MD05G1058700.v1.1

Protein SHI RELATED SEQUENCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0009392
Physical Location & Seq
Forward (+)
10147514 .. 10148466
953 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1058700.v1.1.491

Sequence Viewer

Length: 699 bp
ATGGTGAGGCAAGGAAGAGGAGGAAGAGAAGAAGAAATAATGGGTTCCAGATGCCAAGACTGTGGGAATCAAGCTAAGAAGGAGTGTGCGTACTTGAGATGCAGGACTTGCTGTAAAAGCAAAGGATTTCACTGCCAAACTCACGTCAAGAGTACTTGGGTTCCTGTCTACAGAAGGCGCCATACTCATCACCAACAGCTTCCTTCTATTGATCCACAGCACCCAGCTCACCTTCATCAAGGGCACAGCTACAACCCTAAGAGGCCAGCAGCCGGACACGTCATCACCAATCCCTCTTCATCTGGGCAAGATCAACAGGTTAACAATTTTCCAGCAGAAGTGAATTCCATGGCCACTTTTCGGTGTGTTCGAGTGAGCTCCGTGGAGGATACGGTCAATCAGCACGCTTATCAGACAAGTGTGGTAATCGGAGGGCATGTATTCAAAGGTATACTCAATGATCAAGGCCCTAATTACACTACTGCAGCTGGCCAAAGCTCCAGCCCCCAGCATGTAATGAATAAGCAAACCAGCAATTTTATTAGTGCTGCTGCTTCTGCTTCGAATATTACTGCTTCTACTTCATCAGCTGCTGCAGATCATCAAGGGCCACATCATCATCAACCTCCTTCTTCATGTCCATTGATTCCCTTTAATGCTTTCATGCCTGGTACGCAATTTTTCCTTAACCCAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF702

Protein Analysis

233

Amino Acids

25.4

Weight (kDa)

9.26

Isoelectric Point (pI)

