Rh6BG150100

Protein SHI RELATED SEQUENCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0009392
Physical Location & Seq
Reverse (-)
24142278 .. 24152052
9775 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG150100.1

Sequence Viewer

Length: 498 bp
ATGCAGGCAGATGCCCTTGAATCTCCACCTCCGTGGCTACTGATCGACTGCGATTTATATTTGAAGAAGAAAGGCAGGATTAACAGGTGGTCGGCTCTGTTGTACTTGTCCACACTGAGGGGTCATATCCCTCAAAAGCTCAAATCGAAATGTACCAACAAGGAATTGTGCAGATGTGTAGGGCTAGATCGAGATCATCAAGAGGCTAATTTTCCGGCTGAAGTGACTACCATGGCCAAATTTCAGGGTATTCGAGTGAGCTCCATGGACGATATGGTGGATCTGGATCAGCATGCTTATCAGACAAGCGTGATTATAGGTGGCCATGTATTCAGGGGCATTCTCTATAATCAAGGCCCTGCTGTTCATCAGATGAACTATTACACTTCAGCCTCTGCTGCAGATCAACCACATCATCCTCCTTCATATCAATTGATTAATCCATTTAACGCTTTCATGCCTGCAGGTACACAATTTTTCCAAAACCTATCATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF702

Protein Analysis

165

Amino Acids

18.63

Weight (kDa)

7.73

Isoelectric Point (pI)

