Rh6CG223200

Organic solute transporter Ostalpha

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0006299
Physical Location & Seq
Reverse (-)
37198402 .. 37202245
3844 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG223200.1

Sequence Viewer

Length: 1452 bp
ATGGGATGGAGGGGCAGGTTGTGTTCTTCCTTTTTCCTTTTCACTTTGGTCTCCTCCAGCAGAGTAGGAGACATGTGGCCTGTCAATCTCAGTGCTGAATCTGCCGTAGCCATCAGCTGGCCAATCCTCAGTGCGAGCATGTTCGTACTAGTTGCACTGTTTCTCTCCATGTATCTTATCTTCGAGCACTTAGCTGCCTATAATCAGCCAGAGGAGCAGAAGTTTTTGATCGGGCTTATTCTGATGGTTCCTGTTTATGCCCTAGAATCATTTGTGTCACTGTTGGACTCAAATGCTGCATTCAACTGGGAAGCAATCCGGGACTGCTACGAGGCCTTTGCATTATATTGTTTTGAGAGATACTTGATAGCCTGCTTAGGTGGTGAGAAGCGTACAATTGAATTTATGGAGAGTCAGAGCATAGTCGATTCGAGCACTCCTCTTTTGAAAGAAGAATACGCTTATGGAGTTGTTGAACATCCTTTCCCACTGAATTGCTTTATAAGAGACTGGGATCTTGGTCCAGCATTCTATCATGCTGTGAAAGTAGGCATCGTTCAGTATATGATACTGAAATTGATCTGCGCTCTGCTAGCCATGATTCTTCAAAGTTTTGGTGTTTATGGAGAAGGAAAGTTTGACTGGAGATATGGCTACCCTTACTTGGCAGTTGTTCTTAACTTTAGTCAGACATGGGCCCTATACTGCCTTGTGCAGTTCTATTCTGTCATCAAGGACAAACTACAACCTATTAAACCACTGGCAAAGTTTCTAACTTTTAAGTCAATTGTATTCTTGACATGGTGGCAAGGTGTTGCTGTTGCGTTTCTTTTCTCTATGGGTGCCTTTAAAGGGGCCTTGGCGCAGGAGCTGAAAACCCGCATACAAGATTACATCATATGCATAGAGATGGGTGTTGCTGCTGTGGTCCACCTATATGTCTTCCCGGCAGTACCTTACAAACGTGGAGAAAGATGTGTTCGCAATGTTGCTGTGATGGCTGACTATGCATCACTAGGAGCGCCTCCCGATCCAGAGGAGGTTCGTGATTGTGAAAGATCAACAAGAATTCATCTACAACGGCATGATGAGAGAGAAAAGCGCTTGAATTTCCCCCAGAGTGTTCGTGACGTGATTTTTGGAAGTGGAGAAATTATTGTTGATGACATGAAGTATACAGTTTCGCATGTGGTAGAACCAGTTGAAAGAGGATTTGCAAAGATAAATAAAACCTTGCATCAGATATCTGAGAATGTGAAACGCCATGAGGAGCAGAGGAGGAGTGCCAAGGATGATAGTTATCTTATCCCCTTGAGTTCATGGAACAGAGAATTTTCTGAAGTCCATGAGAATCTTGTTGAAGGGAGTGTCAGTGATAGTGGTTTGTCCAGTGGGAGGAGACAGCACACCCAGTCTAAAGCTTCAACTTCTCGTCCAAGAGCAGGGAGATGA

Protein Analysis

483

Amino Acids

55.08

Weight (kDa)

6.61

Isoelectric Point (pI)

