MD05G1037300.v1.1

CS domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0013609
Physical Location & Seq
Reverse (-)
5989698 .. 5992525
2828 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1037300.v1.1.491

Sequence Viewer

Length: 669 bp
ATGAGCCGACATCCTAATGTGAAATGGGCCCAGAGGTCTGATATGGTCTACATAACTATTGACTTGCCGGATGCCCAGGATGTAAAGCTTAAACTGGAGCCTGAAGGAAAGTTTTTGTTCTCTGCTACTACTGGACCAGAGAAGACACCTTATGAAGTTGACCTTGATCTCTATGACAAGATAGATATAAATGAGACCAAGACTAGTGTTGGCTTGAGAAATATCTGTTACCTAGTGAAAAAAGCTGAGGATAAATGGTGGAGCAGGTTGATAAAACAGCAAGGAAGAGCTCCTATTTTTCTGAAAGTGGATTGGGATAAATGGGTTGATGAAGATGAAGAGCCTGAAGAAGTTAAAGGTGACGAAGGCGGTCCAGGTGGGTTTGGAAACAATATGGACTTTGGGGGACTTGGAAACAATATGGACTTTGGAGGACTCGGAAACAATATGAACTTTGGGGGACTGGGAAACAATATGAACTTTGGGGACTTTGATTTGTCTTTGAACATGGGTGGCGATGGCTTGAATGCTGATGCTCTTGGCAAGTTTGATGAGAACGATGACAGTGACGCAGAGGATGAAACTATTGACCAACAACCATCCGCTGCTGGACAATCTGACGCCAAACCTCTTGCGAGTGCGGAGCATGATGCAAAGGCTTCTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00590 ko01100 map00590 map01100
Pfam Domains
Protein Families
CS

Protein Analysis

223

Amino Acids

24.37

Weight (kDa)

4.28

Isoelectric Point (pI)

