MD10G1041200.v1.1

CS domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0013609
Physical Location & Seq
Reverse (-)
5373860 .. 5376813
2954 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1041200.v1.1.491

Sequence Viewer

Length: 693 bp
ATGAGCCGACATCCTAATGTGAAATGGGCCCAGAGGTCTGATATGGTCTACATAACTATTGATTTGCCGGATGCCCAGGATGTAAAGCTTAAACTGGAGCCTGAAGGAAAGTTTTTGTTTTCTGCTACTACTGGACCAGAGAAGACAGCTTATGAAGTTGACCTTGATCTCTATGACAAGATTGATGTAAATGAGACCAAGTCTAGTGTTGGCTTGAGAAATATCTGTTACCTAGTGAAAAAAGCTGAGGAAAAATGGTGGAGCAGGTTGATCAAACAGCAGGGAAAAGCTCCTATTTTTCTGAAAGTGGATTGGGATAAATGGGTTGATGAAGATGAGGAGCCTGAAGAAGTGAAAGGTGACGAAGGCGGTGGGTTTGGAAATAATATGGACTTTGGTGGACTCGGAAACAATATGGACTTTGGAGGACTTGGAAACAATATGGACTTTGGAGGACTCGGAAACAATATGAACCTCGGGGGACTTGGAAACAATATGAACTTTGGGGACTTTGATATGTCTAAGTTGAACATGGGTGGCGGTGGCTCGAGTGCTGATGCTCTTGGCAAGTTTGATGAGAATGATGATAGTGACACAGAGGACGAAACTGCTAACCAACCACCATCCGCTGCTGGAGAATCTGACGCCAAACCTCCTGCGAGTGCTGAGCATGACGCAAAGGCTTCTGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00590 ko01100 map00590 map01100
Pfam Domains
Protein Families
CS

Protein Analysis

231

Amino Acids

25.03

Weight (kDa)

4.34

Isoelectric Point (pI)

