703, 800, 956, 1020
AjiI CACGTC 1 cut(s) 727
AloI GAACNNNNNNTCC 2 cut(s) 63, 95
AluBI AGCT 2 cut(s) 466, 1016
AluI AGCT 2 cut(s) 466, 1016
Alw21I GWGCWC 2 cut(s) 17, 32
Alw26I GTCTC 2 cut(s) 96, 988
AlwI GGATC 2 cut(s) 117, 620
AlwNI CAGNNNCTG 1 cut(s) 466
Aor51HI AGCGCT 1 cut(s) 698
AoxI GGCC 2 cut(s) 291, 450
ApaI GGGCCC 1 cut(s) 295
ApeKI GCWGC 1 cut(s) 515
ApoI RAATTY 3 cut(s) 663, 703, 926
ArsI GACNNNNNNTTYG 2 cut(s) 716, 748
AspLEI GCGC 4 cut(s) 182, 460, 619, 699
AspS9I GGNCC 5 cut(s) 116, 291, 292, 450, 523
AsuC2I CCSGG 1 cut(s) 542
AsuNHI GCTAGC 1 cut(s) 187
AvaII GGWCC 2 cut(s) 116, 523
BaeGI GKGCMC 1 cut(s) 295
BanI GGYRCC 1 cut(s) 437
BanII GRGCYC 1 cut(s) 295
BbsI GAAGAC 1 cut(s) 529
Bbv12I GWGCWC 2 cut(s) 17, 32
BbvI GCAGC 1 cut(s) 502
BccI CCATC 2 cut(s) 499, 586
BcnI CCSGG 1 cut(s) 542
BcoDI GTCTC 2 cut(s) 96, 988
BfaI CTAG 2 cut(s) 188, 611
BfmI CTRYAG 1 cut(s) 671
BfoI RGCGCY 2 cut(s) 620, 700
BisI GCNGC 2 cut(s) 516, 677
BlsI GCNGC 2 cut(s) 517, 678
Bme1390I CCNGG 1 cut(s) 542
Bme18I GGWCC 2 cut(s) 116, 523
BmgBI CACGTC 1 cut(s) 727
BmgT120I GGNCC 5 cut(s) 116, 291, 292, 450, 523
BmiI GGNNCC 3 cut(s) 293, 439, 451
BmrFI CCNGG 1 cut(s) 542
BmrI ACTGGG 2 cut(s) 115, 1000
BmsI GCATC 3 cut(s) 156, 614, 841
BmtI GCTAGC 1 cut(s) 191
BmuI ACTGGG 2 cut(s) 115, 1000
BpiI GAAGAC 1 cut(s) 529
BplI GAGNNNNNCTC 2 cut(s) 19, 51
BpmI CTGGAG 1 cut(s) 259
Bpu10I CCTNAGC 1 cut(s) 454
BpuEI CTTGAG 1 cut(s) 928
BpuMI CCSGG 1 cut(s) 542
BsaBI GATNNNNATC 1 cut(s) 894
BsaJI CCNNGG 1 cut(s) 882
BsaXI ACNNNNNCTCC 2 cut(s) 952, 982
Bsc4I CCNNNNNNNGG 4 cut(s) 259, 447, 990, 1037
Bse1I ACTGG 5 cut(s) 110, 242, 360, 794, 1006
Bse3DI GCAATG 1 cut(s) 586
Bse8I GATNNNNATC 1 cut(s) 894
BseDI CCNNGG 1 cut(s) 882
BseGI GGATG 2 cut(s) 73, 892
BseJI GATNNNNATC 1 cut(s) 894
BseLI CCNNNNNNNGG 4 cut(s) 259, 447, 990, 1037
BseMI GCAATG 1 cut(s) 586
BseMII CTCAG 1 cut(s) 834
BseNI ACTGG 5 cut(s) 110, 242, 360, 794, 1006
BseRI GAGGAG 6 cut(s) 24, 647, 878, 886, 889, 1006
BseSI GKGCMC 1 cut(s) 295
BseXI GCAGC 1 cut(s) 502
BsgI GTGCAG 1 cut(s) 329
BshFI GGCC 2 cut(s) 293, 452