44.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Solute_trans_a PF03619 43 - 320 2.6e-91 Organic solute transporter Ostalpha
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 503
Acc36I ACCTGC 1 cut(s) 6
AccB1I GGYRCC 1 cut(s) 842
AccB7I CCANNNNNTGG 1 cut(s) 117
AccI GTMKAC 1 cut(s) 1175
AciI CCGC 1 cut(s) 880
AclWI GGATC 2 cut(s) 522, 1025
AcoI YGGCCR 1 cut(s) 119
AcsI RAATTY 4 cut(s) 401, 1068, 1108, 1331
AcuI CTGAAG 1 cut(s) 1359
AfaI GTAC 3 cut(s) 147, 394, 954
AfeI AGCGCT 1 cut(s) 1103
AfiI CCNNNNNNNGG 5 cut(s) 117, 664, 852, 1395, 1442
AflIII ACRYGT 1 cut(s) 72
AgsI TTSAA 9 cut(s) 304, 401, 448, 476, 608, 1108, 1205, 1361, 1425
AhlI ACTAGT 1 cut(s) 148
AjiI CACGTC 1 cut(s) 1132
AloI GAACNNNNNNTCC 2 cut(s) 468, 500
AluBI AGCT 4 cut(s) 117, 194, 871, 1421
AluI AGCT 4 cut(s) 117, 194, 871, 1421
Alw21I GWGCWC 2 cut(s) 189, 437
Alw26I GTCTC 4 cut(s) 55, 63, 501, 1393
AlwI GGATC 2 cut(s) 522, 1025
AlwNI CAGNNNCTG 1 cut(s) 871
Aor51HI AGCGCT 1 cut(s) 1103
AoxI GGCC 5 cut(s) 77, 119, 333, 696, 855
ApaI GGGCCC 1 cut(s) 700
ApeKI GCWGC 3 cut(s) 194, 296, 920
ApoI RAATTY 4 cut(s) 401, 1068, 1108, 1331
ArsI GACNNNNNNTTYG 2 cut(s) 1121, 1153
AspLEI GCGC 4 cut(s) 587, 865, 1024, 1104
AspS9I GGNCC 5 cut(s) 521, 696, 697, 855, 928
AsuC2I CCSGG 2 cut(s) 320, 947
AsuHPI GGTGA 1 cut(s) 395
AsuNHI GCTAGC 1 cut(s) 592
AvaII GGWCC 2 cut(s) 521, 928
BaeGI GKGCMC 1 cut(s) 700
BalI TGGCCA 1 cut(s) 121
BanI GGYRCC 1 cut(s) 842
BanII GRGCYC 1 cut(s) 700
BarI GAAGNNNNNNTAC 2 cut(s) 164, 196
BbsI GAAGAC 1 cut(s) 934
Bbv12I GWGCWC 2 cut(s) 189, 437
BbvI GCAGC 3 cut(s) 181, 283, 907
BccI CCATC 4 cut(s) 119, 238, 904, 991
BceAI ACGGC 2 cut(s) 89, 1097
BcgI CGANNNNNNTGC 2 cut(s) 320, 354
BcnI CCSGG 2 cut(s) 320, 947
BcoDI GTCTC 4 cut(s) 55, 63, 501, 1393
BcuI ACTAGT 1 cut(s) 148
BfaI CTAG 4 cut(s) 149, 263, 593, 1016
BfoI RGCGCY 2 cut(s) 1025, 1105
BfuAI ACCTGC 1 cut(s) 6
BisI GCNGC 3 cut(s) 195, 297, 921
BlsI GCNGC 3 cut(s) 196, 298, 922
Bme1390I CCNGG 2 cut(s) 320, 947
Bme18I GGWCC 2 cut(s) 521, 928
BmgBI CACGTC 1 cut(s) 1132
BmgT120I GGNCC 5 cut(s) 521, 696, 697, 855, 928
BmiI GGNNCC 4 cut(s) 249, 698, 844, 856