34.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CS PF04969 5 - 81 1e-09 CS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013609)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g12740 FvH4_2g12740
malus_domestica MD05G1037300.v1.1 MD10G1041200.v1.1
prunus_persica Prupe.8G045200_v2.0.a1 Prupe.8G045200_v2.0.a1
pyrus_communis pycom05g02760 pycom10g02830
rosa_chinensis RchiOBHm_Chr6g0273521
rosa_laevigata RLG00000013610
rosa_multiflora Rmu_sc0001419.1_g000004
rosa_roxburghii Rroxscaffold_7G00195260
rosa_rugosa Rorug06G0076000
rosa_samantha Rh6AG190700 Rh6CG193800 Rh6DG185600
rosa_wichuraiana Rw6G005270 Rw6G016690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 255
AccI GTMKAC 1 cut(s) 48
AciI CCGC 3 cut(s) 369, 540, 627
AcuI CTGAAG 2 cut(s) 123, 366
AcyI GRCGYC 1 cut(s) 645
AgsI TTSAA 1 cut(s) 529
AjnI CCWGG 1 cut(s) 75
AluBI AGCT 4 cut(s) 88, 149, 245, 290
AluI AGCT 4 cut(s) 88, 149, 245, 290
Alw26I GTCTC 1 cut(s) 188
Ama87I CYCGRG 2 cut(s) 476, 547
AoxI GGCC 1 cut(s) 27
ApaI GGGCCC 1 cut(s) 31
ApeKI GCWGC 1 cut(s) 629
AspS9I GGNCC 3 cut(s) 27, 28, 134
AsuHPI GGTGA 1 cut(s) 371
AvaI CYCGRG 2 cut(s) 476, 547
AvaII GGWCC 1 cut(s) 134
BaeGI GKGCMC 1 cut(s) 31
BanII GRGCYC 1 cut(s) 31
BbsI GAAGAC 1 cut(s) 149
BbvCI CCTCAGC 1 cut(s) 246
BbvI GCAGC 1 cut(s) 616
BccI CCATC 1 cut(s) 631
BciT130I CCWGG 1 cut(s) 77
BclI TGATCA 1 cut(s) 270
BcoDI GTCTC 1 cut(s) 188
BfaI CTAG 2 cut(s) 204, 233
BfuAI ACCTGC 1 cut(s) 255
BisI GCNGC 1 cut(s) 630
BlpI GCTNAGC 1 cut(s) 666
BlsI GCNGC 1 cut(s) 631
Bme1390I CCNGG 1 cut(s) 77
Bme18I GGWCC 1 cut(s) 134
BmeT110I CYCGRG 2 cut(s) 476, 547
BmgT120I GGNCC 3 cut(s) 27, 28, 134
BmiI GGNNCC 3 cut(s) 29, 99, 342
BmrFI CCNGG 1 cut(s) 77
BmsI GCATC 2 cut(s) 61, 547
BpiI GAAGAC 1 cut(s) 149
BpmI CTGGAG 2 cut(s) 116, 654
Bpu10I CCTNAGC 1 cut(s) 246
Bpu1102I GCTNAGC 1 cut(s) 666
BpuEI CTTGAG 1 cut(s) 235
BsaHI GRCGYC 1 cut(s) 645
BsaI GGTCTC 1 cut(s) 188
BsaJI CCNNGG 2 cut(s) 75, 475
Bse1I ACTGG 2 cut(s) 99, 136
BseBI CCWGG 1 cut(s) 77
BseDI CCNNGG 2 cut(s) 75, 475
BseGI GGATG 4 cut(s) 10, 76, 85, 623
BseMII CTCAG 2 cut(s) 237, 657
BseNI ACTGG 2 cut(s) 99, 136
BseRI GAGGAG 1 cut(s) 353
BseSI GKGCMC 1 cut(s) 31
BseXI GCAGC 1 cut(s) 616
BshFI GGCC 1 cut(s) 29
BsiHKCI CYCGRG 2 cut(s) 476, 547
BsiSI CCGG 1 cut(s) 68
BslFI GGGAC 2 cut(s) 495, 521
BsmAI GTCTC 1 cut(s) 188
BsmFI GGGAC 2 cut(s) 495, 521
BsnI GGCC 1 cut(s) 29
Bso31I GGTCTC 1 cut(s) 188
BsoBI CYCGRG 2 cut(s) 476, 547
Bsp120I GGGCCC 1 cut(s) 27
Bsp1286I GDGCHC 1 cut(s) 31
Bsp143I GATC 2 cut(s) 166, 270
Bsp1720I GCTNAGC 1 cut(s) 666
BspACI CCGC 3 cut(s) 369, 540, 627
BspANI GGCC 1 cut(s) 29
BspCNI CTCAG 2 cut(s) 238, 658
BspLI GGNNCC 3 cut(s) 29, 99, 342
BspMI ACCTGC 1 cut(s) 255
BspTNI GGTCTC 1 cut(s) 188
BsrI ACTGG 2 cut(s) 99, 136
BssECI CCNNGG 2 cut(s) 75, 475
BssMI GATC 2 cut(s) 166, 270
BssNI GRCGYC 1 cut(s) 645
Bst2UI CCWGG 1 cut(s) 77
BstACI GRCGYC 1 cut(s) 645
BstDEI CTNAG 3 cut(s) 246, 522, 666
BstF5I GGATG 4 cut(s) 10, 76, 85, 623
BstKTI GATC 2 cut(s) 169, 273
BstMAI GTCTC 1 cut(s) 188
BstMBI GATC 2 cut(s) 166, 270
BstNI CCWGG 1 cut(s) 77
BstSCI CCNGG 1 cut(s) 75
BstSLI GKGCMC 1 cut(s) 31
BstV1I GCAGC 1 cut(s) 616
BstV2I GAAGAC 1 cut(s) 149
BsuRI GGCC 1 cut(s) 29
BtsCI GGATG 4 cut(s) 10, 76, 85, 623