BshNI GGYRCC 1 cut(s) 437
BsiHKAI GWGCWC 2 cut(s) 17, 32
BsiSI CCGG 1 cut(s) 542
BslI CCNNNNNNNGG 4 cut(s) 259, 447, 990, 1037
BsmAI GTCTC 2 cut(s) 96, 988
BsmI GAATGC 1 cut(s) 122
BsnI GGCC 2 cut(s) 293, 452
Bsp120I GGGCCC 1 cut(s) 291
Bsp1286I GDGCHC 3 cut(s) 17, 32, 295
Bsp143I GATC 4 cut(s) 109, 174, 625, 653
BspACI CCGC 2 cut(s) 475, 676
BspANI GGCC 2 cut(s) 293, 452
BspCNI CTCAG 1 cut(s) 835
BspLI GGNNCC 3 cut(s) 293, 439, 451
BspOI GCTAGC 1 cut(s) 191
BspPI GGATC 2 cut(s) 117, 620
BspT107I GGYRCC 1 cut(s) 437
BsrDI GCAATG 1 cut(s) 586
BsrI ACTGG 5 cut(s) 110, 242, 360, 794, 1006
BssECI CCNNGG 1 cut(s) 882
BssMI GATC 4 cut(s) 109, 174, 625, 653
BssNAI GTATAC 1 cut(s) 771
BssT1I CCWWGG 1 cut(s) 882
Bst1107I GTATAC 1 cut(s) 771
Bst4CI ACNGT 3 cut(s) 19, 775, 974
BstC8I GCNNGC 1 cut(s) 189
BstDEI CTNAG 2 cut(s) 454, 843
BstF5I GGATG 2 cut(s) 73, 892
BstH2I RGCGCY 2 cut(s) 620, 700
BstHHI GCGC 4 cut(s) 182, 460, 619, 699
BstKTI GATC 4 cut(s) 112, 177, 628, 656
BstMAI GTCTC 2 cut(s) 96, 988
BstMBI GATC 4 cut(s) 109, 174, 625, 653
BstMWI GCNNNNNNNGC 3 cut(s) 188, 593, 602
BstNSI RCATGY 1 cut(s) 785
BstSCI CCNGG 1 cut(s) 540
BstSFI CTRYAG 1 cut(s) 671
BstSLI GKGCMC 1 cut(s) 295
BstV1I GCAGC 1 cut(s) 502
BstV2I GAAGAC 1 cut(s) 529
BstX2I RGATCY 1 cut(s) 109
BstYI RGATCY 1 cut(s) 109
BstZ17I GTATAC 1 cut(s) 771
BsuRI GGCC 2 cut(s) 293, 452
BtrI CACGTC 1 cut(s) 727
BtsCI GGATG 2 cut(s) 73, 892
BtsIMutI CAGTG 4 cut(s) 83, 353, 973, 979
Cac8I GCNNGC 1 cut(s) 189
CaiI CAGNNNCTG 1 cut(s) 466
CfoI GCGC 4 cut(s) 182, 460, 619, 699
Cfr13I GGNCC 5 cut(s) 116, 291, 292, 450, 523
Csp6I GTAC 1 cut(s) 548
CviJI RGCY 7 cut(s) 191, 249, 293, 452, 466, 596, 1016
CviKI_1 RGCY 7 cut(s) 191, 249, 293, 452, 466, 596, 1016
CviQI GTAC 1 cut(s) 548
DdeI CTNAG 2 cut(s) 454, 843
DpnI GATC 4 cut(s) 111, 176, 627, 655
DpnII GATC 4 cut(s) 109, 174, 625, 653
DraI TTTAAA 1 cut(s) 445
Eco130I CCWWGG 1 cut(s) 882
Eco24I GRGCYC 1 cut(s) 295
Eco32I GATATC 1 cut(s) 840
Eco47I GGWCC 2 cut(s) 116, 523
Eco47III AGCGCT 1 cut(s) 698
Eco57I CTGAAG 1 cut(s) 954
EcoO109I RGGNCCY 2 cut(s) 292, 450