50.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF702 PF05142 15 - 158 4.3e-55 Domain of unknown function (DUF702)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 177
AccI GTMKAC 2 cut(s) 168, 451
AclWI GGATC 1 cut(s) 206
AcoI YGGCCR 2 cut(s) 351, 490
AcsI RAATTY 1 cut(s) 343
AcyI GRCGYC 1 cut(s) 178
AfaI GTAC 3 cut(s) 92, 154, 673
AfiI CCNNNNNNNGG 2 cut(s) 272, 360
AflIII ACRYGT 1 cut(s) 277
AgsI TTSAA 1 cut(s) 445
AjiI CACGTC 2 cut(s) 145, 280
AjnI CCWGG 1 cut(s) 667
AluBI AGCT 8 cut(s) 74, 199, 227, 249, 378, 488, 498, 590
AluI AGCT 8 cut(s) 74, 199, 227, 249, 378, 488, 498, 590
Alw21I GWGCWC 1 cut(s) 380
AlwI GGATC 1 cut(s) 206
AlwNI CAGNNNCTG 1 cut(s) 593
AoxI GGCC 5 cut(s) 263, 351, 466, 490, 608
ApeKI GCWGC 6 cut(s) 269, 485, 548, 551, 590, 593
ApoI RAATTY 1 cut(s) 343
AspLEI GCGC 1 cut(s) 180
AspS9I GGNCC 2 cut(s) 467, 608
AsuHPI GGTGA 4 cut(s) 16, 182, 221, 277
AsuII TTCGAA 1 cut(s) 563
BaeGI GKGCMC 1 cut(s) 246
BalI TGGCCA 2 cut(s) 353, 492
BanI GGYRCC 1 cut(s) 177
BanII GRGCYC 1 cut(s) 380
BarI GAAGNNNNNNTAC 2 cut(s) 166, 198
Bbv12I GWGCWC 1 cut(s) 380
BbvI GCAGC 6 cut(s) 281, 497, 535, 538, 577, 580
BciT130I CCWGG 1 cut(s) 669
BciVI GTATCC 1 cut(s) 382
BclI TGATCA 1 cut(s) 460
BfmI CTRYAG 3 cut(s) 169, 483, 594
BfoI RGCGCY 1 cut(s) 181
BfuI GTATCC 1 cut(s) 382
BisI GCNGC 6 cut(s) 270, 486, 549, 552, 591, 594
BlsI GCNGC 6 cut(s) 271, 487, 550, 553, 592, 595
BmcAI AGTACT 1 cut(s) 154
Bme1390I CCNGG 1 cut(s) 669
BmgBI CACGTC 2 cut(s) 145, 280
BmgT120I GGNCC 2 cut(s) 467, 608
BmiI GGNNCC 3 cut(s) 46, 162, 179
BmrFI CCNGG 1 cut(s) 669
BmsI GCATC 2 cut(s) 41, 89
BpmI CTGGAG 1 cut(s) 484
Bpu14I TTCGAA 1 cut(s) 563
BpuEI CTTGAG 1 cut(s) 115
BsaHI GRCGYC 1 cut(s) 178
BsaJI CCNNGG 2 cut(s) 348, 381
BsaXI ACNNNNNCTCC 4 cut(s) 74, 104, 423, 453
Bsc4I CCNNNNNNNGG 2 cut(s) 272, 360
BseBI CCWGG 1 cut(s) 669
BseDI CCNNGG 2 cut(s) 348, 381
BseLI CCNNNNNNNGG 2 cut(s) 272, 360
BseRI GAGGAG 1 cut(s) 33
BseSI GKGCMC 1 cut(s) 246
BseXI GCAGC 6 cut(s) 281, 497, 535, 538, 577, 580
BseYI CCCAGC 2 cut(s) 223, 507
BshFI GGCC 5 cut(s) 265, 353, 468, 492, 610
BshNI GGYRCC 1 cut(s) 177
BsiHKAI GWGCWC 1 cut(s) 380
BsiSI CCGG 1 cut(s) 273
BslI CCNNNNNNNGG 2 cut(s) 272, 360
BsnI GGCC 5 cut(s) 265, 353, 468, 492, 610
Bsp119I TTCGAA 1 cut(s) 563
Bsp1286I GDGCHC 2 cut(s) 246, 380
Bsp143I GATC 4 cut(s) 211, 310, 460, 598
Bsp19I CCATGG 1 cut(s) 348
BspANI GGCC 5 cut(s) 265, 353, 468, 492, 610
BspLI GGNNCC 3 cut(s) 46, 162, 179
BspMAI CTGCAG 2 cut(s) 487, 598
BspPI GGATC 1 cut(s) 206
BspT104I TTCGAA 1 cut(s) 563
BspT107I GGYRCC 1 cut(s) 177
BssECI CCNNGG 2 cut(s) 348, 381
BssMI GATC 4 cut(s) 211, 310, 460, 598
BssNAI GTATAC 1 cut(s) 452
BssNI GRCGYC 1 cut(s) 178
BssT1I CCWWGG 1 cut(s) 348
Bst1107I GTATAC 1 cut(s) 452
Bst2UI CCWGG 1 cut(s) 669
Bst4CI ACNGT 2 cut(s) 62, 394
Bst6I CTCTTC 3 cut(s) 10, 19, 301