BmrFI CCNGG 2 cut(s) 320, 947
BmrI ACTGGG 3 cut(s) 316, 520, 1405
BmsI GCATC 3 cut(s) 561, 1019, 1246
BmtI GCTAGC 1 cut(s) 596
BmuI ACTGGG 3 cut(s) 316, 520, 1405
BpiI GAAGAC 1 cut(s) 934
BplI GAGNNNNNCTC 2 cut(s) 424, 456
BpmI CTGGAG 2 cut(s) 40, 664
Bpu10I CCTNAGC 1 cut(s) 376
BpuEI CTTGAG 1 cut(s) 1333
BpuMI CCSGG 2 cut(s) 320, 947
BsaBI GATNNNNATC 1 cut(s) 1299
BsaI GGTCTC 1 cut(s) 55
BsaJI CCNNGG 2 cut(s) 858, 1287
BsaXI ACNNNNNCTCC 4 cut(s) 206, 236, 1357, 1387
Bsc4I CCNNNNNNNGG 5 cut(s) 117, 664, 852, 1395, 1442
Bse1I ACTGG 7 cut(s) 311, 515, 647, 765, 1199, 1389, 1411
Bse3DI GCAATG 1 cut(s) 991
Bse8I GATNNNNATC 1 cut(s) 1299
BseDI CCNNGG 2 cut(s) 858, 1287
BseGI GGATG 3 cut(s) 11, 478, 1297
BseJI GATNNNNATC 1 cut(s) 1299
BseLI CCNNNNNNNGG 5 cut(s) 117, 664, 852, 1395, 1442
BseMI GCAATG 1 cut(s) 991
BseMII CTCAG 3 cut(s) 103, 142, 1239
BseNI ACTGG 7 cut(s) 311, 515, 647, 765, 1199, 1389, 1411
BseRI GAGGAG 8 cut(s) 43, 227, 429, 1052, 1283, 1291, 1294, 1411
BseSI GKGCMC 1 cut(s) 700
BseXI GCAGC 3 cut(s) 181, 283, 907
BsgI GTGCAG 1 cut(s) 734
BshFI GGCC 5 cut(s) 79, 121, 335, 698, 857
BshNI GGYRCC 1 cut(s) 842
BsiHKAI GWGCWC 2 cut(s) 189, 437
BsiSI CCGG 2 cut(s) 319, 947
BslFI GGGAC 1 cut(s) 335
BslI CCNNNNNNNGG 5 cut(s) 117, 664, 852, 1395, 1442
BsmAI GTCTC 4 cut(s) 55, 63, 501, 1393
BsmFI GGGAC 1 cut(s) 335
BsmI GAATGC 2 cut(s) 299, 527
BsnI GGCC 5 cut(s) 79, 121, 335, 698, 857
Bso31I GGTCTC 1 cut(s) 55
Bsp120I GGGCCC 1 cut(s) 696
Bsp1286I GDGCHC 3 cut(s) 189, 437, 700
Bsp143I GATC 5 cut(s) 228, 514, 579, 1030, 1058
BspACI CCGC 1 cut(s) 880
BspANI GGCC 5 cut(s) 79, 121, 335, 698, 857
BspCNI CTCAG 3 cut(s) 102, 141, 1240
BspLI GGNNCC 4 cut(s) 249, 698, 844, 856
BspMI ACCTGC 1 cut(s) 6
BspOI GCTAGC 1 cut(s) 596
BspPI GGATC 2 cut(s) 522, 1025
BspT107I GGYRCC 1 cut(s) 842
BspTNI GGTCTC 1 cut(s) 55
BsrDI GCAATG 1 cut(s) 991
BsrI ACTGG 7 cut(s) 311, 515, 647, 765, 1199, 1389, 1411
BssECI CCNNGG 2 cut(s) 858, 1287
BssMI GATC 5 cut(s) 228, 514, 579, 1030, 1058
BssNAI GTATAC 1 cut(s) 1176
BssT1I CCWWGG 2 cut(s) 858, 1287
Bst1107I GTATAC 1 cut(s) 1176
Bst4CI ACNGT 3 cut(s) 159, 282, 1180