EcoRI GAATTC 1 cut(s) 663
EcoRV GATATC 1 cut(s) 840
EcoT14I CCWWGG 1 cut(s) 882
EcoT22I ATGCAT 2 cut(s) 500, 607
EcoT38I GRGCYC 1 cut(s) 295
ErhI CCWWGG 1 cut(s) 882
FauI CCCGC 1 cut(s) 482
FauNDI CATATG 1 cut(s) 494
FblI GTMKAC 1 cut(s) 770
Fnu4HI GCNGC 2 cut(s) 516, 677
FokI GGATG 2 cut(s) 60, 899
FriOI GRGCYC 1 cut(s) 295
Fsp4HI GCNGC 2 cut(s) 516, 677
FspBI CTAG 2 cut(s) 188, 611
GlaI GCGC 4 cut(s) 181, 459, 618, 698
GluI GCNGC 2 cut(s) 516, 677
GsuI CTGGAG 1 cut(s) 259
HaeII RGCGCY 2 cut(s) 620, 700
HaeIII GGCC 2 cut(s) 293, 452
HapII CCGG 1 cut(s) 542
HhaI GCGC 4 cut(s) 182, 460, 619, 699
Hin6I GCGC 4 cut(s) 180, 458, 617, 697
HinP1I GCGC 4 cut(s) 180, 458, 617, 697
HindIII AAGCTT 1 cut(s) 1014
HinfI GANTC 4 cut(s) 7, 23, 196, 946
HpaII CCGG 1 cut(s) 542
Hpy166II GTNNAC 2 cut(s) 526, 771
Hpy188I TCNGA 5 cut(s) 12, 285, 837, 844, 934
Hpy188III TCNNGA 5 cut(s) 391, 623, 629, 641, 722
Hpy8I GTNNAC 2 cut(s) 526, 771
HpyAV CCTTC 2 cut(s) 218, 950
HpyCH4III ACNGT 3 cut(s) 19, 775, 974
HpyCH4IV ACGT 2 cut(s) 559, 726
HpyCH4V TGCA 5 cut(s) 310, 498, 605, 812, 832
HpyF10VI GCNNNNNNNGC 3 cut(s) 188, 593, 602
HpyF3I CTNAG 2 cut(s) 454, 843
HpySE526I ACGT 2 cut(s) 559, 726
HspAI GCGC 4 cut(s) 180, 458, 617, 697
Kzo9I GATC 4 cut(s) 109, 174, 625, 653
LmnI GCTCC 3 cut(s) 463, 614, 865
Lsp1109I GCAGC 1 cut(s) 502
LweI GCATC 3 cut(s) 156, 614, 841
MaeI CTAG 2 cut(s) 188, 611
MaeII ACGT 2 cut(s) 559, 726
MaeIII GTNAC 2 cut(s) 722, 968
MalI GATC 4 cut(s) 111, 176, 627, 655
MboI GATC 4 cut(s) 109, 174, 625, 653
MboII GAAGA 3 cut(s) 59, 191, 529
MfeI CAATTG 1 cut(s) 381
MflI RGATCY 1 cut(s) 109
MhlI GDGCHC 3 cut(s) 17, 32, 295
MluCI AATT 8 cut(s) 88, 170, 335, 381, 663, 703, 747, 926
MlyI GAGTC 1 cut(s) 16
MnlI CCTC 9 cut(s) 45, 625, 628, 630, 797, 856, 864, 867, 984
Mph1103I ATGCAT 2 cut(s) 500, 607
MseI TTAA 4 cut(s) 273, 348, 375, 444
MslI CAYNNNNRTG 2 cut(s) 503, 531
MspA1I CMGCKG 1 cut(s) 676
MspI CCGG 1 cut(s) 542
MspR9I CCNGG 1 cut(s) 542
MunI CAATTG 1 cut(s) 381
Mva1269I GAATGC 1 cut(s) 122
MwoI GCNNNNNNNGC 3 cut(s) 188, 593, 602
NciI CCSGG 1 cut(s) 542
NdeI CATATG 1 cut(s) 494