BstACI GRCGYC 1 cut(s) 178
BstAPI GCANNNNNTGC 1 cut(s) 108
BstBI TTCGAA 1 cut(s) 563
BstC8I GCNNGC 3 cut(s) 267, 405, 490
BstDEI CTNAG 2 cut(s) 75, 258
BstDSI CCRYGG 2 cut(s) 348, 381
BstH2I RGCGCY 1 cut(s) 181
BstHHI GCGC 1 cut(s) 180
BstKTI GATC 4 cut(s) 214, 313, 463, 601
BstMBI GATC 4 cut(s) 211, 310, 460, 598
BstMWI GCNNNNNNNGC 4 cut(s) 108, 117, 557, 673
BstNI CCWGG 1 cut(s) 669
BstNSI RCATGY 2 cut(s) 440, 515
BstSCI CCNGG 1 cut(s) 667
BstSFI CTRYAG 3 cut(s) 169, 483, 594
BstSLI GKGCMC 1 cut(s) 246
BstV1I GCAGC 6 cut(s) 281, 497, 535, 538, 577, 580
BstXI CCANNNNNNTGG 1 cut(s) 62
BstZ17I GTATAC 1 cut(s) 452
BsuI GTATCC 1 cut(s) 382
BsuRI GGCC 5 cut(s) 265, 353, 468, 492, 610
BtgI CCRYGG 2 cut(s) 348, 381
BtrI CACGTC 2 cut(s) 145, 280
BtsI GCAGTG 1 cut(s) 130
BtsIMutI CAGTG 1 cut(s) 130
Cac8I GCNNGC 3 cut(s) 267, 405, 490
CaiI CAGNNNCTG 1 cut(s) 593
CfoI GCGC 1 cut(s) 180
Cfr13I GGNCC 2 cut(s) 467, 608
Csp6I GTAC 3 cut(s) 91, 153, 672
CviAII CATG 5 cut(s) 349, 437, 512, 636, 664
CviQI GTAC 3 cut(s) 91, 153, 672
DdeI CTNAG 2 cut(s) 75, 258
DinI GGCGCC 1 cut(s) 179
DpnI GATC 4 cut(s) 213, 312, 462, 600
DpnII GATC 4 cut(s) 211, 310, 460, 598
EaeI YGGCCR 2 cut(s) 351, 490
Eam1104I CTCTTC 3 cut(s) 10, 19, 301
EarI CTCTTC 3 cut(s) 10, 19, 301
Ecl136II GAGCTC 1 cut(s) 378
Eco130I CCWWGG 1 cut(s) 348
Eco24I GRGCYC 1 cut(s) 380
Eco53kI GAGCTC 1 cut(s) 378
EcoICRI GAGCTC 1 cut(s) 378
EcoO109I RGGNCCY 1 cut(s) 467
EcoRI GAATTC 1 cut(s) 343
EcoRII CCWGG 1 cut(s) 667
EcoT14I CCWWGG 1 cut(s) 348
EcoT38I GRGCYC 1 cut(s) 380
EgeI GGCGCC 1 cut(s) 179
EheI GGCGCC 1 cut(s) 179
ErhI CCWWGG 1 cut(s) 348
FaeI CATG 5 cut(s) 352, 440, 515, 639, 667
FaiI YATR 7 cut(s) 183, 350, 438, 452, 513, 637, 665
FatI CATG 5 cut(s) 348, 436, 511, 635, 663
FbaI TGATCA 1 cut(s) 460
FblI GTMKAC 2 cut(s) 168, 451
Fnu4HI GCNGC 6 cut(s) 270, 486, 549, 552, 591, 594
FriOI GRGCYC 1 cut(s) 380
Fsp4HI GCNGC 6 cut(s) 270, 486, 549, 552, 591, 594
GlaI GCGC 1 cut(s) 179
GluI GCNGC 6 cut(s) 270, 486, 549, 552, 591, 594
GsaI CCCAGC 2 cut(s) 227, 511
GsuI CTGGAG 1 cut(s) 484
HaeII RGCGCY 1 cut(s) 181
HaeIII GGCC 5 cut(s) 265, 353, 468, 492, 610
HapII CCGG 1 cut(s) 273
HhaI GCGC 1 cut(s) 180
Hin1I GRCGYC 1 cut(s) 178
Hin1II CATG 5 cut(s) 352, 440, 515, 639, 667
Hin6I GCGC 1 cut(s) 178
HinP1I GCGC 1 cut(s) 178
HincII GTYRAC 1 cut(s) 322
HindII GTYRAC 1 cut(s) 322
HinfI GANTC 2 cut(s) 67, 646
HpaI GTTAAC 1 cut(s) 322
HpaII CCGG 1 cut(s) 273
HphI GGTGA 4 cut(s) 16, 182, 221, 277
Hpy166II GTNNAC 3 cut(s) 169, 322, 452
Hpy188I TCNGA 2 cut(s) 414, 431
Hpy188III TCNNGA 2 cut(s) 48, 148
Hpy8I GTNNAC 3 cut(s) 169, 322, 452
HpyAV CCTTC 5 cut(s) 73, 168, 213, 242, 639
HpyCH4III ACNGT 2 cut(s) 62, 394
HpyCH4IV ACGT 2 cut(s) 144, 279
HpyCH4V TGCA 3 cut(s) 102, 485, 596
HpyF10VI GCNNNNNNNGC 4 cut(s) 108, 117, 557, 673
HpyF3I CTNAG 2 cut(s) 75, 258
HpySE526I ACGT 2 cut(s) 144, 279