35.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF702 PF05142 63 - 120 8.2e-17 Domain of unknown function (DUF702)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 455
AclWI GGATC 2 cut(s) 288, 294
AcoI YGGCCR 2 cut(s) 234, 322
AcsI RAATTY 1 cut(s) 239
AcuI CTGAAG 2 cut(s) 240, 372
AfaI GTAC 3 cut(s) 104, 154, 469
AfiI CCNNNNNNNGG 1 cut(s) 117
AgsI TTSAA 2 cut(s) 20, 64
AleI CACNNNNGTG 1 cut(s) 31
AluBI AGCT 2 cut(s) 139, 261
AluI AGCT 2 cut(s) 139, 261
Alw21I GWGCWC 1 cut(s) 263
AlwI GGATC 2 cut(s) 288, 294
AlwNI CAGNNNCTG 1 cut(s) 395
AoxI GGCC 3 cut(s) 234, 322, 355
ApeKI GCWGC 1 cut(s) 398
ApoI RAATTY 1 cut(s) 239
AseI ATTAAT 1 cut(s) 438
AspS9I GGNCC 1 cut(s) 356
BalI TGGCCA 2 cut(s) 236, 324
BanII GRGCYC 1 cut(s) 263
Bbv12I GWGCWC 1 cut(s) 263
BbvI GCAGC 1 cut(s) 385
BfaI CTAG 1 cut(s) 185
BfmI CTRYAG 2 cut(s) 399, 462
BfuAI ACCTGC 1 cut(s) 455
BisI GCNGC 1 cut(s) 399
BlsI GCNGC 1 cut(s) 400
BmgT120I GGNCC 1 cut(s) 356
BsaBI GATNNNNATC 2 cut(s) 192, 285
BsaJI CCNNGG 3 cut(s) 32, 231, 264
Bsc4I CCNNNNNNNGG 1 cut(s) 117
Bse8I GATNNNNATC 2 cut(s) 192, 285
BseDI CCNNGG 3 cut(s) 32, 231, 264
BseGI GGATG 1 cut(s) 415
BseJI GATNNNNATC 2 cut(s) 192, 285
BseLI CCNNNNNNNGG 1 cut(s) 117
BseMII CTCAG 1 cut(s) 107
BseXI GCAGC 1 cut(s) 385
BsgI GTGCAG 1 cut(s) 190
BshFI GGCC 3 cut(s) 236, 324, 357
BsiHKAI GWGCWC 1 cut(s) 263
BsiSI CCGG 1 cut(s) 215
BslI CCNNNNNNNGG 1 cut(s) 117
BsmI GAATGC 1 cut(s) 339
BsnI GGCC 3 cut(s) 236, 324, 357
Bsp1286I GDGCHC 1 cut(s) 263
Bsp143I GATC 6 cut(s) 42, 187, 193, 280, 286, 403
Bsp19I CCATGG 2 cut(s) 231, 264
BspANI GGCC 3 cut(s) 236, 324, 357
BspCNI CTCAG 1 cut(s) 108
BspMAI CTGCAG 2 cut(s) 403, 466
BspMI ACCTGC 1 cut(s) 455
BspPI GGATC 2 cut(s) 288, 294
BssECI CCNNGG 3 cut(s) 32, 231, 264
BssMI GATC 6 cut(s) 42, 187, 193, 280, 286, 403
BssT1I CCWWGG 2 cut(s) 231, 264
BstC8I GCNNGC 3 cut(s) 6, 294, 462
BstDEI CTNAG 1 cut(s) 116
BstDSI CCRYGG 3 cut(s) 32, 231, 264
BstF5I GGATG 1 cut(s) 415
BstKTI GATC 6 cut(s) 45, 190, 196, 283, 289, 406
BstMBI GATC 6 cut(s) 42, 187, 193, 280, 286, 403
BstMWI GCNNNNNNNGC 1 cut(s) 398
BstNSI RCATGY 1 cut(s) 296
BstSFI CTRYAG 2 cut(s) 399, 462
BstV1I GCAGC 1 cut(s) 385
BstX2I RGATCY 1 cut(s) 280
BstXI CCANNNNNNTGG 1 cut(s) 33
BstYI RGATCY 1 cut(s) 280
BsuRI GGCC 3 cut(s) 236, 324, 357
BtgI CCRYGG 3 cut(s) 32, 231, 264
BtsCI GGATG 1 cut(s) 415
BtsIMutI CAGTG 1 cut(s) 113
BveI ACCTGC 1 cut(s) 455
Cac8I GCNNGC 3 cut(s) 6, 294, 462
CaiI CAGNNNCTG 1 cut(s) 395
Cfr13I GGNCC 1 cut(s) 356
Csp6I GTAC 3 cut(s) 103, 153, 468
CviAII CATG 5 cut(s) 232, 265, 293, 326, 457
CviQI GTAC 3 cut(s) 103, 153, 468
DdeI CTNAG 1 cut(s) 116
DpnI GATC 6 cut(s) 44, 189, 195, 282, 288, 405
DpnII GATC 6 cut(s) 42, 187, 193, 280, 286, 403
EaeI YGGCCR 2 cut(s) 234, 322
Ecl136II GAGCTC 1 cut(s) 261
Eco130I CCWWGG 2 cut(s) 231, 264
Eco24I GRGCYC 1 cut(s) 263
Eco53kI GAGCTC 1 cut(s) 261
Eco57I CTGAAG 2 cut(s) 240, 372
EcoICRI GAGCTC 1 cut(s) 261
EcoO109I RGGNCCY 1 cut(s) 356
EcoT14I CCWWGG 2 cut(s) 231, 264
EcoT38I GRGCYC 1 cut(s) 263
ErhI CCWWGG 2 cut(s) 231, 264
FaeI CATG 5 cut(s) 235, 268, 296, 329, 460
FatI CATG 5 cut(s) 231, 264, 292, 325, 456