29.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CS PF04969 5 - 81 2.7e-09 CS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013609)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g12740 FvH4_2g12740
malus_domestica MD05G1037300.v1.1 MD10G1041200.v1.1
prunus_persica Prupe.8G045200_v2.0.a1 Prupe.8G045200_v2.0.a1
pyrus_communis pycom05g02760 pycom10g02830
rosa_chinensis RchiOBHm_Chr6g0273521
rosa_laevigata RLG00000013610
rosa_multiflora Rmu_sc0001419.1_g000004
rosa_roxburghii Rroxscaffold_7G00195260
rosa_rugosa Rorug06G0076000
rosa_samantha Rh6AG190700 Rh6CG193800 Rh6DG185600
rosa_wichuraiana Rw6G005270 Rw6G016690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 255
AccI GTMKAC 1 cut(s) 48
AciI CCGC 3 cut(s) 369, 603, 641
AcuI CTGAAG 2 cut(s) 123, 366
AcyI GRCGYC 1 cut(s) 621
AgsI TTSAA 2 cut(s) 505, 526
AhlI ACTAGT 1 cut(s) 203
AjnI CCWGG 2 cut(s) 75, 373
AluBI AGCT 3 cut(s) 88, 245, 290
AluI AGCT 3 cut(s) 88, 245, 290
Alw21I GWGCWC 1 cut(s) 292
Alw26I GTCTC 1 cut(s) 188
AoxI GGCC 1 cut(s) 27
ApaI GGGCCC 1 cut(s) 31
ApeKI GCWGC 1 cut(s) 605
AspS9I GGNCC 4 cut(s) 27, 28, 134, 371
AsuHPI GGTGA 1 cut(s) 371
AvaII GGWCC 2 cut(s) 134, 371
BaeGI GKGCMC 1 cut(s) 31
BanII GRGCYC 2 cut(s) 31, 292
BbsI GAAGAC 1 cut(s) 149
Bbv12I GWGCWC 1 cut(s) 292
BbvCI CCTCAGC 1 cut(s) 246
BbvI GCAGC 1 cut(s) 592
BccI CCATC 2 cut(s) 512, 607
BciT130I CCWGG 2 cut(s) 77, 375
BcoDI GTCTC 1 cut(s) 188
BcuI ACTAGT 1 cut(s) 203
BfaI CTAG 2 cut(s) 204, 233
BfuAI ACCTGC 1 cut(s) 255
BisI GCNGC 1 cut(s) 606
BlsI GCNGC 1 cut(s) 607
Bme1390I CCNGG 2 cut(s) 77, 375
Bme18I GGWCC 2 cut(s) 134, 371
BmgT120I GGNCC 4 cut(s) 27, 28, 134, 371
BmiI GGNNCC 2 cut(s) 29, 99
BmrFI CCNGG 2 cut(s) 77, 375
BmrI ACTGGG 1 cut(s) 473
BmsI GCATC 3 cut(s) 61, 523, 640
BmuI ACTGGG 1 cut(s) 473
BpiI GAAGAC 1 cut(s) 149
BpmI CTGGAG 1 cut(s) 116
Bpu10I CCTNAGC 1 cut(s) 246
BpuEI CTTGAG 1 cut(s) 235
BsaHI GRCGYC 1 cut(s) 621
BsaI GGTCTC 1 cut(s) 188
BsaJI CCNNGG 1 cut(s) 75
Bse1I ACTGG 3 cut(s) 99, 136, 468
BseBI CCWGG 2 cut(s) 77, 375
BseDI CCNNGG 1 cut(s) 75
BseGI GGATG 5 cut(s) 10, 76, 85, 583, 599
BseMII CTCAG 1 cut(s) 237
BseNI ACTGG 3 cut(s) 99, 136, 468
BseSI GKGCMC 1 cut(s) 31
BseXI GCAGC 1 cut(s) 592
BshFI GGCC 1 cut(s) 29
BsiHKAI GWGCWC 1 cut(s) 292
BsiSI CCGG 1 cut(s) 68
BslFI GGGAC 3 cut(s) 420, 474, 500
BsmAI GTCTC 1 cut(s) 188
BsmFI GGGAC 3 cut(s) 420, 474, 500
BsmI GAATGC 1 cut(s) 532
BsnI GGCC 1 cut(s) 29
Bso31I GGTCTC 1 cut(s) 188
Bsp120I GGGCCC 1 cut(s) 27
Bsp1286I GDGCHC 2 cut(s) 31, 292
Bsp143I GATC 1 cut(s) 166
BspACI CCGC 3 cut(s) 369, 603, 641
BspANI GGCC 1 cut(s) 29
BspCNI CTCAG 1 cut(s) 238
BspLI GGNNCC 2 cut(s) 29, 99
BspMI ACCTGC 1 cut(s) 255
BspQI GCTCTTC 2 cut(s) 280, 333
BspTNI GGTCTC 1 cut(s) 188
BsrI ACTGG 3 cut(s) 99, 136, 468
BssECI CCNNGG 1 cut(s) 75
BssMI GATC 1 cut(s) 166
BssNI GRCGYC 1 cut(s) 621
Bst2UI CCWGG 2 cut(s) 77, 375
Bst4CI ACNGT 1 cut(s) 566
Bst6I CTCTTC 2 cut(s) 280, 333
BstACI GRCGYC 1 cut(s) 621
BstDEI CTNAG 1 cut(s) 246
BstF5I GGATG 5 cut(s) 10, 76, 85, 583, 599
BstKTI GATC 1 cut(s) 169
BstMAI GTCTC 1 cut(s) 188
BstMBI GATC 1 cut(s) 166
BstNI CCWGG 2 cut(s) 77, 375
BstSCI CCNGG 2 cut(s) 75, 373
BstSLI GKGCMC 1 cut(s) 31
BstV1I GCAGC 1 cut(s) 592
BstV2I GAAGAC 1 cut(s) 149
BsuRI GGCC 1 cut(s) 29
BtgZI GCGATG 1 cut(s) 531
BtsCI GGATG 5 cut(s) 10, 76, 85, 583, 599
BtsIMutI CAGTG 1 cut(s) 571
BveI ACCTGC 1 cut(s) 255