NdeII GATC 4 cut(s) 109, 174, 625, 653
NheI GCTAGC 1 cut(s) 187
NlaIV GGNNCC 3 cut(s) 293, 439, 451
NmuCI GTSAC 2 cut(s) 722, 968
NsiI ATGCAT 2 cut(s) 500, 607
NspI RCATGY 1 cut(s) 785
PciI ACATGT 1 cut(s) 781
PctI GAATGC 1 cut(s) 122
PfeI GAWTC 3 cut(s) 23, 196, 946
PkrI GCNGC 2 cut(s) 517, 678
PleI GAGTC 1 cut(s) 15
PpsI GAGTC 1 cut(s) 15
PscI ACATGT 1 cut(s) 781
PsiI TTATAA 1 cut(s) 98
PspN4I GGNNCC 3 cut(s) 293, 439, 451
PspOMI GGGCCC 1 cut(s) 291
PspPI GGNCC 5 cut(s) 116, 291, 292, 450, 523
PsrI GAACNNNNNNTAC 2 cut(s) 135, 167
PstNI CAGNNNCTG 1 cut(s) 466
PsuI RGATCY 1 cut(s) 109
RsaI GTAC 1 cut(s) 549
RsaNI GTAC 1 cut(s) 548
RseI CAYNNNNRTG 2 cut(s) 503, 531
SaqAI TTAA 4 cut(s) 273, 348, 375, 444
SatI GCNGC 2 cut(s) 516, 677
Sau3AI GATC 4 cut(s) 109, 174, 625, 653
Sau96I GGNCC 5 cut(s) 116, 291, 292, 450, 523
SchI GAGTC 1 cut(s) 16
ScrFI CCNGG 1 cut(s) 542
SduI GDGCHC 3 cut(s) 17, 32, 295
SetI ASST 9 cut(s) 409, 468, 531, 553, 562, 639, 729, 830, 1018
SfaNI GCATC 3 cut(s) 156, 614, 841
SfcI CTRYAG 1 cut(s) 671
SinI GGWCC 2 cut(s) 116, 523
SmiMI CAYNNNNRTG 2 cut(s) 503, 531
SmlI CTYRAG 1 cut(s) 907
SmoI CTYRAG 1 cut(s) 907
Sse9I AATT 8 cut(s) 88, 170, 335, 381, 663, 703, 747, 926
SsiI CCGC 2 cut(s) 475, 676
SspMI CTAG 2 cut(s) 188, 611
StyD4I CCNGG 1 cut(s) 540
StyI CCWWGG 1 cut(s) 882
TaaI ACNGT 3 cut(s) 19, 775, 974
TaiI ACGT 2 cut(s) 562, 729
TaqI TCGA 2 cut(s) 21, 26
TasI AATT 8 cut(s) 88, 170, 335, 381, 663, 703, 747, 926
TauI GCSGC 1 cut(s) 679
TfiI GAWTC 3 cut(s) 23, 196, 946
Tru1I TTAA 4 cut(s) 273, 348, 375, 444
Tru9I TTAA 4 cut(s) 273, 348, 375, 444
TscAI CASTG 4 cut(s) 90, 360, 973, 979
TseFI GTSAC 2 cut(s) 722, 968
TseI GCWGC 1 cut(s) 515
Tsp45I GTSAC 2 cut(s) 722, 968
TspDTI ATGAA 3 cut(s) 656, 779, 903
TspGWI ACGGA 1 cut(s) 75
TspRI CASTG 4 cut(s) 90, 360, 973, 979
VpaK11BI GGWCC 2 cut(s) 116, 523
XapI RAATTY 3 cut(s) 663, 703, 926
XceI RCATGY 1 cut(s) 785
XcmI CCANNNNNNNNNTGG 1 cut(s) 352
XmiI GTMKAC 1 cut(s) 770
XspI CTAG 2 cut(s) 188, 611
Zsp2I ATGCAT 2 cut(s) 500, 607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.