Hsp92I GRCGYC 1 cut(s) 178
Hsp92II CATG 5 cut(s) 352, 440, 515, 639, 667
HspAI GCGC 1 cut(s) 178
KasI GGCGCC 1 cut(s) 177
Ksp22I TGATCA 1 cut(s) 460
KspAI GTTAAC 1 cut(s) 322
Kzo9I GATC 4 cut(s) 211, 310, 460, 598
LmnI GCTCC 2 cut(s) 383, 503
Lsp1109I GCAGC 6 cut(s) 281, 497, 535, 538, 577, 580
LweI GCATC 2 cut(s) 41, 89
MaeII ACGT 2 cut(s) 144, 279
MalI GATC 4 cut(s) 213, 312, 462, 600
MboI GATC 4 cut(s) 211, 310, 460, 598
MboII GAAGA 6 cut(s) 27, 36, 41, 44, 288, 624
MhlI GDGCHC 2 cut(s) 246, 380
MlsI TGGCCA 2 cut(s) 353, 492
MluCI AATT 5 cut(s) 325, 343, 472, 535, 677
MluNI TGGCCA 2 cut(s) 353, 492
Mly113I GGCGCC 1 cut(s) 178
MnlI CCTC 7 cut(s) 11, 14, 255, 304, 379, 425, 636
Mox20I TGGCCA 2 cut(s) 353, 492
MscI TGGCCA 2 cut(s) 353, 492
MseI TTAA 3 cut(s) 321, 654, 687
Msp20I TGGCCA 2 cut(s) 353, 492
MspA1I CMGCKG 2 cut(s) 488, 590
MspI CCGG 1 cut(s) 273
MspR9I CCNGG 1 cut(s) 669
MvaI CCWGG 1 cut(s) 669
MwoI GCNNNNNNNGC 4 cut(s) 108, 117, 557, 673
NarI GGCGCC 1 cut(s) 178
NcoI CCATGG 1 cut(s) 348
NdeII GATC 4 cut(s) 211, 310, 460, 598
NlaIII CATG 5 cut(s) 352, 440, 515, 639, 667
NlaIV GGNNCC 3 cut(s) 46, 162, 179
NspI RCATGY 2 cut(s) 440, 515
NspV TTCGAA 1 cut(s) 563
PcsI WCGNNNNNNNCGW 1 cut(s) 367
PfeI GAWTC 2 cut(s) 67, 646
PkrI GCNGC 6 cut(s) 271, 487, 550, 553, 592, 595
PluTI GGCGCC 1 cut(s) 181
Psp124BI GAGCTC 1 cut(s) 380
Psp6I CCWGG 1 cut(s) 667
PspFI CCCAGC 2 cut(s) 223, 507
PspGI CCWGG 1 cut(s) 667
PspN4I GGNNCC 3 cut(s) 46, 162, 179
PspPI GGNCC 2 cut(s) 467, 608
PstI CTGCAG 2 cut(s) 487, 598
PstNI CAGNNNCTG 1 cut(s) 593
PvuII CAGCTG 2 cut(s) 488, 590
RsaI GTAC 3 cut(s) 92, 154, 673
RsaNI GTAC 3 cut(s) 91, 153, 672
SacI GAGCTC 1 cut(s) 380
SaqAI TTAA 3 cut(s) 321, 654, 687
SatI GCNGC 6 cut(s) 270, 486, 549, 552, 591, 594
Sau3AI GATC 4 cut(s) 211, 310, 460, 598
Sau96I GGNCC 2 cut(s) 467, 608
ScaI AGTACT 1 cut(s) 154
ScrFI CCNGG 1 cut(s) 669
SduI GDGCHC 2 cut(s) 246, 380
SfaNI GCATC 2 cut(s) 41, 89
SfcI CTRYAG 3 cut(s) 169, 483, 594
SfoI GGCGCC 1 cut(s) 179
SfuI TTCGAA 1 cut(s) 563
SmlI CTYRAG 1 cut(s) 94
SmoI CTYRAG 1 cut(s) 94
Sse9I AATT 5 cut(s) 325, 343, 472, 535, 677
SspDI GGCGCC 1 cut(s) 177
SspI AATATT 1 cut(s) 568
SstI GAGCTC 1 cut(s) 380
StyD4I CCNGG 1 cut(s) 667
StyI CCWWGG 1 cut(s) 348
TaaI ACNGT 2 cut(s) 62, 394
TaiI ACGT 2 cut(s) 147, 282
TaqI TCGA 2 cut(s) 370, 563
TasI AATT 5 cut(s) 325, 343, 472, 535, 677
TatI WGTACW 1 cut(s) 152
TfiI GAWTC 2 cut(s) 67, 646
Tru1I TTAA 3 cut(s) 321, 654, 687
Tru9I TTAA 3 cut(s) 321, 654, 687
TscAI CASTG 1 cut(s) 137
TseI GCWGC 6 cut(s) 269, 485, 548, 551, 590, 593
TspDTI ATGAA 6 cut(s) 224, 288, 533, 573, 624, 652
TspGWI ACGGA 1 cut(s) 370
TspRI CASTG 1 cut(s) 137
XapI RAATTY 1 cut(s) 343
XceI RCATGY 2 cut(s) 440, 515
XmiI GTMKAC 2 cut(s) 168, 451
ZrmI AGTACT 1 cut(s) 154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.