BveI ACCTGC 1 cut(s) 255
Cfr13I GGNCC 3 cut(s) 27, 28, 134
CseI GACGC 2 cut(s) 653, 683
CviAII CATG 2 cut(s) 532, 671
DdeI CTNAG 3 cut(s) 246, 522, 666
DpnI GATC 2 cut(s) 168, 272
DpnII GATC 2 cut(s) 166, 270
Eco24I GRGCYC 1 cut(s) 31
Eco31I GGTCTC 1 cut(s) 188
Eco47I GGWCC 1 cut(s) 134
Eco57I CTGAAG 2 cut(s) 123, 366
Eco88I CYCGRG 2 cut(s) 476, 547
EcoRII CCWGG 1 cut(s) 75
EcoT38I GRGCYC 1 cut(s) 31
FaeI CATG 2 cut(s) 535, 674
FalI AAGNNNNNCTT 2 cut(s) 147, 179
FaqI GGGAC 2 cut(s) 495, 521
FatI CATG 2 cut(s) 531, 670
FbaI TGATCA 1 cut(s) 270
FblI GTMKAC 1 cut(s) 48
Fnu4HI GCNGC 1 cut(s) 630
FokI GGATG 3 cut(s) 83, 92, 610
FriOI GRGCYC 1 cut(s) 31
Fsp4HI GCNGC 1 cut(s) 630
FspBI CTAG 2 cut(s) 204, 233
GluI GCNGC 1 cut(s) 630
GsuI CTGGAG 2 cut(s) 116, 654
HaeIII GGCC 1 cut(s) 29
HapII CCGG 1 cut(s) 68
HgaI GACGC 2 cut(s) 653, 683
Hin1I GRCGYC 1 cut(s) 645
Hin1II CATG 2 cut(s) 535, 674
HincII GTYRAC 1 cut(s) 160
HindII GTYRAC 1 cut(s) 160
HindIII AAGCTT 1 cut(s) 86
HinfI GANTC 3 cut(s) 402, 456, 638
HpaII CCGG 1 cut(s) 68
HphI GGTGA 1 cut(s) 371
Hpy166II GTNNAC 3 cut(s) 49, 160, 401
Hpy188I TCNGA 6 cut(s) 40, 303, 407, 461, 643, 692
Hpy8I GTNNAC 3 cut(s) 49, 160, 401
HpyAV CCTTC 2 cut(s) 98, 359
HpyF3I CTNAG 3 cut(s) 246, 522, 666
Hsp92I GRCGYC 1 cut(s) 645
Hsp92II CATG 2 cut(s) 535, 674
Ksp22I TGATCA 1 cut(s) 270
Kzo9I GATC 2 cut(s) 166, 270
LmnI GCTCC 4 cut(s) 97, 261, 295, 340
Lsp1109I GCAGC 1 cut(s) 616
LweI GCATC 2 cut(s) 61, 547
MaeI CTAG 2 cut(s) 204, 233
MaeIII GTNAC 3 cut(s) 227, 359, 590
MalI GATC 2 cut(s) 168, 272
MboI GATC 2 cut(s) 166, 270
MboII GAAGA 3 cut(s) 154, 344, 359
MhlI GDGCHC 1 cut(s) 31
MlyI GAGTC 2 cut(s) 396, 450
MnlI CCTC 8 cut(s) 27, 241, 331, 419, 446, 485, 592, 663
MseI TTAA 1 cut(s) 90
MslI CAYNNNNRTG 1 cut(s) 15
MspA1I CMGCKG 1 cut(s) 629
MspI CCGG 1 cut(s) 68
MspR9I CCNGG 1 cut(s) 77
MvaI CCWGG 1 cut(s) 77
NdeII GATC 2 cut(s) 166, 270
NlaIII CATG 2 cut(s) 535, 674
NlaIV GGNNCC 3 cut(s) 29, 99, 342
NmuCI GTSAC 2 cut(s) 359, 590
PaeR7I CTCGAG 1 cut(s) 547
PfeI GAWTC 1 cut(s) 638
PflFI GACNNNGTC 1 cut(s) 199
PkrI GCNGC 1 cut(s) 631
PleI GAGTC 2 cut(s) 396, 450
PpsI GAGTC 2 cut(s) 396, 450
Psp6I CCWGG 1 cut(s) 75
PspGI CCWGG 1 cut(s) 75
PspN4I GGNNCC 3 cut(s) 29, 99, 342
PspOMI GGGCCC 1 cut(s) 27
PspPI GGNCC 3 cut(s) 27, 28, 134
PspXI VCTCGAGB 1 cut(s) 547
PsyI GACNNNGTC 1 cut(s) 199
RseI CAYNNNNRTG 1 cut(s) 15
SaqAI TTAA 1 cut(s) 90
SatI GCNGC 1 cut(s) 630
Sau3AI GATC 2 cut(s) 166, 270
Sau96I GGNCC 3 cut(s) 27, 28, 134
SchI GAGTC 2 cut(s) 396, 450
ScrFI CCNGG 1 cut(s) 77
SduI GDGCHC 1 cut(s) 31
SfaNI GCATC 2 cut(s) 61, 547
Sfr274I CTCGAG 1 cut(s) 547
SinI GGWCC 1 cut(s) 134
SlaI CTCGAG 1 cut(s) 547
SmiMI CAYNNNNRTG 1 cut(s) 15
SmlI CTYRAG 2 cut(s) 214, 547
SmoI CTYRAG 2 cut(s) 214, 547
SsiI CCGC 3 cut(s) 369, 540, 627
SspMI CTAG 2 cut(s) 204, 233
StyD4I CCNGG 1 cut(s) 75
TaqI TCGA 1 cut(s) 548
TfiI GAWTC 1 cut(s) 638
Tru1I TTAA 1 cut(s) 90
Tru9I TTAA 1 cut(s) 90
TseFI GTSAC 2 cut(s) 359, 590
TseI GCWGC 1 cut(s) 629
Tsp45I GTSAC 2 cut(s) 359, 590
TspDTI ATGAA 4 cut(s) 168, 345, 485, 512
Tth111I GACNNNGTC 1 cut(s) 199
VpaK11BI GGWCC 1 cut(s) 134
XhoI CTCGAG 1 cut(s) 547
XmiI GTMKAC 1 cut(s) 48
XspI CTAG 2 cut(s) 204, 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.