Fnu4HI GCNGC 1 cut(s) 399
FokI GGATG 1 cut(s) 402
FriOI GRGCYC 1 cut(s) 263
Fsp4HI GCNGC 1 cut(s) 399
FspBI CTAG 1 cut(s) 185
GluI GCNGC 1 cut(s) 399
HaeIII GGCC 3 cut(s) 236, 324, 357
HapII CCGG 1 cut(s) 215
Hin1II CATG 5 cut(s) 235, 268, 296, 329, 460
HinfI GANTC 1 cut(s) 20
HpaII CCGG 1 cut(s) 215
Hpy166II GTNNAC 2 cut(s) 111, 470
Hpy188I TCNGA 2 cut(s) 303, 372
Hpy188III TCNNGA 3 cut(s) 191, 200, 284
Hpy8I GTNNAC 2 cut(s) 111, 470
HpyAV CCTTC 1 cut(s) 432
HpyCH4V TGCA 4 cut(s) 4, 171, 401, 464
HpyF10VI GCNNNNNNNGC 1 cut(s) 398
HpyF3I CTNAG 1 cut(s) 116
Hsp92II CATG 5 cut(s) 235, 268, 296, 329, 460
Kzo9I GATC 6 cut(s) 42, 187, 193, 280, 286, 403
LmnI GCTCC 1 cut(s) 266
LpnPI CCDG 9 cut(s) 61, 70, 228, 230, 269, 319, 372, 450, 474
Lsp1109I GCAGC 1 cut(s) 385
MaeI CTAG 1 cut(s) 185
MaeIII GTNAC 1 cut(s) 223
MalI GATC 6 cut(s) 44, 189, 195, 282, 288, 405
MboI GATC 6 cut(s) 42, 187, 193, 280, 286, 403
MboII GAAGA 2 cut(s) 76, 79
MfeI CAATTG 1 cut(s) 431
MflI RGATCY 1 cut(s) 280
MhlI GDGCHC 1 cut(s) 263
MlsI TGGCCA 2 cut(s) 236, 324
MluCI AATT 5 cut(s) 164, 208, 239, 431, 473
MluNI TGGCCA 2 cut(s) 236, 324
MnlI CCTC 6 cut(s) 39, 111, 141, 196, 403, 429
Mox20I TGGCCA 2 cut(s) 236, 324
MscI TGGCCA 2 cut(s) 236, 324
MseI TTAA 4 cut(s) 81, 438, 447, 496
MslI CAYNNNNRTG 1 cut(s) 31
Msp20I TGGCCA 2 cut(s) 236, 324
MspI CCGG 1 cut(s) 215
MunI CAATTG 1 cut(s) 431
Mva1269I GAATGC 1 cut(s) 339
MwoI GCNNNNNNNGC 1 cut(s) 398
NcoI CCATGG 2 cut(s) 231, 264
NdeII GATC 6 cut(s) 42, 187, 193, 280, 286, 403
NlaIII CATG 5 cut(s) 235, 268, 296, 329, 460
NmuCI GTSAC 1 cut(s) 223
NspI RCATGY 1 cut(s) 296
OliI CACNNNNGTG 1 cut(s) 31
PaeI GCATGC 1 cut(s) 296
PctI GAATGC 1 cut(s) 339
PfeI GAWTC 1 cut(s) 20
PkrI GCNGC 1 cut(s) 400
PshBI ATTAAT 1 cut(s) 438
Psp124BI GAGCTC 1 cut(s) 263
PspPI GGNCC 1 cut(s) 356
PstI CTGCAG 2 cut(s) 403, 466
PstNI CAGNNNCTG 1 cut(s) 395
PsuI RGATCY 1 cut(s) 280
RsaI GTAC 3 cut(s) 104, 154, 469
RsaNI GTAC 3 cut(s) 103, 153, 468
RseI CAYNNNNRTG 1 cut(s) 31
SacI GAGCTC 1 cut(s) 263
SaqAI TTAA 4 cut(s) 81, 438, 447, 496
SatI GCNGC 1 cut(s) 399
Sau3AI GATC 6 cut(s) 42, 187, 193, 280, 286, 403
Sau96I GGNCC 1 cut(s) 356
SbfI CCTGCAGG 1 cut(s) 466
SdaI CCTGCAGG 1 cut(s) 466
SduI GDGCHC 1 cut(s) 263
SetI ASST 7 cut(s) 31, 89, 141, 263, 322, 469, 489
SfcI CTRYAG 2 cut(s) 399, 462
SmiMI CAYNNNNRTG 1 cut(s) 31
SphI GCATGC 1 cut(s) 296
Sse8387I CCTGCAGG 1 cut(s) 466
Sse9I AATT 5 cut(s) 164, 208, 239, 431, 473
SspMI CTAG 1 cut(s) 185
SstI GAGCTC 1 cut(s) 263
StyI CCWWGG 2 cut(s) 231, 264
TaqI TCGA 4 cut(s) 45, 146, 190, 253
TasI AATT 5 cut(s) 164, 208, 239, 431, 473
TatI WGTACW 1 cut(s) 102
TfiI GAWTC 1 cut(s) 20
Tru1I TTAA 4 cut(s) 81, 438, 447, 496
Tru9I TTAA 4 cut(s) 81, 438, 447, 496
TscAI CASTG 1 cut(s) 120
TseFI GTSAC 1 cut(s) 223
TseI GCWGC 1 cut(s) 398
Tsp45I GTSAC 1 cut(s) 223
TspDTI ATGAA 4 cut(s) 356, 389, 414, 445
TspGWI ACGGA 1 cut(s) 21
TspRI CASTG 1 cut(s) 120
VspI ATTAAT 1 cut(s) 438
XapI RAATTY 1 cut(s) 239
XceI RCATGY 1 cut(s) 296
XcmI CCANNNNNNNNNTGG 1 cut(s) 271
XspI CTAG 1 cut(s) 185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.