BstC8I GCNNGC 4 cut(s) 119, 136, 373, 594
BstDEI CTNAG 5 cut(s) 89, 128, 190, 376, 1248
BstF5I GGATG 3 cut(s) 11, 478, 1297
BstH2I RGCGCY 2 cut(s) 1025, 1105
BstHHI GCGC 4 cut(s) 587, 865, 1024, 1104
BstKTI GATC 5 cut(s) 231, 517, 582, 1033, 1061
BstMAI GTCTC 4 cut(s) 55, 63, 501, 1393
BstMBI GATC 5 cut(s) 228, 514, 579, 1030, 1058
BstMWI GCNNNNNNNGC 5 cut(s) 101, 214, 593, 998, 1007
BstNSI RCATGY 3 cut(s) 76, 142, 1190
BstSCI CCNGG 2 cut(s) 318, 945
BstSLI GKGCMC 1 cut(s) 700
BstV1I GCAGC 3 cut(s) 181, 283, 907
BstV2I GAAGAC 1 cut(s) 934
BstX2I RGATCY 1 cut(s) 514
BstYI RGATCY 1 cut(s) 514
BstZ17I GTATAC 1 cut(s) 1176
BsuRI GGCC 5 cut(s) 79, 121, 335, 698, 857
BtrI CACGTC 1 cut(s) 1132
BtsCI GGATG 3 cut(s) 11, 478, 1297
BtsIMutI CAGTG 8 cut(s) 97, 136, 155, 278, 488, 758, 1378, 1396
BveI ACCTGC 1 cut(s) 6
Cac8I GCNNGC 4 cut(s) 119, 136, 373, 594
CaiI CAGNNNCTG 1 cut(s) 871
CfoI GCGC 4 cut(s) 587, 865, 1024, 1104
Cfr13I GGNCC 5 cut(s) 521, 696, 697, 855, 928
Csp6I GTAC 3 cut(s) 146, 393, 953
CviQI GTAC 3 cut(s) 146, 393, 953
DdeI CTNAG 5 cut(s) 89, 128, 190, 376, 1248
DpnI GATC 5 cut(s) 230, 516, 581, 1032, 1060
DpnII GATC 5 cut(s) 228, 514, 579, 1030, 1058
DraI TTTAAA 1 cut(s) 850
EaeI YGGCCR 1 cut(s) 119
Eco130I CCWWGG 2 cut(s) 858, 1287
Eco147I AGGCCT 1 cut(s) 335
Eco24I GRGCYC 1 cut(s) 700
Eco31I GGTCTC 1 cut(s) 55
Eco32I GATATC 1 cut(s) 1245
Eco47I GGWCC 2 cut(s) 521, 928
Eco47III AGCGCT 1 cut(s) 1103
Eco57I CTGAAG 1 cut(s) 1359
EcoO109I RGGNCCY 2 cut(s) 697, 855
EcoRI GAATTC 1 cut(s) 1068
EcoRV GATATC 1 cut(s) 1245
EcoT14I CCWWGG 2 cut(s) 858, 1287
EcoT22I ATGCAT 2 cut(s) 905, 1012
EcoT38I GRGCYC 1 cut(s) 700
ErhI CCWWGG 2 cut(s) 858, 1287
FaqI GGGAC 1 cut(s) 335
FauI CCCGC 1 cut(s) 887
FauNDI CATATG 1 cut(s) 899
FblI GTMKAC 1 cut(s) 1175
Fnu4HI GCNGC 3 cut(s) 195, 297, 921
FokI GGATG 3 cut(s) 18, 465, 1304
FriOI GRGCYC 1 cut(s) 700
Fsp4HI GCNGC 3 cut(s) 195, 297, 921
FspBI CTAG 4 cut(s) 149, 263, 593, 1016
GlaI GCGC 4 cut(s) 586, 864, 1023, 1103
GluI GCNGC 3 cut(s) 195, 297, 921
GsuI CTGGAG 2 cut(s) 40, 664
HaeII RGCGCY 2 cut(s) 1025, 1105
HaeIII GGCC 5 cut(s) 79, 121, 335, 698, 857
HapII CCGG 2 cut(s) 319, 947