Cfr13I GGNCC 4 cut(s) 27, 28, 134, 371
CseI GACGC 2 cut(s) 578, 629
CviAII CATG 2 cut(s) 508, 647
DdeI CTNAG 1 cut(s) 246
DpnI GATC 1 cut(s) 168
DpnII GATC 1 cut(s) 166
Eam1104I CTCTTC 2 cut(s) 280, 333
EarI CTCTTC 2 cut(s) 280, 333
Ecl136II GAGCTC 1 cut(s) 290
Eco24I GRGCYC 2 cut(s) 31, 292
Eco31I GGTCTC 1 cut(s) 188
Eco47I GGWCC 2 cut(s) 134, 371
Eco53kI GAGCTC 1 cut(s) 290
Eco57I CTGAAG 2 cut(s) 123, 366
EcoICRI GAGCTC 1 cut(s) 290
EcoRII CCWGG 2 cut(s) 75, 373
EcoT38I GRGCYC 2 cut(s) 31, 292
FaeI CATG 2 cut(s) 511, 650
FalI AAGNNNNNCTT 2 cut(s) 147, 179
FaqI GGGAC 3 cut(s) 420, 474, 500
FatI CATG 2 cut(s) 507, 646
FblI GTMKAC 1 cut(s) 48
Fnu4HI GCNGC 1 cut(s) 606
FokI GGATG 4 cut(s) 83, 92, 586, 590
FriOI GRGCYC 2 cut(s) 31, 292
Fsp4HI GCNGC 1 cut(s) 606
FspBI CTAG 2 cut(s) 204, 233
GluI GCNGC 1 cut(s) 606
GsuI CTGGAG 1 cut(s) 116
HaeIII GGCC 1 cut(s) 29
HapII CCGG 1 cut(s) 68
HgaI GACGC 2 cut(s) 578, 629
Hin1I GRCGYC 1 cut(s) 621
Hin1II CATG 2 cut(s) 511, 650
HincII GTYRAC 1 cut(s) 160
HindII GTYRAC 1 cut(s) 160
HindIII AAGCTT 1 cut(s) 86
HinfI GANTC 1 cut(s) 435
HpaII CCGG 1 cut(s) 68
HphI GGTGA 1 cut(s) 371
Hpy166II GTNNAC 2 cut(s) 49, 160
Hpy188I TCNGA 4 cut(s) 40, 303, 440, 619
Hpy8I GTNNAC 2 cut(s) 49, 160
HpyAV CCTTC 2 cut(s) 98, 359
HpyCH4III ACNGT 1 cut(s) 566
HpyCH4V TGCA 1 cut(s) 653
HpyF3I CTNAG 1 cut(s) 246
Hsp92I GRCGYC 1 cut(s) 621
Hsp92II CATG 2 cut(s) 511, 650
Kzo9I GATC 1 cut(s) 166
LguI GCTCTTC 2 cut(s) 280, 333
LmnI GCTCC 4 cut(s) 97, 261, 295, 643
Lsp1109I GCAGC 1 cut(s) 592
LweI GCATC 3 cut(s) 61, 523, 640
MaeI CTAG 2 cut(s) 204, 233
MaeIII GTNAC 3 cut(s) 227, 359, 566
MalI GATC 1 cut(s) 168
MboI GATC 1 cut(s) 166
MboII GAAGA 5 cut(s) 154, 297, 344, 350, 359
MhlI GDGCHC 2 cut(s) 31, 292
MlyI GAGTC 1 cut(s) 429
MnlI CCTC 5 cut(s) 27, 241, 425, 568, 639
MseI TTAA 2 cut(s) 90, 354
MslI CAYNNNNRTG 1 cut(s) 15
MspA1I CMGCKG 1 cut(s) 605
MspI CCGG 1 cut(s) 68
MspR9I CCNGG 2 cut(s) 77, 375
Mva1269I GAATGC 1 cut(s) 532
MvaI CCWGG 2 cut(s) 77, 375
NdeII GATC 1 cut(s) 166
NlaIII CATG 2 cut(s) 511, 650
NlaIV GGNNCC 2 cut(s) 29, 99
NmuCI GTSAC 2 cut(s) 359, 566
PciSI GCTCTTC 2 cut(s) 280, 333
PctI GAATGC 1 cut(s) 532
PkrI GCNGC 1 cut(s) 607
PleI GAGTC 1 cut(s) 429
PpsI GAGTC 1 cut(s) 429
Psp124BI GAGCTC 1 cut(s) 292
Psp6I CCWGG 2 cut(s) 75, 373
PspGI CCWGG 2 cut(s) 75, 373
PspN4I GGNNCC 2 cut(s) 29, 99
PspOMI GGGCCC 1 cut(s) 27
PspPI GGNCC 4 cut(s) 27, 28, 134, 371
RseI CAYNNNNRTG 1 cut(s) 15
SacI GAGCTC 1 cut(s) 292
SapI GCTCTTC 2 cut(s) 280, 333
SaqAI TTAA 2 cut(s) 90, 354
SatI GCNGC 1 cut(s) 606
Sau3AI GATC 1 cut(s) 166
Sau96I GGNCC 4 cut(s) 27, 28, 134, 371
SchI GAGTC 1 cut(s) 429
ScrFI CCNGG 2 cut(s) 77, 375
SduI GDGCHC 2 cut(s) 31, 292
SfaNI GCATC 3 cut(s) 61, 523, 640
SinI GGWCC 2 cut(s) 134, 371
SmiMI CAYNNNNRTG 1 cut(s) 15
SmlI CTYRAG 1 cut(s) 214
SmoI CTYRAG 1 cut(s) 214
SpeI ACTAGT 1 cut(s) 203
SsiI CCGC 3 cut(s) 369, 603, 641
SspMI CTAG 2 cut(s) 204, 233
SstI GAGCTC 1 cut(s) 292
StyD4I CCNGG 2 cut(s) 75, 373
TaaI ACNGT 1 cut(s) 566
Tru1I TTAA 2 cut(s) 90, 354
Tru9I TTAA 2 cut(s) 90, 354
TscAI CASTG 1 cut(s) 571
TseFI GTSAC 2 cut(s) 359, 566
TseI GCWGC 1 cut(s) 605
Tsp45I GTSAC 2 cut(s) 359, 566
TspDTI ATGAA 6 cut(s) 168, 345, 351, 464, 491, 594
TspRI CASTG 1 cut(s) 571
VpaK11BI GGWCC 2 cut(s) 134, 371
XmiI GTMKAC 1 cut(s) 48
XspI CTAG 2 cut(s) 204, 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.