HhaI GCGC 4 cut(s) 587, 865, 1024, 1104
Hin6I GCGC 4 cut(s) 585, 863, 1022, 1102
HinP1I GCGC 4 cut(s) 585, 863, 1022, 1102
HindIII AAGCTT 1 cut(s) 1419
HinfI GANTC 7 cut(s) 98, 266, 287, 412, 428, 601, 1351
HpaII CCGG 2 cut(s) 319, 947
HphI GGTGA 1 cut(s) 395
Hpy166II GTNNAC 2 cut(s) 931, 1176
Hpy188I TCNGA 6 cut(s) 243, 417, 690, 1242, 1249, 1339
Hpy188III TCNNGA 5 cut(s) 796, 1028, 1034, 1046, 1127
Hpy8I GTNNAC 2 cut(s) 931, 1176
HpyAV CCTTC 2 cut(s) 623, 1355
HpyCH4III ACNGT 3 cut(s) 159, 282, 1180
HpyCH4IV ACGT 2 cut(s) 964, 1131
HpyCH4V TGCA 8 cut(s) 155, 299, 341, 715, 903, 1010, 1217, 1237
HpyF10VI GCNNNNNNNGC 5 cut(s) 101, 214, 593, 998, 1007
HpyF3I CTNAG 5 cut(s) 89, 128, 190, 376, 1248
HpySE526I ACGT 2 cut(s) 964, 1131
HspAI GCGC 4 cut(s) 585, 863, 1022, 1102
Kzo9I GATC 5 cut(s) 228, 514, 579, 1030, 1058
LmnI GCTCC 4 cut(s) 214, 868, 1019, 1270
Lsp1109I GCAGC 3 cut(s) 181, 283, 907
LweI GCATC 3 cut(s) 561, 1019, 1246
MaeI CTAG 4 cut(s) 149, 263, 593, 1016
MaeII ACGT 2 cut(s) 964, 1131
MaeIII GTNAC 2 cut(s) 276, 1127
MalI GATC 5 cut(s) 230, 516, 581, 1032, 1060
MboI GATC 5 cut(s) 228, 514, 579, 1030, 1058
MboII GAAGA 5 cut(s) 18, 172, 464, 596, 934
MfeI CAATTG 2 cut(s) 396, 786
MflI RGATCY 1 cut(s) 514
MhlI GDGCHC 3 cut(s) 189, 437, 700
MlsI TGGCCA 1 cut(s) 121
MluCI AATT 9 cut(s) 396, 401, 493, 575, 786, 1068, 1108, 1152, 1331
MluNI TGGCCA 1 cut(s) 121
MlyI GAGTC 2 cut(s) 281, 421
MmeI TCCRAC 1 cut(s) 264
Mox20I TGGCCA 1 cut(s) 121
Mph1103I ATGCAT 2 cut(s) 905, 1012
MscI TGGCCA 1 cut(s) 121
MseI TTAA 4 cut(s) 678, 753, 780, 849
MslI CAYNNNNRTG 2 cut(s) 908, 936
Msp20I TGGCCA 1 cut(s) 121
MspA1I CMGCKG 1 cut(s) 117
MspI CCGG 2 cut(s) 319, 947
MspR9I CCNGG 2 cut(s) 320, 947
MunI CAATTG 2 cut(s) 396, 786
Mva1269I GAATGC 2 cut(s) 299, 527
MwoI GCNNNNNNNGC 5 cut(s) 101, 214, 593, 998, 1007
NciI CCSGG 2 cut(s) 320, 947
NdeI CATATG 1 cut(s) 899
NdeII GATC 5 cut(s) 228, 514, 579, 1030, 1058
NheI GCTAGC 1 cut(s) 592
NlaIV GGNNCC 4 cut(s) 249, 698, 844, 856
NmuCI GTSAC 2 cut(s) 276, 1127
NsiI ATGCAT 2 cut(s) 905, 1012
NspI RCATGY 3 cut(s) 76, 142, 1190
PceI AGGCCT 1 cut(s) 335
PciI ACATGT 1 cut(s) 72
PctI GAATGC 2 cut(s) 299, 527
PfeI GAWTC 5 cut(s) 98, 266, 428, 601, 1351
PflMI CCANNNNNTGG 1 cut(s) 117
PfoI TCCNGGA 1 cut(s) 318
PkrI GCNGC 3 cut(s) 196, 298, 922
PleI GAGTC 2 cut(s) 281, 420
PpsI GAGTC 2 cut(s) 281, 420
PscI ACATGT 1 cut(s) 72
PsiI TTATAA 1 cut(s) 503
PspN4I GGNNCC 4 cut(s) 249, 698, 844, 856
PspOMI GGGCCC 1 cut(s) 696
PspPI GGNCC 5 cut(s) 521, 696, 697, 855, 928
PsrI GAACNNNNNNTAC 2 cut(s) 540, 572
PstNI CAGNNNCTG 1 cut(s) 871
PsuI RGATCY 1 cut(s) 514
PvuII CAGCTG 1 cut(s) 117
RsaI GTAC 3 cut(s) 147, 394, 954
RsaNI GTAC 3 cut(s) 146, 393, 953
RseI CAYNNNNRTG 2 cut(s) 908, 936
SaqAI TTAA 4 cut(s) 678, 753, 780, 849
SatI GCNGC 3 cut(s) 195, 297, 921
Sau3AI GATC 5 cut(s) 228, 514, 579, 1030, 1058
Sau96I GGNCC 5 cut(s) 521, 696, 697, 855, 928
SchI GAGTC 2 cut(s) 281, 421
ScrFI CCNGG 2 cut(s) 320, 947
SduI GDGCHC 3 cut(s) 189, 437, 700
SfaNI GCATC 3 cut(s) 561, 1019, 1246
SinI GGWCC 2 cut(s) 521, 928
SmiMI CAYNNNNRTG 2 cut(s) 908, 936
SmlI CTYRAG 1 cut(s) 1312
SmoI CTYRAG 1 cut(s) 1312
SpeI ACTAGT 1 cut(s) 148
Sse9I AATT 9 cut(s) 396, 401, 493, 575, 786, 1068, 1108, 1152, 1331
SseBI AGGCCT 1 cut(s) 335
SsiI CCGC 1 cut(s) 880
SspMI CTAG 4 cut(s) 149, 263, 593, 1016
StuI AGGCCT 1 cut(s) 335
StyD4I CCNGG 2 cut(s) 318, 945
StyI CCWWGG 2 cut(s) 858, 1287
TaaI ACNGT 3 cut(s) 159, 282, 1180
TaiI ACGT 2 cut(s) 967, 1134
TaqI TCGA 3 cut(s) 183, 426, 431
TasI AATT 9 cut(s) 396, 401, 493, 575, 786, 1068, 1108, 1152, 1331
TfiI GAWTC 5 cut(s) 98, 266, 428, 601, 1351
Tru1I TTAA 4 cut(s) 678, 753, 780, 849
Tru9I TTAA 4 cut(s) 678, 753, 780, 849
TscAI CASTG 8 cut(s) 97, 136, 162, 285, 495, 765, 1378, 1396
TseFI GTSAC 2 cut(s) 276, 1127
TseI GCWGC 3 cut(s) 194, 296, 920
Tsp45I GTSAC 2 cut(s) 276, 1127
TspDTI ATGAA 3 cut(s) 1061, 1184, 1308
TspRI CASTG 8 cut(s) 97, 136, 162, 285, 495, 765, 1378, 1396
Van91I CCANNNNNTGG 1 cut(s) 117
VpaK11BI GGWCC 2 cut(s) 521, 928
XapI RAATTY 4 cut(s) 401, 1068, 1108, 1331
XceI RCATGY 3 cut(s) 76, 142, 1190
XmiI GTMKAC 1 cut(s) 1175
XspI CTAG 4 cut(s) 149, 263, 593, 1016
Zsp2I ATGCAT 2 cut(s) 905, 1012
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.